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Open Access Article
1 - Prevalence study of Pseudomonas aeruginosa septicemia in broiler in Mazandaran
سیدمحمد حسینی تقی زهرائی صالحی مجتبی شالیزار مرتضی شعبانی عارف ابراهیم زاده شیلا امیدظهیرPseudomonas aeruginosa is the most common avian pathogens can cause localized or systemicdiseases in young and growing poultry. Morbidity and mortality varies from 2 to 100%, butmore commonly about 2-12% with greatest losses in very young birds. The disease occurred in6 MorePseudomonas aeruginosa is the most common avian pathogens can cause localized or systemicdiseases in young and growing poultry. Morbidity and mortality varies from 2 to 100%, butmore commonly about 2-12% with greatest losses in very young birds. The disease occurred in6-10 days-old ross broiler chickens. Clinical signs showed sleepy appearance, closed eyes, mildkeratitis and catarrhal rhinitis. Gross lesions revealed airsacculitis, congestion and swollen ofkidneys with deposition of ureats in the urters and proctitis. The disease morbidity and mortalityrates were 16.2% and 6%, respectively. Microbial culture was determined motile and Gramnegativebacteria, suspected greenish colonies, smell like fruity and beta hemolysis on bloodagar. Consequently, we suspected to Pseudomoniasis after culture and clinical signs finding.These isolates were transmitted to the department of microbiology, faculty of veterinarymedicine, university of Tehran and all isolates were identified by microbiological phenotypiccharacteristics with conventional methods and Pseudomonas aeruginosa was finally diagnosed. Manuscript profile -
Open Access Article
2 - Evaluation of metallo-betalactamase and carbapenemase activity in Pseudomonas aeruginosa strain isolated from clinical samples in Tehran
سمیه Mashayekhy فاطمه Noorbakhsh سحر Honarmand JahromiPseudomonas aeruginosa is an opportunistic pathogen and a major cause of nosocomial infections. Metallobetalactamases and carbapenemases are the most important factors in resistance to carbapenem drugs in Pseudomonas aeruginosa strains. The aim of this study was to eval MorePseudomonas aeruginosa is an opportunistic pathogen and a major cause of nosocomial infections. Metallobetalactamases and carbapenemases are the most important factors in resistance to carbapenem drugs in Pseudomonas aeruginosa strains. The aim of this study was to evaluate the activity of metallobetalactamase and carbapenemase in Pseudomonas aeruginosa isolated from clinical specimens. 49 strains of Pseudomonas aeruginosa isolated from patients admitted to the intensive care unit were identified by biochemical methods, then their antibiotic susceptibility was determined by Kirby- Bauer method. MBL producing strains were identified by phenotypic method combined disk test and KPC- producing strains were evaluated by MHT method. PCR method was also used to identify strains carrying VIM, SIM, GIM, SPM and IMP genes. Antibiotic resistance to ticarcillin, meropenem, gentamicin, ciprofloxacin, cefpime and ceftazidime were 89.8%, 51%, 44.9%, 67.4%, 93.9%, 95.9%, respectively. By phenotypic analysis combined disk test, 55.1% of the strains were identified as metallo-betalactamase producing strains. Also, 38.8% of carbapenemase producing strains were observed by MHT method. The frequencies of each of these gene’s VIM, SIM and GIM were 63.3%, 38.8%, 34.7%, respectively, and SPM and IMP genes were not observed in any of the strains in this study. Manuscript profile -
Open Access Article
3 - Investigating the Probability of Water Coolers Contamination with Pseudomonas Aeruginosa in a number of Tehran Hospitals
Samaneh Abdi Andrabi Reza Haji Seyed Mohammad Shirazi Seyed Amir Ali Anvar Maryam AtaeeBackground and Objective: Evaluation of the probability of water cooler contamination in hospitals to determine the water quality has important role in social safty. Pseudomonas Aeruginosa is considered as an opportunistic pathogen, since it is possible to grow with min MoreBackground and Objective: Evaluation of the probability of water cooler contamination in hospitals to determine the water quality has important role in social safty. Pseudomonas Aeruginosa is considered as an opportunistic pathogen, since it is possible to grow with minimal nutritional requirements, it can grow in water bodies containing very little food such as tap water. This ability has led to their continuous presence in the hospital environment. The main objective of this study was to determine the rate of contamination in few of Tehran's hospitals water-cooling devices in terms of contamination with Pseudomonas Aeruginosa. Method: This is an experimental study and the stages of work were based on observation and test in the laboratory. Water samples were sampled in two seasons (autumn and winter) in 100 samples from five hospitals in different regions of Tehran. At first, the samples were transferred to the laboratory in a compartment filled with ice gel at 4 °C, and then the tubes containing different media were kept in an incubator at 37 °C for 24 to 48 hours. Then, the samples were examined for nutritional needs in terms of growth rate, carbohydrate usage by possible bacteria through oxidation or fermentation, glucose fermentation pathways, and determination of the bacterial power in the use of citrate, the presence of enzyme oxidase and catalase, the presence or absence of Enterobacteriaceae species and Salmonella was examined using culture media and specific tests. Data analysis was done descriptively using excel and SPSS software. Findings: As a result of the research Bacillus species were identified. Also, in the tested specimens, pseudomonas susceptible to Aeruginosa species and the suspected alcaligenes species of Faecalis were also identified. Discussion &Conclusion: The temperature has a significant effect on the growth of the Pseudomonas Aeruginosa, and a high temperature of 37 °C leads to the increase in growth rate, and in low temperature of 4 °C during sunlight in cold weather (autumn and winter), the bacteria could not be detected in water coolers Manuscript profile -
Open Access Article
4 - The Comparative Study of telluride removal by a moderately halophilous bacterium, Halomonas elongate, a halotolerant bacterium, Enterococcus faecalis and a non-halophilous bacterium, Pseudomonas aeruginosa
Zeynab Sadat Motesharrei Mohammad Ali AmoozegarHalophilous, halotolerant and non-halophilous microorganisms have important roles in bioremediation and transformation of toxic metal compounds in polluted environments. Identification of toxic metal-tolerant strains and their removal patterns are the first steps of app MoreHalophilous, halotolerant and non-halophilous microorganisms have important roles in bioremediation and transformation of toxic metal compounds in polluted environments. Identification of toxic metal-tolerant strains and their removal patterns are the first steps of applying them in the bioremediation processes. In the current study, telluride removal in three moderately halophilous, halotolerant and non-halophilous bacteria, Halomonas elongate DSM 2581, Enterococcus faecalis PTCC1237 and Pseudomonas aeruginosa PTCC1074, respectively, and the effects of selenooxyanions and arsenate on this removal, were determined by means of a colorimetric method using di-ethyl thio-carbamate. The strains H. elongate, E. faecalis and Ps. aeruginosa showed the maximum MIC to telluride as 0.25, 8 and 0.25 mM, respectively. In halophilous microorganism, H. elongata, selenooxyanions had a positive effect on increasing the telluride resistance, since K-telluride plus 25 mM Na-Selenite or Na-selenate were added together into the medium, tolerance to telluride was increased from 0.25 mM to 18 mM and from 0.25 mM to 3 mM, respectively , telluride tolerance in halotolerant strain ,E. faecalis, in the presence of 25 mM Se (IV) or 5 mM Se (VI) or 0.1 mM As (V) was increased and reached to 80 mM , 16 mM and 16 mM , respectively,but in Non-halophilous bacterium, Ps. aeruginosa adding 25 mM Se (IV) or 5 mM Se (VI) or 5 mM As (V) did not cause increasing of MIC of telluride. The ability of halophilous strain to telluride removal was increased in comparison of control condition (medium containing of just telluride) by 48/21% and 25.74% , when 0.1 mM Te(IV) plus 50 mM Se (IV) or 1 mM Se (VI) was used respectively. 0.2 mM Te (IV) plus 1 mM Se (IV) or 1 mM Se (VI) or 0.1 mM As (V) in E. faecalis can give rise 2.2% , 7.33% and 75% increase in Telluride removal, as well. The most positive effect of Selenite and Selenate in Telluride removal was observed in H. elongata and the lowest of it was observed in E. faecalis. A unique characteristic of E. faecalis in removing of Tellurite by 75% in the presence of Arsenate was the thing which observed only in E. faecalis but not in two other bacteria studied here. In the case of P. aeruginosa, 8 mM Se (IV) or 1 mM Se (VI) plus 0.1 mM Te (IV) can also cause higher increase in telluride removal by 34.35% and 11.82%, respectively. X-ray fluorescence (XRF) studies and scanning transmission electron microscopy examination using an EDS analysis system, demonstrated that the black and red deposits formed in bacterial cells, was metallic tellurium and selenium Manuscript profile -
Open Access Article
5 - Determine the antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from raw milk
faeze zadsafar MOHSEN ZARGAR سید سهیل آقاییAbstractBackground and Objectives: Pseudomonas aeruginosa, a pathogen bacteria, that is resistance to antibiotics and can be used in patients with immune deficiency and cause disease. This study aimed to determine the antimicrobial susceptibility of Pseudomonas aerugino MoreAbstractBackground and Objectives: Pseudomonas aeruginosa, a pathogen bacteria, that is resistance to antibiotics and can be used in patients with immune deficiency and cause disease. This study aimed to determine the antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from raw milk and multiple resistance of the isolates and ESBL isolates.Method: Initially, 117 Pseudomonas aeruginosa isolates from dairy products were confirmed by the standard methods of microbiology and then evaluated the antibiotic resistance of 10 strains against 10 antibiotic which selected from different categories according to the CLSI standard. Results: In the study of antibiotic resistance Ceftazidime antibiotic showed most Antibiotic resistance and amikacin antibiotic polymyxin B antibiotic resistance and lowest, respectively.27 isolates (23%), multiple drug resistance (MDR), the three classes of antibiotics showedand 86 isolates were resistant to the ceftazidime antibiotic (73.5%) isolates, of which 28 were positive ESBL (32.55%). Conclusion: Due to high prevalence Pseudomonas aeruginosa in raw milk, and existence of antibiotic resistance genes in bacteria, it is necessary to apply appropriate strategies for livestock health control, to prevent the spread of bacteria. Manuscript profile -
Open Access Article
6 - Molecular detection of DHA beta-lactamase gene in Pseudomonas aeruginosa isolated from skin samples in Qom
