The effect of silver nanoparticles on the expression of exotoxin A and S genes in Pseudomonas aeruginosa
Subject Areas : microbiology
Muhammad Hemati
1
,
Zahra Keshtmand
2
,
Katayoun Borhani
3
1 - Masters Student, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2 - Assistant Professor, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3 - Assistant Professor, Department of Environment and Food industry Engineering, Central Tehran Branch,
Islamic Azad University, Tehran, Iran
Keywords: silver nanoparticles, Pseudomonas aeruginosa, exotoxin A, Exotoxin S,
Abstract :
Background: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been widely considered in the field of medicine and medicine, especially against microbes. The aim of the present study was to investigate the effect of silver nanoparticles (AgNPs) on the expression of Pseudomonas aeruginosa exotoxin A and S genes. Methods: In this experimental study, the minimum growth inhibition concentration of silver nanoparticles was determined by microdilution method. First, the lowest concentration of bacterial growth inhibitor is determined with silver nanoparticles. Then, at a concentration lower than the lowest growth rate, the expression of Pseudomonas aeruginosa exotoxin A and S genes was examined by Real time PCR. Data were analyzed using one-way ANOVA, Tukey test and P value less than 0.05. Findings: Silver nanoparticles with a concentration of 62.5 μg / ml had growth inhibitory effects on Pseudomonas aeruginosa. Also, at a concentration of 31.25, the expression of Pseudomonas aeruginosa exotoxin A and S genes was significantly reduced (p < 0.05). Conclusion: The results of this study show that lower concentrations (31.25 μg/ml) than growth inhibition concentrations (62.5μg/ml) can reduce the expression of Pseudomonas aeruginosa exotoxin A and S genes.
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Motaghi B & Mojafipour S. Outer Membrance Protein D Gene in clinical Isolates of Pseudomonas Aeruginosa and its Role in Antibiotic Resistance. Journal of Fasa University of medical Sciences. 2016; 5(4): 501-507.
Tang Y & et al. Genotyping of Pseudomonas aeruginosa Type III Secretion System Using Magnet ic EnrichmentMultiplex Polymerase Chain Reaction and Chemiluminescence. Journal of Biomedical Nanotechnology.2016; 12(4): 762-769.
Mokari M, Owlia P, Marashi SMA, Saderi H & Dehghan Zadeh Z. The effect of supernatant of Saccharomyces cerevisiae yeast on preventing the growth of Pseudomonas aeruginosa bacteria and its effect on exotoxin S gene expression in Pseudomonas aeruginosa bacteria by Real-Time PCR methodd. Daneshvar Medicine: Basic and Clinical Research Jouranl. 2018; 25(6): 67-74.
BouillotS,AttréeI & HuberP. Pharmacological Activation of Rap1 Antagonizes the Endothelial Barrier Disruption Induced by Exotoxins ExoS and ExoT of Pseudomonas aeruginosa. Infect Immun. 2015; 83(5): 1820-1829.
Pavlovskis OR, Iglewski BH & Pollack M. Mechanism of action of Pseudomonas aeruginosa exotoxin A in experimental mouse infections: adenosine diphosphate ribosylation of elongation factor 2. Infection and Immunity. 1978; 19(1): 29-33.
Donelli G & Vuotto C. Biofilm based infections in long term care facilities. Future Microbiology. 2014; 9: 175-188.
Tran QH, Nguyen VQ & Le A. Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Advances in Natural Sciences:Nanoscience and Nanotechnology. 2013; 4(3).
Mortazavi H, Nakhaei Moghaddam M & Abadi NS. Study of the Effect of Silver Nanoparticles on Biofilms Formation by Staphylococcus epidermidis. Journal of Rafsanjan University of Medical Sciences. 2015; 14(2): 125-136.
Björndahl MR, Cao LJ, Nissen S, Clasper LA, Johnson Y, Xue Z & et al. Lnsulin-like growth factors 1 and 2 induce lymphangiogenesis in vivo. Proceedings of the National Academy of Sciences. 2005; 102(43): 15593-15598.
Heinlaan M, Lvask A, Blinov L, Dubourgier HC & Kahur A. Toxicity of nanosized and bulk Zno, Cuo and Tio 2 to bacteria vibrio Fischer and crustaceans Daphnia magna and Thamnocephalus platyurus. Chemosphere. 2008; 71(7): 1308-1316.
Fernando S, Gunasekara T & Holton J. Antimicrobial Nanoparticles: Applications and mechanisms of action. Sri Lankan Journal of Infectious Diseases. 2018; 8(1): 2-11
Zargar M & Mohamadeibandarei N. Silver nanoparticles and their applications. Applied Biology. 2013; 3(11): 13-31.
Kavyani B, Alikhani MY, Arabestani MR, Moradkhani SH & Taheri M. The effect of garlic extract on the expression of genes elastase andexotoxin A in Pseudomonas aeruginosa. Tehran University Medical Journal. 2016; 74(8): 584-590.
