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    • List of Articles Abdollah Ghasemi Pirbalouti

      • Open Access Article

        1 - Phytochemical response of hyssop (Hyssopus officinalis L.) to foliar application of jasmonic acid
        عبدالله قاسمی پیربلوطی ابوالقاسم گرگیج مهدی رحیم ملک بهزاد حامدی
        Background & Aim:Jasmonic acid (JA) is regarded as endogenous regulators that play important roles in regulating stress responses, plant growth and development.Effect of foliar application of JA on the essential oil and oil chemical components of hyssop (Hyssopus of More
        Background & Aim:Jasmonic acid (JA) is regarded as endogenous regulators that play important roles in regulating stress responses, plant growth and development.Effect of foliar application of JA on the essential oil and oil chemical components of hyssop (Hyssopus officinalis L.) were investigated. Experimental: This study conducted in a CRD with three replications and in experimental greenhouse, I.A.U., Shahrekord Branch, Iran at 2012. Experimental treatments included (I) water foliar application (control), (II) water + aceton foliar application (as a solvent), (III-V) 50, 100 and 200 JA µL. The essential oils obtained by hydro-distillation were analyzed by Gas Chromatography-Mass Spectrometry. Results & Discussion: The results of GC-MS showed that the major components of the oil were cis-3-pinanone (10-39%), trans-3-pinanone (4-28%) and β-pinene (27-34%). The results of analysis of variance of the experiment showed that different levels of the foliar application of JA do have significant impacts on chemical constitutes in the essential oil.Finally, foliar application of 200 µL JA on some of secondary metabolite production in H. officinalis oil could be partially changed. Recommended applications/industries: Abbreviation: JA: Jasmonic Acid, MJ: Methyl Jasmonate, GC-MS: Gas Chromatography-Mass Spectrometry µL: Micro liter, M: Molar; ANOVA: Analysis of Variance, CRD: Completely Randomized Design. Manuscript profile
      • Open Access Article

        2 - Micropropagation of Clematis orientalis L. culture in vitro
        علی ایزدی صادق آبادی احمد خلیقی عبدالله قاسمی پیربلوطی مرضیه تقی پوردهکردی
        Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as anornamentalplant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this st More
        Background & Aim: Clematis is an ornamental and medicinal plant that is primarily planted as anornamentalplant. Clematis orientalis is native to Asia and Central Europe, used as an antiseptic and refrigerant. The propagation of Clematis is very important. In this study, the effects of plant growth regulators on rooting and growth of the plants in vitro culture were examined. Experimental: In this study, effects of growth regulators, including naphthalene acetic acid (NAA), at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL and Indol butyric acid (IBA) at the concentrations of 0.0, 0.4, 0.5, and 0.6 mg/mL on micropropagationin Murashige and Skoog’s medium (MS) were investigated. Results & Discussion: Results indicated that the effects growth regulators of auxin at different concentrations on in vitro growth micrpropagules were significant. The highest rooting and number of stems were obtained from control. Increase concentration of growth regulators decreased rooting. The highest decrease rate was obtained from0.6 mg/L auxin. Recommended applications/industries: The result of study indicated that positive effects lateral shoot explants and Murashige and Skoog’s medium (MS) without growth regulators on micropropagation. Manuscript profile
      • Open Access Article

