The effect of endurance training and consumption of nettle extract on the gene expression of BCL-2 and TNF-α in mice with melanoma

Subject Areas :

lila  mosavi ¹ (PhD Student in Sport Physiology, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.)
barari  barari ² (department of sport physiology,ayatollah amoli branch ,islamic azad university, amol)
asieh  Abbasi Daloii ³ (Associate Professor, Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.)
hossien  Abed Natanzi ⁴ (Assistant professor, Department of Sport Physiology, science and research Branch, Islamic Azad University, tehran, Iran)

Received: 2021-09-06 Accepted : 2021-11-23 Published : 2022-02-20

Keywords: Melanoma Cancer, Bcl-2, nettle extract, TNF-α, Apoptosis,

Abstract :

Inroduction & Objective: : Melanoma is the most serious type of skin cancer, which is made up of melanin pigment cells.The aim of this study was to evaluate the effect of endurance training and nettle consumption on BCL-2 and TNF-α gene expression in mice with melanoma.Material and Methods: The subjects included male Wistar rats that after two weeks of adjustment were randomly divided into 4 groups including: control, exercise, extract and exercise + extract. Exercise program included 30 minute of running on a treadmill was steep and at a speed of 16 meters per minute for the first week, and every week one meter per minute was added until the eighth week it reached 22 meters per minute. One week after melanoma induction, the experimental group consumed 30 mg / kg / day of nettle ethanol extract orally for 8 weeks. RT PCR was used to measure gene expression.Results: Data analysis showed that consumption of nettle extract and endurance training significantly reduced the expression level of BCL-2 and TNF-α genes in mice with melanoma in different groups (p = 0.000, p = 0.025, respectively). The results also showed that the difference in the level of changes in BCL-2 and TNF-α gene expression between the exercise and extract group with the control group (p = 0.002, p = 0.014, respectively) was significantly reduced.Conclusion: Regular physical activity combined with consumption of nettle extract can probably reduce anti-apoptotic factors and cause apoptosis in cancer cells by reducing inflammatory factors.

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