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Open Access Article
1 - Evaluation of anti-bacterial property of modified Nano silver packaging with Photocatalyst TiO2 in sheep’s head and trotters
Sharifan, A., Beheshti Zadeh, R., Ahari, H.*, Anvar, A. .Head and trotters sheep is traditional food in Iran. This product has very microbial load then for first time determine to done it. The current study was designed to evaluate application of silver nanoparticles, as an antimicrobial agent. Effect of Nano silver pack MoreHead and trotters sheep is traditional food in Iran. This product has very microbial load then for first time determine to done it. The current study was designed to evaluate application of silver nanoparticles, as an antimicrobial agent. Effect of Nano silver packaging in reduce microbial load in head and trotters compare with Control sample in the Refrigerator for 4°C for 1,2,3,4 days. Minced Head and trotters sheep (100g of product has 15 g fat, 90 ml⁄gr Cholesterol). this product has bought at Shahrvand mall. Nano - silver coating based on titanium dioxide was used to reduce the microbial load of the product. some Nano-coating of silver on titanium dioxide at a concentration of ppm 1000, 2000, 3000, 3500 and 4000 used at this study. For measurement nanoparticles size used Field Emission Scanning Electron Microscope (fesem) and to know Chemical bond used. The results showed The concentrations of silver nanoparticles ppm 4000 ppm 3500 on Escherichia coli maintaining the relative amounts of byproducts in four days of testing on impact (p<0.05). Average of nanoparticle was. Manuscript profile -
Open Access Article
2 - The effect of nanosilver oral administration on body function, some blood and biochemical parameters of serum in chickens
M. Rahim, M. Talebi mehrdar,Silver has long been known for its antibacterial properties. In fact, silver nanoparticles, due to the release of silver ions, show such properties against aerobic and anaerobic bacteria and are widely used as additives in poultry diets. Regarding th MoreSilver has long been known for its antibacterial properties. In fact, silver nanoparticles, due to the release of silver ions, show such properties against aerobic and anaerobic bacteria and are widely used as additives in poultry diets. Regarding the antibacterial effects of this compound, research has shown contradictory results that do not indicate beneficial antibacterial effects. For this study, 240 one-day-old male chickens were divided into six groups with four replications. The experimental groups were: control diet (without additives), diet containing 10 ppm of the antibiotic avilamycin, diet containing 400 ml of silver nanoparticles per ton of feed, diet containing 800 ml of silver nanoparticles per ton of feed, diet containing 400 ml of silver nanoparticles per cubic meter of drinking water, diet containing 800 ml of silver nanoparticles per cubic meter of drinking water and to investigate the effect of silver nanoparticles on growth factor and body efficiency and some blood and serum parameters and microbial population of the device Digestive (ileum) of broiler chickens and comparison of antibacterial and growth stimulant effects with avilamycin antibiotic.The results showed a significant increase (P <0.05) in monocytes and chicken feed consumption and an increase in Clostridium population and a significant decrease in triglyceride concentration in the 800 ml group of silver particles in the diet and water (P <0.05), but its effect There was no significant effect on chicken weight gain and feed conversion ratio. Contrary to popular belief, silver nanoparticles are not suitable as a growth stimulant additive to control harmful bacteria in the poultry digestive tract and strengthen the immune system, increasing the feed conversion ratio of broilers. Manuscript profile -
Open Access Article
3 - The Effect of Nanofilm Coating Containing Ag on the Shelf Life of Mazafati Date
