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Open Access Article
1 - Synthesis of titanium-silver bimetallic nanoparticles by thermal plasma method
Sahar Sheini Shahrooz Saviz Amir Hossein Sari -
Open Access Article
2 - Silver nanoparticles dispersed in nematic liquid crystal: an impact on dielectric and electro-optical parameters
Rohit Katiyar Kaushlendra Agrahari Govind Pathak Tripti Vimal Geeta Yadav Kamal Kumar Pandey Abhishek Kumar Misra Atul Srivastava Rajiv Manohar -
Open Access Article
3 - Evaluation of anti-bacterial property of modified Nano silver packaging with Photocatalyst TiO2 in sheep’s head and trotters
Sharifan, A., Beheshti Zadeh, R., Ahari, H.*, Anvar, A. .Head and trotters sheep is traditional food in Iran. This product has very microbial load then for first time determine to done it. The current study was designed to evaluate application of silver nanoparticles, as an antimicrobial agent. Effect of Nano silver pack MoreHead and trotters sheep is traditional food in Iran. This product has very microbial load then for first time determine to done it. The current study was designed to evaluate application of silver nanoparticles, as an antimicrobial agent. Effect of Nano silver packaging in reduce microbial load in head and trotters compare with Control sample in the Refrigerator for 4°C for 1,2,3,4 days. Minced Head and trotters sheep (100g of product has 15 g fat, 90 ml⁄gr Cholesterol). this product has bought at Shahrvand mall. Nano - silver coating based on titanium dioxide was used to reduce the microbial load of the product. some Nano-coating of silver on titanium dioxide at a concentration of ppm 1000, 2000, 3000, 3500 and 4000 used at this study. For measurement nanoparticles size used Field Emission Scanning Electron Microscope (fesem) and to know Chemical bond used. The results showed The concentrations of silver nanoparticles ppm 4000 ppm 3500 on Escherichia coli maintaining the relative amounts of byproducts in four days of testing on impact (p<0.05). Average of nanoparticle was. Manuscript profile -
Open Access Article
4 - The effect of nanosilver oral administration on body function, some blood and biochemical parameters of serum in chickens
M. Rahim, M. Talebi mehrdar,Silver has long been known for its antibacterial properties. In fact, silver nanoparticles, due to the release of silver ions, show such properties against aerobic and anaerobic bacteria and are widely used as additives in poultry diets. Regarding th MoreSilver has long been known for its antibacterial properties. In fact, silver nanoparticles, due to the release of silver ions, show such properties against aerobic and anaerobic bacteria and are widely used as additives in poultry diets. Regarding the antibacterial effects of this compound, research has shown contradictory results that do not indicate beneficial antibacterial effects. For this study, 240 one-day-old male chickens were divided into six groups with four replications. The experimental groups were: control diet (without additives), diet containing 10 ppm of the antibiotic avilamycin, diet containing 400 ml of silver nanoparticles per ton of feed, diet containing 800 ml of silver nanoparticles per ton of feed, diet containing 400 ml of silver nanoparticles per cubic meter of drinking water, diet containing 800 ml of silver nanoparticles per cubic meter of drinking water and to investigate the effect of silver nanoparticles on growth factor and body efficiency and some blood and serum parameters and microbial population of the device Digestive (ileum) of broiler chickens and comparison of antibacterial and growth stimulant effects with avilamycin antibiotic.The results showed a significant increase (P <0.05) in monocytes and chicken feed consumption and an increase in Clostridium population and a significant decrease in triglyceride concentration in the 800 ml group of silver particles in the diet and water (P <0.05), but its effect There was no significant effect on chicken weight gain and feed conversion ratio. Contrary to popular belief, silver nanoparticles are not suitable as a growth stimulant additive to control harmful bacteria in the poultry digestive tract and strengthen the immune system, increasing the feed conversion ratio of broilers. Manuscript profile -
Open Access Article
5 - The Study of Antimicrobial Effect of Silver Nanoparticles Biosynthesized by the Leaf Aqueous Extract of Kelussia odoratissima Mozaff. against Some Pathogenic Microbes with Food Sources
O. Azizian Shermeh M. Taherizadeh M. Valizadeh A. Ghasemi M. Beigomi A. Kamali DeljooIntroduction: Growth of food pathogenic microorganism is one the major obstacles in the foodindustry. Nano technology has a broad application in all parts of food industry. The developmentof bacterial resistance to antibiotics and antimicrobial effect of silver nanopart MoreIntroduction: Growth of food pathogenic microorganism is one the major obstacles in the foodindustry. Nano technology has a broad application in all parts of food industry. The developmentof bacterial resistance to antibiotics and antimicrobial effect of silver nanoparticles, has caused theattention of researches to this subject. In this study, biosynthesis of silver nanoparticles and theeffective parameters concerned and the antimicrobial effect of them against some food pathogensfor use in antimicrobial packaging has been discussed.Materials and Methods: Silver nanoparticles were synthesized by the leaf aqueous extract ofKelussia odoratissima Mozaff. In order to achieve Silver nanoparticles with a uniform shape andminimum size, effective parameters on synthesis such as: pH of reaction, extract volume,concentration of Ag+ and reaction time, were studied and optimized by UV-Visspectrophotometry. After characterization of nanoparticles using TEM and XRD techniques, theirantimicrobial activities were investigated against Staphylococcus aureus, Bacillus cereus,Escherichia coli, Salmonella tifimurium,Listeria monocytogenes 4b, Aspergilus Flavus,Penicillium expansum, Clavicpes purpurea by Agar Well Diffusion and MIC, methods.Results: Silver nanoparticles that had been biosynthesized with the approximate size of 20-25nm,had high antimicrobial activity against all of microorganisms and this activity was dependent onthe concentration of silver nanoparticles, therefore in very low concentrations, they prevented thegrowth of microorganisms.Conclusion: Biosynthesis of silver nanoparticles, made nanoparticles with minimum size andbetter performance. Currently these nanoparticles can be use in food industry as food disinfectantfilters, food coatings and packaging and cleaning the food pruduction lines. Manuscript profile -
Open Access Article
6 - Evaluation of the Effect of Polyethylene Coating Based on Silver Nanoparticles on Increasing the Shelf Life, Qualitative and Microbial Parameters of Wheat Germ
M. Darab Sh. Shahriari H. MozafariIntroduction: It is possible to increase the shelf life and maintain the quality of wheat germ during storage by using suitable packaging. One of the new technologies considered by researchers is the use of packaging based on nanoparticles.Materials and Methods: In this MoreIntroduction: It is possible to increase the shelf life and maintain the quality of wheat germ during storage by using suitable packaging. One of the new technologies considered by researchers is the use of packaging based on nanoparticles.Materials and Methods: In this study, wheat germs were packaged in polyethylene packages containing silver nanoparticles, and the effect of the packaging on the quality characteristics and shelf life of wheat germ were determined. In this research work, at determining the most optimal conditions for the packaging of wheat germ, the effects of weight percent (wt%) of nanosilver (0, 3, and 5%), time of storage (0, 5, and 10 days), and three different temperatures (2, 4, and 6 0C) on the shelf life of wheat germ have been studied.Results: The results showed that temperature, storage time, and concentration of nanosilver in polyethylene packaging had significant effects on moisture, pH, and enzyme activity of wheat germ. Experimental data indicated that the storage time of wheat germ increased by increasing the concentration of nanosilver from 3% to 5% and by decreasing temperature from 6 ° C to 2 ° C for ten days. Nanoparticle packages were significantly (P <0.05) able to reduce mold growth in wheat germ.Conclusion: Based on the results, it might be concluded that the application of films based on silver nanoparticles increases the shelf life of wheat germ. Wheat germ had the highest shelf life in packaging with 5% silver nanoparticles at 2 ° C and was selected as the selected sample. Manuscript profile -
Open Access Article
7 - Synthesis and Characterization of Silver Nanocomposite as a Food Packaging
M. Pooyamanesh H. Ahari A. A. Anvar G. Karim -
Open Access Article
8 - Synthesis of Silver Nanoparticles with Sodium Brohydrate by Chemical Revival Method to Produce Biodegradable Antibacterial Silver Nanocomposite by Solution Blending Production of biodegradable antibacterial silver nanocomposites
Zahra Ta'ati jafroudi hamed ahari nekisa sohrabi haghdostBackground and Objective: This study aimed to investigate the antibacterial effect of silver nanoparticles on Gram-negative and Gram-positive bacteria and producing silver nanocomposites with suitable and biodegradable antibacterial properties by Solution Blending metho MoreBackground and Objective: This study aimed to investigate the antibacterial effect of silver nanoparticles on Gram-negative and Gram-positive bacteria and producing silver nanocomposites with suitable and biodegradable antibacterial properties by Solution Blending method. Method: To investigate the antimicrobial properties of silver nanoparticles, silver nanoparticles were first synthesized by NaBH4 reduciton and after performing UV-VIS, DLS, XRD and TEM and FT-IR tests, Staphylococcus aureus as a Gram-positive, Escherichia coli as a Gram-negative bacteri was used, investigated by MIC, MBC and Inhibition Zone. In the next step, the nanocomposite was prepared and prepared by Solution Blending method and its antibacterial activity was evaluated by Inhibition Zone method. Findings: The results showed that silver nanoparticles had inhibitory effect on Staphylococcus aureus, Escherichia coli and Candida albicans at concentrations of 50, 20 and 355 μg / ml respectively and also at concentrations of MIC. 60, 40 and 370, respectively, have the lethal effect on the aforementioned microorganisms (MFC, MBC). A diameter of 3-5 mm was observed around the silver nanoparticles in the cultures of the mentioned microorganisms and in the test of nanocomposite Inhibiion zone, the halo was created based on expected values.the results of the experiments were calculated with 3 replications and the amount of PValue was significant (P<0/0001)by ANOVA method.we used also 3 antibiotic as control for Inhibition zone test. Discussion and Conclusion: Silver nanoparticles showed good inhibitory and lethal effects on Gram-positive and Gram-negative bacteria and Also, the nanocomposites produced by the mentioned method have high antibacterial and viscolastic properties. Manuscript profile -
Open Access Article
9 - Investigating the morphophysiological characteristics and shelf life of Alstromeria spp. with the application of salicylic acid after harvesting
مهناز قنبری الهام دانائیThis research was designed in order to increase the quality and longevity of Alstroemeria cut flowers after harvesting. In this study, the puls treatment of salicylic acid at three levels of 50, 100 and 150 mg/liter and then the preservative solution of silver nanoparti MoreThis research was designed in order to increase the quality and longevity of Alstroemeria cut flowers after harvesting. In this study, the puls treatment of salicylic acid at three levels of 50, 100 and 150 mg/liter and then the preservative solution of silver nanoparticles 2 mg/liter with 3% sucrose was used. Distilled water and silver nanoparticles with sucrose were used as controls. The experiment was conducted as a factorial in the form of a completely randomized statistical design with 5 treatments and 3 replications (each treatment included 5 Alstroemeria cut flower branches). Sampling and measurement of desired traits were done on the 5th, 10th and 15th days of the experiment. The results showed that the highest relative fresh weight, solution absorption rate, percentage of cell membrane stability index and shelf life of Alstroemeria cut flowers were in salicylic acid 150+ silver nano particles+ sucrose Treatment. The highest amount of soluble solids, anthocyanin content and total chlorophyll was obtained in salicylic acid 100+ silver nanoparticles+ sucrose treatment. Therefore, concentrations of 100 and 150 mg/L of salicylic acid with nanoparticles 2 mg/L and sucrose 3% can be recommended to improve the morphophysiological characteristics and shelf life of Alstromeria spp. Manuscript profile -
Open Access Article
10 - Effects of Silver Nanoparticles on Urinary Tract Infections Caused by Gram-negative Bacilli Resistant to Several Antibiotics (MDR)
نشاط غلامی amir hossein MOMEN مسعود زندیAbstractOne of the major problems in hospitals resistance of pathogenic bacteria , including bacteria that cause urinary tract infections(UTI), antimicrobial substances , the problem of increased costs of treatment , treatment failure and mortality rates are increasing. MoreAbstractOne of the major problems in hospitals resistance of pathogenic bacteria , including bacteria that cause urinary tract infections(UTI), antimicrobial substances , the problem of increased costs of treatment , treatment failure and mortality rates are increasing.The purpose of this research is to identify multidrug-resistant Gram-negative bacilli of urinary tract infections with antibiotics and the effect of silver nanoparticles on them.Planting 240 clinical samples from patients admitted to two hospitals ( Fatemiyeh , Besat) within 6 months of 1395 a total of 150 gram negative bacilli of urinary tract infections was isolated. To determine antibiotic resistance of these bacteria, disk diffusion method was used, and their susceptibility was evaluated according to the CLSI(clinical and laboratory Standards Institute) . Gram-negative bacilli isolated were prepared and inhibition zone diameter was measured.The most common bacteria Escherichia coli was isolated and lowest frequencies of the Proteus vulgaris. All of the samples to a solution of silver nanoparticles at concentrations 200 ppm 100 ppm and were sensitive. Pseudomonas aeruginosa (30 mm) and Enterobacter aerogenes (27 mm) maximum diameter in the presence of silver nanoparticles showed 800 ppm.Conclusion: The results show that silver nanoparticles can have an inhibitory effect on all tested Gram-negative bacilli , and the concentration of nanoparticles , the diameter of the growth of Gram-negative bacilli of urinary tract infections resistant to multiple antibiotics increases.Key words: Gram-negative bacilli, Silver nanoparticles, Urinary Tract Infection,Antibiotic Resistance, Hamedan Manuscript profile -
