Investigation of the anticancer properties of iron oxide nanoparticles conjugated with coumarin and hyaluronic acid in breast cancer cell line (MCF-7)
Subject Areas : cellular and molecular bilologyJavad Solati 1 , Ali Salehzadeh 2
1 - Department of Biology, Ra. C., Islamic Azad University, Rasht, Iran
2 - Department of Biology, Ra. C., Islamic Azad University, Rasht, Iran
Keywords: Apoptosis, Breast cancer, Coumarin, Hyaluronic acid,
Abstract :
Introduction: Treatment of breast cancer is difficult due to the diverse, drug-resistant and metastatic types of the disease. New pharmaceutics have been focused on the specific targeting of cancer cells to improve the efficacy of chemotherapy. This study aimed to investigate the anticancer effects of iron oxide nanoparticles conjugated with coumarin and hyaluronic acid (Fe3O4@Glu-CO-HA) on breast cancer cell line (MCF-7).
Materials and Methods: Fe3O4 nanoparticles were chemically synthesized and conjugated with hyaluronic acid and coumarin. The physicochemical properties of the nanoparticles were evaluated by FT-IR, XRD, zeta potential and SEM and TEM imaging. The inhibitory effects of nanoparticles on cancer cells were investigated by MTT assay for 24 and 48 hours and flow cytometry analysis was used to determine the frequency of apoptotic/necrotic cells.
Results: According to the results, iron oxide nanoparticles were synthesized correctly and conjugated with hyaluronic acid and coumarin. The particles had a spherical shape with a size of 10-60 nm and a surface charge of -11mV. Fe3O4@Glu-CO-HA nanoparticles exhibited concentration- and time-dependent inhibitory effects on cancer cells, and the 24- and 48-hour 50% inhibitory concentration were 111 and 63μg/mL, respectively. Treatment of MCF-7 cells with Fe3O4@Glu-CO-HA nanoparticles caused a sharp increase in the percentage of apoptotic and necrotic cells.
Conclusion: This study showed significant anticancer effects of Fe3O4@Glu-CO-HA nanoparticles on the breast cancer cell line. The observed anticancer effects can be attributed to the targeting of cancer cells due to the presence of hyaluronic acid on the nanoparticle surface as well as the anticancer effects of coumarin.
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