Horisadat Hejazi Mohsen Zargar mehrdad mamarianIntroduction Pseudomonas aeruginosa can infect virtually all tissues in the body. this bacteria can be colonized in burns, wounds, corneal tissue, urinary tract and lungs, and after entering blood, cause septicemia or localized lesions in other parts of the body. Today, MoreIntroduction Pseudomonas aeruginosa can infect virtually all tissues in the body. this bacteria can be colonized in burns, wounds, corneal tissue, urinary tract and lungs, and after entering blood, cause septicemia or localized lesions in other parts of the body. Today, due to the untapped use of effective drugs On the pseudomonas aeruginosa, this bacterium is strongly resistant to antibiotics. The purpose of this study was to find the gene for resistance to beta-lactams in pseudomonas aeruginosa isolated from skin samples. materials and methods: A total of 100 isolates of Pseudomonas were used in Microbiology Bank of Microbiology Research Laboratory of Islamic Azad University of Qom. After cultivation and biochemical confirmation of isolates, genomic extraction was performed first to detect these genes and then detected by PCR with DHA genes. Results: 45 isolates resistant to imipenem; 22 cases were positive in the double-synergistic disks. Six isolates were positive for DHA gene. Conclusion: Increasing resistance to Pseudomonas aeruginosa causes problems in treating patients and a periodic review of drug allergy for inappropriate treatment. Manuscript profile -
Open Access Article
7 - Using a phenotypic method to confirm the presence of the efflux pump associated with resistance to ciprofloxacin in Pseudomonas aeruginosa isolated from skin samples
Ali Rahmati Mohammad Reza zolfagharyIntroduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the de MoreIntroduction One of the most important causes of nosocomial infections in patients with skin infections is Pseudomonas aeruginosa. The occurrence of antibiotic resistance with various mechanisms is one of the problems in treating these patients. The main role in the development of multiple resistance to antimicrobial drugs is played by the MexAB efflux pumps. The aim of this study was to evaluate the presence of the MexAB efflux pumps in the Pseudomonas aeruginosa isolated from skin samples. Materials and Methods: In this study 200 swab specimens from patients with skin infections were collected in Qom hospitals. Pseudomonas aeruginosa specimens were confirmed by biochemical tests. The drug susceptibility pattern was compared to ciprofloxacin by disk diffusion method and the phenotypic study of the effects of efflux pumps was done using cartwheel method . Results: In this study, 89 (45%) samples were infected with Pseudomonas aeruginosa, of which 19 cases (21%) were resistant to ciprofloxacin and more than 54% of the presence of the pump in the Pseudomonas aeruginosa more than 54% of the presence of the pump in the Pseudomonas aeruginosa. Conclusion: the presence of more than 54% of the presence of the pump in the Pseudomonas aeruginosa, is important in order to suggest a suitable therapeutic model for the infected patients. Keywords: Pseudomonas aeruginosa, skin infections, efflux pump Manuscript profile -
Open Access Article
8 - The effect of silver nanoparticles on the expression of exotoxin A and S genes in Pseudomonas aeruginosa
Muhammad Hemati Zahra Keshtmand Katayoun BorhaniBackground: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been wide MoreBackground: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections and exotoxin genes are important in the development of this infection.On the other hand, with the advancement of nanotechnology, today, the use of nanoparticles has been widely considered in the field of medicine and medicine, especially against microbes. The aim of the present study was to investigate the effect of silver nanoparticles (AgNPs) on the expression of Pseudomonas aeruginosa exotoxin A and S genes. Methods: In this experimental study, the minimum growth inhibition concentration of silver nanoparticles was determined by microdilution method. First, the lowest concentration of bacterial growth inhibitor is determined with silver nanoparticles. Then, at a concentration lower than the lowest growth rate, the expression of Pseudomonas aeruginosa exotoxin A and S genes was examined by Real time PCR. Data were analyzed using one-way ANOVA, Tukey test and P value less than 0.05. Findings: Silver nanoparticles with a concentration of 62.5 μg / ml had growth inhibitory effects on Pseudomonas aeruginosa. Also, at a concentration of 31.25, the expression of Pseudomonas aeruginosa exotoxin A and S genes was significantly reduced (p < 0.05). Conclusion: The results of this study show that lower concentrations (31.25 μg/ml) than growth inhibition concentrations (62.5μg/ml) can reduce the expression of Pseudomonas aeruginosa exotoxin A and S genes. Manuscript profile -
Open Access Article
9 - A review of biofilm and chorus sensing formation in Pseudomonas aeruginosa
Rezvaneh behzadmehr Maryam Beigomi saeide saeidiPseudomonas aeruginosa is an opportunistic pathogen and causes 10 to 15% of nosocomial infections. The presence of unity genes is one of the most important invasive mechanisms of Pseudomonas aeruginosa and this issue is of special medical importance.The expression of ma MorePseudomonas aeruginosa is an opportunistic pathogen and causes 10 to 15% of nosocomial infections. The presence of unity genes is one of the most important invasive mechanisms of Pseudomonas aeruginosa and this issue is of special medical importance.The expression of many Pseudomonas aeruginosa pathogenic genes is controlled by a gene system called the Quorum Sensing System (QS). Chromosensing is a cell-to-cell communication system using small SMs molecules in single-celled organisms. The present review study was obtained with the help of articles indexed in Magiran and SID Persian scientific databases and Scopus, Google scholar, Ebscohost and Science Direct English databases.The QS system genes are highly abundant among human-derived Pseudomonas aeruginosa strains. It is also known as an infectious bacterium with high antibiotic resistance.The results showed that medicinal plants in different dilutions inhibit chromosome sensing and biofilm in bacteria.The results showed that medicinal plants in different dilutions inhibit chromosome sensing and biofilm in bacteria. Manuscript profile -
Open Access Article
10 - Biofilm Formation and Determination of Antimicrobial Resistance Pattern in Pseudomonas aeruginosa Clinical Isolates
Maasuomeh Mahdavi Ourtakand Sahar Honarmand Jahromi Zahra ResalatiPseudomonas aeruginosa is one of the most important pathogens in hospitals. One of the therapeutic problems of this bacterium is antibiotic resistance to common antibiotic treatments associated with biofilm production. This study was conducted to find out the relationsh MorePseudomonas aeruginosa is one of the most important pathogens in hospitals. One of the therapeutic problems of this bacterium is antibiotic resistance to common antibiotic treatments associated with biofilm production. This study was conducted to find out the relationship between antibiotic resistance and biofilm formation on P. aeruginosa isolated from 50 clinical specimens of patients referring to Milad Hospital in Tehran. Biochemical and differential tests were carried out to identify and isolate P. aeruginosa. The resistance of the strains was determined by antibiogram test and the ability of biofilm strain formation was investigated by microtiterplate method. The results showed that the highest resistance to antibiotics was ticarcillin (48%) and imipenem (36%) and 17 strains (34%) had multiple drug resistance. Also, the ability to form biofilms of strains showed that 52% of them had strong biofilms and among 17 strains of P. aeruginosa with multiple drug resistance, 12 strains had the ability to form a strong biofilm. According to the results, a significant percentage of MDR strains have the ability to produce strong biofilms. Regarding the role of biofilms in reducing the penetration of the drug into cell, biofilm-producing bacteria have a high drug resistance, which is a warning to the medical community. Manuscript profile -
Open Access Article
11 - Evaluation of the effect of gold nanostructure on the production of bio surfactants from Pseudomonas aeruginosa
Yaser Ahsani Arani Zahra Noormohammadi behnam rasekh fatemeh Yazdian Hojjat kazemi Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this s More Raminolipids belong to the group of glycolipid bio surfactants and were first isolated from Pseudomonas aeruginosa. Rhamnolipids are a good alternative to synthetic surfactants due to their low toxicity, biodegradability and selective performance. In this study, the effect of gold (Au) nanoparticles on the growth and production of Pseudomonas aeruginosa PBCC5 bio surfactant was evaluated. Different concentrations of 1, 500 and 1000 mg / l nanoparticles were used. In this study, bio surfactant surface tension and emulsification indices (24E) were measured. The presence of nanoparticles on the bacterial surface was investigated by TEM and the morphology of nanoparticles was investigated by SEM. Binding of nanoparticles to bio surfactants was confirmed by TEM. The results showed that gold nanoparticles were not bactericidal and also increased bacterial growth and bio surfactant production. The surface tension of all samples was reduced from 72 mN / m distilled water to 35-32 mN / m. Manuscript profile -
Open Access Article
12 - Antibacterial activity of zinc aluminate nanoparticles against foodborne pathogenic bacteria of E. coli and P. aeruginosa
A. Taheri M. Ziaadini M. GahramzeiMaterials in nanoscale dimensions have a high surface-to-volume ratio, and thus have better reactions with other materials. The development of nanotechnology over the past decades has opened up opportunities for discovering the antibacterial effects of metallic nanopart MoreMaterials in nanoscale dimensions have a high surface-to-volume ratio, and thus have better reactions with other materials. The development of nanotechnology over the past decades has opened up opportunities for discovering the antibacterial effects of metallic nanoparticles. In this study, the antibacterial properties of nanoparticles on aluminate (ZnAl2O4) were studied on Escherichia coli and Pseudomonas aeruginosa bacteria. Antibacterial activity was assessed by using the disk diffusion method on agar using nanoparticles on synthesized aluminate. The non-growth halo diameter indicates the sensitivity of bacteria to antimicrobials. The minimum inhibitory concentration of bacterial growth (MIC) and minimum bacterial leaching concentration (MBC) was measured by microdilution in sterile tubes. Statistical analysis was done by T-test. The results of this study showed that Pseudomonas aeruginosa sensitivity (16.06±1.8mm) was higher than E. coli (11.64±2.2mm) (p < 0.05). The minimum inhibitory concentration (MIC) and bactericidal concentration MBC of bacteria Pseudomonas aeruginosa and Escherichia coli bacteria MIC and MBC was lacking. According to the results obtained in this study, we conclude that Pseudomonas aeruginosa was more sensitive to alumina than that of E. coli against nanoparticles. Manuscript profile -
Open Access Article
13 - A comparative study of histopathological effects of aqueous extract of cinnamon and honey with sulfadiazine on skin burn wound healing in rats infected with Pseudomonas aeuroginosa
