Abstract :
In this study, an effective electrochemical sensor for the rapid measurement of L-cysteine based on glassy carbon electrode modified with polyindole/silver nanoparticles nanocomposite is presented. Polyindole nanofibers were synthesized by cyclic voltammetry method at the glassy carbon electrode surface, and then silver nanoparticles were deposited on these nanofibers using a constant potential method. Surface morphology and characterization of the modified electrodes were confirmed by field emission scanning microscopy (FE-SEM), X-ray diffraction (XRD), Raman, and FT-IR spectroscopies. The electrochemical investigation showed that the polyindole/silver nanoparticles had very good efficiency with respect to the electrocatalytic oxidation of L-cysteine in phosphate buffer solution (pH = 7.0). The response of the glassy carbon electrode/polyindole/silver nanoparticles to L-cysteine was linear in the concentration range of 0.01-10 mM. The detection limit was obtained at signal/noise=3, 5.7 μM. In addition, the sensor showed good stability and repeatability. The application of the proposed sensor for the analysis of L-cysteine in human serum was successful.
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