List of Articles

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  1 - Assesment of different DNA extraction methods in medicinal plants
  مهدی رحیم ملک
  Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of D More

  Medicinal plants have high importance in medicine and pharmacogonosy because of their secondary metabolites. Essential oils, antioxidants and flavonoids are the major compounds of many medicinal plants. These compounds especially antioxidants can increase oxidation of DNA in response to wounds. Nowadays, progresses in biotechnology such as molecular markers were used for phylogenetic relationships, construction of linkage maps and decreasing the repeated plants in gene bank. Most of these methods need high DNA quality and purity. Different methods for DNA extraction have been improved. In most cases three hints are considered including quality, quickness of extraction and its quantity. Medicinal plants need to improve methods to prevent DNA oxidation because of their phenolic compounds. In this study, four DNA extraction methods were compared in thyme, spearmint, peppermint, horsemint and some yarrow species. These methods include Murry and Thompson, Prittila et al, Dellaporta et al and Komatsuda et al method using blender. The results showed that the combination of Prittila et al and Murry and Thompson methods using two steps buffer and using mixed of 2- Mercaptoethanol and Polyvinylpyrrolidone (PVP) can decrease DNA oxidation and improve DNA amount. The lowest amount of DNA and the quickest method was Komatsuda et al procedure. In the studied plants, the highest and the lowest oxidation were observed in yarrow and spearmint, respectively. Manuscript profile
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  2 - Interactive effects of salicylic acid and cold stress on activities of antioxidant enzymes in Glycyrrhiza glabra L.
  نرگس سلطانی دلربا رویا کرمیان مسعود رنجبر
  Background & Aim: Glycyrrhiza L. (Fabaceae) consists of perennial herbs grow in sandy soils with hard lightness. Three species of the genus grow in Iran, among them G. glabra L. has the widest distribution. It is well known in English as Licorice and the root extrac More

  Background & Aim: Glycyrrhiza L. (Fabaceae) consists of perennial herbs grow in sandy soils with hard lightness. Three species of the genus grow in Iran, among them G. glabra L. has the widest distribution. It is well known in English as Licorice and the root extract includes flavonoids and glycyrrhizin are used widely in medicine, food industry, tobacco products and other industries. Liquiritin and glabridin are the major constituents of flavonoids in its extract, which have quasi estrogenic, ant oxidative, anti-helicobacter, anti-nephritic activities and ROS scavenging.Experimental: In this research, the interactive effects of salicylic acid (SA) with cold stress on some antioxidant enzymes such as peroxidase (PRX) and polyphenol oxidase (PPO) were studied by spectrophotometric method in a factorial experiment of completely randomized design with three replications. SA pretreatments were included 10, 50, 100 and 500 µM and three levels of cold stress, 5, 10 and 20h in 4 oC were applied.Results & Discussion: The results showed that cold stress did not affect PPO activity, while SA decreased it. In addition, PRX activity was decreased with increasing cold stress and SA resulted in similar response at this condition. It seems that SA can considerably alleviate oxidative damage that occurred under cold stress condition with direct scavenging of reactive oxygen species. Thus it resulted in decrease activity of antioxidant enzymes. Manuscript profile
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  3 - The study of flora Ghaysari region in Chaharmahal va Bakhtiari province with 179 species of medicinal plants
  حمزه علی شیرمردی اصغر شاهرخی حسین محمدی نجف آبادی محمود طالبی
  Background & Aim: The present study was to identify the medicinal plant species and introduced as one of the key priority areas for conservation and determination of endangered species were done.Experimental: The method of plant collection in this region was classic More

