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Open Access Article
1 - The effect of Fisetin on the colorectal cancer: a review
Navideh Khodadadi Behnood Abbasi -
Open Access Article
2 - The effect of L-carnitine on colorectal cancer: A review on current evidence
Fatemeh Radkhouy Samira Soltanieh Shakiba Solgi Maedeh Ansari Behnood Abbasi -
Open Access Article
3 - A survey on the effects of citrate on canine mammary tumor cells (CF41-Mg)
Vahidi, R., Farsinejad, A., Safi, S. .Mammary tumors are among the most prevalent tumors in female dogs which according to the pathological classification have several types. These tumors have many resemblances to human tumors and can be used as a suitable model for study of human mammary tumors. Although t MoreMammary tumors are among the most prevalent tumors in female dogs which according to the pathological classification have several types. These tumors have many resemblances to human tumors and can be used as a suitable model for study of human mammary tumors. Although the antitumor effects of citrate against a variety of cancerous cells has been proven, the effect of this substance against canine mammary tumor cells has not yet been analyzed. The aim of present study was to evaluate the apoptotic and cytotoxic effects of citrate on canine mammary tumor cells(CF41-Mg) by Annexin-PI and MTT tests (a yellow tetrazole is reduced to purple formazan in living cells), respectively. MTT data showed dose dependant inhibition of citrate on proliferation of CF41-Mg cells and 50%inhibitory concentration (IC50) of this substance at a dose of26mM. In addition, percentage of apoptotic cells significantly increased after 48 hours’ treatment with IC50 of citrate (P<0/001). According to the obtained results, it seems that citrate can be used as a suitable adjuvant therapy alongside other cancer treatment substances. Manuscript profile -
Open Access Article
4 - Evaluation of the effects of celecoxib on mouse lymphoma cells (EL4)
Leyla Emami Sh Safi Farhad ZakerLymphoma is one of the most common tumors in most species of the mouse. On the other hand, biologic, histopathologic and epidemiological characteristics of mouse lymphoma are similar to lymphoma in humans. Hence, tumor lymphoma in mice can be a suitable model for the bi MoreLymphoma is one of the most common tumors in most species of the mouse. On the other hand, biologic, histopathologic and epidemiological characteristics of mouse lymphoma are similar to lymphoma in humans. Hence, tumor lymphoma in mice can be a suitable model for the biological study of human cancers. The objective of the present study was to evaluate the apoptotic and cytotoxic effects of celecoxib on mouse lymphoma tumor cells (EL4) using Annexin/PI and MTT assays. The results of MTT showed a dose and time-dependent inhibition of EL4 cells by celecoxib and 50% inhibition of cell survival at 20 μm of celecoxib. In addition, the percentage of cells with apoptosis increased after 48 hours exposure to IC50 concentration (50% survival of the exposed cells) of celecoxib. Based on the obtained results, it seems that celecoxib can be used as an adjunct therapy along with other compounds used to treat lymphoma in mice and probably in human medicine. Manuscript profile -
Open Access Article
5 - Anticancer effect of digitalis nervosa hydroalcoholic extract on cervical cancer cell line (Hela)
Nazila Ahmadzadeh Nstran Asghari Moghadam Zahra KeshtmandObjective: Today, with the high prevalence of cancer, the need for drugs with fewer side effects and better therapeutic effects has been noticed by researchers, so that a large percentage of anti-cancer compounds are prepared from natural sources. The purpose of this st MoreObjective: Today, with the high prevalence of cancer, the need for drugs with fewer side effects and better therapeutic effects has been noticed by researchers, so that a large percentage of anti-cancer compounds are prepared from natural sources. The purpose of this study is to investigate the anticancer effects of digitalis nervosa hydroalcoholic extract on uterine cancer cell line (Hela).Materials and methods: In this experimental study, the hydroalcoholic extract of the marigold plant was prepared. Then, Hela cancer cell line was treated with different concentrations of the extract (3.125, 6.25, 12.5, 25, 50 and 100 μg/ml) for 24 hours and the cytotoxicity of the extract was evaluated by MTT method. Finally, the expression level of Bax, Bcl2, P53 and CDH1 genes at 50% lethality concentration was investigated by Time-Real PCR method. Data were analyzed using SPSS software and one-way variance test.Findings: According to the results of the MTT test, the hydroalcoholic extract of foxglove has a dose-dependent cytotoxic effect against the cell line, and the concentration of 50% lethality was determined to be 24.67 micrograms per milliliter. Also, at the concentration (24.67 μg/ml), the expression of apoptotic genes Bax, CDH1 and P53 increased and the expression of Bcl2 gene decreased compared to the reference gene.Conclusion: Thimble flower plant extract has cytotoxicity and apoptosis induction effect on Hela cancer cells. Manuscript profile -
Open Access Article
6 - Examining the anticancer properties of HL-7 peptide through changes in Fas, caspase 8 and caspase 3 gene expression
Zahra Setayesh-Mehr Hosein Kamaladini Mohammad HajitabarObjective: Cervical cancer is an important cause of female mortality worldwide. The results obtained so far indicate the involvement of bioactive peptides in cancer treatment. In this regard, the aim of the present study is to investigate the anticancer properties of HL MoreObjective: Cervical cancer is an important cause of female mortality worldwide. The results obtained so far indicate the involvement of bioactive peptides in cancer treatment. In this regard, the aim of the present study is to investigate the anticancer properties of HL-7 peptide through changes in Fas, caspase 8 and caspase 3 geneexpression.Materials and methods: In this study, the viability of cervical cancer cells treated with HL-7 peptide at two concentrations of 45 and 60 μM at two times of 12 and 24 hours was investigated using MTT assay. Also, the expression of Fas, caspase 8 and caspase 3 genes was measured at mRNA and protein levels, respectively, using Real-Time PCR and Western Blot methods. Finally, the activity level of caspase 3 and caspase 8 was checked through ELISA test.Findings: The results showed that the viability of cancer cells treated with HL-7 peptide decreased in a dose- and time-dependent manner (p<0.05). By increasing the concentration of HL-7 peptide from 45 to 60 μM, significantly, the expression of Fas, caspase 8 and 3 genes increased in mRNA and protein levels (p<0.05). The results of the ELISA test showed that the activity of caspase 8 and caspase 3 significantly increased in Hela cancer cells treated with HL-7 peptide compared to untreated cancer cells (p<0.05).Conclusion: It seems that HL-7 peptide is able to eliminate cervical cancer cells by regulating the expression of genes involved in the extrinsic apoptosis signaling pathway. At the same time, more studies on molecular targets are suggested to confirm the anticancer properties of HL-7 peptide. Manuscript profile -
Open Access Article
7 - Comparison of the effect of exosomes derived from Sertoli cells with vitamin C on damage induced by electromagnetic field (50 Hz) in spermatogonial stem cells
Farzaneh Salek Javad Baharara Khadijeh Nejad Shahrokhabadi Elaheh AminiIntroduction & Objective: Spermatogonial stem cells (SSCs) as adult stem cells are crucial for spermatogenesis. Electromagnetic fields (EMF) leads to biological activity disruption of these cells and spermatogenesis. Antioxidants like vitamin C can reduce the damage MoreIntroduction & Objective: Spermatogonial stem cells (SSCs) as adult stem cells are crucial for spermatogenesis. Electromagnetic fields (EMF) leads to biological activity disruption of these cells and spermatogenesis. Antioxidants like vitamin C can reduce the damage caused by EMF through oxidative stress reduction. Recent studies also reported the key role of Sertoli cell paracrine signaling in regulating the maintenance and differentiation of SSCs. Thus, we examined and compared the effect of vitamin C and exosomes derived from Sertoli cells on damage induced by EMF in SSCs. Material and Methods: SSCs and Sertoli cells were isolated from the testes of immature male mice. The alkaline phosphatase activity of SSCs was investigated. SSCs were exposed to 50 Hz EMF intensity of 2.5 mT for one hour/five days and were treated with the optimal concentration of vitamin C and various concentrations of exosome. Then the rate of viability, colonization capacity, and apoptosis of these cells were examined. Results: Our results showed the destructive effect of EMF by reducing viability, colonization rate and alteration of SSCs nuclei. Also, these results were confirmed by increasing expression level of Caspase 9 as apoptotic gene and down-regulation of SOD as antioxidant gene. The addition of vitamin C and exosomes improved the alterations induced by EMF in SSCs; however exosomes had more ameliorative effect in comparison with vitamin C on these alterations. Conclusion: These findings demonstrated the capacity and effectiveness of exosomes as a new therapeutic agent that can restore SSCs microenvironment damaged caused by EMF exposure. Manuscript profile -
Open Access Article
