List of Articles

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  1 - Effect of explants and growth regulators on direct organogenesis of Dracocephalum kotschyi Boiss. via tissue culture technique
  محمود اطرشی کوثر مرادی
  Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditi More

  Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct organogenesis of Dracocephalum kotschyi using tissue culture technique. Experimental: Various concentrations of BAP, IBA and NAA belong to auxin and cytokinin, as two important plant growth regulators (PGR), with shoot tip explants; hypocotyl and cotyledon were investigated in plant micropropagation of Dracocephalum kotschyi. Results & Discussion: Results indicated that the best treatment and explants on regeneration Dracocephalum kotschyi was MS medium containing 5 mg/L BAP, and 0.2 mg/L NAA on shoot tip explants. Regenerated explants on MS medium containing 1 mg/L BAP and 0.5 mg/L IBA had elongation growth and the elongation growth medium, plantlets were rooted. Rooted plantlets were then transferred to the hardening. After consistency of the regeneration plant seedlings Dracocephalum kotschyi, about 95% of seedling transferred to greenhouse suitable growing. Industrial and practical recommendations: According to the results of this research, we can start to proliferate and produce the effective matter of medicinal plant, such as Dracocephalum kotschyi. The most important benefit of this method is to prevent from extinction and to protect the species out of the environment. Manuscript profile
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  2 - Effect of different distillation methods on quantity and quality of essential oil of two Ziziphora L. species
  حسین بتولی مریم اخباری سید محمد جواد حسینی‌زاده
  Background & Aim: Ziziphora L. genus belongs to the Lamiaceae family, which has important medicinal and aromatic plant species. In current study, essential oil chemical compositions of two Ziziphora L. species (Z. tenuior L. and Z. clinopodioides Lam.) were investig More

  Background & Aim: Ziziphora L. genus belongs to the Lamiaceae family, which has important medicinal and aromatic plant species. In current study, essential oil chemical compositions of two Ziziphora L. species (Z. tenuior L. and Z. clinopodioides Lam.) were investigated. Experimental: The flowering branches of Z. tenuior and Z. clinopodioides were collected in spring and summer 2011. The aerial parts dried in shade at room temperature. The flowering branches of these species subjected to volatile fraction were isolated by simultaneous distillation extraction (SDE), hydro distillation (using a Clevenger-type apparatus), steam-cooled distillation (ultrasonic) and steam distillation. The analysis of the oils was performed by using GC and GC-MS. Results & Discussion: The results indicated the essential oil yield of Z. tenuior ranged 0.2% to 2.3% (w/w), and Z. clinopodioides ranged 0.3% to 1.3% (w/w(. In total, 18 components were identified in Z. tenuior essential oil, and 33 components were identified in Z. clinopodioides essential oil. The main components of Z. tenuior essential oil were pulegon (80.01% - 90.10%), and piperitenone (4.5% - 7.14%). The major compounds in Z. clinopodioides essential oil were pulegon (25.87% - 35.20%), piperitenone (10.11% - 27.88%), menthol (11.41%-17.50%), and menthon (0 - 7.69%). The highest percentage of menthol in essential oil in two species obtained from SDE method. Industrial and practical recommendations: According to the purity 81% of pulegon combination in essential oil of Ziziphora with 2% essential oil yield, can be purified in production of Ziziphora. Manuscript profile
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  3 - Ethnobotanical study of medicinal plants in Natanz region (Kashan), Iran
  شبنم عباسی سعید افشارزاده عبدالرضا مهاجری
  Background & Aim: Iran has a long history in ethnobotany and ethnopharmacology. The indigenous people in YahyaAbad area, is located in the conserved area of the Karkas mountains, are used many plants and natural material for treatment their diseases. YahyaAbad area More

  Background & Aim: Iran has a long history in ethnobotany and ethnopharmacology. The indigenous people in YahyaAbad area, is located in the conserved area of the Karkas mountains, are used many plants and natural material for treatment their diseases. YahyaAbad area is an important part of central mountains of Kashan, Central Iran. There are many plant species in this area which have medicinal uses. The aim of this research was ethnobotanical study on medicinal herbs of YahyaAbad area in order to recognize custom, tradition, and application way of plants by the people.Experimental: In current study, plant species were collected at several times. Plant identities were confirmed by botanist and references. An analysis was made of the species used, parts of the plant employed, preparation methods, administration means, and the ailments treated in relation to pathological groups.Results & Discussion: In total, 65 medicinal plant species collected and identified in YahyaAbad area. Results indicated some of medicinal plants are used commonly by the indigenous people. Many of medicinal plants are used for eliminating different pains. Generally, some of the uses were found to be new when compared with published literature on ethnomedicine of Iran. Industrial and practical recommendations: According to medicinal value of some plants in studied area, and non suitable climate condition such as droughts which caused to intensify livestock's grazing and irregular plants harvest, it seems using adopting the appropriate methods and conservation policies, we can prevent from this issue. Manuscript profile
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  4 - Essential fatty acids extracted from Portulaca oleracea L. leaves
  ژیلا اصغری سهره علی محمد زاده محسن مظاهری تهرانی
  Background & Aim: Purslane (Portulaca oleracea L.), belongs to the Portulacaceace family, grows in many parts of Iran. Results of previous studies were indicated purslane is a rich source of essential fatty acids of omega-3 and omega-6. Portulaca oleracea is a wides More