  Background & Aim: The present study was to identify the medicinal plant species and introduced as one of the key priority areas for conservation and determination of endangered species were done.Experimental: The method of plant collection in this region was classical method of regional plant taxonomic studies. Collected plants were recognized and determined families, genera and species of them by using of indispensable references. The life form of plant species was determined by using of Raunkier’s method.Results & Discussion: The results of this study show that, 487 plant species belonging to 65 families and 301 genera exist in this area. Among the 487 species listed in this area, about 179 species (36.76% of the species) are medicinal. Furthermore, threatened species have been analyzed according to the IUCN criteria and Red Data Book of Iran. On the basis of this study, four categories of rare species so called endangered, vulnerable, lower risk and data deficient are determined. Accordingly, 86 threatened species were identified in this area that they all belong to the region, Iran – Tourna and 33 species that have some medicinal properties. Of these 86 species, two species in endangered class, three species in vulnerable class, 67 species in lower risk class and 14 species in data deficient class. Due to use nomads and villagers in the area of ​​medicinal plants, some species such as, Ferula assa-foetida, Ferula gumosa, Allium ampeloprasum, Allium jesdianum, Allium hirtifolium and Zaravschanica membranacea are subject to destruction. Manuscript profile
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  4 - Determine optimum of harvest time on the quantity/quality of essential oil and thymol of thyme (Thymus vulgaris L.) in Isfahan
  امین هادی پناه احمدرضا گل پرور عبدالله قاسمی پیربلوطی حسین زینلی
  Background & Aim: Thyme is common named Thymus vulgaris L. belongs to Lamiaceae family. Thyme is a most important medicinal plant that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed countries.Experimental: Determine optimum of h More

  Background & Aim: Thyme is common named Thymus vulgaris L. belongs to Lamiaceae family. Thyme is a most important medicinal plant that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed countries.Experimental: Determine optimum of harvest time this plant was done in a random block design with three replications was conducted in field Islamic Azad University of Khorasgan (Isfahan) 2009 and 2010. Treatment phonological stages such as: 1) before flowering stage; 2) at beginning of flowering stage; 3) 50% blooming stage; 4) at full flowering step and 5) at fruit set stage. The essential oil obtained by hydro-distillation using a Clevenger- type apparatus and, was analyzed by GC and GC/MS.Results & Discussion: Statistic analysis showed that the effects phonological stages had significant effect on essential oil yields & percent, thymol. The highest essential oil content (2.42%) was extracted at the beginning of flowering stage. Analysis and identification of components showed thymol main compounds in all samples.Industrial/Practical recommendation: According to the results of this project 50% blooming stage optimum of harvest time on the Quantity/Quality of Essence and thymol of Thyme. Manuscript profile
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  5 - The effect of different concentrations of IAA and NAA on the essential oil of Chaerophyllum aureum L.
  علیرضا فیض بخش فاطمه مکاوی پور
  Introduction & Aim: Plant growth regulators (PGR) have same effect as natural plant's hormones and can change the main components in plant samples. Chaerophyllum aureum L. is related to Apiaceae family.Experimental: In this paper the qualitative and quantitative com More

  Introduction & Aim: Plant growth regulators (PGR) have same effect as natural plant's hormones and can change the main components in plant samples. Chaerophyllum aureum L. is related to Apiaceae family.Experimental: In this paper the qualitative and quantitative composition of the volatile oils of ‎Chaerophyllum aureum L. were reported after using different concentrations of IAA and NAA ‎on aerial parts of the plant at flowering time. The essence was recovered by using ‎hydro-distillation with a Clevenger type apparatus and investigated by GC and GC/MS.‎Results & Discussion: The main components in the control sample are: α-pinene (14.1%), β-pinene (11.2%), ‎camphene (7.3%), verbenone (2.3%), limonene (14.4%), and trans-verbenol (3.7%). The results ‎indicated that IAA will result in the percentage increase of germacrene-D and, ‎of α-humulene (about 4%) and, decrease of α-pinene and β-pinene. NAA will result in the ‎production of β-bisabolene (2.5%), the increment of α-cadinol about 2 times, the diminution ‎of camphene (less than half) and of α-pinene and β-pinene too.‎ Manuscript profile
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  6 - Determination of alkaloids amount from Iranian Papaver bracteatum Lindl. by HPLC
  زهره کدخدا سجاد صداقت شمس علی رضازاده حسن علی نقدی بادی رحیم تقی زاد فرید ‏فرهاد ‏حریری اکبری
  Background & Aim: Papaver bracteatum Lindl. (Papaverace) is an important pharmaceutical plant which contains isoqinoline alkaloids. Isoqinoline alkaloids constitute one distinct group of secondary metabolites of the Papaverace family. Codeine and thebaine are import More

