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      • Open Access Article

        1 - The effect of ethanolic and aqueous extracts of Berberies vulgaris on multidrug- resistant gram-negative pathogenic bacteria
        زهرا عطائی کچوئی سیما یحیی آبادی منیر دودی
        Background and Aim: In recent years, due to the indiscriminate and irrational use of synthetic drugs, the resistance of pathogenic microorganisms has increased; therefore, new compounds are vitally needed. The purpose of this study was to investigate the effect of ethan More
        Background and Aim: In recent years, due to the indiscriminate and irrational use of synthetic drugs, the resistance of pathogenic microorganisms has increased; therefore, new compounds are vitally needed. The purpose of this study was to investigate the effect of ethanolic and aqueous extracts of Berberis vulgaris on Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Citrobacter frundi, Enterobacter aerugenes, Pseudomonas aeruginosa and Acinetobacter baumannii in standard conditions. Material and methods: Ethanolic and aqueous extracts of B. vulgaris were prepared by maceration method. After the bacteria were identified, antibiotic resistance was determined by agar disk diffusion method. Antibacterial effect of ethanolic and aqueous extracts of B. vulgaris on multidrug resistant bacteria was examined at four concentrations of 50, 100, 400 and 800 mg/ml, and (MIC) and (MBC) of these extracts on macro dilution method was also used. The collected data were analyzed using SPSS Software and Kruskal-Wallis and Mann-Whitney tests. Results and Conclusion: The results showed that ethanolic and aqueous extracts of B. vulgaris had an antibacterial effect on a multi-drug-resistant bacteria (MDR). The MIC and MBC of the extracts were reported 50 and 100 mg/ml, respectively. Industrial and practical recommendations: After further investigations, extract B. vulgaris was recommended to be utilized as an alternative to antibiotics for treatment. Manuscript profile
      • Open Access Article

        2 - Genetic improvement of quantity/quality yield of black cumin (Nigella sativa L.) ecotypes cultivated in Iran climatic conditions
        سعید صالحی اسد رخ زدی قربان نورمحمدی سید محمدجواد میرهادی احمدرضا گلپرور
        Background & Aim: Black seed or black cumin (Nigella sativa L.) belongs the family Ranunculaceae. Black seed is one of these species, which is naturally distributed in different parts of the country. It is extensively cultivated in various regions of Iran. Experime More
        Background & Aim: Black seed or black cumin (Nigella sativa L.) belongs the family Ranunculaceae. Black seed is one of these species, which is naturally distributed in different parts of the country. It is extensively cultivated in various regions of Iran. Experimental: The experiment was achieved using randomized complete block design with three replications in the research field of Islamic Azad University of Isfahan Branch during 2013. Seed of ten ecotypes namely; Semirom, Zawareh, Golpayegan, Fereydan, Meymeh, Kashan, Khansar, Daran, Ardestan and Isfahan were cultivated in the plots comprising four rows. The essential oil was extracted by a Clevenger type apparatus andanalyzed by using GC/MS. Results & Discussion: Statistical analysis indicated that there was significant difference among all traits. The results of regression and path analysis indicated that the number of branches, grain filling rate and days to blooming traits were the best indirect selection criteria to improve yield in black cumin. According to the results of this research, Fereydan and Daran ecotypes showed the optimum amount of essential oil content andmorphological traits. Furthermore, cross between these ecotypes has suitable advantage to increase seed and oil yield in black cumin breeding programs. Industrial and practical recommendations: There are few researches about breeding of black cumin. The results of present study could be applied in research centers as well by farmers to cultivate commercially and production black cumin especially for seed. Manuscript profile
      • Open Access Article

        3 - Identification and characterization of chemical composition of Rhus coriaria L. fruit from Hamadan, Western Iran
        حمیدرضا اردلانی مریم حسن پور مقدم امین هادی پناه فرنوش فتوت علی عزیزی جلال سلطانی
        Background & Aim: Identification, characterization and detection of medicinal plants chemical composition are very important for phytochemical scientific source. Sumac is one of the medicinal plants in the world that uses for industrial and pharmacological applicati More
        Background & Aim: Identification, characterization and detection of medicinal plants chemical composition are very important for phytochemical scientific source. Sumac is one of the medicinal plants in the world that uses for industrial and pharmacological application. In this study, chemical composition of Rhus coriaria has been investigated. Experimental: Chemical composition of the mature and ripe fruit of Rhus coriaria cultivated in Hamedan (Iran) climatic conditions has been analyzed by high performance liquid chromatography–electrospray ionization mass spectrometry (HPLC-MS). Results & Discussion: In total 191 compounds were identified in sumac fruit including, 78 hydrolysable tannins (Gallic acid), 59 flavonoid such as, Apigenin, 40 other compounds such as Butein, 9 anthocyanins such as Cyanidin. Industrial and practical recommendations: The results indicated that this plant is one of the extensive sources of bioactive phytochemical, phenolic compounds and as a source of functional ingredients in chemical, pharmaceutical industries, traditional medicine and herbal drugs as widespread uses in the world. Manuscript profile
      • Open Access Article