8 - The effect of HIIT and thyme honey on gene expression of cardiac tissue apoptotic indices and insulin resistance index in type 2 diabetic rats
bahareh behaeen Hossein َAbedntanzi mandana gholami farshad ghazalianInroduction & Objective: Type 2 diabetes is the most common endocrine disease that can cause damage and cell death or apoptosis. The aim of this study was to investigate the expression of apoptotic and anti-apoptotic gene of heart tissue and insulin resistance index MoreInroduction & Objective: Type 2 diabetes is the most common endocrine disease that can cause damage and cell death or apoptosis. The aim of this study was to investigate the expression of apoptotic and anti-apoptotic gene of heart tissue and insulin resistance index after intense intermittent exercise and consumption of thyme honey in diabetic type 2 rats. Materials and Methods: The statistical population consisted of rats. After 20 weeks of high fat diet and injection of STZ became diabetic. Rats in 4 groups: control(n=6),HIIT(n= 8),thyme honey(n= 6),HIIT-thyme honey (n=8) trained HIIT for eight weeks, five sessions per week with intense 2-minute intense alternation with 2 to 8 alternations and with 80 to 90% vo2max and one-minute rest alternation with 50 to 56% vo2max.Thyme honey was given by gavage at a rate of 3g/kg 5days a week. Glucose, insulin and expression of Bax and Bcl2 genes and their ratio was calculated. Statistical analysis was performed using two-factor analysis of variance test and determining the effect size and Bonfroni post hoc.Results: HIIT and thyme honey decreased Bax gene expression and increased Bcl2 expression in heart cells (P<0.05). HIIT and thyme honey in diabetic rats led to improved glucose and insulin levels and decreased insulin resistance index. It also decreased the expression of Bax gene and increased the expression of Bcl2 (P<0.05). Conclusion: HIIT with thyme honey led to improved glycemic profile and changes in glucose and insulin levels, as well as positive and appropriate changes in the expression of cardiac and anti-apoptotic genes. Manuscript profile -
Open Access Article
9 - The effect of endurance training and consumption of nettle extract on the gene expression of BCL-2 and TNF-α in mice with melanoma
lila mosavi barari barari asieh Abbasi Daloii hossien Abed NatanziInroduction & Objective: : Melanoma is the most serious type of skin cancer, which is made up of melanin pigment cells.The aim of this study was to evaluate the effect of endurance training and nettle consumption on BCL-2 and TNF-α gene expression in mice with MoreInroduction & Objective: : Melanoma is the most serious type of skin cancer, which is made up of melanin pigment cells.The aim of this study was to evaluate the effect of endurance training and nettle consumption on BCL-2 and TNF-α gene expression in mice with melanoma.Material and Methods: The subjects included male Wistar rats that after two weeks of adjustment were randomly divided into 4 groups including: control, exercise, extract and exercise + extract. Exercise program included 30 minute of running on a treadmill was steep and at a speed of 16 meters per minute for the first week, and every week one meter per minute was added until the eighth week it reached 22 meters per minute. One week after melanoma induction, the experimental group consumed 30 mg / kg / day of nettle ethanol extract orally for 8 weeks. RT PCR was used to measure gene expression.Results: Data analysis showed that consumption of nettle extract and endurance training significantly reduced the expression level of BCL-2 and TNF-α genes in mice with melanoma in different groups (p = 0.000, p = 0.025, respectively). The results also showed that the difference in the level of changes in BCL-2 and TNF-α gene expression between the exercise and extract group with the control group (p = 0.002, p = 0.014, respectively) was significantly reduced.Conclusion: Regular physical activity combined with consumption of nettle extract can probably reduce anti-apoptotic factors and cause apoptosis in cancer cells by reducing inflammatory factors. Manuscript profile -
Open Access Article
10 - The effect of two training methods (continuous and periodic) and ginger supplementation on the balance of apoptosis and oxidation pathway in heart tissue of breast cancer model rats
Mohammad Hossein Bargahi parvin farzanegi Farzad Feli KohikheiliIntroduction and Objective: Today, breast cancer is one of the most common malignant diseases in women and its incidence is increasing, the aim of the present research was the effect of continuous and intermittent training methods with ginger supplement on the balance o MoreIntroduction and Objective: Today, breast cancer is one of the most common malignant diseases in women and its incidence is increasing, the aim of the present research was the effect of continuous and intermittent training methods with ginger supplement on the balance of apoptosis and oxidation pathway in heart tissue of breast cancer model rats.Methodology: In this experimental research, 56 mice were randomly divided into 8 groups: 1) control, 2) cancer, 3) cancer-saline, 4) cancer-supplement, 5) cancer-periodic, 6) cancer-continuous, 7) cancer-periodic-supplement and 8) cancer-continuous-supplement group were placed. The continuous program of the first week with a speed of 15 m/min for a duration of five minutes on the treadmill, which increased the speed by 1-2 m/min every week and increased the running time by 1-2 minutes. Intermittent training includes six sets of 2.5 minutes, with four minutes of rest between each set. Results: The results showed that the induction of cancer increased the expression of BAX and MDA in the heart tissue of rats and significantly decreased the expression of BCL-2 and SOD, each of the interventions (continuous exercise, ginger and the combination of continuous and intermittent exercise with ginger supplement) The cardiac expression of BAX and MDA decreased and the expression of BCL-2 and SOD increased significantly.Conclusion: The results confirm the role of continuous and periodic exercise with ginger supplement in improving the apoptotic and oxidative indices of heart tissue in breast cancer mice, which may have a protective effect on heart tissue. Manuscript profile -
Open Access Article
11 - The interactive effect of swimming training and vitamin D supplements on Bcl-2 gene expression in heart tissue of cadmium-poisoned rats
Amir Atefat Izadi Javid Ouji -
Open Access Article
12 - The effect of eight weeks of aerobic training on some apoptotic factors of elderly men
Farzane Tleb Mohammadi bahram abedi Mojtaba KhansoozObjective: Impairment in the secretion and function of insulin in different tissues of the body is the main cause of type 2 diabetes. The purpose of this study is to investigate the effect of intense intermittent exercise and thyme honey on GLUT4 gene expression in sole MoreObjective: Impairment in the secretion and function of insulin in different tissues of the body is the main cause of type 2 diabetes. The purpose of this study is to investigate the effect of intense intermittent exercise and thyme honey on GLUT4 gene expression in soleus muscle and insulin resistance index in male obese rats with It is type 2 diabetes.Materials and Methods: In this experimental study, 36 rats (5-8 weeks old, weighing 110±10 g) were randomly divided into 4 groups of diabetes, diabetes-exercise, diabetes-supplement and diabetes-exercise-supplement. To induce type 2 diabetes, STZ was injected intraperitoneally at a dose of 25 mg/kg. Intense interval training was performed for 5 sessions per week for 8 weeks. Thyme honey in the amount of 3 g/kg was gavage in the supplement groups for 5 days a week before training. After blood sampling and tissue sampling, GLUT4 gene expression of soleus muscle and insulin resistance index were measured for data analysis using ANOVA test. Bonferroni's one-sided and post hoc tests were used at a significance level of P<0.05.Findings: Insulin changes in exercise group, supplement and supplement exercise showed a significant increase (P<0.05), insulin resistance values in the supplement group increased significantly compared to the control group (P=0.001). But the changes of this index in the training group showed a significant decrease compared to the control group, supplement and supplement training group. (p≥0.001) The level of GLUT4 has increased significantly in the training group and the training-supplement group. (P<0.05) Glucose changes in the exercise group and the exercise-supplement group were associated with a significant decrease (P<0.05).Conclusion: In addition to the findings of this research, it seems that eight weeks of intermittent exercise alone and together with the consumption of thyme honey improves insulin resistance and increases GLUT4 gene expression in diabetic samples. Manuscript profile -
Open Access Article
13 - The effect of synthetic silver nanoparticle from hydroalcoholic extract Digitalis nervosa on CDH1, Bax , Bcl2 and P53 gene expressions in cervical cancer cell line)Hela(