  Background & Aim: Papaver bracteatum Lindl. (Papaverace) is an important pharmaceutical plant which contains isoqinoline alkaloids. Isoqinoline alkaloids constitute one distinct group of secondary metabolites of the Papaverace family. Codeine and thebaine are important alkaloids of Papaver bracteatum which use widely due to its medicinal properties. Therefore it is necessary to determination the contents of these alkaloids in different parts of Iran. In order to determine the best natural habitat of Papaver bracteatum, containing the highest concentration of thebaine and codein different sample collected from different parts of Iran.Experimental: Using high performance liquid chromatography (HPLC) standard curve with the presence of two major alkaloids (thebaine and codeine) were drawn and alkaloids were extracted from capsule Papaver bracteatum and were determined by HPLC.Results & Discussion: Codeine contents in the sample of dry capsule Zanjan, Plour, Chalus road, Siah Bisheh, and Mazandaran were determined 0.04, 0.04, 0.03, 0.01 and 0.02% respectively. Thebaine content was determined 3.3, 0.61, 0.34, 0.31 and 0.06% respectively. Amounts of codeine for different regions ranged between 0.001 to 0.004% and, 0.06 to 3.3% for thebaine. The accessions collected from Zanjan has highest percentage of codeine and thebaine (3.34%)Industrial/Practical recommendation: The capsules collected from region of Zanjan in Iran have more contents of thebaine and codeine which possess many pharmaceutical applications, therefore it is highly recommended using accessions from the region of Zanjan to extract thebaine and codeine consumed in the pharmaceutical industry. Manuscript profile
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  7 - Effect of Boswellia serrata Triana & Planch. gum resin administration during lactation on morphology of pyramidal neurons in hippocampus of rat
  محمد حسینی شریف آباد ابراهیم اسفندیاری
  Background & Aim: In traditional medicine, Boswellia species gum resin known as Frankincense or Olibanum, has been administrated in elderly for enhancement of memory and also in pregnant women to increase memory and intelligence of progeny. However, it has been rare More

  Background & Aim: In traditional medicine, Boswellia species gum resin known as Frankincense or Olibanum, has been administrated in elderly for enhancement of memory and also in pregnant women to increase memory and intelligence of progeny. However, it has been rarely scientifically documented so far. We have previously reported that maternal administration of Frankincense during lactation increased learning and memory performance. In this study, using animal model of rat, we investigated the morphological changes of pyramidal neurons of hippocampus, an important brain centre involved in learning and memory, following maternal administration of Frankincense during three week of lactation. Experimental: Two months-old male Wistar rats whose mothers were given by gavage the aqueous extract of the B. serrata (0.100 mg/kg/day) during lactation were deeply anesthetized and transcardially perfused with fixative solution. Each brain was then removed from skull and divided into two hemispheres. The right hemispheres were selected for morphometerical study. Transverse sections (100 μm thick) were cut through the entire extent of the hippocampus. In a Golgi study, the number of dendritic branching and segments was counted by Sholl’s method. Results & Discussion: Statistical analysis of data indicated that administration of Frankincense during lactation increase the number of dendritic segments, branching of Cornu Ammonis (CA) pyramidal cells in the hippocampus of rat offspring. There was no significant difference in the dendritic length of CA pyramidal cells between experimental and control groups. These results suggest that maternal administration of Frankincense during lactation increase dendritic arborization in the hippocampus. It can be concluded that such morphological changes leads to an increase in the synaptic contacts and it provides one of the bases for the memory improvement induced by Frankincense administration. Industrial/Practical recommendation: Our findings suggest that Boswellia resin and its active ingredients to be extensively studied in neurophysiology. It also suggests that compounds from Boswellia resin may be used in treatment of neurodegenerative disorders. Manuscript profile
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  8 - Effect of hydroalcoholic extract of Nigella sativa L. on the immune system and blood protein components in mice
  مهرداد مدرسی
  Background: Black seed (Nigella sativa L.) in traditional medicine is well known that the chemical composition of the immune system with many applications in traditional medicine to fight the infection. This study investigated the effect of the plant's role in the immun More

