A survey on bacterial contamination of fruit juice with Alicyclobacillus acidoterrestris using PCR and RFLP

Subject Areas : Aquatic Products

Hossein Motamedi ¹ , Amir TajBakhsh ²

1 - Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran.
2 - Graduated of MSc. of Microbiology, Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran

Received: 2014-07-01 Accepted : 2014-10-10 Published : 2014-12-01

Keywords: PCR, Alicyclobacillus, fruit juice spoilage, thermophilic bacterium,

Abstract :

Abstract Alicyclobacillus is an endospore forming and heat resistant bacterium that can cause changes in odor and color of fruit juice and make significant losses to juice industries. The purpose of this study was to identify this bacterium from different juices by PCR using specific primers. To do so, DNA extraction and PCR was done after culture and incubation. Of 24 tested samples, 5 cases were examined. As a consequence of enzymatic digestion with EcoR1 and electrophoresis, 118 and 528 bp fragments were obtained that are in accordance with RFLP pattern of Alicyclobacillus acidoterrestris. With regard to the obtained results, it can be claimed that contamination to this bacterium makes some problems in fruit juice industry. To diagnosis it better, PCR with high sensitivity can be used since it reduce the expenditures and save the time.