Effect of explants and growth regulators on direct organogenesis of Dracocephalum kotschyi Boiss. via tissue culture technique

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

محمود اطرشی ¹ , کوثر مرادی ²

1 - پژوهشکده بیوتکنولوژی کشاورزی منطقه مرکزی کشور (ABRII)، جاده فلاورجان (بلوار پژوهش)، اصـفهان، ایران؛
2 - پژوهشکده بیوتکنولوژی کشاورزی منطقه مرکزی کشور (ABRII)، جاده فلاورجان (بلوار پژوهش)، اصـفهان، ایران؛

Received: 2012-08-31 Accepted : 2012-11-10 Published : 2012-11-01

Keywords: plant growth regulators, Dracocephalum kotschyi, Explants, Organoganasis,

Abstract :

Background & Aim: Dracocephalum genus belongs to the Lamiaceae family which has eight species in Iran. Dracocephalum kotschyi Boiss is an important medicinal and aromatic plant that use in pharmaceutical industry. In addition, this species is used in Iranian traditional medicine. The aim of current study was to examine the various growth regulations and explants on direct organogenesis of Dracocephalum kotschyi using tissue culture technique. Experimental: Various concentrations of BAP, IBA and NAA belong to auxin and cytokinin, as two important plant growth regulators (PGR), with shoot tip explants; hypocotyl and cotyledon were investigated in plant micropropagation of Dracocephalum kotschyi. Results & Discussion: Results indicated that the best treatment and explants on regeneration Dracocephalum kotschyi was MS medium containing 5 mg/L BAP, and 0.2 mg/L NAA on shoot tip explants. Regenerated explants on MS medium containing 1 mg/L BAP and 0.5 mg/L IBA had elongation growth and the elongation growth medium, plantlets were rooted. Rooted plantlets were then transferred to the hardening. After consistency of the regeneration plant seedlings Dracocephalum kotschyi, about 95% of seedling transferred to greenhouse suitable growing. Industrial and practical recommendations: According to the results of this research, we can start to proliferate and produce the effective matter of medicinal plant, such as Dracocephalum kotschyi. The most important benefit of this method is to prevent from extinction and to protect the species out of the environment.

References:

اطرشی، م.، توکلی دینانی، ا.، درزی، م.ت.، هاشمی، ج.، روزبه، ش. و معصومی، ا. 1390. تاثیر امواج فراصوت بر کارون در کالوس حاصل از کشت بافت زیره سیاه (Bunium persicum Boiss.). فصل­نامه داروهای گیاهی، 2: 136-129.

نجف پورنوائی، م. و میرزا، م. 1386. بررسی مقایسه ای ترکیب­های شیمیائی اسانس نمونه زراعی و رویشگاهی گیاه Dracocephalum kotschy .  فصل­نامه علمی- پژوهشی تحقیقات گیاهان داروئی و معطر ایران، 23: 133-128.

 

Agostini, G. and Echeverrigaray, S. 2006. Micropropagation of Cunila incisa Benth., a potential source of 1,8-cineole. Instituto de Biotecnologia, Universidade de Caxias do Sul, CP 1352, CEP 95001-970, Caxias do Sul,RS, Brasil Rev. Bras. Pl. Med., Botucatu 8:186-189.

Ewa, S. and Halina, W. 2004. In vitro regeneration of Salvia nemorosa L. from shoot tips and leaf explants. In Vitro Cellular & Developmental Biology Plant., 40: 596-602.

Gohari, A., Saeidnia, S., Matsuo, K., Uchiyama, N., Yagura, T. and Michiho, K. 2003. Flavonoid constituents of Dracocephalum kotschy growing in Iran and their trypanocidal activity. Natural Medicines., 57(6):250-252.

Golshani, S., Karamkhani, F., Monsef Esfehani, H.R. and Abdollahi, M. 2004. Antinociceptive effects of the essential oil of Dracocephalum kotschy in the mouse writhing test. Journal of Pharmaceutical Sciences., 7(1):76-9.

Irina, T., and Constantin, T. G. 2004. Histo-anatomy and in vitro morphogenesis in Hyssopus officinalis L. (Lamiaceae). Acta Botany Croat., 63:59–68.

Jahanian, F., Ebrahimi, S.A., Rahbar-Roshandel N. and Mahmoudian, M. 2005. Xanthomicrol is the main cytotoxic component of Dracocephalum kotschy and a potential anti-cancer agent. Phytochemistry., 66 (13):1581-92.

 Luciana, B. and Dode. 2003. In vitro propagation of Ocimum basilicum L. (Lamiaceae). Acta Scientiarum Biological Sciences., 2: 435-437.

Murashige, T. and Skoog, F. 1962. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant physiology., 15: 473–497.

Naima, B., and Taoufik, K. 2007. An efficient in vitro plant regeneration system for the medicinal plant Teucrium stocksianum Boiss Plant Biotechnology Reports., 1: 179-184.

Sebastiana, M. 2004. In vitro Callus Induction and Plants from Stem and Petiole Explants of Salvia canariensis L. Plant Tissue Culture., 14:167-172.

Saeidnia, S., Gohari, A., Ito, M. and Kiuchi, F. 2005. Bioactive constituents from Dracocephalum kotschy. Zeitschrift fur Naturforschung, C-Journal of Biosciences 60(1-2): 22-24.