اثر عصاره آبی الکلی کاسبرگ و برگ چای ترش بر عملکرد، کیفیت تخم مرغ، سامانه ایمنی و تعادل اکسیدانی مرغ های تخمگذار

Subject Areas : Camel

ش.  ثابت سروستانی ¹ (Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran)
س.م.  حسینی ² (Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran)
س.ه.  فرهنگ فر ³ (Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran)

Received: 2020-06-03 Accepted : 2020-06-03 Published : 2020-06-01

Keywords: مالون دی آلدهید, چای ترش, کلسترول زرده, پوسته تخم مرغ,

Abstract :

این آزمایش با هدف بررسی اثر عصاره آبی-الکلی گیاه چای ترش، بر عملکرد، کیفیت تخم­ مرغ، سامانه ایمنی و تعادل اکسیدانی مرغ­ های تخمگذار در طول شرایط محیطی خنثی طراحی شد. 200 قطعه مرغ تخم­گذار سویه های-لاین W-36 (در سن 23 هفتگی) به صورت پنج تیمار آزمایشی با چهار تکرار و 10 پرنده در هر تکرار تقسیم شدند. پرندگان تیمار یک با جیره بر پایه ذرت-سویا بدون عصاره تغذیه شدند، در حالیکه، تیمارهای دو و سه به ترتیب، 300 و 700 میلی­گرم در کیلوگرم عصاره برگ (HSLE) و تیمارهای چهار و پنج به ترتیب، 300 و 700 میلی­گرم در کیلوگرم عصاره کاسبرگ­ (HSLE) چای ترش را دریافت کردند. داده­ های عملکرد شامل تولید تخم ­مرغ، مصرف خوراک، توده تخم­ مرغ تولیدی و ضریب تبدیل خوراک به طور هفتگی ثبت شدند. شاخص­ های کیفیت تخم ­مرغ در هر 28 روز از دوره آزمایش مورد بررسی قرار گرفتند. از هر تکرار دو پرنده انتخاب و برای تعیین سامانه ایمنی و MDA پلاسما خون­گیری شد. همچنین از دو زرده تخم ­مرغ در هر تکرار برای بررسی MDA، کلسترول و تری‌گلیسرید زرده استفاده شد. مقایسه نتایج نشان داد، HSCE در سطح 700 میلی­گرم بر کیلوگرم نسبت به گروه شاهد میزان تخمگذاری را در زمان اوج تولید (P<0.05) تا 8 درصد افزایش داد. با این حال، تعادل اکسیدانی و عملکرد ایمنی بین گروه­های آزمایشی متفاوت نبود. مقاومت پوسته، ضخامت پوسته، وزن پوسته و شاخص شکل تخم ­مرغ به وسیله تیمارها تحت تأثیر قرار گرفتند (P<0.05). مرغ­های دریافت­کننده 700 میلی­گرم بر کیلوگرم HSLE نسبت به گروه شاهد (44.74 در برابر 47.67) به ­طور معنی­ داری کلسترول زرده را کاهش دادند (P<0.05). نتایج نشان داد که چای ترش می­ تواند در طول دوره اوج تولید، عملکرد تخمگذاری و ویژگی ­های کیفی تخم ­مرغ را بهبود بخشد و کلسترول زرده تخم ­مرغ را کاهش دهد. اثرات سودمند چای ترش بایستی در شرایط تولید تجاری بیشتر مورد بررسی قرار گیرد.

References:

Al-Batshan H., Scheideler S., Black B., Garlich J. and Anderson K. (1994). Duodenal calcium uptake, femur ash, and eggshell quality decline with age and increase following molt. Poult. Sci. 73, 1590-1596.

Allan W. and Gough R. (1974). A standard haemagglutination inhibition test for Newcastle disease. (1). a comparison of macro and micro methods. Vet. Res. 95, 120-123.

AOAC. (2007). Official Methods of Analysis. 18^th Ed. Association of Official Analytical Chemists, Arlington, Washington, DC., USA.

Calderon V.M. and Jensen L.S. (1990). The requirement for sulfur amino acid by laying hens as influenced by the protein concentration. Poult. Sci. 69, 934-944.

