پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی
Subject Areas : Camelا. عباسی 1 , س.ر. هاشمی 2 , س. حسنی 3 , م. ابراهیمی 4
1 - Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
2 - Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
3 - Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
4 - Department of Food Industry, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
Keywords: عملکرد, تنش گرمایی, دستگاه گوارش, اسید آلی, نانو ذرات نقره,
Abstract :
هدف از این آزمایش ارزیابی پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی میباشد. به همین منظور در این آزمایش از 375 قطعه جوجه گوشتی یکروزه نژاد کاب (500 Cobb) استفاده شد. که در 5 تیمار و 5 تکرار و 15 قطعه جوجه در هر تکرار در قالب یک طرح کاملاً تصادفی تقسیم شدند. پنج تیمار غذایی شامل: 1) جیره شاهد، 2) جیره شاهد حاوی 1 درصد زئولیت، 3) جیره شاهد حاوی 1 درصد زئولیت پوشش داده شده با 0.5 درصد نانو نقره، 4) جیره شاهد حاوی 1 گرم بر کیلوگرم اسید آلی و 5) جیره شاهد حاوی 1 درصد زئولیت پوشش داده شده با 0.5 درصد نانو نقره و 1 گرم بر کیلوگرم اسید آلی بود. خوراک مصرفی و وزن بدن برای هر تیمار در کل دوره آزمایش ثبت و ضریب تبدیل خوراک محاسبه گردید. به منظور شمارش جمعیت میکروبی دستگاه گوارش دو قطعه جوجه از هر واحد آزمایشی در روزهای 21 و 42 پرورش کشتار شدند. نتایج آزمایش نشان داد که جیره غذایی حاوی نانو نقره به همراه اسید آلی باعث کاهش وزن بدن جوجههای گوشتی در مقایسه با تیمار شاهد و زئولیت در فاصله سن 42-21 روزگی شد (P<0.05). همچنین در کل دوره پرورش ضریب تبدیل غذایی در گروه نانو نقره پوشش داده شده بر زئولیت به طور معنی داری نسبت به گروه شاهد و زئولیت بالاتر بود (P<0.05). با این حال تیمارهای آزمایشی هیچگونه اختلاف معنیداری بر جمعیت میکروبی دستگاه گوارش نداشتند (0.05<P). به طور خلاصه نتایج حاضر نشان داد اگرچه نانو ذرات نقره و اسیدهای آلی اثر خاصی بر پارامترهای عملکرد و افزایش شمار باکتریهای مفید (اسید لاکتیک) دستگاه گوارش در حالت تنش گرمایی نداشتند اما موجب کاهش آنها نگشتهاند. بنابراین میتوان از آنها به عنوان افزودنی در جیره غذایی جوجههای گوشتی استفاده نمود.
Abdel-Azeem F., El-Hommosany Y.M. and Nematallah G.M. (2000). Effect of citric acid in diets with different starch and fiber levels on productive performance and some physiological traits of growing rabbits. Egyptian J. Rabbit Sci. 10, 121-145.
Abdel-Fattah S., Sanhoury A., Mednay N. and Abdel-Azim F. (2008). Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. Int. J. Poult. Sci. 7, 215-222.
Abdo M. and Zeinb A. (2004). Efficacy of acetic acid in improving the utilization of low protein-low energy broiler diets. Egypt. Poult. Sci. 24, 123-141.
Ahmadi F. and Rahimi F. (2011). Factors affecting quality and quantity of egg production in laying hens: A review. World Appl. Sci. J. 12, 372-384.
Ahmadi F., MohammadiKhah M., Saman J., Zarneshan A., Akradi L. and Salehifar P. (2013). The effect of dietary silver nanoparticles on performance, immune organs, and lipid serum of broiler chickens during starter period. Int. J. Biosci. 3, 95-100.
Ahmadi J. (2009). Application of different levels of silver nanoparticles in food on the performance and some blood parameters of broiler chickens. World Appl. Sci. J. 7, 24-27.
Akbari M.R., Kemanshahi H. and Kelidari G.H.A. (2004). Investigating the effect of acetic acid in drinking water on performance, growth and microbial population of ileum in broiler chickens. J. Agric. Sci. Technol. Natur. Res. 3, 139-147.
Andi M.A., Mohsen H. and Farhad A. (2011). Effects of feed type with / without nanosil on cumulative performance, relative organ weight and some blood parameters of broilers. Glob. Vet. 7, 605-609.
Ashayerizadeh O., Dastar B., Shams Shargh M. and Khomeiry M. (2007). Evaluation of intestinal microbial population and the response of young broiler chickens to diets supplemented with Roxarsson, Avilamycin and Gold Formicin. J. Agric. Sci. Technol. Natur. Res. 43, 545-553.
