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Open Access Article
1 - Serum levels of malondialdehyde, glutathione peroxidase and superoxide dismutase in patients with non-Hodgkin's lymphoma in Zahedan
Hosnie Hoseini Parichehreh Yaghmaei Gholamreza Bahari Saeed Aminzadeh -
Open Access Article
2 - Diagnostic Value of Milk Total Antioxidant Status (TAS) and Malondialdehyde (MDA) in Cows with Subclinical Mastitis
, M. Shekari , F. Moosavinasab , O GhasemianThe sensitivity and specificity of milk somatic cell count (SCC) and bacterial culture as the golden standards for the diagnosis of bovine subclinical mastitis are low. Mastitis leads to the increased formation of free radicals, elevated oxidative and nitrosative stress MoreThe sensitivity and specificity of milk somatic cell count (SCC) and bacterial culture as the golden standards for the diagnosis of bovine subclinical mastitis are low. Mastitis leads to the increased formation of free radicals, elevated oxidative and nitrosative stresses, and decreased antioxidant activity in milk. Therefore, the aim of this study was to determine the diagnostic value of milk malondialdehyde (MDA) and total antioxidant status (TAS) for the diagnosis of subclinical mastitis in dairy cows. A total of 90 clinically healthy Holstein cows were randomly selected from dairy farms of Tehran province, Iran. Next, 55 of these cows were diagnosed to have subclinical mastitis based on an SCC of higher than 130×1000 cells/mL of obtained milk samples. Milk MDA level was determined by the thiobarbituric acid method and TAS was measured using a commercial kit (Randox Laboratories, England). The diagnostic sensitivity, specificity, and cut-off points for the tests were determined by the receiver operating characteristic analysis. Our findings revealed that the mean and median of milk MDA and TAS were significantly higher and lower in the milk samples collected from cows with subclinical mastitis, compared to the healthy subjects. Moreover, milk MDA level was found to have the highest clinical accuracy at the cut-off points of 44.5nmol/ml. The results showed that the sensitivity and specificity of this biomarker in milk for subclinical mastitis diagnosis was both 100%. According to the results of this study, MDA can be considered as potential reliable substitutes for SCC in the diagnosis of bovine subclinical mastitis. Manuscript profile -
Open Access Article
3 - Correlation of Total Antioxidants Levels and Malondialdehyde with Sperm Parameters and Chromatin integrity in Asthenoteratozoospermia Men
rahil jannatifar Zahra Ebrahimi Hamid piroozmanesh seyedeh Saeideh SahraeiBackground and purpose: Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. MoreBackground and purpose: Sperm dysfunction caused by reactive oxygen species (ROSs) is one of the major causes of infertility in men, which leads to, lipid peroxidation (LPO) and the formation of stable peroxidation products like Malondialdehyde (MDA) in seminal plasma. MDA is effective factor in reducing fertility. The aim of this study is to determine two biochemical markers of oxidative stress; TAC and MDA, and correlation to sperm parameters and chromatin integrity at asthenoteratozoospermia men. Materials and methods: In this study, a case-control study was carried out in two groups of 50 fertilized men and 50 asthenoteratozoospermia men in Qom IVF center; Iran. Semen analysis was performed according to WHO (2010) guidelines. DNA damage, protamine deficiency and TAC, MDA levels in all patients were measured by kit methods, respectively. Results: According to our results, the percentage of sperm parameters in the infertile men were lower than the fertile men (p <0.05). Also, there was a significant difference in DNA damage and protamine deficiency between the two groups (p <0.05). Lower TAC levels (1.82± 0.11 vs. 2.25 ± 0.13) and higher MDA levels (2.36 ± 0.09 vs. 1.97 ± 0.10) were observed in infertile men with asthenoteratozoospermia compared to fertile men.Conclusion: These results suggest that decreasing TAC and increasing MDA lead to low sperm parameters and high DNA integrity in sperm of asthenoteratozoospermia men. Therefore, evaluation of oxidative status and antioxidant defenses system may be as a useful tool for diagnosis and treatment of male infertility especially in idiopathic male infertility Manuscript profile -
Open Access Article
4 - Evaluation of Salinity Stress Tolerance in (Matthiola incana L.) under Salicylic acid Treatment
Samaneh Abdolmohammadi Jalal Omidi abdollah hatamzadeh Moazzam hassanpour asilSalinity is one of the environmental stresses that causes severe changes in the growth, physiology and metabolism of plants. It also causes various biochemical changes and physiological responses and affects virtually all plant processes. Considering the increasing tren MoreSalinity is one of the environmental stresses that causes severe changes in the growth, physiology and metabolism of plants. It also causes various biochemical changes and physiological responses and affects virtually all plant processes. Considering the increasing trend of salinity development and the lack of suitable lands for agriculture in the world, the use of saline resistant species or the use of compounds that reduce the effects of salinity stress and induction of resistance to stress in plants is very important. Salicylic acid, as a natural phenol, regulates growth and regulates the physiological and morphological processes of the plant. In order to evaluate the potential beneficial effects of salicylic acid on some physiological and morphological parameters of an experiment, a factorial experiment in a completely randomized design with 2 factors, salinity, including NaCl at 0, 100 and 150 mM and salicylic acid in 2 levels of 0 and 2 mM with 3 replications were conducted in the educational greenhouse of Faculty of Agriculture, Guilan University. At the end of the experiment, different parameters including plant height, dry weight and shoot weight, root dry weight and root, root length, malondialdehyde (MDA) concentration, protein content and relative leaf water content (LRWC) Made. In salinity stress, all growth parameters of plants, relative leaf water content, protein reduction and malondialdehyde increased. The application of salicylic acid increased all growth parameters, protein content, relative water content of the leaves, and eventually, salicylic acid, with its protective effect, reduced the peroxidation of lipids. Manuscript profile -
Open Access Article
5 - The response of oxidative stress indicators to grape seed extract supplementation and resistance training in bodybuilders
fariba joshaghani hamzeh rahmani amin yosefvandBlack grape seed extract (GSE) is one of the supplements that have strong antioxidant effects with flavonoid properties that can prevent or reduce cell damage. The purpose of this study was to investigate the response of oxidative stress indicators to grape seed extract MoreBlack grape seed extract (GSE) is one of the supplements that have strong antioxidant effects with flavonoid properties that can prevent or reduce cell damage. The purpose of this study was to investigate the response of oxidative stress indicators to grape seed extract supplementation and resistance training in bodybuilders. The methodology of the present research was conducted in the form of a semi-experimental design in two supplement and control groups with blood sampling on two occasions before and after taking the supplement in a double-blind manner. The statistical population of the present study consisted of 24 male bodybuilders with an age range of 15-19 years and they were divided into two groups: supplement and placebo. About 5 ml of blood was taken from the subjects in each of the fasting blood sampling sessions. Measurement of blood indices included antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), and lactate dehydrogenase (LDH). The normality of data distribution was done using ShapiroWilk tests. The findings of the research results indicate that performing bodybuilding resistance exercises caused a significant increase in cell damage indices (creatine kinase and lactate dehydrogenase) and malondialdehyde index and a significant decrease in total antioxidant capacity (P<0.05). Consumption of grape seed extract increased total antioxidant capacity. The present results show that performing bodybuilding activities increases the indicators of cell damage and oxidative stress, and the consumption of grape seed extract (100 mg per day) increases the total antioxidant capacity in male bodybuilders. Manuscript profile -
Open Access Article
6 - Ameliorative nature of flavonoid naringin: A comprehensive review of antitoxic effects
Zorawar Singh Shivam Sharma Adeshwinder Kaur -
Open Access Article
7 - The Effect of Ethanol Extraction of Aloe vera on Certain Biomarkers of Cardiovascular Disease in Mice Treated with Nanoparticles of Titanium Dioxide
Fereshteh Javan Masoomi Jila Talat Mehrabad Roghayeh Babri BonabInroduction & Objective: The effect of ethanol extraction of Aloe vera on certain biomarkers of cardiovascular disease in mice treated with titanium dioxide nanoparticles was studied.Material and Methods: Mice were received titanium dioxide nanoparticles with density of MoreInroduction & Objective: The effect of ethanol extraction of Aloe vera on certain biomarkers of cardiovascular disease in mice treated with titanium dioxide nanoparticles was studied.Material and Methods: Mice were received titanium dioxide nanoparticles with density of 100 milligrams per kg and ethanol extraction of aloe vera plant with density of 100, 200 and 300 milligrams per kg for 30 days. Plasma levels ofmalondialdehyde, rich sialic acid, troponin I and creatine kinase MB at the end of treatment was evaluated. The antioxidant activity of ethanol extraction in Aloe Vera using free radical DPPH and total phenol levels were considered.Results: The results showed that giving titanium dioxide nanoparticles through the stimulation of oxidative activity had a significant increase in plasma levels of malondialdehyde (MDA). Plasma levels of troponin I, creatine kinase MB and rich sialic acid also significantly increased. With increasing density of aloe vera, the total phenol and antioxidant activity of ethanol extraction increased. Giving the extraction significantly reduced the plasma levels of biomarkers of cardiovascular damage in mice treated with titanium dioxide nanoparticles.Conclusion:According to the results, ethanol extraction of aloe vera as an antioxidant compound is able to reduce the levels of cardiovascular damage in mice treated with titanium dioxide nanoparticles. Manuscript profile -
Open Access Article
8 - Effect Of Exhaustive Exercise and Supplement N-Acetyl Cysteine on TAC, MDA and CRP in Wistar Rats after a Single bout Of Exhaustive Exercise
Ali Aalizadeh Najmeh MobseriInroduction and ObjectivePhysical activity exhausting the release of reactive oxygen species (ROS) and nitrogen species activated (RNS) is a well has a dual role as both deleterious and beneficial species is known. N - Acetylcysteine (NAC) acetylating of the amino-L-cys MoreInroduction and ObjectivePhysical activity exhausting the release of reactive oxygen species (ROS) and nitrogen species activated (RNS) is a well has a dual role as both deleterious and beneficial species is known. N - Acetylcysteine (NAC) acetylating of the amino-L-cysteine, which is converted in the body into metabolites that are capable of stimulating glutathione synthesis may be resulting in the elimination of free radicals. Effective properties of complementary N – acetylcysteine reduces fatigue and effects on sub maximal contractions.Material and Methods: This study used 16 female Wistar rats weighing 150 to 200 g and (8 to 10 weeks old) were divided into 2 groups. First group effervescent tablets were given (600 mg) of N-acetyl cysteine dissolved in water) four hours before the experiment by gavages and group was taken as the control. Blood samples were taken 1 hour before starting the exhaustive exercise - immediately after the exhaustive exercise and after one hour at rest.Results: Significant decrease was observed in the concentration of MDA immediately after the exhaustive exercise in the group consumed NAC and blood concentration of TAC one hour after exhaustive exercise as compared with the control (P≤0.05). Reactive protein concentrations - C (CRP) in the period before, immediately and after exhaustive exercise did not show any significant difference.Conclusion:The results of our study demonstrated that orall administration of N-acetyl cystein at least four hours before an exhaustive exercise can significantly reduce the harmful effects of oxidative stress in rats. Manuscript profile -
Open Access Article
9 - Spark Motor Program reduced oxidative stress in boys with Down syndrome
Atefeh Jan Mohammadi Mehrzad Moghadasi -
Open Access Article
10 - Effects of Thymus Powder (Thymus Vulgaris L.) on Egg Quality and Some Ovarian Parameters in Laying Hens