  Background & Aim: Purslane (Portulaca oleracea L.), belongs to the Portulacaceace family, grows in many parts of Iran. Results of previous studies were indicated purslane is a rich source of essential fatty acids of omega-3 and omega-6. Portulaca oleracea is a widespread weed, being the eighth common plants of the world. The Aim of current study was to compare the essential fatty acids composition in two populations of Portulaca oleracea from Gorgan and Sardasht regions.Experimental: The oil of purslane leaves was extracted by Soxhlent apparatus using petroleum ether. Extracted oil was change into methyl ester, before analysis by gas chromatography.Results & Discussion: The total fat contents of Gorgan and Sardasht populations were 4.71% and 4.81%, respectively. In both populations, three the essential fatty acids were detected. Linolenic acid, ranged from 105.43 to 148.97 mg FA/g, linoleic acid ranged from 30.13 to 39.31 mg FA/g, and the lowest was recorded for Arashidonic acid which ranged from 5.16 to 8.30 mg FA/g for the Gorgan and Sardasht populations, respectively.Industrial and practical recommendations: The oil and the essential fatty acids content of purslane leaves in the Sardasht population were higher than the Gorgan population. Since the oil and essential fatty acid content were affected by different climatic and cultivation factors, the western province of Iran is better suited for cultivation of medicinal plants having great oil and essential fatty acid contents. Manuscript profile
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  5 - Cytogenetic study of two Alhagi species
  مریم ابراهیم پورنورآبادی زهرا یزدان بخش مریم کشاورزی
  Background & Aim: Alhagi genus has two important medicinal plant species in Iran, including Alhagi pseudoalhagi, and A. graecorum (known “Kharshotor” in Persian). These plants are distributed in Iran like weeds. Generally, Alhagi is used as an anti-hemor More

  Background & Aim: Alhagi genus has two important medicinal plant species in Iran, including Alhagi pseudoalhagi, and A. graecorum (known “Kharshotor” in Persian). These plants are distributed in Iran like weeds. Generally, Alhagi is used as an anti-hemorrhoid, laxative and diuretic. Manna in both species produced by activity of a kind of insect on Alhagi branches which has medicinal properties and been used in Iranian traditional medicine. In this study, cytogenetic of 17 populations of A. graecorum and A. pseudoalhagi were studied. Experimental: In order to study the cytogenetic, pollen mother cells were studied in different meiotic developmental steps, and photographed. Pollen fertility test was done for studied populations. Data were statistically analyzed by SPSS software.Results & Discussion: Chromosome number in all species were 2n=2x= 16. Studied populations showed regular meiosis while some abnormalities as anaphase bridge in anaphase I, chromosomes stickiness in metaphase I, laggard chromosomes in metaphase I, and anaphase I, presence of rod and circular bivalent, univalent, quadrivalent and tri-polar were observed. Such structural modifications cause to increase in genetic variation and adaptation to environmental conditions. B chromosomes (I-V) were observed.Industrial and practical recommendations: Results of current study can lead to further researches in order to increase and optimize the medicinal effective substances in Alhagi by genetic modification. Manuscript profile
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  6 - Effect of jasmonic acid and salicylic acid on polyphenol and flavenoids in extract of Calendula officinalis L. flower
  عبدالله قاسمی پیربلوطی سید عباس موسوی هریس فرهنگ تیرگیر بهزاد حامدی
  Background & Aim: Marigold or Calendula officinalis L. is a annual herb belongs to the Asteraceae family. Marigold is a valuable medicinal plant which is used in pharmaceutical industry. In addition, this plant is cultivated as an ornamental plant. Aim of current st More

