Synergistic effect of Froriepia subpinnata-functionalized Fe3O4 nanocomposite and ciprofloxacin on the expression of efflux pump genes in ciprofloxacin resistant isolates of Pseudomonas aeruginosa

Subject Areas : Applied Microbiology

Asal Safaei Touchaei ¹ , Kossar ramaneh ² , Mohammad Nikpassand ³ , نجمه رنجی ⁴ , Mahdi Shahriarinour ⁵

1 - Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran.
2 - Department of Biology, Faculty of Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran.
3 - Department of Chemistry, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
4 - گرو ژنتیک، دانشکد علوم، دانشگا آزاد اسالمی واحد رشت، رشت، ایران
5 - Department of Biology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran.

Received: 2024-02-28 Accepted : 2024-05-30 Published : 2024-06-09

Keywords: Ciprofloxacin, Froriepia subpinnata, Efflux pump, Magnetic nanoparticles, Pseudomonas Aeruginosa,

Abstract :

Background & Objectives: Pseudomonas aeruginosa is an opportunistic gram-negative bacterium that is a major cause of nosocomial infections such as severe burns. Froriepia subpinnata is an herbal component have antioxidant, anticancer and antibacterial properties. The aim of this study was to evaluate antibacterial effects of Froriepia subpinnata leaf extract-functionalized magnetic nanocomposite on ciprofloxacin resistant isolates of Pseudomonas aeruginosa. Materials and methods: In this study, Pseudomonas aeruginosa isolates were obtained from some hospitals and laboratories in Tehran city. After disc diffusion and MIC tests, biofilm formation assay, FIC determination and evaluation of efflux pump genes in the isolates were treated by Froriepia subpinnata leaf extract-functionalized Fe3O4 nanocomposite (1/2MIC) and ciprofloxacin (1/2MIC) (test group), and treated by ciprofloxacin (1/2MIC) (control group) were performed. Results: Synergic effects of Froriepia subpinnata leaf extract-functionalized Fe3O4 nanocomposite and ciprofloxacin was confirmed with checkerboard dilution method. Biofilm formation decreased in Froriepia subpinnata leaf extract-functionalized Fe3O4 nanocomposite (1/2MIC) and ciprofloxacin (1/2MIC) treated bacteria in compared to ciprofloxacin (1/2MIC) treated bacteria. This study showed that combination of Froriepia subpinnata leaf extract-functionalized Fe3O4 nanocomposite (1/2MIC) and ciprofloxacin (1/2MIC) led to downregulation of mexA, mexB, mexX and oprM genes in compared to ciprofloxacin (1/2MIC). Conclusion: Our results suggested that Froriepia subpinnata leaf extract-functionalized Fe3O4 nanocomposite (1/2MIC) in combination with ciprofloxacin inhibits the growth of Pseudomonas aeruginosa through decrease of biofilm formation and reducing the expression of efflux pump genes.

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