Elgayyar M, Draughon FA, Golden DA & Mount JR. Antimicrobial activity of Essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of Food Protection. 2001; 64(7): 1019-1024.
Pirigharaghie T & Sadatshandiz A. The Inhibitory Effects of Silver Nanoparticles on Bap Gene Expression in Antibiotic-Resistant Acientobacter bumanni Isolates using Real-Time PCR. Scientific Journal of Ilam University of Medical Sciences. 2018; 26(4): 175-185.
Tong Zh & et al. Modified protocol for RNA extraction from different peach tissues suitable for gene isolation and Real-Time PCR analysis. Molecular Biotechnology. 2012; 50(3): 229-236.
Azizi O & et al. Molecular analysis and expression of bap gene in biofilm forming multi drug-resistant Acinetobacter baumannii. Report of Biochemistry Molecular Biology. 2016; 5: 68-74.
Liesje S, Bart D, Paul V, Benny F & Pyck G. The Antibacterial Activity of Biogenic Silver and Its Mode of Action. World Journal of Microbialogy and Biotechnology. 2011; 31(2): 113-189.
Flockton, TR, Schnorbus L, Araujo A, Adams J, Hammel M & Perez LJ. Inhibition of Pseudomonas aeruginosa Biofilm Formation with Surface Modified Polymeric Nanoparticles. Pathogens. 2019; 8(2): 55.
Yuan YG, Peng QL & Gurunathan S. Effects of silver nanoparticles on multiple
drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa from mastitis-infected goats: An alternative approach for antimicrobial therapy. International journal of molecular sciences. 2017; 18(3): 569.
Darabpour E, Doroodmand MM, Halabian R & Imani Fooladi AA. Sulfur-Functionalized Fullerene Nanoparticle as an Inhibitor and Eliminator Agent on Pseudomonas aeruginosa Biofilm and Expression of toxA Gene. Microbial Drug Resistance. 2019; 25(4): 594-602.
Singh R, Nadhe S, Wadhwani S, Shedbalkar U & Chopade B. A nanoparticles for control of biofilms of Acinetobacter species. Multidisciplinary Digital Publishing Institute. 2016; 9(5): 383.
Hendiani S, Abdiali A, Mohammadi P. Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation. Biological Journal of Microorganism. 2014; 2(8): 51-56.
Palanisamy NK, Ferina N, Amirulhusni AN, Mohd-Zain Z, Hussaini J, Ping J & Durairaj R. Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa. Journal of nanobiotechnology. 2014; 12(2): 2-7
Camporotondi D, Foglia M, Alvarez G, Mebert A, Diaz L, Coradin T & Desimone M. Antimicrobial properties of silica modified nanoparticles. Microbial Pathogens and Strategies for Combating Them. Science, Technology and Education; Microbiology Book Series. 2013: 283-290.
Yeo Y, Ito T, Bellas E, Highley CB, Marini R & Kohane DS. In situ cross-linkable hyaluronan hydrogels containing polymeric nanoparticles for preventing postsurgical adhesions. Annals of surgery. 2007; 245(5): 819.
_||_Soltan DM & et al. Inhibitory effect of Lactobacillus plantarum and Lactobacillus fermentum isolated from the faeces of healthy infants against nonfermentative bacteria causing nosocomial infections. New Microbes New Infect. 2017; 15: 9-13.
Motaghi B & Mojafipour S. Outer Membrance Protein D Gene in clinical Isolates of Pseudomonas Aeruginosa and its Role in Antibiotic Resistance. Journal of Fasa University of medical Sciences. 2016; 5(4): 501-507.
Tang Y & et al. Genotyping of Pseudomonas aeruginosa Type III Secretion System Using Magnet ic EnrichmentMultiplex Polymerase Chain Reaction and Chemiluminescence. Journal of Biomedical Nanotechnology.2016; 12(4): 762-769.
Mokari M, Owlia P, Marashi SMA, Saderi H & Dehghan Zadeh Z. The effect of supernatant of Saccharomyces cerevisiae yeast on preventing the growth of Pseudomonas aeruginosa bacteria and its effect on exotoxin S gene expression in Pseudomonas aeruginosa bacteria by Real-Time PCR methodd. Daneshvar Medicine: Basic and Clinical Research Jouranl. 2018; 25(6): 67-74.
BouillotS,AttréeI & HuberP. Pharmacological Activation of Rap1 Antagonizes the Endothelial Barrier Disruption Induced by Exotoxins ExoS and ExoT of Pseudomonas aeruginosa. Infect Immun. 2015; 83(5): 1820-1829.
Pavlovskis OR, Iglewski BH & Pollack M. Mechanism of action of Pseudomonas aeruginosa exotoxin A in experimental mouse infections: adenosine diphosphate ribosylation of elongation factor 2. Infection and Immunity. 1978; 19(1): 29-33.