Open Access Article
11 - study the effect of silver nanoparticles against to biofilm in listeria monocytogenes
razieh ahmadinasab soheil Aghaei Mohammad Ali GasemzadehListeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, MoreListeria monocytogenes has been isolated from many foods and may cause meningitis, septicemia and abortion in pregnant women. L. monocytogenes forms biofilm on many food contact surface materials and medical devices. Biofilm formation by this bacterium on many surfaces, is one of the most important problems in the food industry. Metal nanoparticles are effective antimicrobial compounds for controlling and removing of bacterial biofilms from non-biomaterial surfaces. The main purpose of this investigation was synthesis of silver iodide nanoparticles in-vitro condition and evaluation of their antimicrobial properties for inhibition of biofilm formation and biofilm eradication of Listeria monocytogenes(ATCC1298). Method: In this study nano-particles of silver iodide were synthesized respectively sonochemically and were confirmed using UV-Vis ,FTIR, EDX and Scanning Electron Microscopic procedures. Antimicrobial and antibiofilm properties of synthesized nanoparticles were determined using agar well diffusion and 96 well microplate dilution methods respectively. Results: Synthesized Silver nanoparticles had circular structure with 30 nm size. Antibiofilm activity and biofilm eradication of Iodide silver nanoparticles were obtained at 5 and 100 µg/ml respectively. Discussion: Based on obtained results, silver iodide nanoparticles can be used as the effective antimicrobial agents for inhibiting of biofilm formation of Listeria monocytogenes in the food industry. Manuscript profile -
Open Access Article
12 - Biosynthesis of Silver Nanoparticles by a native Halotolerant Actinomycete strain Isolated from Soils Qom, Iran
sheida nadernia soheil Aghaei Mohammad Ali GasemzadehAbstract Background:In recent years, ability of microorganisms to synthesize nanoparticles of various sizes, shapes and morphologies, has gained extreme attentions. the point of this study is Biosynthesis of Silver Nanoparticles by a native Halotolerant Actinomycete str MoreAbstract Background:In recent years, ability of microorganisms to synthesize nanoparticles of various sizes, shapes and morphologies, has gained extreme attentions. the point of this study is Biosynthesis of Silver Nanoparticles by a native Halotolerant Actinomycete strain Isolated from Soils of Saline Lake Qom Materials & methods: In the present study, synthesis and characterization, of silver nanoparticles from native isolate of halotolerant Actinomycete strain has been reported. Silver nanoparticles were synthesized by adding the dried biomass of halotolerant actinomycete isolate with 10-3M of nitrate solution adjusted pH 7.2 and in M9 liquid medium. Silver nanoparticles were characterized using ultraviolet and visible absorption spectroscopy, infrared spectroscopy (UV-VIS) Fourier Transform Infrared Spectroscopy(FT-IR), beam X Ray Diffraction(XRD), Dynamic light scattering (DLS), and imaging with Transmission Electron Microscope (TEM). Results:The results obtained from ultraviolet and visible absorption spectroscopy, infrared spectroscopy (UV-VIS) Fourier Transform Infrared Spectroscopy(FT-IR) and beam X Ray Diffraction (XRD), Of the halotolerant actinomycetes strains, the isolate NO.3 was able produce silver nanoparticles under different environmental conditions. Dynamic light scattering (DLS), and imaging with Transmission Electron Microscope (TEM) showed that the average size of silver nanoparticles obtained 28 to100 nm. The results indicated that production of silver nanoparticles was extracellular. Conclusion:The results showed that the native halotolerant actinomycete strains can synthesize silver nanoparticles under different environmental conditions . Keywords: silver nanoparticles, Biosynthesis, halotolerant actinomycetes. Manuscript profile -
Open Access Article
13 - The effect of silver nanoparticles on the expression of exotoxin A and S genes in Pseudomonas aeruginosa
Muhammad Hemati Zahra Keshtmand Katayoun BorhaniBackground: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been wide MoreBackground: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been widely considered in the field of medicine and medicine, especially against microbes. The aim of the present study was to investigate the effect of silver nanoparticles (AgNPs) on the expression of Pseudomonas aeruginosa exotoxin A and S genes. Methods: In this experimental study, the minimum growth inhibition concentration of silver nanoparticles was determined by microdilution method. First, the lowest concentration of bacterial growth inhibitor is determined with silver nanoparticles. Then, at a concentration lower than the lowest growth rate, the expression of Pseudomonas aeruginosa exotoxin A and S genes was examined by Real time PCR. Data were analyzed using one-way ANOVA, Tukey test and P value less than 0.05. Findings: Silver nanoparticles with a concentration of 62.5 μg / ml had growth inhibitory effects on Pseudomonas aeruginosa. Also, at a concentration of 31.25, the expression of Pseudomonas aeruginosa exotoxin A and S genes was significantly reduced (p < 0.05). Conclusion: The results of this study show that lower concentrations (31.25 μg/ml) than growth inhibition concentrations (62.5μg/ml) can reduce the expression of Pseudomonas aeruginosa exotoxin A and S genes. Manuscript profile -
Open Access Article
14 - Green bio-synthesis of Silver Nanoparticles Using Ziziphora tenuior L Water Extract
Sajjad Sedaghat Parvin Afshar -
Open Access Article
15 - Antifungal Activity of Silver Nanoparticle in Different Sizes against Some Pathogenic Fungi
Monir Aghamoosa Azar Sabokbar -
Open Access Article
16 - Understanding the phytochemical constitution, antioxidant potential and spectral characteristics of aqueous extracts of the chosen leafy vegetables from south India
Sobha Kota Pradeep Dumpala Hariseetharam Prasad Dachepalli Anantha Ratna Kumari -
Open Access Article
17 - Effect Of Lead Chloride on Embryonic Stages and Kidney Differentiation in Pterophyllum Scalare
Sara Shekari mehrengiz sadooghi Homayon HosseinzadehIntroduction and Objective: The amount of heavy metals in the environment has increased with human industrial interference and cause damage to the environment provides.Free of heavy metals are found in combination with other elements in the environment , and mineral wat MoreIntroduction and Objective: The amount of heavy metals in the environment has increased with human industrial interference and cause damage to the environment provides.Free of heavy metals are found in combination with other elements in the environment , and mineral waters. Effect of lead chloride on fish embryos that named (Pterophyllum scalar) is the aim of this studyTherefore, 5 pairs of ornamental fish of a species to determine the toxicity of lead chloride in the process of embryonic development (early life) before the eggs were extractedMaterials and Methods: In the first study to determine the acute toxicity LC50 concentration of the desired material (lead chloride) 5 concentration of 3 μg/L, 5 μg/L, 10 μg/L and 20 μg/L and control was performed in three successive iterations .Each test was repeated for 3 days and counting the number of dead embryos (white) Acute toxicity of lead chloride at doses less than μg/L (5 μg/L ≤ n) was determinedIn the second phase of research to examine the changes desired effect of causing toxicity on fetal developmental stages of Angel fish were. Stages of the test is less than the LC50 concentration of less than 4 were considered g/Lμ5. The concentrations of 5/0, 5/1, 3 and 5/4 g / Lμ and controls for comparisonSome of these samples at the same moment sampling survey was study with photo stereo microscope.Results:The results showed that lead chloride in the initial review abnormally apparent like the tilt, loss of vision or the lack of effect on growth delay. Histological studies of tissues prepared for light microscopy study also showed that lead chloride on growth of embryonic kidney (Prenefroses) did not interfere Manuscript profile -
Open Access Article
18 - Effect Ofsilver nanoparticle injection on body weight, liver index and spleen index in rat
Hossein Hamzehei Hamed Alizadeh Nader Hajizadeh Mohammad Taghi MoazedInroduction and Objective:Silver nanoparticles may be toxic. The mechanism of toxicity is not known. Great concern for the health of humans in nature has created. The changes in body weight, liver index and spleen index can be effective in identifying harmful factor in MoreInroduction and Objective:Silver nanoparticles may be toxic. The mechanism of toxicity is not known. Great concern for the health of humans in nature has created. The changes in body weight, liver index and spleen index can be effective in identifying harmful factor in the body. This study was performed to examine the effects of silver nanoparticles on body weight and liver and spleen index in rats.Materials and Methods:21 adult female rats were randomly divided into 7 groups. The mice were weighed. Group 1 was sham. Control group 2 and group 3 to 7 experimental groups were treated respectively with 5, 10, 20, 40 and 80 ppm of silver nanoparticles. Mice treated for 10 days. On the eleventh day the rats were killed by diethyl ether anesthesia. The mice were weighed and then dissected; the liver and spleen were removed. Liver and spleen were weighed in order to calculate their index.Results:The results showed no significant change in body weight of mice, spleen weight, liver weight and liver index and spleen index.Conclusion:Intraperitoneal injection of silver nanoparticles does not affect the index in rat liver and spleen. Manuscript profile -
Open Access Article
19 - Effect Of Injection Of Silver Nanoparticles on Plasma Levels Of Liver Enzymes in Rat
Hossein Hamzehei Hamed Alizadeh Mohsen Ajalli Farid DolatshahiBackground and Purpose:Possible toxic effects of silver nanoparticles on humans are a major concern that encountered the use of this nanoparticles with many challenges. Today, silver nanoparticles, mostly because of their antimicrobial effects have been discussed. But t MoreBackground and Purpose:Possible toxic effects of silver nanoparticles on humans are a major concern that encountered the use of this nanoparticles with many challenges. Today, silver nanoparticles, mostly because of their antimicrobial effects have been discussed. But the possibility of their side effects, make its use doubtful. Among the main effects of silver nanoparticles that can have in the body, effects on the liver and its metabolism which has not been mostly considered. The aim of this study was to examine the injected effects of silver nanoparticles on plasma levels of liver enzymes such as AST, ALT and ALP that could open new horizons in relation to current challenges.Methods:In this study, 21 female Wistar rats were used. The rats were randomly divided into 7 groups. The first group was sham group, the second group was control group, the third to seventh groups were tested, were treated with 5, 10, 20, 40 and 80 ppm of silver nanoparticles, respectively. Treatment of mice continued for 10 days. Then the rats were killed with ether anesthesia. Blood samples were taken from the hearts of animals. Plasma levels of liver enzymes (AST, ALT and ALP) were tested by diagnostic kit of Pars Azmoon company using indirect spectrophotometric method.Results:The results showed that there are no significant differences between the control and sham groups. But overall, silver nanoparticles at high concentrations cause significant changes in levels of enzymes.Conclusions:The results of this study showed that silver nanoparticles at high concentrations can have deleterious effects on the liver and should be used with caution in high concentrations Manuscript profile -
Open Access Article
20 - The Antibiotic Resistance Pattern of Methicillin Resistant Staphylococcus aureus and Antibacterial Effect of Silver and Copper Nanoparticles on that In vitro and Animal model
Saba Hashemi Rasoul ShokriBackground & aim: Considering the high resistance to drug in methicillin-resistant Staphylococcus aureus, this study was performed to investigate the effects of silver and copper nanoparticles and their combined effect on the methicillin-resistant Staphylococcus aur MoreBackground & aim: Considering the high resistance to drug in methicillin-resistant Staphylococcus aureus, this study was performed to investigate the effects of silver and copper nanoparticles and their combined effect on the methicillin-resistant Staphylococcus aureus in vitro and animal models.Materials & Methods: To determine the most effective antibiotics, disk diffusion method was performed. The microdilution method is used for determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of copper and silver nanoparticles and their combination . Then, to examine the effect of nanoparticles and the antibiotic, experiments were performed in the animal models. For this purpose, after intraperitoneal inoculation of bacterial suspension, MBC concertrations obtained from microdilution method were injected to the mice and after the cultivation of spleen, colony count was performed.Results: The most effective antibiotic on bacteria was vancomycin. The MIC of silver and copper nanoparticles, vancomycin, silver and copper nanoparticles, silver nanoparticles-vancomycin and copper nanoparticles- vancomycin were 1250 ppm, 2500 ppm, 78/12, 625 ppm, 35 ppm and 250 ppm respectively. In animal model, the lowest number of colonies is related to silver nanoparticles and then silver nanoparticles-vancomycin.Conclusion: The results show that antimicrobial effect of silver nanoparticles is more than vancomycin and copper nanoparticles. Also the combination of silver and copper nanoparticles increases their antimicrobial strength and the combination of silver and vancomycin after complementary experiments, can be a option for the treatment of infections caused by these bacteria. Manuscript profile -