        4 - Regional variations of antioxidant capacity and phenolic properties in the Iranian jujube collection
        علی عزیزی مریم پیربداقی
        Background & Aim: Fruits from the jujube plant (Ziziphus jujuba) possess health-promoting effects and medicinal properties. Jujube has a long history of usage as a remedy in Iranian traditional medicine and is recommended for the treatment of some diseases. This stu More
        Background & Aim: Fruits from the jujube plant (Ziziphus jujuba) possess health-promoting effects and medicinal properties. Jujube has a long history of usage as a remedy in Iranian traditional medicine and is recommended for the treatment of some diseases. This study investigated the variations in antioxidant capacities (AOX), total phenol (TPH), total flavonoid (TF) and total anthocyanin (TA) contents of fruit extracts among 29 jujube accessions originating from 7 provinces of Iran. Experimental: The fruits from 29 accessions were extracted with methanol (85%). DPPH assay were performed for determination of antioxidant capacity of each extract. The total phenols were measured by Folin-Ciocalteu method and total flavonoids evaluated using the aluminum chloride colorimetric procedure while total anthocyanins were determined by pH-differential method. Results & Discussion: The results from the analysis of variance (ANOVA) revealed that the examined accessions of Z. jujuba were highly variable in all evaluated phytochemical traits (P <0.01). Principal component analysis (PCA) based on four phytochemical characters confirmed this broad variation and also identified three distinct group among the accessions. The accessions Hamidabad (Mazandaran), Bayaziyeh (Isfahan) and kuhpayeh2 (Isfahan) can considered by their high values for the TPH, TF, and TA values, respectively. On the other hand, accessions Ardestan (Isfahan), Ghaziolya (Ghom) and Maroon (Ghom) showed high AOX. No correlation was found between total phenol content and antioxidant activity. Industrial and practical recommendations: These findings suggest the importance of genotype and origin for determining antioxidant potential and phenolic contents of jujube plants. The knowledge of the diversities, found in this study, will allow a better plant exploitation related to pharmaceutical uses and also a better conduction of breeding programs for Ziziphus jujubaecotypes. Manuscript profile
      • Open Access Article

        5 - Chemical composition and antibacterial activity of essential oil from two Thymus species
        امین هادی پناه مجتبی خرمی
        Background & Aim: Staphylococcus aureus is one of the bacterial pathogens and the most important factor in hospitalized infections in human community. This bacterium is becoming resistance to chemical drugs quickly. Lots of countries are trying to find new compositi More
        Background & Aim: Staphylococcus aureus is one of the bacterial pathogens and the most important factor in hospitalized infections in human community. This bacterium is becoming resistance to chemical drugs quickly. Lots of countries are trying to find new compositions as a suitable replacement for these antibiotics. The aim of this study was antibacterial activity and chemical composition of (Thymus vulgaris L.) and (Thymus daenensis Celak) cultivated in Iran. Experimental: The aerial parts of T. vulgaris and T. daenensis were collected from stages beginning of blooming set at Estahban (Fars province) in South Iran, during 2014.The essential oil was extracted by a Clevenger approach and analyzed using GC/MS. This essential oil was examined on S. aureus with different sensitivity levels to vancomycin, methicilin and oxacillin antibiotics. Results & Discussion: In T. vulgaris and T. daenensisoil, 28, 26 compounds were identified, respectively. The major constituents of the T. vulgaris oil were thymol (63.14%), p-cymene (9.45%), g-terpinene (8.67%) and carvacrol (3.14%).The major constituents of the T. daenensis oil were; thymol (71.2%), g-terpinene (8.81%), p-cymene (3.8%) and carvacrol (2.4%). According to these results, MIC and MBC of T. vulgaris and T. Daenensis oil on S. aureus were estimated 0.1 to 0.5 micro l/ml respectively. Industrial and practical recommendations: In conclusion, the results obtained in our study indicated that Thymus daenensis oil had a high suppressing and fatal effects. Manuscript profile
      • Open Access Article