Seyyedeh Fatemeh Peyro Mousavi Amir Heidari Nasab Hassan HashemiPour Ali Asghar RajabalipourIntroduction: The aim of this study is to increase the shelf life and improve the quality of the product trade and export in Kerman Province by using coatings containing nanosilver particles and determining the optimal temperature for the storage of this product. Materi MoreIntroduction: The aim of this study is to increase the shelf life and improve the quality of the product trade and export in Kerman Province by using coatings containing nanosilver particles and determining the optimal temperature for the storage of this product. Materials and Methods: In this study, nanocomposite LDPE films containing nanosilver particles were prepared (3%, 5%), Dates from Aziz Abad, Bam were packed in nano-coated coverage and were stored at 50, 20, 8 and 4 UC. The samples were examined after 2, 7, 21 and 53 days. The experiments concerned with pH, TSS, acidity and reducing sugar were carried out according to the approved methods by the Research and Industry Standard Institute of Iran.Results: By increasing the acidity, the pH values decreased for all the samples examined including the control. The acidity for the samples containing 5% nanosilver particles was less than the samples containing 3% nanosilver particles and the control. The control sample showed lower concentration of the reducing sugar as compared to the samples containing 5 and 3% nanosilver particles while TSS values that are related to the ripening of the samples were different for all the samples examined.Conclusion: The samples packed with 5% nanosilver particles and kept for 53 days at 4UC showed better quality as compared to other samples. Manuscript profile -
Open Access Article
4 - اثر ضد باکتریایی نانوذرات نقره سنتز شده از عصاره گیاه گزنه Urtica diorica بر استافیلوکوکوس اورئوس و سودوموناس آئروژینوزا
امید زمانی زهرا کشتمند سیدمحمد مهدی حمدی -
Open Access Article
5 - سنتز بیولوژیکی نانوذره نقره از قارچ آسپرژیلوس فومیگاتوس و بررسی سمیت سلولی و خواص آنتی اکسیدانتی آن
فاطمه جلالیان لیلا پیشکار صاحبعلی منافی -
Open Access Article
6 - Investigating the morphophysiological characteristics and shelf life of Alstromeria spp. with the application of salicylic acid after harvesting
مهناز قنبری الهام دانائیThis research was designed in order to increase the quality and longevity of Alstroemeria cut flowers after harvesting. In this study, the puls treatment of salicylic acid at three levels of 50, 100 and 150 mg/liter and then the preservative solution of silver nanoparti MoreThis research was designed in order to increase the quality and longevity of Alstroemeria cut flowers after harvesting. In this study, the puls treatment of salicylic acid at three levels of 50, 100 and 150 mg/liter and then the preservative solution of silver nanoparticles 2 mg/liter with 3% sucrose was used. Distilled water and silver nanoparticles with sucrose were used as controls. The experiment was conducted as a factorial in the form of a completely randomized statistical design with 5 treatments and 3 replications (each treatment included 5 Alstroemeria cut flower branches). Sampling and measurement of desired traits were done on the 5th, 10th and 15th days of the experiment. The results showed that the highest relative fresh weight, solution absorption rate, percentage of cell membrane stability index and shelf life of Alstroemeria cut flowers were in salicylic acid 150+ silver nano particles+ sucrose Treatment. The highest amount of soluble solids, anthocyanin content and total chlorophyll was obtained in salicylic acid 100+ silver nanoparticles+ sucrose treatment. Therefore, concentrations of 100 and 150 mg/L of salicylic acid with nanoparticles 2 mg/L and sucrose 3% can be recommended to improve the morphophysiological characteristics and shelf life of Alstromeria spp. Manuscript profile -
Open Access Article
7 - One-Pot Synthesis of Some Heterocyclic Organic Compounds by Magnetic Nanoparticles and its Application in Electrosynthesis of Silver Nanoparticles
Seyed Hashem AkhlaghiHeterocyclic compounds are a subset of cyclic compounds inwhich the ring-forming atoms are composed of two or more different typesof atoms. Heterocyclic chemistry is an important part of organic chemistrythat studies the properties, production methods, and reactions ofh MoreHeterocyclic compounds are a subset of cyclic compounds inwhich the ring-forming atoms are composed of two or more different typesof atoms. Heterocyclic chemistry is an important part of organic chemistrythat studies the properties, production methods, and reactions ofheterocyclic compounds. Among the heterocyclic compounds, pirans areone of the most important and valuable categories of heterocycles that havemany applications in medicine and therapy. In this project, we intend tosynthesize three of the Pirans derivatives under temperature and refluxconditions. The purpose of synthesizing these derivatives is theimmobilization of Ag nanoparticle, with uniform distribution, on thesurface of glassy carbon electrode such that the electrocatalytic current ofAg nanoparticle be constant. Manuscript profile -
Open Access Article
8 - Effects Of Intraperitoneal Administration Of Nano-Silver on Rat Hippocampal Cells