Open Access Article
21 - Deletion of Auramine O and Crystal Violet from Industrial Aqueous Solution on to Albizia Lebbeck Leaves-Capped Silver Nanoparticles Synthesis Investigating Parameters Equilibrium
Fatemah Maghami Maryam Abrishamkar -
Open Access Article
22 - Synthesis Silver Nanoparticles by Recovery Silver from Anode Slime of Kerman Sarcheshmeh Cooper Complex
H. Zahedi A. Mollahosseini E. Noroozian -
Open Access Article
23 - Synergistic Effects of Taxus baccata Extract Mixtures with Silver Nanoparticles against Bacteria and Fungal
M. Ghaedi M. Yousefi Nejad L. Delshad -
Open Access Article
24 - The effect of green silver nanoparticles on spinach (Spinacia oleracea) seed germination under salt stress
Amin Ali Naghi Zadeh Mehrnaz Mahmoudi Zarandi Sayed Mohammad Reza Khoshroo Fatemeh Hasan ZadehSalinity is one of the most important abiotic stresses for plants and its adverse effects on different stages of plant growth and yield have been reported. Salinity can reduce seed germination as the most sensitive stage of plant growth. The use of silver nanoparticles MoreSalinity is one of the most important abiotic stresses for plants and its adverse effects on different stages of plant growth and yield have been reported. Salinity can reduce seed germination as the most sensitive stage of plant growth. The use of silver nanoparticles has been suggested to improve the germination of some plants in salinity conditions. On the other hand, consuming spinach is highly recommended due to its high nutritional value. In this study, aloe vera extract was used for synthesis of silver nanoparticles. Then, the effects of salinity on seed germination of spinach and the role of silver nanoparticles on seed germination indices in this plant were investigated. The formation of silver nitrate nanoparticles was investigated using a spectrophotometer, XRD analysis, and electron microscopy. Spherical nanoparticles, with an average size of 11.97 nm to 29.46 nm were observed. Treatments in a completely randomized design with three replications of green silver nanoparticles at concentrations of 10, 20, 40, 80 and 100 ppm and salinity at four levels of 0, 50, 100 and 150 mM on spinach seed germination process were investigated . The results showed that the germination process under salinity conditions decreased significantly compared to the control group. Treatment of 40 ppm concentration of silver nanoparticles for different levels of salinity had a significantly increased effect on germination indices at 1% level . Manuscript profile -
Open Access Article
25 - The effect of synthetic silver nanoparticle from hydroalcoholic extract Digitalis nervosa on CDH1, Bax , Bcl2 and P53 gene expressions in cervical cancer cell line)Hela(
nazila ahmadzadeh nastran asghari moghaddam zahra keshtmandNowadays, metal nanoparticles, especially silver, due to their special physical properties and anti-cancer effects have been considered.One of the methods of synthesis of these nanoparticles is the use of plant extracts. The aim of this study was to investigate the anti MoreNowadays, metal nanoparticles, especially silver, due to their special physical properties and anti-cancer effects have been considered.One of the methods of synthesis of these nanoparticles is the use of plant extracts. The aim of this study was to investigate the anti-cancer effects of silver nanoparticles synthesized of the hydroalcoholic extract of Digitalis nervosa on the cancer cell line (Hela).In this experimental study,the synthesis of silver nanoparticles was performed by adding the hydroalcoholic extract of Digitalis nervosa to the silver nitrate solution. Physical and chemical properties of synthesized nanoparticles were determined by UV-vis, TEM, SEM, EDX and XRD methods.Viability of Hela cancer cells by MTT method with different concentrations of synthesized silver nanoparticles(3.125 to 100 mg/ml) and the expression of Bax, Bcl2, P53 and CDH1 genes were evaluated using real time PCR technique in 24 hours.Data were analyzed using GraphPad software and one-way ANOVA test.P<0.05 was considered statistically significant.The results showed that the results of cytotoxicity at different concentrations of silver nanoparticles showed that IC50 was16.66 μg /ml, respectively. Also, the expression of Bax, CDH1 and P53 apoptotic genes in concentration-(16.66μg/ml) increased and Bcl2 gene expression decreased compared to the reference gene, which showed the effects of cytotoxicity and changes in nanoparticle concentration. Silver nanoparticles synthesized from the extract of the Digitalis nervosa plant have a cytotoxic effect and possibly induce apoptosis on Hela cancer cells. Manuscript profile -
Open Access Article
26 - Study of Antibacterial Synergistic Effect of Origanum vulgare L. Essential Oil and Silver Nanoparticles
Nahid mohammadzadeh Maasuomeh Mahdavi-Ourtakand Sahar Honarmand JahromiThe increasing use of antibiotics and the prevalence of resistant strains have necessitated the use of new antimicrobial drugs. Combination therapy is an effective strategy to combat antibacterial resistance. The aim of this study was to investigate the antibacterial sy MoreThe increasing use of antibiotics and the prevalence of resistant strains have necessitated the use of new antimicrobial drugs. Combination therapy is an effective strategy to combat antibacterial resistance. The aim of this study was to investigate the antibacterial synergistic effect of Origanum vulgare essential oil and silver nanoparticles on gram-positive and gram-negative bacteria. In this study, O. vulgare essential oil was extracted from the plant branch. A colloidal solution of silver nanoparticles with an average particle diameter of 68 nm was purchased. The antibacterial effect of O. vulgare essential oil and silver nanoparticles was investigated alone and then in combination by broth microdilution method and their minimum inhibitory concentration (MIC) against gram-positive and gram-negative bacteria was determined. The bacteria studied were Escherichia coli, Salmonella enterica, Bacillus subtilis and Staphylococcus aureus. Based on the obtained results, the synergistic effect of O. vulgare essential oil and silver nanoparticles had significant antimicrobial effects on all studied bacteria. The results showed that the synergistic effect of O. vulgare essential oil and silver nanoparticles was effective in inhibiting and killing the studied bacteria. And they can be useful in developing new drugs against these bacteria. Manuscript profile -
Open Access Article
27 - Effect of silver nanoparticles on surface hydrophobicity and biofilm formation in Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli
alireza Farazandeh Fatemeh Noorbakhsh Sahar Honarmand JahromiIntroduction: Biofilms are microbial aggregates that adhere to a substrate as a surface and are surrounded by an exopolysaccharide matrix. The bacteria that make up biofilms are so resistant to antimicrobials and antibiotics that this has led to concerns in the medical MoreIntroduction: Biofilms are microbial aggregates that adhere to a substrate as a surface and are surrounded by an exopolysaccharide matrix. The bacteria that make up biofilms are so resistant to antimicrobials and antibiotics that this has led to concerns in the medical community.The aim of this study was effect of silver nanoparticles on surface hydrophobicity and biofilm formation in Staphylococcus aureus, Acinetobacter baumannii, Pseudomonas aeruginosa and Escherichia coli. Methods: This study was performed on 40 bacterial strains including Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Acinetobacter baumannii isolated from Milad Hospital in Tehran. The strains were identified by biochemical tests. Also, biofilm formation by microtiter plate method, surface hydrophobicity by MATH method and sensitivity of strains to silver nanoparticles were evaluated.Results: According to the results obtained in this study, all strains were able to form biofilms and no strains with negative biofilms were observed in the study. The highest effect of silver nanoparticles in this study was observed on strains at concentrations of 0.5, 1 and 2 μg / ml. Also, only 20% of Pseudomonas aeruginosa strains in this study were observed with moderate hydrophobicity and the others of the bacteria were weakly hydrophobic.Conclusion: silver nanoparticles in low concentrations with the effect of low toxicity had the ability to inhibit the biofilm formation of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Acinetobacter baumannii. Hydrophobicity in the studied bacteria were weak. Manuscript profile -
Open Access Article
28 - Green production of silver nanoparticles from )Eryngium planum( and its antimicrobial effect on )Escherichia coli( and )Staphylococcus aureus(
zahra khoshhal Atoosa Vaziri rahele rahbarianBackground: The development of nanotechnology in the last decade has opened a new and unique window to science; Among them, silver nanoparticles are one of the most important types of nanoparticles due to their antimicrobial properties. There are different methods for p MoreBackground: The development of nanotechnology in the last decade has opened a new and unique window to science; Among them, silver nanoparticles are one of the most important types of nanoparticles due to their antimicrobial properties. There are different methods for producing silver nanoparticles by green synthesis. Using plant extracts to produce silver nanoparticles is a cheap and cost-effective method that does not harm the environment.Objective: In this research, the phytochemistry of the plant and the production of silver nanoparticles using the leaves of )Eryngium pulegium( were studied. Also, the antibacterial effect of the resulting nanoparticles was investigated on Escherichia coli and Staphylococcus aureus bacteria.Methods: First, secondary metabolites including phenol, flavonoids, terpenoid and alkaloids were measured. Then, the amount of antioxidant activity of Chuchag) Eryngium pulegium) plant extract was measured by DPPH method and after confirming the presence of antioxidant property and high amount of secondary metabolites in the examined plant, the synthesis of silver nanoparticle was carried out by aqueous extract of Chuchag plant)Eryngium pulegium( and by spectrophotometry and SEM methods. TEM confirmed the presence of nanoparticles and determined the size and shape of nanoparticles. Then, the antibacterial property of the extract along with nanoparticles was evaluated on two species of bacteria, Escherichia coli and Staphylococcus aureus.Findings: The analysis of secondary metabolites in (Eryngium pulegium) plant showed that the main and indicative compounds in (Eryngium pulegium) essential oil include terpenoids curcumin, gamma-3 carne, beta gorgonen, alpha guain, gamma cadinene, alpha terpinen, etc. The results of LC.MS of alkaloids showed that Eryngium pulegium has 4 alkaloid compounds in order of highest concentration, berbamine, coxoline, balochistin and fikamin. The results related to DPPH in (Eryngium pulegium) plant showed that as the concentration of ( Eryngium pulegium) extract increased, its free radical inhibition percentage also increased, so it can be proved that Eryngium pulegium has free radical inhibitory properties. It also has antioxidant properties. Vis-UV spectroscopic analysis and the presence of a peak at 370 nm indicated the biosynthesis of these nanoparticles in (Eryngium pulegium) extract, and the SEM image determined the shape of the nanoparticles to be spherical and their average size around 20 nm. In the investigation of the antibacterial effect of nanoparticles biosynthesized with (Eryngium pulegium) extract against microbial bacteria, the nanoparticles showed a good antibacterial effect against the studied bacteria. In the MIC test on Staphylococcus aureus bacteria, the minimum inhibitory concentration in(Eryngium Manuscript profile -
Open Access Article
29 - A review of the antimicrobial and antibacterial effects of silver nanoparticles and silver composites
Zahra Javanshir Maryam MohammadpoorToday, nanoparticles include a wide range of particles with unique properties. Due to their small size and unique properties, these particles can be used in various sciences. Nanoscale biological compounds have unique physical-chemical properties that are In recent year MoreToday, nanoparticles include a wide range of particles with unique properties. Due to their small size and unique properties, these particles can be used in various sciences. Nanoscale biological compounds have unique physical-chemical properties that are In recent years, recently, the efficacy of antimicrobial nanoparticles, especially silver nanoparticles and their antimicrobial activity, has been the focus of researchers and researches have been conducted. In this study, recent advances and research results of researchers in the use of antibacterial and antiseptic properties of silver nanoparticles, silver and silver composites have been reviewed.The search was done on SID and Google Scholar sites. We did not have a time limit for the search, and all the articles that met the entry criteria were included in the study until March 2018. Among the articles found, some of the articles with the conditions including the complete article have antibacterial and anti-infective properties. 10 articles were evaluated. All the articles have anti-bacterial and anti-infective properties of Silver nanoparticles, silver and silver composites have been in different levels. Manuscript profile -
Open Access Article
30 - The effect of prebiotic pretreatment on histological lesions of Nile tilapia fry (Oreochromis niloticus) exposed to silver nanoparticles
