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      • Open Access Article

        1 - Determine optimum of harvest time on the quantity/quality of essential oil and thymol of thyme (Thymus vulgaris L.) in Isfahan
        امین هادی پناه احمدرضا گل پرور عبدالله قاسمی پیربلوطی حسین زینلی
        Background & Aim: Thyme is common named Thymus vulgaris L. belongs to Lamiaceae family. Thyme is a most important medicinal plant that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed countries.Experimental: Determine optimum of h More
        Background & Aim: Thyme is common named Thymus vulgaris L. belongs to Lamiaceae family. Thyme is a most important medicinal plant that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed countries.Experimental: Determine optimum of harvest time this plant was done in a random block design with three replications was conducted in field Islamic Azad University of Khorasgan (Isfahan) 2009 and 2010. Treatment phonological stages such as: 1) before flowering stage; 2) at beginning of flowering stage; 3) 50% blooming stage; 4) at full flowering step and 5) at fruit set stage. The essential oil obtained by hydro-distillation using a Clevenger- type apparatus and, was analyzed by GC and GC/MS.Results & Discussion: Statistic analysis showed that the effects phonological stages had significant effect on essential oil yields & percent, thymol. The highest essential oil content (2.42%) was extracted at the beginning of flowering stage. Analysis and identification of components showed thymol main compounds in all samples.Industrial/Practical recommendation: According to the results of this project 50% blooming stage optimum of harvest time on the Quantity/Quality of Essence and thymol of Thyme. Manuscript profile
      • Open Access Article

        2 - The effect of Hypericum scabrum on the hematologic factors, body weight and temperature in mice
        عبدالله قاسمی پیربلوطی محسن جعفریان دهکردی رحمان پورحکیم داودی بهزاد حامدی محمد ربیعی
        Background & Aim: Vast variety of plants in various regions of the country and lack of enough information on their chemical combinations, sometimes lead to triggering poisoning and unsatisfied complications. Regarding to fabulous herbal characteristics of Hypricum, More
        Background & Aim: Vast variety of plants in various regions of the country and lack of enough information on their chemical combinations, sometimes lead to triggering poisoning and unsatisfied complications. Regarding to fabulous herbal characteristics of Hypricum, many research about poisoning and side effects of high concentrations of H. scabrum have not taken place until now. Therefore regarding to above mentioned issues, current study has done due to evaluating poisoning effects of H. scabrum on behavioral properties of mice.Experimental: In an experimental study, first H. scabrum collected from Sabz-e-Kooh mountains, Chaharmahal va Bakhtiari province. There were five 4 mice-group that has taken 100, 250, 500 and 1000 mg/kg H. scabrum extract and one group didn’t get anything. Lastly, after 24 hours various hematologic, weight, and temperature characteristics have been evaluated. Also, like the past method these properties were evaluated for 14 days after taking extracts. Results & Discussion: In pathologic evaluation of kidney and liver, no obvious difference was seen. In statistical analysis of the results about HCT, Hb, RBC, Mono, MCV, Band, MCH, MCHC and PH no significant differences obtained between case groups and control group. About WBC, neutrophils and eosinophils count significant difference was gathered (p<0.05), that the reason was physiologic stress due to high dosage of the drug; because band cells (immature neutrophils), marker of infection and bone marrow response, has not decreased.Industrial and practical recommendations: Totally in current study it seems that H. scabrum extract, in none of the concentration used, have poisoning effect and just can lead to stress profile in subjects. But regarding the body weights after 2 weeks of experiment, it can be concluded probably the plant was lead to weight gain. Entirely H. scabrum had not any harmful effects but pathologic ones on liver in high concentrations. Manuscript profile
      • Open Access Article