Hamid Nasrollahzade Parvin Khodarahmi Mitra Heidari NasrabadiInroduction and Objective: Silver nanoparticles are very promising engineered which play an important role in the world biomedical, healthcare and in general nanotechnology applications. In this study, the necrotic effects of short and long term administrations of nan MoreInroduction and Objective: Silver nanoparticles are very promising engineered which play an important role in the world biomedical, healthcare and in general nanotechnology applications. In this study, the necrotic effects of short and long term administrations of nanosilver on rat hippocampus, which is involved in memory and learning, was investigated.Materials and Methods : 56 male Wistar rats were divided into two groups of short term and long term injections which were treated for 5 and 10 consecutive days, respectively. Each group was divided into four subgroups of control, 100, 200 and 400 ppm nanosilver administration. The control subgroup received saline and the treatment subgroups received intraperitoneal injections of silver nanoparticles at doses of 100, 200 and 400ppm. Ten days after the last injection, the hippocampal tissue sections were prepared and the extent of necrosis was evaluated stained with Nissl.Results:In short term (5-day injection) group, the percentage of necrotic cells between control and 100(pConclusion:Silver nanoparticles induce necrosis in rat hippocampal cells. For the administration regime considered in this study, this apoptotic effect increases at higher doses but is independent of the duration of exposure Manuscript profile -
Open Access Article
9 - The Antibiotic Resistance Pattern of Methicillin Resistant Staphylococcus aureus and Antibacterial Effect of Silver and Copper Nanoparticles on that In vitro and Animal model
Saba Hashemi Rasoul ShokriBackground & aim: Considering the high resistance to drug in methicillin-resistant Staphylococcus aureus, this study was performed to investigate the effects of silver and copper nanoparticles and their combined effect on the methicillin-resistant Staphylococcus aur MoreBackground & aim: Considering the high resistance to drug in methicillin-resistant Staphylococcus aureus, this study was performed to investigate the effects of silver and copper nanoparticles and their combined effect on the methicillin-resistant Staphylococcus aureus in vitro and animal models.Materials & Methods: To determine the most effective antibiotics, disk diffusion method was performed. The microdilution method is used for determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of copper and silver nanoparticles and their combination . Then, to examine the effect of nanoparticles and the antibiotic, experiments were performed in the animal models. For this purpose, after intraperitoneal inoculation of bacterial suspension, MBC concertrations obtained from microdilution method were injected to the mice and after the cultivation of spleen, colony count was performed.Results: The most effective antibiotic on bacteria was vancomycin. The MIC of silver and copper nanoparticles, vancomycin, silver and copper nanoparticles, silver nanoparticles-vancomycin and copper nanoparticles- vancomycin were 1250 ppm, 2500 ppm, 78/12, 625 ppm, 35 ppm and 250 ppm respectively. In animal model, the lowest number of colonies is related to silver nanoparticles and then silver nanoparticles-vancomycin.Conclusion: The results show that antimicrobial effect of silver nanoparticles is more than vancomycin and copper nanoparticles. Also the combination of silver and copper nanoparticles increases their antimicrobial strength and the combination of silver and vancomycin after complementary experiments, can be a option for the treatment of infections caused by these bacteria. Manuscript profile -
Open Access Article
10 - Investigating the effects of silver nano particles on fungi flora of seeds of Chamran wheat cultivar
Mohsen Ghamari Masoud Latifian Mansour Farbod Nazanin Amir BakhtiarThis study was carried out in order to investigate the possibility of controlling seed-born disease of wheat Chamran cultivar by nano silver seed coating and indicate relevant concentration of nano silver on fungal seed-born of wheat at Khouzestan-Rami MoreThis study was carried out in order to investigate the possibility of controlling seed-born disease of wheat Chamran cultivar by nano silver seed coating and indicate relevant concentration of nano silver on fungal seed-born of wheat at Khouzestan-Ramin Agricultural and Natural Resources University. Experiment was conducted in the form of completely randomized design with four replications and six treatments. Treatments