A survey on the effects of citrate on canine mammary tumor cells (CF41-Mg)

Subject Areas : Journal of Comparative Pathobiology

Vahidi, R., Farsinejad, A., Safi, S. . ¹

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Received: 2017-02-12 Accepted : 2017-02-12 Published : 2017-01-20

Keywords: Citrate, Apoptosis, Canine mammary tumor,

Abstract :

Mammary tumors are among the most prevalent tumors in female dogs which according to the pathological classification have several types. These tumors have many resemblances to human tumors and can be used as a suitable model for study of human mammary tumors. Although the antitumor effects of citrate against a variety of cancerous cells has been proven, the effect of this substance against canine mammary tumor cells has not yet been analyzed. The aim of present study was to evaluate the apoptotic and cytotoxic effects of citrate on canine mammary tumor cells(CF41-Mg) by Annexin-PI and MTT tests (a yellow tetrazole is reduced to purple formazan in living cells), respectively. MTT data showed dose dependant inhibition of citrate on proliferation of CF41-Mg cells and 50%inhibitory concentration (IC50) of this substance at a dose of26mM. In addition, percentage of apoptotic cells significantly increased after 48 hours’ treatment with IC50 of citrate (P<0/001). According to the obtained results, it seems that citrate can be used as a suitable adjuvant therapy alongside other cancer treatment substances.