List of articles (by subject) Aquatic Products

    • Open Access Article

      1 - Effect of sodium alginat and cinnamon essential oil on shelf life of silver carp (Hypophthalmichthys molitrix) fillets in refrigerated storage
      الهام آل طیب لاله رومیانی منصوره قائنی
      In this study was conducted to investigate the effect of sodium alginate and cinnamon essential oils on shelf life of fillets of silver carp (Hypophthalmichthys molitrix) fillets in refrigerated storage. Fish fillets were treated in sodium alginate (control) and sodium More
      In this study was conducted to investigate the effect of sodium alginate and cinnamon essential oils on shelf life of fillets of silver carp (Hypophthalmichthys molitrix) fillets in refrigerated storage. Fish fillets were treated in sodium alginate (control) and sodium alginate treatments containing 0.5, 1 and 1.5% cinnamon oil in order to achieve the best percentage of oil and each with 3 replicates were prepared. Chemical (TBA, TVB-N, pH, PV), microbial (TVC) factors and organoleptic characteristics at 0, 5, 10, 15, 20 day were evaluated. Results showed that with increasing storage time, the pH of the treatments studied had no significant difference (P> 0.05).In all treatments, PV value from the limited level (10-20 mEq/ kg lipid) not higher. Changes of TBA in 1.5% essential oil of cinnamon coated fillet were less than other treatments (P< 0.05). In all treatments, TVB-N values was showed significant different (P< 0.05) on the 9th, 15th and 20th. TVC counts were not higher than 7 log cfu/ g in 1 and 1.5 % treatments of essential oil. Sensory characteristics of treatments were not acceptable on 20th day. The results showed that sodium alginate coating contains essential oils of cinnamon in silver carp fillets during refrigerated storage at 1.5 % cinnamon essential oil treatments was 15 days. Manuscript profile
    • Open Access Article

      2 - Antimicrobial activity of grass kelp macroalgae (Ulva intestinalis) hydro-alcoholic extract on cultured Beluga (Huso huso) minced fish during refrigerated storage
      Mitra Daneshvar Ghorbani سید پژمان حسینی شکرابی Seyed Ebrahim Hosseini
      This study was carried out to investigate the possibility of using Ulva intestinalis macroalgae extract as a natural antibacterial agent in Beluga minced meat. For this purpose, the minimum of inhibitory concentration (MIC) of the hydroalcoholic extract of the algae was More
      This study was carried out to investigate the possibility of using Ulva intestinalis macroalgae extract as a natural antibacterial agent in Beluga minced meat. For this purpose, the minimum of inhibitory concentration (MIC) of the hydroalcoholic extract of the algae was determined in vitro by micro-well dilution method against Listeria monocytogenes pathogen. Then, the concentrations of controls (without any additive), BHT (as a synthetic antioxidant), MIC, 2 fold of the MIC (2MIC) and 3 fold of the MIC (3MIC) concentrations, were mixed with minced meat and refrigerated. Microbial properties of the samples including psychrophilic bacteria count, mesophilic bacteria count, Pseudomonas count and Enterobacteriaceae count were measured at 0, 4, 8, and 12 days. According to the results, the MIC was 12.5 mg/mL. The result of this study showed that the samples with the algae extract had less microbial load in comparison with control and BHT groups (p <0.01). Also, 3MIC showed the most antibacterial effect among other algae extract treatments by the lowest amount of mesophilic bacteria (6.27 Log cfu/g), cold (09.08 Log cfu/g) Pseudomonas (6.6 Log cfu/g) and Enterobacteriaceae (6.21 Log cfu/g) population size at the end of storage period. The results indicated that grass kelp macroalgae is a suitable species for use as a natural preservative, and the minced fish with three fold of the MIC concentration had less bacterial spoilage during the storage period in a refrigerator. Manuscript profile
    • Open Access Article

      3 - The effect of shallot on probiotic bacteria viability and some physicochemical and overall acceptability of low-fat stirred yogurt
      Rokhsareh Ramezani Vajiheh Fadaei Noghani Hasan Judaki
      Since most probiotic foods are dairy products and with regard to changing taste of people, dairy product manufacturers are trying to develop new products, which have high nutritional value in addition provide variety for consumer‘s taste. Nowadays, flavored yogurt More
      Since most probiotic foods are dairy products and with regard to changing taste of people, dairy product manufacturers are trying to develop new products, which have high nutritional value in addition provide variety for consumer‘s taste. Nowadays, flavored yogurt has a wide adoption between consumers. Flavoring of yogurt is made by adding natural ingredients such as a variety of fresh or dried fruits and vegetables such as mint, pennyroyal, basil and shallot. In this study, the effects of adding Iranian shallot at 0/2, 0/3 and 0/4% on the viability of probiotic bacteria (Lactobacillus acidophilus and Bifidobacterium lactis) and some physico-chemical (pH, titratable acidity, syneresis and viscosity) and overall acceptability of low-fat stirred probiotic yogurt containing 0/08 percent beta-glucan was investigated. The results showed that the addition of shallot affected significantly titratable acidity and pH of yogurt samples (p<0.05). With increasing the percentage of shallot, the viscosity and the viability of probiotic bacteria of stirred yoghurt samples was decreased and syneresis and overall acceptability was increased (p<0.05). Manuscript profile
    • Open Access Article

      4 - Prevalence and phylogenetic analysis of Listeria monocytogenes isolated from the fillets of two farmed fish in Shahrekord in 2018
      Esmaeil Pirali Khairabadi Seyedh Sedigheh Mousavi Hassan Momtaz Farzaneh Nikokhah Seyed Pejman Hosseini Shekarabi Mehdi Raissy
      Fish and other aquatic animals, directly and indirectly, can be contaminated with Listeria monocytogenes and consequently, fish and their products are considered a carrier for this bacterium. The present study investigated the contamination of farmed rainbow t More
      Fish and other aquatic animals, directly and indirectly, can be contaminated with Listeria monocytogenes and consequently, fish and their products are considered a carrier for this bacterium. The present study investigated the contamination of farmed rainbow trout and common carp with this bacterium as well as the phylogenetics of the isolates. A total of 50 samples of farmed fish were obtained from local fish supply centers in Shahrekord City from July to September 2018. Based on the molecular experiments, 18 (36%) of the 50 studied samples were contaminated with L. monocytogenes as 26% and 10% of the isolates were found in trout and carp, respectively. From 18 isolates, 12 samples (66.66%) belonged to serotype 4b, one belonged to serotype 1/2a and the other 5 samples belonged to serotype 1/2b. According to the results of the present study and regarding the importance of the bacteria in terms of public health, continuous and accurate hygienic monitoring seems to be necessary. Manuscript profile
    • Open Access Article

      5 - The effect of gamma rays on shelf life of grass carp (Ctenopharyngodon idella) fillet in the refrigerator condition
      reza shahhoseini Zohreh Mashak
      Various ways to increase the duration used for food storage, among whom radiation can be used as an effective way to retard spoilage in food in particular is concerned with the origin of the fish. In this study 50 grass carp (Grass carp) (Ctenopharyngodon idella) (mean More
      Various ways to increase the duration used for food storage, among whom radiation can be used as an effective way to retard spoilage in food in particular is concerned with the origin of the fish. In this study 50 grass carp (Grass carp) (Ctenopharyngodon idella) (mean weight 1-1.5 kg) for gamma irradiation with cobalt-60 source by doses of zero, 1.5, 2.5 and 3.5 kGy chosen and then change microbial load and total volatile nitrogen in the days 1, 7 and 14 were evaluated under the conditions of storage in refrigerator. These research results demonstrate the significant decline in aerobic mesophilic bacteria and T.V.N in the irradiated samples in comparison with non-irradiated samples. In this study, given that the dose of 3.5 kGy dose caused a significant decrease in comparison with other (standard range) T.V.N values and aerobic mesophilic bacteria was in the refrigerator until the seventh day. Therefore, in Grass carp using it (3.5kGy) as the best dose to prevent corruption is recommended in seventh days. Manuscript profile
    • Open Access Article

      6 - Review on health risk of Vibrio in seafood
      Mehdi Raissy Roza Fatahi Parto Reisi
      Seafood has found its way into eating habits of consumers. However, it can be accompanied with some hazards such as transmission of pathogenic bacteria. The origin of disease can be either a fish pathogen or a secondary contamination. Vibrios are of the important bacter More
      Seafood has found its way into eating habits of consumers. However, it can be accompanied with some hazards such as transmission of pathogenic bacteria. The origin of disease can be either a fish pathogen or a secondary contamination. Vibrios are of the important bacteria which are naturally found in the environment and in the aquatic animals' body as microflora and results in vibriosis if ingested raw or undercooked. The most important species are Vibrio parahaemolyticus, V. Cholera, V. vulnificus and V. alginolyticus. Although vibrio species are distributed worldwide, but it seems that they are more prevalent in some areas due to nutritional habits. This article reviews important species, source of infection and the previous reports. Manuscript profile
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      7 - The study of probiotic Lactobacillus plantarum on slime- producing Staphylococcus aureus
      Nazila Arbab Soleimani Maryam Mahdavi
      10.30495/jfm.2023.1972043.1797
      The present study was conducted with the aim of evaluating the antibiotic resistance pattern of Bacillus cereus in ice cream, cake, cheese, and infant formula. For this purpose, 600 samples were prepared from food sales centers in Tehran and transferred to the quality c More
      The present study was conducted with the aim of evaluating the antibiotic resistance pattern of Bacillus cereus in ice cream, cake, cheese, and infant formula. For this purpose, 600 samples were prepared from food sales centers in Tehran and transferred to the quality control laboratory of the Faculty of Veterinary Medicine of Tehran University of Medical Sciences and cultured on the special culture medium of Bacillus cereus, MYP, which contained polymyxin B supplement and egg yolk. Then, from the pink colonies, gram staining and catalase test were performed and the sensitivity of the isolated bacteria to ampicillin, penicillin, vancomycin, cefazolin, methicillin, cefixime, sulfamethoxazole, trimethoprim, chloramphenicol, cefepime and tetracycline was evaluated based on the disc diffusion method. According to the results, no contamination with B. cereus was observed in any of the cake, cheese and ice cream samples. However, out of 150 infant formula samples, 30 samples were infected with B. cereus (18 samples < 10 CFU/g, 7 samples had 10-102 CFU/g, and 5 samples>102 CFU/g). B. cereus isolated from infant formula has the highest sensitivity to vancomycin (67.76%), tetracycline (70%), and chloramphenicol (33.63%) antibiotics and had the highest level of resistance to penicillin (100%), cefixime antibiotics (100%) and ampicillin (67.96%). The general results indicated a significant prevalence of B. cereus in the tested milk, which due to the growing trend of using milk powder and more importance to the quality and health evaluation of milk powders for consumption, contamination investigation and preventive approaches should be given more attention. Manuscript profile
    • Open Access Article

