Biosynthesis of ZnO nanoparticles using green tea extract and determination of its antibiofilm effect on Pseudomonas aeruginosa strains isolated from wound infection

Subject Areas : Medical Microbiology

Behrooz  Shojaee Sadi ¹ (Instructor, Department of Biology, Faculty Of Basic Sciences, Arak Branch, Islamic Azad University, Arak, Iran.)
Shima  Chehreii ² (Assistant Professor, Department of Biology, Faculty Of Basic Sciences, Arak Branch, Islamic Azad University, Arak, Iran.)
Mahla  Esmaeili ³ (M.Sc., student, Department of Biology, Faculty Of Basic Sciences, Arak Branch, Islamic Azad University, Arak, Iran.)

Received: 2018-10-02 Accepted : 2018-10-27 Published : 2019-05-22

Keywords: Biofilm, Minimum inhibitory concentration, Green tea extract, Zinc Oxide Nanoparticles, Pseudomonas aeruginosa,

Abstract :

Background & Objectives: Owing to the antimicrobial activity of ZnO nanoparticles without causing resistance, such substances could be considered as an appropriate alternative to prevent bacterial biofilm formation. The aim of this study was to the biosynthesis of ZnO nanoparticles using green tea extract and determination of its effect on biofilm formation in Pseudomonas aeruginosa isolates separated from wound infection.   Materials & Methods: In this cross-sectional study, biosynthetic nanoparticles were evaluated by X-ray diffraction, energy dispersive X-ray analysis, scanning electron microscopy and transmission electron microscopy. Determination of antimicrobial activity and minimum inhibitory concentration of nanoparticles were done by micro broth dilution method. The antibiofilm activity was investigated using biofilm formation by O'Toole 2011 method.   Results: The biosynthesis of nanoparticles was confirmed by analysis. The size of the nanoparticles was determined in the range of 10 to 90 with an average of less than 40 nm. The nanoparticles had anti-microbial activities in concentrations of 250, 500 and 1000 ug/mL and minimum inhibitory concentration of 500 ug/mL was reported. The antimicrobial and antibiofilm effects of the nanoparticles rose with increasing the concentrations.   Conclusion: The biosynthesis of nanoparticles with the extract has a variety of benefits such as simplicity, good stability, without energy consumption, less time-consuming, non-toxic wastes, economical efficiency, and large scale synthesis capability. According to the antimicrobial and antibiofilm properties, the use of these nanoparticles as coatings in medical equipment and food industries is recommended.

References:

1. Aghaei SS, Javadi A, Sharifi Y, Morovvati A. Detection of exotoxin A, Y, T, U, S genes of
   Pseudomonas aeruginosa isolates resistant to third-generation cephalosporins in clinical
   samples of hospitalized patients in hospitals of Qom City, Iran. Qom Univ Med Sci J. 2016; 10
   (1): 48-55. [In Persian].
   2. Rajaie S, Mohammadi Sichani M, Yousefi MH. Study on the inhibitory activity of zinc oxide
   nanoparticles against Pseudomonas aeruginosa isolated from burn wounds. Qom Univ Med Sci
   J. 2015; 9(1-2): 30-37. [In Persian].
   3. Saghar Hendiani. 2013. Comparison of antibiofilm activity of silver nanoparticles and some
   antimicrobial agents against biofilm producing Acinetobacter spp. M.Sc. Basic Science Alzahra
   University. [In Persian].
   4. Tabasi M, Javadinia Sh, Masoumi Asl H, Nemati A, Azizian R, Alipour M, Tabatabaei A.
   Survey of Antibiotic Resistance and Biofilm Formation in Pseudomonas aeruginosa Isolated
   from Patients with Urinary Tract Infection. Iran J Infect Dis Trop Med. 2017; 22(77): 63-68.
   [In Persian].
   5. Ghotaslou R, Salahi Eshlaqghi B. Biofilm of Pseudomonas Aeruginosa and New Preventive
   Measures and Anti- Biofilm Agents. J Rafsanjan Univ Med Sci. 2013; 12 (9) :747-768.
   [In Persian].
   6. Rajabpour M, Arabestani M R, Yousefi mashof R, Alikhani M Y. MIC determination of
   
   Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to
   educational hospitals in Hamedan (90-91). Iran J Med Microbiol. 2013; 7(3): 18-25.
   [In Persian].
   7. Hoseinzadeh E, Samarghandi M, Alikhani M, Asgari G, Roshanaei G. Effect of zinc oxide
   (ZnO) nanoparticles on death kinetic of Gram-negative and positive bacterium. J Babol Univ
   Med Sci. 2012; 14(5): 13-19. [In Persian]
   8. Khalili H, Baghbani-arani F. Green synthesized of silver nanoparticles using Artemisia
   tschernieviana extract and evaluation of cytotoxicity effects on human colon cancer (HT29) and
   normal (HEK293) cell lines. J Ilam Univ Med Sci. 2017; 25(2): 91-100. [In Persian]
   9. obrucka R, ługaszewska J. Biosynthesis and antibacterial activity of Zno nanoparticles using
   Trifolium Pratense flower extract. Saudi J Biol Sci. 2016; 23(4): 517-523.
   10. Pourzereshki N, Naserpour Farivar T, Peymani A. Presence of alginate among multidrug
   resistant Pseudomonas aeruginosa isolated from clinical samples in Qazvin and Tehran
   hospitals. J Clin Res Paramed Sci. 2014; 3(4): 257-263. [In Persian]
   11. Emrani Sh, Zhiani R, Dafe Jafari M. The biosynthesis of silver nanoparticles using plants of
   Glycyrrhiza glabra and Mentha piperata and its antimicrobial effect on some bacteria that cause
   tooth decay. J Rafsanjan Univ Med Sci. 2018; 16(10): 953-968. [In Persian]
   12. O'Toole GA. Microtiter dish biofilm formation assay. J Vis Exp. 2011; 30(47): 1-2.
   13. Abbasvali M, Ebrahimi Kahrizsangi A, Shahriari F. Evaluation of the inhibitory effects of zinc
   oxide nanoparticles on biofilm formation of some foodborne bacterial pathogens. Iran J Med
   Microbiol. 2017; 11(5): 115-124. [In Persian]
   14. Hosseini S. 2010. The comprative evaluation of inhibitory activity of biocide SDS & Nano
   particle ZnO on biofilm formation of Candida albicans. M.Sc. Medical Science Tarbiat
   Modares University. [In Persian]
   15. Nabipour Y, Rostamzad A, Ahmadi S. The evaluation of antimicrobial properties of zink and
   silver nanoparticles on pathogenic bacteria Pseudomonas aeruginosa and Staphylococcus
   aureus. J Ilam Univ Med Sci. 2015; 23(5): 173-181. [In Persian]
   16. Rezaei A, Pourali P, Yahyaei B. Assessment of the cytotoxicity of gold nanoparticles produced
   by Bacillus cereus on hepatocyte and fibroblast cell lines. J Mol Cel Res. 2016; 29(3): 291-301.
   [In Persian]
   17. Senthilkumar SR, Sivakumar T. Green tea (Camellia sinensis) mediated synthesis of zinc oxide
   (ZnO) nanoparticles and studies on their antimicrobial activities. Int J Pharm Pharm Sci. 2014;
   6(6): 461-465.
   18 .‏Sutradhar P, Saha M. Synthesis of zinc oxide nanoparticles using tea leaf extract and its
   application for solar cell. Bull Mater Sci. 2015; 38(3): 653-657.
   19. Dhanemozhi AC, Rajeswari V, Sathyajothi S. Green synthesis of zinc oxide nanoparticle using
   green tea leaf extract for supercapacitor application. Mater Today Proc. 2017; 4(2): 660-667.
   20. Nava OJ, Luque PA, Gómez-Gutiérrez CM, Vilchis-Nestor AR, Castro-Beltrán A,
   
   Mota-González ML, Olivas A. Influence of Camellia sinensis extract on zinc oxide nanoparticle
   green synthesis. J Mol Struct. 2017; 11(34): 121-125.
   21. Padmavathy N, Vijayaraghavan R. Enhanced bioactivity of ZnO nanoparticles an antimicrobial
   study. Sci Technol Adv Mater. 2008; 9(3): 1-7.
   22. Yousef JM, Danial EN. In vitro antibacterial activity and minimum inhibitory concentration of
   zinc oxide and nano-particle zinc oxide against pathogenic strains. J Health Sci (El Monte).
   2012; 2(4): 38-42.
   23. Saadat M, Roudbar Mohammadi S, Yadegari M, Eskandari M, Khavari-nejad R. An assessment
   of antibacterial activity of ZnO nanoparticles, Catechin, and EDTA on standard strain of
   Pseudomonas aeroginusa. J Jahrom Univ Med Sci. 2012; 10(1): 13-19. [In Persian]
   24. Hoseinzadeh E, Samargandi M R, Alikhani M Y, Roshanaei G, Asgari G. Antimicrobial
   efficacy of zinc oxide nanoparticles suspension against gram negative and gram positive
   bacteria. Iran J Health Environ. 2012; 5(3): 331-342. [In Persian]
   25. Dwivedi S, Wahab R, Khan F, Mishra YK, Musarrat J, Al-Khedhairy AA. Reactive oxygen
   species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical
   determination. PloS one. 2014; 9(11): 1-9.
   26. Vincent MG, John NP, Narayanan PM, Vani C, Murugan S. In vitro study on the efficacy of
   zinc oxide and titanium dioxide nanoparticles against metallo beta-lactamase and biofilm producing Pseudomonas aeruginosa. J Appl Pharm Sci. 2014; 4(7): 41.
   27. Lee JH, Kim YG, Cho MH, Lee J. ZnO nanoparticles inhibit Pseudomonas aeruginosa biofilm
   formation and virulence factor production. Microbiol Res. 2014; 169(12): 888-896.
   28. Eshed M, Lellouche J, Gedanken A, Banin E. A Zn‐Doped CuO nanocomposite shows
   enhanced antibiofilm and antibacterial activities against Streptococcus mutans compared to
   nanosized CuO. Adv Funct Mater. 2014; 24(10): 1382-1390.
   29. Hassani Sangani M, Nakhaei Moghaddam M, Forghanifard MM. Inhibitory effect of zinc oxide
   nanoparticles on Pseudomonas aeruginosa biofilm formation. Nanomed J. 2015; 2(2): 121-128.
   30. García‐Lara B, Saucedo‐Mora MÁ, Roldán‐Sánchez J., Pérez‐Eretza B, Ramasamy M, Lee J,
   García‐Contreras R. Inhibition of quorum‐sensing‐dependent virulence factors and biofilm
   formation of clinical and environmental Pseudomonas aeruginosa strains by ZnO nanoparticles.
   Lett Appl Microbiol. 2015; 61(3): 299-305.
   31. Akhil K, Jayakumar J, Gayathri G, Khan SS. Effect of various capping agents on
   photocatalytic, antibacterial and antibiofilm activities of ZnO nanoparticles. J Photochem
   Photobiol B. 2016; 160: 32-42.