Intra-species genetic diversity between different populations of Meloidogyne javanica on tomato in North Khorasan province useing RAPD -PCR

Subject Areas : Plant Protection

Ghasem Fadavi Khalajlo ¹ , Esmat Mahdikhani Moghadam ² , Hamid Rouhani ³

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Received: 2013-07-02 Accepted : 2014-08-05 Published : 2014-11-22

Keywords:

Abstract :

In order to identify of root-knot nematodes in tomato of North Khorasan province, during 2009-2010, 21 populations of M. javanica collected from roots of tomato plants. Morphological studies on perineal patterns of adult females and morphometrical characters of J2s did not revealed conspectus polymorphism between different populations of M. javanica. In order to investigating genetic diversity of M. javanica, purification and multiplication of each population were done on susceptible tomato (Red colud cultivar). Eggs and second stage juveniles were isolated from each root and supposed one population. Genomic DNA extracted Morphological studies were assessed by species specific primers. The genetic diversity of populations were studied by Random Amplified Polymorphic DNA (RAPD) with ten 10 mer primers. After amplification, PCR-products were separated by electrophoresis on 1.7℅ gel. DNA bands were scored as 1(presence band) or 0(absence band) in excel and NTSYS software by UPGMA method and dendrograms were constructed based on cluster analysis. The clustering at the 73℅ disagreement level revealed five groups. Based on the global result, RAPD marker could show 73 percent similarity and 27 percent difference between populations.

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