      8 - Bacterial zoonotic disease from fish: a review
      Mehdi Raissy
      Zoonoses involving transmission of disease and biotoxin producing agents from fish to humans have been documented in the literature mostly including bacteria, biotoxins and parasites. The list of potential fish-borne zoonoses is quite large. Twenty six infectious agents More
      Zoonoses involving transmission of disease and biotoxin producing agents from fish to humans have been documented in the literature mostly including bacteria, biotoxins and parasites. The list of potential fish-borne zoonoses is quite large. Twenty six infectious agents are discussed in this review and there are also many other infectious organisms of fish origin that have not been reported but have the potential to infect and harm human. The status of the human host immune system plays a vital role in the severity of the disease. The major exposure routes include ingestion and introduction of organisms through open wounds or abrasions. More specifically, ingestion includes consumption of raw or under-cooked infected fish tissue, ingestion of fish tissue contaminated with feces from infected fish. Overall 46.15 % of fish-borne zoonoses are transmitted orally which are mostly helminthic diseases are caused by Trematodes, Cestodes and nematodes. Yet these zoonoses are responsible for large numbers of human infections around the world. Ingestion of water harboring infectious organisms with 23.07 % and Dermal exposure includes introduction of infectious agents into open wounds or abrasions through handling infected fish or infected water with 19.23 % are other transmission ways and importantly 15.38 % of fish-borne zoonoses are transmitted by more than one way. Distribution, epidemiology, clinical aspects, and the research needed for improved risk assessments, clinical management and prevention and control of these important diseases are reviewed. Manuscript profile
    • Open Access Article

      9 - Study of chemical, microbial indicators and shelf life of consolidated burgur (silver carp-hen) during cold storage
      Laleh Romiani Shiva Alizadeh
      This Study aimed to investigate the indicators spoilage chemical and shelf life of consolidated burger (silver carp- hen) during 6 months of storage at -18 ºC. For this purpose, fish and hen samples with 5 treatments: 1(75% fish+without pen), 2(without fish+75% pen More
      This Study aimed to investigate the indicators spoilage chemical and shelf life of consolidated burger (silver carp- hen) during 6 months of storage at -18 ºC. For this purpose, fish and hen samples with 5 treatments: 1(75% fish+without pen), 2(without fish+75% pen), 3(56.25% fish+18.75 pen), 4(18.75% fish+ 56.25% pen) and 5 (37.5% fish+37.5% pen) with 3 frequency were produced. The results showed quality deterioration indices were increased during storage. The most of PV (4.08±0.01 meq O2 kg-1), TVB-N (20.26±0.92 mg N2 100/g), TBA (3.63±0.06 mg Malonaldehyde/kg) and FFA (3.94±0.03%) were evaluated in the 1 treatment in sixth month. Comparing to the first month, the psychrophilic bacterial counts (PTC) and total viable count (TVC) values were significantly delayed in samples 4 and 5 treatments. Freezed silver carp burger stored at −18 °C retained very good quality, with respect to growth of PTC and TVC bacteria. Considering the results, the best shelf life of consolidated burger was fourth month in the 2 treatment. Manuscript profile
    • Open Access Article

      10 - A study on the antibacterial effects of ethanolic and methanolic extracts of Saffron (Crocus sativus L.) on some food borne pathogens
      Hossein Motamedi سید منصور سیدنژاد اسماعیل داراب پور
      Control of food borne diseases is a major concern and due undesirable side effects of chemical preservatives, there is more tendencies to natural preservatives such as spices for this purpose. Saffron is the most expensive spice that is is used as coloring and flavoring More
      Control of food borne diseases is a major concern and due undesirable side effects of chemical preservatives, there is more tendencies to natural preservatives such as spices for this purpose. Saffron is the most expensive spice that is is used as coloring and flavoring agent in foods. The aim of the present study was investigate antibacterial effects of hydro alcoholic extracts of saffron against pathogenic bacteria especially food poisoning species. For this purpose, one gram of finely powdered saffron was mixed with 10 ml of 2:8 (v/v) water- alcohol (ethanol or methanol) solution and mixed gently. Following incubation at room temperature, the suspension was centrifuged and the extract of saffron was harvested and dried. Sterile blank discs were saturated with extracts and their effects were evaluated in standard disc diffusion assay against six gram positive and six gram negative bacterial species. Simultaneously, the effect of standard antibiotics was evaluated against tested bacteria. The results revealed that Staphylococcus aureus, Bacillus anthracis, Bacillus cereus, Listeria monocytogenes and Brucella melitensis were the most susceptible species to ethanolic and methanolic extracts of saffron while Proteus mirabilis and Salmonella Typhi were resistant. The antibacterial effects of extracts were noticeable with regard to the standard antibiotics. All of the susceptible species in this study are important food borne pathogens and it is possible to use saffron as a natural preservative in food industry in order to control food borne pathogens. Manuscript profile
    • Open Access Article

      11 - Production of probiotic juice by use of two varieties of Red and Yellow apple
      neda Ghazavi Hamdollah Moshtaghi Mojtaba Bonyadian
      Probiotic bacteria due to having positive effects on health, widely used in the manufacture of foods, particularly in the production of dairy products, but having lactose intolerance problem and high cholesterol of dairy products is an important restrictions on the use More
      Probiotic bacteria due to having positive effects on health, widely used in the manufacture of foods, particularly in the production of dairy products, but having lactose intolerance problem and high cholesterol of dairy products is an important restrictions on the use of them. Recently, the consumption of non-dairy probiotic foods and specifically non-dairy probiotic juices were developed. The aim of this study was to examine the growth trend of Lactobacillus acidophilus in the two types of red and yellow apple. After inoculation of bacteria to each sample, they were stored at 4°C and at certain times during fermentation, changes in pH, titratable acidity and the sediment, as well as live cell counts were measured under controlled conditions. Both treatments showed a significant decrease in pH, sediment and bacteria population at the end of maintenance, but the changes in acidity were not significant (p<0.05). Sensory evaluation of apple juice on the fifth day showed no significant difference between the control sample and probiotics. On the tenth day of sensory evaluation the probiotic juice significantly has higher concentration than the control sample. In general, red apple juice in compared with yellow apple juice has more favorable conditions for growth of Lactobacillus acidophilus. There was no desirable effect on sensory properties of apple juice after adding probiotics.                                                                                                      Manuscript profile
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      12 - Cloning and sequencing 5' and 3' SipA gene of Salmonella enteritidis in E.coli
      Gazizeh Shirazi Abbas Doosti
      Introduction: Salmonella infections in many domesticated animals, wildlife and humans cause disease all of the world. The ability to enter and survive in host cells is a prerequisite for pathogenicity species of Salmonella. Salmonella invasion protein is an important vi More
      Introduction: Salmonella infections in many domesticated animals, wildlife and humans cause disease all of the world. The ability to enter and survive in host cells is a prerequisite for pathogenicity species of Salmonella. Salmonella invasion protein is an important virulence factors into host cells by bacteria transmitted. In this study we showed cloning the 5 'and 3' gene SipA Salmonella and was performed in E. coli. Materials & Methods: In this study, by PCR and specific primers sequences of 5' and 3' SipA gene was amplified correctly. DNA fragment was cloned by T/A cloning technique in pGEM T-easy vector and transformed into E. coli. Results: Cloning of SipA gene was confirmed by PCR. The results of next step showed that the SipA gene was successfully cloned in E. coli. Final confirmation of construct was done by XbaI, BglII, SacI, XhoI restriction enzymes. Conclusion: According to the results that, insertion of antibiotic-resistant genes between 5' and 3' regions of the SipA gene of Salmonella entritidis due to gene deletion in the bacteria. The construct's gene can be used as a target for gene vaccines against Salmonella entritidis in the future. Manuscript profile
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      13 - Antimicrobial and antioxidant properties of ascorbic acid on common carp (Cyprinus carpio) fillet during refrigerated storage
      فریبرز قجقی سید پژمان حسینی شکرابی آتوسا محمدی
      In this study the effect of preliminary ascorbic acid soaking treatments with two concentration 100 and 300 mg on the microbial growth and rancidity development in common carp fillet during refrigerated storage (4±1ºC) up to 21 days was investigated. Microbi More
      In this study the effect of preliminary ascorbic acid soaking treatments with two concentration 100 and 300 mg on the microbial growth and rancidity development in common carp fillet during refrigerated storage (4±1ºC) up to 21 days was investigated. Microbial analysis including total viable counts and psychrophilic bacteria counts as well as the peroxide, total volatile basic-nitrogen, free fatty acids, thiobarbituric acid and pH were periodically measured every 7 days during storage period. According to the results, the treated sample with 300 mg ascorbic acid significantly reduced the total viable counts as well as psychrotrophic bacteria counts in comparison to other groups (p<0.05). The maximum and minimum amounts of peroxide value was calculated in the control (6.113±0.471 meq/kg) and 300 mg of ascorbic acid (3.933±0.045 meq/kg), respectively (p<0.05). The amount of total volatile base nitrogen was controlled just in 300 mg of ascorbic acid treatment than other treatments at the end of storage day (p<0.05). Increasing trend of thiobarbituric acid was  significantly inhibited in 300 mg ascorbic acid treatment compared to control group during storage (p<0.05). The maximum value of free fatty acids (FFA) was obtained in the control (18.378±0.966%) (p<0.05), while the amount of  FFA in 100 and 300 mg of ascorbic acid treatments were reached 9.967±0.911%  and 7.017±0.267%, respectively. Results of bacterial and chemical analyses showed that bacterial growth and formation of primary and secondary lipid oxidation compounds were controlled by increasing ascorbic acid concentration which was corroborated by a longer shelf-life time. Manuscript profile
    • Open Access Article

