Investigating the antibacterial effects of polyvinyl alcohol/polyvinyl pyrrolidone composite nanofiber mat containing clindamycin
Subject Areas :sara Yousefi mogadam 1 , Hakimeh Ziyadi 2 , Malak Hekmati 3 , Fatemeh sadat seyedi 4
1 - Active Pharmaceutical Ingredients Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2 - Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3 - faculty of farmaceutical chemistry
4 - Department of Organic Chemistry, Faculty of Pharmaceutical Chemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Keywords: Nanofiber, Clindamycin, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter.,
Abstract :
Due to the expansion and importance of nano fibers in the field of pharmaceutical sciences and the ability to use them as skin patches, in this study, nanofibers carrying clindamycin was obtained from adding pure clindamycin to poly(vinyl alcohol) and poly(vinyl pyrrolidone) polymers fallowed by electrospinning of polymeric solution. The obtained nanofibers were analyzed by Fourier transform infrared spectroscopy (FTIR), atomic force microscope (AFM), scanning electron microscope (SEM), Energy dispersive X-ray spectroscopy (EDS), Element Mapping (EMPA), and contact angle analysis. The nature of the bond between clindamycin drug and poly(vinyl alcohol)/poly(vinyl pyrrolidone) substrate was investigated using the quantum theory of atoms in molecules (QTAIM). Antibacterial effects of non-electrospun polymer solution and obtained nanofibers mats were investigated on standard strains of Pseudomonas aeruginosa, Acinetobacter and Staphylococcus aureus bacteria. According to the obtained results, Pseudomonas aeruginosa bacteria was resistant to non-electrospun polymer solution and clindamycin nanofibrous mats. Acinetobacter bacteria was resistant to polymer solution, but clindamycin nanofiber had moderate effect on it. Clindamycin nanofibers mats and non-electrospun polymer solution were very effective against Staphylococcus aureus bacteria. Therefore, clindamycin nanofibrous mats can be used as transdermal patches to treat infections caused by Staphylococcus aureus and Acinetobacter bacteria. In addition, the polymer solution can be useful as a medicinal solution in the transdermal treatment of Staphylococcus aureus bacterial infections.
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