Study on the apoptosis and Histopathology of the Heart following Renal Ischemia Time-Dependent Reperfusion in Male Rats

Subject Areas : Veterinary Clinical Pathology

Mahbod Bazhban ¹ , , Yousef Doustar ² , M.A Nourazar ³

1 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran
2 - Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
3 - Assostant Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.

Received: 2024-04-30 Accepted : 2024-08-26 Published : 2025-03-03

Keywords: ischemia-reperfusion, kidney, heart, rat, Apoptosis.,

Abstract :

Kidney ischemia-reperfusion injury (I/R) is one of the most important causes of acute renal failure and subsequent damage to other organs such as the heart. was to investigate the occurrence of damage and apoptosis in the heart following renal ischemia-reperfusion time-dependent injury in rats. For this purpose, 32 male Wistar rats were randomly divided into 4 equal groups. In the first group (Sham), only the surgical site was opened and closed. In the second group (30-I/R) after the induction of renal ischemia, reperfusion was performed 30 minutes later, in the third group (45-I/R) 45 minutes later, and in the fourth group (60-I/R) 60 minutes later. After 24 hours, all animals were euthanized and their kidney and heart tissues were sampled for microscopic study. Statistical analysis of the data was done by one-way analysis of variance (ANOVA) and Tukey's post-test, and values of p<0.05 were considered significant.Histopathological findings showed renal damage following I/R in the form of degenerative changes and necrosis of proximal and distal tubules, glomerular and interstitial tissue damage in a time-dependent manner. Also, renal I/R resulted in heart tissue damage including degenerative changes and necrosis along with apoptosis of cardiomyocytes in all groups, but the severity of tissue damage and apoptosis of cardiomyocytes in the 60-I/R group was significantly higher than other groups (p<0.05). The results of the study showed that by reducing the duration of renal ischemia, the damages to the kidney tissue and subsequently heart tissue as well as apoptosis of cardiomyocytes will be less.

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