      14 - Evaluation of antibacterial effects of some plant extracts and essential oils against Vibrio parahaemolyticus
      Mehdi Raissy Ali Daghaghelelh Mojtaba Alishahi Mehdi Rahimi
      In this study the antibacterial effects of ethanolic extract of eight medicinal plants (Artemisia aucheri, Satureja bachtiarica, Tanacetum parthenium, Cuminum cyminum, Salvia officinalis, Achillea kellalensis, Stachys lavandulifolia, Myrtuscommunis) and the essential oi More
      In this study the antibacterial effects of ethanolic extract of eight medicinal plants (Artemisia aucheri, Satureja bachtiarica, Tanacetum parthenium, Cuminum cyminum, Salvia officinalis, Achillea kellalensis, Stachys lavandulifolia, Myrtuscommunis) and the essential oil of Satureja bachtiarica, Artemisia aucheri, Tanacetum parthenium, Cuminum cyminum, Achillea kellalensis, Myrtuscommunis on Vibrio parahaemolyticus were evaluated. Standard tube dilution method was used for evaluation of Minimum Bactericidal Concentration value (MBC) and Minimum Inhibitory Concentration value (MIC) of each plant. Then inhibitory zone of bacterial growth for each plant was also measured and compared to positive and negative control. The results showed that the extracts of Satureja bachtiarica had the highest antibacterial effects on V. parahaemolyticus with the inhibitory bacterial growth zone of 15 mm. The extract of Tanacetum parthenium and Cuminum cyminum were also effective with the inhibitory bacterial growth zone of 14 and 11.67 mm against V. parahaemolyticus. Other herbal extract showed no antibacterial effects. The results revealed the higher antibacterial effects of essential oils comparing to the extracts and the highest antibacterial effect were belonged to Satureja bachtiarica, Tanacetum partheniumand Cuminum cyminum. It can be concluded that regarding to high antibacterial effect of Satureja bachtiarica, Tanacetum parthenium and Cuminum cyminum, they can be used in elimination of V. parahaemolyticus. Manuscript profile
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      15 - Detection of zoonotic Lactococcus garvieae in rainbow trout meat and evaluation of virulence gene
      Mehdi Raissy mahsa Ansari
      Lactococcosis which is caused by Lactococcus garvieae is now widespread. This species is categorized in zoonosis agents and is able to cause endocarditis especially in immunosuppressed persons. This study was done to detect Lactococcus garvieae in cultured rainbow trout More
      Lactococcosis which is caused by Lactococcus garvieae is now widespread. This species is categorized in zoonosis agents and is able to cause endocarditis especially in immunosuppressed persons. This study was done to detect Lactococcus garvieae in cultured rainbow trout meat collected from retail market and to evaluate the virulence of the isolates. A total of 90 fresh fish were randomly selected and were transferred to the laboratory in appropriate conditions. After collecting meat samples, the bacteria were investigated by culture on BHIA and standard biochemical tests. The results were confirmed by PCR. Presence of virulence gene in the isolates were studied and the results revealed that 4 isolates of a total of 9 collected isolates harbored cap virulence gene (44.4%). According to the results, rainbow trout meat can be contaminated with Lactococcus garvieae which is a potential danger for consumers. However, it depends on the nutritional habits and methods of reservation. Manuscript profile
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      16 - A Study on the Synergistic Effect of Sunflower Oil Based Nanoemulsion with Zataria multiflora Boiss. Essential Oil on the Quality of Rainbow Trout Fillets
      Shabnam Shadman Sayyed Ebrahim Hosseini Hadi Ershad Langroudi Shahrokh Shabani
      The quality changes of farmed rainbow trout (Oncorhynchus mykiss) fillets treated withsunflower oil based nanoemulsion with or without Zataria multiflora Boiss. essential oil was studied on days 0, 5, 10 and 15 during storage in (4oC±1) and evaluated by microbiol More
      The quality changes of farmed rainbow trout (Oncorhynchus mykiss) fillets treated withsunflower oil based nanoemulsion with or without Zataria multiflora Boiss. essential oil was studied on days 0, 5, 10 and 15 during storage in (4oC±1) and evaluated by microbiological and sensory analyses. Total viable counts and psychrophilic bacteria were determined. The results showed that the nanoemulsion had significant effect (P<0.05) on total viable counts. No growth of psychrophilic bacteria was found. Sensory analysis showed that the nanoemulsion could improve colour features. Odour and texture were enhanced as well. Incorporation of Z. multiflora essential oil and the nanoemulsion increased the acceptability for taste, odoure and texture. The inhibitory effects of the nanoemulsion were closely related to the concentration of the essential oil due to high content of carvacrol and thymol. The quality of fillets treated with nanoemulsion and 1% Z. multiflora essential oil was significantly different compared with other treatments and had acceptable results until day 15 of storage. Therefore, the nanoemulsion developed in this study could be represented as an option as a food preservative. Z. multiflora Boiss. essential oil showed a synergistic effect with the nanoemulsion for shelf-life extension of rainbow trout fillets.  Manuscript profile
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      17 - Effect of Lactic acid on microbiological and chemical indices of common carp fillets during storage at 4 0C
      سید محمد علی نوری سعید خانزادی علی فضل آرا حسین نجفزاده ورزی محمد عزیززاده
      This study was carried out to evaluate shelf life, microbiological and chemical attributes of common carp (Cyprinus carpio) fillets treated by dipping in 1.5% lactic acid solution during refrigerated storage at 4 °C for 18 days. Mesophilic and psychrophilic counts, More
      This study was carried out to evaluate shelf life, microbiological and chemical attributes of common carp (Cyprinus carpio) fillets treated by dipping in 1.5% lactic acid solution during refrigerated storage at 4 °C for 18 days. Mesophilic and psychrophilic counts, TVN (Total Volatile Nitrogen), pH, TBA and Expressible Moisture (EM) were carried out at 0, 3, 6, 9, 12, 15 and 18 days of storage. The microbiological and chemical analyses demonstrated significant reduction in mesophilic and psychrophilic counts, TVN and pH in treatment group (p Manuscript profile
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      18 - Changes of the sensory attributes and microbial load of grass-carp (Ctenopharyngodon idella) fillet affected by thermal drying process during storage at 4°C
      Masoud Hedayatifard Nahmeh Youseftabar-Miri Abolfazl Fadavi
      The sensory attributes (including color, odor, texture, taste and general acceptability) and microbial communities load (including TVC and number of mold-yeast) of Grass carp were investigated as affected by thermal drying. The samples were dried within 4-24 hrs in a la More
      The sensory attributes (including color, odor, texture, taste and general acceptability) and microbial communities load (including TVC and number of mold-yeast) of Grass carp were investigated as affected by thermal drying. The samples were dried within 4-24 hrs in a laboratory dryer at 65°C and were packed under air condition and stored at 4°C. Temperature and time were recorded until moisture reduced to 35%. Both Row and dried samples were analyzed. The results showed that there were no difference in sensory attributes and microbal counts after drying processs (p>0.05). The results also showed that all of microbial counts and sensory attributes were changed during cold-storing. Microbial load of packed just-dried fish was lower than cold-stored samples; qua bacteria and mold-yeast counts were significantly increased at the day 30 from 2.90 to 3.58 and 1.62 to 3.31 Log cfu/g, respectively (p<0.05). Among of organoleptic parameters, taste and texture showed most changes during storing (p<0.05). Finally, dried products although showed a Quality loss in sensory and microbial indices, had an acceptable quality during 30 days of storage. Manuscript profile
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      19 - Investigating effect of pH and concentration of strawberry juice on the viability of Lactobacillus casei and Lactobacillus plantarum
      Tahereh Berimavandi Vajiheh Fadaei Noghani
      In this studyresearch, probiotic strawberry juice was produced using probiotic bacteria Lactobacillus casei and Lactobacillus plantarum and four factors brix (9,11,13), pH (3,4), time (0,7,14,21,28) and bacteria (Lactobacillus casei and Lactobacillus plantarum) were stu More
      In this studyresearch, probiotic strawberry juice was produced using probiotic bacteria Lactobacillus casei and Lactobacillus plantarum and four factors brix (9,11,13), pH (3,4), time (0,7,14,21,28) and bacteria (Lactobacillus casei and Lactobacillus plantarum) were studied using factorial design in a completely randomized factorial design; and some physicochemical properties (pH, acidity, vitamin C and soluble solids) and probiotic bacterial population of probiotic strawberry juice were measured. The result showed that during storage time, pH, vitamin C, total soluble solids and bacterial population decreased (p<0.05) and acidity increased (p<0.05). With increasing the brix, acidity, soluble solids and bacterial population increased (p<0.05) and vitamin C decreased (p<0.05). With increasing pH, Vitamin C and bacterial population increased (p<0.05) and acidity decreased (p<0.05). The results showed Lactobacillus casei was higher; also, the highest viability of probiotic bacteria in pH = 4, brix= 13 and storage time zero was observed. Keywords: Strawberry, Lactobacillus casei, Lactobacillus plantarum, probiotic juice Manuscript profile
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      20 - The effect of application of bioactive packing cover on the basis of natural preservative (chitosan / natamycin) and microalgae, Spirolina platensis and Chlorella vulgaris on Quality Characteristics and Beef Shelf Life
      reza shafiei toktam mostaghim
      The aim of this study was to investigate the effects of antimicrobial coating based on chitosan, and natamycin, Chlorella vulgaris, Spirolina platensis for increasing the survival rate of beef. For this purpose, the values of zero, 1, 1.5 and 2 percent of the compounds More
      The aim of this study was to investigate the effects of antimicrobial coating based on chitosan, and natamycin, Chlorella vulgaris, Spirolina platensis for increasing the survival rate of beef. For this purpose, the values of zero, 1, 1.5 and 2 percent of the compounds were used during the days of zero, 7, 14 and 28 days storage in the packaging. Physicochemical meat tests including pH, ash percentage, protein, volatile nitrogen, index of thiobarbituric acid, texture index (Hardness, elasticity, cohesively, consistency, chewiness), total microbial population, sensory evaluation (crispiness, taste, wateriness, overall acceptance) in a completely randomized design with three repetitions at a significant level of 95% confidence has been checked. The results of the experiments were analyzed by using Minitab 17.2 software. The results showed that there were significant differences between the calf meat treatment Physicochemical and sensory with the testator treatment and increasing the use of chitosan, algae and also kanamycin, increase of microbial index rate, volatile nitrogen index (TVBN), thiobarbituric index (TB) during storage time for 28 days will be happened by less intensity. But the percentage of ash index did not show significant differences (p<0.05). The use of algae in packaging formulations increases by up to 1%, increased hardness, decreased elasticity, chewiness and cohesiveness, as well as gumminess. With the increasing in the use of chitosan and the prevention of microbial and antioxidant damage as well as the use of algae due to its antioxidant properties, the rheological properties of the tissue as well as sensory evaluation were significantly less than the control treatment. Finally, taking into account the Physicochemical properties, the sensory packaging coverage by using 1% platinaceous algae, Chlorella vulgaris1% ,Spirolina platensis,1% chitosan and 1% natamycin was used as the best composition in order to cover packaging. Manuscript profile
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      21 - Effect of nisin on microbial quality of farmed great sturgeon Huso huso in refrigerator and freezing temperatures
      محمدرضا قمی مسعود Ghane مینا Eghbali مهدی Nikoo
      Effect of nisin on microbial quality of farmed great sturgeon Huso huso in refrigerator and freezing temperatures In this study the effects of nisin (a biotic preservative) (0, 0.2 and 0.4%) on the microbiological quality of farmed great sturgeon (H. huso) slices inocu More
      Effect of nisin on microbial quality of farmed great sturgeon Huso huso in refrigerator and freezing temperatures In this study the effects of nisin (a biotic preservative) (0, 0.2 and 0.4%) on the microbiological quality of farmed great sturgeon (H. huso) slices inoculated by Staphylococcus aureus and Escherichia coli (8 log cfu/ml) during storage (4 and -18 °C) for 10 days was determined. The counts of S. aureus did not change significantly after ten days of storage (P> 0.05) and in other words, it was not affected from different concentrations of nisin and storage temperatures. Frozen samples treated by nisin showed lower counts of E. coli. Nisin at both concentrations had the same effects on reducing the counts of both bacteria. The positive effects of such a low-risk biological preservative in studied food product appearing to reduce the microbial load, therefor its usage in preservation of food is industrially preferable rather than other chemical preservatives. Keywords: Nisin, Storage temperature, Staphylococcus aureus, Escherichia coli, Farmed beluga. Manuscript profile
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      22 - The Effect of Using Native Carbon Sources (Whey, Oat bran, Rice Flour) in the Fermentative Production of Lysine by Corynebacterium glutamicum
      Zahra Mohammadzade Ferizhandi Ghazal Labbeiki
      Lysine is an essential amino acid that is not synthesized in the human body, but due to the body's need and its application in different parts of the health area, it is important to optimize and economize the production process. The most commonly used lysine production More
      Lysine is an essential amino acid that is not synthesized in the human body, but due to the body's need and its application in different parts of the health area, it is important to optimize and economize the production process. The most commonly used lysine production method is microbial fermentation and the nutrient composition of the fermentation media is effective on the microorganism growth and the production of microbial metabolite. Accordingly, the aim of this study was to investigate the effect of native and inexpensive nutritional substrates, as the main supplier of energy for microorganism, on the growth and productivity of strains. For this purpose, different amounts (g/lit) of whey (100, 135, 170), rice flour (100, 135,170) and oat bran (100, 170, 220) were separately added to culture of Corynebacterium glutamicum, and at 68 and 116 fermentation time, growth indices such as pH of the environment, production biomass, specific growth rate of microorganism, strain morphology and amount of produced amino acid were measured and compared with control (100 g/lit glucose). The results showed that the highest amount of lysine produced was obtained in media containing 135 g/lit whey, 170 g/lit rice flour and 220 g/lit oat bran. These results indicate that these compounds are suitable for use in biological processes and can be used as the main nutrient source. Manuscript profile
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      23 - Determine the effect of an increase in the general level of prices on food health: a retrospective study
      mahmoud Bakhshinejad
      The purpose of this study was to investigate the effect of monetary shocks and food prices on food health. And it's important and necessary in many ways. The most important is food and nutrition security in Iran, food and nutrition security is achieved when all people A More
      The purpose of this study was to investigate the effect of monetary shocks and food prices on food health. And it's important and necessary in many ways. The most important is food and nutrition security in Iran, food and nutrition security is achieved when all people At all times, the quantity and quality of the various foods with safe food content will be used to meet the needs and have a healthy and active life with health, a healthy environment and adequate care. Required data are extracted from the Bank of the Islamic Republic of Iran Economic Time Database and the Food and Drug Administration of the Ministry of Health and Medical Education during the period of 1975-96. The model used in the Cargo (2005) model was taken. This model is an error correction model with food contamination variables, real food prices, real per capita income, real exchange rate, and negative and positive money supply shocks. The results indicate that in the long run, positive and negative monetary shocks have a positive and negative effect on food micro-nutrients, as well as actual per capita income has a significant and positive effect on food microbial contamination in Iran.The purpose of this paper is to investigate the effect of monetary shocks and food prices on food microbial contamination, and it is important and necessary in many ways, most important of which is food and nutrition security in Iran, food and nutrition security is achieved when all people At all times, the quantity and quality of the various foods with safe food content will be used to meet the needs and have a healthy and active life with health, a healthy environment and adequate care. Required data are extracted from the Bank of the Islamic Republic of Iran Economic Time Database and the Food and Drug Administration of the Ministry of Health and Medical Education during the period of 1975-96. The model used in the Cargo (2005) model was taken. This model is an error correction model with food contamination variables, real food prices, real per capita income, real exchange rate, and negative and positive money supply shocks. The results indicate that in the long run, positive and negative monetary shocks have a positive and negative effect on food micro-nutrients, as well as actual per capita income has a significant and positive effect on food microbial contamination in Iran. Manuscript profile
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      24 - The effect of rice flour on survival of Lactobacillus paracasei in fruit yoghurt and its qualitative properties with response surface methodology
      Farnaz Nabizadeh Paniz Zinsaz
      In this study the effect of rice flour on survival of lactobacillus paracasei and qualitative properties of fruit yogurt was investigated during storage, using response surface method (RSM). For this, rice flour in three ranges of (0, 1.5 and 3%) was added to the milk. More
      In this study the effect of rice flour on survival of lactobacillus paracasei and qualitative properties of fruit yogurt was investigated during storage, using response surface method (RSM). For this, rice flour in three ranges of (0, 1.5 and 3%) was added to the milk. Then the milk and was pasteurized in 85ºC for 15 minutes. After cooling, yoghurt starter culture and probiotic bacterium inoculated in 43 ºC and added into sterile containers containing 15% peach puree. The samples were incubated in 42 ºC until reaching pH 4.6 ±0.1. Then the samples were cooled down to 4 ºC and refrigerated in the same temperature until experiment day. Survival of lactobacillus paracasei, pH, amount of moisture, acidity, syneresis and apparent viscosity and sensory properties were investigated in three periods of (1, 11 and 21 days). The statistical analysis of the results showed that with increasing of rice flour, lactobacillus paracasei count increased and decreased during storage (p Manuscript profile
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      25 - Occurrence and antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp caught from the Persian Gulf
      Neda Noroozi Hassan Momtaz Elahe Tajbakhsh
      10.30495/jfm.2022.1938722.1730
      The present study was performed to evaluate the occurrence and pattern of antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp samples caught from the Persian Gulf. A total of 240 seafood samples caught from the Persian Gulf, including 12 More
      The present study was performed to evaluate the occurrence and pattern of antibiotic resistance of Enterococcus faecalis strains isolated from fish and shrimp samples caught from the Persian Gulf. A total of 240 seafood samples caught from the Persian Gulf, including 120 fish and 120 shrimp samples were collected from Bushehr province. The presence of E. faecalis was confirmed using microbial culture and biochemical tests. The pattern of antibiotic resistance of E. faecalis isolates was evaluated using Disc Diffusion Method. Fifty-four out of 240 (22.50%) samples were contaminated with E. faecalis. The contamination of E. faecalis in fish and shrimp samples were 30% and 15 percent, respectively. E. faecalis strains isolated from fish and shrimp samples had the highest resistance to gentamicin (100%), tetracycline (100%), erythromycin (100%), cefazolin (90.74%) and trimethoprim-sulfamethoxazole (88.88%) antibiotics. Resistance to chloramphenicol was not observed in any of the isolates. The results of the study showed that fish and shrimp can be considered as possible sources of antibiotic-resistant E. faecalis. Therefore, complete cooking of seafood before consumption, observance of hygiene in fishing and sale centers and prescribing antibiotics according to the results of the disk diffusion test can prevent gastrointestinal infections caused by antibiotic-resistant strains of E. faecalis. Manuscript profile
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      26 - Molecular identification and evaluation of probiotic and antifungal properties of Entrococcus faecium isolated from natural honey
      Sara Shahryari Delasa Rahimi Hosein Purabdolah Maryam Ebrahimi Alireza Sadeghi
      10.30495/jfm.2023.1980405.1810
      Natural honey is a proper substrate to isolate probiotic bacteria. Characterization of probiotic and antifungal properties of microorganisms isolated from food ecosystems such as natural honey that have been less studied is an interesting task. In the present study, aft More
      Natural honey is a proper substrate to isolate probiotic bacteria. Characterization of probiotic and antifungal properties of microorganisms isolated from food ecosystems such as natural honey that have been less studied is an interesting task. In the present study, after molecular identification of a predominant lactic acid bacterium (LAB) isolated from the natural honey, its probiotic properties and antifungal effect were investigated. Then, the antibacterial (spot method) and antifungal (overlay method) activities of the LAB isolate were evaluated against some foodborne microorganisms. Sequencing results of the PCR products led to the identification of Enterococcus faecium. Survival rate of the isolate in simulated gastrointestinal conditions was equal to 20.68%, and its antibacterial and antifungal effects on Salmonella enterica and Aspergillus niger were also 31.06 and 32.35% inhibition, which were significantly (P<0.05) higher than the other foodborne indicators studied. Furthermore, the isolate had a proper antibiotic resistance profile, and it had no hemolytic activity. Auto-aggregation ability of the isolate was also equal to 32.34%, and its co-aggregation with Escherichia coli, S. enterica, Listeria monocytogenes and Bacillus cereus was 34.29, 21.35, 15.58 and 14.98%, respectively. Based on the results, it can be concluded that the E. faecium isolated from honey can be used as probiotic and/or protective culture in food industry. Manuscript profile
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      27 - شناسنامه علمی مجله
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      28 - shenaseh
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      29 - Summer 2022
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      30 - Winter 2020
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      31 - Summer 2021
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      32 - شناسنامه علمی مجله
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      33 - Scientific Information and Table of contents
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      34 - Editorial Board
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      35 - winter 2021
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      36 - Reviewers
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      37 - شناسنامه علمی مجله
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      38 - Spring 2022
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      39 - JFM_Volume 7_Issue 3_Pages 1-94
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      40 - SH
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      41 - Spring 2021
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      42 - Editorial Board
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      43 - table
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      44 - Scientific Information and Table of contents
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      45 - -
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      46 - List of Reviewers
      10.30495/jfm.2021.687162
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      47 - The names of the reviewers
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      48 - sh
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      49 - Spring 2019
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      50 - شناسنامه علمی شماره
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      51 - Spring 2019
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      52 - sh
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      53 - Winter 2019
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      54 - Summer 2019
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      55 - Fall 2019
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      56 - Scientific- Winter 1398
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      57 - JFM_Volume 7_Issue 2_Pages 1-91
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      58 - شناسنامه علمی شماره
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      59 - Editorial Board
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      60 - Table of Content
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      61 - Study of the changes in microbial load, putrescine and histamine in muscles of Otolithes ruber during storage in ice
      Ali Ghorbani Ranjbari Nazanin Ghorbani Ranjbari Alireza Golchin Manshadi Fatemeh Ghorbani Ranjbari
      Biogenic amines are small organic molecules formed by decarboxylase enzymes caused by bacteria in foodstuff. This study investigated the concentration of biogenic amines of putrescine and histamine in the fish stored in ice for an eighteen-day period in three day interv More