farahnaz kakavand Aliakbar Hedayati maryam rezaei shadegan ali jafar nodeh saeed maddahThe presence of emerging nanoparticle contaminants in aquatic environments reduces the safety performance of aquatic animals, so the use of safety stimuli is essential. The aim of the present study was to investigate the effect of different prebiotic levels of oyster mu MoreThe presence of emerging nanoparticle contaminants in aquatic environments reduces the safety performance of aquatic animals, so the use of safety stimuli is essential. The aim of the present study was to investigate the effect of different prebiotic levels of oyster mushrooms (Pleurotus ostreatus) on tissue damage in the liver and gills of tilapia exposed to silver nanoparticles. For this purpose, 120 juveniles of Tilapia (Oreochromis niloticus) were divided into 4 experimental groups with 3 replicates and bred for 42 days including treatment 1 as control (fed with diet without probiotic) and treatments 2-4, fed with diet containing 0.05%, 0.1% and 0.2% prebiotics, then 5 ppm of silver nanoparticles were added to the culture medium of each treatment for 16 days. At the end of the experimental period, fish were anesthetized with clove anesthetic solution (220 mg/L) and their liver and gill tissues were isolated for histological studies. Treatments exposed to silver nanoparticles showed extensive complications in gill tissue as well as severe injuries and bile stasis in liver tissue. However, the use of mushroom prebiotics was able to reduce the destructive effects of silver nanoparticles on these tissues. Overall, the results indicated that treatment with 0.2% of prebiotics in the diet could have the best protective effect on tissue damage in the liver and gills of tilapia exposed to silver nanoparticles. Manuscript profile -
Open Access Article
31 - Effects of silver nanoparticles synthesized through chemical reduction on plasma superoxide dismutase and glutathione peroxidase enzymes in rat model
elham ghavidelaghdam mohamad narimanirad alireza lotfiAbstract With possibility of synthesis of silver nanoparticles in citrate-coated form and via chemical method, the aim of this study was synthesis of silver nanoparticles by chemical reduction method and investigation of the impact of nanoparticles on super MoreAbstract With possibility of synthesis of silver nanoparticles in citrate-coated form and via chemical method, the aim of this study was synthesis of silver nanoparticles by chemical reduction method and investigation of the impact of nanoparticles on superoxide dismutase and glutathione peroxidase enzymes in an animal model. Silver nanoparticles were synthesized by chemical reduction with a mixture of distilled water and sodium borohydrate and adding silver nitrate and sodium citrate to the obtained solution. In vivo study was conducted using 40 adult male rats with an average weight of 100 grams. Animals were divided into four groups, as control, placebo, and treatment groups receiving silver nanoparticle solutions (100 and 200 mg/kg, respectively). The treatment groups received silver nanoparticle solutions (100 and 200 mg/kg) on the first and the seventh days of experiment intraperitoneally. Oxidative effects of injected high concentrations of silver nanoparticles (200 mg/kg) lead to mortality in the experimental animals. Infusion of silver nanoparticles at concentrations of 100 and 200 milligrams per kilogram of body weight decreased the activity of plasma superoxide dismutase (SOD) and glutathione peroxidase (GPx). This reduction was significantly higher (p<0.01) at the dose of 200 mg/kg. In conclusion, studies on animal models showed that the concentrations of 100 and 200 milligrams per kilogram of body weight of silver nanoparticles synthesized by chemical reduction method decreases the activity of antioxidant enzymes, eventually leading to mortality. Manuscript profile -
Open Access Article
32 - Antibacterial effects of green laser in vicinity of silver nanoparticles on methicillin-resistant staphylococcus aureus (MRSA)
Maliheh Ranjbaran Zahra Aghaebrahimi Jamshid Sabaghzadeh zohreh daraeizadeh arash abednezhad -
Open Access Article
33 - Determine optimal conditions of synthesis of silver green nanoparticles by aqueous extract of Falcaria vulgaris edible- medicinal plant and evaluation of its antioxidant properties
Ghazaleh Kouchakzadeh Zeinab DelfanNowadays, the advent of nanotechnology and the synthesis of nanoparticles have brought many developments to humans, but this technology must be considered in terms of safety. Meanwhile, the synthesis of green nanoparticles has expanded due to more compatibility with the MoreNowadays, the advent of nanotechnology and the synthesis of nanoparticles have brought many developments to humans, but this technology must be considered in terms of safety. Meanwhile, the synthesis of green nanoparticles has expanded due to more compatibility with the environment and that they are low costs and non-toxic, because the plants have a very good ability for reducing the metal ions and synthesizing nanoparticles. In recent research, the optimum conditions for the green synthesis of silver nanoparticles using the extract of Falcaria vulgaris Bernh. and its antioxidant effect were investigated. In this research, first aerial parts of plant were collected plains south of Khorramabad before the flowering stage in May and dried in shaid. After preparing the aqueous extract of plant by soaking, effective parameters of nanoparticles synthesis such as pH, AgNO3 concentration, extract concentration, temperature and time were evaluated. Optimal conditions of silver nanoparticles synthesis by plant are include pH=11, AgNO3 concentration= 8 mM, extract value= 4 cc, temperature= 40̊C and time= 120 minute. The formation of silver nanoparticles was confirmed by the techniques of UV-Vis, X ray diffraction and FT-IR. The study of the morphology of the resulting nanoparticles showed a spherical shape with a particle size of 16-29 nm. Evaluation of antioxidant activity of aqueous extract and synthesizing nanoparticle by conventional DPPH method were carried out. Maximum value of free radical inhibition at 12.5 μl/ml concentration of extract and nanoparticle compared to ascorbic acid was reported. Finally, it can be concluded that the presence of various chemical compounds that have caused the antioxidant activity of the extract expresses the extract and the resulting nanoparticles as a source of natural antioxidants. Manuscript profile -
Open Access Article
34 - Biogenic Synthesis of Silver Nanoparticles Using Fruit Aqueous Extract of Psidium Guajava and Its Antibacterial Activity
Babak Sadeghi Bita Koupaei -
Open Access Article
35 - Investigate The Effect Of Extracts On The Green Synthesis Of Silver Nanoparticles
Nooshin Amini Gholamreza Amin Zahra Jafari azar -
Open Access Article
36 - Preparation and Characterization of Curcumin-Silver Nanoparticle and Evaluation of the Effect of Poly Ethylene Glycol and Temperature
Parisa Adibzadeh Negar Motakef-Kazemi -
Open Access Article
37 - Aloe Vera gel extract and sunlight mediated synthesis of silver nanoparticles with highly effective antibacterial and anticancer activity
Noha Mohamed Hossam Elmasry -
Open Access Article
38 - Green synthesis and antibacterial application of silver nanoparticles using Oak Peel extract
Nooshin Japalaghi Nahid Ghasemi Majid Ramezani Behrooz Shojaei saadiThe use of various plant materials for the synthesis of nanoparticles is considered as green synthesis, which does not involve any harmful chemicals. In this study, silver nanoparticles were synthesized by a fast, one-step method and a green biosynthesis method by regen MoreThe use of various plant materials for the synthesis of nanoparticles is considered as green synthesis, which does not involve any harmful chemicals. In this study, silver nanoparticles were synthesized by a fast, one-step method and a green biosynthesis method by regenerating a silver nitrate solution using Oak peel extract containing biomolecule which as a reducing and stabilizing element Suitable to play a role. Effective parameters such as pH of solution, silver nitrate concentration, temperature and contact time were studied and optimized The structure and properties of nanoparticles were determined by spectroscopic absorption analyzes UV-Visible, Scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. According to the Sherer-Debye equation, the size of the nanoparticles was measured 29 nm. Then the antibacterial effect of nanoparticles produced against two strains of pathogenic bacteria, gram positive Staphylococcuas aureus and gram negative Escherichia coli was investigated by disk diffusion and determination of minimum inhibitory concentration(MIC) methods. The results of the antibacterial activities test showed that the nanoparticles produced from Oak Peel had a good effect on both bacteria. The purpose of this research is to synthesis and develop a new method for the preparation of silver nanoparticles using environmental methods. Manuscript profile -
Open Access Article
39 - Green synthesis of silver nanoparticles and antioxidant activities in Bunium persicum (Boiss.) B.Fedtsch. seeds extract .
Nastaran Kazemivash Ahmad Majd Sayyeh Jafari Marandi Seyed Mahdi Rezayat -
Open Access Article
40 - Histopathological study on acute toxicity of nanochelating based silver nanoparticles in mouse model
Seyedeh Mahsan Hoseinialfatemi Abdollah Karimi Fatemeh Fallah Shahnaz Armin Maryam Hafizi Somaye Kalanaki -
Open Access Article
41 - Biosynthesis of silver nanoparticles by Achillea eriophora DC. extract
Gholamreza Zarei Navid Baladi -
Open Access Article
42 - Aloe Vera gel extract and sunlight mediated synthesis of silver nanoparticles with highly effective antibacterial and anticancer activity
Noha Mohamed H.M. El-Masry -
Open Access Article
43 - Preparation and Characterization of Curcumin-Silver Nanoparticle and Evaluation of the Effect of Poly Ethylene Glycol and Temperature
Parisa Adibzadeh Negar Motakef-Kazemi -
Open Access Article
44 - Histopathological study on acute toxicity of nanochelating based silver nanoparticles in mouse model
Seyedeh Mahsan Hoseini-Alfatemi Fatemeh Fallah Abdollah Karimi Maryam Hafizi Shahnaz Armin Somaye Kalanaki -
Open Access Article
45 - Biosynthesis of silver nanoparticles by Achillea eriophora DC extract
Navid Baladi Gholamreza Zarei -
Open Access Article
46 - Green synthesis of silver nanoparticles and antioxidant activities in Bunium persicum (Boiss.) B.Fedtsch. seeds extract
Nastaran Kazemivash Ahmad Majd Sayeh Jafari Marandi Seyed Mahdi Rezayat -
Open Access Article
47 - Investigation of the Effect of Extracts on the Green Synthesis of Silver Nanoparticles
Nooshin Amini Gholamreza Amin Zahra Jafari Azar -
Open Access Article
48 - Antimicrobial Effect of Biosynthesized Silver Nanoparticles Using Rosemary (Rosmarinus officinalis) Extract on Some Soft Rot Bacteria in Storage Condition
Esmaiil Torkabadi Zeinab Fotoohiyan fatemeh shahdadiNowadays, use of plants in the synthesis of metal nanoparticles, which have antimicrobial activity, is considered. The aim of present study was evaluating biosynthesis of silver nanoparticles from aqueous extract of rosemary and then investigating the effect of these na MoreNowadays, use of plants in the synthesis of metal nanoparticles, which have antimicrobial activity, is considered. The aim of present study was evaluating biosynthesis of silver nanoparticles from aqueous extract of rosemary and then investigating the effect of these nanoparticles and mancoseb toxin on soft rot bacteria isolated from carrots, potatoes and onions during storage. The biosynthesized silver nanoparticles were analyzed by ultraviolet and FTIR spectroscopy. Soft rot bacteria were isolated and identified using selective and specific culture media from carrots, potatoes and onions. A pathogenic isolate from each sample with the highest pathogenicity was selected for the tests and their minimum inhibitory concentration (MIC) was evaluated against the experimental treatments. The MIC of the pathogen isolates against the 2580 mg/ml of silver nanoparticles and 1000 ppm of mancoseb were 88.88% and 100%, respectively. The mean percentage of vegetable contamination with pathogen isolate in the presence of silver nanoparticles in the third month of storage was 70%, 80% and 70% for onions, potatoes and carrots, respectively, and average percentage of vegetable contamination with pathogenic isolates in the combined control of bacteria with silver nanoparticles and mancoseb toxin at the third month of storage for onion, potato and carrot were 45, 40 and 40%, respectively. According to obtained results, the combined control of biosynthesized silver nanoparticles and Mancoseb can be used to control of soft rot bacteria in carrot, potato and onion. Manuscript profile -
Open Access Article
49 - Rapid Synthesis of Silver Nanoparticles and Their Decoration on TiO2 by Plasma-Over-Liquid Process: Characterization and Application for Tetracycline Antibiotic Degradation
Margarita Skiba Victoria Vorobyova -
Open Access Article
50 - پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی
ا. عباسی س.ر. هاشمی س. حسنی م. ابراهیمیهدف از این آزمایش ارزیابی پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی میباشد. به همین منظور در این آزمایش از 375 قطعه جوجه گوشتی یکروزه نژاد کاب (500 Cobb) استفاده شد. که در 5 تیمار Moreهدف از این آزمایش ارزیابی پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی میباشد. به همین منظور در این آزمایش از 375 قطعه جوجه گوشتی یکروزه نژاد کاب (500 Cobb) استفاده شد. که در 5 تیمار و 5 تکرار و 15 قطعه جوجه در هر تکرار در قالب یک طرح کاملاً تصادفی تقسیم شدند. پنج تیمار غذایی شامل: 1) جیره شاهد، 2) جیره شاهد حاوی 1 درصد زئولیت، 3) جیره شاهد حاوی 1 درصد زئولیت پوشش داده شده با 0.5 درصد نانو نقره، 4) جیره شاهد حاوی 1 گرم بر کیلوگرم اسید آلی و 5) جیره شاهد حاوی 1 درصد زئولیت پوشش داده شده با 0.5 درصد نانو نقره و 1 گرم بر کیلوگرم اسید آلی بود. خوراک مصرفی و وزن بدن برای هر تیمار در کل دوره آزمایش ثبت و ضریب تبدیل خوراک محاسبه گردید. به منظور شمارش جمعیت میکروبی دستگاه گوارش دو قطعه جوجه از هر واحد آزمایشی در روزهای 21 و 42 پرورش کشتار شدند. نتایج آزمایش نشان داد که جیره غذایی حاوی نانو نقره به همراه اسید آلی باعث کاهش وزن بدن جوجههای گوشتی در مقایسه با تیمار شاهد و زئولیت در فاصله سن 42-21 روزگی شد (P<0.05). همچنین در کل دوره پرورش ضریب تبدیل غذایی در گروه نانو نقره پوشش داده شده بر زئولیت به طور معنی ­داری نسبت به گروه شاهد و زئولیت بالاتر بود (P<0.05). با این حال تیمارهای آزمایشی هیچگونه اختلاف معنیداری بر جمعیت میکروبی دستگاه گوارش نداشتند (0.05<P). به طور خلاصه نتایج حاضر نشان داد اگرچه نانو ذرات نقره و اسیدهای آلی اثر خاصی بر پارامترهای عملکرد و افزایش شمار باکتریهای مفید (اسید لاکتیک) دستگاه گوارش در حالت تنش گرمایی نداشتند اما موجب کاهش آنها نگشته­اند. بنابراین میتوان از آنها به عنوان افزودنی در جیره غذایی جوجههای گوشتی استفاده نمود. Manuscript profile -
Open Access Article
51 - Effect of biologically synthesized silver nanoparticles on Melissa officinalis L.Evaluation of growth parameters, secondary metabolites and antioxidant enzymes
Asiyeh Shavalibor Sedigheh Esmaeilzadeh Bahabadi -
Open Access Article
52 - Evaluation of the effect of plant growth substances on longevity of gerbera cut flowers cv. Sorbet.
Elham Danaee Vahid Abdossi -
Open Access Article
53 - Effects of Silver Nanoparticle Exposure on Growth, Physiological and biochemical Parameters of Dracocephalum moldavica L.