Donelli G & Vuotto C. Biofilm based infections in long term care facilities. Future Microbiology. 2014; 9: 175-188.
Tran QH, Nguyen VQ & Le A. Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Advances in Natural Sciences:Nanoscience and Nanotechnology. 2013; 4(3).
Mortazavi H, Nakhaei Moghaddam M & Abadi NS. Study of the Effect of Silver Nanoparticles on Biofilms Formation by Staphylococcus epidermidis. Journal of Rafsanjan University of Medical Sciences. 2015; 14(2): 125-136.
Björndahl MR, Cao LJ, Nissen S, Clasper LA, Johnson Y, Xue Z & et al. Lnsulin-like growth factors 1 and 2 induce lymphangiogenesis in vivo. Proceedings of the National Academy of Sciences. 2005; 102(43): 15593-15598.
Heinlaan M, Lvask A, Blinov L, Dubourgier HC & Kahur A. Toxicity of nanosized and bulk Zno, Cuo and Tio 2 to bacteria vibrio Fischer and crustaceans Daphnia magna and Thamnocephalus platyurus. Chemosphere. 2008; 71(7): 1308-1316.
Fernando S, Gunasekara T & Holton J. Antimicrobial Nanoparticles: Applications and mechanisms of action. Sri Lankan Journal of Infectious Diseases. 2018; 8(1): 2-11
Zargar M & Mohamadeibandarei N. Silver nanoparticles and their applications. Applied Biology. 2013; 3(11): 13-31.
Kavyani B, Alikhani MY, Arabestani MR, Moradkhani SH & Taheri M. The effect of garlic extract on the expression of genes elastase andexotoxin A in Pseudomonas aeruginosa. Tehran University Medical Journal. 2016; 74(8): 584-590.
Elgayyar M, Draughon FA, Golden DA & Mount JR. Antimicrobial activity of Essential oils from plants against selected pathogenic and saprophytic microorganisms. Journal of Food Protection. 2001; 64(7): 1019-1024.
Pirigharaghie T & Sadatshandiz A. The Inhibitory Effects of Silver Nanoparticles on Bap Gene Expression in Antibiotic-Resistant Acientobacter bumanni Isolates using Real-Time PCR. Scientific Journal of Ilam University of Medical Sciences. 2018; 26(4): 175-185.
Tong Zh & et al. Modified protocol for RNA extraction from different peach tissues suitable for gene isolation and Real-Time PCR analysis. Molecular Biotechnology. 2012; 50(3): 229-236.
Azizi O & et al. Molecular analysis and expression of bap gene in biofilm forming multi drug-resistant Acinetobacter baumannii. Report of Biochemistry Molecular Biology. 2016; 5: 68-74.
Liesje S, Bart D, Paul V, Benny F & Pyck G. The Antibacterial Activity of Biogenic Silver and Its Mode of Action. World Journal of Microbialogy and Biotechnology. 2011; 31(2): 113-189.
Flockton, TR, Schnorbus L, Araujo A, Adams J, Hammel M & Perez LJ. Inhibition of Pseudomonas aeruginosa Biofilm Formation with Surface Modified Polymeric Nanoparticles. Pathogens. 2019; 8(2): 55.
Yuan YG, Peng QL & Gurunathan S. Effects of silver nanoparticles on multiple
drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa from mastitis-infected goats: An alternative approach for antimicrobial therapy. International journal of molecular sciences. 2017; 18(3): 569.
Darabpour E, Doroodmand MM, Halabian R & Imani Fooladi AA. Sulfur-Functionalized Fullerene Nanoparticle as an Inhibitor and Eliminator Agent on Pseudomonas aeruginosa Biofilm and Expression of toxA Gene. Microbial Drug Resistance. 2019; 25(4): 594-602.
Singh R, Nadhe S, Wadhwani S, Shedbalkar U & Chopade B. A nanoparticles for control of biofilms of Acinetobacter species. Multidisciplinary Digital Publishing Institute. 2016; 9(5): 383.
Hendiani S, Abdiali A, Mohammadi P. Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation. Biological Journal of Microorganism. 2014; 2(8): 51-56.
Palanisamy NK, Ferina N, Amirulhusni AN, Mohd-Zain Z, Hussaini J, Ping J & Durairaj R. Antibiofilm properties of chemically synthesized silver nanoparticles found against Pseudomonas aeruginosa. Journal of nanobiotechnology. 2014; 12(2): 2-7
Camporotondi D, Foglia M, Alvarez G, Mebert A, Diaz L, Coradin T & Desimone M. Antimicrobial properties of silica modified nanoparticles. Microbial Pathogens and Strategies for Combating Them. Science, Technology and Education; Microbiology Book Series. 2013: 283-290.
Yeo Y, Ito T, Bellas E, Highley CB, Marini R & Kohane DS. In situ cross-linkable hyaluronan hydrogels containing polymeric nanoparticles for preventing postsurgical adhesions. Annals of surgery. 2007; 245(5): 819.