  Background & Aim: Marigold or Calendula officinalis L. is a annual herb belongs to the Asteraceae family. Marigold is a valuable medicinal plant which is used in pharmaceutical industry. In addition, this plant is cultivated as an ornamental plant. Aim of current study was to evaluate the effect of different concentrations of jasmonic acid (JA), and salicylic acid (SA) on phytochemical properties of Calendula officinalis L. flower. Experimental: A pot experiment was conducted in a completely randomized design with six treatments, and seven replications. The treatments, including four concentrations of SA (1, 10, 20, and 40 μmol/L), and JA in three levels (50, 100, and 200 μl/L). The treatments were sprayed before the early flowering stage of growth. Results & discussion: Results of analysis of variance indicated the various concentrations of the foliar application of JA, and SA do have significant impacts on polyphenol, carotenoid and flavenoids contents in the extracts of C. officinalis. In addition, results indicated the correlation between traits by Pearson method that there was a significant and positive relation between polyphenol and carotenoid (0.603**). Industrial and practical recommendations: In metabolic cultivation, for producing a special metabolite, the main agronomic and processing management should be regarded in order to increase the rate of essence and metabolites. Finally, the treatment of solution of JA 100 μl may be suitable because of increased content of carotenoid and polyphenol contents. Manuscript profile
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  7 - Effect of drought stress on some physiological properties and antioxidant activity of Achillea tenuifolia Lam.
  شیما غریبی بدرالدین ابراهیم سید طباطبایی قدرت الله سعیدی سید امیرحسین گلی مجید طالبی
  Background & Aim: Osmotic stress in plants lead to increase free radicals and plants use different mechanisms to scavenge these radicals. So, researchers apply stresses especially drought stress to increase secondary metabolites such as phenolics, and antioxidants. More

  Background & Aim: Osmotic stress in plants lead to increase free radicals and plants use different mechanisms to scavenge these radicals. So, researchers apply stresses especially drought stress to increase secondary metabolites such as phenolics, and antioxidants. One of the valuable medicinal plants is yarrow (Achillea tenuifolia) which is a perennial plant with different medicinal applications.Experimental: In order to assess the effect of drought stress, four different treatments (25%, 50%, 75% and 100% of Field Capacity) with four replicates was done in plastic pots at greenhouse. After 30 days, leaves of samples were used to evaluate total phenolics, antioxidant activity using three model systems including 1,1-diphenyl-2-picrylhydrazy (DPPH), ferric thiocyanate (FTC), and β-carotene-linoleic acid assay. In addition, malone dialdehyde (MDA), and proline content were evaluated.Results & Discussion: Drought stress in A.tenuifolia significantly increased total phenoloics, and antioxidant activity (in DPPH and β-carotene-linoleic acid assay). In addition, proline and malone dialdehyde were elevated by stress, while according to FTC model, there was no significant differences between 25% and 50% FC treatments.Industrial and practical recommendations: According to the results of present study, the 50% FC treatment can be introduced as acceptable drought level to produce sufficient phenolics and acceptable plant yield. Finally, more studies suggested for assessing the effect of drought stress on amount of other compounds in this medicinal plant. Manuscript profile
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  8 - Effect of ecological factors on chemical compounds of Verbascum songaricum leaves
  وحید کریمیان محمد رضا وهابی محمد فضیلتی مصطفی ترکش اصفهانی
  Background & Aim: Verbascum songaricum is a perennial herbaceous belongs to the Scrophulariaceae (snapdragon) family. Current study was done to study the environmental conditions effect on phytochemical properties of Verbascum songaricum leaves for extracting the ef More

  Background & Aim: Verbascum songaricum is a perennial herbaceous belongs to the Scrophulariaceae (snapdragon) family. Current study was done to study the environmental conditions effect on phytochemical properties of Verbascum songaricum leaves for extracting the effective materials.Experimental: This study was conducted at five range sites, including Hoze valley, Ghahiz, Someirom, Ghale Gadam, and Dena in two provinces (Isfahan and Kohghiluyeh va Boyer Ahmad) Iran. The specimens were collected in flower stage on July 2011. Leaves extract was obtained using digestion method and then analyzed by GM-MS to identify the main compounds in extract.Results & Discussion: Results indicated the ketone and alcoholic compounds in Ghazis and Someirom and Dena were the higher than Hoze Valley and Ghale Gadam regions. Soil pH was as an important ecological factor affect on the main components in the extract of V. songaricum leaves. The highest amount of hydro-carbonic and amine compounds detected in Ghazis and Someirom and Dena regions.Industrial and practical recommendations: According to the results, it is recommended that with respect to the recognition of some ecological factors of Verbascum songaricum, the fields for widespread cultivating and producing of this plant can be prepared. Manuscript profile