nazila ahmadzadeh nastran asghari moghaddam zahra keshtmandNowadays, metal nanoparticles, especially silver, due to their special physical properties and anti-cancer effects have been considered.One of the methods of synthesis of these nanoparticles is the use of plant extracts. The aim of this study was to investigate the anti MoreNowadays, metal nanoparticles, especially silver, due to their special physical properties and anti-cancer effects have been considered.One of the methods of synthesis of these nanoparticles is the use of plant extracts. The aim of this study was to investigate the anti-cancer effects of silver nanoparticles synthesized of the hydroalcoholic extract of Digitalis nervosa on the cancer cell line (Hela).In this experimental study,the synthesis of silver nanoparticles was performed by adding the hydroalcoholic extract of Digitalis nervosa to the silver nitrate solution. Physical and chemical properties of synthesized nanoparticles were determined by UV-vis, TEM, SEM, EDX and XRD methods.Viability of Hela cancer cells by MTT method with different concentrations of synthesized silver nanoparticles(3.125 to 100 mg/ml) and the expression of Bax, Bcl2, P53 and CDH1 genes were evaluated using real time PCR technique in 24 hours.Data were analyzed using GraphPad software and one-way ANOVA test.P<0.05 was considered statistically significant.The results showed that the results of cytotoxicity at different concentrations of silver nanoparticles showed that IC50 was16.66 μg /ml, respectively. Also, the expression of Bax, CDH1 and P53 apoptotic genes in concentration-(16.66μg/ml) increased and Bcl2 gene expression decreased compared to the reference gene, which showed the effects of cytotoxicity and changes in nanoparticle concentration. Silver nanoparticles synthesized from the extract of the Digitalis nervosa plant have a cytotoxic effect and possibly induce apoptosis on Hela cancer cells. Manuscript profile -
Open Access Article
14 - Effect of regular aerobic exercise on serum levels of cTnI, TNF-α and apoptosis in cardiac ischemia-reperfusion injury of the rats
یوسف DoustarThe process of restoring blood flow to ischemic heart muscle is antithetically capable of inducing cardiac damage. Cardiac troponin I (cTnI) and tumor necrosis factor alpha (TNF-α) are t MoreThe process of restoring blood flow to ischemic heart muscle is antithetically capable of inducing cardiac damage. Cardiac troponin I (cTnI) and tumor necrosis factor alpha (TNF-α) are the important biochemical parameters of cardiac tissue damage. The aim of this study was to evaluate the effects of short term and regular growing long term aerobic exercise on serum levels of cTnI and TNF-α in rats with Ischemia/Reperfusion (I/R) injury. For this purpose, forty male Wistar rats were randomly divided into four equal groups including: control, I/R, I/R with two weeks of aerobic exercise, and I/R with eight weeks of regular growing aerobic exercise groups. Aerobic exercise was performed 5 times per week on treadmill at speed of 10-25m/min for 10-30 minutes with the slope of 5 degrees. For induction of I/R injury, the left descending coronary artery was clamped for 30 minutes, thereafter blood flow was restored for 2 hours. Finally, after collection of blood samples from the retro-orbital plexus for cTnI and TNF-α measurements, all animals were euthanized. Histologic sections were created for TUNEL staining from the hearts. Regular growing long term aerobic exercise significantly (p<0.05) decreased the cTnI and TNF-α serum levels, which were increased due to I/R injury. Microscopically, the numbers of apoptotic cells were significantly (p<0.01) increased in I/R group compared to the control group. Regular growing long term aerobic exercise significantly decreased the number of apoptotic cells (p<0.05). The results showed regular growing long term aerobic exercise protects the cardiac tissue of rats from I/R injury. Conflict of interest: None declared. Manuscript profile -
Open Access Article
15 - Effects of unilateral uretral obstruction on the induction of renal apoptosis
یوسف Doustar مهرداد Neshat Garamaleki Ali Rezaie Abnormalities of cell numbers are a frequent feature of renal disorders. Programmed cell death is a key factor in the regulation of cell numbers. Apoptosis is an active form of cell death that is modulated by extra cellular lethal and survival More Abnormalities of cell numbers are a frequent feature of renal disorders. Programmed cell death is a key factor in the regulation of cell numbers. Apoptosis is an active form of cell death that is modulated by extra cellular lethal and survival signals. Intracellular survival factors also have an important role in the regulation of apoptosis. The aim of this study was to investigate apoptosis of the renal tubular epithelial cells following unilateral uretral obstruction. This study was conducted on 10 dogs allocated equally into treatment and control groups. In the treatment group, unilateral uretral obstruction was created surgically while animals in the control group were subjected to usual nutritional and caring conditions without surgery. After a week, renal tissue samples were collected from both groups. The samples were fixed in 10% buffered formalin and 4-5 micron thick sections were prepared and stained by TUNEL technique. Statistical analysis was performed using the Mann-Whitney U test. The histopathological examination of the treatment group revealed increased apoptosis of renal tubular cells. The average number of apoptotic cells in the treatment group, obtained from calculations made in five different microscopic fields was significantly higher than the control group (P<0.005). This study indicated that canine unilateral uretral obstruction as an animal model can induce apoptosis in renal tubular cells. Manuscript profile -
Open Access Article
16 - Experimental study of apoptosis in pancreas of SPF chickens following infection by H9N2 serotype of influenza virus
یوسف Doustar داریوش Mohajeri Influenza virus is the cause of cell death in animal and human tissues. Cell death can take place in the form of necrosis or apoptosis. In this study, the type of cell death in the pancreatic tissue of chickens infected by H9N2 serotype of influenza More Influenza virus is the cause of cell death in animal and human tissues. Cell death can take place in the form of necrosis or apoptosis. In this study, the type of cell death in the pancreatic tissue of chickens infected by H9N2 serotype of influenza virus (A/chicken/Iran/772/2000) is experimentally investigated. In the present study, 20 SPF chickens with the age of 3- weeks were randomly allocated to two equal groups. The first group was infected with 0.2 ml of 1:10 dilution and 107.5 EID50 titer of the virus and the second group with equal volume of normal saline solution intranasaly. After 72 hours, specimens were collected from the pancreatic tissue and 5 μm thick microscopic sections were prepared from specimens fixed in 10% buffered formalin and stained with hematoxylin and eosin. Histopathological examination of the pancreatic tissue revealed that there was significant difference in the mean number of apoptotic and necrotic cells between the control and treatment groups (p<0.005). The results of this study indicated that the H9N2 serotype of influenza virus has the potential to induce apoptosis in acinar, alpha and beta islet cells of pancreas. Manuscript profile -
Open Access Article
17 - Experimental study of apoptosis in the prostate tissue following castration
یوسف Doustar Ali Rezaie صابر Atash BenabThe Prostate gland is one of the accessory reproductive glands with important physiological functions necessary for successful reproduction. This gland depends on the presence of sex hormones including androgens for its natural function and normal growth and development MoreThe Prostate gland is one of the accessory reproductive glands with important physiological functions necessary for successful reproduction. This gland depends on the presence of sex hormones including androgens for its natural function and normal growth and development. So in the case of hyperplasia, hypertrophy or other prostatic disease the most successful and efficient method of treatment is androgenic control that in some cases is unavoidable. The purpose of this study was to investigate the effects of androgenic depletion states by means of castration on the induction of apoptosis in the epithelial glandular cells of the prostate tissue. Two groups of male dogs each containing 5 animals per group were used in this study. The dogs were under observation for 1 month to detect any possible diseases or disorders. After this period the dogs in the treatment group underwent open castration to decrease the levels of the androgenic hormones in the blood while the dogs in the control group were left intact. One week after surgery, the prostate glands of control and treatment animals were collected and used to prepare microscopic sections. The sections were evaluated following staining with TUNEL (TerminaldeoxyNucleotidyl (dUTP) transferase-mediated End Labeling) and H&E methods. The Mann – Whitney U test was used for statistical analysis. Histopathological studies in the treatment group revealed the presence of various forms of apoptotic cells in the glandular epithelium. Average number of apoptotic cells in ten microscopic fields were significantly higher in the treatment group compared with the control group (p<0.005). These results demonstrated that the type of cell death observed in the prostate gland in androgen deprivation states is Apoptosis. Manuscript profile -
Open Access Article
18 - Comparison of apoptosis in canine transmissible veneral tumor (TVT) pre and post chemotraoy with vincristine sulphate
