Isolation Alicyclobacillusacidoterrestris specific bacteriophage, the causative agent of fruit juice spoilage, from soil

Subject Areas : Aquatic Products

Amir TajBakhsh ¹ , S.Elham Rezatofighi ² , Hossein Motamedi ³ , Solmaz Rafiae ⁴

1 - MSc. Student, Department of Biology, Faculty of Science, ShahidChamran University, Ahvaz, Iran.
2 - Department of Biology, Faculty of Science, ShahidChamran University, Ahvaz, Iran.
3 - Department of Biology, Faculty of Science, ShahidChamran University, Ahvaz, Iran.
4 - MSc. Student, Department of Biology, Faculty of Science, ShahidChamran University, Ahvaz, Iran.

Received: 2015-01-20 Accepted : 2016-02-20 Published : 2015-02-01

Keywords: fruit juice spoilage, Alicyclobacillusacidoterrestris, Bacteriophage,

Abstract :

Abstract Alicyclobacillusacidoterrestris is the main bacterial agent of fruit juice spoilage and the methods for its elimination are much paid attention to. Recently, using this phage for controlling bacteria in food products has received special attention. The aim of this study was to isolate the specific bacteriophages of A. acidoterrestris from soil. In this study, the samples collected from the soil around the Cedruslibani (Cedar) trees were investigated after being centrifuged and filtered to seek the understudied phage. Bacterial culture equal to0.5 McFarland turbidity, isolated from fruit juice and confirmed by molecular and biochemical techniques, was prepared in AAB. Adding a certain amount of environmental samples allowed the phage to be replicated in the environmentcontainingbacteria. Then, the environment was centrifuged and the supernatant, which is the source of the phage, was filtered. The pure phage suspension was assessed in double agar method for studying the formation, shape and size of plaques. Plaque formation confirmed the presence of bacteriophages in the filtrate. Consequently, the heat and acid resistant bacteriophage was isolated from the soilaround the Cedruslibani (Cedar) trees. The results revealed the presence of specific bacteriophage of A. acidoterrestris. This phage can be used for eliminating of this bacterium in fruit juice. Stability of these bacteriophages in fruit juices its specific. Preserving the fruit quality side is of advantages of using this bacteriophage comparing to other methods.

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