Ahmad Minaei Javid Morteza Mehri Mohsen SharafiAn experiment was conducted in order to investigate the effect of different levels of Thymus vulgaris powder (0, 1, 2, and 3%) on performance, egg quality, some ovarian parameters, and level of malondialdehyde in the liver and follicular fluid of Hy-Line w36 laying hens MoreAn experiment was conducted in order to investigate the effect of different levels of Thymus vulgaris powder (0, 1, 2, and 3%) on performance, egg quality, some ovarian parameters, and level of malondialdehyde in the liver and follicular fluid of Hy-Line w36 laying hens. The hens were divided into 4 equal treatment groups and each treatment consisted of 4 replicates in a completely randomized design. In the period of 26 to 33 weeks of age, no significant difference was observed among the dietary treatments in terms of egg production, shell-less egg ratio, feed intake, and feed conversion ratio; but egg weight and egg mass were affected by 2% thymus powder (P<0.05). In the period of 34 to 39 weeks of age, shell-less eggs (%), egg production (%), and feed efficiency were not affected by thymus powder, but feed intake, egg mass, and egg weight were significantly affected by treatment 3 (P<0.05). The egg quality characteristics were measured at the end of weeks 26, 30, 34, and 38 of age. The results showed that only in week 26, the Haugh unit was significantly increased by 1 and 2% thymus powder (P<0.05). At the end of the experiment, the number of white, large yellow, and small yellow follicles, and level of malondialdehyde in the liver and follicular fluid were recorded and none of these were not affected by different levels of thymus powder. Overall, the experimental results showed that the use of 2% thyme powder in the laying hen diet improved the egg mass and egg weight, and also increased the Haugh unit during the peak production period. Manuscript profile -
Open Access Article
11 - Assessment Effect of Silicon on Physiological and Biochemical Traits of Corn (Zea mays L.) Under Salinity Stress Conditions
Afsaneh Bolbol Sharifloo Mojtaba Yousefi Rad -
Open Access Article
12 - Assessment Production of Natural Reactive Oxygen Species Affected on Dormancy Alleviation, Germination and Antioxidant System in Sunflower Seeds
Mahdi Shaaban Esfandiar Hassani Moghaddam Ezatollah Nabati Zahra Rahmati Motlagh -
Open Access Article
13 - Investigating of malondialdehyde levels in azoospermia model rats following a period of swimming exercises and stem cell transplantation
sahar kochaki Hajar Abbaszadeh parvin farzanegiThe purpose of this study was to investigate the malondialdehyde levels in azoospermia model rats following a period of swimming training and stem cell transplantation. Thirty 8-week-old rats were selected, and then the azoospermia model was created with the drug busulf MoreThe purpose of this study was to investigate the malondialdehyde levels in azoospermia model rats following a period of swimming training and stem cell transplantation. Thirty 8-week-old rats were selected, and then the azoospermia model was created with the drug busulfan with a dose of 40 mg. Rats were randomly divided into healthy control (n=5), sham control (n=5), azoospermia control(n=5), azoospermia+exercise (n=5), azoospermia+cell (n=5) and azoospermia+cell+exercise (n=5) groups. One month after the creation of the one-time model, stem cells were transplanted in the vas deferens in the amount of one million cells for each mouse, and after recovery, daily for 30 minutes in They swam 5 days a week for 8 weeks. Malondialdehyde levels were measured by thiobarbituric acid method and all calculations were done using SPSS/23 statistical software and at a significant level of p≤0.05. The results showed that induction of azoospermia model increased testicular tissue malondialdehyde levels (P=0.002) and exercise combined with cell therapy caused a non-significant decrease in testicular malondialdehyde levels in azoospermia model rats (P>0.05). In general, the results of the present research indicate an increase in malondialdehyde levels in testicular tissue cells of azoospermia model mice. On the other hand, regular aerobic exercise such as swimming along with cell therapy will probably help in controlling the effects of infertility diseases by reducing malondialdehyde levels and reducing oxidative stress. Manuscript profile -
Open Access Article
14 - Effect of selenium supplementation on antioxidant indices and metabolism-related hormones in rats exposed to heat stress
Hamid Ashrafi Ali Asghar Sadeghi Mohammad ChamaniAim: High temperature causes oxidative stress in the body of animals. Selenium is a trace mineral that affects the health and performance of the body in stressful conditions. There are limited studies on the effects of different doses of selenium in long-term heat stres MoreAim: High temperature causes oxidative stress in the body of animals. Selenium is a trace mineral that affects the health and performance of the body in stressful conditions. There are limited studies on the effects of different doses of selenium in long-term heat stress conditions on physiological parameters and health indicators. This study was conducted to determine the effects of different doses of selenium from selenium-methionine supplement on the antioxidant status (glutathione concentration, activity of superoxide dismutase and glutathione peroxidase), liver and kidney health index (the activity of transferase enzymes and creatinine concentration) and concentration of metabolism-related hormones (insulin, cortisol, triiodothyronine and tetra-iodothyronine).Materials and methods: In a completely randomized design, 25 female rats were randomly divided into five groups and five replicates. Five rats were placed at standard temperature during the experiment period and the rest of the rats were placed under heat stress (38 ± 2 °C for 6 hours/day). Rats in the negative control group (without heat stress) and positive control (heat stress) were fed with standard pellets without additive and the other three groups were fed with standard pellets plus 0.15, 0.30 and 0.45 mg selenium/kg as a selenium-methionine supplement for 30 days.Results: Rats receiving selenium had more hemoglobin and alanine transaminase enzyme activity (P=0.001) compared to the positive control group. Cortisol concentration in the positive control group was higher than the negative control group (P=0.001). Adding selenium to the diet of rats under heat stress decreased (P=0.001) the concentration of cortisol, and increased the concentration of insulin and triiodothyronine compared to the positive control group. The serum malondialdehyde of the positive control was higher than the negative control and other experimental groups (P=0.001).Conclusion: According to the results of this study, heat stress causes oxidative stress in the body and causes changes in the hormonal concentration and the activity of the body's enzymes. In overall, it can be concluded that higher selenium supplementation (0.45 mg/kg) is optimal for improving the liver and kidney indices, hormone concentration and antioxidant response in rats under heat stress conditions. Manuscript profile -
Open Access Article
15 - The effect of zinc-methionine supplementation on antioxidant status and expression of interleukin-4 and interleukin-6 genes in female rats under heat stress
Matin Jamei Ali Asghar Sadeghi Mohammad ChamaniIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc suppl MoreIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc supplements, which zinc combined with methionine, and have more intestinal absorption Aim: This study was done to evaluate the effects of different doses of zinc-methionine supplementation on the blood antioxidant status and the expression of interleukin 4 and 6 genes in rats exposed to heat stress.Materials and methods: In a completely random design, 20 female rats were divided into four treatment groups with five replicates. Rats were kept at a temperature of 31±2 °C for 20 hours and at a temperature of 38±2 °C for 4 hours per day (to create heat stress). The rats in the control group were fed standard pellets without additives, and the three experimental groups were fed standard pellets plus 15, 30 and 45 mg zinc-methionine supplement per kilogram of dry matter for 30 days. At the end of the experiment, a blood sample was collected from the inferior vena cava. Total antioxidant capacity, malondialdehyde concentration, antioxidant enzymes, liver enzymes in the serum and the expression of interleukin-4 and interleukin-6 genes were measured.Results: Serum zinc concentration increased linearly with increasing zinc dose in the diet (P<0.05). The serum activity of two enzymes, alanine aminotransferase and aspartate aminotransferase decreased linearly with increasing zinc-methionine dose in the diet (P<0.01). The highest activity of glutathione reductase and glutathione peroxidase was observed in the group receiving 30 mg/kg, and the lowest activity of these enzymes was observed in the control group. In general, the relative expression of the interleukin-4 gene was increased, and the relative expression of the interleukin-6 gene was decreased (P<0.05). The highest expression of interleukin-4 gene belonged to the group receiving 30 mg, and the highest relative expression of interleukin-6 gene belonged to the control group and the group receiving 15 mg/kg.Conclusion: The results of this study showed that the dose of up to 30 mg of zinc-methionine per kg of diet used in this study increases the activity of superoxide dismutase enzyme and glutathione peroxidase enzyme activity, decreases the activity of liver enzymes in the serum, increases the expression of the anti-inflammatory gene (Interleukin-4) and decreases the expression of inflammatory gene (Interleukin-6), which indicates the reduction of oxidative stress and the reduction of the effects of heat stress in the body of the rat. Manuscript profile -
Open Access Article
16 - Effect of Intensity and Volume of Exercise Training on Serum Levels of Ceruloplasmin and Malondialdehyde in Elite Handball Player Girls
zahra mehregan Najmeh RezaeianIntroduction: Imbalance between the production of free radicals and the antioxidant defense system leads to oxidative stress. The purpose of this study was to investigate the effect of four weeks of high intensity interval training (HIIT) versus four weeks of aerobic tr MoreIntroduction: Imbalance between the production of free radicals and the antioxidant defense system leads to oxidative stress. The purpose of this study was to investigate the effect of four weeks of high intensity interval training (HIIT) versus four weeks of aerobic training on serum levels of ceruloplasmin and malondialdehyde in elite handball player girls.Methods: Thirty young handball player girls (mean aged 16.85±1.4 years), with four years of membership in handball team of North Khorasan province, were selected and randomly divided into two groups of training and one control group. Subjects in experimental groups participated in four weeks of HIIT [running at intensity of 95-90 percentage of maximum heart rate and resting intervals of 1-3 minutes running at 60-50 percentage of maximum heart rate] and aerobic training (running at 50-70 percentage of maximum heart rate, 45-60 minutes per session), three sessions per week. The blood samples were taken factors before and 48 hours after last training session to assess blood factors. Data analysis was done by One-way ANOVA, paired t-test, and Pearson correlation, and P<0.05 considered significant.Results: Serum levels of MDA decreased significantly following aerobic training (P=0.044). Moreover, weight (P=0.009), body mass index (BMI) (P=0.002) and body fat percent (P=0.036) decreased significantly following HIIT. Furthermore, aerobic training resulted in significant decline in weight (P=0.008), BMI (P=0.015) and body fat percent (P=0.013).Conclusion: It seems that the volume of training influences on serum levels of MDA; however, ceruloplasmin levels was affected by neither intensity nor volume of training. Manuscript profile -
Open Access Article
17 - Wheat Response (Triticum aestivum L.) to Selenium under Normal Irrigation and Water Deficit Conditions
Mohammad Reza DadniaTo evaluate the effect of selenium (from sodium selenate) on increasing efficiency at water deficit condition a research was conducted in Karaj 1 wheat cultivar in 2014-2015 cropping year with split plot based on completely randomize block design with four replications MoreTo evaluate the effect of selenium (from sodium selenate) on increasing efficiency at water deficit condition a research was conducted in Karaj 1 wheat cultivar in 2014-2015 cropping year with split plot based on completely randomize block design with four replications in Karaj. Irrigation treatments with two levels, normal (I1) and interruption of irrigation at mid flowering (110 days after planting) (I2), which were assigned to main plots and selenium foliar application with six concentrations: 0 (S0), 5 (3.12 micro g.) (S1), 10 (6.24 micro g.) (S2), 15 (9.36 micro g.) (S3), 20 (12.5 micro g.) (S4) and 25 (15.62 micro g,) of sodium selenate per plant (S5) g.ha-1 to sub plots. The results showed that water deficit increased the rate of superoxide dismutase, catalase, glutathione peroxidase and malondialdehyde marker. Foliar application of selenium, increased contents of antioxidant (anti oxidation) enzymes under stress condition and was maximum in 20 g. selenium per ha-1. Content of malondialdehyde was decreased and caused significant increase in grain yield. Increasing antioxidants and reduction of malondialdehyde at normal condition was lower. On the other hand, negative effect of malondialdehyde on yield components, cellular membrane stability and antioxidant enzymes at humidity stress condition was higher than normal condition. Significant increase of cytoplasmic membrane stability, grain yield, grain weight and selenium content of seed was obtained when 15 and 20 g. selenium ha-1 were used as compared with those of higher and lower rates of selenium usage under water deficit and normal irrigation. Suppression of free oxygen radicals, and enzyme induction defense mechanisms against oxidative damage has been effective. Therefore, use of exogenous compounds such as selenium may increase the antioxidant capacity of plant against stress. Manuscript profile -
