The effect of high intensity interval training and Adiantum capillus-veneris extract supplementation on HIF-1α levels and bronchial and bronchiole apoptosis in lung tissue of male Wistar rats

Subject Areas : Journal of Animal Biology

Saber Niazi ¹ , shadmehr Mirdar ²

1 - Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
2 - Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Mazandaran, Iran

Received: 2021-08-09 Accepted : 2021-11-12 Published : 2022-11-22

Keywords: Apoptosis, High-intensity interval training, Lung tissue, Adiantum capillus-veneris,

Abstract :

High intensity interval training is associated with impaired immune function and cellular damage in lung tissue. Adiantum capillus-veneris has been considered as an herbal supplementation in traditional medicine as an antioxidant. Therefore, the present study investigated the effect of high-intensity interval training on HIF-1α levels and the rate of bronchial and bronchiole apoptosis in lung tissue. For this purpose, 24 healthy Wistar male rats (4 weeks with a mean weight of 72 ± 9 g) were divided into experimental (n = 18) and control (n = 6) groups. After 6 weeks of high-intensity interval training, 6 rats in the experimental group were transferred to the supplement group (n = 6), continued training (n = 6) and exercise supplement (n = 6), and were monitored for another three weeks. At the end of the ninth week, lung tissue samples were taken and assayed to measure HIF-1α levels and bronchial and bronchiole apoptosis. One-way analysis of variance at the significant level (p ≥ 0.05) was used to analyze the data. The results showed that despite a significant increase in HIF-1α and bronchial apoptosis and pulmonary bronchioles (p ≥ 0.05) following increased exercise activity of Adiantum capillus-veneris, herbal supplementation in both supplemental group alone and exercise supplementation was significantly decreased. (p ≥ 0.05) associated with HIF-1α, bronchial apoptosis and bronchial lung tissue. Therefore, Adiantum Capillus-Veneris can lead to a decrease in HIF-1α levels and associated bronchial apoptosis and pulmonary bronchioles.

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