Mohammadreza Valilou Sevda ValilouPseudomonas aeruginosa, a ubiquitous opportunistic pathogen, is the most common infective agent of burn wounds. The aim of this study was to compare the histopathological effect of a mixture of aqueous extract of cinnamon and honey with silver sulfadiazine on the healin MorePseudomonas aeruginosa, a ubiquitous opportunistic pathogen, is the most common infective agent of burn wounds. The aim of this study was to compare the histopathological effect of a mixture of aqueous extract of cinnamon and honey with silver sulfadiazine on the healing of Pseudomonas aeruginosa infected second grade skin burn wounds in rats. To this end, 60 male rats were randomly assigned to four experimental groups (15 rats in each group). After inducing anesthesia, second grade burn wound with the diameter of 12 mm was created in the dorsal region of rats. Then, 1.5×108 cfu/ml P. aeruginosa PA01was equally bestrewed on the wound of all rats. Every 12 hours, silver sulfadiazine (group 1), honey (group 2) and aqueous extract of cinnamon and honey (group 3) were applied to the wounds and group 4 was kept as control. On days 7, 14, and 21, five rats were selected from each group at each time point and after inducing anesthesia and measuring the diameter of the wound by coliseum, microbial and histopathological samples were taken from the wounds. Microbial studies showed that in all groups except the control group, the growth of the microbe was stopped. Histopathological observations regarding wound healing and diameter showed that there was a significant difference between treatment groups and the control group on days 7, 14 and 21 (p<0.05). On the 21st day, the extract of cinnamon and honey group had superior wound healing than silver sulfadiazine group but inferior in comparison to honey. According to the results btained, it is possible to utilize honey and mixture of aqueous extract of cinnamon and honey for treatment of infected skin burn wounds. Manuscript profile -
Open Access Article
14 - The reaction of Eryngium campestre plant to glycine, nitroxine and humic acid and investigating the effect of its extract on dealing with Staphylococcus aureus and Pseudomonas aeruginosa bacteria
Zeinab Nasrollahnejad Ghomi khodayar hemati Mehrdad BabarabieEryngium campestre is a biennial herbaceous plant native to northern Iran. Nowadays, the use of chemical fertilizers has increased significantly, which has irreparable effects on the environment and human health. The use of organic compounds and biofertilize MoreEryngium campestre is a biennial herbaceous plant native to northern Iran. Nowadays, the use of chemical fertilizers has increased significantly, which has irreparable effects on the environment and human health. The use of organic compounds and biofertilizers instead of chemical fertilizers has recently received much attention. Also, it is more important to study about natural substances that contain more antimicrobial properties and less side effects, such as plant extracts. For this purpose, an experiment was conducted. This experiment was conducted in Gorgan University of Agricultural Sciences and Natural Resources in 2019-2020 in a factorial format based on a completely randomized design with 3 replications. The treatments included: nitroxin (0, 1 and 2 ml/l), humic acid (0, 1 and 2 ml/l), glycine (0, 75 and 150 mg/l). The measured traits included total phenol, total flavonoid, antioxidant activity, total sugar, chlorophyll, carotenoid and activity of catalase and peroxidase enzymes. The effect of glycine on the measured traits was significant except flavonoids and catalase enzyme activity at 5 and 1% levels. Also, the effect of nitroxin on the measured traits was significant at the level of 1%. The effect of humic acid on the measured traits was significant except for the amount of antioxidant activity, chlorophyll and catalase enzyme activity at the level of 5 and 1%. Also, the interaction effect of all three treatments on the measured traits except catalase enzyme was significant at 1% level. In general, the results showed that the treatments used in this research, individually and in combination (interaction effect), increased most of the measured traits. Also, an experiment was conducted regarding the effect of Eryngium campestre extract on the population of Pseudomonas aeruginosa and Staphylococcus aureus bacteria. The results showed that Eryngium campestre extract did not inhibit the bacteria. In general, the results showed that the used compounds can be used to improve the biochemical characteristics of the Eryngium campestre. Manuscript profile -
Open Access Article
15 - In silico Analysis of Inhibitory Potential of Major non-Steroidal Anti-inflammatory Drugs against Las-quorum Sensing Circuit in Pseudomonas aeruginosa
Hossein Zahmatkesh Behnam RastiThe emergence of drug resistance, therapeutic failure, and the development of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS) dependent virulence factors. The antimicrobial potential of some non-steroidal anti-infl MoreThe emergence of drug resistance, therapeutic failure, and the development of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS) dependent virulence factors. The antimicrobial potential of some non-steroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, has been determined in laboratory studies. Herein, a docking analysis was conducted to examine the interaction between seven NSAIDs and the proteins of the Las system. Initially, the three-dimensional structure of selected NSAIDs (Diclofenac sodium, Ibuprofen, Ketoprofen, Mefenamic acid, Meloxicam, Naproxen, and Tenoxicam), and natural ligand of LasR (3-oxo-C12-HSL) were retrieved from PubChem database. Also, crystal structures of LasI Synthase and transcriptional activator protein LasR were obtained from Protein Data Bank. Subsequently, the molecular docking analysis utilizing AutoDock Vina software was employed to investigate the capability of the selected NSAIDs to inhibit the LasI/LasR receptor. Based on our findings, the majority of the selected NSAIDs exhibited favorable interactions with LasI/R proteins. Moreover, ketoprofen exhibited the strongest interactions with both proteins. In summary, this work suggested that NSAIDs, especially ketoprofen and naproxen, have promising potential as candidates for further in vitro and in vivo investigations to inhibit the QS circuits of P. aeruginosa. Manuscript profile -
Open Access Article
16 - Biological Removal of Zinc from Automotive Hazardous Paint Sludge: a comparison between an endogenous Pseudomonas aeruginosa isolateand Acidithiobacillus thiooxidans PTCC1692
Fatemeh Honarjooy Barkusaraey Roya Mafigolami Gholam KhayatiResearch on the hazardous waste of paint sludge is mostly focused on recovering the sludge and pollution control with methods such as solidification. This research aimed to study the comparison of biological removal of zinc from paint sludge using a native Pseudomonas a MoreResearch on the hazardous waste of paint sludge is mostly focused on recovering the sludge and pollution control with methods such as solidification. This research aimed to study the comparison of biological removal of zinc from paint sludge using a native Pseudomonas aeruginosa strain and Acidithiobacillus thiooxidans PTCC 1692. Samples of water-based paint sludge were collected under aseptic conditions. Isolation of endogenous strains was performed in the Bushnell-Haas (BH) medium from paint sludge, and A. thiooxidans PTCC 1692 was cultured in the medium 119. Optimization of the process parameters for the biological removal of zinc was performed using the design of the experimental (DOE) method for both organisms. The results showed that A. thiooxidans is capable of biological removal of zinc with a higher pulp density and with a more extended bioleaching period. The results of the chemical analysis show the removal of zinc metal by 57.76% compared to P. aeruginosa by 36.39%, and, the removal of other metals such as Cr, Ni, Pb, and Cd were observed in the residual sludge from the process. Energy Disperse X-ray analysis (EDX) showed the more favorable effect of this bacterium in reducing heavy metals. Manuscript profile -
Open Access Article
17 - Investigating the Severity of Environmental Stress Conditions on Growth, the Protein Profile and Biochemical Characteristics of Pseudomonas aeruginosa ATCC 27853
Azita Tisheyar Moahmmad Faezi Ghasemi Nour AmirmozafariPseudomonas aeruginosa is the main cause of nosocomial infections, and is resistant to most antibiotics, this study aimed to evaluate the effects of different stress conditions on growth, the protein profile, and biochemical characteristics of this bacterium. The cells MorePseudomonas aeruginosa is the main cause of nosocomial infections, and is resistant to most antibiotics, this study aimed to evaluate the effects of different stress conditions on growth, the protein profile, and biochemical characteristics of this bacterium. The cells of P. aeruginosa ATCC 27853 in the logarithmic phase were exposed to different stress factors such as sucrose concentration, ethanol, acid, osmotic pressure, and CoCl2. Following each stress condition, the growth and the survival of bacterial cells were determined. Microscopic observation showed morphological changes in different stress conditions. P. aeruginosa ATCC 27853 tolerated up to pH 3, 55% (V/V) ethanol, and CoCl2 up to 7% (W/V), and beyond these amounts, the bacterium lost its ability to survive. Maximum tolerance to sucrose was about 35% (W/V). The results showed that different stress conditions could not effect on the main biochemical characteristics of P. aeruginosa ATCC 27853. Scanning electron microscopy of the cells exposed to different stress conditions showed wide changes in the morphology of cells. In addition, upon treatment of different stresses, significant changes were observed in the protein profile of P. aeruginosa ATCC 27853 according to SDS-PAGE analysis. It can be concluded that severe environmental stresses have great effects on the growth pattern, phenotypic characteristics, and protein profile of P. aeruginosa ATCC 27853. If the stresses are induced all at once, they will cause death, but if they are affected slowly and for a longer period, most bacteria will be able to repair the damaged parts, and the growth of will resume. Manuscript profile -
Open Access Article
18 - The Impact of Rhizospheric Pseudomonas aeruginosa on the Growth of Melissa officinalis
Elham Karami Monir Doudi Zahra Rezayatmand Ladan Rahimzadeh TorabiMicrobial communities occupy a significant position in the functioning and productivity of agricultural ecosystems. The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually beneficial partnerships with different plants. The ob MoreMicrobial communities occupy a significant position in the functioning and productivity of agricultural ecosystems. The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually beneficial partnerships with different plants. The objective of this study was to ascertain and isolate Pseudomonas strains that were obtained from the rhizosphere soil of M. officinalis. Furthermore, an examination was conducted on the morphological characteristics of M. officinalis that had been treated with standard and rhizospheric Pseudomonas. The experimental treatments consisted of 3 different groups: a rhizosphere Pseudomonas inoculation with a concentration of 106 and 109 CFU /ml, standard Pseudomonas inoculation with a concentration of 106 and 109 CFU /ml, and a control group that did not contain any bacteria. To effectively separate bacteria, specialized culture mediums, such as the King B medium, were employed. The biochemical and molecular results revealed that the isolated strain was Pseudomonas aeruginosa. The standard Pseudomonas treatment with a concentration of 109 CFU/ml exhibited the greatest root length of 24.1 cm, while the treatment lacking the presence of Pseudomonas displayed the lowest root length of 12.9. Treatments with rhizospheric P. aeruginosa showed the greatest values for stem length, as well as root and stem weight. Meanwhile, the control treatment resulted in the lowest measurements for these mentioned characteristics. Manuscript profile -