Open Access Article
18 - Variation of Forage Yields and some Agronomic and Physiological Characteristics of Kochia, Millet, Sorghum and Maize under Drought Stress
Hamid Najafinezhad Seyed Zabihollah Ravari Mohammad Ali JavaheriTo determine resistance of four species forage crops to drought stress an experiment was conducted for two years (2016-2017) in Kerman – Iran. In this study a randomized complete block design arranged in a split plot experiment with four replications was used. Dro MoreTo determine resistance of four species forage crops to drought stress an experiment was conducted for two years (2016-2017) in Kerman – Iran. In this study a randomized complete block design arranged in a split plot experiment with four replications was used. Drought stress treatments consisted of normal, moderate and severes drought levels (80, 130 and 180 mm cumulative pan evaporation respectively) were assigned to main plots and four forage plant types (maize, kochia, millet and sorghum) to subplots. Results indicated that with increasing of drought stress, leaf relative water content decreased but MDA (malondialdehyde) content increased. Among the four forage plants under study, kochia had the lowest MDA content. Chlorophyll a under moderate and severe drought stresses decreased by 16.3% and 20.7% respectively, as compared to normal drought. Kochia, in both years and under all levels of drought stresses, had the highest sodium percent in shoots. Sorghum had the highest amount of potassium content in shoot, as compared to other plants under severe and moderate levels of drought stresses. Sorghum produced the highest the forage yield at all levels of drought stresses and it produced highest fresh and dry (101241 kg.ha-1 and 30181 kg.ha-1) respectively under normal irrigation. Increasing drought stress resulted in the reduction of forage yield of all plant under study but yield reduction in kochia was lower than the yield of other plants. Millet and kochia with %11.9 and %9.82 crude protein contents respectively produced higher crude protein contents in this respect. Based on the results of this study, sorghum produced highest forage yield, millet good quality forage and kochia was the most tolerant plant to drought stress condition. Manuscript profile -
Open Access Article
19 - Response of Some Morpho-physiological Characteristics of Borago (Borago officinalis) to Nano Particles of Titanium Spraying
Roufiya Heydary Romy Payam Moaveny Hossein Hoseinpour Darvishy Mahdi ArefradBrago is an important medicinal plant. This study, thus, was conducted with different levels of nano particles of titanium (0.01, 0.03 and 0.05%) and elemental titanium on its different growth stages (4-5 leaf, flowering and after flowering) with four replications in a MoreBrago is an important medicinal plant. This study, thus, was conducted with different levels of nano particles of titanium (0.01, 0.03 and 0.05%) and elemental titanium on its different growth stages (4-5 leaf, flowering and after flowering) with four replications in a factorial experiment based on randomized complete block design at the Agricultural Field of Rement Village of Babol, Mazandaran, Iran, in 2012. Results showed that there were a significant differences between treatments of titanium, time of treatments and between their interactions. Among the traits under study, leaf fresh weight, leaf dry weight, stem fresh weigh at 1% and plant hight, stem dry weight, total plant fresh weight and total plant dry weight at %5 levels of probabilities showed significant differences for their interaction between different treatments of titanium and different stage of development. The highest plant height, leaf dry weight and plant fresh weight and plant dry weight were produced when nano particles of titanium applied after flowering stage. Where as, the highest fresh leaf, fresh stem weight and total plant fresh weights were obtained when plants treated with titanium at flowering stage. On the other hand, dry weight of flowering branches showed significant negative correlation with GPX antioxidant enzyme. The lowest GPX antioxidant enzyme was produced when nano particles of titanium were applied after flowering stage at 0.01% probability level. As a whole, the results of this experiment suggest that there are significant differences among titanium treatments when they are applied at different growth stages of this plant. This study also showed that application of nano particles of titanium after flowering of this plant increased its flowering branches. Manuscript profile -
Open Access Article
20 - The effects of active and inactivate Saccharomyces cerevisiae and their combination on performance, antioxidant levels and lipid peroxidation index in the blood of Japanese quail
حسین نیک پیران حامد منافی توحید وحدت پورConsidering that various effects of probiotics, prebiotics, and symbiotic on production and growth of broiler chicks has been reported previously, the aim of this study was to investigate the effects of probiotics and prebiotics and their mixture, on performance, antiox MoreConsidering that various effects of probiotics, prebiotics, and symbiotic on production and growth of broiler chicks has been reported previously, the aim of this study was to investigate the effects of probiotics and prebiotics and their mixture, on performance, antioxidant levels and lipid peroxidation index in the blood of Japanese quails. Thus, 192 day- old- Japanese quails were distributed randomly to 4 groups with 4 replicates of 12 male and female and evaluated for 42 days. The control group only received basal diet, but in the 2nd group (probiotic group) active saccharomyces cerevisiae 1 gr/Kg of feed, in the 3rd group (prebiotic group) inactivated saccharomyces cerevisiae 0.5 gr/Kg-feed of feed, and in the 4th group (synbiotic group) 0.5 gr/Kg saccharomyces cerevisiae and 250 mg/Kg-feed inactivated saccharomyces cerevisiae was added to the basal diet. Results indicated that mean serum levels of Malondialdehyde (MDA) was statistically different among males of different groups (p<0.05). Also the highest and lowest MDA levels were observed in the 4th and 3rd groups respectively with a statistically significant difference (p<0.05). Total antioxidant capacity (TAC) of plasma was not different statistically in the studied groups. On the other hand, mean feed consumption and body weight in the 2nd and 3rd groups was increased in comparison to control group and FCR was decreased significantly in comparison to control and synbiotic group. The best carcass efficiency was observed in the prebiotic group. The results showed that the employed probiotics and prebiotics were only effective in performance of Japanese quails, but did not have a specific effect on serum-MDA and plasma-TCA levels. Manuscript profile -
Open Access Article
21 - Protective and antioxidant effects of silymarin in sheep with fasciolosis treated by clorsulon
Mehrzad Aflatooni negar panahi Pejman Mortazavi Bahar Shemshadi Shapoor KakoolakiFasciolosis is a parasitic disease that causes economic losses. ‎In the present study, the protective effects of silymarin alone and in ‎combination with clorsulon were investigated in sheep naturally infected with ‎Fasciola hepatica. For this purpose, 40 Sa MoreFasciolosis is a parasitic disease that causes economic losses. ‎In the present study, the protective effects of silymarin alone and in ‎combination with clorsulon were investigated in sheep naturally infected with ‎Fasciola hepatica. For this purpose, 40 Sangsari breed sheep ‎were used, which were divided into five groups, including 1- healthy sheep without treatment 2- ‎fasciolosis affected sheep without treatment 3- fasciolosis affected sheep treated with silymarin 4- fasciolosis affected sheep treated with ‎Clorsulon, and 5- fasciolosis affected sheep treated with silymarin ‎and clorsulon. Biochemical parameters including triglyceride, total bilirubin, total protein, cholesterol, and albumin, were ‎measured. The serum level of malondialdehyde, total antioxidant capacity, and ‎hematological parameters were determined and compared among groups. Additionally, histopathological changes ‎were ‎‎‎investigated using light microscopy. Biochemical analysis showed significant ‎improvement in bilirubin levels, total protein, cholesterol, and albumin in sheep treated with silymarin ‎and clorsulon (p<0.05). Total antioxidant capacity increased in group treated with silymarin (p<0.05) compared to group 2, and the amount of oxidative stress decreased (p<0.01). Silymarin also improved hematologic factors and prevented a decrease in red blood cells (p<0.05).‎ Liver inflammation, ‎fibrosis, necrosis, and hyperplasia of the ‎bile ‎duct decreased significantly with the silymarin and ‎clorsulon group compared to the other groups ‎‎(p<0.0001). These findings ‎showed that using ‎silymarin in combination with clorsulon ‎improved their ‎therapeutic effects in treatment of ovine fasciolosis through enhancement of pharmacokinetic and pharmacodynamic relationship. Manuscript profile -
Open Access Article
22 - The Effect of Eight-Weeks of Aerobic Training with Nano-eugenol Supplementation on Pancreatic Tissue UPC3 Gene Expression and MDA Serum Levels in Diabetic Rats
Shirin Fotovat Khosro Jalali dehkordi Farzaneh TaghianAbstract Introduction: The use of exercise and herbal supplements to treat diseases and metabolic disorders has become popular among the people. Regarding the health-enhancing effects of aerobic training and eugenol, the present study aimed to investigate the effect of MoreAbstract Introduction: The use of exercise and herbal supplements to treat diseases and metabolic disorders has become popular among the people. Regarding the health-enhancing effects of aerobic training and eugenol, the present study aimed to investigate the effect of eight weeks of aerobic training with nano-eugenol supplementation on the gene expression of non-placental protein 3 (UPC3) in pancreatic tissue and serum malondialdehyde (MDA) levels in diabetic rats. Materials and Methods: In this experimental study, 20 diabetic rats were divided into four groups of five rats: 1) control, 2) aerobic training, 3) nano-eugenol, and 4) nano-eugenol + training. To investigate the effects of diabetes induction on research variables, five healthy rats were placed in a healthy control group. For eight weeks, groups 3 and 4 received 2 mmol of nano-eugenol by gavage daily, and groups 2 and 4 performed aerobic training at 30 m/s five days a week. Kolmogorov-Smirnov tests, one-way analysis of variance with Tukey post- hoc test were used to analyze the data (P≤0.05). Findings: Induction of diabetes had a significant effect on increasing UPC3 gene expression in pancreatic tissue and MDA serum levels (P=0.001). However, aerobic training with nano-eugenol had a significant effect on the reduction of UPC3 gene expression in pancreatic tissue (P=0.001) and serum levels (P=0.01). Conclusion: It seems that aerobic training along with the use of nano-eugenol has more favorable effects on improving UPC3 gene expression levels and MDA serum levels than the either one alone in diabetes. Keywords: Aerobic Training, Nano Eugenol, UPC3, Malondialdehyde, Diabetes Extended Abstract Introduction Type 2 diabetes mellitus is a common and growing global health issue. According to the American Diabetes Association, there are about 400 million people with diabetes in the world and it is expected to be increased to more than 600 million people in 2035. In recent years, exercise has been used as a non-pharmacological strategy to control diabetes and its complications. Exercise improves inflammatory diseases such as diabetes by reducing the secretion of inflammatory cytokines and increasing anti-inflammatory cytokines (3). Unpaired protein 3 (UPC3) catalyzes adaptive calorific value in muscle due to the increase of proton transfer from the inner mitochondrial membrane. Numerous studies have shown decreased expression of UPC3 mRNA and UPC3 protein in the skeletal muscle of subjects or as a result of aerobic training (4). Currently, the main effective treatment for diabetes is the use of insulin and synthetic drugs that lower blood glucose; but these compounds have several side effects. Given that medicinal plants have fewer side effects than synthetic drugs, therefore, researchers are looking for herbal compounds to treat or prevent this disease. Eugenolor 4-ally1-2-methoxyphenol is an aromatic molecule that is textured in the oils and in the essential oils of various plants, including cloves and cinnamon (6). Various studies have shown that natural antioxidants with phenolic structure play an important role in protecting body tissues against free radicals. According to the presented evidence, eugenol has an important protective effect against lipid peroxidation originated from free radical (7). Therefore, the present study was done to shed light on the ambiguities in this field due to the increasing prevalence of diabetes and its harmful effects on health and the incidence of its complications as well as the effect of antioxidant supplements and the specific type of physical activity on its control and lack of studies on the interactive effects of aerobic