included one level of disinfection by 2.5% sodium hypochlorite and five levels of seed coating by different concentration of silver nano particle (S1 = 1.30, S2 = 4, S3 = 6.70, S4 = 9.3 and S5 = 2666 mg of silver nitrate solution). Results showed that there was a significant difference among disinfection levels (P≤0.01) for disease index. In comparison with sodium hypochlorite, the Disease Index was declined about 50% by S1 treatment. The most favorable effect on decreasing Disease Index was observed with S5 treatment where it dropped up to 75%. According to results the mean of mortal concentration of nano silver on dominant species (Aspergillus sp.,fusarium graminiarum, Penicilium sp ., Torula sp., Alternaria alternaria) was calculated using Fini method in order to approach the finest concentration. The maximum LC50 was obtained for Torula sp. 1875.6 ml/lit and the minimum 0.02 ml/lit was for Aspergillus sp. and Alternaria alternaria. Regarding to this point that fungal seed-borne are active on seed bed in the form of a band, for approach to efficient disinfection it is essential to use the maximum needed concentration. Manuscript profile -
Open Access Article
11 - The effect of synthetic silver nanoparticle from hydroalcoholic extract Digitalis nervosa on CDH1, Bax , Bcl2 and P53 gene expressions in cervical cancer cell line)Hela(
nazila ahmadzadeh nastran asghari moghaddam zahra keshtmandNowadays, metal nanoparticles, especially silver, due to their special physical properties and anti-cancer effects have been considered.One of the methods of synthesis of these nanoparticles is the use of plant extracts. The aim of this study was to investigate the anti MoreNowadays, metal nanoparticles, especially silver, due to their special physical properties and anti-cancer effects have been considered.One of the methods of synthesis of these nanoparticles is the use of plant extracts. The aim of this study was to investigate the anti-cancer effects of silver nanoparticles synthesized of the hydroalcoholic extract of Digitalis nervosa on the cancer cell line (Hela).In this experimental study,the synthesis of silver nanoparticles was performed by adding the hydroalcoholic extract of Digitalis nervosa to the silver nitrate solution. Physical and chemical properties of synthesized nanoparticles were determined by UV-vis, TEM, SEM, EDX and XRD methods.Viability of Hela cancer cells by MTT method with different concentrations of synthesized silver nanoparticles(3.125 to 100 mg/ml) and the expression of Bax, Bcl2, P53 and CDH1 genes were evaluated using real time PCR technique in 24 hours.Data were analyzed using GraphPad software and one-way ANOVA test.P<0.05 was considered statistically significant.The results showed that the results of cytotoxicity at different concentrations of silver nanoparticles showed that IC50 was16.66 μg /ml, respectively. Also, the expression of Bax, CDH1 and P53 apoptotic genes in concentration-(16.66μg/ml) increased and Bcl2 gene expression decreased compared to the reference gene, which showed the effects of cytotoxicity and changes in nanoparticle concentration. Silver nanoparticles synthesized from the extract of the Digitalis nervosa plant have a cytotoxic effect and possibly induce apoptosis on Hela cancer cells. Manuscript profile -
Open Access Article
12 - The Effect of Silver Nanoparticles Produced from Lactobacillus plantarum Supernatant on Pathogenic Bacteria
Ali Sardarian Esmaeil Ataye Salehi Akram Arianfar Reza Safari -
Open Access Article
13 - Investigation of Antioxidant and Antimicrobial Properties of Nanocomposite Films Containing Synthesis of Silver Nanoparticles with Mentha extract
Bahareh Haji Rostamloo Raheleh Zhiani Fatemeh Amarloo -
Open Access Article
14 - پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی
ا. عباسی س.ر. هاشمی س. حسنی م. ابراهیمیهدف از این آزمایش ارزیابی پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی میباشد. به همین منظور در این آزمایش از 375 قطعه جوجه گوشتی یکروزه نژاد کاب (500 Cobb) استفاده شد. که در 5 تیمار Moreهدف از این آزمایش ارزیابی پاسخ جمعیت میکروبی دستگاه گوارش و عملکرد جوجههای گوشتی تغذیه شده با اسیدهای آلی و نانو نقره پوشش داده شده بر زئولیت تحت تنش گرمایی میباشد. به همین منظور در این آزمایش از 375 قطعه جوجه گوشتی یکروزه نژاد کاب (500 Cobb) استفاده شد. که در 5 تیمار و 5 تکرار و 15 قطعه جوجه در هر تکرار در قالب یک طرح کاملاً تصادفی تقسیم شدند. پنج تیمار غذایی شامل: 1) جیره شاهد، 2) جیره شاهد حاوی 1 درصد زئولیت، 3) جیره شاهد حاوی 1 درصد زئولیت پوشش داده شده با 0.5 درصد نانو نقره، 4) جیره شاهد حاوی 1 گرم بر کیلوگرم اسید آلی و 5) جیره شاهد حاوی 1 درصد زئولیت پوشش داده شده با 0.5 درصد نانو