      Biogenic amines are small organic molecules formed by decarboxylase enzymes caused by bacteria in foodstuff. This study investigated the concentration of biogenic amines of putrescine and histamine in the fish stored in ice for an eighteen-day period in three day intervals using chromatography (days of 3, 6, 9, 15 and 18). In this study, the amine of histamine was not detected in the first and third day of storage; but putrescine was about 1.30±0.03 micrograms in the third day. The first day of detection of the both biogenic amines was the sixth day of storage. The primary concentration of histamine and putrescine was 1.51 and 1.30 micrograms per gram respectively, and was reach to 14.5 and 19 micrograms per gram respectively at the end of the period. Analysis of the results by ANOVA test indicated that there was a significant difference (P<0.05) in the concentrations of these amines between the first day (0) and the last day of storage. In addition, there was a significant relationship among the mesophilic bacterial load, increase of histamine, the psychrophilic bacterial load and the biogenic amine of putrescine (P<0.05). Manuscript profile
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      62 - Chemical and Microbial Quality of Pasteurized Yoghurt in the Zanjan Province in 2012 to 2014
      Adel Mirza Alizadeh Mehran Mohseni Abbas Ali Zamani Hamid Barani Bonab
      Abstract Generally, yogurt has the highest consumption among all fermented milk products in the world and has significant nutritional value, particularly protein and calcium. Yogurt due to having higher acid, often suffer micro-organism infections resistant to acid such More
      Abstract Generally, yogurt has the highest consumption among all fermented milk products in the world and has significant nutritional value, particularly protein and calcium. Yogurt due to having higher acid, often suffer micro-organism infections resistant to acid such as molds and yeasts. But main surface spoilage in yogurt occurs by mold. The present study was done between the years 2012 to 2014 on pasteurized yogurt samples collected from 9 dairy products industry of Zanjan Province for the purpose of monitoring the quality of products. The results showed that from among 50 samples of pasteurized yogurt, all of them in terms of acidity, pH and solids-not-fat (SNF) properties were consistent with national standards. Also in investigating the total bacteria count, no infection was observed with pathogenic micro-organisms. Findings from the study showed that most of the pasteurized yogurt produced in the Province of Zanjan in terms of the quality and biological characteristics is suitable and compliant with standards. Manuscript profile
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      63 - Study of the frequency of hlyAgene in Listeria monocytogenesisolated from Freshvegetables using PCR
      Hossein Momeni Ali Sharifzadeh Masoumeh Bashiri
      Abstract Listeria monocytogenesis an important psychotropic foodborne pathogen which may exist in fresh vegetables. Vegetables and their derivatives show a potential role in the transmission of Listeria to humans and epidemy of listeriosis.The objective of this study w More
      Abstract Listeria monocytogenesis an important psychotropic foodborne pathogen which may exist in fresh vegetables. Vegetables and their derivatives show a potential role in the transmission of Listeria to humans and epidemy of listeriosis.The objective of this study was to assess the prevalence of L. monocytogenesand to find out the frequency of hlyAgene in raw vegetables in Shahrekord, Iran. This cross-sectional study was carried out on 145 samples collected from vegetables market outlets in Shahrekord. Isolation of L. monocytogeneswas performed using specific medium. PCR was performed using hlyAgene specific primers. Also, all of the 145 samples were tested directly using PCR Totally, 56% of the samples were positive for Listeria in culture method. However, based on PCR, 56% out of 145 samples were infected to Listeria. Furthermore, hlyAgene was found in 67% of Listeria. The results indicate that the frequency of L. monocytogenesin the vegetablessamples of this region is relatively high. Manuscript profile
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      64 - Fall 2021
      10.30495/jfm.2021.686967
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      65 - Evaluation of the antioxidant and antibacterial effects of Apium petroseli-num essential oil on food spoilage and pathogenic bacteria
      Reza Sharafati Chaleshtori Mahmoud Rafieian-kopaei Ali Sharafati Chaleshtori Elham Salehi
      In this study, the total phenols and antioxidant activities of Apium petroselinum essential oil (APEO) were determined and its antibacterial effects on seven food spoilage and pathogenic bacteria studied in vitro. Having extracted the essential oil, the total pheno More
      In this study, the total phenols and antioxidant activities of Apium petroselinum essential oil (APEO) were determined and its antibacterial effects on seven food spoilage and pathogenic bacteria studied in vitro. Having extracted the essential oil, the total phenols were determined colorimetrically and the antioxidant activities evaluated by bleaching of β-Carotene. Its antibacterial effects in the form of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by Micro Dilution method against Alcaligenes faecalis, Providensia rettgeri, Serratia marcesens, Klebsiella oxitoka, Staphylococcus aureus, Shigella dysenteriae and Listeria monocytogenes. The results revealed that the amount of total phenols was 8.18±0.45 mg/g equivalent with Gallic acid and the antioxidant activities were 45±1.52 %. In addition, the APEO had antimicrobial effects on the above bacteria. The minimum inhibitory concentration (MIC) was between 1.562 and 12.5 and the minimum bactericidal concentration (MBC) was between 3.125 and 25 mg/ml. Therefore, APEO, because of having phenolic contents, enjoys antioxidant activities and antibacterial effects recommending as a suitable preservative in food industries. Manuscript profile
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      66 - Table of contents
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      67 - Determination of Aflatoxin M1 in Raw Milk Produced in Qazvin Province By ELISA and HPLC
      Reza Norian Ali Reza Pourfarzaneh Faramarz Mashatian
      Abstract Use of raw milk and dairy products containing aflatoxin M1 has led to concern in consumers. The aim of this study was to determine the aflatoxin M1 in raw cow milk in Qazvin Province. In this research, 170 raw cow milk were collected from dairy farm, dairy fac More
      Abstract Use of raw milk and dairy products containing aflatoxin M1 has led to concern in consumers. The aim of this study was to determine the aflatoxin M1 in raw cow milk in Qazvin Province. In this research, 170 raw cow milk were collected from dairy farm, dairy factories, milk collection centers and milk supply centers in Qazvin Province during winter 2013. The samples were analyzed with a commercial competitive enzyme-linked immune-sorbent assay (ELISA) kit and high-performance liquid chromatography (HPLC) for the samples with aflatoxin higher than 0.5 ng/ml. Statistical tests including ANOVA and Tukey were used to analyze the data. Aflatoxin M1 contamination was observed in 100% of the milk samples. Among the milk samples (33.52%) had a contamination of aflatoxin M1, higher than threshold level of The Institute of Standards & Industrial Research of Iran (0.5 ppb), while in 66.48%, concentration of aflatoxin M1 was less than the limits permitted. The mean concentration of aflatoxin in dairy farm was 0.215 ng/ml, in dairy factories was 0.268 ng/ml, in milk collection centers was 0.734 ng/ml and in milk supply centers was 0.409 ng/ml. Due to high level of contamination observed in samples, regular monitoring of production and distribution of milk and dairy products is necessary.  Manuscript profile
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      68 - A survey on bacterial contamination of fruit juice with Alicyclobacillus acidoterrestris using PCR and RFLP
      Hossein Motamedi Amir TajBakhsh
      Abstract Alicyclobacillus is an endospore forming and heat resistant bacterium that can cause changes in odor and color of fruit juice and make significant losses to juice industries. The purpose of this study was to identify this bacterium from different juic More
      Abstract Alicyclobacillus is an endospore forming and heat resistant bacterium that can cause changes in odor and color of fruit juice and make significant losses to juice industries. The purpose of this study was to identify this bacterium from different juices by PCR using specific primers. To do so, DNA extraction and PCR was done after culture and incubation. Of 24 tested samples, 5 cases were examined. As a consequence of enzymatic digestion with EcoR1 and electrophoresis, 118 and 528 bp fragments were obtained that are in accordance with RFLP pattern of Alicyclobacillus acidoterrestris. With regard to the obtained results, it can be claimed that contamination to this bacterium makes some problems in fruit juice industry. To diagnosis it better, PCR with high sensitivity can be used since it reduce the expenditures and save the time. Manuscript profile
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      69 - Evaluation of the antioxidant and antibacterial effects of Apium petroselinum essential oil on food spoilage and pathogenic
      Reza Sharafati Chaleshtori Mahmoud Rafieian Kopaei Ali Sharafati Chaleshtori Elham Salehi
        Abstract In this study, the total phenols and antioxidant activities of Apium petroselinum essential oil (APEO) were determined and it's antibacterial effects on seven food spoilage and pathogenic bacteria was in vitro studied. Having extracted the essential oil More
        Abstract In this study, the total phenols and antioxidant activities of Apium petroselinum essential oil (APEO) were determined and it's antibacterial effects on seven food spoilage and pathogenic bacteria was in vitro studied. Having extracted the essential oil, the total phenols were determined colorimetrically and the antioxidant activities evaluated by bleaching of β-Carotene. Its antibacterial effects in the form of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were determined by Micro Dilution method against Alcaligenes faecalis, Providensia rettgeri, Serratia marcesens, Klebsiella oxitoka, Staphylococcus aureus, Shigella dysenteriae and Listeria monocytogenes. The results revealed that the amount of total phenols was 8.18±0.45 mg/g equivalent with Gallic acid and the antioxidant activities were 45±1.52 %. In addition, the APEO had antimicrobial effects on the above bacteria. The minimum inhibitory concentration (MIC) was between 1.562 and 12.5 and the minimum bactericidal concentration (MBC) was between 3.125 and 25 mg/ml. Therefore, APEO, because of having phenolic contents, enjoys antioxidant activities and antibacterial effects recommending as a suitable preservative in food industries. Manuscript profile
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      70 - Antibacterial effect of Butylated Hydroxy Anisole (BHA) on Salmonella typhymurium and Listeria monocytogens in Culture media and Iranian White Cheese
      Ali Ehsani Mojtaba Raeisi Alijan Tabarraei Farhad Nik Nezhad Hossein Naghili Majid Aminzare
      The aim of this study is to evaluate antimicrobial effect of Butylated Hydroxy Anisole alone and in combination with EDTA against S. typhimurium and L. monocytogenes in culture media and Iranian white cheese. At first phase, Minimum Inhibitory Concentrations (MIC) of Bu More
      The aim of this study is to evaluate antimicrobial effect of Butylated Hydroxy Anisole alone and in combination with EDTA against S. typhimurium and L. monocytogenes in culture media and Iranian white cheese. At first phase, Minimum Inhibitory Concentrations (MIC) of Butylated Hydroxy Anisole and EDTA against bacteria were measured. The samples of cheese made from pasteurized milk where infected experimentally with S. typhimurium and L.monocytogenes. They were divided as a control and other samples that have values of 2500, 5000 and 10000 µg/ml of BHA, and combination of 2500, 5000 and 10000 µg/ml of BHA and EDTA. In the next phase of the study, bacteria were counted at different days. The results obtained from this study showed that using combination of BHA and EDTA can properly reduce number of  S.typhimurium and L. monocytogenes in culture media and Iranian white cheese.This study demonstrated that BHA can be used for increase shelf life of Iranian white cheese. Manuscript profile
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      71 - Isolation Alicyclobacillusacidoterrestris specific bacteriophage, the causative agent of fruit juice spoilage, from soil
      Amir TajBakhsh S.Elham Rezatofighi Hossein Motamedi Solmaz Rafiae
      Abstract Alicyclobacillusacidoterrestris is the main bacterial agent of fruit juice spoilage and the methods for its elimination are much paid attention to. Recently, using this phage for controlling bacteria in food products has received special attention. The aim of More
      Abstract Alicyclobacillusacidoterrestris is the main bacterial agent of fruit juice spoilage and the methods for its elimination are much paid attention to. Recently, using this phage for controlling bacteria in food products has received special attention. The aim of this study was to isolate the specific bacteriophages of A. acidoterrestris from soil. In this study, the samples collected from the soil around the Cedruslibani (Cedar) trees were investigated after being centrifuged and filtered to seek the understudied phage. Bacterial culture equal to0.5 McFarland turbidity, isolated from fruit juice and confirmed by molecular and biochemical techniques, was prepared in AAB. Adding a certain amount of environmental samples allowed the phage to be replicated in the environmentcontainingbacteria. Then, the environment was centrifuged and the supernatant, which is the source of the phage, was filtered. The pure phage suspension was assessed in double agar method for studying the formation, shape and size of plaques. Plaque formation confirmed the presence of bacteriophages in the filtrate. Consequently, the heat and acid resistant bacteriophage was isolated from the soilaround the Cedruslibani (Cedar) trees. The results revealed the presence of specific bacteriophage of A. acidoterrestris. This phage can be used for eliminating of this bacterium in fruit juice. Stability of these bacteriophages in fruit juices its specific. Preserving the fruit quality side is of advantages of using this bacteriophage comparing to other methods. Manuscript profile
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      72 - Detection of virulence factors in Salmonella typhimurium and Salmonella enteritidis serotypes isolated from chicken meat in Chaharmahal va Bakhtiari Province of Iran
      Hasan Momtaz Reza Ghaedamini Manouchehr Momeni
      Abstract Salmonellosis, caused by bacteria from the genus Salmonella, is one of the most common foodborne illnesses and is manifested by diarrhea, mild fever, nausea, abdominal pains and even death. Its traditional diagnosis is based on culture, biochemical and serologi More
      Abstract Salmonellosis, caused by bacteria from the genus Salmonella, is one of the most common foodborne illnesses and is manifested by diarrhea, mild fever, nausea, abdominal pains and even death. Its traditional diagnosis is based on culture, biochemical and serological techniques and for more accurate diagnosis several molecular methods have been developed. The epidemiology and prevalence of these bacteria in chicken meat samples is essentially unknown in Iran. in this study in order to isolation and characterization of Salmonella spp. 620 chicken meat samples were collected from supermarkets in Chaharmahal Va Bakhtiary Province, Iran. Genomic DNA was extracted from each sample and multiplex PCR method was developed. Results showed that 28 out of 620 samples (4.51%) were positive for Salmonella spp. and from these isolates 12 samples were S. typhimurium (1.93%), 10 samples were S. enteritidis (1.61%) and 6 samples were other species of Salmonella (0.96%). Results revealed that all of S. typhimurium and S. enteritidis isolates have virulence factors. Our results indicated that Salmonella spp. can contaminated chicken meat samples and it is essential to control the hygienic quality of chicken meat samples especially in slaughterhouse. Manuscript profile
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      73 - Study of the contamination of traditional cheeses produced in Maragheh city to Escherichia coli O157:H7
      Saman Mahdavi
      Abstract This study was conducted in villages of Maragheh city to determine the contamination of Escherichia coli O157:H7 in traditional cheeses. A total of 100 cheese samples were cultured in Tryptic soy broth containing cefixime and then they were transferred to Mac More
      Abstract This study was conducted in villages of Maragheh city to determine the contamination of Escherichia coli O157:H7 in traditional cheeses. A total of 100 cheese samples were cultured in Tryptic soy broth containing cefixime and then they were transferred to Mac conkey sorbitol agar containing cefixime and potassium tellurite. Suspicious colonies were stained (Gram staining) and identified by IMVC and other tests. Isolated Escherichia coli were tested for detection of O and H serotypes by direct agglutination method. Cheese samples were also tested for pH and NaCl content. Antibiogram test was done on Escherichia coli isolates by Kirby-bauer’s method. No Escherichia coli O157:H7was detected in the studied samples. In this study, other serotypes of Escherichia coli were isolated containing 32 non O157 serotypes. These serotypes including, Enteropathogenic (O55, O127, O86, O114, O44, O126, O125), Enterotoxigenic (O20, O128) and Verotoxigenic (O26, O111). All of the isolated strains were resistant to Ampicillin and Polymyxin B. The effect of prevalence ratio of Escherichia coli serotypes from pH and NaCl content was not significant statistically (p>0.05). From the results of this study it seems that traditional cheese could be a potent carrier to transmit the various serotype of Escherichia coli and cause disease in human. Manuscript profile
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      74 - Comparison of Nanosil and hydrogen peroxide in reducing of Escherchia coli population on egg shell surface
      Majid Gholami-Ahangaran Mohsen Jafarian-Dehkordi Mazyar Haj-salehi
      Abstract In this study the efficiency of two commercial anti-bacterial compounds comprising hydrogen peroxide and Nanosil D2 for reducing of bacterial population on egg shell surface was compared. Thus, 90 eggs were prepared and divided into 3 groups. In first group, Na More
      Abstract In this study the efficiency of two commercial anti-bacterial compounds comprising hydrogen peroxide and Nanosil D2 for reducing of bacterial population on egg shell surface was compared. Thus, 90 eggs were prepared and divided into 3 groups. In first group, Nanosil, in second group, hydrogen peroxide and in third group sterile water (as control) were sprayed on outer surface of egg shell. After 30 minutes, crushed egg shells were cultured. After preparation of serial dilution, Escherichia coli colonies were identified and counted according to usual bacteriological procedures. The results revealed there are no significant differences in amount of E. coli colonies between eggs exposed to Nanosil and hydrogen peroxide. The eggs exposed to Nanosil and hydrogen peroxide showed significant lower infectivity comparing to the control group. Thus, it seems that Nanosil can be used as a disinfectant of eggs. Manuscript profile
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      75 - Antibacterial effect of Butylated Hydroxy Anisole (BHA) on Salmonella typhymurium and Listeria monocytogens in culture media and Iranian White Cheese
      Ali Ehsani Mojtaba Raeisi Alijan Tabarraei Farhad Nik Nezhad Hossein Naghili Majid Aminzare
      Abstract The aim of this study was to evaluate antimicrobial effect of Butylated Hydroxy Anisole alone and in combination with EDTA against S. typhimurium and L. monocytogenes in culture media and Iranian White Cheese. At the first phase, Minimum Inhibitory Concentratio More
      Abstract The aim of this study was to evaluate antimicrobial effect of Butylated Hydroxy Anisole alone and in combination with EDTA against S. typhimurium and L. monocytogenes in culture media and Iranian White Cheese. At the first phase, Minimum Inhibitory Concentrations (MIC) of Butylated Hydroxy Anisole and EDTA against bacteria were measured. The samples of cheese made from pasteurized milk where infected experimentally with S. typhimurium and L. monocytogenes. They were divided as a control and other samples that have values of 2500, 5000 and 10000 μg/ml of BHA, and combination of 2500, 5000 and 10000 μg/ml of BHA and EDTA. In the next phase of study, bacteria were counted at different days. The results obtained from this study showed that using combination of BHA and EDTA can properly reduce number of S. typhimurium and L. monocytogenes in culture media and Iranian white cheese. This study demonstrated that BHA can be used for increase shelf life of Iranian white cheese Manuscript profile
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      76 - Study of microbial contamination in traditional ice cream (Case study: City of Urmia)
      Nahid Navidjoy Sima KarimzadehSadegh Anahita Dehghani Farshad BahramiAsl
      Abstract Regarding  that, the production of unpasteurized ice cream entitled to traditional ice cream is very common in our country and, on the other hand, the consumption of this product among different age groups especially children in  warm seasons is high More
      Abstract Regarding  that, the production of unpasteurized ice cream entitled to traditional ice cream is very common in our country and, on the other hand, the consumption of this product among different age groups especially children in  warm seasons is high, Thus, the pollution of this product to pathogenic microorganisms could be the reason for food poisoning and infections in consumer. In this research, samples of traditional ice cream were collected from four regions of Urmia City (10 samples from each region) and transferred with in compliance with the standard condition for microbial testing to laboratory. The examination was conducted based on standard laboratory methods and the results were analyzed. The results showed that, in the mesophilic bacteria count 80% of samples in the entrobacteria count 92.5% of samples were more than the standard rate. And in this case, the Escherichia coli bacteria 47.5%, Staphylococcus 45% and Salmonella 40% was isolated from others. According to the results, using healthy and pasteurized milk in making ice cream, creating good condition for transit, and keeping milk, training for producers of ice cream to observance of personal hygiene in all steps of manufacturing and distribution of ice cream is essential and recommended Manuscript profile
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      77 - Detection of virulence factors in Salmonella typhimurium and Salmonella enteritidis serotypes isolated from chicken meat in Chaharmahal va Bakhtiari province of Iran
      Hassan Momtaz Mohsen Ghaedamini Manochehr Momeni
      Salmonellosis, caused by bacteria from the genus Salmonella, is one of the most common foodborne illnesses and is manifested by diarrhea, mild fever, nausea, abdominal pains and even death. Its traditional diagnosis is based on culture, biochemical and serological techn More
      Salmonellosis, caused by bacteria from the genus Salmonella, is one of the most common foodborne illnesses and is manifested by diarrhea, mild fever, nausea, abdominal pains and even death. Its traditional diagnosis is based on culture, biochemical and serological techniques and for more accurate diagnosis several molecular methods have been developed. The epidemiology and prevalence of these bacteria in chicken meat samples is essentially unknown in Iran. in this study in order to isolation and characterization of Salmonella spp. 620 chicken meat samples were collected from supermarkets in Chaharmahal Va Bakhtiary province, Iran. from each samples genomic DNA was extracted and multiplex PCR method was developed. Results showed that 28 out of 620 samples (4.51%) were positive for Salmonella spp. and from these isolates 12 samples were S. typhimurium (1.93%), 10 samples were S. enteritidis (1.61%) and 6 samples were other species of Salmonella (0.96%). Results revealed that all of S. typhimurium and S. enteritidis isolates have virulence factors. Our results indicated that Salmonella spp. can contaminated chicken meat samples and it is essential to control the hygienic quality of chicken meat samples especially in slaughterhouse. Manuscript profile
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      78 - Microbial evaluation of herbal waters distributed in Kashan 2012
      Navid Mazroi Arani Reza Sharafati Chaleshtori
      Abstract In Iran, herbal waters are as traditional medicinal products and are widespreadly consumed. Therefore, microbial quality of these products in terms of public health is important. The aim of this study was evaluation of microbial quality of Herbal waters distrib More
      Abstract In Iran, herbal waters are as traditional medicinal products and are widespreadly consumed. Therefore, microbial quality of these products in terms of public health is important. The aim of this study was evaluation of microbial quality of Herbal waters distributed in Kashan 2012. In this descriptive study, the number of 132 herbal waters samples (27 traditional samples and 105 industrial samples) distributed in Kashan during 2012 year were purchased and transferred to laboratory. Then microbial tests such as total aerobic bacterial count, mold and yeast count, total coliforms, detection of enterococcus, Pseudomonas aeruginosa and sulphite reducing clostridia were evaluated based on national standard of Iran. The results showed that any one samples not contaminated to coliforms, E. coli and Sulphite reducing clostridia. 51.52% and 11.6% of samples were demonstrated total mesophilic bacteria and yeast counts more than the standard, respectively. Also, based on national standard of Iran, 57 industrial herbal waters samples (54.29%) and 4 traditional herbal waters samples were usable. Based on obtained results and contamination of some of these products to mold and yeast as well as high count of total aerobic mesophilic bacteria, pasteurization, good packaging material and good hygiene in processing procedure is recommended. Manuscript profile
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      79 - Study of Antifungal activity of Helichrysum arenarium essential oil on growth of Candida albicans and Saccharomyces cereviciae
      Hedyeh Davoudi Moghadam Ali Mohamadi Sani Masoumeh Mehraban Sangatash
      Abstract Helichrysum arenarium L. is a perennial herbaceous plant that belongs to asteraceae family and traditionally used to treat various diseases in many countries. In this study, the essential oil from the aerial parts of the H. arenarium L. was extracted with hydro More
      Abstract Helichrysum arenarium L. is a perennial herbaceous plant that belongs to asteraceae family and traditionally used to treat various diseases in many countries. In this study, the essential oil from the aerial parts of the H. arenarium L. was extracted with hydro-distillation using a clevenger approach and then its antimicrobial effects was studied on the growth of two yeasts species, Candida albicans and Saccharomyces cereviciae, using micro-dilution method at ten different concentrations. The results of this study indicated that the essential oil of H. arenarium had significant effects on reducing and eliminating tested yeasts (P< 0.05) but C. albicans with MIC=195.31 and MFC=3125 μg/ml indicated more resistant than S.cereviciae so that growth of S. cereviciae in all concentrations was lower than that of the C. albicans. The results revealed that the essential oil of H. arenarium had a significant anti yeast activity (P<0.05); therefore, it can be used as an antifungal agent in the food and pharmaceutical industries Manuscript profile
    • Open Access Article