Open Access Article
19 - Antimicrobial and Anti-biofilm Effect of Samarium Oxide Nanoparticles on Pseudomonas aeruginosa Isolated from Hospital wards
Hossein Zahmatkesh Mirsasan Mirpour Hojjatolah Zamani Behnam RastiThe development of drug resistance, treatment ineffectiveness, and the occurrence of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS)-dependent virulence factors. This study aimed to synthesize samarium oxide nanopa MoreThe development of drug resistance, treatment ineffectiveness, and the occurrence of Pseudomonas aeruginosa infections are primarily attributed to biofilm formation and quorum sensing (QS)-dependent virulence factors. This study aimed to synthesize samarium oxide nanoparticles (Sm2O3NPs) using curcumin and evaluate their antimicrobial effects against P. aeruginosa isolated from different hospital wards. Based on TEM, XRD, and EDX analysis, the synthesized nanoparticles exhibited a cubic structure with an average size of 32.61 nm and a pure crystalline phase. The Sm2O3NPs exhibited notable antibacterial efficacy against P. aeruginosa strains. Based on the CV staining assay, sub-MIC of Sm2O3NPs inhibited the biofilm formation of P. aeruginosa strains by 16-69%. Additionally, the levels of pyocyanin in the NPs-treatment groups decreased by 29-58% compared to the control group. This research confirms the significant antibacterial potential of Sm2O3NPs, implying their potential utilization as an effective antibacterial agent against P. aeruginosa strains isolated from various hospital wards, after further characterization. Manuscript profile -
Open Access Article
20 - A review of Pseudomonas aeruginosa in the environment: pathogenesis, antibiotic resistance, genetic diversity, and removal ways from water treatment
Ghasem Ghorbani Ebrahim Rahimi Amir ShakerianPseudomonas aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Due to the fact that P. aeruginosa is an op MorePseudomonas aeruginosa as a waterborne pathogen is a growing concern to public health sectors. Many sources of environmental water could potentially be acting as a reservoir for potentially pathogenic strains of P. aeruginosa. Due to the fact that P. aeruginosa is an opportunistic pathogen and causes serious problems, it seems necessary to know the pathogenesis, antibiotic resistance, genetic diversity and methods of removing it from drinking water. The pathogenesis of P. aeruginosa has been extensively studied and proven to be a multifactorial process, mediated by quorum sensing. P. aeruginosa possess two quorum sensing systems, las and rhl that facilitate cell to cell communication through production of signalling molecules termed autoinducers to target specific receptors for activation. P. aeruginosa is intrinsically resistant to various antibiotics due to a low permeability in the outer membrane, which acts as a selective barrier. However, this bacterium is a highly diverse pathogen that is capable of adaptation to the surrounding environment. When subjected to antibiotic selective pressure, the induced response facilitates bacterial survival and develops antibiotic resistance. literature suggests that the large genome size and genome complexity are responsible for the ability of this bacterium to adapt and thrive in a diverse range of environments. The presence and persistence of these bacteria in environmental waters may pose a great risk to the public health and requires further work to fully characterize and quantify the input of MDR P. aeruginosa strains from the hospitals compared with those originating from the general community or other wastewater related sources. Manuscript profile -
Open Access Article
21 - Prevalence and antimicrobial resistance pattern of Pseudomonas aeruginosa strains isolated from rose water and herbal distillates in Kashan, 2018
Reza sharafati chaleshtori navid Mazroii Arani Elahe Alizadeh Alireza EtemadiPseudomonas aeruginosa (P. aeruginosa) is an opportunistic gram-negative and pathogenic bacterium that can be transmitted through food to humans. Antibiotic-resistant infections of this bacterium are a severe public health issue. This study aimed to determine the preval MorePseudomonas aeruginosa (P. aeruginosa) is an opportunistic gram-negative and pathogenic bacterium that can be transmitted through food to humans. Antibiotic-resistant infections of this bacterium are a severe public health issue. This study aimed to determine the prevalence and antibiotic resistance pattern of P. aeruginosa strains isolated from rose water and herbal distillates in Kashan. In this cross-sectional study, 400 rose water and herbal distillate samples were randomized purchased of local markets in Kashan and evaluated for the occurrence of P. aeruginosa by culturing methods. The obtained isolates were subjected to the disc diffusion antimicrobial susceptibility tests. Out of 400 samples, 16 (4 %) samples were contaminated withP. aeruginosa. The 100 % of isolates were resistant to ticarcillin, ceftazidime, colistin, and gentamicin. Also, the 13 (81.25 %) samples were sensitive to imipenem. The results showed a high occurrence of the antibiotic resistance P. aeruginosa strains isolated from rose water and herbal distillates. Manuscript profile -
Open Access Article
22 - Prevalence, antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains isolated from raw and frozen meat
Manizhe Rezaloo Abbasali Motalebi Zohreh Mashak Seyed Amir Ali AnvarPseudomonas aeruginosa strains are considered as the main causes of food spoilage and occasionally foodborne diseases. The present study was performed to evaluate the pattern of antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains i MorePseudomonas aeruginosa strains are considered as the main causes of food spoilage and occasionally foodborne diseases. The present study was performed to evaluate the pattern of antibiotic resistance and frequency of virulence factors in Pseudomonas aeruginosa strains isolated from meat. A total of 120 samples of raw and frozen meat were collected. Samples were evaluated for the presence of Pseudomonas aeruginosa using conventional microbial culture. The pattern of antibiotic resistance of Pseudomonas aeruginosa isolates was evaluated using the disk diffusion method. Genomic DNA was extracted from Pseudomonas aeruginosa isolates and the frequency of virulence factors was assessed using the polymerase chain reaction. Eleven out of 120 meat samples (9.16%) were contaminated with Pseudomonas aeruginosa. The prevalence of Pseudomonas aeruginosa in raw and curled meat samples was 5.00 and 13.33%, respectively. There was a statistically significant difference in the prevalence of Pseudomonas aeruginosa between raw and frozen meat samples (P <0.05). Pseudomonas aeruginosa isolates had the highest rate of antibiotic resistance against ampicillin (100%), penicillin (90.90%) and tetracycline (81.81%). The prevalence of resistance to imipenem (9.09%) and trimethoprim (18.18%) antibiotics was lower than other cases. ExoU (54.54%) and exoT (18.18%) genes were the most frequent virulence factors detected. The simultaneous presence of virulence factors and antibiotic resistance in Pseudomonas aeruginosa strains isolated from raw and frozen meat increases the need for further studies to confirm the role of this bacterium as an important food-spoilage bacterium. Manuscript profile -
Open Access Article
23 - Evaluation of anti-biofilm effect of aqueous-alcoholic extract of black pepper in vitro and food model
saeed akbari zohreh didar Mohsen Vazifedoost Bahare Hajirostamloo Mahnaz MohtashamiThe present study aimed to investigate the chemical composition of black pepper (Piper nigrum) aqueous-alcoholic extract and focus on its possible anti-biofilm activity against the two bacteria Bacillus subtilis and Pseudomonas aeruginosa in vitro and in simulated condi MoreThe present study aimed to investigate the chemical composition of black pepper (Piper nigrum) aqueous-alcoholic extract and focus on its possible anti-biofilm activity against the two bacteria Bacillus subtilis and Pseudomonas aeruginosa in vitro and in simulated conditions of the food model. Chemical analysis was used to identify the chemical composition of black pepper extract by mass spectrometry gas chromatography (GC - MS). The anti-biofilm activity of black pepper was measured using an automatic ELISA reader at 570 nm wavelength. The GC - MS analysis results indicated that the major compounds in black pepper extract include d-3-Carene (20.69%), Limonene (18%), Caryophyllene (17.90%) and b-Pinene (10.52%). According to the results in this study, black pepper aqueous- alcoholic extract with a 50% concentration had the highest effect in preventing the biofilm formation of both bacteria In addition, there was no significant difference between black pepper extract (50%) and peracetic acid 0/5% in the antibiofilm activity. There was also no significant difference in the type of bacterial growth media (TSB culture and fish extract) on bacterial biofilm formation in the presence of black pepper extract. Manuscript profile -
Open Access Article
24 - Prevalence of Pseudomnas aeruginosa in various species of shrimps of Isfahan and Chabahar in summer and autumn and the effect of season and place of supply on it
Seyedmajid Hashemi Ebrahim Rahimi Seyed Amirali Anvar Hamed Ahari Maryam AtaeePseudomonas aeruginosa a potential risk factor for food poisoning especially in aquatic foods like shrimp. In order to determine and compare the rate of infection in different species of shrimp in two seasons of summer and autumn in Isfahan and Chabahar cities, the abdo MorePseudomonas aeruginosa a potential risk factor for food poisoning especially in aquatic foods like shrimp. In order to determine and compare the rate of infection in different species of shrimp in two seasons of summer and autumn in Isfahan and Chabahar cities, the abdominal muscle of 35 shrimps in each season of each city were sampled. Samples were homogenized in peptone water medium, then incubated and cultured in PCA medium. Suspected colonies were isolated and then confirmatory tests of Pseudomonas aeruginosa and confirmation of the presence of nanI, the specific gene of Pseudomonas aeruginosa by PCR.In summer, in Isfahan and Chabahar, 48.5% and 25.7% were infected respectively. In autumn, the prevalence was 22.9% for Isfahan and 20% for Chabahar, respectively. In Isfahan, the highest rate of infection was related to Metapenaueus affinis and the lowest to Penaeus semisulcatus in summer. In autumn, the highest number of infected samples was related to Penaeus merguiensis and the lowest to Penaeus semisulcatus. In Chabahar city, in both seasons, the most infected samples were related to Metapenaeus affinis, and the lowest to Panaeus semisulcatus and in autumn was related to both Penaeus semisulcatus and Litopenaeus vannamei.There was a high prevalence of this bacterium in samples of different shrimp species in both seasons and both cities, which indicates a high risk of food poisoning of Pseudomonas aeruginosa due to improper consumption of shrimp. Cold chain observance during storage, transportation and sell of shrimp will be very effective in controlling this problem. Manuscript profile -