training and the use of nano-eugenolon the expression of UPC3 gene and MDA serum levels in diabetic patient. Materials and Methods In this experimental study, 25 male Wistar rats were purchased and transferred to the laboratory. Subsequently, 20 rats underwent intraperitoneal injection of streptozotocin (STZ). After confirming the rats as diabetic, fasting glucose-based diabetic rats were divided into four groups of five series including 1) training, 2) eugenol, 3) training + eugenol and 4) control. To investigate the effects of diabetes induction on research variables, the remaining five rats were placed in a healthy control group. For eight weeks, groups 3 and 4 received 2 mmol nano-eugenol daily by gavage (10) and groups 2 and 4 performed aerobic training at a speed of 30 m/min, five days a week according to Table 1 (11). It is noteworthy that eugenol was purchased specifically from Sigma and dissolved in dimethyl sulfoxide (DMSO). Nanoparticles were prepared according to the protocol with a concentration of 10% (10). Forty-eight hours after the last training session and after 14 hours of fasting, rats were injected intraperitoneally with a mixture of ketamine (10% at a dose of 50 mg/kg) and xylazine (2% at a dose of 10 mg/kg) were anesthetized and pancreatic tissue and serum of all rats were extracted. Then, UPC3 gene expression levels were measured by Real Time PCR method and MDA serum levels were measured by ELISA method using zellbio kits made in Germany. The sequence of primers of the present research is presented in Table 2. Kolmogorov- Smirnov tests, one-way analysis of variance with Tukey post- hoc test were used to analyze the data (P≤0.05). Findings The results of one-way analysis test showed that there was a significant difference in UPC3 (P=0.001) and MDA (P=0.001) levels of the studied groups. The results of Tukey post- hoc test showed that UPC3 in diabetic control (P=0.001), training (P=0.008), and eugenol (P=0.02) groups was significantly higher than healthy control group. UPC3 in the training + eugenol group was significantly lower than the diabetic control group (P=0.001) and training group (P=0.001). MDA serum levels in diabetic control groups (P=0.001), training group (P=0.02), and eugenol group (P=0.03) were significantly higher than healthy control group. In addition, UPC3 in training + eugenol group was significantly lower than the control group (P=0.02). Discussion The results of the present study showed that induction of diabetes had a significant effect on increasing UPC3 gene expression in pancreatic tissue and MDA serum levels. However, aerobic training had no significant effect on pancreatic UPC3 gene expression levels and MDA serum levels in diabetic rats. The findings of some studies show that UPC3 has significant changes due to the consumption of fatty foods, weight gain, as well as under the influence of training and weight loss. Fallahi et al., examined an eight-week period and an acute phase of high-intensity intermittent training (HIIT) on UCP2 and UCP3 in heart tissue of rats and reported an increase in UCP3 expression following the acute phase. They also reported that UCP2 and UCP3 gene expression decreased after a period of HIIT (14). To sum up what was mentioned above, it seems that response to UCPs expression is not related to the type of training; because HIIT was used in the study of Fallahi et al., but it is probably the intensity of the activity that plays a role in the expression of their genes. The researchers said that adaptation to exercise caused the reduction in the expression of UCPs, though; they showed an increase in UCP3 expression after an acute activity. Anyway, increasing or decreasing UCP3 gene expression has been reported inconsistently in various studies (14) and requires further discussion. The results of the present study also showed that eight weeks of eugenol use alone did not have a significant effect on pancreatic tissue UPC3 gene expression levels and MDA serum levels in diabetic rats. However, eight weeks of eugenol consumption along with training had a significant effect on reducing gene expression levels of UPC3 in pancreatic tissue and MDA serum levels in diabetic rats. Moreover, consumption of eugenol with training has a more favorable effect on reducing the expression levels of UPC3 gene in pancreatic tissue and MDA serum levels of diabetic rats than the training alone. In this regard, a significant decrease in the MDA index was observed in the research of Aziz Beigi et al., following incremental resistance training (21). Fetors et al., and Carabolt et al., in their studies also reported a significant decrease in MDA due to the training, the results of which are consistent with the present study (22, 23). Lack of control total calorie intake and calorie consumption of rats along with the measurement of metabolic- cellular parameters is one of the limitations of the present study; therefore, it is suggested that the effect of aerobic exercise with different intensities along with taking nano-eugenolsupplements with different doses on these variables be investigated in the future studies. Conclusion It seems that aerobic training accompanied by nano-eugenol consumption has more beneficial effects than each of them alone on the improving UPC3 gene expression levels and MDA serum levels in diabetics. Ethical Considerations Compliance with ethical guidelines This study was approved by the Ethics Committee of the Isfahan (Khorasgan) Branch of Islamic Azad University with ethical code: IR.IAU.KHUISF.REC.1398.178. Funding Isfahan (Khorasgan) Branch of Islamic Azad University Authors' contributions Design and conceptualization: Khosro Jalali Dehkordi and Farzaneh Taghian; Methodology and data analysis: Shirin Fotovvat; Supervision and final writing: Khosro Jalali Dehkordi and and Farzaneh Taghian. Conflicts of interest The authors declared no conflict of interest. Manuscript profile -
Open Access Article
23 - Investigation of some morphological and physiological responses of Moldavian balm (Dracocephalum moldavica L.) to selenium under salinity
Alireza Iranbakhsh Sara Saadatmand Ramazanali Khavarinejad Bita ZajiSalt stress is one of the most important environmental threats that adversely affects the growth, development, and productivity of plants; therefore, suitable approaches are needed to mitigate its negative effects on plants. For this purpose, a pot experiment was carrie MoreSalt stress is one of the most important environmental threats that adversely affects the growth, development, and productivity of plants; therefore, suitable approaches are needed to mitigate its negative effects on plants. For this purpose, a pot experiment was carried out in a completely randomized design with two treatments include salinity (0, 25, 50, and 75 mM) and foliar application of selenium (0, 5 and 10 µM Na2SeO4), and the possible role of selenium in regulating Moldavian balm salt tolerance was investigated. Foliar applications of selenium were applied after seedlings establishment at three stages, simultaneously with the application of salinity stress, and then at weekly intervals. Results indicated that growth indices, photosynthetic pigments, and relative water content of leaves in Moldavian balm plants were decreased significantly by various levels of salinity stress. Foliar application of selenium especially at the low concentration increased all the mentioned traits in stress conditions. In contrast, high level of selenium magnified the negative effect of salinity, so that the highest value of malondialdehyde, electrolyte leakage, and water potential were obtained by 10 µM selenium under 75 mM NaCl and caused the accumulation of proline and soluble sugars. This study indicates that selenium at low concentration plays a significant role in alleviating the harmful effects of salinity through photosynthetic pigments, maintaining membrane integrity, reducing water potential, accumulation of compatible solutes, and consequently, improved the performance in Moldavian balm plants growing under salt stress. Manuscript profile -
Open Access Article
24 - The effects of soil salinity in three regions on growth and biochemical parameters of Halocnemum strobilaceum (Case study: southeastern edge of Urmia Lake)
Nader Ahadi Latifeh pourakbarSoil salinity is on the increase around the world and it is considered as one of the most important challenges for the agricultural practices in world. Research has shown that saline soils limit the plant growth. Halocnemum strobilaceum plant belongs to the family of Ch MoreSoil salinity is on the increase around the world and it is considered as one of the most important challenges for the agricultural practices in world. Research has shown that saline soils limit the plant growth. Halocnemum strobilaceum plant belongs to the family of Chenopodiaceae and is a halophyte. The drying of Urmia Lake has led to the growth of this plant in saline soils of the region. This study was to investigate the effect of soil salinity on growth factors and osmolytes of H.strobilaceum plant in three regions (Chipgloo, Tapeh Chahar Borj, and Sahra Jen) southeastern edge of Urmia Lake. Soil and plant samples were analyzed for evaluation of growth parameters and physiological indices. The results indicated that Tapeh Chahar Bborj with silt-loamy texture had the minimum salinity (3.5 dS/m) and Sahra Jen region with sandy-loamy soil had the maximum salinity (17/43 dS/m). Also, results showed that the length, fresh and dry weight of shoots and roots, chlorophyll a, chlorophyll b, and carotenoids significantly decreased with increasing salinity while the Malondi aldehyde and proline and glycine betaine increased. Manuscript profile -
Open Access Article
25 - Determination of drought tolerance clones in Tea (Camellia sinensis L.) by investigating the activity of antioxidant enzymes
Mehdi Rahimi Mojtaba Kordrostami Mojtaba Mortezavi Sanam Safaei-ChaeikarIn order to investigate the activity of antioxidant enzymes of 14 tea clones under normal and drought stress conditions, two separate experiments were carried out in a randomized complete block design with two replications in 2017 at Fashalem Tea Research Station in Ras MoreIn order to investigate the activity of antioxidant enzymes of 14 tea clones under normal and drought stress conditions, two separate experiments were carried out in a randomized complete block design with two replications in 2017 at Fashalem Tea Research Station in Rasht. Irrigation in both designs was carried out routinely until July 22 followed by a drought stress treatment for one plot until August 22 when tea leaves were harvested. Tea leaves from each plot of the both experiments were then removed and transferred to a freezer at -80 °C. The activity of the antioxidant such as ascorbate peroxidase, superoxide dismutase, catalase, peroxidase, phenylalanine ammoniaase, lipid peroxidase, malondialdehyde, β-carotene, and lycopene were measured. The results showed that drought stress increased the activity of antioxidant enzymes. Based on the results obtained from the mean comparisons, clones 100, 399 and Bazri, had the highest activity for ascorbate peroxidase, superoxide dismutase, catalase, peroxidase, phenylalanine ammoniaase, and lipid peroxidase under drought stress conditions. On the other hand, these clones had the lowest contents of malondialdehyde. Clones 278 and 276 on the other hand, had the least values of antioxidant enzymes under drought stress conditions (except malondialdehyde) and were considered as sensitive clones. Manuscript profile -
Open Access Article
26 - Effect of salicylic acid application on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress
Mohammad Moghaddam Leila Mehdizade Sara FarsariThe aim of this research was to study the effect of salicylic acid (SA) usage on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress. For this purpose, a pot factorial experiment was performed base MoreThe aim of this research was to study the effect of salicylic acid (SA) usage on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress. For this purpose, a pot factorial experiment was performed based on a completely randomized design with 4 salinity levels (0, 50, 100 and 150 mM NaCl), 4 SA levels (0, 150, 300 and 450 mg/L) and three replications. The measured traits included total soluble protein, malondialdehyde (MDA), hydrogen peroxide, glycine betaine, some antioxidant enzyme activities, and essential oil production. The interaction effect of the treatments on all of the studied traits were significant at 1% P value. The results showed that the highest amount of total soluble protein, MDA, hydrogen peroxide, glycine betaine, ascorbate peroxidase and superoxide dismutase activities were observed at 150 mM salinity level and application of SA at 300 mg/L causes to decrease total soluble protein and glycine betaine, and 150 mg/L of SA causes to decrease MDA. But application of this plant growth regulator had no significant effect on hydrogen peroxide. Also, the results showed that catalase enzyme activity and essential oil content at the highest salinity level (150 mM) reached to the lowest amounts. In total, the results of this study indicated that the SA application especially at 300 mg/L causes to improve the studied traits in this experiment and its usage under stress conditions is recommended. Manuscript profile -
Open Access Article
27 - The effect of biosynthesized silver nanoparticles on some physiological and biochemical parameters of viola tricolor (Viola tricolor L.)