      80 - Study of the effect of Satureja khuzestanica essential oil on the growth of Lactococcus garvieae in rainbow trout fillet at the refrigerator temperature
      Mahsa Ansari Mehdi Soltani Ebrahim Hosseini Abolghasem Kamali
      Abstract This research was done with the aim of study of the antimicrobial effects of Satureja khuzestanica on Lactococcus garvieae and on some spoilage characteristics of rainbow trout fillet in 4°C during 18 days of storage. To do so, 0.06, 0.12, 0.25 a More
      Abstract This research was done with the aim of study of the antimicrobial effects of Satureja khuzestanica on Lactococcus garvieae and on some spoilage characteristics of rainbow trout fillet in 4°C during 18 days of storage. To do so, 0.06, 0.12, 0.25 and 0.5 µg g-1concentration of the essential oil were added to the fillets. According to the results, the highest microbial load was observed in the control group and the lowest was observed in the fillets contained 0.5 µg g-1 of the essential oil (P≤0.05). The results also showed an increase in spoilage characteristics of the fillets at the end of the experiment showing a statistical difference for protein content (P≤0.05). The results of this study revealed that S.khuzestanica results in the reduction of L. garvieaein fish fillets as the concentration of 0.5 µg g-1 was the most effective. Manuscript profile
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      81 - Antibacterial characteristics of Pelargonium graveolens L. Hérit essential oil against food-borne pathogens
      Hoda Mohammadzade Ali Mohammadi Sani Shahram Shoeibi
      Abstract Using the potential of natural materials as special food additives for reducing the use of chemical preservatives in production of food stuff, considering their antimicrobial properties, is of great importance. In this research, the in vitro antibacterial effec More
      Abstract Using the potential of natural materials as special food additives for reducing the use of chemical preservatives in production of food stuff, considering their antimicrobial properties, is of great importance. In this research, the in vitro antibacterial effects of Pelargonium graveolens essential oil on Staphylococcus aureus, Escherichia Coli, Salmonella enterica and Listeria monocytogenes were studied. To do so, 4ml of the essence was prepared using Clevenger. Afterward, MIC and MBC were determined. MIC of S. aureus and E. coli was equal to 62.5 ppm and this figure for S. enterica and L. monocytogenes was 125 and 31.25 ppm, respectively. The results showed that, comparing to gram-negative bacteria, the gram-positive bacteria were more sensitive to this essential oil. The most sensitive tested microorganisms were L. monocytogenes, S. aureus, E. coli, and S. enterica. Finally, we found that the use of some ornamental plants for inhibiting bacteria can be a helpful approach in future researches. Manuscript profile
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      82 - A report on pasteurized orange juice contamination with Bacillus licheniformis
      Hossein Motamedi Amir TajBakhsh
      Abstract Presence of the Fruit juice spoilage bacteria, including Bacillus spp., can be problematic in fruit juice industry. The aim of this study was to isolate and identify the bacteria being able to tolerate orange juice pasteurization conditions. In this study, 16 s More
      Abstract Presence of the Fruit juice spoilage bacteria, including Bacillus spp., can be problematic in fruit juice industry. The aim of this study was to isolate and identify the bacteria being able to tolerate orange juice pasteurization conditions. In this study, 16 samples of orange juice were prepared and investigated in sterile conditions. 0.5 ml of each sample was inoculated in orange serum agar medium and incubated at 43 °C. The isolates were identified using biochemical and antibiogram tests. As a result, From 16 prepared samples, 2 samples were contaminated with bacteria. The bacterium isolated from these samples was able to grow at up to 50 °C on Orange Serum agar. This isolate was then identified as Bacillus licheniformis using the classification system of Linnaeus. Despite of having no pathogenic nature and producing the spores resistant to temperature and acid, Bacillus licheniformis is of great importance according to the viewpoint that it can be mistaken in primary detection by Bacillus of fruit juice which are the spoilage agents and differentiated tests should be done to separate them from Alicyclobacillus spp. for preventing from presenting wrong report. Furthermore, the isolation of these bacteria indicates the inappropriateness of processing conditions to eliminate bacteria from fruit juice that may provide possibilities for growth of fruit juice spoilage bacteria. Manuscript profile
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      83 - A report on pasteurized orange juice contamination to Bacillus licheniformis
      Hossein Motamedi Amir TajBakhsh
      Presence of the Fruit juice spoilage bacteria, including Bacillus spp., can be problematic in fruit juice industry. The aim of this study was to isolate and identify the bacteria being able to tolerate orange juice pasteurization conditions. In this study, 16 samples of More
      Presence of the Fruit juice spoilage bacteria, including Bacillus spp., can be problematic in fruit juice industry. The aim of this study was to isolate and identify the bacteria being able to tolerate orange juice pasteurization conditions. In this study, 16 samples of orange juice were prepared and investigated in sterile conditions. 0.5 ml of each sample was inoculated in orange serum agar medium and incubated at 43 °C. The isolates were identified using biochemical and antibiogram tests. As a result, From 16 prepared samples, 2 samples were contaminated with bacteria. The bacterium isolated from these samples was able to grow at up to 50 °C on Orange Serum agar. This isolate was then identified as Bacillus licheniformis using the classification system of Linnaeus. Despite of having no pathogenic nature and producing the spores resistant to temperature and acid, Bacillus licheniformis is of great importance according to the viewpoint that it can be mistaken in primary detection by Bacilluses of fruit juice which are the spoilage agents and differentiated tests should be done to separate them from Alicyclobacillus spp for preventing from presenting wrong report. Furthermore, the isolation of these bacteria indicates the inappropriateness of processing conditions to eliminate bacteria from fruit juice that may provide possibilities  for growth of fruit juice spoilage bacteria. Manuscript profile
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      84 - Prevalence of Staphylococcus aureusin meat and meat products
      Fatemeh Nonahal Ebrahim Rahimi Esmaeil AtaieSalehi
      Abstract Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made Staphylococcus has one of the most important factors causing the More
      Abstract Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made Staphylococcus has one of the most important factors causing the food poisoning. This study was done to investigate the prevalence of Staphylococcus in meat and meat products in Esfahan. From summer to winter 2012, 450 samples including raw beef (n=80), minced meat (n=80), lamb (n=80), goat (n=80), camel (n=50), hamburger (n=40), kebab (n=40) were randomly collected from butcheries and supermarkets in Isfahan, and analyzed for the presence of S. aureus. Totally, 234 (55/6 %) S. aureus were detected in meat and meat products. The mean count of S. aureus in positive samples was 8/3×102. The results indicated that although the percentage of contaminated samples was high, the number of bacteria presented in the samples is low with no potential risk for public health. To effectively manage the food production for decreasing diseases more epidemiologic investigations about S. aureustoxication are needed. Manuscript profile
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      85 - Evaluation of the effect of Cuminum ciminum and Satureja hortensis nano essences on isolated Aspergillus species from fish meal produced by factories in Mazandaran Province, Iran
      Mahsa Enayati Alireza Mokhtari Mansour Bayat Afshin Mohsenifar Reza Khansari
      Abstract The fungi producing mycotoxins reduce production of agricultural crops and cause irreparable loses to public health. Aspergillus is one of the most important fungi in the environment being capable to grow in nearly all compounds and organic materials and their More
      Abstract The fungi producing mycotoxins reduce production of agricultural crops and cause irreparable loses to public health. Aspergillus is one of the most important fungi in the environment being capable to grow in nearly all compounds and organic materials and their conidia are substantially scattered in the water, soil and air. Cuminum ciminum and Satureja hortensis are Iranian herbal medicines having many therapeutic effects. The aim of this study was to evaluate the inhibitory effect of Cuminum and Satureja nano essences on Aspergillus species isolated from fish meal produced by factories in Mazandaran Province. Twelve Aspergillus species isolated from 89 fish meal samples and inhibitory effect of Cuminum and Satureja nano essences were evaluated by micro dilution. The highest and least numbers were related to Aspergillus fumigatus and Aspergillus nidulance, respectively. The MIC and MFC foe different species was in the range of 1.56-6.25 and 3.12-25 μg ml-1. The results revealed the high effectiveness of the nano essence on Aspergillus species. Manuscript profile
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      86 - Comparison of PCR and culture for diagnosis Pseudomonas aeruginosa and Escherichia coli in packed Mineral water & drinking water
      Mahvash Pooyan Abbas Doosti Hamidreza Kabiri Fatemeh Drees
      Abstract The aim of the present study was to examine the presence of P. aeruginosa and E. coli in packed mineral and drinking water by using Polymerase Chain Reaction and microbial culture methods. In this study, 142 packed water samples were collected from active produ More
      Abstract The aim of the present study was to examine the presence of P. aeruginosa and E. coli in packed mineral and drinking water by using Polymerase Chain Reaction and microbial culture methods. In this study, 142 packed water samples were collected from active productive factories in various Provinces. Isolating and diagnosing the bacteria P. aeruginosa and E. coli were done by microbial culture and Polymerase Chain Reaction and the results were compared. E. coli contamination in PCR method, were evaluated as positive in 12 samples (14.6%) of mineral water, while all the samples in the microbial culture were negative (p=0.000). The results of study the contamination to E. coli in drinking water samples were negative based on PCR and microbial culture methods. Contamination of Pseudomonas aeruginosa was positive in 10 samples (12.1%) and 7 samples (8.5%) based on PCR and microbial culture methods, respectively (p=0.176). This value was 2 (3.3%) and 0 in drinking water by PCR and culture method, respectively (p=0.496). The results of this study indicates that Polymerase Chain Reaction can be high sensitive, specific, fast and easy method for identification. Manuscript profile
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      87 - Study on use of Nisinto to control Streptococcus iniae and Lactococcusgarvieae in rainbow trout fillet
      Laleh Roomiani Azadeh Mahmoodi Mansoureh Ghaeni Sara Ahmadi
      Abstract In this study, effect of different concentrations of nisin(0, 0.25, 0.5 and 0.75 µg/ ml) were conducted against zoonotic bacteria Streptococcus iniae and Lactococcusgarvieae in fish fillet of rainbow trout (Oncorhynchusmykiss) in refrigerator storage. Ly More
      Abstract In this study, effect of different concentrations of nisin(0, 0.25, 0.5 and 0.75 µg/ ml) were conducted against zoonotic bacteria Streptococcus iniae and Lactococcusgarvieae in fish fillet of rainbow trout (Oncorhynchusmykiss) in refrigerator storage. Lyophilized cultured of S. iniae (GQ850377) and L. garvieae (Ir-170A856bp) were obtained from Department of Aquatic Health and Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, and were used in this study. After determination of incubation dose of two bacteria (1.4×108 and 9.2×107 CFU/ ml) and preparation of nisin solution, incubation of bacterial was done in fish fillets. The results revealed that different concentrations of nisin were statistically different in reduction of bacteria comparing with control group (P<0.05). The highest inhibitory effect was observed in 0.75 µg/ ml 65.77% on L. garvieae and 60.88 % on S. iniae. Thatresults showed that nisin, as a preservative, can be effective in control of food-borne bacteria in foodstuff. Manuscript profile
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      88 - Antibacterial activity of liquid smoke and Pistacia atlantica Subsp. Kurdica on Lactobacillus bulgaricus
      Ghazal Rahimzadeh Asad Rokhzadi Samira Bahramian
      Abstract Smoked Doogh and Doogh flavored with Pistacia atlantica Subsp. Kurdica essential oil (mastic resin) are popular products in Kurdistan region. Anti-pathogenic effects of these essential oils have been shown in many studies. However, their effects on lactic acid More
      Abstract Smoked Doogh and Doogh flavored with Pistacia atlantica Subsp. Kurdica essential oil (mastic resin) are popular products in Kurdistan region. Anti-pathogenic effects of these essential oils have been shown in many studies. However, their effects on lactic acid bacteria in dairy products have never been investigated. Therefore, the aim of this study was to evaluate the effect of these essential oils on Lactobacillus bulgaricus. After isolation and purification of L. bulgaricus from Doogh, Mueller Hinton Agar was used for Disk Diffusion method to assess the susceptibility of the bacteria in different concentrations of the essential oils (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1, 2, 3, 4, 5 and 100%). The minimum inhibitory concentration for mastic resin and liquid smoke were 0.1% and 0.9 %, and the zone of inhibition was 11 and 6.5 mm, respectively. Furthermore, the inhibition zone diameters increased with the increasing in essential oil’s concentrations. According to the result of this study, L. bulgaricus is susceptible to mastic resin essential oil. Therefore, it will be recommended that using this essential oil should be restricted in dairy products. Furthermore, there is no problem in using it in fermented products containing L. bulgaricus in less than 0.9% concentrations of liquid smoke. At whole, the effect of essential oils on lactic acid bacteria should be investigated, before using them in dairy products. Manuscript profile
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      89 - Prevalence of Staphylococcus aureus in meat and meat products
      Fatemeh Nonahal Ebrahim Rahimi Esmail Ataie Salehi
      Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made  Staphylococcus has one of the most important factors causing the food More
      Staphylococcus aureus as the second or third important food-borne pathogen all around the world. Ability of development and production of heat-stable enterotoxin in a wide range of the food made  Staphylococcus has one of the most important factors causing the food poisoning. This study was done to investigates the prevalence of Staphylococcus in meat and  meat products  in  Esfahan. From summer to winter 2012, 450 samples including  raw beef (n=80), minced meat (n=80), lamb (n=80), goat (n=80), camel (n=50), hamburger (n=40), kebab (n=40) were randomly collected from butcheries and supermarkets  in Isfahan, and analyzed for the presence of S. aureus. Totally, 234 (55/6 %) S. aureus were detected in meat and meat products. The mean count of S. aureus in positive samples was 8/3×102. The results indicated that although the percentage of contaminated  samples was high, The number of bacteria presented in the samples is low with no potential risk for public health. To effectively manage the food production for decreasing diseases more epidemiologic investigations about S. aureus toxication are needed. Manuscript profile
    • Open Access Article