        3 - Effect of Cichorium intybus L. extracts and Kelussia oderatassima Mozaff. essential oil on toxic of organophosphouros insecticides in RAT
        عبدالله قاسمی پیربلوطی علی شاه ولی فیروزه سقایی شهرزاد عزیزی بهزاد حامدی لهراسب شاهقلیان
        Background & Aim: Organophosphates are frequently used as insecticides in households, in agricultural areas and veterinary, thus posing a risk for accidental dermal exposure or through inhalation. Acute organophosphate poisoning causes thousands of human mortalities More
        Background & Aim: Organophosphates are frequently used as insecticides in households, in agricultural areas and veterinary, thus posing a risk for accidental dermal exposure or through inhalation. Acute organophosphate poisoning causes thousands of human mortalities each year in developing countries. Material & Methods: This study carried out to study an antitoxic effect of endemic Iranian medicinal plants agent against organophosphorus on rats. In order, anti-toxic effects of Cichorium intybus L. root ethanol extract and Kelussia oderatassima Mozaff. leaves essential oil on liver and kidney rat. This is an experimental study in 18 Wistar rats were randomly into 6 groups. After 24 hr fasting, normal saline was given to the first group, two and three groups received 200 and 400 mg/kg Cichorium intybus extract, four and five groups received 200 and 400 mg/kg Kelussia oderatassima essential oil and six groups received standard drug (Phenobarbital). Results & Discussion: The histopathological observation indicated that the lowest necrosis in kidney was group received 200 mg/kg Kelussia oderatassima essential oil and highest necrosis in kidney was groups received 200 and 400 mg/kg Cichorium intybus extract and standard drug. The histopathological observation indicated that standard drug and groups received 400 mg/kg Cichorium intybus extract and 200 mg/kg Kelussia oderatassima essential oil may protect liver against acute hepatotoxicity induced by Organophosphate insecticide. Also the highest of increased weight rats revealed group received 200 mg/kg Kelussia oderatassima essential oil. Conclusion: The results show that Kelussia oderatassima essential oil an antitoxic effect against organophosphrus on at. Manuscript profile
      • Open Access Article

        4 - Antibacterial activity of the essential oils of endemic plants
        شهره دادفر عبدالله قاسمی پیربلوطی مریم میرلوحی محمد حجت الاسلامی بهزاد حامدی
        Background & Aim: Secondary metabolites with bioactive and functional properties in essence of aromatic plants can be useful for improvement of quality and persistence of food as natural preservative. The aim of this study was to evaluate the in vitro antibacterial More
        Background & Aim: Secondary metabolites with bioactive and functional properties in essence of aromatic plants can be useful for improvement of quality and persistence of food as natural preservative. The aim of this study was to evaluate the in vitro antibacterial of essential oils of some of the medicinal plants collected from Chaharmahal va Bakhtiari Province, Iran.Experimental: The essential oil of Iranian medicinal plants including Thymus daenensis Celak, T. carmanicus Jalals, Satureja bachtiarica Bunge, S. khuzestanica Jamzad and S hortensis L (Lamiceae) and Kelussia odoratissima Mozaff and Heracleum lasiopetalum Boiss were collected from southwest Iran. Plant materials were subjected to hydro-distillation using a Clevenger-type apparatus according to the method recommended in BT. The antibacterial activity of the essential oils against Pseudomonas aeruginosa was tested by agar disc diffusion.Results & Discussion: The results showed that almost studied essential oils from seven herbs showed antibacterial activity. The essential oils of various species of Lamiaceae have higher antibacterial activity than Apieacea species.Industrial and practical recommendations: In finally, the results obtained appeared to confirm the antibacterial potential of the plants investigated. Satureja species (savory) essential oils can be replaced the natural flavor preservative by synthetic preservative in food industry especially in production of meat. Manuscript profile
      • Open Access Article

