تنوع میتوکندری و ساختار فیلوژنتیکی جمعیت بز مرخز
Subject Areas : Camelح.ر. سیدآبادی 1 , ک. پهلوان افشاری 2 , م. عبدالمالکی 3
1 - Department of Animal Biotechnology, Animal Science Research Institute, Karaj, Iran
2 - Department of Animal Science, Abhar Branch, Islamic Azad University, Abhar, Iran
3 - Department of Animal Science, Abhar Branch, Islamic Azad University, Abhar, Iran
Keywords: تنوع ژنتیکی, بز مرخز, DNA میتوکندری, فیلوژنتیک,
Abstract :
تنوع میتوکندری و ساختار فیلوژنتیکی جمعیت بز مرخز بر اساس توالی DNA میتوکندریایی مورد بررسی قرار گرفت. آنالیز فیلوژنتیکی بر اساس توالی ناحیه HVR1 ( 968 جفت باز) و بر روی 40 رأس بز مرخز انجام گرفت. نتایج نشان داد که جمعیت بز مرخز دارای تنوع ژنتیکی بسیار بالا ( میانگین تنوع هاپلوتیپی 999/0 و تنوع نوکلئوتیدی 022/0) میباشد. در بین 40 نمونه، 6 هاپلوتیپ مشاهده شد و بزرگترین هاپلوتیپ شامل 15 فرد بود. تمام هاپلوتیپها در هاپلوگروپ C و با فاصله ژنتیکی زیاد از سایر جمعیتهای بز آسیایی قرار گرفتند که این بیانگر تنوع ژنتیکی زیاد بز مرخز میباشد. تنوع ژنتیکی بالای مشاهده شده، در اصلاح نژاد و تعیین استراتژیهای حفاظت بز مرخز میتواند مؤثر باشد.
Agaviezor B.O., Adefenwa M.A., Peters S.O., Yakubu A., Adebambo O.A., Ozoje M.O., Ikeobi C.O.N., Ilori B.M., Wheto M., Ajayi O.O., Amusan S.A., Okpeku M., De Donato M. and Imumorin I.G. (2012). Genetic diversity analysis of the mitochondrial D-loop of Nigerian indigenous sheep. Anim. Gen. Resour. 50, 13-20.
ArnasonU., Gullberg A., Johnsson E. and Ledje C. (1993). The nucleotide sequence of the mitochondrial DNA molecule of the grey seal, Halichoerus grypus and a comparison with the mitochondrial sequences of other true seals. J. Mol. Evol. 37, 323-330.
ArnasonU., Gullberg A. and Widegren B. (1991). The complete nucleotide sequence of the mitochondrial DNA of the ¢n whale Balaenoptera physalus. J. Mol. Evol. 33, 556-568.
Ameur A., Stewart J.B., Freyer C., Hagstro E., Ingman M., Ran N., Larsson G. and GyllenstenU. (2011). Ultra-deep sequencing of mouse mitochondrial DNA: mutational patterns and their origins. PLoS Gent. 7, 1-15.
Anderson S., DeBruijin M.H.L., Colson A.R., Eperon I.C., Sanger F. and Young I.G. (1982). Complete sequence of bovine mitochondrial DNA conserved features of the mammalian mitochondrial genome. J. Mol. Biol. 156, 683-692.
Brown W.M., George M.J. and Wilson AC. (1979). Rapid evolution of animal mitochondrial DNA. Proc. Nat. Acad. Sci. USA. 76, 1967-1971.
Chen S.Y., Su Y.H., Wu S.F., Sha T. and Zhang Y.P. (2005). Mitochondrial diversity and phylogeographic structure of Chinese domestic goats. Mol. Phylogenet. Evol. 73, 804-814.
Çinar Kul B. and Ertugrul O. (2011). mtDNA diversity and phylogeography of some Turkish native goat breeds. Ankara Üniv. Vet. Fak. Derg. 58, 129-134.
Cozzi M.C., Strillacci M.G., Valianti P., Bighignoli B., Cancedda M. and Zanotti M. (2004). Mitochondrial D-loop sequence variation among Italian horse breeds. Genet. Sel. Evol. 36, 663-672.
Ghivizzani S.C., Madsen C.S. and Hauswirth W.M. (1993). In organello footprinting: analysis of protein binding at regulatory regions in bovine mitochondrial DNA. J. Biol. Chem. 268, 8675-8682.
Hall T.A. (1999). Bio edit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids. Symp. Ser. 41, 95-98.
Hoda A., Bicoku Y. and Dobi P. (2014). Genetic diversity of Albanian goat breeds revealed by mtDNA sequence variation. Biotechnol. Biotec. Equip. 28, 77-81.