نقره و 1 گرم بر کیلوگرم اسید آلی بود. خوراک مصرفی و وزن بدن برای هر تیمار در کل دوره آزمایش ثبت و ضریب تبدیل خوراک محاسبه گردید. به منظور شمارش جمعیت میکروبی دستگاه گوارش دو قطعه جوجه از هر واحد آزمایشی در روزهای 21 و 42 پرورش کشتار شدند. نتایج آزمایش نشان داد که جیره غذایی حاوی نانو نقره به همراه اسید آلی باعث کاهش وزن بدن جوجههای گوشتی در مقایسه با تیمار شاهد و زئولیت در فاصله سن 42-21 روزگی شد (P<0.05). همچنین در کل دوره پرورش ضریب تبدیل غذایی در گروه نانو نقره پوشش داده شده بر زئولیت به طور معنی ­داری نسبت به گروه شاهد و زئولیت بالاتر بود (P<0.05). با این حال تیمارهای آزمایشی هیچگونه اختلاف معنیداری بر جمعیت میکروبی دستگاه گوارش نداشتند (0.05<P). به طور خلاصه نتایج حاضر نشان داد اگرچه نانو ذرات نقره و اسیدهای آلی اثر خاصی بر پارامترهای عملکرد و افزایش شمار باکتریهای مفید (اسید لاکتیک) دستگاه گوارش در حالت تنش گرمایی نداشتند اما موجب کاهش آنها نگشته­اند. بنابراین میتوان از آنها به عنوان افزودنی در جیره غذایی جوجههای گوشتی استفاده نمود. Manuscript profile -
Open Access Article
15 - اثر تیمار کوتاه مدت با نانو ذرات نقره و محلول گلجایی ساکارز روی پیری گل و برگ شاخه بریده رز
خدیجه آل کثیر رحیم نقش بند حسنی علیرضا مطلبی آذراین آزمایش برای ارزیابی اثر تیمار کوتاه مدت نانو ذرات نقره در غلظت­ های صفر، 4، 25، 75 و 125 میلی­ گرم در لیتر روی گل شاخه بریدنی رز رقم ʼهای اند مجیکʻ بدنبال نگهداری در محلول ساکارز با غلظت­ های صفر، 2 و 3 درصد از نظر عمر گلجایی و برخی از صفات فیزیولوژیکی پ Moreاین آزمایش برای ارزیابی اثر تیمار کوتاه مدت نانو ذرات نقره در غلظت­ های صفر، 4، 25، 75 و 125 میلی­ گرم در لیتر روی گل شاخه بریدنی رز رقم ʼهای اند مجیکʻ بدنبال نگهداری در محلول ساکارز با غلظت­ های صفر، 2 و 3 درصد از نظر عمر گلجایی و برخی از صفات فیزیولوژیکی پس از برداشت آن انجام شد. این تحقیق به شکل آزمایش فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار برای هر تیمار اجرا شد. آزمایش تحت شرایط دمای 2 ± 23 درجه سانتی­گراد، رطوبت نسبی 5±60 درصد و با شدت نوری محیط 12 میکرو مول در مترمربع درثانیه به مدت 12 ساعت توسط لامپ­ های فلورسنت انجام شد. نتایج نشان داد که تیمار 125 میلی­ گرم در لیتر نانو ذرات نقره همرا با ساکارز دو درصد و بدون ساکارز بیشترین تاثیر را در کنترل باکتری­ های انتهای ساقه را داشت. تیمار کوتاه مدت نانو ذرات نقره در کلیه غلظت­ ها سبب بهبود جذب محلول، حفظ رطوبت نسبی شاخه، بهبود باز شدن گل و شاخص فلورسانس کلروفیل برگ (Fv/Fm) در مقایسه با تیمار شاهد گردید. بیشترین میزان باز شدگی گل با درشت­ترین اندازه گل در تیمار 75 میلی­ گرم در لیتر نانوذرات همراه با ساکارز 2 درصد در روز نهم عمر گلجایی مشاهده گردید. کمترین میزان عمر گلجایی در تیمارهای آب مقطر به تنهایی و همراه با ساکارز بود. بیشترین میزان عمر گلجایی در تیمارهای 75 و 125 میلی­ گرم در لیتر نانو ذرات نقره بدون ساکارز و با ساکارز در مقایسه با سایر تیمارها مشاهده گردید. Manuscript profile -
Open Access Article
16 - Preparation of Double-layer Nanofibers Base on Hydrogel, Silver nanoparticles, and Fatty Acid using electrospinning technique
ensieh ghasemian lemraski elaheh khajehali sahar yariToday, electrospinning method is widely used to produce nanofibers based on polymers. Poly (vinyl-alcohol) and chitosan are among the most widely used polymers in biochemistry and medicine, especially in wound healing and tissue engineering. In this project, an attempt MoreToday, electrospinning method is widely used to produce nanofibers based on polymers. Poly (vinyl-alcohol) and chitosan are among the most widely used polymers in biochemistry and medicine, especially in wound healing and tissue engineering. In this project, an attempt has been made to make polymeric materials and silver nanoparticles, nanofibers with protection and antimicrobial capabilities. Electrospinning method for making bilayer nanofibers, top layer containing poly (vinyl alcohol) and chitosan containing silver nanoparticles or silver ions as antimicrobial layer, and bottom layer containing polyvinylpyrrolidone and fatty acid as layer Moisturizes the skin of injured skin. Morphology and structure of nanofibers produced using various methods such as XRD X-ray spectroscopy, FTIR infrared Fourier transform spectroscopy, thermal weighing method or TGA, X-ray energy diffraction spectroscopy (EDAX-EDS), electron microscopy method (SEM), and finally the transmission electron microscopy (TEM) method was examined. The results of the instrumental analysis indicate the production of polymer fibers with a diameter in the range of 200-500 nanometers, which indicates the successful production of nanofibers, and also structural analyzes confirm the presence of silver nanoparticles and silver ions. The results of microbial tests show that nanofibers containing silver ions have a greater antimicrobial effect than silver nanoparticles. Manuscript profile -
Open Access Article