Zahra Haghighi Pak Naser Karimi Hossein Abbaspour -
Open Access Article
54 - اثر تیمار کوتاه مدت با نانو ذرات نقره و محلول گلجایی ساکارز روی پیری گل و برگ شاخه بریده رز
خدیجه آل کثیر رحیم نقش بند حسنی علیرضا مطلبی آذراین آزمایش برای ارزیابی اثر تیمار کوتاه مدت نانو ذرات نقره در غلظت­ های صفر، 4، 25، 75 و 125 میلی­ گرم در لیتر روی گل شاخه بریدنی رز رقم ʼهای اند مجیکʻ بدنبال نگهداری در محلول ساکارز با غلظت­ های صفر، 2 و 3 درصد از نظر عمر گلجایی و برخی از صفات فیزیولوژیکی پ Moreاین آزمایش برای ارزیابی اثر تیمار کوتاه مدت نانو ذرات نقره در غلظت­ های صفر، 4، 25، 75 و 125 میلی­ گرم در لیتر روی گل شاخه بریدنی رز رقم ʼهای اند مجیکʻ بدنبال نگهداری در محلول ساکارز با غلظت­ های صفر، 2 و 3 درصد از نظر عمر گلجایی و برخی از صفات فیزیولوژیکی پس از برداشت آن انجام شد. این تحقیق به شکل آزمایش فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار برای هر تیمار اجرا شد. آزمایش تحت شرایط دمای 2 ± 23 درجه سانتی­گراد، رطوبت نسبی 5±60 درصد و با شدت نوری محیط 12 میکرو مول در مترمربع درثانیه به مدت 12 ساعت توسط لامپ­ های فلورسنت انجام شد. نتایج نشان داد که تیمار 125 میلی­ گرم در لیتر نانو ذرات نقره همرا با ساکارز دو درصد و بدون ساکارز بیشترین تاثیر را در کنترل باکتری­ های انتهای ساقه را داشت. تیمار کوتاه مدت نانو ذرات نقره در کلیه غلظت­ ها سبب بهبود جذب محلول، حفظ رطوبت نسبی شاخه، بهبود باز شدن گل و شاخص فلورسانس کلروفیل برگ (Fv/Fm) در مقایسه با تیمار شاهد گردید. بیشترین میزان باز شدگی گل با درشت­ترین اندازه گل در تیمار 75 میلی­ گرم در لیتر نانوذرات همراه با ساکارز 2 درصد در روز نهم عمر گلجایی مشاهده گردید. کمترین میزان عمر گلجایی در تیمارهای آب مقطر به تنهایی و همراه با ساکارز بود. بیشترین میزان عمر گلجایی در تیمارهای 75 و 125 میلی­ گرم در لیتر نانو ذرات نقره بدون ساکارز و با ساکارز در مقایسه با سایر تیمارها مشاهده گردید. Manuscript profile -
Open Access Article
55 - بهبود عمر پس از برداشت گل های شاخه بریده آلسترومریا ’Konst Coco‘ با استفاده از ترکیب روش های مکانیکی و شیمیایی
مهدی حکیمی اصل احمدرضا بریموندی به ­منظور بررسی اثر متقابل روش ­های مکانیکی (شکاف ته ساقه) و شیمیایی (نانوسیلور) بر عمر گلجایی و خصوصیات بیوشیمیایی گل­ های شاخه بریده آلسترومریا ’Konst Coco‘ آزمایشی فاکتوریل بر پایه طرح کاملا تصادفی با 10 تیمار، 3 تکرار، 30 پلات و 150 شا More به ­منظور بررسی اثر متقابل روش ­های مکانیکی (شکاف ته ساقه) و شیمیایی (نانوسیلور) بر عمر گلجایی و خصوصیات بیوشیمیایی گل­ های شاخه بریده آلسترومریا ’Konst Coco‘ آزمایشی فاکتوریل بر پایه طرح کاملا تصادفی با 10 تیمار، 3 تکرار، 30 پلات و 150 شاخه گل انجام شد. تیمارهای آزمایشی شامل شکاف طولی انتهای ساقه در دو سطح (بدون شکاف و شکاف 5 سانتی­ متری) و نانوسیلور در 5 سطح (0، 5، 10، 20 و 30 میلی­ گرم در لیتر) بودند. نتایج نشان داد که اثر متقابل تیمارهای آزمایشی روی همه صفات ارزیابی شده از نظر آماری معنی­ دار است. طبق نتایج مقایسه میانگین­ ها، بیشترین عمر گلجایی (13/88 روز) با کاربرد "شکاف 5 سانتی­ متری× 20 میلی­ گرم در لیتر نانوسیلور" حاصل شد که نسبت به شاهد 6/83 روز ماندگاری این گل شاخه بریده را افزایش داد. بیشترین جذب آب (2/03 میلی­ لیتر در هر گرم وزن تر)، ماده خشک (14/11 درصد)، کلروفیل کل (2/432 میلی­ گرم در هر گرم وزن تر) و کاروتنوئید گلبرگ (2/307 میکروگرم در هر گرم وزن تر) و کمترین کاهش وزن تر (1/34 گرم)، باکتری انتهای ساقه و محلول گلجایی (3 کلنی)، اتیلن (0/807 نانولیتر در لیتر در ساعت در هر گرم وزن تر)، نشت یونی (6/04 درصد)، MDA (12/53 نانومول در هر گرم وزن تر در دقیقه)، فعالیت آنزیم­ های SOD (12/64 واحد آنزیم در هر گرم وزن تر در دقیقه) و POD (0/09 نانومول در هر گرم وزن تر در دقیقه) برای تیمار "شکاف 5 سانتی­ متری× 20 میلی­ گرم در لیتر نانوسیلور" ثبت شد که موفق­ ترین تیمار در حفظ عمر گلجایی و خصوصیات بیوشیمیایی گل­ های شاخه بریده آلسترومریا‘Konst Coco’ بود. Manuscript profile -
Open Access Article
56 - Evaluation of the protective effect of Nigella sativa oil on ovarian follicles in mice treated with silver nanoparticles
SMA shariatzadeh Mozhgan Khagavi JafarabadAim: the effect of Nigella sativa oil (NSO) as an efficient antioxidant on ovarian follicles following treatment with Silver Nanoparticles (SNP) in adult mice. Material and methods: Twenty-four adult NMRI mice, weighing 27-30g were used in the present experiment. The an MoreAim: the effect of Nigella sativa oil (NSO) as an efficient antioxidant on ovarian follicles following treatment with Silver Nanoparticles (SNP) in adult mice. Material and methods: Twenty-four adult NMRI mice, weighing 27-30g were used in the present experiment. The animals were kept under optimum conditions of temperature (At 21± 2 ° C and ambient lighting conditions 12 hours of darkness and 12 hours of light) humidity and maintained on standard pellet diet with adequate water. They were divided into four groups of (six mice per group): control; Silver Nanoparticles (300 mg/kg/day, orally), Nigella sativa oil (5 ml/kg/day, orally) and finally Silver Nanoparticles plus Nigella sativa oil. After 30 days, the mice were sacrificed and the ovary tissues were separated. The results were analyzed using one-way ANOVA and Tukey’s test, and the means were significantly different at P<0.05. Results: The results of this study showed that, the mean total volume of ovary and the number of primordial, primary, secondary and graph follicles significantly decreased in the Silver Nanoparticles group compared with the control group (p<0.05). Nigella sativa oil significantly increased the above parameters in the SNP +NSO group compared to silver nanoparticles group (p<0.05). Furthermore, the number of follicles and total volume of ovary significantly increased in the Nigella sativa oil treated mice compared to the control ones (p<0.05) (Table 1). Conclusion: So it is not far-fetched that this oil has improved the factors in our study. Manuscript profile -
Open Access Article
57 - Efficient removal of copper ions from aqueous medium using a green synthesis of silver nanoparticles mediated by Mentha spicata leaf extract combined with electrospun polyamide 6 nanofibers: Isotherm and kinetics adsorption study
Shima Mahdavi Ghajari Mohammad Reza Allah Gholi Ghasri Sajjad Sedaghat Forogh Adhami Sima HabibiIn this study, the efficient and fast adsorption of copper ions on silver/polyamide 6 (Ag/PA 6) nanofibers from an aqueous solution were investigated. Green synthesis of silver nanoparticles (AgNPs) was obtained from the leaf extract of the Mentha spicata. The character MoreIn this study, the efficient and fast adsorption of copper ions on silver/polyamide 6 (Ag/PA 6) nanofibers from an aqueous solution were investigated. Green synthesis of silver nanoparticles (AgNPs) was obtained from the leaf extract of the Mentha spicata. The characterization of synthesized AgNPs was investigated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). Also, polyamide 6 and silver-polyamide 6 nanofibers were analyzed with a scanning electron microscope. To optimize the experimental factors, one factor at a time (OFAT) was used. The maximum removal percentage was obtained at pH equal to 5, contact time of 75 min, the adsorbent dosage of 2 g, and initial concentration of 20 mg/L. The isotherm models showed that the Langmuir isotherm with the coefficient of determination (R2) equal to 0.9976 was chosen as the best fitting model to describe the adsorption process. The maximum adsorption capacity (qmax) was obtained at 71.42 mg/g. In addition, the pseudo-second-order kinetic model showed a better fit for the removal of copper ions (R2=0.9994). The results showed that Ag/PA 6 can be used as a simple, efficient and environmentally friendly adsorbent to remove pollutants such as heavy metals from aqueous environments. Manuscript profile -
Open Access Article
58 - Preparation of Double-layer Nanofibers Base on Hydrogel, Silver nanoparticles, and Fatty Acid using electrospinning technique
ensieh ghasemian lemraski elaheh khajehali sahar yariToday, electrospinning method is widely used to produce nanofibers based on polymers. Poly (vinyl-alcohol) and chitosan are among the most widely used polymers in biochemistry and medicine, especially in wound healing and tissue engineering. In this project, an attempt MoreToday, electrospinning method is widely used to produce nanofibers based on polymers. Poly (vinyl-alcohol) and chitosan are among the most widely used polymers in biochemistry and medicine, especially in wound healing and tissue engineering. In this project, an attempt has been made to make polymeric materials and silver nanoparticles, nanofibers with protection and antimicrobial capabilities. Electrospinning method for making bilayer nanofibers, top layer containing poly (vinyl alcohol) and chitosan containing silver nanoparticles or silver ions as antimicrobial layer, and bottom layer containing polyvinylpyrrolidone and fatty acid as layer Moisturizes the skin of injured skin. Morphology and structure of nanofibers produced using various methods such as XRD X-ray spectroscopy, FTIR infrared Fourier transform spectroscopy, thermal weighing method or TGA, X-ray energy diffraction spectroscopy (EDAX-EDS), electron microscopy method (SEM), and finally the transmission electron microscopy (TEM) method was examined. The results of the instrumental analysis indicate the production of polymer fibers with a diameter in the range of 200-500 nanometers, which indicates the successful production of nanofibers, and also structural analyzes confirm the presence of silver nanoparticles and silver ions. The results of microbial tests show that nanofibers containing silver ions have a greater antimicrobial effect than silver nanoparticles. Manuscript profile -
Open Access Article
59 - Biosynthesis and characterization of silver nanoparticles using aqueous extract of walnut (Juglans regia L.) leaf
masoud bayat reza moradi ali bodaghiGreen synthesis of nanoparticles is an environmentally friendly method, in which water solvent are used. In this study, the biosynthesis of silver nanoparticles (Ag-NPs) was performed by using walnut (Juglans regia L.) leaf extract. Walnut leaf contains a variety of che MoreGreen synthesis of nanoparticles is an environmentally friendly method, in which water solvent are used. In this study, the biosynthesis of silver nanoparticles (Ag-NPs) was performed by using walnut (Juglans regia L.) leaf extract. Walnut leaf contains a variety of chemicals, including tannins, flavonoids, vitamins, plant acids and naphthoquinone. The extract of walnut (Juglans regia L.) leaf as the reducing agent is used for the synthesis of silver nanoparticles. The silver nanoparticles have been characterized by scanning electron microscopy (SEM), Energy dispersive X-ray (EDAX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and UV-Visible spectroscopy. UV-Vis spectrum exhibit an absorption band at around 400-450 nm suggestion the formation of silver nanoparticles. The observed peak at about 425 nm is related to the surface plasmon resonance of silver nanoparticles. The effects of operational parameters in the synthesis of silver nanoparticles such as silver nitrate salt concentration, extract volume, temperature and time was studied. The results showed that silver nitrate concentration 6 mM, extract volume 200 µlit, temperature 90°C and time 40 min was the optimum amounts. Manuscript profile -
Open Access Article
60 - Optimization of operating conditions and the role of ultrasound waves in the green synthesis of silver nanoparticles using apple tree leaf extract
Sarvin Mohammadi-Aghdam Omid AhmadiApple tree leaf extract is one of the effective materials in the synthesis of AgNPs, the results of FTIR spectroscopy showed the presence of several reducing agents. AgNPs were synthesized by mixing 2 ml of extract with 8 ml of AgNO3 solution based on the design of the MoreApple tree leaf extract is one of the effective materials in the synthesis of AgNPs, the results of FTIR spectroscopy showed the presence of several reducing agents. AgNPs were synthesized by mixing 2 ml of extract with 8 ml of AgNO3 solution based on the design of the experiment by surface response methodology in the heating temperature range of 30-70 °C and ultrasound time of 5-25 min. After the optimization, the results showed that the temperature of 52 °C and the duration of 15 min was the most suitable mode for the synthesis of AgNPs, in which the highest concentration was 27.10 ppm, the lowest average particle size was 66 nm, and the highest antioxidant property was 31.48. %. The results of UV-Vis spectroscopy and DLS analysis were confirmed at the optimum point with a slight difference, and the PDI and zeta potential were obtained as 0.397 and 21.2 mV, respectively. AgNPs showed good antibacterial properties against Staphylococcus aureus and Escherichia coli with halo diameters of 56 and 48 mm, respectively. The antifungal property of nanoparticles was 72%. Manuscript profile -
Open Access Article
61 - Effects of adding silver nanoparticles on thermal conductivity and viscosity of poly-alpha-olefin fluid
حمیدرضا قربانی سید کمیل حسینی اسفندانیIn this work, the silver nanoparticles effects on viscosity and thermal conductivity of silver-poly alpha olefin nanofluid were studied. Silver nanoparticles in different sizes and with various concentrations spread over the base fluid of poly alpha olefin and thermal c MoreIn this work, the silver nanoparticles effects on viscosity and thermal conductivity of silver-poly alpha olefin nanofluid were studied. Silver nanoparticles in different sizes and with various concentrations spread over the base fluid of poly alpha olefin and thermal conductivity were studied. Also, thermal conductivity was measured at different temperature conditions. In addition, the impact of nanoparticles on the viscosity of the fluid was measured at different concentrations. The thermal conductivity and viscosity of nanofluids increase with increasing volumetric concentration of silver nanoparticles. In addition, the thermal conductivity of nanofluid increases and the viscosity of nanofluid decreases with increasing temperature. Also, the thermal conductivity decreases with increasing nanoparticles size. Manuscript profile -
Open Access Article
62 - Synthesis and characterization of polyindole/silver nanoparticles nanocomposite and its application in the design of electrochemical sensor L-Cysteine
