Study of ability Fusarium oxysporum fungi in extracellular biosynthesis of silver nanoparticle in vitro

Subject Areas : Microbial Biotechnology

Golshid Sajjadi ¹ , Abolfath Shojaiee ² , Mohammad Reza Fazeli ³ , Javid Amini ⁴ , Hossain Jamalifar ⁵

1 - Department of Microbiology, Islamic Azad University, Lahijan branch, Lahijan, Iran
2 - Department of Nutrition, Iran University of Medical sciences, Tehran, Iran
3 - Department of Microbiology, School of Pharmacy, Tehran University, Tehran, Iran
4 - Department of Microbiology, Education Researcher Center, Kerman, Iran
5 - Department of Microbiology, School of Pharmacy, Tehran University, Tehran, Iran

Received: 2009-04-14 Accepted : 2009-05-15 Published : 2009-04-01

Keywords: Silver nanoparticles, Extracellular, Fusarium oxysporum fungi, Laboratory Scale,

Abstract :

Background and Objective: Development of methods without using plotant and poisonous material and minimum wastes to produce under control nano structures is of nanotechnologist concerns. In this way biologists, by previous knowledge, nano-scale minerals controlled making by microorganism, seeking organism capable making non-organic nanoparticles. The aim of this study is extracellular production of silver nanoparticles with maximum dimention of 20 nm by Fusarium oxysporum fungi. Materials and methods: After studies to optimize growth condition, Fusarium oxysporum biomass in a medium containing yeast and malt extract is reproduced. After silver nanoparticles production in silver nitrate solution, this nanoparticles are studies by UV-Visible Spectrophotometry and Transmission Electron Microscopy (TEM) methods. Results: Studies showed that when Fusarium oxysporum biomass put in 10-3 M of silver ions, can produce silver nanoparticles in the form of extracellular. Conclusion: Because of physical and chemical particular properties of silver nanoparticles with maximum diameter of 20 nm of Fusarium oxysporum fungi, its industrial production and applied evaluation is offered.