The Effect of Silver Nanoparticles on the Viability of Lung Fibroblast Cell Line (MRC-5)

Subject Areas : Journal of Animal Biology

Yasaman Dastgir ¹ , Zahra Keshtmand ² , Katayoun Borhani ³

1 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Department of Environment and Food Industry Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2020-08-05 Accepted : 2020-10-25 Published : 2021-02-19

Keywords: viability, silver nanoparticles, MTT, MRC-5,

Abstract :

Silver nanoparticles (AgNPs) with very strong antimicrobial and anti-proliferative properties are widely used in medical sciences. However, little is known about their effect on healthy cells, and there were the reports of their toxicity. This study aimed to investigate the effect of silver nanoparticles on the viability rate of the MRC-5 cell line. In this experimental study, during 24, 48, and 72 hours, different concentrations of silver nanoparticles (1.56, 3.125, 6.5, 12.5, 25, 50, and 100 μg/ml) per category MRC-5 cells were examined by MTT assay. Data were analysed using SPSS software, one-way ANOVA, Tukey test, and a significant level of p < /em> < 0.05. The treatment of normal MRC-5 cells with different concentrations of silver nanoparticles after 24, 48, and 72 hours by MTT method showed a significant reduction in the viability of cells at concentrations of 25, 50, and 100 μg/ml (p < /em> < 0.05). The results of this study showed that silver nanoparticles have a dose-dependent inhibitory effect on normal MRC-5 cells.

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