        3 - Effect of foliar application of Jasmonic Acid (JA) on essential oil yield and its compositions of Thymus daenensis Celak
        محمد اشرافی عبدالله قاسمی پیربلوطی مهدی رحیم ملک بهزاد حامدی
        Background & aim: Recent researches have been done on effects of plant growth regulators (PGR) on growth and production of different crops whose results indicate the effectiveness of most of these hormones on qualitative and quantitative growth of herbs. The applica More
        Background & aim: Recent researches have been done on effects of plant growth regulators (PGR) on growth and production of different crops whose results indicate the effectiveness of most of these hormones on qualitative and quantitative growth of herbs. The application of regulators and inhibitors of growth on herbs may, in addition to growth, also lead to secondary metabolites stimulation. Jasmonic acid (JA) and its volatile methyl ester (MJ) collectively termed jasmonates, are regarded as endogenous regulators that play important roles in regulating stress responses, plant growth and development. This study was carried out to survey the effect of spraying of jasmonic acid (JA) on T. daenensis Celak in Shahrekord, Southwest Iran at 2010-2011. Experimental: A pot experiment was conducted in a CRD with six treatments and seven replications. The treatments including I: distilled water (control), II: distilled water and solution (acetone), III-VI: JA in various concentrations (50, 100, 200 and 400 µM). The treatments were applied before flowering stage. The aerial parts were characterized by Clevenger approach and Gas Chromatography–Mass Spectrometry. Results & discussion: Results of analysis of variance of the experiment showed that different levels of the foliar application of JA do have significant impacts on chemical constitutes in the essential oil of T. daenensis. Various concentrations of foliar application of JA caused decrease or increase some of secondary metabolites in essential oil. In comparison with other treatments, there is a significant difference between amount of thymol and carvacrol, as two main compounds in essential oil of T. daenensis, having 100 µM JA. The application of acetone solvent with distilled water also caused the increase in some of constitutes of the essential oil. Industrial and practical recommendations: In metabolic cultivation, for producing a special metabolite, the main agronomic and processing management should be regarded in order to increase the rate of essence and metabolites. Finally, some of secondary metabolite production in Thymus daenensis Celak could be partially changed by supplementation of different elicitors such as JA. Manuscript profile
      • Open Access Article

        4 - Histopathological evidences for effect beneficial of Satureja hortensis extract on hepatic lesion by cadmium–induced in Rat
        الهام مقتدایی خوراسگانی عبداله قاسمی پیربلوطی ش. ادیبی
        Background & Aim:Cadmium is an important industrial and environmental pollutant. Cadmium is one of the most toxic and carcinogenic heavy metals to organisms. This heavy metal mainly distributes to the liver and kidney in humans and animal and, causing acute hepatic More
        Background & Aim:Cadmium is an important industrial and environmental pollutant. Cadmium is one of the most toxic and carcinogenic heavy metals to organisms. This heavy metal mainly distributes to the liver and kidney in humans and animal and, causing acute hepatic injury. Experimental: The ethanol extract of Satureja hortensis L. (Lamiaceae family), was evaluated for its activity against cadmium–induced in male Wister rats (150 – 180 g). The ethanol extract of S. hortensis (100 and 200 mg/kg/day for six weeks) was examined on serum bicochemical and hepatic histopathological characteristic of rats subcutaneously received with cadmium chloride (CdCl2) at 3 mg/kg/day for six weeks. Results: The biochemical results indicated that aspartate transminase (AST) and alanine transaminase (ALT) significantly increased in serum by cadmium–induced. The liver histopathological results revealed that the ethanol extract of S. hortensis treatment at 200 mg/kg/day significantly reduced toxicity by cadmium–induced. The ethanol extract of S. hortensis prevents the cadmium–induced lesions in hepatic function. Recommended applications/industries: Known antioxidant, antimicrobial, antihepatotoxic, nephroprotective potentials of the extract of S. hortensis may be the mechanisms by which this plant protects animals against experimentally cadmium–induced. Manuscript profile
      • Open Access Article