        5 - Effect of Bakhtiari celery (Kelussia odoratissma Mozaff) on sensory properties and shelf life of set yogurt
        امیر شاکریان محمد جواد سهرابی عبدالله قاسمی پیربلوطی
        Background & Aim: The essential oils are the most important secondary aromatic biochemical compounds in plants and even in some animals. They are of great importance because of their aromatic, flavoring and preventing properties from deterioration in food industries More
        Background & Aim: The essential oils are the most important secondary aromatic biochemical compounds in plants and even in some animals. They are of great importance because of their aromatic, flavoring and preventing properties from deterioration in food industries. According to ethnobotany study of aerial parts of Bakhtiari celery or Kelus (Kelussia odoratissma Mozaffarian) in dairy such as yogurt and buttermilk, encouraged us to do this research. Experimental: In this study, the essential oil of K. odoratissima Mozaff was extracted. Evolution was made of three concentrations of essential oil (20, 40 and 60 ppm) which added to yogurt after inoculation. The yogurt made by industrial method. During 30 days with certain intervals physical, chemical and microbial properties were analyzed.Results & Discussion: The results showed that essential oil of K. odoratissima Mozaff has improved significantly physical and chemical properties of yogurt. It led to increasing sensory properties, acidity control, improving flavor of yogurt and increasing time of consumption but water holding capacity (WHC) was reduced.Industrial and practical recommendations: It was proved that the best concentration of essential oil of K. odoratissima Mozaff was 40 ppm. Manuscript profile
      • Open Access Article

        6 - Effect of jasmonic (JA) and salicylic acid (SA) on phytochemical properties of Salvia officinalis L.
        مهدی رحیم ملک شکوفه آزاد مهراب یادگاری عبدالله قاسمی پیربلوطی
        Background & aim: Sage (Salvia officinalis L.) is a perennial medicinal herb which belongs to Lamiales order and Lamiaceae family. This plant has economical value for four years. Nowadays the use of plant growth regulators (PGR) has an important role for increasing More
        Background & aim: Sage (Salvia officinalis L.) is a perennial medicinal herb which belongs to Lamiales order and Lamiaceae family. This plant has economical value for four years. Nowadays the use of plant growth regulators (PGR) has an important role for increasing the secondary metabolites in plants. Among PGRs Jasmonic (JA) and salicylic acid (SA) have many applications in medicinal plants.Experimental: This study was carried out to survey the effect of spraying of various concentrations JA and SA on S. officinalis L. in field experiment of Hormozgan, South Iran at 2010-2011. A pot experiment was conducted in a CRD with ten treatments with three replicates. The treatments including I: distilled water (control), II: distilled water and solution (acetone), III-VI: SA in different concentrations (1, 10, 20 and 40 mol/l, VII-X: JA in various concentrations including 50, 100, 200 and 400 µM. The leaves were harvested at before flowering and, the essential oil extracted by Clevenger approach then the compounds were characterized using Gas Chromatography–Mass Spectrometry (GC-MS).Results & Discussion: Results showed that various concentrations of foliar application of JA and SA lead to decrease or increasing of some secondary metabolites in essential oil of S. officinalis L. Compounds such as a-pinene, camphene, b-pinene, 1,8-cineole, cis-thujane, trans-thujane, borneol, bornyl acetate and camphor were affected from JA and SA treatments.Industrial and practical recommendations: According to the results of present study it suggests to increase the major compounds of essential oil of Salvia officinalis L.using SA and JA. Manuscript profile
      • Open Access Article