Bailey M.T., Lubach G.R. and Coe C.L. (2004). Prenatal stress alters bacterial colonization of the gut in infant monkeys. J. Pediatr. Gastroenterol. Nutr. 38, 414-421.
Barbosa Fascina V., Roberto Sartori J., Gonzales E., Barros de Carvalho F., Mailinch Gonçalves Pereira de Souza I., do Valle Polycarpo G., Cristina Stradiotti A. and Cristina Pelícia V. (2012). Phytogenic additives and organic acids in broiler chicken diets. R. Bras. Zootec. 41, 2189-2197.
Burkholder K.M., Thompson K.L., Einstein M.E., Applegate T.J. and Patterson J.A. (2008). Influence of stressors on normal intestinal microbiota, intestinal morphology, and susceptibility to Salmonella enteritidis colonization in broilers. Poult. Sci. 87, 1734-1741.
Buzea C., Blandino I. and Robbie K. (2007). Nanomaterials and nanoparticles. Sources and toxicity. Biointerphases. 2, 17-172.
Byrd J.A., Hargis B.M., Caldwell D.J., Bailey R.H., Herron K.L., Mcreynolds J.L., Brewer R.L., Anderson R.C., Bischoff K.M., Callaway T.R. and Kubena L.F. (2001). Effect of lactic acid administration in the drinking water during pre-slaughter feed with drawal on Salmonella and Campylobacter. Poult. Sci. 80, 278-283.
Chen X. and Schluesener H.J. (2008). Nanosilver: A nanoproduct in medical application. Toxicol. Lett. 176, 1-12.
Daneshyar M., Pirmohamadi A. and Farhoomand P. (2015). Effect of Thymus vulgaris and Menthapulegium powders on performance, carcass characteristics and some blood parameters of broilers under heat stress condition. J. Iranian Vet. 4, 12-25.
Dhawale A. (2005). Better eggshell quality with a gut acidifier. J. Poult. Int. 44, 18-21.
Engberg R.M., Hedemann M.S., Lesser T.D. and Jensen B.B. (2000). Effect of zinc bacitracin and salinomycin on intestinal microflora and performance of broilers. Poult. Sci. 79, 1311-1319.
Fondevila M., Herrer R., Casallasa M.C., Abecia L. and Duchab J.J. (2008). Silver nanoparticles as a potential antimicrobial additive for weaned pigs. Anim. Feed Sci. Technol. 150, 259-269.
Garcia V., Catala-Gregori P., Hernandez F. Megias M.D. and Madrid J. (2007). Effect of formic acid and plant extracts on growth, nutrient digestibility, intestine mucosa morphology, and meat yield of broilers. J. Appl. Poult. Res. 16, 555-562.
Ghazalah A.A., Atta A.M., Elkoub K., Moustafa M.E.L. and Shata F.H. (2011). Effect of dietary supplementation of organic acids on perforamance, nutrients digestibility and health of broiler chicks. Poult. Sci. 10, 176-184.
Gornowicz E. and Dziadek K. (2002). The effect of acidifying preparations added to compound feeds on management conditions of broiler chickens. Ann. Anim. Sci. 1, 93-96.
Grudzien M. and Sawosz E. (2006). The influence of silver nanoparticles on chick embryo development and bursa fabricius morphology. Anim. Feed Sci. 15, 111-115.
Gunal M., Kaya G.O., Karahan N. and Sulak O. (2006). The effects of antibiotic growth promoter, probiotic or organic acid supplementation on performance, intestinal microflora and tissue of broilers. Int. J. Poult. Sci. 5, 149-155.
Hashemi S.R., Dastar B., Hassani S. and Jafari Ahangari Y. (2007). Growth performance, body temperature and blood proteins in broilers in response to betaine supplement and dietary protein level under heat stress. J. Agric. Sci. Natur. Res. 2, 138-147.
Hassanabadi A., Hajati H. and Bahreini L. (2012). The effects of nano-silver on performance, carcass characteristics, immune system and intestinal microflora of broiler chickens. Pp. 55-65 in Proc 3rd Int. Vet. Poult. Cong., Tehran, Iran.
Hume M.E., Corrier D.E., Ivie G.W. and Deloach J.R. (1993). Metabolism of propionic acid in broiler chicks. Poult. Sci. 72, 786-793.
Izat A.L., Tidwell N.M., Thomas R.A., Reiber M.A., Adams M.H., Colberg M. and Waldroup P.W. (1990). Effect of buffered propionic acid in diets on the performance of broiler chickens and on microflora of the intestine and carcass. Poult. Sci. 69, 818-826.
