The Effect of Nd-YAG Laser in Photodynamic Treatment on LN-CaP Cells
Subject Areas : Journal of Animal Biology
هما محسنی کوچصفهانی
1
(
استادیار گروه زیست شناسی دانشکده علوم دانشگاه تربیت معلم تهران
)
محمد نبیونی
2
(
استادیار گروه زیست شناسی دانشکده علوم دانشگاه تربیت معلم تهران
)
محمدحسین مجلس آرا
3
(
دانشیار گروه فیزیک دانشکده علوم دانشگاه تربیت معلم تهران
)
الهه امینی
4
(
دانشجوی کارشناسی ارشد سلولی تکوینی جانوری دانشگاه تربیت معلم تهران
)
Keywords: Apoptosis, Photodynamic therapy (PDT), Ala, Nd-YAG laser, PpIX,
Abstract :
Photodynamic therapy (PDT) is a novel and promising method for cancer treatment.PDT is based on 3 factors including Photosensitizer (Ps), light with an appropriate wavelength and oxygen. Interaction between Ps and visible light can induce death ofcancer cells.The purpose of this study is evaluating combined effect of Aminolevulenic acid (5-ALA) as Ps and homogenous beam of solid state laser (Nd-YAG with speciall wavelength and power to induce death of prostate cancer cells and to determine the type of death.In this assay , using 0.3 mM 5-ALA (precursor of Protoporphyrin IX =PpIX as Ps) for 5h incubating period in dark and hemogenous beam of solid state laser (Nd-YAG with 532nm,50mW was evaluated viability of LN-CaP cells by MTT assay was evaluated formation of PpIX in cells. In addition type of cell death induced were determined using by hocsht staining .24h after treatment, significant reduction in number of cells in PDT groups compared to ALA group without laser and laser group without ALAwere seen. Administration of ALA were caused increased production of chief Ps(PpIX) in cells.Using hocsht staining apoptotic cell death in ALA-PDT groups were indicated.PDT treatment of LN-CaP cells with 0.3 mM 5-ALA for 5h incubatingperiod in dark and hemogenous beam of Nd-YAG laser with 532nm,50mW for 3 min caused increase PpIX formation and induced considerable apoptotic cell death.
