Investigation of Genetic Diversity in Some Aloe Sp. Accessions Using Morphological Markers in the in vitro conditions

mansoor saljooghianpour ¹ ( Department of Agriculture, Iranshahr Branch, Islamic Azad University, Iranshahr, Iran )
s. mahdi javadzadeh ² ( Department of Agriculture, Iranshahr Branch, Islamic Azad University, Iranshahr, Iran )
Ahmad Ebrahimi ³ ( Agronomy - Agriculture - IAU - Iranshahr - Iran )

Submited date : 2019-06-26 Accepted date : 2020-09-13

Keywords: Cluster analysis, genetic diversity, tissue culture, Morphological Characteristics, aloe vera,

Abstract :

For study the genetic diversity of Aloe germplasm and identify the classification and relationship of germplasm collections and exploiting them in breeding programs, Thirty three Aloe accessions were collected. These accessions were including 31 wild genotypes and 2 commercial genotypes. Shoot tip explants were washed and sterile. Then, were inoculated on solid MS medium supplemented with 0.5 mgl-1 benzyl adenine + 0.5 mgl-1 α-naphthalene acetic acid and sub-cultured on the same medium for plantlet production and propagation once every four weeks. Plantlets were grown in Completely Randomized Design (CRD) with four replications in greenhouse. After six months, 18 morphological traits were measured and analyzed. Morphological analysis indicated that all the studied various characters have a significant difference at P < 0.01 among Aloe accessions. Also comparison of the means (Duncan’s test) for the genotypes was performed. Maximum amounts for most traits was related to genotype 29 which suggests the potential of this genotype for further studies. The results of orthogonal comparisons between genotypes of wild or native genotypes in a group and the control group showed that the genotypes for most morphological traits studied, there was no significant difference. Cluster analysis indicated that all accessions were divided into three major groups. There were commercial accessions in the third group, and the best wild accession (number of 29) was in this group that is representing high potential of this genotype.