17 - Biosynthesis and characterization of silver nanoparticles using aqueous extract of walnut (Juglans regia L.) leaf
masoud bayat reza moradi ali bodaghiGreen synthesis of nanoparticles is an environmentally friendly method, in which water solvent are used. In this study, the biosynthesis of silver nanoparticles (Ag-NPs) was performed by using walnut (Juglans regia L.) leaf extract. Walnut leaf contains a variety of che MoreGreen synthesis of nanoparticles is an environmentally friendly method, in which water solvent are used. In this study, the biosynthesis of silver nanoparticles (Ag-NPs) was performed by using walnut (Juglans regia L.) leaf extract. Walnut leaf contains a variety of chemicals, including tannins, flavonoids, vitamins, plant acids and naphthoquinone. The extract of walnut (Juglans regia L.) leaf as the reducing agent is used for the synthesis of silver nanoparticles. The silver nanoparticles have been characterized by scanning electron microscopy (SEM), Energy dispersive X-ray (EDAX), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and UV-Visible spectroscopy. UV-Vis spectrum exhibit an absorption band at around 400-450 nm suggestion the formation of silver nanoparticles. The observed peak at about 425 nm is related to the surface plasmon resonance of silver nanoparticles. The effects of operational parameters in the synthesis of silver nanoparticles such as silver nitrate salt concentration, extract volume, temperature and time was studied. The results showed that silver nitrate concentration 6 mM, extract volume 200 µlit, temperature 90°C and time 40 min was the optimum amounts. Manuscript profile -
Open Access Article
18 - Antibacterial effect of silver nanoparticles synthesized from the red algae Gracilaria gracilis
somayeh Ataei- e jaliseh maryam zamani kochesfehni mohammad hossein zamani kochesfehaniSilver nanoparticles (Ag-np) have high penetration and antimicrobial effect due to their high surface-to-volume ratio. The aim of this study was to biosynthesize silver nanoparticles with red algae extract, Gracilaria gracilis, and to investigate their MoreSilver nanoparticles (Ag-np) have high penetration and antimicrobial effect due to their high surface-to-volume ratio. The aim of this study was to biosynthesize silver nanoparticles with red algae extract, Gracilaria gracilis, and to investigate their antibacterial activity against a number of standard and drug-resistant pathogenic bacteria. First, Ag-np were synthesized. To confirm the structure and size of Ag-np, was used X-Ray diffraction spectroscopy, FE-SEM electron microscopy, and X-ray energy dispersive spectroscopy (EDS). The antimicrobial effects of algae extract on bacteria were determined by sequential dilution method. The size of nanoparticles under electron microscopy was between 12 and 46 nm. The nanoparticles were able to inhibit most of standard and antibiotic resistant bacteria, Ag-np at a concentration of 29 μg /ml, on the standard bacteria: S. typhimorium, E. coli, K. pneumonia and the clinically resistant bacteria, E. coli and K. pneumonia, they had the most inhibitory effect. In contrast, standard and clinically resistant isolates of S. aureus and standard strain S. pneumonia were resistant to Ag-np. The results of this research showed that the G. gracilis red algae as a bio-source that can be useful for green synthesis of silver nanoparticles at very low cost applications, these nanoparticles can be used as candidates for drug composition. Manuscript profile -
Open Access Article
19 - Study of ability Fusarium oxysporum fungi in extracellular biosynthesis of silver nanoparticle in vitro
Golshid Sajjadi Abolfath Shojaiee Mohammad Reza Fazeli Javid Amini Hossain JamalifarBackground and Objective: Development of methods without using plotant and poisonous material and minimum wastes to produce under control nano structures is of nanotechnologist concerns. In this way biologists, by previous knowledge, nano-scale minerals controlled makin MoreBackground and Objective: Development of methods without using plotant and poisonous material and minimum wastes to produce under control nano structures is of nanotechnologist concerns. In this way biologists, by previous knowledge, nano-scale minerals controlled making by microorganism, seeking organism capable making non-organic nanoparticles. The aim of this study is extracellular production of silver nanoparticles with maximum dimention of 20 nm by Fusarium oxysporum fungi. Materials and methods: After studies to optimize growth condition, Fusarium oxysporum biomass in a medium containing yeast and malt extract is reproduced. After silver nanoparticles production in silver nitrate solution, this nanoparticles are studies by UV-Visible Spectrophotometry and Transmission Electron Microscopy (TEM) methods. Results: Studies showed that when Fusarium oxysporum biomass put in 10-3 M of silver ions, can produce silver nanoparticles in the form of extracellular. Conclusion: Because of physical and chemical particular properties of silver nanoparticles with maximum diameter of 20 nm of Fusarium oxysporum fungi, its industrial production and applied evaluation is offered. Manuscript profile -
