List of Articles

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  1 - Pharmacognostic study, chemical analysis and antioxidant potential of Leucas indica L. (R. Br.)
  جاشری جاسوتکار راپالی شیرسات دیپاک کوچی
  Background & Aim: Leucas indica L. (R. Br.) is a lesser known aromatic herb of family Lamiaceae. Traditionally it was use to cure cough and cold by tribals of Southern India. The present work was aimed to investigate its pharmacognostic characters, major phytochemic More

  Background & Aim: Leucas indica L. (R. Br.) is a lesser known aromatic herb of family Lamiaceae. Traditionally it was use to cure cough and cold by tribals of Southern India. The present work was aimed to investigate its pharmacognostic characters, major phytochemicals and antioxidant potential as possible replacement of its allied members to reduce their exploitation. Experimental: The fine details of stem and leaves of L. indica were noted including vasculature, cortical and epidermal depositions, trichome structure and stomata. The fluorescence analysis of leaf powder was done using various laboratory chemicals and reagents. The methanol leaf extract was analyzed using HPTLC to identify major active compounds and antioxidant potential was studied using standard method. Results: Both simple and glandular trichomes were present on stem as well as leaves. The fluorescence analysis of powdered drug material after interaction with different laboratory chemicals showed distinct colorations. The preliminary analysis revealed the phytochemical richness of the plant. Most phytoconstituents were found to be extracted in methanol solvent. The HPTLC analysis of methanol extract showed presence of a range of phenolics and flavonoids. The chromatogram showed availability of rutin, ferulic acid, catechin and apigenin which makes this plant as a possible antioxidant drug candidate. Recommended applications/industries: The rich chemical diversity of this plant and significant antioxidant potential could be used as good natural source for herbal pharmaceuticals. Manuscript profile
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  2 - Cytogenetic activity of methanolic extract of aerial parts of Plumbago europaea on Balb/C Mouse bone marrow cells
  احمد خلیل حنان بشاره احمد ال-اوکلاه
  Background & Aim: The World Health Organization (WHO) estimated that majority of the inhabitants of the world rely chiefly on folk medicine. It, therefore, approved the use of herbal products for national policies and drug regulatory measures in order to strengthen More

  Background & Aim: The World Health Organization (WHO) estimated that majority of the inhabitants of the world rely chiefly on folk medicine. It, therefore, approved the use of herbal products for national policies and drug regulatory measures in order to strengthen research and evaluation of the safety and efficacy of herbal products. We have evaluated the cytotoxic, mutagenic and antimutagenic potential of the methanolic extract of the aerial parts of P. europaea, a common species in the Mediterranean and Central Asia. Experimental: Male Balb/C mice were intraperitoneally (i. v.) injected with varying doses of the extract dissolved in dimethyl sulfoxide (DMSO). The i.v. LD50 of the extract was determined to be 58.33mg/kg body weight. Bone marrow cells were processed and screened for chromosomal aberration and micronucleus formation. Mitomycin C (MMC, 2mg/kg b. w.) and DMSO (0.5%) served as positive and negative control, respectively. Antimutagenecity was followed by administration of 2mg MMC/kg in the beginning of the first or the last 24h of applying 14.6mg/kg of the extract daily for 7 consecutive days. Results: The selected doses of the extract elevated mitotic index and increased percentage of aberrant cells compared to the negative control. However, at a dose of 14.6mg/kg, the extract was enough to reduce significantly the toxic effects induced by MMC. This indicated that the P.europaea extract by itself is mutagenic, but antimutatgenic probably at small doses and can modulate the mutagenicity of MMC. Recommended applications/ industries: These data may help in understanding of therapeutic properties of P. europaea claimed by folk medicine. However, caution regarding indiscriminate use of this plant by the public is necessary. Multiple experiences are needed to rule out any possible side effects and to prove health safety of this natural product before introducing it into the market for therapeutic purposes. Manuscript profile
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  3 - Evaluation of the immunological changes in the use of Sumac herb powder (Rhuscoriaria) compared with Levamisole in dogs
  علی وحید دستجردی سام ترکان محسن جعفریان
  Background & Aim: One of the major problems of modern medicine is the increasing consumption of synthetic drugs and frequent use of them will result in side effects. Considering this issue, patients should be mainly motivated to use pharmaceutical plants. Regarding More

