Effects Of Intraperitoneal Administration Of Nano-Silver on Rat Hippocampal Cells

Subject Areas :

Hamid Nasrollahzade ¹ , Parvin Khodarahmi ² , Mitra Heidari Nasrabadi ³

1 - کارشناسی ارشد زیست شناسی سلولی-تکوینی گروه زیست شناسی واحد پرند، دانشگاه آزاد اسلامی، پرند. ایران.
2 - استادیار گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند. ایران
3 - دانشیار گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی، پرند. ایران.

Received: 2015-05-20 Accepted : 2018-08-04 Published : 2014-04-21

Keywords: Necrosis, Nissl Staining, Hippocampus, nano silver,

Abstract :

Inroduction and Objective: Silver nanoparticles   are very promising engineered which play an important role in the world biomedical, healthcare and in general nanotechnology applications. In this study, the necrotic effects of short and long term administrations of nanosilver on rat hippocampus, which is involved in memory and learning, was investigated.Materials and Methods : 56 male Wistar rats were divided into two groups of short term and long term injections which were treated for 5 and 10 consecutive days, respectively. Each group was divided into four subgroups of control, 100, 200 and 400 ppm nanosilver administration. The control subgroup received saline and the treatment subgroups received intraperitoneal injections of silver nanoparticles at doses of 100, 200 and 400ppm. Ten days after the last injection, the hippocampal tissue sections were prepared and the extent of necrosis was evaluated stained with Nissl.Results:In short term (5-day injection) group, the percentage of necrotic cells between control and 100(pConclusion:Silver nanoparticles induce necrosis in rat hippocampal cells. For the administration regime considered in this study, this apoptotic effect increases at higher doses but is independent of the duration of exposure