Open Access Article
20 - The Inhibitory Effect of Doxycycline- Silver Nanoparticle Conjugate on Brucella melitensis
Mohsen Ajalli Mojtaba Salouti Hamed Alizadeh Zahra Heydari Hossein Hamzehei Aram AlizadehBackground and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver MoreBackground and Objective: Brucellosis is a zoonotic disease caused by Brucella bacteria. According to recent studies, antimicrobial activity of silver nanoparticle was approved. The aim of this study was to investigate the antimicrobial activity of Doxycycline - silver nanoparticles conjugate against Brucella meltensis 16M. Material and Methods: After preparing the doxycycline-silver nanoparticle conjugate, its antimicrobial activity against Brucella meltensis 16M was determined by Well Diffusion Agar method in Muller Hintone Agar media. Also, Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Doxycycline-silver nanoparticle conjugate was determined by Macrodilution method in Muller Hintone Broth media. Finally, antibacterial effect of the nanoparticle was assayed in animal model. Results: The results showed that Doxycycline-silver nanoparticle conjugate has antimicrobial activity against Brucella melitensis 16M in laboratory condition. In mouse model, the conjugate of Doxycycline-silver nanoparticle could decrease effectively the Brucella melitensis load in liver. Conclusion: This study demonstrated that Doxycycline-silver nanoparticle conjugate has synergistic effect on Brucella melitensis 16M and can be useful in treatment of brucellosis. Manuscript profile -
Open Access Article
21 - بررسی کاربرد نانو ذرات نقره بیوسنتز شده با استفاده از عصاره گیاه دارویی زنیان (Trachyspermum ammi) در کنترل پوسیدگی باکتریایی پیاز
زینب فتوحیانامروزه استفاده از نانوذرات فلزی به عنوان جایگزین مناسب برای سموم شیمیایی خطر آنها را کاهش میدهد. لذا هدف از مطالعه حاضر بیوسنتز نانوذرات نقره با استفاده از عصاره آبی دانه زنیان بومی منطقه و بررسی اثر آن بر علیه باکتری پوسیدگی پیاز میباشد. در این پژوهش ابتدا جداسازی و Moreامروزه استفاده از نانوذرات فلزی به عنوان جایگزین مناسب برای سموم شیمیایی خطر آنها را کاهش میدهد. لذا هدف از مطالعه حاضر بیوسنتز نانوذرات نقره با استفاده از عصاره آبی دانه زنیان بومی منطقه و بررسی اثر آن بر علیه باکتری پوسیدگی پیاز میباشد. در این پژوهش ابتدا جداسازی و شناسایی باکتری عامل بیماری از مناطق مختلف شهرستان جیرفت انجام گردید. آنگاه بیوسنتز نانوذرات نقره توسط عصاره آبی دانه زنیان و اثر آنها علیه پکتوباکتریوم در شرایط آزمایشگاه و انبار بررسی شد. مجموعاً 12 ایزوله باکتری جداسازی و ایزوله N3از منطقه عنبرآباد دارای بیشترین شدت بیماریزایی بود. بیوسنتز نانوذرات نقره توسط عصاره با مشاهده رنگ خرمایی بعد از گذشت 15 دقیقه در شرایط تاریکی تایید گردید و بررسی طیف سنج ماورابنفش مشخص نمود که پلاسمون رزونانس سطحی در طول موج 420 نانومتر است. میزان بازدارندگی از رشد نانوذرات نقره بیوسنتز شده و سم مانکوزب هر کدام به تنهایی و ترکیب آنها علیه پکتوباکتریوم در شرایط آزمایشگاه به ترتیب 62.45، 100 و88.67 درصد بود. همچنین نانوذرات نقره و سم مانکوزب هر کدام به تنهایی طی سه ماه و تلفیق آنها با هم طی دو ماه به میزان100 درصد باعث ماندگاری پیاز در انبار شدند. اما تلفیق نانوذرات نقره و سم مانکوزب به همراه پکتوباکتریوم در ماه اول و دوم، 100 درصد و در ماه سوم، 85 درصد علیه بیماری پوسیدگی پیاز بازدارندگی داشتند. بنابراین نتایج نشان داد که نانوذرات نقره سنتز شده بر کنترل باکتری بیمارگر در شرایط آزمایشگاه و انبار موثر بودند و میتوانند جایگزین سموم کشاورزی شوند. Manuscript profile -
Open Access Article
22 - The Effect of Silver Nanoparticles on the Viability of Lung Fibroblast Cell Line (MRC-5)