arefeh hassanvand Sara Saadatmand hossin lariyazdi Alireza iranbakhshEmploying biosynthesized nanoparticles plays an important role in increasing efficiency of agricultural practices. In recent years, the use of nanoparticles in plants has been considered as pesticides, protective agents, and nutrients. Viola tricolor belongs to the Viol MoreEmploying biosynthesized nanoparticles plays an important role in increasing efficiency of agricultural practices. In recent years, the use of nanoparticles in plants has been considered as pesticides, protective agents, and nutrients. Viola tricolor belongs to the Violaceae family, which has antibacterial, anticancer, and antiviral properties. In this study, silver nanoparticles were synthesized using silver nitrate and Viola tricolor extract to investigate the effect of different concentrations of silver nanoparticles on the physiologic and biochemical indexes of Viola tricolor. Results showed that different growth parameters including root and stem fresh weight, root and shoot length, and protein content significantly increased under AgNPs. The highest levels of these indices were observed at 0, 10, 50 and 100 ppm silver nanoparticles, respectively. Proline and carbohydrates also increased under different concentrations of AgNPs compared with the control and the highest values of these indices were observed under 100 ppm silver nanoparticles. The contents of secondary metabolites, including phenol and flavonoids, were affected under 100 ppm AgNP showing the highest increase. The maximum increase in the anthocyanin content was observed at 10 ppm AgNPs. Analysis of the antioxidant enzyme activities showed that they increased under all AgNPs concentrations of the study. Increases in the activities of antioxidant enzymes (catalase and glutathione reductase) under AgNPs treatments led to a decrease in MDA content. Based on the results of the current study, silver nanoparticles are suggested as proper stimulants for increased growth and production of antioxidant properties. Manuscript profile -
Open Access Article
28 - The effect of root and foliar application of selenium on some physiological and biochemical responses of wheat (Triticum aestivum L.) under salt stress
Masoumeh Abedini Meysam Garebaghi Sakineh MoradkhaniIn order to investigate the effects of foliar and root application of selenium (0 and 20 µM) on some physiological and biochemical parameters of wheat (Triticum aestivum L.) var. Chamran under salt stress (0 and 100 mM NaCl), an experiment was conducted hydroponic MoreIn order to investigate the effects of foliar and root application of selenium (0 and 20 µM) on some physiological and biochemical parameters of wheat (Triticum aestivum L.) var. Chamran under salt stress (0 and 100 mM NaCl), an experiment was conducted hydroponically based on a completely randomized factorial design with three replications at Payame Noor university in 2017. Results showed the significant decreases in growth parameters, chlorophyll to carotenoids ratio, and chlorophylls and total protein contents while significant increases were recorded in malondialdehyde, proline, anthocyanin, and total phenol contents of plant shoots under salt stress. Under control condition, both forms of selenium application significantly decreased the root length while increasing the total phenol content. Moreover, significant increases in proline and carotenoids contents were found under foliar application of selenium in control plants. Under salinity stress, foliar application of selenium significantly increased the root length and shoot fresh weight and its application at both forms improved the other growth parameters of plants. Likewise, significant increases in protein and chlorophylls contents of plants by foliar application and soluble sugars and total phenols contents of plants by both forms of application of selenium were attained under salinity. Only foliar form of application of selenium could decrease the malondialdehyde content of plants under salinity stress. According to the findings, selenium application, especially through leaves, played a remarkable role in ameliorating the effects of salt stress in wheat. Manuscript profile -
Open Access Article
29 - Evaluation of biochemical parameters of Portulaca oleracea under chromium and salinity
Zahra Talebzadeh Raheleh Rahbarian mohebat Nadaf Hamid SobhanianThe aim of this study was to investigate the interaction of salinity and chromium stress on some biochemical characteristics of portulaca oleracea. This study was conducted in a completely randomized greenhouse design with 3 replications. 4 salinity levels (0, 4, 8 and MoreThe aim of this study was to investigate the interaction of salinity and chromium stress on some biochemical characteristics of portulaca oleracea. This study was conducted in a completely randomized greenhouse design with 3 replications. 4 salinity levels (0, 4, 8 and 12 ds/m) were provided using sodium chloride every 4 days through irrigation and chromium treatment levels (0, 7, 14, 21 and 28 mg/kg dry weight of soil) and before from seed cultivation in soil, different levels of chromium treatment were obtained by increasing potassium dichromate to soil. The levels of Soluble and insoluble carbohydrates in alcohol, proteins, proline, malondialdehyde and hydrogen peroxide were measured after 60 days of plant cultivation. The results showed that with increasing levels of chromium and salinity, the amounts of proline, peroxide, malondialdehyde, soluble carbohydrates increased, but insoluble carbohydrates and proteins decreased. The lowest amount of insoluble carbohydrates and proteins and the highest amount of proline, peroxide, malondaldehyde and alcohol-soluble carbohydrates were observed in the combined stress of salinity levels of 12 ds/m and chromium 28 mg/kg. The combined stress of salinity and chromium caused a further reduction in insoluble carbohydrates and portulaca protein. Manuscript profile -
Open Access Article
30 - Evaluation of some root growth traits and activity of enzyme and nonenzyme antioxidants of different cultivars of durum wheat (Triticum turgidum var. durum) effected by phosphorous fertilizer and mycorrhizal fungi in rainfed condition
Houshang Naseri Rad Rahim Naseri In order to evaluate the effect of mycorrhizal fungi and phosphorous fertilizer on some root growth traits and activity of enzyme and nonenzyme antioxidants of durum wheat in rainfed conditions, an experiment was carried out as factorial based on a randomized comp More In order to evaluate the effect of mycorrhizal fungi and phosphorous fertilizer on some root growth traits and activity of enzyme and nonenzyme antioxidants of durum wheat in rainfed conditions, an experiment was carried out as factorial based on a randomized complete block design with three replications at Sarableh Agricultural Research station during growing season 2018-2019. Four cultivars of durum wheat (Dehdasht, Zahab, Savarz and Saji) as the first factor and five levels of fertilizer source (control, 25 and 50 kg.ha-1 P, mycorrhizal fungi (GM), mycorrhizal fungi + 25 kg.ha-1 P) as the second factor were considered. The average comparison results of simple effects indicated that Zahab and Saji cultivars among cultivars and combination treatment of mycorrhizal + 25 kg / ha P and then 50 kg / ha P had the greatest effect on improving the studied traits. The interaction effect of cultivars and fertilizer sources revealed that the combined use of phosphorous fertilizer and mycorrhizal fungi had better results compared to their use alone. So that, the highest fresh and dry weight, length density, and root water content and activity of ascorbate peroxidase, peroxidase, catalase, superoxide dismutase and glutathione synthetase was obtained in combined treatment of mycorrhizal and phosphorus fertilizer at Zahab and Saji cultivars. However, the lowest specific root length, and malondialdehyde and hydrogen peroxide activity were obtained in the same treatment and cultivars. In general, the results showed that inoculation with mycorrhizal fungi in rainfed conditions, especially in Zahab and Saji cultivars, in addition to reducing the application of phosphorus fertilizer can improve root growth characteristics and activity of enzyme and nonenzyme antioxidants. As a result, it reduces the peroxidation of membrane lipids (the decline in production of malondialdehyde and hydrogen peroxide) and increases drought stress tolerance. Manuscript profile -
Open Access Article
31 - The effect of growth-promoting bacteria on yield and some biochemical characteristics of black cumin (Nigella sativa L.) under drought stress conditions
Maedeh Yousefian Shahaboddin Mirinejad Zahra RahamiAn approach to overcome drought stress is using growth-promoting bacteria. This study was carried out to investigate the effect of Azospirillum growth-promoting bacteria under drought stress on yield and biochemical characteristics of Nigella sativa L. in the crop year MoreAn approach to overcome drought stress is using growth-promoting bacteria. This study was carried out to investigate the effect of Azospirillum growth-promoting bacteria under drought stress on yield and biochemical characteristics of Nigella sativa L. in the crop year 2019-2020 in Servak village of Yasouj province in Kohgiluyeh and Boyer-Ahmad province. The experiment was performed as a factorial split plot in a randomized complete block design with three replications. The main factor was irrigation in three levels (irrigation after using 30% soil moisture (control), irrigation after using 60% soil moisture, and irrigation after using 90% soil moisture) and the sub-factor of Azospirillum growth-promoting bacteria in two levels (no inoculation and inoculation of seeds with bacteria). Results showed that inoculation of growth-promoting bacteria increased yield and carbohydrate under moderate stress conditions. In high stress conditions, carotenoid and malondialdehyde had significant changes. Chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids had significant changes under both moderate and severe stress. On the other hand, chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids had significant changes in both moderate and high stress. The highest concentrations of total chlorophyll (1.01 mg / g fresh weight) and carotenoids (0.77 mg / g fresh weight) were observed in irrigation after consuming 30% soil moisture and inoculation with bacteria. The highest yield (1072 kg / ha) was observed under consuming 30% soil moisture and bacteria. Measuring the rate of plant growth is suggested at different stages to determine the effect of stress in each period to get a perfect interpretation of the effect of stress on the plant. Manuscript profile -
Open Access Article
32 - Determining the relationship between physical activity levels and some psychological factors with lipid peroxidation and antioxidant index in middle-aged women
tahereh abbasiAbstractThe aim of this study was to determine the relationship between the level of physical activity and some psychological factors on lipid peroxidation and antioxidant index in middle-aged women. The research method of the present study is correlational. The statist MoreAbstractThe aim of this study was to determine the relationship between the level of physical activity and some psychological factors on lipid peroxidation and antioxidant index in middle-aged women. The research method of the present study is correlational. The statistical sample of this study included 30 middle-aged female employees of Gorgan Islamic Azad University who were systematically selected randomly. A researcher-made questionnaire to determine the level of physical activity and the standard Lavibund (1995) stress, anxiety and depression questionnaire were used to collect research data. Pearson correlation test was used to analyze the data. The findings of the present study indicated that there is a significant relationship between the level of physical activity with lipid peroxidation and the total antioxidant index of middle-aged women. There is also a significant relationship between some psychological factors (stress, anxiety and depression) with lipid peroxidation in middle-aged women. While it was observed that there is no significant relationship with the total antioxidant index of middle-aged women. Findings indicate that the level of oxidative enzymes has a significant relationship with physical activity and psychological factors (stress, anxiety and depression), so it is important to pay attention to physical activity and modulate the level of psychological factors for health. Manuscript profile -