یوسف Doustar داریوش Mohajeri رامین Kafash ElahiThe canine transmissible veneral tumor (CTVT) is a prevalent tumor in canidae. It is transmitted by coitus, forming multiple neoplastic masses on the external genitalia of both sexes within the family canidae. CTVT have an aberrant karyotype and the origin of the neopla MoreThe canine transmissible veneral tumor (CTVT) is a prevalent tumor in canidae. It is transmitted by coitus, forming multiple neoplastic masses on the external genitalia of both sexes within the family canidae. CTVT have an aberrant karyotype and the origin of the neoplastic cells is undetermined but immunophenotyping suggests that the tumor has a histocytic origin. In this study 10 dogs with canine transmissible veneral tumor were selected and received vincristine sulphate (0.025 mg/kg/b.w) chemotrapy to induce apoptosis in neoplastic cells. Biopsy specimens were collected from tumors during the growth phase, before and again after chemotherapy from the same dogs. The specimens were fixed in 10% formalin and then prepared routinely for H&E and TUNEL assays. Histopathological study of tissue section of CTVT before chemotherapy revealed sheets of uniform neoplastic cells, round to oval in shape with defined cytoplasmic border. There were a few TUNEL positive cells and mitotic figures. In tumor specimens after chemotherapy increased TUNEL positive cells and depilation of neoplastic cells in stroma of tumor were observed. Mean deference of histopathological changes and TUNEL positive cells before and after chemotherapy were significant (p<0.003). This study indicates that vincristine sulphate is capable of induction of apoptosis in neoplastic cells of CTVT. Manuscript profile -
Open Access Article
19 - Photodynamic therapy for melanoma: a multifaceted anti-cancer treatment against malignant melanoma
Neda Rajabi Mahsa Gholizadeh Sanaz Baghban Khoshechin Mohammad Amin Doustvandi Fateme Mohammadnejad Habib Tajalli Behzad Baradaran -
Open Access Article
20 - The Investigation of the Toxicity of Palladium Nanoparticles on Human Lymphocyte
maryam zivari fard majid sharifi arian shojaei Seyed Mahdi Rezayat seyyedeh elaheh mousavi Mojtaba Falahati -
Open Access Article
21 - The Investigation of the Toxicity of Palladium Nanoparticles on Human Lymphocyte
Maryam Zivari fard Majid Sharifi Arian Shojaei Seyed Mahdi Rezayat Seyyedeh Elaheh Mousavi Mojtaba Falahati -
Open Access Article
22 - The effect of eight weeks of resistance training after transplantation of stem cells derived from adipose tissue on some apoptotic and anti-apoptotic factors of heart tissue in non-alcoholic fatty liver model rats
Erfan Gholami Seyed Abdollah Hashemvarzi Seyedeh Yasaman AsadiIntroduction: Nonalcoholic fatty liver disease is one of the most common chronic hepatic diseases which may be associated with cardiovascular disease. The study was conducted with the aim of investigating the effect of eight weeks of resistance training after stem cell MoreIntroduction: Nonalcoholic fatty liver disease is one of the most common chronic hepatic diseases which may be associated with cardiovascular disease. The study was conducted with the aim of investigating the effect of eight weeks of resistance training after stem cell transplantation in fatty liver model rats. Methods: 36 rats were divided into two groups: control (6 rats) and patients with fatty liver (30 rats). The control group used standard food for 6 weeks, while the animals in the diseased groups used high-fat food to induce fatty liver disease. At the end of the sixth week, to confirm the development of fatty liver disease, blood was randomly drawn from the tails of 3 rats. Then the animals of the patient group were randomly divided into 5 groups Saline, Fatty liver, Exercise, Cell, Exercise + Cell. About 1.5 x 106 stem cells for each mouse were injected into the cell receiving groups through the tail vein. The training program consisted of 8 weeks of climbing the ladder. Results: The amounts of Bax and Bcl-2 of the heart tissue decreased and increased respectively in the treated groups compared to the patient group, but these changes were significant only in the exercise group and exercise + cells compared to the patient group. Conclusion: Non-alcoholic fatty liver disease is significantly associated with cardiac cell apoptosis. Resistance training after stem cell transplantation decreased the Bax and increased the Bcl-2 in heart tissue. Therefore, it seems that this method can be considered as a therapeutic solution. Manuscript profile -
Open Access Article
23 - آپوپتوز ناشی از اسانس دانه عناب در سلول های سرطانی کولورکتال لاین HT-29
زهرا زمانی الهام قدوسی دهنوی رضا حاجی حسینی محمد ارجمند سیما نصریBackground & Aim: In traditional medicine, Zizyphus jujuba (jujube) has been used due to its medicinal properties and various physiological functions such as antioxidant, anticancer and anti-inflammatory properties. This study was conducted to study anti-cancer and MoreBackground & Aim: In traditional medicine, Zizyphus jujuba (jujube) has been used due to its medicinal properties and various physiological functions such as antioxidant, anticancer and anti-inflammatory properties. This study was conducted to study anti-cancer and apoptotic effects of essential oil from Zizyphus jujuba seeds and to evaluate the effect of essential oils on p53, APC and KRAS genes expression in HT-29 colorectal cell line.Experimental: In this study, the essential oil of jujube seeds collected from orchards in Isfahan was prepared by Clevenger apparatus and then analyzed by GC-MS. The effects of toxicity and cell viability were determined using Trypan Blue, MTT and clonogenic methods. DNA fragmentation and apoptosis techniques were used to evaluate the mechanism of the effects of essential oil on cell death by flow cytometry. Finally, Real-time PCR was used to evaluate the expression of p53, APC and KRAS genes and their role in induction of apoptosis.Results: Essential oil of Zizyphus jujuba seeds reduced the viability of cells by concentration and time-dependent manner as compared to the control group. The essential oil also induced apoptosis by increasing gene expression p53 and suppressing gene expression KRAS.Recommended applications/industries: The present results indicated that essential oil of Zizyphus jujuba seeds induces apoptosis by targeting genes involved in colon carcinogenesis. However, further investigations on signaling pathways are needed to fully confirm the results of this study. Manuscript profile -
Open Access Article
24 - بررسی تغییرات هماتولوژیک و ایمونولوژیک ناشی از مصرف جلبک اسپیرولینا در مقایسه با داروی لوامیزول در سگ های نژاد پرشین شفرد
سام ترکان محمد جواد عکاشهBackground & Aim: Spirulina microalgae is known to has beneficial effects in the treatment of many animals diseases. Effects of Spirulina platensis microalgae, singly or in comparison with levamisole drug are not still studied in dogs. Thus, the present study was co MoreBackground & Aim: Spirulina microalgae is known to has beneficial effects in the treatment of many animals diseases. Effects of Spirulina platensis microalgae, singly or in comparison with levamisole drug are not still studied in dogs. Thus, the present study was conducted to investigate the effects of this microalgae on hematological and immunological parameters in dogs.Experimental: In this research, 24 native breed dogs were used for 30 days. The dogs were divided into three groups of eight randomly, including a control group, treatment group (received 2 g Spirulina orally) and the third group received 5­mg/kg Levamisole. At the end of mentioned period, hematocrit percent, Hb, RBC, WBC, Neutrophil, Lymphocyte, Eosinophil, monocyte, basophil levels and immunological factors including IgM, total protein and phagocytic precent were measured.Results: Hematocrit in levamisole receiving group increased significantly (P<0.05). Results showed that RBC and Hb levels in Spirulina receiving group were significantly higher than the control and levamisole groups. Alb and Neutrophil levels in Spirulina receiving group were lower than the other two groups. In addition, Eosinophil and WBC levels in control group were significantly lower than the other two groups, but monocyte and Lymphocyte levels in control group increased significantly (P<0.001). Also, on the 30th day, total protein and phagocytic percent in Spirulina receiving group were significantly higher than others groups. In addition, in Spirulina and levamisole groups a significant increase in IgM level was observed (P<0.001).Recommended applications/industries: It can be concluded that the use of Spirulina in dogs for a short time could improve hematological and immunological factors and the effects of Spirulina alga in the most factors is comparable to levamisole drug. Manuscript profile -
Open Access Article
25 - Anti-Apoptotic Role of p21 and p27 Genes during Different Stages of Corpus Luteum Progression in Bovine
H. Ali S. Hayat S. Ahmad M. Ibrahim S.A. Haider S. Ullah S. Ahmad H. Khan I. Ul Haq -
Open Access Article
26 - Studying MiR-133 influencing the process of apoptosis of Cardiomyocytes floating in blood serum of patients suffering from acute Myocardial Infarction