Yasaman Dastgir Zahra Keshtmand Katayoun BorhaniSilver nanoparticles (AgNPs) with very strong antimicrobial and anti-proliferative properties are widely used in medical sciences. However, little is known about their effect on healthy cells, and there were the reports of their toxicity. This study aimed to investigate MoreSilver nanoparticles (AgNPs) with very strong antimicrobial and anti-proliferative properties are widely used in medical sciences. However, little is known about their effect on healthy cells, and there were the reports of their toxicity. This study aimed to investigate the effect of silver nanoparticles on the viability rate of the MRC-5 cell line. In this experimental study, during 24, 48, and 72 hours, different concentrations of silver nanoparticles (1.56, 3.125, 6.5, 12.5, 25, 50, and 100 μg/ml) per category MRC-5 cells were examined by MTT assay. Data were analysed using SPSS software, one-way ANOVA, Tukey test, and a significant level of p < /em> < 0.05. The treatment of normal MRC-5 cells with different concentrations of silver nanoparticles after 24, 48, and 72 hours by MTT method showed a significant reduction in the viability of cells at concentrations of 25, 50, and 100 μg/ml (p < /em> < 0.05). The results of this study showed that silver nanoparticles have a dose-dependent inhibitory effect on normal MRC-5 cells. Manuscript profile -
Open Access Article
23 - Phytosynthesis of silver nanoparticles using Otostegia persica
halime gholami ebrahim molashahi fateme sargezy alireza aeinaliIn this study we researched about the synthesis of silver nanoparticle by the aqueous extract of Otostegia persica. In the first the results of HPLC was showed extracts of Otostegia persica contains poly phenols compound such as sinapic acid, caffeic acid, trans-feralic MoreIn this study we researched about the synthesis of silver nanoparticle by the aqueous extract of Otostegia persica. In the first the results of HPLC was showed extracts of Otostegia persica contains poly phenols compound such as sinapic acid, caffeic acid, trans-feralic, gallic acid, p-coumaric acid and vanillin that sinapic acid, caffeic acid, trans-feralic are significant compound. Therefore, in this research, we have attempted to synthesize nanoparticles by using the aqueous extract of the Otostegia persica, which contains compounds such as sinapic acid, caffeic acid, trans-feralic acid, gallic acid, p-coumaric acid and vanillin. In this study, it was found that the aqueous extract of Otostegia persica is able to synthesis silver nanoparticles. In this research, after the reaction of solutions with each other, the first step was the color change from green to brown. Based on previous research, the reaction mixture changes color by adding different concentrations of metal ions. These color changes are caused by the stimulation of surface plasmon vibrations in silver nanoparticles, so the change in the color of the reactive mixture indicates the synthesis of silver nanoparticles. The aqueous extract of Otostegia persica was used as a stabilizer or capping agent and reducing agent to reduce Ag+ to metallic silver. The phenolic compounds in the Otostegia persica extract have the ability to react with silver ions in silver nitrate solution and exchange electrons and reduce silver ion (Ag+) to neutral silver (Ag0) in nano dimensions. Then we tried to optimize the research and the various components were optimization such as the time, pH, the concentration of extract and silver nitrate. Finally, the volume of the extract was analyzed and by using UV device, we proved the formation of silver nanoparticles and was observed a peak in the area of 734 nm. The results were showed that in the concentration of 1 mM from silver nitrate and the temperature of 22 C and pH=8 are the optimal conditions for the formation of silver nanoparticles. Then were sampled from the silver nanoparticles, the average size of silver particles and the structure of nanoparticles were determined by using TEM duvice and the size of nanoparticle it was found 34 nm and its shape was observed as a sphere. Then we using FTIR and XRD for proved the formation of silver nanoparticles and we proved the existence of silver nanoparticles. Therefore, in this study, it was found that using polyphenolic compounds in the aqueous extract of Otostegia persica as a natural, harmless, inexpensive and with antioxidant and reducing properties can be used for the synthesis of metal nanoparticles, including silver nanoparticles that these silver nanoparticles are very important in the medical and pharmaceutical industries. The general conclusion from this research is the high value and importance of medicinal plants in the green synthesis of nanoparticle, which is a suitable alternative instead of using harmful chemicals, including the Otostegia persica, which has the ability to be planted and harvested in areas has different varieties of Iran and the plant is rich in secondary compounds, including polyphenolic compounds with high reductive and antioxidant properties, which is a suitable option for synthesis nanoparticles with appropriate shape and size. Manuscript profile
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