خدیجه قنبری زهرا بابایی سپیده بنیادیIn this study, an effective electrochemical sensor for the rapid measurement of L-cysteine based on glassy carbon electrode modified with polyindole/silver nanoparticles nanocomposite is presented. Polyindole nanofibers were synthesized by cyclic voltammetry method at t MoreIn this study, an effective electrochemical sensor for the rapid measurement of L-cysteine based on glassy carbon electrode modified with polyindole/silver nanoparticles nanocomposite is presented. Polyindole nanofibers were synthesized by cyclic voltammetry method at the glassy carbon electrode surface, and then silver nanoparticles were deposited on these nanofibers using a constant potential method. Surface morphology and characterization of the modified electrodes were confirmed by field emission scanning microscopy (FE-SEM), X-ray diffraction (XRD), Raman, and FT-IR spectroscopies. The electrochemical investigation showed that the polyindole/silver nanoparticles had very good efficiency with respect to the electrocatalytic oxidation of L-cysteine in phosphate buffer solution (pH = 7.0). The response of the glassy carbon electrode/polyindole/silver nanoparticles to L-cysteine was linear in the concentration range of 0.01-10 mM. The detection limit was obtained at signal/noise=3, 5.7 μM. In addition, the sensor showed good stability and repeatability. The application of the proposed sensor for the analysis of L-cysteine in human serum was successful. Manuscript profile -
Open Access Article
63 - Preparation of nylon 6,6 – Ag nanocomposite and characterization of its morphological and antibacterial properties
مریم فربودی ندا ایارهIn this research, silver nanoparticles were deposited on nylon 6,6 fibers with different methods including chemical, ultrasonic, and using colloidal silver solution. Also, the non-fiber nanocomposite of nylon 6,6-silver was prepared by dissolution of nylon 6,6 in formic MoreIn this research, silver nanoparticles were deposited on nylon 6,6 fibers with different methods including chemical, ultrasonic, and using colloidal silver solution. Also, the non-fiber nanocomposite of nylon 6,6-silver was prepared by dissolution of nylon 6,6 in formic acid in the presence of silver nanoparticles and extracted by acetone antisolvent. The morphological properties, antibacterial properties, elemental analysis, and wash durability of prepared samples were examined. SEM images and EDS analysis confirmed the presence of silver nanoparticles on nylon 6,6 in prepared samples before and after washing. The antibacterial capability of the samples were determined before and after washing against gram positive bacteria (S.aureus) and gram negative bacteria (E.coli) using resazurin color change. The obtained results confirmed, the sample prepared by ultrasonic method has the most effective antibacterial property before and after washing. According to the results of morphological observation and elemental analysis, it is due to the high amount and uniform deposition of silver on the fiber surface. Also, according to the results, non-fiber nylon6,6-silver nanocomposite has the weakest antibacterial property and other samples have intermediate properties. Manuscript profile -
Open Access Article
64 - Green synthesis of silver nanoparticle using Acroptilon repens extract and evaluation of its anti-efflux activity against Acinetobacter bumanni clinical isolates
Reyhane Behdad Amir Mirzaie Shohreh Zare KariziBackground & Objectives: Efflux pumps are one of the mechanisms for antibiotic resistance in Acinetobacter baumannii isolates. The aim of the present study was to investigate the green synthesis of silver nanoparticles using Acroptilon repens extract and evaluation MoreBackground & Objectives: Efflux pumps are one of the mechanisms for antibiotic resistance in Acinetobacter baumannii isolates. The aim of the present study was to investigate the green synthesis of silver nanoparticles using Acroptilon repens extract and evaluation of its anti- efflux activity in antibiotic-resistant A. baumannii isolates. Materials & Methods: In this experimental study, the silver nanoparticles were synthesized using A. repens alcoholic extract and the structure of the synthesized silver nanoparticles was confirmed by spectrophotometry (UV-Vis), Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Subsequently, the adeAC efflux pump was detected in 21 A. baumannii clinical isolates using Cartwheel and Polymerase Chain Reaction (PCR) methods. Finally, the anti-efflux pump activity of silver nanoparticles was evaluated by Minimum Inhibition Concentration (MIC) technique. Results: The synthesized silver nanoparticle was confirmed by maximum absorbance at 420 nm wavelength, using UV-Vis method. The SEM and TEM micrographs showed that nanoparticles are spherical and have an average size of 38.89 nm. Moreover, the XRD results confirmed the cubic structure of silver nanoparticles. The results of Cartwheel and PCR methods revealed that the 12 out of 21 isolates have active efflux pumps. Furthermore, the MIC of ethidium bromide in resistant strains with silver nanoparticles was decreased. Conclusion: Based on anti-efflux pump activity of silver nanoparticles against A. bumannii strains, it seems that silver nanoparticles have potential uses for pharmaceutical industries, though further studies are required to confirm the results of this study. Manuscript profile -
Open Access Article
65 - Antimicrobial effect of silver nanoparticles synthesized using weed seed exudates against the standard strains of Bacillus cereus and Acinetobacter baumannii
Mehrdad Khatami Shahram Pourseyedi Manoochehr Khatami Keighobad KeikavousiBackground & Objectives: Due to the antimicrobial ability of silver and increases in these ability at nano-levels, it is possible to use silver nanoparticles for treatment of infections. This study was aimed to evaluate antimicrobial effect of silver nanoparticles s MoreBackground & Objectives: Due to the antimicrobial ability of silver and increases in these ability at nano-levels, it is possible to use silver nanoparticles for treatment of infections. This study was aimed to evaluate antimicrobial effect of silver nanoparticles synthesized using weed seed exudates against of Bacillus cereus and Acinetobacter baumannii. Materials & Methods: In this sectional study, silver nanoparticles were synthesized using Prosopis farcta seed exudates and analyzed with UV-vis spectrophotometer, X-ray diffraction and transmission electron microscopy. The broth macro dilution method was used to investigate the antibacterial effects of silver nanoparticles. Results: Synthesis of silver nanoparticles was confirmed using UV-vis spectroscopy and X-ray diffraction analysis. Transmission electron microscopy showed the production of nanoparticles with 5-35 nm diameters. The minimum inhibitory concentrations against A. baumannii and B. cereus were 1.56 and 3.12 µg/mL and minimum bactericidal concentrations of 3.12 and 6.25 µg/mL, respectively. Conclusion: Biological synthesis of nanoparticles using P. farcta seed exudates is a very cost effective method and there is no need to energy sources. The ability to synthesize silver nanoparticles using P. farcta can make this agricultural-useless plant as a biological source for the synthesis of nanoparticles using. Manuscript profile -
Open Access Article
66 - Antibacterial effect of silver nanoparticles synthesized from the red algae Gracilaria gracilis
somayeh Ataei- e jaliseh maryam zamani kochesfehni mohammad hossein zamani kochesfehaniSilver nanoparticles (Ag-np) have high penetration and antimicrobial effect due to their high surface-to-volume ratio. The aim of this study was to biosynthesize silver nanoparticles with red algae extract, Gracilaria gracilis, and to investigate their MoreSilver nanoparticles (Ag-np) have high penetration and antimicrobial effect due to their high surface-to-volume ratio. The aim of this study was to biosynthesize silver nanoparticles with red algae extract, Gracilaria gracilis, and to investigate their antibacterial activity against a number of standard and drug-resistant pathogenic bacteria. First, Ag-np were synthesized. To confirm the structure and size of Ag-np, was used X-Ray diffraction spectroscopy, FE-SEM electron microscopy, and X-ray energy dispersive spectroscopy (EDS). The antimicrobial effects of algae extract on bacteria were determined by sequential dilution method. The size of nanoparticles under electron microscopy was between 12 and 46 nm. The nanoparticles were able to inhibit most of standard and antibiotic resistant bacteria, Ag-np at a concentration of 29 μg /ml, on the standard bacteria: S. typhimorium, E. coli, K. pneumonia and the clinically resistant bacteria, E. coli and K. pneumonia, they had the most inhibitory effect. In contrast, standard and clinically resistant isolates of S. aureus and standard strain S. pneumonia were resistant to Ag-np. The results of this research showed that the G. gracilis red algae as a bio-source that can be useful for green synthesis of silver nanoparticles at very low cost applications, these nanoparticles can be used as candidates for drug composition. Manuscript profile -
Open Access Article
67 - Effect of silver nanoparticles on the expression of pvl and tsst genes of Staphylococcus aureus in Real-time PCR
yasaman dastgir zahra keshtmand katayun borhanieBackground and objective: Nowadays, research on nanoparticles as antimicrobial compounds is increasing.Staphylococcus aureus is an important nosocomial pathogen that produces a wide range of exotoxins that are involved in causing disease in the host.The aim of this stud MoreBackground and objective: Nowadays, research on nanoparticles as antimicrobial compounds is increasing.Staphylococcus aureus is an important nosocomial pathogen that produces a wide range of exotoxins that are involved in causing disease in the host.The aim of this study was to investigate the effect of silver nanoparticles (AgNPs) on the expression of pathogenic genes of pvl and tsst genes in Staphylococcus aureus. Methods and methods: In this experimental study, the minimum growth inhibition concentration of silver nanoparticles was determined by Broth microdilution method. First, the lowest concentration of silver nanoparticles that inhibits bacterial growth was determined and then at a concentration lower than that, the expression of pvl and tsst genes in Staphylococcus aureus was studied by Real-Time PCR method. Data were analyzed using one-way ANOVA, Tukey test and P-value < 0.05. Results: Silver nanoparticles with concentration 62.5μg/ml had growth effect on Staphylococcus aureus bacteria. Also at concentration 31.25 μg/ml, the expression of pvl and tsst genes in Staphylococcus aureus was significantly reduced compared to the reference gene (rpo) (P <0.001). Conclusion: According to the results of the present study, silver nanoparticles can inhibit the growth of Staphylococcus aureus bacteria and reduce the expression of virulence genes pvl and tsst. Manuscript profile -
Open Access Article
68 - Synthesis of silver nanoparticles from Ephedra intermedia extract and evaluation of antibacterial and antioxidant properties
Mina Tetrontan Seyed Mohammad Mahdi Hamdi Maryam Ebrahimi TajabadiBackground & Objective: Ephedra intermedia species from the Ephedraceae family is a shrubby plant and is considered among the primitive plants. The aim of this study is to synthesize silver nanoparticles from the extract of this species in order to investigate its antim MoreBackground & Objective: Ephedra intermedia species from the Ephedraceae family is a shrubby plant and is considered among the primitive plants. The aim of this study is to synthesize silver nanoparticles from the extract of this species in order to investigate its antimicrobial and antioxidant effects. Materials and methods: First, methanolic extract was prepared and silver nanoparticles were synthesized using silver salt. A spectrophotometric device was used to verify silver nanoparticles and a scanning electron microscope was used to check its dimensions and shape. FTIR analysis was used to investigate the possible organic compounds involved in the synthesis of nanoparticles, and to determine the concentration of nanoparticles, the analysis was performed by AAS and the antioxidant properties were evaluated by the DPPH method. In order to evaluate the antimicrobial activity, MBC and MIC and disking method were used. Results: The nanoparticles produced were spherical and in the range of 30-89 nm, and the most effective group of agents that played a role in its production were the hydroxyl group (O-H) and alkene compounds (C=C), and the concentration of biosynthetic nanoparticles was 2.25 mg/liter indicates a high concentration of synthesized nanowires. The results of MIC and MBC tests were the same and its concentration was 2000 μg/ml. Conclusion: The results of this research showed that the biosynthetic nanoparticle obtained from Ephedra Intermedia is more effective in inhibiting the growth of bacteria than the commercial nanoparticle, so it can be used as an alternative in pharmaceutical, medical and disinfectant applications. Manuscript profile -
Open Access Article
69 - Study of ability Fusarium oxysporum fungi in extracellular biosynthesis of silver nanoparticle in vitro
Golshid Sajjadi Abolfath Shojaiee Mohammad Reza Fazeli Javid Amini Hossain JamalifarBackground and Objective: Development of methods without using plotant and poisonous material and minimum wastes to produce under control nano structures is of nanotechnologist concerns. In this way biologists, by previous knowledge, nano-scale minerals controlled makin MoreBackground and Objective: Development of methods without using plotant and poisonous material and minimum wastes to produce under control nano structures is of nanotechnologist concerns. In this way biologists, by previous knowledge, nano-scale minerals controlled making by microorganism, seeking organism capable making non-organic nanoparticles. The aim of this study is extracellular production of silver nanoparticles with maximum dimention of 20 nm by Fusarium oxysporum fungi. Materials and methods: After studies to optimize growth condition, Fusarium oxysporum biomass in a medium containing yeast and malt extract is reproduced. After silver nanoparticles production in silver nitrate solution, this nanoparticles are studies by UV-Visible Spectrophotometry and Transmission Electron Microscopy (TEM) methods. Results: Studies showed that when Fusarium oxysporum biomass put in 10-3 M of silver ions, can produce silver nanoparticles in the form of extracellular. Conclusion: Because of physical and chemical particular properties of silver nanoparticles with maximum diameter of 20 nm of Fusarium oxysporum fungi, its industrial production and applied evaluation is offered. Manuscript profile -
Open Access Article
70 - The Inhibitory Effect of Doxycycline- Silver Nanoparticle Conjugate on Brucella melitensis