shiva kasiolkheir changiz ahmadizadeh hoshang hosseinzadeh mogbeliAbstract Background and goals: Myocardial Infarction is known as one of the most common disabling and threatening heart diseases resulted from irrevocable and degenerative cellular death in a part of heart muscle. After the discovery of miRNA in 1990 and the discovery o MoreAbstract Background and goals: Myocardial Infarction is known as one of the most common disabling and threatening heart diseases resulted from irrevocable and degenerative cellular death in a part of heart muscle. After the discovery of miRNA in 1990 and the discovery of more than 2500 types of miRNA, gradually the importance of these mechanism regulators and molecular signals and gene routes were identified in the processes and the cellular mechanisms, specifically in cardiovascular system. The goal of this research is Studying MiR-133 influencing the process of apoptosis of Cardiomyocytes floating in blood serum of patients suffering from acute Myocardial Infarction Methods: To achieve our goals in this case-observation study type regarding miR-133, 70 patients in Shahid Madani Hospital in Tabriz in the year 2017 were investigated by Real time PCR and the data were compared with those of the healthy persons. The statistical analyses were carried out using SPSS (version19) and a t-test method. The amounts for P>0.05 were considered meaningful. Findings: The expression levels of miR-133 among patients suffering from MI have had a considerable increase compared with control group and it was meaningful statistically. (P=0.009). Also the results showed that miR-133expression among fat and normal people did not have a meaningful difference statistically (P=0.06). Conclusion: The present study showed that the expression of miR-133among individuals suffering from MI has been greater than healthy people and it can be utilized as an identification factor and also prognosis of the patients suffering from MI. Manuscript profile -
Open Access Article
27 - Effect of vitamin C on apoptosis via measurement of caspase (1) gene expression in heart and lung of broiler chickens with pulmonary hypertension syndrome
hamed zarei Azade RashtiPulmonary hypertension syndrome with high pulmonary arterial pressure, right ventricular hypertrophy and dilation is a problem of broilers. On the other hand it is proved that apoptosis in heart failure and pulmonary hypertension increases. In this study, for the first MorePulmonary hypertension syndrome with high pulmonary arterial pressure, right ventricular hypertrophy and dilation is a problem of broilers. On the other hand it is proved that apoptosis in heart failure and pulmonary hypertension increases. In this study, for the first the effect of vitamin c on apoptosis by measuring the expression of caspase (1) in the heart and lungs of broilers with pulmonary hypertension syndrome was evaluated. T3 as a thyroid hormone was added to the ration after week 1 of rearing. Pulmonary hypertension was induced at 49 days based on RV/TV ratio index. After PCR for caspase1 and b-actin (Housekeeping) genes the density of each band were measured and were recorded as the ratio caspase1 / β-actin and this ratio were compared at different ages in witness groups (the right ventricle and lung). The amount of mRNA of the caspase 1 gene in the right ventricle at 21 and 49 days and in lung tissue at 49 days significantly reduced in the treatment group compared to the control group(p<0.05), this significant difference represents the reduction of apoptosis in the group who by receiving the hormone T3 were infected to pulmonary hypertension, and yet have been treated with vitamin C. Also, according to the results, the RV/ TV ratio improved in the treatment group. Manuscript profile -
Open Access Article
28 - Study the expression of BAX gene subsequent effect of cholestasis and treatment by neuroaid in cerebellum of male rats
afsaneh karimi anche Mehrdad Hashemi Mohamed NasehiBackground: Including liver diseases is cholestasis which is a consequence of disordered bile secretion causes an accumulation of poisonous bile in the body. If untreated, Cholestasis is causing damage to various organs. In this research was planned to investigate the e MoreBackground: Including liver diseases is cholestasis which is a consequence of disordered bile secretion causes an accumulation of poisonous bile in the body. If untreated, Cholestasis is causing damage to various organs. In this research was planned to investigate the effects of cholestasis and neuroaid (a drug which protects and repairs neurons) on the expression of Bax gene, in cerebellum of rat brain. Bax has role in apoptosis. Methods: A total number of 16 rats were divided into four groups as follows: Control group. BDL group, the rats which were just operated for bile duct ligation. BDL-neuroaid group, the rats which received both operation and neuroaid. Sham-neuroaid group, the rats which were not operated, but received the operation stress and neuroaid. Following the treatments, the rats were killed and their cerebellum were removed from their brain. RNA was extracted from the cerebellum cells, cDNA was synthesized, and Real time PCR was performed to measure the gene expression. Results: According To the results, cholestasis causes increase in the expression of Bax gene; Neuroaid causes reduces in the expression Bax gene. Conclusion: Cholestasis changes is benefit to increase internal apoptosis pathway, however, neuroaid can approximately reduce this effect as well through making reduces Bax gene and as a result reduction of apoptosis, in cerebellum of rat brain Manuscript profile -
Open Access Article
29 - Cytotoxic Effect of Girard-T Reagent-based Schiff Bases and their Dimethyltin (IV) Complexes on Human Colon Cancer Cells
elham hoveizi zynab ansariaslDespite many efforts, no definitive treatment is yet knowing for cancer. Therefore, research on new compounds that have anti-cancer properties is especially important. The aim of this study is investigation the cytotoxic effect of Girard-T reagent-based Schiff bases and MoreDespite many efforts, no definitive treatment is yet knowing for cancer. Therefore, research on new compounds that have anti-cancer properties is especially important. The aim of this study is investigation the cytotoxic effect of Girard-T reagent-based Schiff bases and their complexes on HT29 cancer cells. HT29 cells was cultured in DMEM/F12 medium containing FBS 10% and antibiotics 1% then the effects of ligands and their complexes with the concentration of 0.1, 1 and 5 mg/ml were surveyed on these cells in 1, 2, and 3 days. Growth, proliferation and morphological changes were photographed using an inverted microscope. MTT assay and DAPI staining were used to quantify cell viability. With concentrations of 0.1, 1, and 5 mg/ml of [SnMe2(L2)] (the effective component) the survival were decreased to 34, 24 and 18% after 72h(P<0.05). Encountering cells with complex caused to induce apoptosis in relation with concentration so that, the cell viability of cells treated with concentrations of 5 mg/ml (the effective concentration) of (H2L1) ،(H2L2) ،[SnMe2(L1)]،[SnMe2(L2)] was 22, 18, 29, and 22 respectivly after 72h and the highest apoptosis (82%) happened in 5mg/ml of SnMe2(L2). The synthetic components have a toxic and ant-proliferation effect on HT29 in relation with concentration. Manuscript profile -
Open Access Article
30 - Neuroprotective effect of Neurokinin 1 receptor antagonist on CA1 region of hippocampus in male Wistar rats following ischemic / reperfusion induction
Shervin Eftekhari Shabnam Movassaghi Amir Ghasemi Zahra Nadia SharifiCerebral ischemia/reperfusion (I/R) injury is a critical factor leading to a poor prognosis for ischemic stroke patients. Substance P-mediated inflammation is reported to attenuate the neuroprotective PPAR-γ. In This study, we determined the effects of aprepitant, MoreCerebral ischemia/reperfusion (I/R) injury is a critical factor leading to a poor prognosis for ischemic stroke patients. Substance P-mediated inflammation is reported to attenuate the neuroprotective PPAR-γ. In This study, we determined the effects of aprepitant, a substance P-NK1 receptor antagonist in bilateral common carotid arteries occlusion (BCCAO) induced I/R brain injury. 24 male Wistar rats were divided into4 groups ( Control- Ischemia - Vehicle and experimental). Ischemia model was induced by ligation of bilateral common carotid arteries. Aprepitant (40mg/kg) was administered twice, one hour beforethe ischemia and one hour after the reperfusion. After 72 hours, Brains were removed and prepared for Nissl staining.Data depicted that significant differences were seen in the number of viable Pyramidal cells in CA1 region between control and ischemia groups whereas there are no significant deference between experimental and control groups.It may be concluded that aprepitant can reduce post-ischemic tissue lesions, so may candidate for the treatment of I/R brain damage. Manuscript profile -
Open Access Article
31 - Comparative investigation of cytotoxicity effects of iron oxide, zinc oxide and silver nanoparticles on white blood cells and leukemic cell line HPB-ALL in Vitro