        6 - Recognition of Sulfur Compounds in Tissue Culture Different Organs of Persian Shallot (Allium hirtifolium Boiss) by GC/MS
        مهتاب اصفهانی زاده حسین پور فروغ مرتضایی نژاد
        Background & Aim: Persian shallot (Allium hirtifolium Boiss) with common name of "Musir" is one of the endemic medicinal plants in Iran. Leaves and bulbs with antibacterial, antifungal, anticancer, anti-diabetes and anti-obesity effects are edible parts of this plan More
        Background & Aim: Persian shallot (Allium hirtifolium Boiss) with common name of "Musir" is one of the endemic medicinal plants in Iran. Leaves and bulbs with antibacterial, antifungal, anticancer, anti-diabetes and anti-obesity effects are edible parts of this plant. The tissue culture is an influential method to multiply plant productions in order to meet demands of pharmacy and food industries. Therefore this article will focus on levels and structures of sulfur compounds in seeds, grassland bulbs and compare them with those of leaves, roots, bulbs and callus obtained from the tissue culture using the headspace- Gas chromatography-Mass Spectrophotometry (GC/MS) method. Experimental: At the first of the study, seeds and stem-discs of grassland bulbs were cultured in MS media. After 90 days seeds, grassland bulbs, seed-leaves, seed-bulbs, leaves and roots of seed-bulbs, stem-disc bulbs, and callus of stem-discs analyzed by GC/MS-headspace method. Results & Discussion: The results of analysis showed the highest variety of sulfur compounds was in seed bulb roots, while lowest levels were found in seeds, seed bulb leaves and callus. And also the highest level of sulfur compounds was discovered in stem-disc bulbs (28.31%), seed bulb roots (18.61%) and grassland bulbs (6.23%). Industrial and practical recommendations: Our findings has been shown Persian shallot sulfur compounds have mono, di and tri sulfur in structures and the best source for these metabolites were roots and stem-disc bulbs producing by in vitro culture method. Manuscript profile
      • Open Access Article

        7 - Chemical compositions of the essential oil of Gundelia tournefortii L. (Asteraceae) from Central Zagros, Iran
        حمیدرضا فرهنگ محمدرضا وهابی علیرضا علافچیان
        Background & Aim: Gundelia tournefortii L. (Tumbleweed) has been identified as one of the well-known and widely distributed plants with various applications in some of Asian and North African countries. It belongs to the (Asteraceae) family and grows in the Middle E More
        Background & Aim: Gundelia tournefortii L. (Tumbleweed) has been identified as one of the well-known and widely distributed plants with various applications in some of Asian and North African countries. It belongs to the (Asteraceae) family and grows in the Middle East particularly in the temperate, mountainous and semi desert areas of countries such as Iran, Iraq, Turkey, Jordan, Syria, Egypt, Turkmenistan and some regions of Azerbaijan and Armenia. The aim of this study was to identify of the chemical components of G.Tournefortii L. in some of its habitats into Central Zagros area, Iran. Experimental: In this regard, G. tournefortii L. was collected from some of its natural habitats in the mentioned area and air dried. The essential oil of the plant was isolated by hydro-distillation with a yield of 0.8 % (v/w). Also, the chemical compositions of volatile oil were analyzed using GC-MS. Results & Discussion: The results indicated that major components were palmitic acid (12.48 %), lauric acid (10.59 %), alpha ionene (6.68 %), myristic acid (4.45 %), 1-hexadecanol, 2-methyl (3.61 %), phytol (3.6 %), and beta turmerone (3.4 %). Industrial and practical recommendations: Gundelia tournefortii L. is known as a plants with many therapeutic properties especially in traditional and contemporary medicine in the world. Also, this plant has a useful nutritional solute such as potassium and calcium. Manuscript profile
      • Open Access Article

        8 - Study of qualitative characteristics of saffron cultivated in different regions of Iran
        نسیبه شریفی محمد حجت الاسلامی مریم جعفری
        Background & Aim: Saffron is one of the expensive spices known since antiquity for its color, flavor and medicinal properties. Saffron is the dried stigma of crocus sativus plant. The three basic components of the stigma on which the qualitative characteristics of S More
        Background & Aim: Saffron is one of the expensive spices known since antiquity for its color, flavor and medicinal properties. Saffron is the dried stigma of crocus sativus plant. The three basic components of the stigma on which the qualitative characteristics of Saffron depend are crocin (color), picrocrocin (taste), and safranal (aroma). The quality of saffron is a function of climatic conditions, soil type, as well as method of drying, packaging, and storage. Experimental: In this research, the saffron growing in different regions of Iran including Kerman, Shiraz, Arak, Natanz, Ghaen, Shahrekord, and Dolatabad has been studied with regard to its humidity, ash, phenolic compounds, crocin, safranal, and picrocrocin for its classification. Results & Discussion: The saffron of Shiraz and that of Kerman showed the maximum and minimum amount for ash with a value of 8.02% and 5.38%, respectively. With a view to moisture content, Natanz and Shahrekord saffron showed the highest and the lowest moisture content with a value of 11.97% and 9.7%, respectively. The phenolic compounds were determined by spectrophotometry and ranged from 17.43 mg gallic acid/g dry weight of saffron in Kerman sample to 8.87mg gallic acid/g dry weight in Dolatabad saffron. The highest level of safranal, crocin, and picrocrocin belonged to Shahrekord, Dolatabad, and Shahrekord saffron with a rate of 84.43, 234.4 and 38.4 (strength/ g dry matter) based on the most absorption over three wavelengths of 257, 440, and 330nm, respectively. Industrial and practical recommendations: With regard to variable applications of saffron as flavoring and coloring in food industry, the results of this study can be used for appropriate usage of saffron cultivated in different regions according to their specifications. Manuscript profile