Open Access Article
25 - Evaluation of the effect of cold plasma against Pseudomonas aeruginosa inoculated in rose water
Zeinab Rahmani Fatemeh Mohamadi Reza Sharafati ChaleshtoriCold plasma is a novel non-thermal food processing technology that uses reactive medium and energetic photons and particles, to inactivate contaminating microbes on various foods. The aim of this study was to investigate the effect of atmospheric pressure cold plasma on MoreCold plasma is a novel non-thermal food processing technology that uses reactive medium and energetic photons and particles, to inactivate contaminating microbes on various foods. The aim of this study was to investigate the effect of atmospheric pressure cold plasma on Pseudomonas aeruginosa in vitro and rose water. In this experimental study, P. aeruginosa was exposed to 15 kHz plasma for 10, 30, 50 and 70 seconds. The effect of plasma on rose water inoculated with the bacterium at a concentration of 105 CFU / mL during storage for 7 days at room temperature was also investigated. The results showed that the highest non-growth halo diameter in P. aeruginosa was 31 mm in 70 seconds treatment. In addition, the plasma had an inhibitory effect on growth of P. aeruginosa in rose water during storage. Therefore, the use of plasma as a non-thermal and microbial reducing method in the rose industry is recommended. Manuscript profile -
Open Access Article
26 - Comparison of PCR and culture for diagnosis Pseudomonas aeruginosa and Escherichia coli in packed Mineral water & drinking water
Mahvash Pooyan Abbas Doosti Hamidreza Kabiri Fatemeh DreesAbstract The aim of the present study was to examine the presence of P. aeruginosa and E. coli in packed mineral and drinking water by using Polymerase Chain Reaction and microbial culture methods. In this study, 142 packed water samples were collected from active produ MoreAbstract The aim of the present study was to examine the presence of P. aeruginosa and E. coli in packed mineral and drinking water by using Polymerase Chain Reaction and microbial culture methods. In this study, 142 packed water samples were collected from active productive factories in various Provinces. Isolating and diagnosing the bacteria P. aeruginosa and E. coli were done by microbial culture and Polymerase Chain Reaction and the results were compared. E. coli contamination in PCR method, were evaluated as positive in 12 samples (14.6%) of mineral water, while all the samples in the microbial culture were negative (p=0.000). The results of study the contamination to E. coli in drinking water samples were negative based on PCR and microbial culture methods. Contamination of Pseudomonas aeruginosa was positive in 10 samples (12.1%) and 7 samples (8.5%) based on PCR and microbial culture methods, respectively (p=0.176). This value was 2 (3.3%) and 0 in drinking water by PCR and culture method, respectively (p=0.496). The results of this study indicates that Polymerase Chain Reaction can be high sensitive, specific, fast and easy method for identification. Manuscript profile -
Open Access Article
27 - گلهای رز ایرانی، یک ضد باکتری طبیعی علیه عامل اصلی عفونت سوختگی پوست، سودوموناس آئروژینوزا
مائده رزقی سید رضا حسینی دوست ژینوس عسگرپناهمقدمه و هدف : رز ایرانی (Rosa foetida) متعلق به تیره گل سرخ می باشد و یکی از گونه های بومی ایرانی به حساب می آید که تاکنون اثرات بیولوژیک گل های آن بررسی نگردیده است. از آنجا که گل های این گیاه در طب سنتی ایران به عنوان مرهم جهت بهبود سوختگی های عفونی پوست به کار می رود Moreمقدمه و هدف : رز ایرانی (Rosa foetida) متعلق به تیره گل سرخ می باشد و یکی از گونه های بومی ایرانی به حساب می آید که تاکنون اثرات بیولوژیک گل های آن بررسی نگردیده است. از آنجا که گل های این گیاه در طب سنتی ایران به عنوان مرهم جهت بهبود سوختگی های عفونی پوست به کار می رود، بر آن شدیم تا برای اولین بار اثر ضد میکروبی عصاره های مختلف گل ها را علیه عامل اصلی عفونت های سوختگی، سودوموناس آئروژینوزا بررسی نماییم. روش تحقیق: بررسی اثر ضد میکروب عصاره ها و تعیین حداقل غلظت مهار رشد باکتری به ترتیب با استفاده از روش چاهک پلیت و روش رقت سازی متوالی در میکروپلیت انجام شد. نتایچ و بحث: تمام عصاره های حاصل از گل ها دارای اثر مهاری بر رشد باکتری بودند که از بین آنها عصاره های آبی و متانولی دارای قوی ترین اثر را نشان دادند. قطر هاله عدم رشد (با غلظت 125 میلی گرم/میلی لیتر) و حداقل غلظت مهار رشد باکتری برای هر دو عصاره فوق نسبتا با اثر داروی استاندارد، ایمیپنم/سیلاستاتین (8/8 میکروگرم/میلی لیتر) یکسان بود. توصیه کاربردی / صنعتی: نتایج مشاهده شده نشان می دهد که گل های رز ایرانی دارای ترکیبات موثره با اثر ضد باکتری علیه سوش های استاندارد و پاتوژن سودوموناس آئروژینوزا جدا شده از پنج بیمار مبتلا به عفونت سوختگی پوست است و کاربرد سنتی این گیاه را در عفونت های سوختگی پوست توجیه می نماید. Manuscript profile -
Open Access Article
28 - ررسی ترکیبات شیمیایی و تاثیرات عصاره گیاهان دارویی خارمریم (Silybum marianum) و رازیانه (Foeniculum vulgare) بر برخی عوامل عفونتهای بیمارستانی و رده سلول سرطانی کبد (HepG2) به روش MTT
گلثوم صمدی اندزاگی هاشم یعقوبی مسعود فردینBackground & Aim: Increasing of drug resistances and more attention to the hazards of side effects of synthetic drugs has caused extensive researches on medicinal plant resources. The aim of this study was to determination of the chemical composition of native Silyb MoreBackground & Aim: Increasing of drug resistances and more attention to the hazards of side effects of synthetic drugs has caused extensive researches on medicinal plant resources. The aim of this study was to determination of the chemical composition of native Silybum marianum and Foeniculum vulgare extractions in Ardabil, Iran; and investigation of their anticancer effect on liver cancer cell line HepG2 and antimicrobial effects on Staphylococcusaureus and Pseudomonas aeruginosa to propose a solution to control or treatment. Experimental: The chemical compositions of extractions performed through Soxhlet were analyzed by GC/MS system. The anticancer effect was assayed by MTT method and their antimicrobial activities were tested using disc-diffusion assay as well as MIC and MBC values estimated according to the microdilution method. Results: The analysis of S. marianum extract showed the presence of 29 compounds, mostly (2.63%) Nonane, (4.68%) Camphor, (2.84%) Borneol, (3.65%) Trans-anethole, (8.78%) Viridiflorol, (34.26%) α–Selinene, (2.34%) Longiborn-9-ene, (2.52%) amorphane-B, (5.00%) Erucylamide, (10.15%) o-Terphenyl and (46.8%) α-androst-8-ene;F. vulgare extract contained 14 compounds, mainly (5.53%) Fenchone, (5.53%) α- Thujone, (2.84%) Nonane, (79.10%) trans-Anethol and (2.51%) Hentriacontane. The effects of F. vulgare extract on Staphylococcus and S. marianum extract on Pseudomonas were remarkable. In the combined design, there was no clear effect. MIC and MBC values confirmed the results of the sensitivity test. The cytotoxic effects of extractions on liver cancer cells were the optimum in 25μg/ml after 48 hours. Recommended applications/industries: According to the results, these compounds are able to replace by chemical preservatives in food and drugs industries. Manuscript profile -
Open Access Article
29 - Investigation of the frequency of exoA and exoT genes in clinical isolates of Pseudomonas aeruginosa and antibiotic resistance of strains
Shadi Mahboubi Somayeh Ataei-e JalisehPseudomonas aeruginosa is a gram-negative bacillus and an opportunistic pathogen. This bacterium is the third most common cause of nosocomial infections. Exoenzymes T, S, U, Y are secreted by the type III secretion system, which is one of the important pathogenic factor MorePseudomonas aeruginosa is a gram-negative bacillus and an opportunistic pathogen. This bacterium is the third most common cause of nosocomial infections. Exoenzymes T, S, U, Y are secreted by the type III secretion system, which is one of the important pathogenic factors of this bacterium, and exotoxin A is secreted by the type II secretion system. In this study, the frequency of exoA and exoT genes in clinical strains of Pseudomonas aeruginosa and the antibiotic resistance of the strains were investigated. 29 strains of P. aeruginosa bacteria were identified from patients admitted to Razi Hospital in Rasht, using common microbiology methods and were examined along with the standard strain of ATCC27853. The resistance of these strains to 6 types of antibiotics was determined using disk diffusion method. Then DNA extraction and PCR was performed to determine the frequency of exoA and exoT genes. In this research, the highest rate of antibiotic resistance was observed for Imipenem(86.75). The results obtained from PCR showed a frequency of 37.93%for exoA and 68.96% for exoT. Manuscript profile -
Open Access Article
30 - Investigating the antibacterial effects of polyvinyl alcohol/polyvinyl pyrrolidone composite nanofiber mat containing clindamycin
sara Yousefi mogadam Hakimeh Ziyadi Malak Hekmati Fatemeh sadat seyediDue to the expansion and importance of nano fibers in the field of pharmaceutical sciences and the ability to use them as skin patches, in this study, nanofibers carrying clindamycin was obtained from adding pure clindamycin to poly(vinyl alcohol) and poly(vinyl pyrroli MoreDue to the expansion and importance of nano fibers in the field of pharmaceutical sciences and the ability to use them as skin patches, in this study, nanofibers carrying clindamycin was obtained from adding pure clindamycin to poly(vinyl alcohol) and poly(vinyl pyrrolidone) polymers fallowed by electrospinning of polymeric solution. The obtained nanofibers were analyzed by Fourier transform infrared spectroscopy (FTIR), atomic force microscope (AFM), scanning electron microscope (SEM), Energy dispersive X-ray spectroscopy (EDS), Element Mapping (EMPA), and contact angle analysis. The nature of the bond between clindamycin drug and poly(vinyl alcohol)/poly(vinyl pyrrolidone) substrate was investigated using the quantum theory of atoms in molecules (QTAIM). Antibacterial effects of non-electrospun polymer solution and obtained nanofibers mats were investigated on standard strains of Pseudomonas aeruginosa, Acinetobacter and Staphylococcus aureus bacteria. According to the obtained results, Pseudomonas aeruginosa bacteria was resistant to non-electrospun polymer solution and clindamycin nanofibrous mats. Acinetobacter bacteria was resistant to polymer solution, but clindamycin nanofiber had moderate effect on it. Clindamycin nanofibers mats and non-electrospun polymer solution were very effective against Staphylococcus aureus bacteria. Therefore, clindamycin nanofibrous mats can be used as transdermal patches to treat infections caused by Staphylococcus aureus and Acinetobacter bacteria. In addition, the polymer solution can be useful as a medicinal solution in the transdermal treatment of Staphylococcus aureus bacterial infections. Manuscript profile -