        7 - An overview on genus Thymus
        عبدالله قاسمی پیربلوطی زهره امامی بیستگانی فاطمه ملک پور
        The genus Thymus L. belongs to the Lamiaceae family, consist of about 215 species of herbaceous perennials and small shrubs in the world. They originated from Mediterranean region. This genus is presented in Iranian flora by 14 species, including: T. daenensis,T. carman More
        The genus Thymus L. belongs to the Lamiaceae family, consist of about 215 species of herbaceous perennials and small shrubs in the world. They originated from Mediterranean region. This genus is presented in Iranian flora by 14 species, including: T. daenensis,T. carmanicus, T. fallax, T. persicus, T. trautvetteri, T. migricus, T. kotschyanus,T. pubesens, T. nummularius, T. transcaspicus, T. eriocalyx, T. caucasicus, T. transcaucasicus, and T. fedtschenkoi. Thyme is an aromatic and medicinal herb that has been widely used in folk medicine, food preservatives and pharmaceutical preparations. Overall, this genus is one of the most popular plants throughout the entire world due to its volatile constituents. Thymol and carvacrol are the major compounds in most of the Thymus essential oils. The therapeutic potential of thyme rests on contents of thymol, carvacrol, flavonoids, eugenol, aliphatic phenols as well as luteolin, saponins, and tetra methoxylated flavones. The essential oil of thyme has antibacterial, antiseptic, antifungal, anti-parasitic and antioxidant activity. Manuscript profile
      • Open Access Article

        8 - Phytochemistry analysis of essential oils of Heracleum lasiopetalum fruits, Thymus carmanicus aerial parts, and Myrtus communis leaves
        لیلا صداقت بروجنی محمد حجت الاسلامی عبدالله قاسمی پیربلوطی هومان مولوی
        Background & Aim: The use of herbs in traditional medicine to treatment of diseases and other purposes in the food industry and cosmetics has since long been considered. Considering thatHeracleum lasiopetalum, Thymus carmanicus and Myrtus communis have many health b More
        Background & Aim: The use of herbs in traditional medicine to treatment of diseases and other purposes in the food industry and cosmetics has since long been considered. Considering thatHeracleum lasiopetalum, Thymus carmanicus and Myrtus communis have many health benefits therefore this research were performed to investigate the chemical composition of essential oils from these plants. Experimental: The essential oil from the fruits of H. lasiopetalum, aerial parts of T. carmanicus and leaves of M. communis were extracted using a Clevenger-type apparatus and analyzed by GC/MS. Also content of the extracted essential oils were calculated. Results & Discussion: Results indicated the amount of essential oils of H. lasiopetalum, T. carmanicus M. communis were significant and they had volatile. The main constituents of essential oils were hydrocarbons, aldehydes, ketones, alcohols, phenols, ethers with phenolic and terpenes origin. Recommended applications/industries: Due to the health benefits and antioxidant compounds found in the essential oils of these plants are examined in vitro and in food. Manuscript profile
      • Open Access Article

        9 - Effect of different level water and nitrogen on morphological and physiological traits of Matricaria aurea L.
        فیروزه قانی دهکردی عبدالله قاسمی پیربلوطی بهزاد حامدی فاطمه ملک پور
        Background & Aim: Matricaria aure is one of medicinal plants belonging to Asteraceae family. They naturally grow in some regions of the country and have different medical properties and various applications in medicine production and traditional medications. Ecologi More
        Background & Aim: Matricaria aure is one of medicinal plants belonging to Asteraceae family. They naturally grow in some regions of the country and have different medical properties and various applications in medicine production and traditional medications. Ecological conditions have a significant impact on growth, performance, and quality of medical plants. The aim of this research is to investigate the effects of two important ecological factors (nitrogen and water) on the growth, performance and essential oil from Matricaria aure.Experimental: The present research has been carried out at the researches centre of medical plants in Shahrekord Branch, Islamic Azad University, in 2008. In this research, the factorial experiment with completely random bases and three replications were used. Five different levels of nitrogen (0, 500, 1000, 1500, and 2000 mg/pot) and three levels of irrigation (0, 25, and 50 % depletion of the soil moisture) were studied.Results & Discussion: The result of analysis of variance of the experiment for Matricaria aurea showed that different levels of nitrogen do have significant impacts on the plant height, chlorophyll content, total dry matter and % essential oil. Also, the impact of different levels of water on all characteristics was significant.Industrial and practical recommendations: The findings of this research indicated that the appropriate nutrition of nitrogen and not receiving drought stress can boost the essence production in Matricaria aure. Manuscript profile
      • Open Access Article