      90 - Study of Antifungal activity of Helichrysum arenarium essential oil on growth of Candida albicans and Saccharomyces cereviciae
      Hediyeh Davoudi Moghadam Ali Mohamadi Sani Masoomeh Mehraban Sangatash
      Helichrysum arenarium L. is a perennial herbaceous plant that belongs to asteraceae family and traditionally used to treat various diseases in many countries. In this study, the essential oil from the aerial parts of the H. arenarium L. was extracted with hydro-distilla More
      Helichrysum arenarium L. is a perennial herbaceous plant that belongs to asteraceae family and traditionally used to treat various diseases in many countries. In this study, the essential oil from the aerial parts of the H. arenarium L. was extracted with hydro-distillation using a clevenger approach and then its antimicrobial effects was studied on the growth of two yeasts species, Candida albicans and Saccharomyces cereviciae,using micro-dilution method at ten different concentrations.  The results of this study indicated that the essential oil of  H. arenarium had significant effects on reducing and eliminating tested yeasts (P< 0.05) but C. albicanswith MIC=195.31 and MFC=3125 µg/ml indicated more resistant than S. cereviciae so that growth of S. cereviciae in all concentrations was lower than that of the C. albicans. The results revealed that the essential oil of H. arenarium had a significant anti yeast activity (P<0.05); therefore, it can be used as an antifungal agent in the food and pharmaceutical industries. Manuscript profile
    • Open Access Article