Zahra Farahani Kazem Parivar nasim HAYATI Mona FarhadiTreatment with nanoparticles has attracted the attention of many researchers. Due to their small size and easy absorption, nanoparticles can be a suitable option for treatment. In this study, a comparative study of the anticancer effect of silver nanoparticles, zinc oxi MoreTreatment with nanoparticles has attracted the attention of many researchers. Due to their small size and easy absorption, nanoparticles can be a suitable option for treatment. In this study, a comparative study of the anticancer effect of silver nanoparticles, zinc oxide and iron oxide on leukemic cell line and white blood cells of HPB-ALL was done.Human white blood cells and leukemia cell line HPB-ALL were passaged. Iron oxide, zinc oxide and silver nanoparticles with different concentrations were added to the microplates containing the cells and the cells were treated with nanoparticles for 24 hours. PI was evaluated. The results obtained for leukemic cell line HPB-ALL and white blood cells were analyzed with the effect of three nanoparticles of silver, zinc oxide and iron oxide at a significant level (P<0.05). All three nanoparticles showed cytotoxic effects in both cell lines. The cytotoxicity effects of nanoparticles were higher in leukemic cell line HPB-ALL than in white blood cells. Among these nanoparticles, silver nanoparticles had a significant cytotoxic effect in HPB-ALL leukemic cell line compared to the other two nanoparticles. Evaluation of IC50, DNA damage and apoptosis induction in HPB-All leukemic cell line by silver nanoparticles was significantly higher than other two nanoparticles. According to the above results and further investigations of the penetration mechanism of each of the nanoparticles, it can be said that silver nanoparticles have the potential for therapeutic applications. Manuscript profile -
Open Access Article
32 - Anti-cancer effect of Artemisia turcomanica extract on breast cancer cell line (MCF-7)
zahra keshtmand Seyede narges naimi , Ardeshir HesampourThe prevalence of cancer and the side effects of treatment methods have provided a growing interest in the use of plants as a promising source of treatment.The use of medicinal plants in traditional medicine has been important for a long time and many of these medicinal MoreThe prevalence of cancer and the side effects of treatment methods have provided a growing interest in the use of plants as a promising source of treatment.The use of medicinal plants in traditional medicine has been important for a long time and many of these medicinal plants have antioxidant and anticancer effects. The purpose of this study is to investigate the anticancer effects of Artemisia turcomanica extract on breast cancer cell line (MCF-7)and its effect on Bcl2 and Bax gene expression.In this experimental study, the effect of the hydroalcoholic extract of Artemisia turcomanica on viability and expression of Bax and Bcl2 genes in MCF-7 breast cancer cells was examined.For this purpose,MCF-7breast cancer cells were cultured and treated with the hydroalcoholic extract of Artemisia turcomanica(with different concentrations)for 24,48 and72 hours.The effect of the extract on cell viability was evaluated by MTT method.Next,RNA extraction was performed and after cDNA synthesis,the expression level of Bax and Bcl2 genes was measured by Real-time PCR method.This research showed that with the increase in concentration and time,the viability of the cells decreased significantly compared to the control samples.Also, the results of real-time PCR showed that the expression of Bax and Bcl2 genes changed significantly compared to the control sample at 48 and72 hours.The results showed that the extract of Artemisia turcomanica has cytotoxic and apoptotic effects on the MCF-7 cell line, so that by conducting more studies, the extract of this plant can be used as an anti-cancer biological product in the treatment of cancer Manuscript profile -
Open Access Article
33 - اثرات محافظتی بتائین بر سکته قلبی ناشی از ایزوپرنالین از طریق apoE، Bcl-2 و عوامل آنتی اکسیداتیو در مدل موش صحرایی
سروش قدرتی زاده قلی نژاد قلی نژاد محمد حسن خادم انصاری یوسف رسمیبتائین، یک مولکول چند عملکردی که دارای ویژگی حفاظت قلبی است ولی مکانیسم آن به خوبی مشخص نیست. لذا در این مطالعه ما به بررسی اثر بتائین بر بافت قلب و بیومارکرهای سرمی پرداختیم. بتائین در دوز های 50، 150 و 250 میلی گرم بر کیلوگرم بدن به موش های صحرایی آلبینو داده شد. سکته Moreبتائین، یک مولکول چند عملکردی که دارای ویژگی حفاظت قلبی است ولی مکانیسم آن به خوبی مشخص نیست. لذا در این مطالعه ما به بررسی اثر بتائین بر بافت قلب و بیومارکرهای سرمی پرداختیم. بتائین در دوز های 50، 150 و 250 میلی گرم بر کیلوگرم بدن به موش های صحرایی آلبینو داده شد. سکته قلبی با تزریق 100 میلی گرم بر وزن رت القا شد. تست تانل و تست ایمنوهیستوشیمی به ترتیب برای بررسی آپوپتوز و سطح پروتئین نیتریک اکسید انجام شد. پراکسیداسیون لیپیدی و مقادیر آنزیم سوپر اکسید دیسموتاز با روش کالریمتری انجام شد. بیان ژن های آپوE و Bcl-2 در سلول های تک هسته ای خون محیطی با روش RT-PCR تعیین شد. نتایج نشان داد که تجویز بتائین در دوز 250 میلی گرم بر وزن بدن به مدت 60 روز سبب کاهش آپوپتوز پس از سکته قلبی می شود (0/038>P). ژن Bcl-2 در بافت قلب در دوز 150 میلی گرم بر وزن بدن القا شده و نتایج مشابهی برای آپوE مشاهده شد. در حالیکه تغیراتی در مقادیر نیتریک اکسید مشاهده نشد اما پراکسیداسیون لیپیدی و سطوح آنزیم سوپر اکسید دیسموتاز کاهش یافت. نتایج مطالعه ما حاکی از آن است که بتائین به کمک Bcl-2 و ویژگی ضد استرس اکسیداتیو، سبب حفاظت قلب می شود. Manuscript profile -
Open Access Article
34 - Luteolin Co-treatment abates Polystyrene Microplastics (PS-MPs) Induced Spermatotoxicity and Dysgonadogenesis in Rats Via up-regulation of Gonadotropin, Enhanced Spermatogenesis, Downregulation of Caspases, and Oxido-inflammation
Oyovwi Mega Obukohwo Ohwin Peggy EjiroPolystyrene microplastics (PS-MPs) pose a significant scientific concern due to their chemical makeup. Luteolin (LUT), a phytochemical-biological substance with anti-oxidative and anti-inflammatory properties, was tested against polystyrene microplastics (PS-MPs)-induce MorePolystyrene microplastics (PS-MPs) pose a significant scientific concern due to their chemical makeup. Luteolin (LUT), a phytochemical-biological substance with anti-oxidative and anti-inflammatory properties, was tested against polystyrene microplastics (PS-MPs)-induced reproductive toxicity in male Wistar rats. The rats were randomly assigned to 4 groups and treated as follows: Group I received 0.01 mL kg-1 of DMSO alone, Group II received 100 mg kg-1 of LUT dissolved in DMSO, Group III received 0.01 mg kg-1 of PS-MPs, and Group IV received 0.01 mg kg-1 of PS-MPs and 100 mg kg-1 of LUT. PS-MPs alone dramatically increased sperm morphological defects, xanthine oxidase, and lipid peroxidation while decreasing testicular enzymes, luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, antioxidants, and interleukin-10 (IL-10). The study demonstrates that PS-MPs can alter male rat testicular function and androgenicity. However, as a result of LUT co-treatment, sperm count, motility, and viability were increased whereas MDA and XO, IL-1β and TNF-α, and caspase-3 activities in the testis were reduced. Supplementing with LUT might act as a cytoprotective agent to lessen the spermatotoxicity and dysgonadogenesis brought on by PS-MPs. Manuscript profile -
Open Access Article
35 - The Effect of Nd-YAG Laser in Photodynamic Treatment on LN-CaP Cells
هما محسنی کوچصفهانی محمد نبیونی محمدحسین مجلس آرا الهه امینیPhotodynamic therapy (PDT) is a novel and promising method for cancer treatment.PDT is based on 3 factors including Photosensitizer (Ps), light with an appropriate wavelength and oxygen. Interaction between Ps and visible light can induce death ofcancer cells.The purpos MorePhotodynamic therapy (PDT) is a novel and promising method for cancer treatment.PDT is based on 3 factors including Photosensitizer (Ps), light with an appropriate wavelength and oxygen. Interaction between Ps and visible light can induce death ofcancer cells.The purpose of this study is evaluating combined effect of Aminolevulenic acid (5-ALA) as Ps and homogenous beam of solid state laser (Nd-YAG with speciall wavelength and power to induce death of prostate cancer cells and to determine the type of death.In this assay , using 0.3 mM 5-ALA (precursor of Protoporphyrin IX =PpIX as Ps) for 5h incubating period in dark and hemogenous beam of solid state laser (Nd-YAG with 532nm,50mW was evaluated viability of LN-CaP cells by MTT assay was evaluated formation of PpIX in cells. In addition type of cell death induced were determined using by hocsht staining .24h after treatment, significant reduction in number of cells in PDT groups compared to ALA group without laser and laser group without ALAwere seen. Administration of ALA were caused increased production of chief Ps(PpIX) in cells.Using hocsht staining apoptotic cell death in ALA-PDT groups were indicated.PDT treatment of LN-CaP cells with 0.3 mM 5-ALA for 5h incubatingperiod in dark and hemogenous beam of Nd-YAG laser with 532nm,50mW for 3 min caused increase PpIX formation and induced considerable apoptotic cell death. Manuscript profile -
Open Access Article
36 - Evaluation of Anti-Proliferative and Anti- Effects of Kombucha Tea Solvent Fraction on Colorectal Cancer Cell Line (HT-29)
