تاثیر تمرین تناوبی شدید و عسل آویشن بر بیان ژن شاخص‌های آپوپتوزی بافت قلب و شاخص مقاومت به انسولین در رت‌های دیابتی نوع دو

محورهای موضوعی : مجله پلاسما و نشانگرهای زیستی

بهاره به آئین ¹ (هیات علمی دانشگاه آزاد اسلامی واحد داریون)
حسین عابد نطنزی ² (گروه تخصصی تربیت بدنی و علوم ورزشی،دانشگاه آزاد اسلامی،واحد علوم و تحقیقات، تهران، ایران.)
ماندانا غلامی ³ (هیات علمی دانشگاه آزاد اسلامی واحد علوم و تحقیقات)
فرشاد غزالیان ⁴ (گروه فیزیولوژی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه آزادواحد علوم تحقیقات، تهران، ایران)

تاریخ دریافت : 1400/05/21 تاریخ پذیرش : 1400/06/02 تاریخ انتشار : 1400/12/01

کلید واژه: شاخص مقاومت به انسولین, آپوپتوز, ‌عسل آویشن, دیابت نوع دو, تمرین تناوبی شدید,

چکیده مقاله :

زمینه و هدف: دیابت نوع 2 شایع ترین بیماری غدد درون ریز است که می‌تواند باعث آسیب و مرگ سلولی یا آپوپتوز شود. هدف پژوهش حاضر مطالعه تغییرات بیان ژن آپوپتوزی و آنتی‌آپوپتوزی بافت قلب و شاخص مقاومت ‌به ‌انسولین پس از تمرین تناوبی شدید و مصرف عسل آویشن در رت‌های دیابتی نوع دو بود.روش کار: جامعه آماری را موش‌های صحرایی نر تشکیل می دادند که پس از 20 هفته تغذیه با رژیم پرچرب و با تزریق STZ دیابتی شدند. موش‌ها در چهار گروه کنترل دیابتی شش، تمرین تناوبی هشت، عسل آویشن شش، تمرین تناوبی‌ و عسل آویشن هشت سرگروه‌بندی شدند و هشت هفته تحت تمرین تناوبی،پنج جلسه در هفته با تناوب شدید دو دقیقه‌ای با دو تا هشت تناوب و 80-90% vo2max و استراحت یک دقیقه‌ای با 50 تا 56% vo2max قرار گرفتند. عسل آویشن به صورت گاواژ، سه گرم برکیلوگرم پنج روز در هفته داده شد. گلوکز، انسولین و بیان ژن Bax و Bcl2 و BAX/Bcl2 اندازه گیری شد. تحلیل آماری با استفاده آزمون تحلیل واریانس دو عاملی و تعیین اندازه اثر و تعقیبی بن فرونی انجام شد. یافته ها: HIIT به کاهش معنی‌دار گلوکز و شاخص مقاومت به انسولین منجر شد. تمرین تناوبی و مصرف عسل -آویشن هم چنین به کاهش بیان Bax و افزایش بیان Bcl2 منجر گردید(P<0.05). تمرینات تناوبی همراه با مصرف عسل ‌آویشن در رت‌های دیابتی منجر به بهبود سطوح گلوکز و انسولین و کاهش شاخص مقاومت به انسولین و نیز باعث کاهش بیان Bax و افزایش Bcl2 در سلول های قلبی نسبت به گروه کنترل شد(P<0.05).نتیجه گیری: تمرین تناوبی و مصرف عسل آویشن به بهبود پروفایل گلایسمیک و هم چنین تغییرات مثبتی در بیان ژن‌های قلبی و ضد آپوپتوزی منجر می‌شود.

چکیده انگلیسی:

Inroduction & Objective: Type 2 diabetes is the most common endocrine disease that can cause damage and cell death or apoptosis. The aim of this study was to investigate the expression of apoptotic and anti-apoptotic gene of heart tissue and insulin resistance index after intense intermittent exercise and consumption of thyme honey in diabetic type 2 rats. Materials and Methods: The statistical population consisted of rats. After 20 weeks of high fat diet and injection of STZ became diabetic. Rats in 4 groups: control(n=6),HIIT(n= 8),thyme honey(n= 6),HIIT-thyme honey (n=8) trained HIIT for eight weeks, five sessions per week with intense 2-minute intense alternation with 2 to 8 alternations and with 80 to 90% vo2max and one-minute rest alternation with 50 to 56% vo2max.Thyme honey was given by gavage at a rate of 3g/kg 5days a week. Glucose, insulin and expression of Bax and Bcl2 genes and their ratio was calculated. Statistical analysis was performed using two-factor analysis of variance test and determining the effect size and Bonfroni post hoc.Results: HIIT and thyme honey decreased Bax gene expression and increased Bcl2 expression in heart cells (P<0.05). HIIT and thyme honey in diabetic rats led to improved glucose and insulin levels and decreased insulin resistance index. It also decreased the expression of Bax gene and increased the expression of Bcl2 (P<0.05). Conclusion: HIIT with thyme honey led to improved glycemic profile and changes in glucose and insulin levels, as well as positive and appropriate changes in the expression of cardiac and anti-apoptotic genes. 

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