        5 - The influence of reduced irrigation on herbage, essential oil yield and quality of Thymus vulgaris and Thymus daenensis
        سید محمد علوی سامانی عبدالله قاسمی پیربلوطی مهرداد عطایی کچویی بهزاد حامدی
        Background & Aim: Drought stress, as a main abiotic stress, has a significant influence on growth and metabolic activities of plant species. In this study, the influence of reduced irrigation on dry herbage, essential oil yield, and chemical components of two thyme More
        Background & Aim: Drought stress, as a main abiotic stress, has a significant influence on growth and metabolic activities of plant species. In this study, the influence of reduced irrigation on dry herbage, essential oil yield, and chemical components of two thyme species were investigated. Experimental: This study was done in an experimental greenhouse, I.A.U., Shahrekord Branch, Iran at 2012 in a CRD with three replications. Three irrigation levels were I1 (irrigated in field capacity or control), I2 (slightdroughtstressor irrigation in 75% fieldcapacity), and I3 (milddroughtstressor irrigation in 50% fieldcapacity). Two thyme species included Thymus daenensis and T. vulgaris. The essential oils obtained by hydro-distillation and were analyzed by Gas Chromatography-Mass Spectrometry. Results & Discussion: Results indicated that irrigation levels had a significant effect on many morphological and physiological characteristics, including dry matter, plant height, and leaf area index (LAI). In addition, levels of irrigation affected on oil yield and some major constituents in the essential oils. Carvacrol, γ-terpinene, and p-cymene contents in the essential oils were significantly increased under stressed conditions, whereas thymol amount was significantly reduced under stressed conditions. Recommended applications/industries: According to the results of this study, droughtstress reduces the essential oil yields and dry herbage in both species of thyme. Manuscript profile
      • Open Access Article

        6 - Effect of jasmonic acid and salicylic acid on polyphenol and flavenoids in extract of Calendula officinalis L. flower
        عبدالله قاسمی پیربلوطی سید عباس موسوی هریس فرهنگ تیرگیر بهزاد حامدی
        Background & Aim: Marigold or Calendula officinalis L. is a annual herb belongs to the Asteraceae family. Marigold is a valuable medicinal plant which is used in pharmaceutical industry. In addition, this plant is cultivated as an ornamental plant. Aim of current st More
        Background & Aim: Marigold or Calendula officinalis L. is a annual herb belongs to the Asteraceae family. Marigold is a valuable medicinal plant which is used in pharmaceutical industry. In addition, this plant is cultivated as an ornamental plant. Aim of current study was to evaluate the effect of different concentrations of jasmonic acid (JA), and salicylic acid (SA) on phytochemical properties of Calendula officinalis L. flower. Experimental: A pot experiment was conducted in a completely randomized design with six treatments, and seven replications. The treatments, including four concentrations of SA (1, 10, 20, and 40 μmol/L), and JA in three levels (50, 100, and 200 μl/L). The treatments were sprayed before the early flowering stage of growth. Results & discussion: Results of analysis of variance indicated the various concentrations of the foliar application of JA, and SA do have significant impacts on polyphenol, carotenoid and flavenoids contents in the extracts of C. officinalis. In addition, results indicated the correlation between traits by Pearson method that there was a significant and positive relation between polyphenol and carotenoid (0.603**). Industrial and practical recommendations: In metabolic cultivation, for producing a special metabolite, the main agronomic and processing management should be regarded in order to increase the rate of essence and metabolites. Finally, the treatment of solution of JA 100 μl may be suitable because of increased content of carotenoid and polyphenol contents. Manuscript profile
      • Open Access Article

        7 - Micro-morphology of fruit and pollen in Satureja khuzistanica Jamzad.
        الهام معلم عبدالله قاسمی پیربلوطی صدیقه یوسف نعنایی
        Background & Aim: Satureja khuzistanica Jamzad. is an aromatic and medicinal perennial plant belongs to family mint (Lamiaceae). It is also an Iranian exclusive endemic species, its essential oil components are widely used in medicinal, culinary, and perfume industr More
        Background & Aim: Satureja khuzistanica Jamzad. is an aromatic and medicinal perennial plant belongs to family mint (Lamiaceae). It is also an Iranian exclusive endemic species, its essential oil components are widely used in medicinal, culinary, and perfume industries. Experimental: Flowers were collected in 10 regions in Khuzistan and Lorestan provinces, Iran at early anthesis during 2009. Micro-morphology of pollen and nutlet was studied through SEM observation. Results & discussion: Insignificant pollens of S. khuzistanica were hexacolpate, radially symmetrical, oblate-spheroidal, and isopolar. Exine sculpturing is bireticulate and pitted. The nutlets showed undulate-reticulate pattern formed by hexagonal cells. Industrial and practical recommendations: Micro-morphology comparison of nutlet and pollen in S. khuzistanica with other genus of Lamiaceae imports for taxonomy this genus. The S. khuzistanica pollen is like other types and species of family Lamiaceae and all of these differences and similarities can help in more exact taxonomy of this species, along with other researches. Manuscript profile
      • Open Access Article