Open Access Article
31 - Biodesulphurization of dibenzothiophene as a sulphur Model compound in heavy fuel oil by supported bacterial strain on polyethylene
Babak Ghorbani Barnaji Soroor Sadeghi farhad salimiA new biodesulfurization method has been considered using Pseudomonas aeruginosa supported on polyethylene (PE) for biodesulfurization (BDS) of dibenzothiophene (DBT) as heavy fuel oil sulphur compound model. The obtained results according to Spectrophotometric analysis MoreA new biodesulfurization method has been considered using Pseudomonas aeruginosa supported on polyethylene (PE) for biodesulfurization (BDS) of dibenzothiophene (DBT) as heavy fuel oil sulphur compound model. The obtained results according to Spectrophotometric analysis at 325 nm showed that 90.54 % of DBT at the primary concentration about 5 (mg.L-1), pH=7, biocatalyst dosage of 0.1 g, in 37 °C and after 90 min of contact time has been removed. These optimum conditions have been applied for heavy fuel oil (mazut) samples and the biodegradation of their total sulphur content (TSC) has been investigated by X- ray fluorescence spectrometer (XRF). The obtained results revealed that 33.075 % of total sulphur content from mazut sample has been removed. Kinetic study predicted the chemisorption process as the rate determining step, as it followed the pseudo-second-order rate equation. The data for DBT adsorption on biocatalyst fitted to the Freundlich isotherm model. Morphology and surface functional groups of the biocatalyst have been investigated by SEM and FT-IR, respectively. Manuscript profile -
Open Access Article
32 - Biosynthesis of ZnO nanoparticles using green tea extract and determination of its antibiofilm effect on Pseudomonas aeruginosa strains isolated from wound infection
Behrooz Shojaee Sadi Shima Chehreii Mahla EsmaeiliBackground & Objectives: Owing to the antimicrobial activity of ZnO nanoparticles without causing resistance, such substances could be considered as an appropriate alternative to prevent bacterial biofilm formation. The aim of this study was to the biosynthesis of Z MoreBackground & Objectives: Owing to the antimicrobial activity of ZnO nanoparticles without causing resistance, such substances could be considered as an appropriate alternative to prevent bacterial biofilm formation. The aim of this study was to the biosynthesis of ZnO nanoparticles using green tea extract and determination of its effect on biofilm formation in Pseudomonas aeruginosa isolates separated from wound infection. Materials & Methods: In this cross-sectional study, biosynthetic nanoparticles were evaluated by X-ray diffraction, energy dispersive X-ray analysis, scanning electron microscopy and transmission electron microscopy. Determination of antimicrobial activity and minimum inhibitory concentration of nanoparticles were done by micro broth dilution method. The antibiofilm activity was investigated using biofilm formation by O'Toole 2011 method. Results: The biosynthesis of nanoparticles was confirmed by analysis. The size of the nanoparticles was determined in the range of 10 to 90 with an average of less than 40 nm. The nanoparticles had anti-microbial activities in concentrations of 250, 500 and 1000 ug/mL and minimum inhibitory concentration of 500 ug/mL was reported. The antimicrobial and antibiofilm effects of the nanoparticles rose with increasing the concentrations. Conclusion: The biosynthesis of nanoparticles with the extract has a variety of benefits such as simplicity, good stability, without energy consumption, less time-consuming, non-toxic wastes, economical efficiency, and large scale synthesis capability. According to the antimicrobial and antibiofilm properties, the use of these nanoparticles as coatings in medical equipment and food industries is recommended. Manuscript profile -
Open Access Article
33 - Antibacterial and antibiofilm activity of bismuth oxide nanoparticles produced by Bacillus subtilis against clinical Pseudomonas aeruginosa isolated from wound infections
Leila Firouzi Dalvand Farzaneh Hosseini Shahram Moradi Dehaghi Elham Siasi TorbatiBackground & Objectives: The biological method of nanoparticles synthesis by microorganisms has a special place due to its high environmental compatibility and reduced energy consumption and costs. The aim of this study was to determine the effect of bismuth oxide n MoreBackground & Objectives: The biological method of nanoparticles synthesis by microorganisms has a special place due to its high environmental compatibility and reduced energy consumption and costs. The aim of this study was to determine the effect of bismuth oxide nanoparticles produced by green methods on bacteria causing hospital infections. Materials & Methods: In this study, 160 samples were collected from burn wound infections in patients hospitalized in Motahari Burn Rescue Hospital. Resistant strains were identified phenotypically and genotypically. Synthesis of bismuth oxide nanoparticles by Bacillus subtilis wild type strain. The synthesis of bismuth oxide nanoparticles was confirmed by infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. Finally, the antibacterial activity of nanoparticles against isolated strains was investigated with the standard disk diffusion test. Results: Biosynthesis of nanoparticles showed an average size of 44 nm using Bacillus subtilis. All 44% (71 strains) of Pseudomonas aeruginosa formed a biofilm. Bismuth oxide nanoparticles at a concentration of 2000 ppm were inhibited 5% of Pseudomonas aeruginosa. Conclusion: By increasing the concentration of bismuth oxide nanoparticles, its inhibitory effect increased. The results showed that there is a significant difference between the groups exposed nanoparticle and ciprofloxacin. Manuscript profile -
Open Access Article
34 - Quinolone resistance associated with efllux pumps mexAB-oprM in clinical isolates of Pseudomonas aeruginosa
Mitra Salehi Mina Hekmatdoost Farzaneh HosseiniBackground and Objectives: Pseudomonas aeruginosa is one of the most common antibiotic resistant bacteria and, therefore, treatments of Pseudomonad-caused infections are complicated. MexAB-OprM pump exports many antimicrobial compounds regardless of their structural and MoreBackground and Objectives: Pseudomonas aeruginosa is one of the most common antibiotic resistant bacteria and, therefore, treatments of Pseudomonad-caused infections are complicated. MexAB-OprM pump exports many antimicrobial compounds regardless of their structural and functional similarities. The mexB gene encodes a proton-drug antiporter in MexAB-OprM pump. The present study was aimed to evaluate and detect the quinolone resistance associated with efllux pumps mexAB-oprM in P. aeruginosa isolated from clinical specimens. Materials and Methods: This cross-sectional study was performed on 104 P. aeruginosa isolated from patients hospitalized in Trauma and Burn Intensive Care Unit (TBICU). The Genera and strains were identified primarily based on biochemical tests. The evaluation of antibiotic resistance pattern was carried out using disk diffusion method for 11 common antibiotics and microdilution broth method for 4 antibiotics. The presence of mexB gene was investigated using PCR method. Results: According to the results, the most antibiotic resistance pattern were seen through treatment with nalidixic acid (86.54%), ceftazidime (82.2%) and ofloxacin (81.78%). Furthermore, the minimum antibiotic resistance were observed through treatment with imipenem (40.91%), piperacillin (44.9%) and tetracycline (48.03%). Based on MIC, the highest and lowest antibiotic sensitivity was recorded for tetracycline and ceftriaxone, respectively. Based on the PCR method, 27% of the clinical isolates harbor the mexAB-oprM operon. Conclusion: Based on the results, there is a significant association between presence of mexB gene and mexAB-oprM pump and antibiotic resistance in P. aeruginosa. Regarding the importance of antibiotic resistance, the study of other efllux pumps, comparison of antibiotic resistance profile and the relationship between efllux pumps and clinical origin of the strains are recommended. Manuscript profile -
Open Access Article
35 - Study on oprD mutation and imipenem resistance in Pseudomonas aeruginosa isolates in Gilan province
Hossein Motahhary Tashi Najmeh RanjiBackground & Objectives: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, and one of the mortality causes of nosocomial infections, specifically in patients with severe burns. One of the drug resistance mechanisms in P. aeruginosa is mutation or dow MoreBackground & Objectives: Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen, and one of the mortality causes of nosocomial infections, specifically in patients with severe burns. One of the drug resistance mechanisms in P. aeruginosa is mutation or down-regulation of oprD gene. In this study, we investigated oprD mutations in imipenem-resistant P. aeruginosa isolates in Gilan province. Materials & Methods: In this sectional study, 45 P. aeruginosa strains were isolated from different clinical samples of Rasht and Lahijan hospitals and laboratories. The antibiotic resistance and susceptibility of the strains were determined by Kirby Bauer and minimum inhibitory concentration (MIC) methods. Then, PCR and sequencing were performed to evaluate oprD mutation in imipenem-resistant isolates. Results: 44.4% isolates were imipenem- resistant. The highest resistance was shown to 512 µg/ml of imipenem. Sequencing analysis showed that 5 isolates have T103S and K115T missense mutations in the oprD gene. Furthermore, silencing mutations including L92L were detected in 7 samples, S100S in 7 samples, P116P in 5 samples, and G151G in 3 samples. Conclusion: Since oprD plays, a major role in imipenem entry to the cell, oprD mutation can be the cause of imipenem resistance in P. aeruginosa isolates. Furthermore, the oprD mutation might have changed the affinity to imipenem in these isolates. Manuscript profile -
Open Access Article
36 - Genotyping of Pseudomonas aeroginusa isolated from hospital infections
Gholamreza Banisharif Hassan MomtazBackground & Objectives: Pseudomonas aeruginosa is a common opportunist pathogen in hospitals and the etiologic agent of the majority of infections in human beings. This study aimed to genotyping of P. aeruginosa isolated from hospital MoreBackground & Objectives: Pseudomonas aeruginosa is a common opportunist pathogen in hospitals and the etiologic agent of the majority of infections in human beings. This study aimed to genotyping of P. aeruginosa isolated from hospital infections. Materials & Methods: This cross-sectional study was performed on 18 isolates of P. aeruginosa isolated from hospitalized patients in Shahrekord hospitals. We applied three different methods, RAPD-PCR, Rep-PCR and ERIC-PCR for genotyping of the isolates. Results: Based on RAPD-PCR method, overall 86 different bands of DNA with a range of 300 to 1000 bps were obtained from the under study isolates and among them, 74 bands were polymorphic. Analysis of P. aeruginosa isolates based on ERIC-PCR produced 98 bands with a range of 150-8000 bps. Overall 16 genomic profile with 30 to 86% and for a few strains, 100% similarities were produced based on Rep-PCR. Conclusion: Overall, all isolates showed polymorphic band patterns and no monochromic band was observed for the isolates. The presence of polymorphic band patterns in these techniques shows high rates of polymorphism in the genome of P. aeruginosa. Furthermore, the techniques used in this study are reliable approaches for genotyping of P. aeruginosa. Manuscript profile -