        10 - The essential oils of some medicinal plants on the immune system and growth of rainbow trout (Oncorhynchus mykiss)
        عبدالله قاسمی پیربلوطی اسماعیل پیرعلی غلام رضا پیشکار سید محمدعلی جلالی مهدی رئیسی محسن جعفریان دهکردی بهزاد حامدی
        Background & Aim: In present study, some of the Iranian endemic medicinal plants were used to evaluate potential as well as therapeutic agent and immunostimulants in aquaculture. The essential oils of five species from leaves and inflorescence were used to determina More
        Background & Aim: In present study, some of the Iranian endemic medicinal plants were used to evaluate potential as well as therapeutic agent and immunostimulants in aquaculture. The essential oils of five species from leaves and inflorescence were used to determinate the effects on immune system in rainbow trout (Oncorhynchus mykiss). Experimental: The experimental in a completely randomized design with 7 treatments and 3 replicates and each replicate fish was divided into 12 pieces. An experiment with 252 fishes with average weight 50±5 g for 8 weeks was conducted. The experimental treatments were: essential oil of 1. Satureja bachtiarica Bunge; 2. Thymus daenensis Celak; 3. Satureja Khuzestanica Jamzad; 4. Dracocephalum multicaule Benth; 5. Mentha longifolia L) 1% oil); 6. Normal diet + olive oil; 7. Normal diet (control). Results & Discussion: The results of analysis of variance showed significant different (p≤0.05) between almost of factors of immune system and growth of rainbow trout. The results showed the highest levels of immune factors relating to the percentage of phagocytosis and the number and rate of crime phagosite Igm three essential oils of M. longifolia, S. khuzestanica, and D. multicaule. Lymphocytes in the blood and blood heterophile between yourself and the control diet containing the essential oil of Satureja bachtiarica significant difference (p≤0.05) was observed. There was no significant difference between some of the factors of immune system and growth of rainbow trout (hematocrit, hemoglobin, monocytes and etc). Industrial and practical recommendations: The use of essential oils and herbs, especially the Mentha longifolia and Satureja bachtiarica can improve the status indicator. Manuscript profile
      • Open Access Article

        11 - Study the ethno-veterinary of medicinal plants in Kerman province, Iran
        عابد کوهپایه عبدالله قاسمی پیربلوطی محمد مهدی یزدان پناه راوری الهام پور محسنی نسب دل آرام ارجمند
        Background & Aim: The aim of this research is investigation and familiarity with the important herbal drugs that have used for traditional curing of animal diseases in different areas of Kerman province, Iran. From a long time ago native farmers and tribes of differ More
        Background & Aim: The aim of this research is investigation and familiarity with the important herbal drugs that have used for traditional curing of animal diseases in different areas of Kerman province, Iran. From a long time ago native farmers and tribes of different regions used the available natural herbs which existed in those regions to cure animal diseases in different areas of Kerman province. Experimental: In the present study, firstly the prepared questionnaires are used to gather information of traditional farmers and native tribes of the region regarding the use of herbs such as: when to use them, how to use them and the part of them which is used in the treatment of animal diseases and then the collection of natural herbs takes place by field search method. After that the collected herbs from different regions were dried and after the preparation of herbarium, they were transferred to Pharmacy School of Kerman University of Medical Sciences. Results & Discussion: After investigation, 77 types of herbs were finally gathered from different areas of Kerman province which were used in the treatment of animal diseases by farmers and native tribes. The information related to each of these plants (when to use, how to use, and the part of the plant which is used) is recorded in the questionnaires. The identified herbs in traditional medicine are used as Ajuga chamaecistus in removing the lice and skin parasites in goat and sheep and Cercis siliquastrum in treatment of deep and infectious wound in livestock and Dendrosllera lessertii in treatment of colic and bloating in sheep in the treatment of different domestic animals. Industrial and practical recommendations: The results of this study showed that with respect to the rich amount of fluor in the province of Kerman from the herbal point of view and the long history of farming in this province, more studies in this area can pave the way for the production of new herbal medicines to cure animal diseases. Manuscript profile
      • Open Access Article