Lee D.N., Liu S.R., Chen Y.T., Wang R.C., Lin S.Y. and Weng C.F. (2007). Effects of diets supplemented with organic acids and nucleotides on growth, immune responses and digestive tract development in weaned pigs. J. Anim. Physiol. Anim. Nutr. 91, 508-518.
Lee S.J., Kim S.S., Suh O.S., Na J.C., Lee S.H. and Chung S.B. (1993). Effect of dietary antibiotics and probiotics on the performance of broiler. J. Agric. Sci. 35, 539-548.
Lesson S., Namkung H., Antongiovanni M. and Lee E.H. (2005). Effect of butyric acid on the performance and carcass yield of broiler chickens. Poult. Sci. 84, 1418-1422.
Levic J., Siniša M., Djuragi O.B. and Slavica S. (2008). Herbs and organic acids as an alternative for antibioticgrowth- promoters. J. Arch. Zootech. 11, 5-11.
Lin S.Y., Hung A.T.Y. and Lu J.J. (2011). Effects of supplement with different level of Bacillus coagulans as probiotic on growth performance and intestinal microflora populations of broiler chickens. J. Anim. Vete. Adv. 10, 111-114.
Lioyd J.R. (2003). Microbial reduction of Metals and Radionuclides. FEMS Microbial. Rev. 27, 412-425.
Morêki J.C. (2008). Feeding strategies in poultry in hot climate. Poult. Today. 601, 1-5.
Naghizadeh F. and KarimiTorshizi M.A. (2013). Evaluation of nanosilvers efficiency as an antibiotic substitute on performance and morphometric parameters of broiler chicks. Iranian J. Anim. Sci. 44, 255-262.
Naghizadeh F., KarimiTorshizi M.A. and Rahimi S. (2011). Comparison of nanosilver and in feed disinfectants on layer performance and intestinal microflora and yolk cholesterol. J. Anim. Prod. 13, 49-58.
Parks C.W., Grimes J.L., Ferket P.R. and Fairchild A.S. (2001). The effect of mannan oligosaccharides, mambermycins and virginiamycin on performance of large white male market turkeys. Poult. Sci. 80, 718-723.
Percival S.L., Bowler P.G. and Russell D. (2005). Bacterial resistance to silver in wound care. J. Hosp. Infect. 60, 1-7.
Pineda L., Chwalibog A., Sawosz E., Engberg C.R., Elnif J., Hotowy A., Sawosz F., Gao Y., Ali A. and Sepehri Moghaddam H. (2012). Effect of silver nanoparticles on growth performance, metabolism and microbial profile of broiler chickens. Arch. Anim. Nutr. 66, 416-429.
Sahin K., Sahin N., Onderci M., Yaralioglu S. and Küçük O. (2001). Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress. Vet. Med. Czech. 46, 140-144.
Saki A.A. and Salary J. (2013). Intravenous injection of silver nanoparticles and thyme and savory extracts on the 17th day of embryonic development and its effect on performance and blood parameters of broiler chicks on 14 and 21 days of breeding period. J. Anim. Sci. 101, 71-78.
SAS Institute. (2005). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sawosz E., Binek M., Grodzik M., Zielinska M., Sysa P. and Szmidt M. (2007). Influence of hydrocolloidal silver nanoparticles on gastrointestinal microflora and morphology of enterocytes of quails. Arch. Anim. Nutr. 61, 444-451.
Shameli K., Ahmad M.B., Zargar M., Yunus W.M.Z.W. and Ibrahim N.A. (2011). Fabrication of silver nanoparticles doped in the zeolite framework and antibacterial activity. Int. J. Nanomed. 6, 331-341.
Solhi Oskouyi A. (2016). Effect of silver nanoparticles coated on zeolite and organic acids on performance, the morphology of the intestine, gastrointestinal microbial population and carcass characteristics in broiler chickens under heat stress conditions. Ph D. Thesis. Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran.
Song J., Xiao K., Ke Y.L., Jiao L.F., Hu C.H., Diao Q.Y., Shi B. and Zou X.T. (2014). Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poult. Sci. 93, 581-588.
Vander Sluis A.A., Dekker M., Skrede G. and Jongen W.M. (2002). Activity and concentration of polyphenolic antioxidants in apple juice. 1. Effect of existing production methods. J. Agric. Food Chem. 50, 7211-7219.
Waldroup A. and Kanis W. (1995). Performance characteristics and microbiological aspects of broiler fed diets supplemented with organic acids. J. Food Prot. 58, 482-48.