Open Access Article
33 - اثر الاژیک اسید بر استرس اکسیداتیو ناشی از ایسکمی/هیپوپرفیوژن مغزی در موش صحرایی نر
مریم رفیعی راد خدیجه قاسم زاده دهکردیمقدمه و هدف: رادیکال های آزاد در طی فرآیندهای ایسکمی تولید می شوند. رادیکال های آزاد در بیماری های عصبی نقش دارند و آنتی اکسیدان ها نقش محافظتی دارند. الاژیک اسید یک ترکیب پلی فنلی با خواص آنتی اکسیدانی است که در میوه هایی مانند انار، زغال اخته و انواع توت یافت می شود. Moreمقدمه و هدف: رادیکال های آزاد در طی فرآیندهای ایسکمی تولید می شوند. رادیکال های آزاد در بیماری های عصبی نقش دارند و آنتی اکسیدان ها نقش محافظتی دارند. الاژیک اسید یک ترکیب پلی فنلی با خواص آنتی اکسیدانی است که در میوه هایی مانند انار، زغال اخته و انواع توت یافت می شود. این مطالعه با هدف بررسی اثر 14 روز تجویز خوراکی الاژیک اسید 50 میلی گرم بر کیلوگرم بر شاخص های استرس اکسیداتیو مغز پس از انسداد دائمی دو طرفه شریان کاروتید مشترک و یا ایسکمی/ هیپوپرفوزیون در موشهای صحرایی نر بالغ انجامگرفت. مواد و روشها: تعداد 30 سر موش به گروه های آزمون و کنترل تقسیم شدند. برای ایجاد ایسکمی در موش صحرایی، شریان های کاروتید عمومی بوسیله ابزارهای بخیه ی پوستی با دو گره محکم در حول رگ (بالا و پایین) مسدود و سپس شریان ها به طور کامل از وسط قطع گردیدند. سپس مغز موشها جهت ارزیابی میزان پراکسیداسیون لیپیدی و اندازه گیری میزان گروه های تیول(-SH) استخراج شدند. نتایج و بحث: نتایج تحقیق حاضر نشان داد که میزان مالون دی آلدئید و تیول در گروه ایسکمی نسبت به گروه کنترل افزایش معنی داری داشته است و میزان مالون دی آلدئید و تیول در گروه ایسکمی دریافت کننده الاژیک اسید نسبت به گروه ایسکمی کاهش معنی داری داشت. توصیه کاربردی/صنعتی: الاژیک اسید با خاصیت آنتی اکسیدانی قوی توانستهاست اثرات ایسکمی ازجمله تولید رادیکالهای آزاد را بهبود ببخشد. Manuscript profile -
Open Access Article
34 - اثرات آنتی بیوتیکی و آنتی اکسیدانی عصاره متانولی برگ گیاه Momordica charantia بر فشار خون ناشی از آلوکسان در موش صحرایی
ساندی افوگ الوفکه فلایی بلسین اگونپلو آدمولا اویابمی تمیدایو اوموبوال مومو یکوبو اولووافمی اوگونتیبجو آدلو آدداپوBackground & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momo MoreBackground & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats. Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated. Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group. Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus. Manuscript profile -
Open Access Article
35 - مکانیسم سیستم دفاع آنتی اکسیدانی غیر آنزیمی در اندام های مختلف Catharanthus roseus برای محافظت از غشای سلولی
شاهین مردانی نژاد رمضانعلی خاوری نژاد سارا سعادتمند فرزانه نجفی پرویز آبرومند آذرمقدمه و هدف: وقتی سطح رادیکال های آزاد افزایش می یابد و سیستم های آنزیمی و ملکول های کوچک آنتی اکسیدانی قادر به محافظت از موجود زنده نباشد، دریافت آنتی اکسیدان از منابع خارجی لازم به نظر می رسد. این مطالعه با هدف بررسی توان آنتی اکسیدانی قسمت های مختلف کاتارانتوس Moreمقدمه و هدف: وقتی سطح رادیکال های آزاد افزایش می یابد و سیستم های آنزیمی و ملکول های کوچک آنتی اکسیدانی قادر به محافظت از موجود زنده نباشد، دریافت آنتی اکسیدان از منابع خارجی لازم به نظر می رسد. این مطالعه با هدف بررسی توان آنتی اکسیدانی قسمت های مختلف کاتارانتوس روزئوس انجام شد. مواد و روش ها: پتانسیل آنتی اکسیدانی عصاره های اتانولی ریشه ها، ساقه ها، برگ ها، گل ها، غلاف دانه ها و دانه های گیاه کاتارانتوس روزئوس بر اساس مهار رادیکال آزاد 1، 1-دی فنیل-2-پیکریل هیدرازیل (DPPH)، مهار پراکسیداسیون لیپیدی بوسیله روش فریک تیوسیانات (FTC) و مهار مالون دی آلدئید با روش تیوباربیوتریک اسید (TBA) در مقابل استانداردهای بوتیلیت هیدروکسی تولوئن (BHT)، بوتیلیت هیدروکسی آنیسول (BHA) و آسکوربیک اسید مورد بررسی قرار گرفت. نتایج و بحث: محتوای فنلی در ریشه (58/2±61/61 میکروگرم معادل گالیک اسید در هر میلی گرم وزن خشک عصاره) طبق روش فولین-سیوکالتیو بیشتر از سایر اندامها بود. در مهار نیمی از رادیکال های آزاد DPPH عصاره ریشه، بذر و برگ به ترتیب با 02/2±9/238 ، 42/1±78/253 و 56/2±95/277 میکرو گرم در میلی لیتر بهترین عملکرد را داشتند. در مهار پراکسیداسیون لینولئیک اسید، عصاره ریشه بهترین قدرت مهاری را بعد از آسکوربیک اسید و BHT داشت و عصاره های برگ و دانه عملکردی مشابه با BHA داشتند. در مهار مالون دی آلدئید عصاره ریشه و دانه به ترتیب با 54/0±68/78 و 66/0±44/77 درصد نسیت به سایر عصاره ها بهترین عملکرد را داشتند. ضریب تشخیص پیرسون بین محتوای فنلی و ظرفیت آنتی اکسیدانی عصاره ها مطابق روش های DPPH، FTC و TBAبالا و معادل 838/0-، 895/0 و 740/0 بود. توصیه کاربردی/ صنعتی: نتایج این تحقیق می تواند نوید بخش استفاده از توان آنتی اکسیدانی اندام این گیاه بویژه در مهار رادیکال های آزاد و پراکسیداسیون لیپیدی باشد. Manuscript profile -
Open Access Article
36 - Total Antioxidant Capacity and Malondialdehyde Level in Plasma of Broiler Chicks Fed Diet Containing Different Levels of Ginger (Zingiber officinale)
A.A. Sadeghi W. Izadi P. Shawrang M. Chamani M. Aminafshar -
Open Access Article
37 - اثر عصاره آبی الکلی کاسبرگ و برگ چای ترش بر عملکرد، کیفیت تخم مرغ، سامانه ایمنی و تعادل اکسیدانی مرغ های تخمگذار
ش. ثابت سروستانی س.م. حسینی س.ه. فرهنگ فراین آزمایش با هدف بررسی اثر عصاره آبی-الکلی گیاه چای ترش، بر عملکرد، کیفیت تخم­ مرغ، سامانه ایمنی و تعادل اکسیدانی مرغ­ های تخمگذار در طول شرایط محیطی خنثی طراحی شد. 200 قطعه مرغ تخم­گذار سویه های-لاین W-36 (در سن 23 هفتگی) به صورت پنج تیمار آزمایشی با چهار Moreاین آزمایش با هدف بررسی اثر عصاره آبی-الکلی گیاه چای ترش، بر عملکرد، کیفیت تخم­ مرغ، سامانه ایمنی و تعادل اکسیدانی مرغ­ های تخمگذار در طول شرایط محیطی خنثی طراحی شد. 200 قطعه مرغ تخم­گذار سویه های-لاین W-36 (در سن 23 هفتگی) به صورت پنج تیمار آزمایشی با چهار تکرار و 10 پرنده در هر تکرار تقسیم شدند. پرندگان تیمار یک با جیره بر پایه ذرت-سویا بدون عصاره تغذیه شدند، در حالیکه، تیمارهای دو و سه به ترتیب، 300 و 700 میلی­گرم در کیلوگرم عصاره برگ (HSLE) و تیمارهای چهار و پنج به ترتیب، 300 و 700 میلی­گرم در کیلوگرم عصاره کاسبرگ­ (HSLE) چای ترش را دریافت کردند. داده­ های عملکرد شامل تولید تخم ­مرغ، مصرف خوراک، توده تخم­ مرغ تولیدی و ضریب تبدیل خوراک به طور هفتگی ثبت شدند. شاخص­ های کیفیت تخم ­مرغ در هر 28 روز از دوره آزمایش مورد بررسی قرار گرفتند. از هر تکرار دو پرنده انتخاب و برای تعیین سامانه ایمنی و MDA پلاسما خون­گیری شد. همچنین از دو زرده تخم ­مرغ در هر تکرار برای بررسی MDA، کلسترول و تریگلیسرید زرده استفاده شد. مقایسه نتایج نشان داد، HSCE در سطح 700 میلی­گرم بر کیلوگرم نسبت به گروه شاهد میزان تخمگذاری را در زمان اوج تولید (P<0.05) تا 8 درصد افزایش داد. با این حال، تعادل اکسیدانی و عملکرد ایمنی بین گروه­های آزمایشی متفاوت نبود. مقاومت پوسته، ضخامت پوسته، وزن پوسته و شاخص شکل تخم ­مرغ به وسیله تیمارها تحت تأثیر قرار گرفتند (P<0.05). مرغ­های دریافت­کننده 700 میلی­گرم بر کیلوگرم HSLE نسبت به گروه شاهد (44.74 در برابر 47.67) به ­طور معنی­ داری کلسترول زرده را کاهش دادند (P<0.05). نتایج نشان داد که چای ترش می­ تواند در طول دوره اوج تولید، عملکرد تخمگذاری و ویژگی ­های کیفی تخم ­مرغ را بهبود بخشد و کلسترول زرده تخم ­مرغ را کاهش دهد. اثرات سودمند چای ترش بایستی در شرایط تولید تجاری بیشتر مورد بررسی قرار گیرد. Manuscript profile -
Open Access Article
38 - اثر زمانهای مختلف تغذیه روی عملکرد گاوهای شیری دورگه در طی تنش گرمایی تابستان
م. گول آی. احمد ر. خان اس. ام سهیل اس. اختر آ. رحمان آ. ایجاز آ. گوهربخش پرورش حیوانات اهلی در پاکستان یک موقعیت بی­نظیر را در توسعه اقتصاد ملی این کشور شامل می­شود. مطالعه حاضر برای ارزیابی به کارگیری استراتژی کاهش استرس گرما از طریق استفاده از زمان­های مختلف تغذیه روی صفات عملکردی تولید و ترکیب شیر و خصوصیات فیزیکوشیمیایی آ Moreبخش پرورش حیوانات اهلی در پاکستان یک موقعیت بی­نظیر را در توسعه اقتصاد ملی این کشور شامل می­شود. مطالعه حاضر برای ارزیابی به کارگیری استراتژی کاهش استرس گرما از طریق استفاده از زمان­های مختلف تغذیه روی صفات عملکردی تولید و ترکیب شیر و خصوصیات فیزیکوشیمیایی آن در گاوهای شیری در طی فصل تابستان انجام شد. بیست رأس گاو هلشتاین فریزین به طور تصادفی در چهار گروه تیماری (G1: 80/20، G2: 50/50،G3 : 100/0 وG4 : 0/100؛ از نسبت­های روز به شب) تقسیم شدند. تولید و ترکیب شیر و خصوصیات فیزیکوشیمایی آن و سطح مالون­دی­آلدئید در سرم خون تعیین شدند. نتیجه­گیری اینکه تغییرات در زمان تغذیه، به طور معنی­داری تولید شیر را در گروه­هایی که در بخش خنک­تر روز تغذیه می­کردند افزایش داد. ترکیب شیر و خصوصیات فیزیکوشیمیایی آن نیز از نوسانات تغذیه در زمان­های صبح و عصر تأثیر ­پذیرفته­اند. بنابراین، تغییر در زمان تغذیه در طی فصل تابستان در نواحی گرم یک روش عملیاتی مناسب برای بر طرف نمودن اثرات تنش گرمایی روی عملکرد تولیدی گاوهای شیری است. Manuscript profile -
Open Access Article
39 - Insulin Like Growth Factor-1 Fortification Improves Post Thaw Sperm Quality and Fertility of Goat
K. Sharma R. Ranjan S. Gupta -
Open Access Article
40 - اثر مکملهای دانههای خرد شده سیاهدانه و زیره سیاه بر عملکرد رشد، وضعیت آنتیاکسیدانی، فراسنجههای خونی و پاسخهای فیزیولوژیکی برههای سنجابی
س. کاکی م.م. معینی ف. هژبری ز. نیکوصفتهدف از انجام این آزمایش بررسی اثر مکملهای سیاهدانه و زیره سیاه بر عملکرد، وضعیت آنتیاکسیدانی، فراسنجههای خونی و پاسخهای فیزیولوژیکی برههای سنجابی بود. هیجده بره نرسنجابی (18-20 هفتهای) به صورت تصادفی به سه جیره آزمایشی تقسیم شدند: 1) جیره پایه به عنوان شاهد؛ 2) ج Moreهدف از انجام این آزمایش بررسی اثر مکملهای سیاهدانه و زیره سیاه بر عملکرد، وضعیت آنتیاکسیدانی، فراسنجههای خونی و پاسخهای فیزیولوژیکی برههای سنجابی بود. هیجده بره نرسنجابی (18-20 هفتهای) به صورت تصادفی به سه جیره آزمایشی تقسیم شدند: 1) جیره پایه به عنوان شاهد؛ 2) جیره پایه بعلاوه 30 گرم سیاهدانه به ازای هرکیلوگرم ماده خشک جیره و 3) جیره پایه بعلاوه 30 گرم زیره سیاه به ازای هرکیلوگرم ماده خشک جیره. برههای تغذیه شده با زیره سیاه وزن نهایی بالاتری نسبت به گروه شاهد داشتند (05/0>P). بره­های تغذیه شده با سیاهدانه در مقایسه باگروه شاهد غلظت آلانینآمینوترانسفراز و کلسترول کمتری داشتند (05/0>P). غلظت مالوندیآلدئید سرم خون برههای تغذیه شده با زیره سیاه در مقایسه با گروه شاهد کمتر بود (05/0>P). با توجه به نتایج این آزمایش افزودن مکمل سیاهدانه و زیره سیاه به جیره برههای پرواری میتواند سبب بهبود عملکرد رشد و فراسنجههای آنتیاکسیدانی شود. Manuscript profile -
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41 - The Effect of Apricot Tree Gum on the Quality of Frozen and Melted Ram Sperm in the Breeding Season
P. Khanzadeh G. Maghaddam H. Daghighkia S.A. Rafat R. Moradi -
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42 - The physiological and biochemical responses of directly seeded and transplanted maize (Zea mays L.) supplied with plant growth-promoting rhizobacteria (PGPR) under water stress
Saeed Rezazadeh Mohammadnabi ilkaee Fayaz Aghayari Farzad Paknejad Mehdi Rezaee -
Open Access Article
43 - Physiological responses of peppermint (Mentha piperita L.) to plant growth regulators and salinity stress
Ammar Fathi Meysam Oveysi Mohammad Nasri Hamidreza Tohidi Pourang Kasraei -
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44 - Morphological and physiological characteristics for evaluation of salicylic acid effects on Celosia argentea L. under salinity stress
Afsaneh Gholamzadeh Alam Sadegh Mousavi-Fard Abdolhossein Rezaei Nejad -
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45 - Physiological and biochemical changes of Alfalfa (Medicago sativa L.) cultivars during different growth stages
Alireza Khedri Behzad Sani behnam Zand Hamid Mozafari Payam Moavni -
Open Access Article
46 - Effect of calcium chloride and salicylic acid on antioxidative responses of lentils (Lens culinaris Medik.) under salt stress
Kobra Mahdavian -
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47 - Peroxide isozymes and Malondialdehyde content and ascorbate peroxidase activity in Berberis integerrima and Cercis siliquastrum under Cd-induced stress .
Leila Hakimi Esmail Khosropour -
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48 - Biological removal of cadmium from soil by phytoremediation and its impact on growth parameters, photosynthetic pigments, phenol and malondealdehyde content in Vetiveria zizianoides.