Elham Ghodousi Dehnavi Mohammad Arjmand Reza Haji Hosseini Zahra Zamani Sima NasriFermented beverage, traditionally the result of the fermentation of sweet tea by Kombucha fungus, has beneficial effects in the treatment of many diseases, especially cancer. The present study is aimed at assessing the effect of different fractions of Kombucha tea on pr MoreFermented beverage, traditionally the result of the fermentation of sweet tea by Kombucha fungus, has beneficial effects in the treatment of many diseases, especially cancer. The present study is aimed at assessing the effect of different fractions of Kombucha tea on proliferation and apoptosis on colon cancer cell line HT-29. In the present experimental study, the survival rate and cell proliferation of Kumbucha tea solvent fractions including chloroform, ethyl acetate, butanol, hexane, and the final aqueous phase at concentrations of 0-900 μg/ ml were investigated by MTT and clonogenic assay. The rate of apoptosis induction was assessed by DNA fragmentation assay and flowcytometry. The aqueous and butanol fractions had no cytotoxic effects. After 24 hours, the IC50 for ethyl acetate, chloroform, and hexane was determined 213/49±1/63, 296/70±2/11 and 563/2±83/29 μg/ml, respectively. The results revealed that inhibition of cell growth was dose-dependent. The concentration of IC50 in these fractions caused the DNA to fragment. Apoptosis analysis by flowcytometry with these concentrations showed that the ethyl acetate component caused apoptosis while the death caused by IC50 concentrations of chloroform and hexane fractions was more than necrosis. The dose-dependent ethyl acetate fraction of Kambucha tea induced cell death through the apoptotic pathway and it was observed to be responsible for the anti-apoptotic and anti-proliferative properties. It seems to be a good candidate to prevent the proliferation of colorectal cancer cells. Manuscript profile -
Open Access Article
37 - The Effect of 12 Weeks of Green Tea Extract Consumption on Expression of Bax and bcl-2 Genes of Old ’Rats’ Heart Tissue in Response to Acute Exhaustive Exercise
Ahmad Kazemi Naser Behpoor Ahmad HematfarThe incidence of heart disease increases with age. Green tea is an antioxidant that can prevent cellular senescence. Mitochondria play a vital role in the intracellular pathway of apoptosis. Bax and bcl-2 proteins are the main regulators of mitochondrial apoptosis. Thus MoreThe incidence of heart disease increases with age. Green tea is an antioxidant that can prevent cellular senescence. Mitochondria play a vital role in the intracellular pathway of apoptosis. Bax and bcl-2 proteins are the main regulators of mitochondrial apoptosis. Thus, the present study is aimed at investigating the effect of 12 weeks of green tea extract consumption on expression Bax and bcl-2 genes of old rats’ heart tissue in response to acute exhaustive exercise. Thirty-two male rats were randomly assigned to two groups of supplement and non-supplement, each consisting of 16 rats. The supplement group received green tea extract for 12 weeks. At the end of the 12 weeks, each of these groups was further divided into two groups of rest and acute exhaustive exercise. The non-supplement with the rest group was called the control group. The non-supplement with exercise group was called exercise group, the supplement group with rest was called the supplement group and finally, the last group was entitled to supplement with exercise. After 12 weeks of taking green tea extract, one session of the acute exhaustive exercise was held on the treadmill. The real-time PCR method was used to measure the expression of Bax and bcl-2 genes. The results showed that 12 weeks of green tea extract consumption significantly reduced Bax gene expression and Bax to bcl-2 gene expression ratio and significantly increased cardiac tissue bcl-2 gene expression in response to acute exhaustive exercise. About acute exhaustive exercise session, it significantly increased Bax gene expression and Bax to bcl-2 gene expression ratio as well as significantly decreasing bcl-2 gene expression in heart tissue. The study concludes that 12 weeks of green tea extract can reduce Bax gene expression and Bax to bcl-2 gene expression ratio and significantly increase cardiac tissue bcl-2 gene expression at rest and after acute exhaustive exercise Manuscript profile -
Open Access Article
38 - The Apoptotic Effects of Testosterone on Lung Cancer (A549) Cells
Azita Tishehyar Rahim Ahmadi Minoo Mahmoudi Abdolreza MohamadniaStudies have shown that sex steroids affect the proliferation of cancer cells at cellular and molecular levels. The present study investigated the apoptotic effects of testosterone on lung cancer (A549) cells in cell culture medium. In this experimental-laboratory study MoreStudies have shown that sex steroids affect the proliferation of cancer cells at cellular and molecular levels. The present study investigated the apoptotic effects of testosterone on lung cancer (A549) cells in cell culture medium. In this experimental-laboratory study, the cytotoxic effects of testosterone on Hek293 and A549 cells were assayed using MTT method. Real time PCR was used to evaluate the expression levels of Bax and Bcl2 genes in A549 cells exposed to IC50 dose of testosterone. The data were statistically analyzed between groups using one-way ANOVA. Exposure of Hek293 and A549 cells to higher dose of testosterone (1000 microg/ml) of testosterone resulted in significant decrease in cell viability (P<0.001). IC50 dose of testosterone significantly decreased the anti-apoptotic Bcl-2 gene expression level (P<0.001) in A549 cells, however, did not significantly change the expression level of apoptotic Bax gene. The cytotoxic concentration of testosterone induces apoptosis in lung cancer cells by its inhibitory effect on anti-apoptotic Bcl-2 gene expression level. Accordingly, appropriate dose of testosterone has anti-cancer effects against lung cancer cells. Manuscript profile -
Open Access Article
39 - The Effect of Avicennia marina Flavonoids on Bax, Bcl-2 and Stress Oxidation Indicators of Epididymis Sperm in Type 1 Diabetic Rats
Raheleh RahbarianDiabetes affects the reproductive system and causes fertility disorders. Regarding the anti-oxidant and hypoglycemic properties of A. marina, the aim of this study was to determine the effect of total flavonoid contents on Bax, Bcl-2 level, and stress-oxidative stress i MoreDiabetes affects the reproductive system and causes fertility disorders. Regarding the anti-oxidant and hypoglycemic properties of A. marina, the aim of this study was to determine the effect of total flavonoid contents on Bax, Bcl-2 level, and stress-oxidative stress indices of epididymis sperm in type 1 diabetic rats. In this experimental study, 32 male Wistar rats were divided into control, control diabetic, and two diabetic treated groups. The last two groups received 50 and 100 mg / kg Flavonoids in A. marina leaf for 30 days. At the end of the treatment period, sperms were extracted from the epididymis. Then Bax, Bcl-2, malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, and HOdG levels in sperm samples also FBS were measured by ELISA method. According to the results, the levels of Bcl-2, superoxide dismutase, glutathione peroxidase and catalase in sperm samples of diabetic rats treated with 50 and 100 mg / kg flavonoid concentrations of A. marina in Comparison with the control group, the diabetic was significantly increased, and the Bax, malondialdehyde and HOdG levels decreased significantly, Depending on the injectable dose (p <0.05). Flavonoid administration of A. marina leaves decreased apoptosis and stress-oxidative stress in spermatozoa of type I diabetic rats. Manuscript profile -
Open Access Article
40 - The Effect of a Short Period of Detraining Following the Six Weeks of High Intensity Interval Training on HIF-1α Levels and Bronchus and Bronchioles Apoptosis in Lung Tissue in Male Wistar Rats
Saber Niazi Shadmehr Mirdar Reza Bazzar Gholamreza HamidianThis study aimed evaluate the effect of short-term detraining following 6 weeks of high-intensity interval training on HIF-1α, bronchus, and bronchioles apoptosis levels on lung tissue in male Wistar rats. For this purpose, 18 healthy Wistar male rats (4 weeks wit MoreThis study aimed evaluate the effect of short-term detraining following 6 weeks of high-intensity interval training on HIF-1α, bronchus, and bronchioles apoptosis levels on lung tissue in male Wistar rats. For this purpose, 18 healthy Wistar male rats (4 weeks with a mean weight of 72±9.9 g) were divided into experimental (n=12) and control (n=6) groups. After 6 weeks of high-intensity interval training, 6 rats the experimental group was transferred to the detraining group (n=6) and kept in detrain condition for three weeks. To measure of HIF-1α and bronchus and bronchioles apoptosis, a lung tissue sample was taken and measured at the end of the ninth week. One-way analysis of variance at the significance level (P≤0.05) was used to analyze the data. The results showed that despite the significant increase in HIF-1α and bronchial apoptosis and pulmonary bronchioles (P≤0.05) during high-intensity training, there was a significant decrease (P ≤ 0.05) in HIF-1α, bronchial apoptosis and bronchioles of lung tissue were observed. It seems to be done by the increase of the amount of HIF-1α and apoptosis in multiple cell lung bronchi and bronchioles, but the detraining period will decrease.. Manuscript profile -
Open Access Article
41 - The effect of high intensity interval training and Adiantum capillus-veneris extract supplementation on HIF-1α levels and bronchial and bronchiole apoptosis in lung tissue of male Wistar rats
