The effect of HIIT and thyme honey on gene expression of cardiac tissue apoptotic indices and insulin resistance index in type 2 diabetic rats

Subject Areas :

bahareh  behaeen ¹ (Department of Islamic Azad University, Dariun Branch, Dariun, Iran)
Hossein  َAbedntanzi ² (Department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran.)
mandana  gholami ³ (Department of physical education and sport science, Science and research branch, Islamic Azad University, Tehran, Iran.)
farshad  ghazalian ⁴ (Department of Physiology, Faculty of Physical Education and Sport Sciences, Azad University, Research Sciences Branch, Tehran, Iran)

Received: 2021-08-12 Accepted : 2021-08-24 Published : 2022-02-20

Keywords: Apoptosis, HIIT, Type 2 diabetes, Insulin Resistance Index, Thyme Honey,

Abstract :

Inroduction & Objective: Type 2 diabetes is the most common endocrine disease that can cause damage and cell death or apoptosis. The aim of this study was to investigate the expression of apoptotic and anti-apoptotic gene of heart tissue and insulin resistance index after intense intermittent exercise and consumption of thyme honey in diabetic type 2 rats. Materials and Methods: The statistical population consisted of rats. After 20 weeks of high fat diet and injection of STZ became diabetic. Rats in 4 groups: control(n=6),HIIT(n= 8),thyme honey(n= 6),HIIT-thyme honey (n=8) trained HIIT for eight weeks, five sessions per week with intense 2-minute intense alternation with 2 to 8 alternations and with 80 to 90% vo2max and one-minute rest alternation with 50 to 56% vo2max.Thyme honey was given by gavage at a rate of 3g/kg 5days a week. Glucose, insulin and expression of Bax and Bcl2 genes and their ratio was calculated. Statistical analysis was performed using two-factor analysis of variance test and determining the effect size and Bonfroni post hoc.Results: HIIT and thyme honey decreased Bax gene expression and increased Bcl2 expression in heart cells (P<0.05). HIIT and thyme honey in diabetic rats led to improved glucose and insulin levels and decreased insulin resistance index. It also decreased the expression of Bax gene and increased the expression of Bcl2 (P<0.05). Conclusion: HIIT with thyme honey led to improved glycemic profile and changes in glucose and insulin levels, as well as positive and appropriate changes in the expression of cardiac and anti-apoptotic genes. 

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