Comparing the effect of metronidazole with pyocyanin pigment extracted from Pseudomonas aeruginosa on Trichomonas vaginalis in-vitro

Subject Areas : Protozoology

sara  abdizadehjavazm ¹ (MSc, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran)
zohreh  momeni ² (Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran)
mona  farhadi ³ (Department of Microbiology,Karaj Branch,islamic Azad University,Karaj,Iran)

Received: 2023-04-04 Accepted : 2023-08-20 Published : 2023-09-06

Keywords: metronidazole, Trichomonas vaginalis, Pseudomonas aeruginosa, Cell line, Pyocyanin pigment,

Abstract :

Background and Objectives: Pyocyanin is a blue pigment with oxidation and reduction potential and metabolically active, which has medicinal effects on eukaryotic and prokaryotic cells. Trichomonas vaginalis is the causative agent of trichomoniasis, the most important non-viral    sexually transmitted disease in the world. The aim of this study was to evaluate the effect of      pyocyanin extracted from Pseudomonas aeruginosa on Trichomonas vaginalis and PC12cell line. Materials & Methods: This study was carried out by an interventional  method. First, pyocyanin was extracted from Pseudomonas aeruginosa strain RTCC1474 with the help of chloroform. The relative purity of the pigment was determined by thin-layer chromatography, spectrophotometry UV-Vis and FTIR. Its effect in different concentrations were investigated on Trichomonas vaginalis and PC12 cell line. Results: Pyocyanin at a concentration of 10,000µg/ml in 24 hours and concentrations of 5,000 and 2,500µg/ml in 48 hours caused 100% inhibition of the parasite growth. Its IC50 (Inhibitory Concentration of 50%) level in 48 hours was 17.44µg/ml and the CC50 of this pigment on the cell line was 930 µg/ml, so pyocyanin is effective against the Trichomonas vaginalis and its toxicity on the cell line is 53 times higher than of the parasite (SI=53/32). Conclusion: Considering the inhibitory effect of pyocyanin on the growth of Trichomonas    vaginalis  based on the results, it is possible that with further research on the extraction and purification of this pigment from various isolates of Pseudomonas aeruginosa, and with additional tests in vitro and in vivo, a more accurate judgment regarding the antiparasitic power of this      pigment can be expressed.  

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