Effect of Aqueous-Alcoholic Extract of Hibiscus sabdariffa Calyx and Leaf Calyx and Leaf on Performance, Egg Quality, Immune System and Antioxidant Balance of Laying Hens
محورهای موضوعی : Camelش. ثابت سروستانی 1 , س.م. حسینی 2 , س.ه. فرهنگ فر 3
1 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Birjand, Birjand, Iran
کلید واژه: yolk cholesterol, Malondialdehyde, egg shell, <i>H. sabdariffa</i>,
چکیده مقاله :
This experiment was conducted to obtain information on how aqueous-alcoholic extractof H. sabdariffa plant can affect laying hen performance, egg quality, immune system and antioxidant balance during thermo-neutrals conditions. Two hundred hens (23-wk-old) were divided into 5 experimental treatments with 4 replicates each, 10 white-egg Hy-Line (W36) hens per cage. Treatment 1 (control) birds were fed with a corn and soybean meal-based diet without extract, whereas treatments 2 and 3 received, 300 and 700 mg/kg of H. sabdariffa leaf extract (HSLE) and treatments 4 and 5, received 300 and 700 mg/kg of H. sabdariffa calyx extract (HSCE), respectively. The weekly recorded performance data included egg production, feed intake, egg mass and feed conversion ratio. The egg quality parameters were evaluated at each 28 days of the experimental periods. Two hens from each replicate were selected and the blood was gathered to determine the immune system and plasma malondialdehyde (MDA). Also, two egg yolks from each replicate were used to investigated yolk MDA, cholesterol and triglyceride. Comparing the results with the control showed, HSCE at 700 mg/kg promoted laying rate during peak production (p <0.05) up to 8%. However, antioxidant balance and immune function did not differ between treatment groups. Eggshell strength, eggshell thickness, eggshell weight and egg-shape index were affected by treatments (p <0.05). Hens receiving 700 mg/kg of HSLE significantly decreased yolk cholesterol (p <0.05) compared to the control group (44.74 vs. 47.67). The results suggest that the H. sabdariffa can improve laying performance, egg qualitative characteristics and reduce egg yolk cholesterol during peak production period. The beneficial effects of the H. sabdariffa should be further studied in commercial production conditions.
این آزمایش با هدف بررسی اثر عصاره آبی-الکلی گیاه چای ترش، بر عملکرد، کیفیت تخم مرغ، سامانه ایمنی و تعادل اکسیدانی مرغ های تخمگذار در طول شرایط محیطی خنثی طراحی شد. 200 قطعه مرغ تخمگذار سویه های-لاین W-36 (در سن 23 هفتگی) به صورت پنج تیمار آزمایشی با چهار تکرار و 10 پرنده در هر تکرار تقسیم شدند. پرندگان تیمار یک با جیره بر پایه ذرت-سویا بدون عصاره تغذیه شدند، در حالیکه، تیمارهای دو و سه به ترتیب، 300 و 700 میلیگرم در کیلوگرم عصاره برگ (HSLE) و تیمارهای چهار و پنج به ترتیب، 300 و 700 میلیگرم در کیلوگرم عصاره کاسبرگ (HSLE) چای ترش را دریافت کردند. داده های عملکرد شامل تولید تخم مرغ، مصرف خوراک، توده تخم مرغ تولیدی و ضریب تبدیل خوراک به طور هفتگی ثبت شدند. شاخص های کیفیت تخم مرغ در هر 28 روز از دوره آزمایش مورد بررسی قرار گرفتند. از هر تکرار دو پرنده انتخاب و برای تعیین سامانه ایمنی و MDA پلاسما خونگیری شد. همچنین از دو زرده تخم مرغ در هر تکرار برای بررسی MDA، کلسترول و تری‌گلیسرید زرده استفاده شد. مقایسه نتایج نشان داد، HSCE در سطح 700 میلیگرم بر کیلوگرم نسبت به گروه شاهد میزان تخمگذاری را در زمان اوج تولید (P<0.05) تا 8 درصد افزایش داد. با این حال، تعادل اکسیدانی و عملکرد ایمنی بین گروههای آزمایشی متفاوت نبود. مقاومت پوسته، ضخامت پوسته، وزن پوسته و شاخص شکل تخم مرغ به وسیله تیمارها تحت تأثیر قرار گرفتند (P<0.05). مرغهای دریافتکننده 700 میلیگرم بر کیلوگرم HSLE نسبت به گروه شاهد (44.74 در برابر 47.67) به طور معنی داری کلسترول زرده را کاهش دادند (P<0.05). نتایج نشان داد که چای ترش می تواند در طول دوره اوج تولید، عملکرد تخمگذاری و ویژگی های کیفی تخم مرغ را بهبود بخشد و کلسترول زرده تخم مرغ را کاهش دهد. اثرات سودمند چای ترش بایستی در شرایط تولید تجاری بیشتر مورد بررسی قرار گیرد.
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