Carvajal-Zarrabal O., Waliszewski S.M., Barradas-Dermitz D.M., Orta-Flores Z., Hayward-Jones P.M., Nolasco-Hipólito C., Angulo-Guerrero O., Sánchez-Ricaño R., Infanzón R.M. and Trujillo P.R. (2005). The consumption of Hibiscus sabdariffa dried calyx ethanolic extract reduced lipid profile in rats. Plant Foods Hum. Nutr. 60, 153-159.

Cheng T.K., Coon C.N. and Hamre M.L. (1990). Effect of environmental stress on the ascorbic acid requirement of laying hens. Poult. Sci. 69, 774-780.

Cherian G., Wolfe F. and Sim J. (1996). Dietary oils with added tocopherols, effects on egg or tissue tocopherols, fatty acids, and oxidative stability. Poult. Sci. 75, 423-431.

Chuah A.M., Lee Y.C., Yamaguchi T., Takamura H., Yin L.J. and Matoba T. (2008). Effect of cooking on the antioxidant properties of coloured peppers. Food Chem. 111, 20-28.

El-Ghalid O. (2009). Exogenous estradiol, blood profile, productive and reproductive performance of female japanese quails at different stages of production. Asian J. Poult. Sci. 3, 1-8.

Fakeye T. (2008). Toxicity and immunomodulatory activity of fractions of Hibiscus sabdariffa (family Malvaceae) in animal models. African J. Tradit. Complement. Altern. Med. 5, 394-398.

Fakeye T.O., Pal A., Bawankule D. and Khanuja S. (2008). Immunomodulatory effect of extracts of Hibiscus sabdariffa (family Malvaceae) in a mouse model. Phytother. Res. 22, 664-668.

Ginter E., Kubec F., Vozar J. and Bobek P. (1979). Natural hypocholesterolemic agent, pectin plus ascorbic acid. Int. J. Vitam. Nutr. Res. 49, 406-412.

Gross W. and Siegel H. (1983). Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27, 972-979.

Hirunpanich V., Utaipat A., Morales N.P., Bunyapraphatsara N., Sato H., Herunsalee A. and Suthisisang C. (2005). Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa (Roselle) in vitro using rat low-density lipoprotein (LDL). Biol. Pharm. Bull. 28, 481-484.

Hirunpanich V., Utaipat A., Morales N.P., Bunyapraphatsara N., Sato H., Herunsale A. and Suthisisang C. (2006). Hypocholesterolemic and antioxidant effects of aqueous extracts from the dried calyx of Hibiscus sabdariffa in hypercholesterolemic rats. J. Ethnopharmacol. 103, 252-260.

Kahraman Z. (2009). Herbal feed additive and their usage in laying hen diets. J. Poult. Res. 8, 34-41.

Keshavarz K. (1996). The effect of different levels of vitamin C and cholecalciferol with adequate or marginal levels of dietary calcium on performance and eggshell quality of laying hens. Poult. Sci. 75, 1227-1235.

Khan R., Naz S., Nikousefat Z., Selvaggi M., Laudadio V. and Tufarelli V. (2012). Effect of ascorbic acid in heat-stressed poultry. Worlds Poult. Sci. J. 68, 477-490.

Kim J.K., So H., Youn M.J., Kim H.J., Kim Y., Park C., Kim S.J., Ha Y.A., Chai K.Y., Kim S.M., Kim K.Y. and Park R. (2007). Hibiscus sabdariffa water extract inhibits the adipocyte differentiation through the PI3-K and MAPK pathway. J. Ethnopharmacol. 114, 260-267.

Kwari I., Igwebuike J., Mohammed I. and Diarra S. (2011). Growth, haematology and serum chemistry of broiler chickens fed raw or differently processed sorrel (Hibiscus sabdariffa) seed meal in a semi-arid environment. Int. J. Sci. Nat. 2, 22-27.

Lin H.H., Chen J.H. and Wang C.J. (2011). Chemopreventive properties and molecular mechanisms of the bioactive compounds in Hibiscus sabdariffa. Curr. Med. Chem. 18, 1245-1254.

Lubega A.M., Bbosa G.S., Musisi N., Erume J. and Ogwal-Okeng J. (2013). Effect of the total crude extracts of Hibiscus sabdariffa on the immune system in the Wistar albino rats. African J. Pharm. Pharmacol. 7, 1942-1949.

Luhman C.M., Miller B.G. and Beitz D.C. (1990). Research note, the effect of feeding lovastatin and colestipol on production and cholesterol content of eggs. Poult. Sci. 69, 852-855.