Mohsen Ajalli Mojtaba Salouti Hamed Alizadeh Zahra Heydari Hossein Hamzehei Aram AlizadehBackground and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver MoreBackground and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver nanoparticles conjugate against Brucella meltensis 16M. Material and Methods: After preparing the doxycycline-silver nanoparticle conjugate, its antimicrobial activity against Brucella meltensis 16M was determined by Well Diffusion Agar method in Muller Hintone Agar media. Also, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Doxycycline-silver nanoparticle conjugate was determined by Macrodilution method in Muller Hintone Broth media. Finally, antibacterial effect of the nanoparticle was assayed in animal model. Results: The results showed that Doxycycline-silver nanoparticle conjugate has antimicrobial activity against Brucella melitensis 16M in laboratory condition. In mouse model, the conjugate of Doxycycline-silver nanoparticle could decrease effectively the Brucella melitensis load in liver. Conclusion: This study demonstrated that Doxycycline-silver nanoparticle conjugate has synergistic effect on Brucella melitensis 16M and can be useful in treatment of brucellosis. Manuscript profile -
Open Access Article
71 - Anti-microbial Effect of Nanoparticles on Non-Helicobacter Pylori Urease Positive Bacteria Isolated from Peptic Ulcer Patients
Jamile Nowrozi Mahtab Golmohamadi Ghadikolaii Farzane Hosaini Shahram Agah Siamak KhaleghiBackground and Objective: Recently the presence of several urease-positive bacteria other than Helicobacter pylori has been reported in gastric ulcer patients. The purpose of this study was the isolation and identification of urease-positive bacteria other than Helicoba MoreBackground and Objective: Recently the presence of several urease-positive bacteria other than Helicobacter pylori has been reported in gastric ulcer patients. The purpose of this study was the isolation and identification of urease-positive bacteria other than Helicobacter pylori in patients with gastric ulcer and at the same time, determining the anti-microbial effects of silver nanoparticles on the isolated bacteria. Materials and Methods: 50 gastric antrum biopsies were collected from patients with gastric ulcer who were admitted to the Rasoul Akram hospital (Tehran) by gastrointestinal specialists. The samples were transferred to the microbiology laboratory by transitive liquid medium. Urease-positive bacteria in the stomach were identified by standard bacteriological methods, including culture-specific and biochemical tests. The antimicrobial effects of the silver nanoparticles on urease-positive bacteria were determined according to minimum inhibitory concentration (MIC) and Minimum bactericidal Concentration (MBC) techniques. Results: The results showed that 42% of collected samples was urease-positive (10% Klebsiella pneumoniae, 10% Staphylococcus aureus, 8% Enterobacter cloace, 6% Enterobacter aglomerans, 4% Klebsiella azaene and 4%Citrobacter frondi). The antimicrobial effects of silver nanoparticles on the isolated bacteria showed 1.56-12.5 MIC and 3.125-25 MBC. Conclusion: Growth of urease-positive bacteria may lead to false positive observation on UBT and rapid urease tests. Therefore, it is better all urease-positive bacteria isolated from stomach to be sent for accurate diagnosis in order to improve the impacts of treatment. Also, in order to avoiding of bacterial resistance to antibiotics, silver nanoparticles are appropriate alternatives. Manuscript profile -
Open Access Article
72 - بررسی کاربرد نانو ذرات نقره بیوسنتز شده با استفاده از عصاره گیاه دارویی زنیان (Trachyspermum ammi) در کنترل پوسیدگی باکتریایی پیاز
زینب فتوحیانامروزه استفاده از نانوذرات فلزی به عنوان جایگزین مناسب برای سموم شیمیایی خطر آنها را کاهش میدهد. لذا هدف از مطالعه حاضر بیوسنتز نانوذرات نقره با استفاده از عصاره آبی دانه زنیان بومی منطقه و بررسی اثر آن بر علیه باکتری پوسیدگی پیاز میباشد. در این پژوهش ابتدا جداسازی و Moreامروزه استفاده از نانوذرات فلزی به عنوان جایگزین مناسب برای سموم شیمیایی خطر آنها را کاهش میدهد. لذا هدف از مطالعه حاضر بیوسنتز نانوذرات نقره با استفاده از عصاره آبی دانه زنیان بومی منطقه و بررسی اثر آن بر علیه باکتری پوسیدگی پیاز میباشد. در این پژوهش ابتدا جداسازی و شناسایی باکتری عامل بیماری از مناطق مختلف شهرستان جیرفت انجام گردید. آنگاه بیوسنتز نانوذرات نقره توسط عصاره آبی دانه زنیان و اثر آنها علیه پکتوباکتریوم در شرایط آزمایشگاه و انبار بررسی شد. مجموعاً 12 ایزوله باکتری جداسازی و ایزوله N3از منطقه عنبرآباد دارای بیشترین شدت بیماریزایی بود. بیوسنتز نانوذرات نقره توسط عصاره با مشاهده رنگ خرمایی بعد از گذشت 15 دقیقه در شرایط تاریکی تایید گردید و بررسی طیف سنج ماورابنفش مشخص نمود که پلاسمون رزونانس سطحی در طول موج 420 نانومتر است. میزان بازدارندگی از رشد نانوذرات نقره بیوسنتز شده و سم مانکوزب هر کدام به تنهایی و ترکیب آنها علیه پکتوباکتریوم در شرایط آزمایشگاه به ترتیب 62.45، 100 و88.67 درصد بود. همچنین نانوذرات نقره و سم مانکوزب هر کدام به تنهایی طی سه ماه و تلفیق آنها با هم طی دو ماه به میزان100 درصد باعث ماندگاری پیاز در انبار شدند. اما تلفیق نانوذرات نقره و سم مانکوزب به همراه پکتوباکتریوم در ماه اول و دوم، 100 درصد و در ماه سوم، 85 درصد علیه بیماری پوسیدگی پیاز بازدارندگی داشتند. بنابراین نتایج نشان داد که نانوذرات نقره سنتز شده بر کنترل باکتری بیمارگر در شرایط آزمایشگاه و انبار موثر بودند و میتوانند جایگزین سموم کشاورزی شوند. Manuscript profile -
Open Access Article
73 - کاربرد بیوسنتز نانوذرات نقره با استفاده از عصاره تراکی اسپرم در کنترل پوسیدگی باکتریایی پیاز
زینب فتوحیانامروزه استفاده از نانوذرات فلزی به عنوان جایگزینی مناسب برای سموم شیمیایی خطر آن را برای محیط زیست و مصرف کنندگان کاهش می دهد. بنابراین، هدف از این مطالعه بیوسنتز نانوذرات نقره با استفاده از عصاره آبی دانههای عجوان و تأثیر آن بر پوسیدگی باکتریایی پیاز است. در این تحقیق Moreامروزه استفاده از نانوذرات فلزی به عنوان جایگزینی مناسب برای سموم شیمیایی خطر آن را برای محیط زیست و مصرف کنندگان کاهش می دهد. بنابراین، هدف از این مطالعه بیوسنتز نانوذرات نقره با استفاده از عصاره آبی دانههای عجوان و تأثیر آن بر پوسیدگی باکتریایی پیاز است. در این تحقیق ابتدا جداسازی و شناسایی باکتری های بیماری زا از مناطق مختلف شهرستان جیرفت انجام شد. سپس بیوسنتز نانوذرات نقره توسط عصاره آبی دانه عجوان انجام شد و اثرات آنها بر روی پکتوباکتریوم در شرایط آزمایشگاهی و انبار بررسی شد. در مجموع 12 جدایه باکتریایی، جدایه N3 از منطقه عنبرآباد بیشترین بیماری زایی را داشت. بیوسنتز نانوذرات نقره با مشاهده رنگ تاریخ پس از 15 دقیقه در تاریکی در دمای اتاق تایید شد و طیف سنج فرابنفش نشان داد که تشدید پلاسمون سطحی در 420 نانومتر است. نرخ مهار رشد نانوذرات نقره بیوسنتز شده و سم Mancozeb هر کدام به تنهایی و ترکیب آنها در برابر پکتوباکتریوم در شرایط آزمایشگاهی به ترتیب 45/62، 100 و 67/88 درصد بود. همچنین، نانوذرات Ag و Mancozeb هر کدام به تنهایی در مدت سه ماه و ترکیب آنها با یکدیگر در طی دو ماه باعث ماندگاری 100 درصدی پیاز در انبار شد. اما ترکیب نانوذرات نقره بیوسنتز شده و مانکوزب همراه با پکتوباکتریوم در ماه اول و دوم 100 درصد و در ماه سوم 85 درصد در برابر بیماری پوسیدگی باکتریایی مهار شد. بنابراین، نتایج نشان داد که نانوذرات نقره بیوسنتز شده در کنترل باکتری های بیماری زا موثر بوده و می توانند جایگزین سموم کشاورزی شوند. Manuscript profile -
Open Access Article
74 - Silvernano Particle Loaded on Activated Carbon as Novel Adsorbent for the Removal of Acid Yellow 199 Dye
Z. Alishavandi N. Mosallanejad R. shabani -
Open Access Article
75 - Green Synthesis of Silver Nanoparticles Using Pimpinella anisum L. Seed Aqueous Extract and Its Antioxidant Activity
Hashem Akhlaghi Sedighe sadat Akhlaghi -
Open Access Article
76 - The Possible Protective Role of Vitamin E on the Induced Silver Nanoparticles Toxicity on Filiform and Circumvallate Tongue Papillae of Albino Rats Histological and Immunohistochemical Study
Nada Tarek Zaki Mahmoud Mohamed Al Ankily Reham Magdy Amin Ahmed Mahmoud Halawa -
Open Access Article
77 - Antimicrobial Potentials of Iron Oxide and Silver Nanoparticles Green-Synthesized in Fusarium solani
Masoomeh Sasani Ebrahim Fataei Reza Safari Fatemeh Nasehi Marzieh Mosayyebi -
Open Access Article
78 - Synthesis and Characterization of polyurethane/silver nanocomposite by Microwave assisted
seyed mohamad kazemzadeh Mohamad Reza Vaezi Seyyed Mohammad Mehdi Hadavi Masoud EsfandehIn this study, the dielectric heating was performed by microwave irradition to synthesize polyurethane/silver nanocomposite, which was carried out by distributing and dissolving silver nitrate salt in the polyurethane and the reduction of silver cation retained in ethyl MoreIn this study, the dielectric heating was performed by microwave irradition to synthesize polyurethane/silver nanocomposite, which was carried out by distributing and dissolving silver nitrate salt in the polyurethane and the reduction of silver cation retained in ethylene glycol micelles in the prepolymer solutions. Decreasing the time and number of synthesis processes and, consequently, reducing production costs is one of the advantages of this technique. During the chemical reactions, microwave waves with constant intensity and frequency at 30, 60, 90, 120 seconds were applied to solvent prepolymer by a microwave oven. By completion of the chemical reactions, silver nanoparticles were synthesized in a polyurethane matrix. The effect of microwave and insitu synthesis of and silver nanoparticles on chemical bonding of polyurethane in terms of structure, morphology, composition and particle size distribution were studied by using UV-Vis spectroscopy, X-ray diffraction (XRD), transmitted electron microscopy (TEM), X-ray diffraction spectroscopy (EDX) and Fourier transform infrared spectroscopy (FTIR) analysis. Produced nanoparticles have spherical and semi spherical morphology with particle size distribution in the range of 10 to 80 nm, and the fcc crystalline structure and uniform dispersion in the polyurethane matrix. Meanwhile, Fourier transform infrared spectroscopy of nanocomposites showed synthesis of silver nanoparticles no negative effect on polyurethane chemical bonds by this method. Manuscript profile -
Open Access Article
79 - Fabrication and Optimization of Humidity Sensor Based on Porous Nanostructure Tungsten Trioxide / Silver Nanoparticles
Ayub Karimzad Ghavidel Gholamreza Kiani Mahsa MahdaviniaThe aim of this research is to fabricate a moisture sensor based on porous nanostructure of pure tungsten trioxide (WO3) and doped with silver nanoparticles (SN), and to use the properties of ohmic changes, capacitance and impedance versus moisture as output. The struct MoreThe aim of this research is to fabricate a moisture sensor based on porous nanostructure of pure tungsten trioxide (WO3) and doped with silver nanoparticles (SN), and to use the properties of ohmic changes, capacitance and impedance versus moisture as output. The structural characteristics of the prepared nanocomposite were examined by a scanning electron microscope and X-ray diffraction spectroscopy. The results showed that synthetic WO3 is in the form of spheres with dimensions of 3 to 7 μm, with a surface porous structure in size of 10 to 60 nm. To fabricate the sensors, first the materials were mixed and dispersed, then coated on the interdigitated electrode by sedimentation method. The investigation showed that the doped sensor with SN have a better performance in comparison with WO3 sample. Its sensitivity was obtained 4.4 and 0.68 MΩ/%RH for the relative humidity range of 20-50% and 50-90%, respectively, improved 7 and 5% compared to similar previous samples. The response and recovery time of this sensor was measured approximately 2 and 7 seconds, which is 37.5 and 41.4 times smaller than available samples, respectively. Decreasing the capacitance of the sensor to 3800 Pf by increasing the relative humidity to 90% is another finding. Significant impedance changes of the sensor versus the humidity alternations is other important result, showing that the impedance can be considered as a sensor output by compensating for errors of ohmic changes and capacitance. Manuscript profile -
Open Access Article
80 - Histopathology and Anti-cancer Effects of Silver Nanoparticles on the Gelatin Substrate in Ovarian Cancer Induced by DMBA in Female Wistar Rats
عباسعلی دهپور جویباری مستانه میرمیرانی سیده مرضیه محمدی مرجان فرهمندOvarian cancer is one of the commonest cancers among the woman. Although not common, but one of the main causes of death in woman is considered.one of the main problem in the treatment of cancer is the lack of active drugs and effective drugs, and drug resistance to the MoreOvarian cancer is one of the commonest cancers among the woman. Although not common, but one of the main causes of death in woman is considered.one of the main problem in the treatment of cancer is the lack of active drugs and effective drugs, and drug resistance to the drugs available. Nanoparticles are one of the most well-known nanomaterials that have been studied for their anticancer properties. Among these nanoparticles, silver nanoparticles having very strong antimicrobial properties in medical and dental acceptility are high. Thus, the present study was carried out to study the cytotoxic effects of silver nanoparticles on the glycine substrate on ovarian cancer induced by DMBA in female wistar rats. In this study, female wistar rats were used .Animals were divided into 6 groups. The first group was the control group. The second group only injected by DMBA respectively. Third group treated with injection of DMBA+Salin and other groups treated injection of different doses of silver nanoparticles on the glycine substrates to induced cancer,7,12 dimethyl benza anthracen was directly injected in to ovary. In this study the weight of ovaries and ovarian histopathological features in all experimental groups were measured and evaluated and finally, data collected using 16 spss software and one way ANOVA were analyzed. The tumor weight in the group receiving the nanoparticle fell sharply. In fact, the silver nanoparticles were significantly positive effects on cancer cells. The microscopic section of luteinizing and follicle improved in the group receiving the silver nanoparticles on the glycine substrate was observed. This study confirms the positive effect of the silver nanoparticle on glycine substrate in treating the ovary cancer and destroying cancer cells, although more studies are needed. Manuscript profile -
Open Access Article
81 - The Effect of Silver Nanoparticles on the Viability of Lung Fibroblast Cell Line (MRC-5)