Open Access Article
37 - Assessment of point mutations associated with ciprofloxacin resistance in Pseudomonas aeruginosa isolates in Guilan province
Najmeh Ranji Fatemeh Asadi Rahmani Seyedeh Shideh PourKhaliliBackground & Objectives: Pseudomonas aeruginosa is an opportunistic pathogen especially in immunocompromised patients. Drug resistance in P. aeruginosa is caused by different mechanisms such as mutations in topoisomerases subunits and negative regulators of efflux p MoreBackground & Objectives: Pseudomonas aeruginosa is an opportunistic pathogen especially in immunocompromised patients. Drug resistance in P. aeruginosa is caused by different mechanisms such as mutations in topoisomerases subunits and negative regulators of efflux pump systems. This study was aimed to investigate mutations in gyrB, parC, and nfxB genes in ciprofloxacin-resistant P.aeruginosa isolates in Guilan province. Materials & Methods: In this cross-sectional study, 200 isolates were obtained from different clinical samples of Rasht and Lahijan hospitals and laboratories and subsequently identified by biochemical tests. Antibiotic susceptibility pattern was determined by Kirby Bauer disk diffusion susceptibility test and Minimum Inhibitory Concentration (MIC) test. PCR-sequencing was used to assess mutations in gyrB, parC, and nfxB genes in ciprofloxacin-resistant P. aeruginosa isolates. Results: Out of 69 P. aeruginosa isolates, 26 isolates were ciprofloxacin-resistant. MIC of ciprofloxacin in resistant isolates was determined between 32-1024 µg/ml. PCR-sequencing analysis revealed that some resistant isolates have missense mutations in gyrB, parC, and nfxB genes. It seems that N368S, I424L, L464I, E468D, M520L, and I524V mutations in gyrB were reported for the first time in Iran. Conclusion: It seems that reported mutations in gyrB and parC genes affect topoisomerases affinity to ciprofloxacin in resistant isolates. Furthermore, mutations in the nfxB gene may lead to overexpression of MexCD-OprJ efflux pump and ciprofloxacin resistance. Manuscript profile -
Open Access Article
38 - The effect of silybin-encapsulated micelle nanoparticles on mexY expression in ciprofloxacin-resistant isolates of Pseudomonas aeruginosa
Zahra Ahmadi Roudbaraki Najmeh Ranji Aref Mohammadipour Zahra GhasemnegadBackground & Objectives: Pseudomonas aeruginosa is a major nosocomial infection in immunocompromised patients. Silybin as an herbal drug has anti-inflammatory and anti-cancer effects and recently further studies have been focused on its antibacterial functions. The MoreBackground & Objectives: Pseudomonas aeruginosa is a major nosocomial infection in immunocompromised patients. Silybin as an herbal drug has anti-inflammatory and anti-cancer effects and recently further studies have been focused on its antibacterial functions. The aim of this study was to investigate the antibacterial effect of silybin on mexY gene expression in ciprofloxacin (CP)-resistant isolates of P. aeruginosa.Materials & Methods: In this study, six ciprofloxacin- resistant isolates of P. aeruginosa were both treated by ciprofloxacin (1/2 MIC) only as a control sample and in combination with silybin-encapsulated micelles (nanoparticles) as the test sample. MBC test was performed 24 h after culturein Mueller-Hinton agar. After RNA extraction and cDNA synthesis, mexY expression was quantitatively investigated in silybin- treated and untreated cells.Results: Our findings showed that a combined silybin-encapsulated micelles and ciprofloxacin (1/2 MIC) treatment reduces the bacterial growth up to 50% after 24h. Also Q-RT-PCR analysis revealed that silybin-encapsulated micelles decrease mexY expression.Conclusion: Our results suggest that silybin could inhibit bacterial growth by decreasing mexXY-oprM efflux pump count on the cell surface. Manuscript profile -
Open Access Article
39 - Evaluation of the synergistic effect of bacterial recombinant alginate lyase and therapeutic antibiotics on the growth of planktonic Pseudomonas aeruginosa
Hadis Tavafi Ahya Abdi- Ali Parinaz Ghadam Sara GharaviBackground & Objectives: Alginate is one of the most important virulence factors of Pseudomonas aeruginosa. This study aimed to evaluate the synergistic effect of recombinant alginate lyase and common antibiotics in the treatment of Pseudomonas&n MoreBackground & Objectives: Alginate is one of the most important virulence factors of Pseudomonas aeruginosa. This study aimed to evaluate the synergistic effect of recombinant alginate lyase and common antibiotics in the treatment of Pseudomonas infections on the growth of planktonic cells of P. aeruginosa. Materials & Methods: In this study, P. aeruginosa TAG48 was isolated and identified from the clinical sample. To purify alginate lyase and its effect on planktonic cells, the alginate lyase gene (algL) was isolated, cloned, sequenced and expressed. The resultant enzyme was purified by affinity chromatography. The ciprofloxacin, tobramycin, and cefixime were also used to test the effectiveness of these antibiotics on planktonic cells of P. aeruginosa by carrying out MIC, MBC. The synergistic effects of these antibiotics and the recombinant alginate lyase enzyme on planktonic cells were evaluated. Results: Our results indicated that the antibiotics and the enzyme have shown MIC for ciprofloxacin, tobramycin, cefixime and enzyme in the following concentrations: 4, 16,128 and 9.37 μg/ml, respectively. MBC was also calculated equal to MIC. Also, alginate lyase exhibited synergy with tobramycin and cefixime on the growth of planktonic cells of P. aeruginosa TAG48 but not with ciprofloxacin. Conclusion: Regarding the importance of alginate in the pathogenicity of P. aeruginosa, its degradation could reduce this characteristic of the bacteria. Detection of novel algL genes in bacterial communities can also lead the way for the study of alginate lyases with specific activities against alginates from pathogenic bacteria in microbial samples. Manuscript profile -
Open Access Article
40 - Isolation, screening and efficiency of Pseudomonas isolates in biofilm formation on organic and inorganic carriers and phenanthrene bioremediation
maryam Tarkashvand Amir Lakzian Amir Fotovat Mehdi MohammadyBackground & Objectives: Microbial biofilms are one of the most important microorganism’s forms of life in the environment. The aim of this study was to use native Pseudomonas aeruginosa in biofilm form to clean up phenanthrene. Material & Methods: Among 6 MoreBackground & Objectives: Microbial biofilms are one of the most important microorganism’s forms of life in the environment. The aim of this study was to use native Pseudomonas aeruginosa in biofilm form to clean up phenanthrene. Material & Methods: Among 63 Pseudomonas isolates from Iranian soil, bacterial isolate with high potency in biofilm formation and crude oil degradation was selected and genetically identified. The effect of inorganic (raw pumice, processed pumice and perlite) and organic (biochar and sugarcanes bagasse) carriers on biofilm formation was investigated. Biofilm structure was studied by scanning electron microscopy. Also, the effects of two growth form (biofilm, planktonic) on biochar carrier on laccase production and phenanthrene degradation in liquid media was investigated. Results: Purified Pseudomonas isolates were screened based on biofilm formation and crude oil degradation. B59 (Pseudomonas aeruginosa) with 56.62% showed the highest percentage of crude oil degradation. Highest and lowest CFU belonged to biochar and processed pumice with 8.8 and 2.9 respectively. Laccase activity was measured in biofilm form and planktonic form 39.8 and 33.1 Um-1 respectively. Biofilm form of Pseudomonas aeruginosa led to 97.17% phenanthrene degradation where planktonic form reached 91.37%. Conclusion: Results showed that biofilm form of Pseudomonas aeruginosa compared to planktonic form by significant increase in CFU and laccase activity result in more phenanthrene degradation in liquid media. Manuscript profile -
Open Access Article
41 - Increased levels of Receptor for Advanced Glycation Products (RAGE) gene expression by Pseudomonas aeruginosa in septicemia patients
Fatemehsadat Hosseini Ashraf Kariminik Elham NasiriBackground & Objectives: Identifying the bacteria causing the infection and interacting with the immune system is too essential. RAGE is a receptor, which recognizes several endogenous and exogenous molecules and CXCL11 participates in the induction of appropriate i MoreBackground & Objectives: Identifying the bacteria causing the infection and interacting with the immune system is too essential. RAGE is a receptor, which recognizes several endogenous and exogenous molecules and CXCL11 participates in the induction of appropriate immune responses against microbes. The aim of the study was expression of the molecules in the patients suffering from septicemia versus healthy controls. Materials and Methods: In this case-control study, the expression levels of RAGE and CXCL11 in 40 patients with septicemia and 40 healthy subjects, were evaluated using Real time-PCR technique. Diagnosis of septicemia was performed by blood culture and bacterial biochemical tests. Results: RAGE mRNA levels were significantly increased in patients infected with Pseudomonas aeruginosa compared with Escherichia coli, Staphylococcus aureus and Acinetobacter bamanii. However, no significant increase in CXCL11 expression level was observed in patients and healthy controls and also in comparison with bacteria causing infection. Conclusion: Results showed that RAGE is a critical receptor against Pseudomonas aeruginosa during septicemia, Therefore, methods to reduce the expression of RAGE molecules can play a practical role in cleansing the blood from this bacterium. Manuscript profile -
Open Access Article
42 - Determination of antibiotic susceptibility pattern of Pseudomonas aeruginosa and the prevalence of bla OXA-23 gene in isolates from a Hospital in North Khorasan
Mohadeseh Radmehr majid moghbeliBackground & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strai MoreBackground & Objectives: Pseudomonas aeruginosa is one of the most important pathogens of opportunistic infections, which is considered as one of the main causes of nosocomial infections. The aim of this study was to isolate pseudomonas aeruginosa and identify strains with bla OXA-23 gene using polymerase chain reaction (PCR) technique. Materials & Methods: In this study, 70 isolates of pseudomonas aeruginosa from various infections including lung, urine, blood, wound and sputum were collected in north khorasan hospital, after phenotypic and genotypic confirmation of the isolates, antibiotic discs of doripenem and imipenem (Rosco), meropenem and ciprofloxacin, colistin and amikacin (Mast) were used to perform antibiotic susceptibility testing of bacteria by disk diffusion method. Results: Isolates showed 32.8%, resistance to amikacin , 47.1% todoripenem, 37.1% to imipenem, 40% to ciprofloxacin, and 10% to colistin. The highest resistance of the strains was related to meropenem 61.4% and the lowest was colistin with 10% resistance. Conclusion: In this study, the resistance of pseudomonas aeruginosa to the antibiotic meropenem was 61.4% and compared to other studies, including in ethiopia, showed a higher level of antibiotic resistance, this level of carbapenem resistance indicates an increase in resistance of pseudomonas aeruginosa strains to carbapenems. Manuscript profile -