        8 - Phytochemical diversity on essential oils from Myrtus communis leaves of different populations collected from Fars and Kohkeloyeh va Boyerahmad provinces
        لیلا مهرآور غلامرضا باژیان عبدالله قاسمی پیربلوطی مجتبی جعفرنیا
        Background & Aim: Myrtle (Myrtus communis L.), is an important medicinal plant that is used for pharmacological uses and conservation of environments (for example preventing the soil erosion) regards to its specific condition habitat. Experimental: In this research, More
        Background & Aim: Myrtle (Myrtus communis L.), is an important medicinal plant that is used for pharmacological uses and conservation of environments (for example preventing the soil erosion) regards to its specific condition habitat. Experimental: In this research, phytochemical variations of the essential oils from different populations of myrtle collected from Fars (Saydan, Cheshme-Ali, and Simakan), and Kohkeloyeh va Boyerahmad (Dehdasht and Gachsaran) provinces using GC-MS were analyzed. Results & Discussion: Results of GC/MS analysis indicated that α–pinene, 1,8–cineole, linalool, linalyl acetate, α–terpineol, and linalool oxide were the main components in the essential oils from all populations. The essential oils from the leaves of myrtle were characterized by high levels of oxygenated monoterpenes, hydrocarbon monoterpenes, phenylpropanoids and sesquiterpenes. Monoterpenes were the main constituents of the essential oil of the leaves of the collected plants. Cluster analysis based on Euclidean distance square by UPGMA method classified the samples into three groups. In this classification, samples referred to the Gachsaran and Dehdasht populations (Kohkeloyeh va Boyerahmad province), and the Saydan population (Fars province) were at the same group. Recommended applications/industries: Results indicated the main source of variability in chemical composition and oil yield of the studied populations seemed to be due to differences in environmental conditions and chemotypes. Manuscript profile
      • Open Access Article

        9 - Effects of essential oils of Thymus deanensis and Satureja bachtiarica on plasma lipoproteins in rats feeding with a fatty diet
        مهرنوش نظری رامش منجمی عبدالله قاسمی پیربلوطی محسن جعفریان دهکردی مجید ریاحی دهکردی
        Background & Aim: Hyper lipidemy is one of the main factors in progressing cardiovascular diseases. Thymus deanensis celak and Satureja bachtiarica Bunge are medicinal herbs which are used in folklore medicine in Iran. In this research, hypolipidemic effects of esse More
        Background & Aim: Hyper lipidemy is one of the main factors in progressing cardiovascular diseases. Thymus deanensis celak and Satureja bachtiarica Bunge are medicinal herbs which are used in folklore medicine in Iran. In this research, hypolipidemic effects of essential oils of these herbs in rats fed with a fatty diet were investigated. Experimental: 40 male and femade adult rats (20 rats in each group) with 190 - 220 g weight were divided to five groups randomly (8 rats in each group). Experimental groups were 1) control, 2) control + fatty ration, 3) standard drug () + fatty ration, 4) T. deanensis (200 mg/kg) + fatty ration, and 5) S. bachtiarica (200 mg/kg) + fatty ration. At one week after starting special dietary ration, animal treated by the essential oils for three weeks. In final, serum and plasma were separated for measuring lipids and doing the other biochemical experiments. Results & Discussion: Results indicated that the essential oil of T. deanensis in rats fed with fatty dietary ration decreased level of low density lipoproteins (LDL) significantly (p ≤ 0.05). There was a significant difference in amount of triglycerides of blood serum in rats (p ≤ 0.05), The lowest and highest amounts of triglycerides obtained from T. deanensis and control + fatty ration groups, respectively. Recommended applications/industries: Results indicated the essential oil of T. deanensis had hypolipidemic effect in rats fed with a fatty diet. Manuscript profile
      • Open Access Article