Azhir Khalil Aria Hossein Abbaspour Sekineh Saeidi Sar Mohsen Dehghani Ghanatghestani -
Open Access Article
49 - Effect of chitosan on antioxidant enzyme activity, proline, and malondialdehyde content in Triticum aestivum L. and Zea maize L. under salt stress condition
Lida Shams Peykani mozhgan farzami sepehr -
Open Access Article
50 - تاثیر بکارگیری اسپرمین آزاد خارجی روی عمر گلجایی رز شاخه بریده رقم ’دلس ویتا‘
Hamideh Ghorbani اصغر ابراهیم زاده Bagher Eftekhari Sis Mohammad Bagher Hasanpouraghadmپیری بخش جدایی ناپذیر چرخه نمو گیاهان می­باشد و می­تواند در سطح سلول، بافت و اندام اثرگذار باشد. پژوهش حاضر به­منظور مطالعه کارایی تیمار پس از برداشتی پلی آمین آزاد اسپرمین به عنوان یک ترکیب ضد پیری جهت افزایش عمر گلجایی گل­های شاخه بریده رز انجام شد. تی Moreپیری بخش جدایی ناپذیر چرخه نمو گیاهان می­باشد و می­تواند در سطح سلول، بافت و اندام اثرگذار باشد. پژوهش حاضر به­منظور مطالعه کارایی تیمار پس از برداشتی پلی آمین آزاد اسپرمین به عنوان یک ترکیب ضد پیری جهت افزایش عمر گلجایی گل­های شاخه بریده رز انجام شد. تیمار اسپرمین در غلظت­های صفر، ۱، ۲ و ۴ میلی­مولار به کار رفت. ویژگی­های مورفولوژیکی، بیوشیمیایی و فیزیولوژیکی گل­های رز رقم ’دلس ویتا‘ همچون قطر گل، جذب آب، وزن تر نسبی، نشت الکترولیتی، میزان مالون دی آلدهید، فعالیت آنزیم گایاکول پراکسیداز، میزان پراکسید هیدروژن و عمر گلجایی مورد ارزیابی قرار گرفتند. تاثیر کاربرد پلی آمین به صورت تیمار کوتاه مدت (پالس) بر روی صفات ذکر شده معنی­دار بوده و تیمار توسط اسپرمین خصوصا با کاربرد تیمار در غلظت ۴ میلی­مولار به طور مؤثری فرآیند پیری را در گل­های رز به تعویق انداخت. تیمار پلی آمین به واسطه­ی خاصیت کاتیونی و آنتی پراکسیداتیو خود منتج به کاهش قابل­توجهی در نشت الکترولیت گلبرگ­ها گشت. علاوه بر خاصیت ضد اتیلنی پلی­آمین­ها ویژگی کاتیونی بودن آن­ها نیز ممکن است که در تعیین عمر گلجایی گل­های شاخه بریده نقش مهمی داشته باشد. Manuscript profile -
Open Access Article
51 - NanoTiO2, Quantum dot-Graphene Oxide, and CeO2 Foliar Prescription Meliorates Growth and Some Physiological Traits of Gazania (Gazania splendens L.) Under Salinity
Lamia Vojodi Mehrabani Asghar Ebrahimzadeh Mina Tontab Ghadimi Mohammad Bagher Hassanpouraghdam Farzad RasoliThe production and maintenance of ornamental plants are closely related to the high consumption of fresh water. Today, due to the limited water resources, we have to water plants with salt water sources. To evaluate the effects of foliar application of nano TiO2, CeO2, MoreThe production and maintenance of ornamental plants are closely related to the high consumption of fresh water. Today, due to the limited water resources, we have to water plants with salt water sources. To evaluate the effects of foliar application of nano TiO2, CeO2, and quantum dot-graphene oxide (zero and 1.5 mg/L) and NaCl salinity stress (0, 75, 150 mM) on Gazania splendens L.; a factorial experiment was conducted based on completely randomized design. The results revealed that plant dry weight, flower number, proline and flavonoids content, antioxidant enzymes activity, MDA, H2O2, Na, N, and P content were influenced by the interaction effects of experimental treatments. The highest leaf dry weight, flower number, and N content were recorded at no-salinity × quantum dot-graphene oxide. The highest data for Na content, ion leakage (56.6%), H2O2 (246 nmol/mg FW), malondialdehyde (37 nmol/mg FW), and proline (1.1nmol/mg FW) content were recorded at NaCl150 mM × no-foliar spray. 150 mM salinity stress × quantum dot-graphene oxide increased catalase activity (8.9 µmol/g FW) in the plant. Superoxide dismutase and ascorbate peroxidase activity were influenced by NaCl150 mM × quantum dot-graphene oxide and TiO2 foliar spray. Chlorophyll index, total phenolics, and K/Na ratio were responded to the simple effects of salinity and foliar application. The top ratio of K/Na and chlorophyll index was recorded at quantum dot-graphene oxide foliar spray. 75 and 150 mM salinity improved phenolics content in plants. Foliar spray with all nanoparticles increased phenolics content. The overall results showed that salinity had adverse effects on the growth and physiological characteristics of Gazania splendens. Foliar treatments under 150 mM salinity stress; promisingly influenced the antioxidant enzymes activity and root dry weight of plants. All in all, Gazania splendens can tolerate up to 75 mM NaCl salinity stress without a remarkable decline in growth and physiological attributes. Manuscript profile -
Open Access Article
52 - The interaction between irrigation interval with manure and vermicompost on vegetative characteristics and yield of Lemon Verbena (Lippia citriodora L.)
Zahra Shadkam1 Farhad Mohajeri 2*This experiment was carried out to study the interaction between irrigation intervals with manures and vermicompost on vegetative characteristics and yield of Lemon Verbena a factorial experiment based on completely randomized design in Fasa, Iran during 2017. The first MoreThis experiment was carried out to study the interaction between irrigation intervals with manures and vermicompost on vegetative characteristics and yield of Lemon Verbena a factorial experiment based on completely randomized design in Fasa, Iran during 2017. The first factor involved irrigation intervals at four levels including two, four, six and eight days. The second factor was the utilization of vermicompost and/or livestock manure in four levels consisted of a control test, five percent manure (by weight), five percent vermicompost and 2.5% manure + 2.5% vermicompost which were added to the studied soil in pots. Water stress caused a significant decrease in the leaf relative water content and chlorophyll index of lemon verbena. The lowest ion leakage was obtained in vermicompost and manure and the highest amount of malondialdehyde in control test. In general, the results of this study showed that with increasing in drought stress a negative effect was observed on growth of Lemon Verbena which finally led to the reduction of biological yield. Also, the utilization of vermicompost and/or manure fertilizer have a positive effect on plant growth and improved plant's growth characteristics. Manuscript profile -
Open Access Article
53 - The residual effects of sodium carbonate of irrigation water on yield and chemical markers activity in autumn barley cultivars.
Mohammadreza DadniaTo evaluate the residual effects of sodium carbonate of irrigation water on barley (Hordeum vulgare) and chemical markers activity, an experiment was carried out in research field of Karaj Islamic Azad University in 2013 with split plot based on Completely randomize blo MoreTo evaluate the residual effects of sodium carbonate of irrigation water on barley (Hordeum vulgare) and chemical markers activity, an experiment was carried out in research field of Karaj Islamic Azad University in 2013 with split plot based on Completely randomize blocks design with four replications. In this experiment barely cultivars including Valfajr and Aras were in main plot and different rates of sodium carbonate of irrigation water in four levels (control,1, 2 and 3 meq lit–1) were in sub plot. In this experiment the traits such as grain yield, 1000 seed weight and the rate of chemical markers such as malon dialdehyde, di tyrosine and di hydroxy guanosine were assayed. The results showed that there was significant difference between sodium carbonate of irrigation water and cultivar treatments at 1% level also mean comparison represent decreased about 41.3% of grain yield with affected by high rates of sodium carbonate in comparison with control. The results also revealed that the level activity of chemical markers was highly affected by sodium carbonate of irrigation water and caused increasing them. In general, Valfajr identified as more resistant cultivar to sodium of irrigation water. Moreover, the rate of chemical markers can be used in selected resistant cultivars to salinity of irrigation water. Manuscript profile -
Open Access Article
54 - Effects of salicylic acid and jasmonic acid on some physiological and biochemical characteristics of marigold (Calendula officinalis L.) under salinity stress
M. Baniyaghoub Abkenar A. PazokiIn order to evaluate the effects of various concentrations of salicylic and jasmonic acid on root dry weight, shoot dry weight, contents of chlorophylls, carotenoids, anthocyanin, soluble sugars, proline, and malondialdehyde, percentage and weight of flower extract of m MoreIn order to evaluate the effects of various concentrations of salicylic and jasmonic acid on root dry weight, shoot dry weight, contents of chlorophylls, carotenoids, anthocyanin, soluble sugars, proline, and malondialdehyde, percentage and weight of flower extract of marigold plants under salinity stress, a factorial experiment was conducted in completely randomized design with four replications at research greenhouse of Islamic Azad University, Shahre-Rey Branch. The treatments were salinity at four levels With irrigation water including (0, 25, 50 and 75 mM) NaCl, Spraying salicylic acid in two levels (0, 0.7 mM) and, jasmonic acid in two levels (0, 100 µM). The results showed that the root dry weight, aerial dry weight, contents of chlorophylls, carotenoids, anthocyanin in the leaves and weight of flower extract were significantly decreased by increasing the severity of salinity. It was also found that the levels of soluble sugars, proline, malondialdehyde and flower extract percentage were significantly increased by increase in salinity levels. It was also observed that application of salicylic and jasmonic acid led to increase in root dry weight (72%), shoot dry weight (59%) and weight of flower extract (43%) that Somewhat reduce the negative effects of salinity. Manuscript profile -
Open Access Article
55 - The evaluation of antioxidant enzymes role in seed yield of some wheat (Triticum aestivum L.) genotypes under drought stress at post anthesis
P. Sharifi N. MohammadkhaniIn order to study the effects of drought stress on antioxidant enzymes activity, lipid peroxidation, chlorophyll content and grain yield of wheat cultivars, an experiment was conducted with a factorial arrangement in randomized complete block design with three replicate MoreIn order to study the effects of drought stress on antioxidant enzymes activity, lipid peroxidation, chlorophyll content and grain yield of wheat cultivars, an experiment was conducted with a factorial arrangement in randomized complete block design with three replicates in field of Agricultural Dryland Research Station, Maragheh, Iran. Six wheat genotypes and four irrigation treatments (control, 7, 17 and 23 days after anthesis) were used in this study. Genotypes were included Azar-2 and Sardari (Drought tolerant), HN7 and DH-2049 (moderately tolerant), and Sara and TEVEE (drought sensitive). Results showed that drought stress at different times after anthesis led to decrease in relative water content (on average from 82.5% to 53.5%) and chlorophyll content (on average from 12.33% to 3.17%) in flag leaves of different wheat genotypes. Activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) were significantly increased under drought conditions. It was also observed that tolerant genotypes which had lower MDA and RWC content and higher chlorophyll contents in drought conditions also showed the higher antioxidant enzymes activity than sensitive genotypes. It seems that lower antioxidant enzymes activity in sensitive genotypes caused a decrease in drought tolerance and therefore higher yield reduction in these genotypes (on average from 3150.23 kg per hectare to 1400.21 kg per hectare, 55.55% decrease) compare to tolerant genotypes (on average from 2800.72 kg per hectare to 1850.69 kg per hectare, 33.92% decrease). Manuscript profile -
Open Access Article
56 - تغییرات پارامترهای بیوشیمیایی در سگ های آلوده به بابزیا کانیس در ارومیه، ایران
کاوه عظیم زاده پریسا محمدی صفتهدف از این مطالعه بررسی تغییرات پارامترهای بیوشیمیایی در سگ های آلوده به بابزیوز در سه سطح بود. جنس بابزیا به عنوان یک انگل خونی بیماری زا باعث ایجاد مشکلات مالی در صنعت دامپروری می شود. بسیاری از مقالات منتشر شده پارامترهای بیوشیمیایی ناشی از بابزیوز را پیشنهاد کرده ان Moreهدف از این مطالعه بررسی تغییرات پارامترهای بیوشیمیایی در سگ های آلوده به بابزیوز در سه سطح بود. جنس بابزیا به عنوان یک انگل خونی بیماری زا باعث ایجاد مشکلات مالی در صنعت دامپروری می شود. بسیاری از مقالات منتشر شده پارامترهای بیوشیمیایی ناشی از بابزیوز را پیشنهاد کرده اند، اما هیچ مطالعه ای این پارامترها را در چهار سطح از پارازیتمی (کمتر از 1٪، 1٪، 2٪ و 3٪) تعیین نکرده است. سگ های مبتلا به بابزیوز بر اساس علائم بالینی و مشاهده گلبول های قرمز شناسایی شدند. پس از خونگیری از 38 سگ آلوده و 38 سگ سالم، پارامترهای بیوشیمیایی گلوکز پاراکسوناز، سوپراکسید دیسموتاز، کاتالاز، گلوتاتیون پراکسیداز و آدنوزین دآمیناز در پلاسما اندازه گیری شد. نتایج حاکی از افزایش و کاهش معنی داری در پارامترهای بیوشیمیایی بود. در نتیجه، نتایج نشان داد که بابزیوز سگ باعث آسیب سلولی، به ویژه در کلیه و کم خونی در سگهای آلوده میشود. علاوه بر این، بابزیوز باعث صدمات کلیه شد. این عفونت بر فعالیت آنزیم های کبدی تأثیر می گذارد.. نتایج بهدستآمده نشاندهنده افزایش بسیار معنیدار آسپارتات آمینوترانسفراز و آلانین آمینوترانسفراز سرم بود. Manuscript profile -
Open Access Article
57 - The effect of lead and copper on some physiological traits in plant species of grass pea (Lathyrus sativus (
Mahtab Beladi Ali Kashani Davoud Habibi Farzad Pak nezhad Mahya GolshanSome plant species are tolerant to environmental adverse conditions so that with their functional mechanisms prevent more production of free radicals of oxygen or counter with produced free radicals of oxygen. This study was done to investigate the effect of lead and co MoreSome plant species are tolerant to environmental adverse conditions so that with their functional mechanisms prevent more production of free radicals of oxygen or counter with produced free radicals of oxygen. This study was done to investigate the effect of lead and copper on Lathyrus sativus in contaminated soils with these elements. To evaluate and recognize the study species ability against heavy metals lead and copper an experiment was carried out on Lathyrus sativus cultivar Zanjan in 2009. The experiment was carried out factorially as randomized complete design with four levels of lead Pb (NO3)2 (0, 200, 400 and 800 mg/kg) and four levels of copper Cu (So4)2 (0, 150, 300, 450 mg/kg) were used.Although the results suggested the decrease in total chlorophyll content and lipid membrane under the toxicity of lead and copper, the more activity of the enzyme Superoxide dismutase (SOD) along with the increase in the levels of lead and copper in thid species prevented the more production of free radicals of oxygen in the study species, so that a negative coloration was observed between chlorophyll a, b and total chlorophyll and the enzyme superoxide dismutase (SOD). Also the coloration between Malondialdehyde (MDA) which is a means for measuring the lipid per oxidation process, and the enzyme Superoxide dismutase (SOD) was positive that means the increase in this biomarker content which is a sign of more production of oxygen for lipid membrane destruction, was accomplished with the more activity of enzyme Superoxide dismutase (SOD) for more digestion and elimination of destructive oxygen. Also the water condition in leaf tissues (RWC) was not effective by these elements. Manuscript profile -
Open Access Article
58 - Study of interaction of Titanium Dioxide nanoparticles and water management on weeds, physiological and biochemical characteristics of Corn (Zea mays L.)