Yasaman Dastgir Zahra Keshtmand Katayoun BorhaniSilver nanoparticles (AgNPs) with very strong antimicrobial and anti-proliferative properties are widely used in medical sciences. However, little is known about their effect on healthy cells, and there were the reports of their toxicity. This study aimed to investigate MoreSilver nanoparticles (AgNPs) with very strong antimicrobial and anti-proliferative properties are widely used in medical sciences. However, little is known about their effect on healthy cells, and there were the reports of their toxicity. This study aimed to investigate the effect of silver nanoparticles on the viability rate of the MRC-5 cell line. In this experimental study, during 24, 48, and 72 hours, different concentrations of silver nanoparticles (1.56, 3.125, 6.5, 12.5, 25, 50, and 100 μg/ml) per category MRC-5 cells were examined by MTT assay. Data were analysed using SPSS software, one-way ANOVA, Tukey test, and a significant level of p < /em> < 0.05. The treatment of normal MRC-5 cells with different concentrations of silver nanoparticles after 24, 48, and 72 hours by MTT method showed a significant reduction in the viability of cells at concentrations of 25, 50, and 100 μg/ml (p < /em> < 0.05). The results of this study showed that silver nanoparticles have a dose-dependent inhibitory effect on normal MRC-5 cells. Manuscript profile -
Open Access Article
82 - Effects of Ovo Injection of Silver Nanoparticles on Immune System Response in Broiler Chickens
Samira Arabameri Firooz Samadi Behrooz Dastar Zarbakht Ansari Reza MobaseriIn this study, the effect of ovo injection of silver nanoparticles on the immune system response of broilers was studied. In this regard, 560 eggs were randomly divided into four groups with four replications. Experimental groups included: 1) positive control (injection MoreIn this study, the effect of ovo injection of silver nanoparticles on the immune system response of broilers was studied. In this regard, 560 eggs were randomly divided into four groups with four replications. Experimental groups included: 1) positive control (injection of 1 mL of physiological serum on the seventh day of incubation), 2) negative control (no injection of physiological serum), 3) injection of 20 mg silver nanoparticles, and 4) injection of 40 mg silver nanoparticles per it was an egg. The injection of silver nanoparticles and the control group was performed on day 7 of the incubation period. At the end of the incubation period, the chicks were counted to check the percentage of hatchability and after weighting, they were slaughtered to check the parameters of the immune system. There was a significant difference in hatching percentage in experimental groups (p <0.05) so that the negative control group showed the highest percentage of hatching chickens. Silver nanoparticles improved the growth of chickens during the incubation period (p <0.05) so that the group receiving 20 mg of silver nanoparticles showed the highest body weight. Silver nanoparticles significantly increased the relative weight of liver and spleen (p <0.05). Immunoglobulin G (IgG, M (IgM) concentration, total white blood cell count, and heterophile to lymphocyte ratio did not show significant differences (p >0.05). Hepatic tumor necrosis factor-alpha (TNF-α) gene expression Interleukin (6) (IL-, beta-modifying growth factor (TGF-β)) and insulin-like growth factor-1 (IGF-1) showed significant differences in the experimental groups (p <0.05). The group receiving 20 mg of silver nanoparticles showed the highest level of gene expression. In general, the results of this study showed that ovo injection of silver nanoparticles strengthens the immune system of broilers. Manuscript profile -
Open Access Article
83 - Compare the Toxicity of Silver Nanoparticles Produced by Biological and Chemical Methods on Artemia franciscana in Naupliar and Adult Stages
S. Mashjoor M. Alishahi Z. Tulaby DezfulyIn this study, the toxicity of silver nanoparticles (AgNPs) and the difference in sensitivities in naupliar and adult stages of Artemia fransiscana, were evaluated to absorption of chemical and biosynthesized by the seaweed Ulva flexuosa, forms of AgNPs. The A. nauplii MoreIn this study, the toxicity of silver nanoparticles (AgNPs) and the difference in sensitivities in naupliar and adult stages of Artemia fransiscana, were evaluated to absorption of chemical and biosynthesized by the seaweed Ulva flexuosa, forms of AgNPs. The A. nauplii and adult were exposed to additive serial concentration of biosynthetic and chemical AgNPs. Mortality in each group at the times of 12, 24, 36 and 48 h after exposure to AgNPs were recorded and analyzed via Probit software. The toxicity of these two types of AgNPs on A. nauplii and adult increased in a dose-dependent manner, but also their toxicity increased along with duration of exposure time. The toxicity of nanoparticles were significantly different (P<0.05). Therefore, after 48 h, the LC50 value of chemical AgNPs in the A. nauplii was 31.8 mg/l, while 48 h LC50 for biosynthesis AgNPs was 366.96 mg/l. While the concentration of the chemical AgNPs have assessed in adults artemia during exposure was 47 mg/l and for biosynthesis form of AgNPs was 240 mg/l. The chemical form of AgNPs was more toxic potential than a biosynthetic form of its. The sensitivity of A. nauplii to chemical AgNPs was more than to mature stage, but compared to biosynthesis form was less than adult. This research could be pioneer to the development of biocompatible functionality of nanoparticles with eco-management objectives. Manuscript profile -
Open Access Article
84 - Effects of Silver Nanoparticles on Oxidative Stress in Adult Female Wistar Rats
N. Nowrozi E. Samani Jahromi S. Zolghadri JahromiWe aimed to evaluate the antioxidant effect of silver nanoparticles on oxidative stress in adult female of Wistar rats. In this experimental study, 50 adult female of Wistar rats were randomly divided into 5 groups. The control group (no treatment), sham group (0.2 mg/k MoreWe aimed to evaluate the antioxidant effect of silver nanoparticles on oxidative stress in adult female of Wistar rats. In this experimental study, 50 adult female of Wistar rats were randomly divided into 5 groups. The control group (no treatment), sham group (0.2 mg/kg physiology serum), experimental group 1 (50 mg/kg isoniazid), experimental group 2 (50 mg/kg isoniazid and 0.25 mg/kg silver nanoparticles) and the experimental group 3 (50 mg/kg isoniazid and 0.5 mg/kg silver nanoparticles) were given. All injections were prescribed for 15 d and isoniazid and silver nanoparticles were injected in gavage and intraperitoneally, respectively. Then all animals were anesthetized and blooded. In the end, the data were examined by ANOVA test at a significance level of P<0.05 using SPSS software. Isoniazid increases nitric oxide, reduces glutathione peroxidase activity and total antioxidant capacity. While treating the animals with silver nanoparticles reduced the side effects of oxidant-induced by isoniazid, with decreasing nitric oxide, increased glutathione peroxidase and total antioxidant capacity by reducing nitric oxide production and increasing glutathione peroxidase activity oxidative, silver nanoparticle prevent from oxidative damages and the destruction of red blood cell (RBC) membrane. In other words, the tests showed the antioxidant role of silver nanoparticles as well. Manuscript profile -
Open Access Article
85 - Survey of silver nanoparticles (Ag-NPs) effects on blood indices of Rainbow trout (Oncorhynchus mykiss) juveniles
Mina Tavassoli Rudsari Hamidreza Jamalzadeh Elmira Mikaeili Agah -
Open Access Article
86 - Check effect of Silver nano Colloid and Humic acid on the agronomic aspect of fodder beet
rasoul goli kalaninvestigate the effect of silver Nanoparticle and humic acid on agronomic aspect of fodder beet, an experiment as factorial form in the complete randomized block design in three replications in 2019 was conducted experimented factors were included nano application at t Moreinvestigate the effect of silver Nanoparticle and humic acid on agronomic aspect of fodder beet, an experiment as factorial form in the complete randomized block design in three replications in 2019 was conducted experimented factors were included nano application at three levels without treatment.100 and 200 ml per 300 liters of water as a first factor and humic at three levels of control non-using, 25 and 50 liters per hectare as irrigation fertilizer as second factor were considered. The most wet and dry yield of forage beet were obtained in the amount of77.2 and14.6 tons per hectare, in the 200 ml of silver Nanoparticle foliar application treatment and using of 50 lit/ha of humic acid were obtained, however, a statistically significant difference with 100 ml Nanoparticle and 25 ml humic acid did not observed. most leaf area index and root diameter was observed in 200 ml of r nanoparticle treatment and the using of 25 humic The using of nanoparticle was increased the protein and raw fiber, ability to digest dry matter, and the amount of auxin. other hand, the most percent of sugar was recorded in the non-using of humic acid treatment, but the amount of root potassium, the protein percent and raw fiber, digestibility and the amount of auxin by humic using compared to control treatment that showed a significant increasing. Based the experiment results, the humic using in amount of 25and 100of nanoparticle application increasing of forage beet quantitative and qualitative performance are effective. Manuscript profile -
Open Access Article
87 - Biosynthesis of Silver Nanoparticles using Bacillus subtilis Bacterium Cultured in Corn Steep Liquor and Evaluation of its Antibacterial Activity
Naeimeh Faridi Aghdam Shahram Moradi Dehaghi Sirous Ebrahimi Hamed HamshehkarIn this study, the biosynthesis of silver nanoparticles was done using Bacillus subtilis bacterium cultured in corn steep liquor (CSL) nutrient. The biosynthesized nanoparticles were characterized by several techniques including FT-IR, XRD, UV-Vis, SEM, EDX, and TEM. MoreIn this study, the biosynthesis of silver nanoparticles was done using Bacillus subtilis bacterium cultured in corn steep liquor (CSL) nutrient. The biosynthesized nanoparticles were characterized by several techniques including FT-IR, XRD, UV-Vis, SEM, EDX, and TEM. The absorption spectrum of the nanoparticles indicated the maximum absorption at 436 nm. The SEM image confirmed the nanoparticles had polydisperse spherical morphology (~20nm). Also, the TEM image showed the nanoparticles had spherical or elliptical shape and the approximate diameter of the particles was between 10-20 nm. Morphological studies showed that the nanoparticles were completely separated and no aggregation was observed. Moreover, XRD studies confirmed that the produced nanoparticles were crystallized in the FCC crystal lattice. The antibacterial activity results indicated that the synthesized nanoparticles had significant effect against Escherichia coli bacteria, and the inhibition zone was equal to Gentamicin. So, the production of silver nanoparticles using green method is economically very economical, and can be a method for the production of silver nanoparticles in industrial scale. Manuscript profile -
Open Access Article
88 - Phytosynthesis of silver nanoparticles using Otostegia persica
halime gholami ebrahim molashahi fateme sargezy alireza aeinaliIn this study we researched about the synthesis of silver nanoparticle by the aqueous extract of Otostegia persica. In the first the results of HPLC was showed extracts of Otostegia persica contains poly phenols compound such as sinapic acid, caffeic acid, trans-feralic MoreIn this study we researched about the synthesis of silver nanoparticle by the aqueous extract of Otostegia persica. In the first the results of HPLC was showed extracts of Otostegia persica contains poly phenols compound such as sinapic acid, caffeic acid, trans-feralic, gallic acid, p-coumaric acid and vanillin that sinapic acid, caffeic acid, trans-feralic are significant compound. Therefore, in this research, we have attempted to synthesize nanoparticles by using the aqueous extract of the Otostegia persica, which contains compounds such as sinapic acid, caffeic acid, trans-feralic acid, gallic acid, p-coumaric acid and vanillin. In this study, it was found that the aqueous extract of Otostegia persica is able to synthesis silver nanoparticles. In this research, after the reaction of solutions with each other, the first step was the color change from green to brown. Based on previous research, the reaction mixture changes color by adding different concentrations of metal ions. These color changes are caused by the stimulation of surface plasmon vibrations in silver nanoparticles, so the change in the color of the reactive mixture indicates the synthesis of silver nanoparticles. The aqueous extract of Otostegia persica was used as a stabilizer or capping agent and reducing agent to reduce Ag+ to metallic silver. The phenolic compounds in the Otostegia persica extract have the ability to react with silver ions in silver nitrate solution and exchange electrons and reduce silver ion (Ag+) to neutral silver (Ag0) in nano dimensions. Then we tried to optimize the research and the various components were optimization such as the time, pH, the concentration of extract and silver nitrate. Finally, the volume of the extract was analyzed and by using UV device, we proved the formation of silver nanoparticles and was observed a peak in the area of 734 nm. The results were showed that in the concentration of 1 mM from silver nitrate and the temperature of 22 C and pH=8 are the optimal conditions for the formation of silver nanoparticles. Then were sampled from the silver nanoparticles, the average size of silver particles and the structure of nanoparticles were determined by using TEM duvice and the size of nanoparticle it was found 34 nm and its shape was observed as a sphere. Then we using FTIR and XRD for proved the formation of silver nanoparticles and we proved the existence of silver nanoparticles. Therefore, in this study, it was found that using polyphenolic compounds in the aqueous extract of Otostegia persica as a natural, harmless, inexpensive and with antioxidant and reducing properties can be used for the synthesis of metal nanoparticles, including silver nanoparticles that these silver nanoparticles are very important in the medical and pharmaceutical industries. The general conclusion from this research is the high value and importance of medicinal plants in the green synthesis of nanoparticle, which is a suitable alternative instead of using harmful chemicals, including the Otostegia persica, which has the ability to be planted and harvested in areas has different varieties of Iran and the plant is rich in secondary compounds, including polyphenolic compounds with high reductive and antioxidant properties, which is a suitable option for synthesis nanoparticles with appropriate shape and size. Manuscript profile
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