        10 - Effect of harvest times on quantity (morphological) and quality characteristics of Thymus daenensis Celak. in Isfahan
        احمدرضا گل پرور عبدالله قاسمی پیربلوطی حسین زینلی امین هادی پناه
        Background & Aim: Deanaie thyme (Thymus daenensis Celak.), a member of the Lamiaceae family. Thymus in one of the most important medicinal and aromatic plants that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed countries. Recent More
        Background & Aim: Deanaie thyme (Thymus daenensis Celak.), a member of the Lamiaceae family. Thymus in one of the most important medicinal and aromatic plants that was used in pharmaceutical, food, cosmetics hygienic industries in most of developed countries. Recent studies have showed that thyme have strong antibacterial, antifungal, spasmolytic and antioxidant activities.Experimental: This experiment was done in a random block design with three replications conducted in field of Islamic Azad University, Khorasgan (Isfahan) 2010. Treatment phonological stages such as: before flowering stage; at beginning of flowering stage; 50% flowering stage; at full flowering stage and at fruit set stageResults & Discussion: The results obtained in our study, showed that effects phonological stages had very significant effect (p<0.01) on plant height, diameter, fresh and dry herb weight and thymol percentage. The highest plant height (31.83 cm) and highest plant diameter (38.50 cm) were at the fruit set stage. The highest fresh herb weight (10621 kg/ha) and the highest dry herb weight (4243 kg/ha) was at the fruit set stage. The lowest Fresh herb weight (5545 kg/ha) and the lowest dry herb weight (2035 kg/ha) was at the before flowering stage. The highest essential oil content (1.41%) was extracted at the 50% flowering stage. The highest thymol content (84.1%) was extracted at the before flowering stage.Industrial and Practical Recommendations: According to the findings of this research, the fruit set stage can be introduce as a the most appropriate time of harvesting for gaining the highest function of wet and dry Thymus daenensis, but for achieving to highest rate of thymol , the flowering stage can be recommended. Manuscript profile
      • Open Access Article

        11 - Antioxidant activity of essential oil of Heracleum lasiopetalum fruits on chemical properties of potato chips
        لیلا صداقت بروجنی محمد حجت الاسلامی جواد کرامت عبدالله قاسمی پیربلوطی
        Background & Aim: Chemicals antioxidants and lipid oxidation products are known as cancer-causing factors in food systems. In this study, the effect of essential oil from Heracleum lasiopetalum fruits on lipid oxidation and physicochemical properties of potato chips More
        Background & Aim: Chemicals antioxidants and lipid oxidation products are known as cancer-causing factors in food systems. In this study, the effect of essential oil from Heracleum lasiopetalum fruits on lipid oxidation and physicochemical properties of potato chips in comparison with commercial antioxidant has been investigated. Experimental: The essential oil of H. lasiopetalum extracted using a Clevenger-type apparatus, and analyzed by GC/MS. The essential oil was added to the frying oil of potato chips in concentration level 3000 ppm, and its antioxidant effect on produced chips was analyzed by peroxide value, TBA and acidity of the essential oils from potato chips. BHA and TBHQ were added as the commercial antioxidant in 200 ppm and antioxidant reduction evaluated in 45 days period. Results & Discussion: Results indicated the essential oil of H. lasiopetalum fruits could reduce oxidation of potato chips the same as synthetic antioxidants. TBA test indicated the essential oil of H. lasiopetalum is higher effective than BHA in reduction of the primary TBA reaction but TBHQ is the lowest. Recommended applications/industries: The essential oil of H. lasiopetalum had antioxidant effect which it can use as natural antioxidant in foods during storage. Manuscript profile