  Background: Black seed (Nigella sativa L.) in traditional medicine is well known that the chemical composition of the immune system with many applications in traditional medicine to fight the infection. This study investigated the effect of the plant's role in the immune system and blood protein.Experimental: In this study male mice of Balb/c strain were divided into four groups tested. Control and experimental groups 1, 2 and 3, respectively, 50, 100 and 200 dose mg/Kg of the extract of Nigella sativa seed hydro alcoholic extract in 20 days and injected intraperitoneally received. At the end of the experiment, blood was taken for white cell count and blood protein electrophoresis was used. The data obtained with the SPSS software.Results and discussion: Studies suggest that the extracts of Nigella sativa in the groups are able to increase white blood cell count, protein and albumin, alpha 1 and 2 and the A/G.Industrial/Practical recommendation: According to the survey results appear in the pharmaceutical industry play an effective role in strengthening the immune system of Nigella sativa and, play in this context can be used as a medicinal plant use. Manuscript profile
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  9 - Comparison of antibacterial activity of Eucalyptus globulus Labill. and Malva neglecta Wallr.
  مجید دوست محمدی پیمان عبداله زاده حامد علیزاده
  Background & Aim: Very studies results show that many plants can be used safety and successfully to treat bacterial diseases without presenting the disadvantages affects and antibiotic resistance. Eucalyptus globulus and Malva neglecta are native plants in Iran that More

  Background & Aim: Very studies results show that many plants can be used safety and successfully to treat bacterial diseases without presenting the disadvantages affects and antibiotic resistance. Eucalyptus globulus and Malva neglecta are native plants in Iran that due to containing different chemical compounds have been antimicrobial activity against some bacteria. The aim of this study is comparison of antibacterial effects of Eucalyptus globulus and Malva neglecta extracts on Staphylococcus aureus, Enterococcus faecalis, Salmonella typhimurium and Pseudomonas aeruginosa.Experimental: In this study, at the first time aquatic, ethanolic, acetonic and chlorophormic extracts of E. globulus and M. neglecta dried leaves prepared. Then MIC and MBC of the extracts for the bacteria with micro-dilution method and well diffusion method performed for dosage depending effect.Results & Discussion: The results showed that both plant extracts have antimicrobial activity against bacteria, but antibacterial activity of various extracts of E. globulus is more than M. neglecta extracts and, this extracts can be used in the manufacture of new drugs of plant origin.Industrial/Practical recommendation: According to antimicrobial effects of Eucalyptus globulus and Malva neglecta extracts in this study recommended after human studies, it is prepared herbal drug from this plant and presented. Manuscript profile
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  10 - Effect of some of plant extracts on the growth of two Aspergillus species
  سیما یحیی آبادی الناز زیبانژاد منیر دودی
  Background & Aim: In recent years using from plant product has been considered wildly as main factor for preventing pathogenic diseases cassed by attacking viruses, bacteria, fungi and parasites. There are some funguses which could be the factors for affliction of s More

  Background & Aim: In recent years using from plant product has been considered wildly as main factor for preventing pathogenic diseases cassed by attacking viruses, bacteria, fungi and parasites. There are some funguses which could be the factors for affliction of some diseases in human, plants and animals. Plant extracts are those which could act and be used as preventing from these diseases as product of opposed fungus. The main aim of this article is considering the effects of Anethum graveolens, Thymus vulgaris, Coriandrum sativum and Rosa damascena is on standard strains and separated as Aspergillus flavus and Aspergillus fumigatus. Experimental: The sink was used for preventing the development of plant extract and not growing different density of extract were considered separately. The minimum inhibitory concentration (MIC) was calculated by the serial diluted method. Results & Discussion: The result showed that Aspergillus flavus standard (PTCC 5006), nystatin and extracts of A. graveolens, C. sativum and T. vulgaris in equal amount and finally R. damascena had more effects in opposed fungus. About separated strain of this fungus from the environment, nystatin, T. vulgaris, A. graveolens, C. sativum and finally R. damascena had the most effect against fungus. About standard Aspergillus fumigatus (PTCC 5009), the most effective mixtures against fungus were included extracts of A. graveolens, R. damascena, and C. sativum respectively. About separated strain of this fungus until the most effective mixture of opposed fungus respectively were including nystatin, A. graveolens, T. vulgaris, C. sativum and finally R. damascena. The result of this investigation shows that the existence extracts will be the main cause of reducing the growth of fungi. In this case by increasing the extracts of A. graveolens, T. vulgaris, C. sativum and R. damascena will be increased. Industrial/Practical recommendation: By proving the effect distributing the extracts leaves of Anethum graveolens, Thymus vulgaris, Coriandrum sativum and Rosa damascena on growing of two kinds of Aspergillus containing as Aspergillus flavus and Aspergillus fumigatus, there will be a hope that in future, by delivering the effective substances of above plants, and doing more experiment and investigation we would be able to reach an acceptable effects and having less side effect and complications for curing fungus infection diseases. Manuscript profile