  Background & Aim: One of the major problems of modern medicine is the increasing consumption of synthetic drugs and frequent use of them will result in side effects. Considering this issue, patients should be mainly motivated to use pharmaceutical plants. Regarding the positive effects of sumac on the immune system, in the present study, we made a decision to compare sumac with levamisole as a chemical immune system stimulant compound. Experimental: In this research, 8 native breed of dogs were studied for ten days. The dogs were divided into four groups of two including a control group, a group consuming levamisole, a group consuming the amount of 10 mg per kg sumac, and a group receiving 5 mg per kg sumac. Then, blood samples were taken and the relevant tests were performed after completing the period of intaking the prescribed amount of powders of sumac plant and oral levamisole. Results: According to present results, significant differences were observed between treatment and control groups in terms of some factors including phagocytosis, multiplicity of phagocytosis mass and IGM whereas there was no significant difference in other factors (p < 0.05). It was revealed that sumac plant same as levamisole can increase immunity and phagocytosis in dogs. Recommended applications/ industries: In present study, considering the significant positive effects of sumac on some immunological parameters, this herb is recommended to be prescribed in the food. Manuscript profile
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  4 - The effect of Satureja bachtiarica on IL-6 and TNF-α in rat treated with Thioacetamide
  فاطمه ابراهیمی حسین سازگار
  Background & Aim: Acute liver damage, inflammation and oxidative stress are underlying tissue necrosis and development of ciroces. The aim of this study was to evaluate the effect of Satureja bakhtiarica extract on acute liver disease induced by the thioacetamide an More

  Background & Aim: Acute liver damage, inflammation and oxidative stress are underlying tissue necrosis and development of ciroces. The aim of this study was to evaluate the effect of Satureja bakhtiarica extract on acute liver disease induced by the thioacetamide and TNF-α and IL-6 cytokines level. Experimental: In experimental study, 36 Wistar rats were selected and divided into 6 groups of 6. The amount of 0.03g thioacetamide dissolved in 1ml of distilled water and injected intraperitoneally to all mice except of control group twice a week and for a period of three weeks. Negative control group received only thioacetamide and the other group received 8mg/kg sibilin by gavage in addition to thioacetamide. After injection of thioacetamide, the experimental groups were treated with doses of 5, 10 and 20 mg/ml extracts of S. bakhtiaricafor two weeks. Peripheral blood samples were taken from rat hearts. Then TNF-α and IL-6 cytokines levels were measured by Elisa method. Histopathological changes of liver also were examined. Data analysis was performed with SPSS ver. 20. Results: The results showed a significant difference in TNF-α and IL-6 cytokines between groups (p<0.001). Mean concentration of TNF-α and IL-6 in the groups treated with the doses of 5(P = 0.002, P <0.001), 10 (P= 0.010, P <0.001) and 20 mg/ ml (P <0.001, P <0.001) of S. bakhtiaricaca significantly decreased compared with group treated with thioacetamide. Histopathological results also supported dose dependent protective effect of S. bakhtiaricaca. S. bakhtiaricacaextract has anti-inflammatory properties that can reduce the toxicity of thiouzamide by reducing the levels of TNF and IL-6 cytokines as pro-inflammatory cytokines. Recommended applications/industries:Regarding that the use of herbal medicines to treat many diseases are on the rise, hydroalcholic extract of Satureja bachtiarica due to having antioxidant properties can be used to treat liver disease with drug therapy. Manuscript profile
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  5 - Antimicrobial activity of Prosopis farcta L. and Datura stramonium L. extracts against Staphylococcus aureus
  سعیده سعیدی نفیسه مهدی نژاد مجید شریفی راد فرشته جوادیان الهام جوادیان
  Background & Aim: The aim of this study was to investigate the antimicrobial activity of two rangeland-medical plants extracts, Prosopis farcta L. and Datura stramonium L., against Staphylococcus aureus isolated from sheep in Zabol city. Experimental: The P. farcta More