McReynolds J.L., Genovese K.J., He H., Swaggerty C.L., Byrd J.A., Ricke S.C., Nisbet D.J. and Kogut M.H. (2009). Alfalfa as a nutritive modulator in maintaining the innate immune response during the molting process. J. Appl. Poult. Res. 18, 410-417.

McFarlane J.M. and Curtis S.E. (1989). Multiple concurrent stressors in chicks. 3. effects on plasma corticosterone and the heterophil, lymphocyte ratio. Poult. Sci. 68, 522-527.

Miller N.J., Rice-Evans C., Davies M.J., Gopinathan V. and Milner A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin. Sci. 84, 407-412.

Minka N., Fayomi A. and Ayo J. (2007). Protective influence calyces of Hibiscus sabdariffa against heat stress in laying hens during the hot-dry season. Res. J. Poult. Sci. 1, 7-11.

Morton J. (1987). Roselle. Pp. 281-286 in Fruits of Warm Climates. J.F. Morton, Ed. Creative Resource Systems Inc., Miami, Florida.

Mungole A. and Chaturvedi A. (2011). Hibiscus sabdariffa a rich source of secondary metabolites. Int. J. Pharm. Sci. Rev. Res. 6, 83-87.

Ni Y., Zhu Q., Zhou Z., Grossmann R., Chen J. and Zhao R. (2007). Effect of dietary daidzein on egg production, shell quality, and gene expression of ER-α, GH-R, and IGF-IR in shell glands of laying hens. J. Agric. Food Chem. 55, 6997-7001.

North M.O. (1984). Commercial Chicken Production Manual. The Avi Publishing Company Inc., Dawsonville, Georgia.

Ochani P.C. and Mello P.D. (2009). Antioxidant and antihyperlipidemic activity of Hibiscus sabdariffa leaves and calyces extracts in rats. Indian J. Exp. Biol. 47, 276-282.

Okoko T. and Ere D. (2012). Hibiscus sabdariffa extractivities on cadmium-mediated alterations of human U937 cell viability and activation. Asian Pac. J. Trop. Med. 5, 33-36.

Olaleye M.T. (2007). Cytotoxicity and antibacterial activity of methanolic extract of Hibiscus sabdariffa. J. Med. Plants Res. 1, 9-13.

Olatunji L.A., Adebayo J.O., Oguntoye O.B., Olatunde N.O., Olatunji V.A. and Soladoye A.O. (2005). Effects of aqueous extracts of petals of red and green Hibiscus sabdariffa on plasma lipid and hematological variables in rats. Pharm. Biol. 43, 471-474.

Ologundudu A., Ologundudu A., Oluba O., Omotuyi I. and Obi F. (2010). Effect of Hibiscus sabdariffa anthocyanins on 2, 4-dinitrophenylhydrazine-induced tissue damage in rabbits. J. Toxicol. Environ. Health Sci. 2, 1-6.

Omotuyi I., Ologundudu A., Onwubiko V. and Wogu M. (2011). Hibiscus sabdariffa anthocyanins alter circulating reproductive hormones in rabbits. J. Diabetes Endocrinol. 1, 36-45.

Pandey K.B. and Rizvi S.I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell. Longev. 2, 270-278.

Picotto G., Massheimer V. and Boland R. (1996). Acute stimulation of intestinal cell calcium influx induced by 17β-estradiol via the cAMP messenger system. Mol. Cell. Endocrinol. 119, 129-134.

Radwan N.L., Hassan R., Qota E. and Fayek H. (2008). Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. Int. J. Poult. Sci. 7, 134-150.

Renard C.M., Watrelot A.A. and Le Bourvellec C. (2017). Interactions between polyphenols and polysaccharides: mechanisms and consequences in food processing and digestion. Trend. Food Sci. Technol. 60, 43-51.

Rice-Evans C.A. and Miller N.J. (1996). Antioxidant activities of flavonoids as bioactive components of food. Biochem. Soc. Trans. 24, 790-795.

Sabet Sarvestani S., Hosseini S.M. and Farhangfar H. (2019). The effect of sour of tea (Hibiscus sabdariffa) plant extract on immune system, plasma antioxidant status, oxidant balance and blood biochemical and functional parameters of old laying hens. Iranian J. Anim. Sci. Res. 12, 22-31.

Saeed I.A., Ali L., Jabeen A., Khasawneh M., Rizvi T.A. and AshrafS.S. (2013). Estrogenic activities of ten medicinal herbs from the middle east. J. Chromatogr. Sci. 51, 33-39.