        12 - Chemical Composition and Bioactivity of the Essential Oil of Melissa officinalis L., Cultivated in Southwestern, Iran
        هاله محمدپور عبدالله قاسمی پیربلوطی فاطمه ملک پور بهزاد حامدی
        Background & Aim: Melissa officinalis L. (lemon balm) a valuable medicinal plant in herbal medicine is native to the eastern Mediterranean region and western Asia. It is a traditional herbal medicine, which enjoys contemporary usage as a mild sedative, spasmolytic a More
        Background & Aim: Melissa officinalis L. (lemon balm) a valuable medicinal plant in herbal medicine is native to the eastern Mediterranean region and western Asia. It is a traditional herbal medicine, which enjoys contemporary usage as a mild sedative, spasmolytic and antibacterial agent. The objective of this study was to assess the effects of climate on quality and quantity of the essential oil of M. officinalis at Shahrekord climate, southwestern (Iran) and evaluate the antioxidant activity of the oil. Experimental: Plants were cultivated during 22-25th March 2016 in the greenhouse and transferred to the main site after three months (May), and finally harvested at the full flowering stage during August.Dried plant material (100 g) was powdered and subjected to hydro–distillation for three hours using a Clevenger–type apparatus. The hydrodistillated essential oil analyzed by GC-FID and GC/MS. The antioxidant activity was determined by using DPPH method. Results: Results indicated that the major essential oil constituents of M.officinalis L. were β-caryophyllene (23.06%), E-citral (17.61%), Z-citral (13.64%), and caryophyllene oxide (10.83%). The antioxidant activityof the essential oil showed moderate antioxidant activity (IC50=749.60 µg/g), that was lower compared to butylated hydroxytoluene (BHT). Recommended applications/industries: This potential applicability can be used as antioxidant agents for food and pharmaceutical industries. Manuscript profile
      • Open Access Article

        13 - Qualitative and quantitative changes in the essential oil of sage (Salvia hydrangea DC exBenth.) as affected by different drying methods
        فروغ مهدیان عبدالله قاسمی پیربلوطی فاطمه ملک پور
        Background & Aim: Salvia hydrangea is one of the medicinal plants belonging to Lamiaceae family. Medicinal plants can be marketed as fresh or dried products; however aromatic plants are often dried before extraction to reduce moisture content. The aim of this study More
        Background & Aim: Salvia hydrangea is one of the medicinal plants belonging to Lamiaceae family. Medicinal plants can be marketed as fresh or dried products; however aromatic plants are often dried before extraction to reduce moisture content. The aim of this study was to evaluate the impact of different drying methods on qualitative and quantitative changes in essential oil of sage. Experimental: To determine the effect of drying methods on qualitative and quantitative characteristics of the plant essential oil, samples were dried in sunlight, shade, mechanical oven at 65ºC and then compared with fresh samples. The essential oils of all samples were extracted by hydrodistillation and analyzed using GC and GC–MS. Results: The results showed that different drying methods had significant effects on essential oil content. According to results fresh herbs had the highest essential oil content followed by shade drying, sun drying and oven drying samples, respectively. Significant changes in chemical compound amount of the essential oils were observed which associated with the drying methods. Significant differences occurred among several constituents in the extracted essential oils, including a-pinene, camphene, phellandrene, 1,8-cineole and bornylacetate. Recommended applications/industries: Drying of Salvia hydrangea aerial parts in the shade is more suitable for obtaining higher amount of oil yield and percentage of a-pinene and 1,8-cineole. Therefore, shade drying could be recommended for extraction of essential oil from this plant on an industrial scale. Manuscript profile