  Background & Aim: The aim of this study was to investigate the antimicrobial activity of two rangeland-medical plants extracts, Prosopis farcta L. and Datura stramonium L., against Staphylococcus aureus isolated from sheep in Zabol city. Experimental: The P. farcta and D. stramonium were collected from the rangelands of Zabol city. Staphylococcus aureus strains were isolated from the nose of sheep in Zabol city. Antibiotic resistance pattern was determined by Kirby Bauer method. Finally, the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) were determined by microdilution method. Results: The results of this study showed that the P.farcta extract had a MIC of 25 ppm, and 10 strains of the bacterium were inhibited in this concentration. The lowest inhibitory concentration of D.stramonium extract was 6.25 ppm, and one strain was inhibited in this concentration. On the other hand, the highest inhibitory concentration was 50 ppm and the highest bactericide concentration was 100 ppm. Recommended applications/ industries: The results of this study showed good antimicrobial effects of D. stramonium and P. farcta extracts that can be used to treat Staphylococcus aureus infections. Manuscript profile
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  6 - Evaluation of the antibacterial activity of different parts of Foeniculum vulgare (fennel) extracts
  آذین غفاری زاده سید منصور سیدنژاد حسین معتمدی فاطمه شهبازی
  Background & Aim: Foeniculum vulgare Mill. (Apiaceae) is a medicinal and aromatic plant. Various pharmacological experiments in vitro and in vivo models have convincingly showed F. vulgare ability in antifungal, antibacterial, antioxidant, antithrombotic and hepatop More

  Background & Aim: Foeniculum vulgare Mill. (Apiaceae) is a medicinal and aromatic plant. Various pharmacological experiments in vitro and in vivo models have convincingly showed F. vulgare ability in antifungal, antibacterial, antioxidant, antithrombotic and hepatoprotective activities. Experimental: The purpose of this study was to evaluate the antibacterial activity of extracts of Foeniculum vulgare on the number of pathogen bacteria. For this purpose, ethanol, methanol and acetone extracts (through maceration method) of different parts of the Foeniculum vulgare (flowers, leaves and fruit) were prepared and antibacterial activity was tested against some gram positive (Staphylococcus aureus, Bacillus cereus) and gram negative (Pseudomonas aeruginosa, Escherichia coli) bacterial species through standard disc diffusion method. Results: The findings showed that the most sensitive bacteria to the extracts was Staphylococcus aureus and the most resistant bacteria was Pseudomonas aeruginosa. According to the gained results in the case of fennel flower and fruits extract, methanol and acetone extract showed the highest and lowest antibacterial activity, respectively, while in the case of fennel leaves ethanolic extract exhibited the most inhibitory effect. In general, the results of this study indicated that the extract of fennel flowers had more antibacterial properties in comparison with other parts of plant. Recommended applications/ industries: The antibacterial activity of fennel is due to the presence of terpenoids and phenolic compounds. These compounds are potentially effective on membrane permeability of bacteria and thereby cause the cell death. Therefore, fennel extract can be used to control bacteria resistant to several antibiotics. Manuscript profile
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  7 - A comparative study of chemical composition and antibacterial properties of essential oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) from northern Khorasan Province
  علی فیروزنیا فهیمه دوست زاده نرگس ربیعی
  Background & Aim: Tanacetum is a member of Asteraceae family and its twelve species such as Tanacetum walteri are endemic to Iran. In this investigation, chemical compounds and antimicrobial activity of essential oils of Tanacetum turcomanicum (Krash.) and Tanacetum More