      91 - Study of the changes in microbial load, putrescine and histamine in muscles of Otolithes ruber during storage in ice
      Ali Ghorbani Ranjbari Nazanin Ghorbani Ranjbari Ali Reza Anthology Manshadi Fatemeh Ghorbani Ranjbari
      Biogenic amines are small organic molecules formed by decarboxylase enzymes caused by bacteria in foodstuff. This study investigated the concentration of biogenic amines of putrescine and histamine in the fish stored in ice for an eighteen-day period in three day interv More
      Biogenic amines are small organic molecules formed by decarboxylase enzymes caused by bacteria in foodstuff. This study investigated the concentration of biogenic amines of putrescine and histamine in the fish stored in ice for an eighteen-day period in three day intervals using chromatography (days of 3, 6, 9, 15 and 18). In this study, the amine of histamine was not detected in the first and third day of storage; but utrescine was about 1.30±0.03 micrograms in the third day. The first day of detection of the both biogenic amines was the sixth day of storage. The primary concentration of histamine and putrescine was 1.51 and 1.30 micrograms per gram respectively, and was reach to 14.5 and 19 micrograms per gram respectively at the end of the period. Analysis of the results by ANOVA test indicated that there was a significant difference (P<0.05) in the concentrations of these amines between the first day (0) and the last day of storage. In addition, there was a significant relationship among the mesophilic bacterial load, increase of histamine, the psychrophile bacterial load and the biogenic amine of putrescine (P<0.05). Manuscript profile
    • Open Access Article