Saber Niazi shadmehr MirdarHigh intensity interval training is associated with impaired immune function and cellular damage in lung tissue. Adiantum capillus-veneris has been considered as an herbal supplementation in traditional medicine as an antioxidant. Therefore, the present study investigat MoreHigh intensity interval training is associated with impaired immune function and cellular damage in lung tissue. Adiantum capillus-veneris has been considered as an herbal supplementation in traditional medicine as an antioxidant. Therefore, the present study investigated the effect of high-intensity interval training on HIF-1α levels and the rate of bronchial and bronchiole apoptosis in lung tissue. For this purpose, 24 healthy Wistar male rats (4 weeks with a mean weight of 72 ± 9 g) were divided into experimental (n = 18) and control (n = 6) groups. After 6 weeks of high-intensity interval training, 6 rats in the experimental group were transferred to the supplement group (n = 6), continued training (n = 6) and exercise supplement (n = 6), and were monitored for another three weeks. At the end of the ninth week, lung tissue samples were taken and assayed to measure HIF-1α levels and bronchial and bronchiole apoptosis. One-way analysis of variance at the significant level (p ≥ 0.05) was used to analyze the data. The results showed that despite a significant increase in HIF-1α and bronchial apoptosis and pulmonary bronchioles (p ≥ 0.05) following increased exercise activity of Adiantum capillus-veneris, herbal supplementation in both supplemental group alone and exercise supplementation was significantly decreased. (p ≥ 0.05) associated with HIF-1α, bronchial apoptosis and bronchial lung tissue. Therefore, Adiantum Capillus-Veneris can lead to a decrease in HIF-1α levels and associated bronchial apoptosis and pulmonary bronchioles. Manuscript profile -
Open Access Article
42 - Apoptosis induction of Harmine on Human Colon Cancer Cell Line HT29 and Alteration in Apoptotic Genes Expression P53, Bax and Bcl-2
Reyhaneh Chitbandi Javad Baharara Maryam TehranipourAlkaloids have anti-proliferative and apoptotic stimulatory effects on cancer cells. Harmine is also one of the important alkaloids that have been reported to have anticancer effects. The aim of this study was to investigate the effect of Harmine on apoptosis induction MoreAlkaloids have anti-proliferative and apoptotic stimulatory effects on cancer cells. Harmine is also one of the important alkaloids that have been reported to have anticancer effects. The aim of this study was to investigate the effect of Harmine on apoptosis induction in human HT29 colon cancer cell line and expression alteration of apoptotic genes P53, Bax and Bcl-2. For this purpose, HT29 colon cancer cells were treated with different concentrations of harmine (0, 35, 45, 55, 65 μg/ml) for 24 hours. Then the cell viability was assessed using the MTT assay. Nuclear changes and chromatin condensation were investigated by DAPI staining. Also, apoptosis induction was determined by Annexin V-FITC testing and changes in P53, Bax and Bcl-2 genes expression was assessed by using Real Time PCR. The results showed that the concentration of Harmin reduced the viability of HT29 colon cancer cells. Also, the treatment of HT29 colon cancer cells with Harmin caused morphological changes in the cell nucleus, including chromatin condensation of the nucleus, the formation of apoptotic bodies, and the wrinkling of the cell membrane. All of these morphological features indicate apoptosis. The results of the Annexin test also confirmed this finding; with about 57% of the cells undergo apoptosis. On the other hand, the treatment of HT29 cancer cells with Harmin reduced the expression of Bcl-2 and at the same time increased the expression of P53 and Bax and, in general, reduced the ratio of Bcl-2 to Bax. As a result, Harmin can be used to treat colon cancer cells because of its cytotoxicity, which is effective in inhibiting cell prolifration and inducing apoptosis. Manuscript profile -
Open Access Article
43 - The Effect of Cobalt-oxide Nanoparticles Conjugated with Glutamic Acid and Thiosemicarbazone on Apoptosis of Hepatocellular Carcinoma Cancer Cells
Asal Shahrokhshahi Ali Salehzadeh Hamidreza Vaziri Zeinab Moradi ShoeiliCancer is the second most common cause of death after cardiovascular disease. The present study was aimed at assessing the cobalt oxide nanoparticles conjugated with thiosemicarbazone (CO3O4-TSC) against hepatic cancer cells HepG2 in vitro. The CO3O4-TSC nanoparticles w MoreCancer is the second most common cause of death after cardiovascular disease. The present study was aimed at assessing the cobalt oxide nanoparticles conjugated with thiosemicarbazone (CO3O4-TSC) against hepatic cancer cells HepG2 in vitro. The CO3O4-TSC nanoparticles were synthesized by chemical and condensation methods. The effect of different concentrations of nanoparticles on the growth of Hek392 normal and HepG2 cancerous cells was assessed by MTT assay. The effect of CO3O4-TSC nanoparticles on apoptosis was assessed by measuring apoptotic Bax and anti-apoptotic Bcl2 genes using specific primers with the Real-Time PCR method. Mean expression of Bax gene in nanoparticle-treated cancer cells (2.19 ± 0.091) was significantly higher than normal cells (1.0 ± 0.027) and untreated cancer cells (0.84 ± 0.047) (p <0.001). The mean expression of Bcl2 gene was significantly lower in nanoparticle-treated cancer cells (0.65 ± 0.033) than in normal (1.0 ± 0.017) and untreated cancer cells (1.38 ± 0.033) (p <0.001). The expression ratio of Bax/Bcl2 in nanoparticle-treated cancer cells (3.36) was significantly higher than normal (1.0) and untreated cancer cells (0.61) (p <0.001). CO3O4-TSC nanoparticle has a high cytotoxic effect on cancer cells, possibly mediated by the induction of apoptotic Bax expression and decreased expression of anti-apoptotic Bcl2 gene. Manuscript profile -
Open Access Article
44 - Effect of ZnFe2O4@Ag Nanocomposite Biosynthesized by Chlorella vulgaris on the Expression of P53, Caspase 9, and CAD Genes in Breast Cancer Cell Line
Ayda Mohammad Amoie Vajiheh Zarrinpour Seyed Ataollah Sadat Shandiz Ali SalehzadehRegarding the increasing trend of the morbidity and mortality of breast cancer, the use of nanotechnology products in breast cancer treatment has gained interest. The current work aims to synthesize ZnFe2O4@Ag nanocomposite using the Chlorella vulgaris extract, assessme MoreRegarding the increasing trend of the morbidity and mortality of breast cancer, the use of nanotechnology products in breast cancer treatment has gained interest. The current work aims to synthesize ZnFe2O4@Ag nanocomposite using the Chlorella vulgaris extract, assessment of its anticancer potential on breast cancer cells, and evaluate the effect of the nanocomposite on the expression of apoptotic genes. Anticancer potential and lethal dose 50% (IC50) of ZnFe2O4@Ag were determined using the MTT assay and real-time PCR was employed to evaluate the expression of p53, caspase 9, and CAD genes. According to the results, at concentrations greater than 15.62 µg/mL, ZnFe2O4@Ag considerably decreased the viability of breast cancer cells and IC50 was determined 28µg/mL. Moreover, the relative expression of the p53, caspase 9, and CAD genes was significantly increased by 2.22, 1.98, and 5.96 folds, respectively after exposure to the nanocomposite. Owing to the presence of silver and zinc, it seems that ZnFe2O4@Ag generates reactive oxygen species that could damage cell components and subsequently induce cell apoptosis. Manuscript profile -
Open Access Article
45 - Effect of Lycium depressum Root on Bax , Bcl-2 Gene Expressions and Evaluation of Caspase 3 and 6 Activity on Uterine Cervical Cancer cells line (Hela)
Yasamin Khani Tahereh Naji Soheila Zamanlui Benisi Saeid Mohamadi MotamedCervical cancer is the third cause of death caused by cancer in women. After breast cancer, cervical cancer is the most common cancer in women. Today, due to the numerous side effects of chemotherapy and radiation therapy that are caused to the patient as a result of th MoreCervical cancer is the third cause of death caused by cancer in women. After breast cancer, cervical cancer is the most common cancer in women. Today, due to the numerous side effects of chemotherapy and radiation therapy that are caused to the patient as a result of their use, as well as the resistance of cancer cells to common treatments, researchers have turned to new drugs with greater effectiveness and less toxicity. The aim of the present study was to evaluate the effect of Lycium depressum root on the expression of Bcl2 and Bax genes and to evaluate the activity of caspases 3 and 6 in cervical cancer cells (Hela). In this study, firstly, the extract of Lycium depressum root was obtained by soaking method, then cervical cancer cells were prepared and cultured, in order to calculate the optimal effective dose of the extract, the MTT technique was used. The concentrations of 25 and 50 mg/ml were chosen as optimal doses. Then the expression of Bcl2 and Bax genes was evaluated by Real-time PCR technique. Next, in order to evaluate the activity of caspases 3 and 6 in effective doses of the extract, cervical cancer cells (Hela) were affected and protein expression was measured using the immunocytochemistry (ICC) technique. The results of this research showed that the roots of the Turkmen oleander plant can cause changes in the expression of important Bcl2 and Bax genes in the path of apoptosis induction. In addition, it increases the protein expression of caspase 3 and 6 in cancer cells. Therefore, it can be hoped that in additional research on the extract of Lycium depressum root, it will be considered a candidate for the treatment of complementary medicine. Manuscript profile
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