The effect of different copper sulfate (CuSO4) concentrations on some physiological parameters of (Melissa officinalis L.)

Subject Areas :

salehe  naderi ¹ (دانشگاه زابل)
حمیده  خواجه ² (دانشگاه زابل)
حسن  احمدی ³ (دانشگاه زابل)

Received: 2016-03-09 Accepted : 2016-03-25 Published : 2015-04-21

Keywords: Copper, Malondialdehyde, Antioxidative enzymes, melissa,

Abstract :

Melissa (Melissa offcinalis L.), a member of a Lamiaceae family, is a medical plant. The main most important pharmacological properties can be soothin, strengthening nerve, carminative and memory booster. The presence of heavy metals is one of important environmental stresses that can reduce growth and production of types of reactive oxygen. In the present study, the effect of copper sulfate (CuSO4) on some physiological parameters of melissa was examined. So melissa was treated with different concentrations of copper sulfate (0, 10, 20, 30 mM) in 6-leaf stage. Then melissa was harvested after two weeks in order to examine malondialdehyde, gayacol peroxidase, peroxidase and poly phenol oxidase antioxidative enzymes activity. The results showed that when copper sulfate increased in comparison with control, the activity of lipid peroxidation (malondialdehyde) and antioxidant enzymes increased significantly. Totally, all used copper sulfate concentrations caused toxicity in melissa and it showed that the plant is sensitive to huge amount of copper. This study investigated the effect of copper sulfate on malondialdehyde, gayacol peroxidase, peroxidase and poly phenol oxidase antioxidative enzymes activity in melissa.