  Background & Aim: Tanacetum is a member of Asteraceae family and its twelve species such as Tanacetum walteri are endemic to Iran. In this investigation, chemical compounds and antimicrobial activity of essential oils of Tanacetum turcomanicum (Krash.) and Tanacetum walteri (C.Winkl.) were studied. Experimental: The plants were collected from north Khorasan province of Iran, in spring of 2016. The essential oils were extracted by the hydrodistillation method using clevenger. Identification of the essential oils chemical composition was conducted by GC/MS instrument. Also, Agar well diffusion method was used to investigate its antibacterial effects against E. coli, S. aureus, B. atrophaeus and M. luteus. Results: Essential oil efficiency of T. walteri species was obtained 0.2% v/w. In total 92.84 percent of essence constituents of 43 components were recognized in which α-pinene (22.37%), 1,8-cineole (12.52%), camphor (11.91%) and ß-thujone (4.52%) were recognized as the main constituents. By isolating essence of T. turcomanicum species, 39 constituents of 89.28% of the whole essential oils were recognized with efficiency of 0.3% v/w. Davanone (20.79%), 1,8-cineole (15.63%), p-cymene (10.20%), camphor (10.14%) and borneol (4.95%) were the main components of the essence. The highest antimicrobial activity of the essential oils of T. turcomanicum and T. walteri were observed against M. luteus, B. Atrophaeus and S. aureus, respectively. The essential oil of T.turcumanicum did not show significant activity against E. coli bacterium. Recommended applications/industries: The results showed the environmental influences and the effect of plant species on the amount and type of volatile components of essential oils. Also, antibacterial efficacy shown by these oils provides a scientific basis and thus validates their use as medicinal remedies. Isolation and purification of different phytochemicals may yield to significant antibacterial agents. Manuscript profile
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  8 - A systematic review and Meta-Analysis on the effect of Cotoneaster manna on Neonatal Jaundice
  علیرضا صالحی محدثه استوار مریم مرزبان
  Background & Aim: The manna of Cotoneaster nummularia (purgative manna) has been traditionally used in the Persian medicine for the treatment of neonatal jaundice. The effect of Cotoneaster manna on neonatal jaundice was investigated with systematic review and meta- More

  Background & Aim: The manna of Cotoneaster nummularia (purgative manna) has been traditionally used in the Persian medicine for the treatment of neonatal jaundice. The effect of Cotoneaster manna on neonatal jaundice was investigated with systematic review and meta-analysis. Experimental: This study intended to explore the clinical trials of the efficacy of Cotoneaster manna on neonatal jaundice. The aspects considered in this study included searching for relevant English and Persian data bases up to March 2016. The methodological quality was assessed using the Consort checklist for herbal interventions. Seven studies with low or moderate risk of bias involved 804 participants of which 410 cases versus 394 controls were selected for systematic review and meta-analysis with CMA software version 2.2. Results: Total plasma bilirubin in 0, 12, 24, 36, 48 hours and the duration of hospital stay were checked and no adverse effect was reported. The Meta-analysis exhibited marked heterogeneity of the results (Q-value = 132.446, P< 0.0001, I2 = 87.165, τ = 0.998). However, it showed favorable effect of Cotoneaster manna on reducing neonatal jaundice (n= 804, OR = 0.242, 95% CI: 0.147 to 0.399, P< 0.0001). Also it was effective on reducing duration of hospital stay (n= 804, SMD= -1.154, 95% CI: -1.854 to -0.455, P< 0.001). However, the results obtained were heterogeneous (Q-value= 119.642, P< 0.0001, I2= 94.985, τ= 0.913). Recommended applications/ industries: Although the meta-analysis of Cotoneaster manna showed positive effect on treatment of neonatal jaundice in these studies, it is warranted to carry out further multi-centre randomized clinical trials with larger samples and controlled risk factors, alongside comparison with phototherapy. Manuscript profile