Comparison of PCR and culture for diagnosis Pseudomonas aeruginosa and Escherichia coli in packed Mineral water & drinking water

Subject Areas : Aquatic Products

Mahvash Pooyan ¹ , Abbas Doosti ² , Hamidreza Kabiri ³ , Fatemeh Drees ⁴

1 - Institute of Standards and Industrial Research, Shahrekord, Iran.
2 - Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
3 - Biotechnology Research Center, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
4 - Shahrekord University of Medical Sciences, Shahrekord, Iran.

Received: 2014-09-15 Accepted : 2015-04-30 Published : 2014-12-01

Keywords: culture, PCR, Escherichia coli, Pseudomonas aeruginosa,

Abstract :

Abstract The aim of the present study was to examine the presence of P. aeruginosa and E. coli in packed mineral and drinking water by using Polymerase Chain Reaction and microbial culture methods. In this study, 142 packed water samples were collected from active productive factories in various Provinces. Isolating and diagnosing the bacteria P. aeruginosa and E. coli were done by microbial culture and Polymerase Chain Reaction and the results were compared. E. coli contamination in PCR method, were evaluated as positive in 12 samples (14.6%) of mineral water, while all the samples in the microbial culture were negative (p=0.000). The results of study the contamination to E. coli in drinking water samples were negative based on PCR and microbial culture methods. Contamination of Pseudomonas aeruginosa was positive in 10 samples (12.1%) and 7 samples (8.5%) based on PCR and microbial culture methods, respectively (p=0.176). This value was 2 (3.3%) and 0 in drinking water by PCR and culture method, respectively (p=0.496). The results of this study indicates that Polymerase Chain Reaction can be high sensitive, specific, fast and easy method for identification.