Hoque M.R., Choi N.R., Sultana H., Kang B.S., Heo K.N., Hong S.K., Jo C. and Lee J.H. (2013). Phylogenetic analysis of a privately-owned Korean Native chicken population using mtDNA D-loop variations. Asian-Australas J. Anim. Sci. 26, 157-162.
Ilie D.E., Cean A., Cziszter L.T., Gavojdian D., Ivan A. and Kusza S. (2015). Microsatellite and mitochondrial DNA study of native eastern European cattle populations: the case of the Romanian Grey. PLoS One. 10, 1-18.
Joshi M.B., Rout P.K., Mandal A.K., Tyler-Smith C., Singh L. and Thangaraj K. (2004). Phylogeography and origin of Indian domestic goats. Mol. Biol. Evol. 21, 454-462.
Kumar S., Tamura K., Jakobsen I.B. and Nei M. (2004). MEGA3.1: Molecular Evolutionary Genetics Analysis Software. ArizonaStateUniversity Press, Tempe, USA.
Li K.Y., Li K.T., Cheng C.C., Chen C.H., Hung C.Y. and Ju Y.T. (2014). A genetic analysis of Taoyuan pig and its phylogenetic relationship to Eurasian pig breeds. Asian-Australas J. Anim. Sci. 28, 457-466.
Liu Z.G., Lei C.Z., Luo J., Ding C., Chen G.H., Chang H., Wang K.H., Liu X.X., Zhang X.Y., Xiao X.J. and Wu S.L. (2006). Genetic variability of mtDNA sequences in Chinese native chicken breeds. Asian-Australas J. Anim. Sci. 17, 903-909.
Luikart G., Gielly L., Excoffier L., Vigne J.D., Bouvet J. and Taberlet P. (2001). Multiple maternal origins and weak phylogeographic structure in domestic goats. Proc. Nat. Acad. Sci. USA. 98, 5927-5932.
Mignotte F., Gueride M., Champagne A.M. and Mounolou J.C. (1990). Direct repeats in the non-coding region of mitochondrial DNA. Involvement in the generation of intra and inter-individual heterogeneity. European J. Biochem. 194, 561-571.
Moradi M.H., Rostamzadeh J., Rashidi A., Vahabi K. and Farahmand H. (2014). Analysis of genetic diversity in Iranian mohair goat and its color types using Inter Simple Sequence Repeat (ISSR) markers. Agric. Commod. 2, 55-62.
Naderi S., Rezaei H.R., Taberlet P., Zundel S., Rafat S.A., Naghash H.R., Elbarody M.A.A., Ertugrul O. and Pompanon F. (2007). Large-scale mitochondrial DNA analysis of the domestic goat reveals six haplogroups with high diversity. PloS One. 2, 1-10.
Nass M.M. (1995). Precise sequence assignment of replication origin in the control region of chick mitochondrial DNA relative to 5َ and 3َ ends, secondary structure, DNA synthesis and protein binding. Curr. Genet. 28, 401-409.
Pakpahan S., Tunas Artama W., Widayanti R. and Suparta G. (2015). Genetic variations and the origin of native Indonesian goat breeds based on mtDNA D-Loop sequences. Asia J. Anim. Sci. 9, 341-350.
Rozas J., Sachez-Delbarrio J.C., Messeguer X. and Rozas R. (2003). DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics. 19, 2496-2497.
Saccone C., Pesole G. and Sbisa E. (1991). The main regulatory region of mammalian mitochondrial DNA: structure function model and evolutionary pattern. J. Mol. Evol. 33, 83-91.
Saitou N. and Nei M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.
Sultana S., Mannen H. and Tsuji S. (2003). Mitochondrial DNA diversity of Pakistani goats. Anim. Genet. 34, 417-421.
Taberlet P., Valentini A., Rezaei H.R., Naderi S., Pompanon F., Negrini R. and Ajmone-Marsan P. (2008). Are cattle, sheep, and goats endangered species. Mol. Ecol. 17, 275-284.
Tamura K. and Nei M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10, 512-526.
Wilson A.C., Cann R.L., Carr S.M., George M., Gyllensten U.B., Helm-Bychowski K.M., Higuchi R.J., Palumbi S.R., PragerE.M., Sage R.D. and Stoneking M. (1985). Mitochondrial DNA and two perspectives on evolutionary genetics. Biol. J. Linn. Soc. 26, 375-400.
Xu X., Gullberg A. and ArnasonU. (1996). The complete mitochondrial DNA (mtDNA) of the donkey and mtDNA comparisons among four closely related mammalian species pairs. J. Mol. Evol. 43, 438-446.
Zernekova C., Kott T. and Majzlik I. (2013). Mitochondrial D-loop sequence variation among Hucul horse. Czech J. Anim. Sci. 58, 437-442.