Open Access Article
43 - Molecular Identification of Native Pseudomonas Strains with Polyethylene Degradation Ability from Soil
Hanieh Shahreza Abbas Akhavan Sepahi farzaneh hosseini Ramezan Ali KhavarinejadBackground & Objectives: Nowadays, high consumption plastics such as polyethylene are recognized as one of the major environmental pollutants by the World Environment Organization. The aim of this study was to isolate MoreBackground & Objectives: Nowadays, high consumption plastics such as polyethylene are recognized as one of the major environmental pollutants by the World Environment Organization. The aim of this study was to isolate and identify native Pseudomonas bacterial strains with the polyethylene packaging degradation ability. Materials & Methods: In order to conduct this study, soil samples were collected. In order to isolate the isolates with polyethylene degradation ability, two methods of direct culture and culture by pre-enrichment method were used. After culturing the bacteria in MSM medium and examining the percentage of plastic weight loss, the superior strain was selected for DNA extraction and PCR of alk-B gene. PCR results were sequenced and examined phylogenetically. Results: The results of the present study showed that the percentage of degradation of polyethylene by Pseudomonas strains was 7.2% at most and 4.5% on average. Also, all purified degrading bacteria in this study harbored alk-B gene. Conclusion: The results showed that the degradation of polyethylene materials can be significantly accelerated by using and optimizing bacteria isolated from soil. It seems that by promoting genetic methods based on the genome of bacteria, especially the strain of Pseudomonas aeruginosa, it is possible to develop methods during which all commonly used types of polyethylene are degraded in a much shorter time than normal. Manuscript profile -
Open Access Article
44 - The evaluation of the effect of gold nanoparticles on the expression of (MexA/B) efflux pump genes from the RND family in multidrug resistant Pseudomonas aeruginosa strains
Nehleh Falahati Houshang Jamali Mohammad Kargar Farshid KafilzadehBackground and purpose: Pseudomonas aeruginosa has several protein pumps with which it puts antibiotics out. This has led to the formation of multidrug resistance. The aim of this study was to investigate the effect of gold nanoparticles on the expression of mexA/B gene MoreBackground and purpose: Pseudomonas aeruginosa has several protein pumps with which it puts antibiotics out. This has led to the formation of multidrug resistance. The aim of this study was to investigate the effect of gold nanoparticles on the expression of mexA/B genes in multidrug resistant Pseudomonas aeruginosa strains. Materials and Methods: In this cross-sectional descriptive study, 74 suspected samples of Pseudomonas genus were collected and phenotypic and genotypic tests were used to screen Pseudomonas aeruginosa.Then, anibiogram test using 11 antibiotics from different classes was performed by the use of CLSI 2020 standard tables and the disk diffusion method.The frequency of mexA/B genes was evaluated by polymerase chain reaction method and after determining the minimum inhibitory concentration of gold nanoparticles, the effect of gold nanoparticles on the expression of mexA/B genes was evaluated using SYBER Green-Real Time PCR technique. Results: In this study (67.57%) 50 isolates of Pseudomonas aeruginosa were identified. The highest and lowest levels of antibiotic resistance were related to azteronam (98%) and cefpime (26%). The prevalence of mexA/B genes was respectively MexA (74%), mexB (70%) and mexA/B (58%) and also 12% lacked both genes. Gold nanoparticles showed growth inhibitory effect at MIC ≥ 50ppm and at 25 ppm concentration with ciprofloxacin, showed inhibitory effect on the expression of mexA/B genes. Conclusion: The results of this study showed that gold nanoparticles can inhibit the expression of mexA and mexB genes in multidrug-resistant Pseudomonas aeruginosa. Due to the fact that gold nanoparticle does not have toxic effects on eukaryotic cells, it can be important in the treatment process of this group of bacteria. Manuscript profile -
Open Access Article
45 - Comparing the effect of metronidazole with pyocyanin pigment extracted from Pseudomonas aeruginosa on Trichomonas vaginalis in-vitro
sara abdizadehjavazm zohreh momeni mona farhadiBackground and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important no MoreBackground and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important non-viral sexually transmitted disease in the world. The aim of this study was to evaluate the effect of pyocyanin extracted from Pseudomonas aeruginosa on Trichomonas vaginalis and PC12cell line. Materials & Methods: This study was carried out by an interventional method. First, pyocyanin was extracted from Pseudomonas aeruginosa strain RTCC1474 with the help of chloroform. The relative purity of the pigment was determined by thin-layer chromatography, spectrophotometry UV-Vis and FTIR. Its effect in different concentrations were investigated on Trichomonas vaginalis and PC12 cell line. Results: Pyocyanin at a concentration of 10,000µg/ml in 24 hours and concentrations of 5,000 and 2,500µg/ml in 48 hours caused 100% inhibition of the parasite growth. Its IC50 (Inhibitory Concentration of 50%) level in 48 hours was 17.44µg/ml and the CC50 of this pigment on the cell line was 930 µg/ml, so pyocyanin is effective against the Trichomonas vaginalis and its toxicity on the cell line is 53 times higher than of the parasite (SI=53/32). Conclusion: Considering the inhibitory effect of pyocyanin on the growth of Trichomonas vaginalis based on the results, it is possible that with further research on the extraction and purification of this pigment from various isolates of Pseudomonas aeruginosa, and with additional tests in vitro and in vivo, a more accurate judgment regarding the antiparasitic power of this pigment can be expressed. Manuscript profile -
Open Access Article
46 - The effects of Tomatidine alkaloid on biofilm formation and the exprsssion of quorum sensing associated genes in Pseudomonas aeruginosa
Hadi Ghoomdost Noori Omid Tadjrobehkar Elham MoazamianBackground and Objectives: Quorum sensing system plays an important role in regulating pathogenic propertise and biofilm formation of Pseudomonas aeruginosa. Therefore, inhibiting the quorum sensing system can be a suitable solution in the treatment of infections relate MoreBackground and Objectives: Quorum sensing system plays an important role in regulating pathogenic propertise and biofilm formation of Pseudomonas aeruginosa. Therefore, inhibiting the quorum sensing system can be a suitable solution in the treatment of infections related to Pseudomonas aeruginosa biofilm. This study was conducted to investigate the effects of sub-inhibitory concentretions of tomatidine on the expression of quorum sensing genes and biofilm formation in Pseudomonas aeruginosa. Materials and methods: The present study was a laboratory interventional study. Biofilm formation was evaluated by microtiter plate method. The RNA of all bacteria was extracted using a column RNA isolation kit and the expression of lasI, lasR, rhlI, rhlR and algD genes in both normal conditions and after treatment with tomatidine was analyzed. Antibiotic resistance was evaluated by the disk diffusion method. Results: The highest and least antibiotic resistance was detected against ticarcillin (80%) and colistin (5%), respectively. Tomatidine up to a concentration of 2 mg/ml had no inhibitory effect on Pseudomonas aeruginosa. 55% of the strains were weak biofilm producers and 45% were strong biofilm producers. The expression of all genes and biofilm formation increased after treatment with tomatidine. The lasI and rhlR genes were respectively the most and least affected genes. Among the five genes examined, only rhlR is significantly more expressed in weak biofilm producers. Conclusion: The results showed, contrary to expectation, the sub-MIC concentrations of tomatidine increase the expression of quorum sensing genes and biofilm formation in Pseudomonas aeruginosa. Manuscript profile -
Open Access Article
47 - Phenotypic and genetically evaluation of the prevalence of efflux pumps and antibiotic resistance in clinical isolates of Pseudomonas aeruginosa among burned patients admitted to Ghotbodin Shirazi Hospital
Yahya Dashtizadeh Afagh Moattari Ali akbar GorzinBackground & Objectives: Pseudomonas aeruginosa is an important cause of nosocomial infections in burnt patients. Antibiotic resistance through various mechanisms is one of challenges for treatment of these patients. The mex efflux pumps play a vital role in the dev MoreBackground & Objectives: Pseudomonas aeruginosa is an important cause of nosocomial infections in burnt patients. Antibiotic resistance through various mechanisms is one of challenges for treatment of these patients. The mex efflux pumps play a vital role in the development of multiple resistances to antimicrobial drugs. This study aimed to analyze the prevalence of genes responsible for efflux pumps mexA-B-oprM and to investigate their phenotypes in the isolates. Materials & Methods: In this cross-sectional study, 250 swabs were obtained from wounds of the patients suffering of burns levels 2 and 3 who admitted in Ghotbodin Shirazi hospital, Shiraz. Presence of P. aeruginosa isolates were confirmed by biochemical tests and PCR. Drug susceptibility, the phenotypic activity of efflux pumps and the presence of mex A, mex B genes were determined using disk diffusion method, cartwheel method and PCR methods, respectively. Results: In this study, 26.40% (66 cases) of patients with burn wounds were infected with P. aeruginosa. These bacteria were resistant to all antibiotics tested except for colistin. Totally, 66.66% of the isolates (44 cases) had an efflux pump, among them 42.92% and 87.87% of the isolates carried mexA and mexB genes, respectively. Conclusion: Our finding showed that genotypic method is very accurate and reliable than phenotypic methods for detection of efflux pumps in clinical isolates of P. aeruginosa. Due to presence of the efflux pump genes in more than 90% of the P. aeruginosa isolates, analysis of the presence of these genes is very important for suggestion of an effective treatment model for the patients with bacterial infection. Manuscript profile -
Open Access Article
48 - Immunomodulatory Effect of Propolis on IFN-γ, IL-17A and IL-23 Production in Human Peripheral Blood Mononuclear Cells Treated with Pseudomonas aeruginosa Ag
Farah Alaa Alwaeely Mohammad Abd kadhum Alsaadi Kaiser N. Madlum
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