      92 - Table of Contents
      Manuscript profile
    • Open Access Article

      93 - Evaluation of antibacterial effects of Onions, methanol extracts and some antibiotics against the number of food born bacteria
      yones Anzabi Afshin javadi
      In this study, antibacterial effects of methanol extracts of red Onions with the scientific name; Allium cepa L.by the method of determining of MIC and MBC andthen perform antibiotic susceptibility test by the agar disk diffusion method onthe standard strains of some ba More
      In this study, antibacterial effects of methanol extracts of red Onions with the scientific name; Allium cepa L.by the method of determining of MIC and MBC andthen perform antibiotic susceptibility test by the agar disk diffusion method onthe standard strains of some bacteria in terms of food hygiene and were compared with the effects of some antibiotics.Results showed that the highest inhibitory effect was on Yersinia enterocolitica and Clostridium perfrigenes (MIC=62.5µg/mL) and the lowest same effect was on Staphylococcos aureus(MIC=500µg/mL).As well as the most bactericidal effect was on Yersinia enterocolitica (MBC=62.5µg/mL) and the lowest same effect was on Staphylococcos aureus (MBC=1000µg/mL).Also the results ofantibiogram tests indicated that the onions, methanol extracts have optimum antibacterial effect to the effects of the Vancomycin and Nalidixic acid as standard antibiotics and the greatest impact of this terms submitted on Clostridium perfrigenes(gram-positive bacteria) and Yersinia enterocolitica(gram-negative bacteria) thatstatistically; in the range of p Manuscript profile

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