تنوع ژنتیکی بین گاوهای Bali (Bos javanicus) و هیبریدهای آن‌ها با استفاده از نشانگرهای ریزماهواره

Subject Areas : Camel

ج. جاکاریا ¹ , ا. آلیاه ² , ف. سپوترا ³ , م. بیهقی ⁴ , ر.ر. نور ⁵

1 - Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor University, Bogor, Indonesia
2 - Goat Research Station, Sei Putih, North Sumatera, Indonesia
3 - Indonesian Research Institute for Animal Production, Bogor, Indonesia
4 - Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor University, Bogor, Indonesia
5 - Department of Animal Production and Technology, Faculty of Animal Science, Institut Pertanian Bogor University, Bogor, Indonesia

Received: 2019-08-30 Accepted : 2019-10-30 Published : 2020-09-01

Keywords: تنوع ژنتیکی, نشانگرهای ریزماهواره, گاوهای Bali,

Abstract :

هدف از این مطالعه ارزیابی تنوع ژنتیکی گاوهای Bali و گاوهای هیبرید با استفاده از نشانگرهای ریزماهواره بود. نمونه‌های خون (192n=) از نژادهای گاو متفاوت جمع­آوری شد، برای نمونه Bali (n=96)، Madura (n=46)، وPeranakan Ongole (PO) Kebumen (n=48). جایگاه ژنی ریزماهواره استفاده شده برای دست‌یابی به تنوع ژنتیکی INRA035، ILSTS006، ETH225 و HEL9 بود، اگرچه پروفایل ژنتیکی با استفاده از برنامه‌های GenAlEX، Cervus، MEGA6، structure و R توضیح داده شدند. به عنوان یک نتیجه، 46 آلل در چهار لوکوس ریزماهواره مطالعه­شده یافت شد. تنوع ژنتیکی گاوهای Bali از جزیره Bali (H0=0.337) و جزیره Nusa Penida (H0=0.375) پایین‌تر از هیبریدها رکوردبرداری شدند، برای نمونه گاوهای نژاد Madura (H0=0.474) و PO Kebumen (H0=0.567). اختصاصاً، ما یافتیم 283 جفت باز (لوکوس ILST006)، 194 جفت بازی (لوکوس ETH225)، 147 جفت بازی و 151 جفت بازی (لوکوس HEL9) نشان دهنده آلل‌های متمایز برای گاوهای Bali. همچنین، داده‌های آزمایشگاهی ما نشان داد که نشانگرهای ریزماهواره استفاده شده اجازه می‌دهد به ما برای تولید یک تفرق آشکار از خوشه گاوها بین گاوهای Bali و هیبریدها، که برای برنامه‌های اصلاح نژادی آینده گاوها معنی‌دار بود.

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