E. Jalili F. Gangabadi M. S. Valiahdi M. Ghavami,This experiment was conducted in Karaj as split plot in a RCBD with 3 replications in 2017. The experimental treatments included irrigation management at three levels: irrigation with surface brush, sub surface (15 cm depth), and atmospheric and backyard in the main plo MoreThis experiment was conducted in Karaj as split plot in a RCBD with 3 replications in 2017. The experimental treatments included irrigation management at three levels: irrigation with surface brush, sub surface (15 cm depth), and atmospheric and backyard in the main plot and irrigation management at three levels: 50, 100 and 150 mm evaporation from the pan in subplots and application of titanium dioxide nanoparticles in three levels: zero (control), 2 and 4 mg/lit in sub-sub plots. The results of this study indicated that divergence method (sub surface type) can be used to optimize the use of water resources to reduce irrigation losses and achieve high yield in corn production. Application of irrigation management of subsurface ribbon barrels along with titanium dioxide nanoparticles of four milligrams per liter in corn fields reduced 85% of weeds. Result showded that corn and weed competition reduced tissue texture and allocated photosynthetic sources to seed more than stems and corn leaves. Also, the use of titanium dioxide nanoparticles increased the activity of antioxidant enzymes and corn yields. The highest yield in this treatment was obtained at 10450 kg/ha. Manuscript profile -
Open Access Article
59 - Evaluation of Oxidative Stress Biomarkers in Patients with Breast cancer Compared with Healthy Subjects
Hosnie Hoseini Masoud Kerman Saravi Shirin Shahraki Azade Sarani Afsane Sarabandi -
Open Access Article
60 - Exposure to Insecticide Mixture of Cypermethrin and Dichlorvos Induced Neurodegeneration by Reducing Antioxidant Capacity in Striatum
Princewill Udodi Tobechi ANONYE Damian Ezejindu Joshua ABUGU Chizubelu OMILE Ifechukwu Obiesie Roseline OGWO Chukwudumebi Abattam Darlington AKUKWU Godwin Uloneme Charles Oyinbo -
Open Access Article
61 - The Supplementation Role of Pumpkin Seed and Endurance Training on Oxidative Stress Syndrome of Fast-twitch Muscles in Male Rats Poisoned with Hydrogen Peroxide
Nahid Shokohirad Tahereh Bagherpour Nematollah Nemati Vida HojatiThe aim of this study was to investigate the role of pumpkin seeds and endurance training on oxidative stress syndrome in fast-twitch muscle tissue in male rats poisoned with hydrogen peroxide. For this purpose, a total of 48 adult male wistar rats weighing 220 ± MoreThe aim of this study was to investigate the role of pumpkin seeds and endurance training on oxidative stress syndrome in fast-twitch muscle tissue in male rats poisoned with hydrogen peroxide. For this purpose, a total of 48 adult male wistar rats weighing 220 ± 20 gr and at 8 weeks were administered in accordance with stress interventions (hydrogen peroxide), pumpkin seeds supplementation, and endurance training. In order to induce oxidative stress, intraperitoneal injection of H2O2 at a dose of 2 mmol/kg was performed three times a week every other day. Inoculation of pumpkin seeds was performed at 0.5 μg/day with intraperitoneal injection at a concentration of 300000 U/ml. The exercise groups daily exercised endurance training on a treadmill for 8 weeks, the endurance training protocol started at a speed of 8 m/min and the 10-degree gradient was started for 30 minutes on the treadmill in the first week and gradually increased to 20 m/min at an angle of 10 degrees for 60 minutes on the eighth week. For data analysis, repeated measures ANOVA and Bonferron's post hoc test were used at the level of =0.05. The results showed that training and receiving pumpkin seeds individually had significant effect on ATP concentration, ADP, ATP/ADP ratio, MDA and PAB of extensor finger muscle (fast-twitch) (p < /em> = 0.001). However, the interaction between training and pumpkin seeds had no significant effect on MDA (p < /em> = 0.56) and ATP/ADP ratio (p < /em> = 0.88), and extensor finger muscle (fast-twitch), but had significant effect on other variables (p < /em> = 0.001). Pumpkin seeds supplementation and endurance training can reduce oxidative stress in the body. Further research is required to be done on the supplementation of pumpkin seed and endurance training and its effects on oxidative stress. Manuscript profile -
Open Access Article
62 - Effects of Different Dietary Levels of Algae Chromochloris zofingiensis on Growth Performance and Meat Quality of Japanese Quail
Mohsen Mohamadisaei Houshang Lotfollahian Farzaneh Mehrabi Behrouz Yarahmadi Alireza Cheqeni Amin KazemizadehThe aim of this study was to determine the effect of different levels of Algae on performance and meat quality in Japanese quail. For this purpose, the numbers of 306 pieces of 3-days age of Japanese quail were used in a completely randomized design by; 6 experimental t MoreThe aim of this study was to determine the effect of different levels of Algae on performance and meat quality in Japanese quail. For this purpose, the numbers of 306 pieces of 3-days age of Japanese quail were used in a completely randomized design by; 6 experimental treatments, 3 replicates and 17 chickens per replication. The experimental treatments comprised different levels of Algae as; T1: no Algae (control group), T2; 0.05 %, T3: 0.1 %, T4: 0.15%, T5: 0.20 %and T5: 0.25% of this Algae was added to the basic diet. In addition to measuring yield performance at 35 days of age, 4 pieces of quail in both sexes (males and females) were randomly selected and slaughtered from each replicate for evaluation of meat quality. Our results showed that the effect of different levels of Algae in quail’s diets doesn’t have any significant effect on average body weight gaining, feed intake, feed conversion ratio and live weights (p > 0.05). Feeding different levels of Algae had significant effect on carcass weight (p < 0.05). Also, feeding other levels of Algae had significant effect on carcass yield rate (p < 0.05). Although, there was no significant difference between all treatments for meat malondialdehyde index (p > 0.05) But, it had significant effect on meat pH, water holding capacity and blood loss percentage between treatments (p < 0.05). In general, the results revealed that using Algae at level of % 0.1 of the diet could be effective for improving performance and meat quality in Japanese quail. Manuscript profile -
Open Access Article
63 - The Effect of Lycopene Supplement on Oxidative Stress and Total Antioxidant Capacity in Obese Men after Resistance Exercise
B. Hooshmand moghadam M. EskandariThe effect of supplementation with lycopene (tomato juice) on the TAC and MDA in obese men after resistance exercise was examined. In this study, 20 obese men (BMI ≥ 30) were randomly assigned to one of two groups: placebo (n = 10) and supplements (n = 10). Group sup MoreThe effect of supplementation with lycopene (tomato juice) on the TAC and MDA in obese men after resistance exercise was examined. In this study, 20 obese men (BMI ≥ 30) were randomly assigned to one of two groups: placebo (n = 10) and supplements (n = 10). Group supplements daily for two weeks 2 tablets of 200 mg lycopene and the placebo group the amount received placebo. Then the two groups participating in resistance exercise and venous blood samples at three stages: before supplementation (2 wk before the activity), before, and 24 h after the exercise was collected. The TAC and MDA concentration of biomarker in the supplement group were respectively increased and decreased significantly in the placebo group over time (P≤0.05). Supplementation with spirulina could increase plasma antioxidant capacity and reduce oxidative stress in obese men after resistance exercise. Manuscript profile -
Open Access Article
64 - Effect of Resveratrol Supplementation on Changes in Serum Levels of Ceruloplasmin and Malondialdehyde in Response to One Session of High Intensity Exercise in Sedentary Young Overweight Men
mohamad khoramdel najmeh rezaeianAbstractAntioxidants are first line of defense against the oxidative stress produced during high intensity exercise. Since, obesity is also related to imbalance of oxidative- antioxidative system of body; purpose of this study was to investigate the effect of 14 days of MoreAbstractAntioxidants are first line of defense against the oxidative stress produced during high intensity exercise. Since, obesity is also related to imbalance of oxidative- antioxidative system of body; purpose of this study was to investigate the effect of 14 days of resveratrol supplementation on changes in serum levels of ceruloplasmin and malondialdehyde in response to one bout of high intensity exercise in sedentary overweight young men. For this purpose, twenty sedentary overweight young men selected and randomly divided into two groups (experimental and control). Subjects in both experimental and control groups participated in one bout of exhaustive exercise (Bruce Test). Then, subjects in experimental group (supplementation) consumed one capsule of 400-mg resveratrol per day for 14 days. In control group (placebo), subject consumed one capsule of 400-mg lactose per day for 14 days. First blood sample was taken before the study and second one was done immediately after Bruce test. At the fifteenth day and at the end of 14 days of supplementation, subjects in both groups perform the exhaustive exercise again and forth blood sample was taken immediately following their performance. Data analysis was done using repeated measures ANOVA, and paired t-test, and P<0.05 was considered statistically significant. Finding showed 14 days of resveratrol supplementation had significant effect on serum levels of ceruloplasmin in response to one bout of high intensity exercise (P=0.043). It seems that 14 days of resveratrol supplementation has a significant effect on the improvement of function of antioxidant system of body in in sedentary overweight young men. Nevertheless, further research is necessary to understand the mediated mechanism. Manuscript profile -
Open Access Article
65 - Alterations of Serum Adenosine deaminase, Total Sialic acid, Malondialdehyde and Heat Shock Protein-27 in Sheep with Naturally Infected Liver Cysticercus taeniaculis
Kaveh Azimzadeh hooman azizizadehThe present study is involved investigation of total sialic acid (TSA), adenosine deaminase (ADA), malondialdehyde (MDA) and heat shock protein-27 (HSP-27) alterations in sheep during Cysticercus taeniaculis infection. For this purpose, 40 parasitized and 40 healthy she MoreThe present study is involved investigation of total sialic acid (TSA), adenosine deaminase (ADA), malondialdehyde (MDA) and heat shock protein-27 (HSP-27) alterations in sheep during Cysticercus taeniaculis infection. For this purpose, 40 parasitized and 40 healthy sheep were selected based on observation of cystic form in liver and lack of blood parasite along with no cystic conformation in carcass respectively. Thereupon, ten milliliters of blood were collected via the jugular vein from all ones and transferred to EDTA-contained and non-EDTA tubes and after preparation of plasma and sera, all parameters were detected in groups. The results revealed a significant decrease (p Manuscript profile
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