Sahin K., Orhan C., Tuzcu M., Ali S., Sahin N. and Hayirli A. (2010). Epigallocatechin-3-gallate prevents lipid peroxidation and enhances antioxidant defense system via modulating hepatic nuclear transcription factors in heat-stressed quails. Poult. Sci. 89, 2251-2258.

Sahin K., Ozercan R., Onderci M., Sahin N., Gursu M.F., Khachik F., Sarkar F.H., Munkarah A., Ali-Fehmi R. and Kmak D. (2004). Lycopene supplementation prevents the development of spontaneous smooth muscle tumors of the oviduct in Japanese quail. Nutr. Cancer. 50, 181-189.

Sahin K., Sahin N., Onderci M., Yaralioglu S. and Kucuk O. (2001). Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress. Vet. Med. 46, 140-144.

Saki A.A., Rahmati M.M., Zamani P., Zaboli K. and Matin H.R. (2010). Can vitamin C elevate laying hen performance, egg and plasma characteristics under normal environmental temperature? Italian J. Anim. Sci. 9, 313-317.

SAS Institute. (2015). SAS^®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.

Smith W.S., Green C. and Jerry M. (2003). Determination of ascorbic acid (vitamin C) in commerical tablets by iodometric titration. MS Thesis. Stevens Institute of Technology, Hoboken, USA.

Sukkhavanit P., Angkanaporn K. and Kijparkorn S. (2011). Effect of roselle (Hibiscus sabdariffa) calyx in laying hen diet on egg production performance, egg quality and TBARS value in plasma and yolk. Thai J. Vet. Med. 41, 337-344.

Sullivan A.C., Hamilton J.G., Miller O.N. and Wheatley V.R. (1972). Inhibition of lipogenesis in rat liver by (−)-hydroxycitrate. Arch. Biochem. Biophys. 150, 183-190.

Swain T. (1965). Analytical methods for flavonoids. Pp. 543-544 in The Chemistry and Biochemistry of Plant Pigments. T.W. Goodwin, Ed. Academic Press, London, United Kingdom.

Tseng T.H., Kao E.S., Chu C.Y., Chou F.P., Wu H.W.L. and Wang C.J. (1997). Protective effects of dried flower extracts of Hibiscus sabdariffa against oxidative stress in rat primary hepatocytes. Food Chem. Toxicol. 35, 1159-1164.

Turkmen N., Sari F. and Velioglu Y.S. (2005). The effect of cooking methods on total phenolics and antioxidant activity of selected green vegetables. Food Chem. 93, 713-718.

Vanzo A., Terdoslavich M., Brandoni A., Torres A.M., Vrhovsek U. and Passamonti S. (2008). Uptake of grape anthocyanins into the rat kidney and the involvement of bilitranslocase. Mol. Nutr. FoodRes. 52, 1106-1116.

Wang H., Cao G. and Prior R.L. (1997). Oxygen radical absorbing capacity of anthocyanins. J. Agric. Food Chem. 45, 302-309.

Wang J., Cao X., Jiang H., Qi Y., Chin K.L. and Yue Y. (2014). Antioxidant activity of leaf extracts from different Hibiscus sabdariffa accessions and simultaneous determination five major antioxidant compounds by LC-Q-TOF-MS. Molecules. 19, 21226-21238.

Wang J., Yue H., Wu S., Zhang H. and Qi G. (2017). Nutritional modulation of health, egg quality and environmental pollution of the layers. Anim. Nutr. 3, 91-96.

Wong P.K., Yusof S., Ghazali H. and Che Man Y. (2002). Physico-chemical characteristics of roselle (Hibiscus sabdariffa). Nutr. Food Sci. 32, 68-73.

Yoshioka T., Kawada K., Shimada T. and Mori M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am. J. Obstet. Gynecol. 135, 372-376.

Zhao R.Q., Zhou Y.C., Ni Y.D., Lu L.Z., Tao Z.R., Chen W.H. and Chen J. (2005). Effect of daidzein on egg-laying performance in Shaoxing duck breeders during different stages of the egg production cycle. British Poult. Sci. 46, 175-181.

Zhou E., Pan X. and Tian X. (2009). Application study of xylo-oligosaccharide in layer production. Mod. Appl. Sci. 3, 103-107.