Comparison of apoptosis in canine transmissible veneral tumor (TVT) pre and post chemotraoy with vincristine sulphate
Subject Areas : Veterinary Clinical Pathologyیوسف Doustar 1 , داریوش Mohajeri 2 , رامین Kafash Elahi 3
1 - Assistant Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University- Tabriz Branch, Tabriz, Iran
2 - Associate Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University- Tabriz Branch, Tabriz, Iran
3 - Assistant Professor, Department of Small Animal Internal Medicine, Faculty of Veterinary Medicine, Islamic Azad University- Tabriz Branch, Tabriz, Iran
Keywords: Canine transmissible venereal tumor, Vincristine sulphate and apoptosis,
Abstract :
The canine transmissible veneral tumor (CTVT) is a prevalent tumor in canidae. It is transmitted by coitus, forming multiple neoplastic masses on the external genitalia of both sexes within the family canidae. CTVT have an aberrant karyotype and the origin of the neoplastic cells is undetermined but immunophenotyping suggests that the tumor has a histocytic origin. In this study 10 dogs with canine transmissible veneral tumor were selected and received vincristine sulphate (0.025 mg/kg/b.w) chemotrapy to induce apoptosis in neoplastic cells. Biopsy specimens were collected from tumors during the growth phase, before and again after chemotherapy from the same dogs. The specimens were fixed in 10% formalin and then prepared routinely for H&E and TUNEL assays. Histopathological study of tissue section of CTVT before chemotherapy revealed sheets of uniform neoplastic cells, round to oval in shape with defined cytoplasmic border. There were a few TUNEL positive cells and mitotic figures. In tumor specimens after chemotherapy increased TUNEL positive cells and depilation of neoplastic cells in stroma of tumor were observed. Mean deference of histopathological changes and TUNEL positive cells before and after chemotherapy were significant (p<0.003). This study indicates that vincristine sulphate is capable of induction of apoptosis in neoplastic cells of CTVT.
- دوستار، ی. 1383. مطالعه آزمایشی آپوپتوز القاء شده توسط ویروس عامل بیماری بورس عفونی جوجهها با استفاده از متد تشخیصی TUNEL و میکروسکوپ الکترونی. پایاننامه جهت دریافت دکترای تخصصی دانشگاه تهران، دانشکده دامپزشکی، شماره 189.
- Banguley, B.C., Holdaway, K.M. and Thomson, L.L. 1991. Inhibition of growth of colon 38 adernocarcinoma by vinblastine and colchicines: evidence for a vascular mechanism. Eur J Cancer. 27:482-487.
- Batamuzi, E.K., Bittegeko, S.B.P. 1991. Anal and perianal transmissible venereal tumor in a bitch. Veterinary Record. 129:556.
- Dong, L.i., Seong, H.J. and Jonghan, K. 2001. Enhanced drug-induced apoptosis associated with P-Glycoprotein overexpression is specific to antimicrotubule agents. Pharmaceutical Res. 20:45-50.
- Hill, S.A., Lonergan, S.J. and Denekam, P.J. 1993. Vinca alkaloids: anti-vascular effects in a murine tumour. Eur. J. Cancer. 29:1320-1324.
- Mukaratirwa, S. and Gruys, E. 2004. Canine transmissible veneral tumour:cytogenetic origins immunophenotypes and immunobiology.Published in veterinary quarterly. 25:101-111.
- Marchal, T., Chabanne, L. and Kaplanski, C. 1997. Immunophenotype of the canine transmissible venereal tumour. Veterinary Immunology and Immunopathology. 57:1-11.
- Michelle, T., Clodagh, E. and Finnegan, K.M. 2004. A modulator of chemotherapy-induced apoptosis. Cancer Research. 64:8357-8364.
- Park Soo-jang, Wu ching-Huang and Najafi F. 2006. P-Glycoprotein enhances TRAIL-triggered apoptosis in multidrug restant cancer cells by interacting with the death receptor DR5. Biochemical Pharmacology. 72:293-307.
- Takano, Y., Okudaira, M. and Harman, D. 1993. Apoptosis induced by microtubule disrupting drugs in cultured human lymphoma cells; inhibitory effects of phorbol ester and zing sulphate. Cancer Research. 189:197-203.
- Yee-Shin Lin and Chiou-Feng Lin. 2007. Ceramide in apoptotic signaling and anticancer therapy. NCKU Medicine. 18:7-10.
