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Open Access Article
1 - Serum levels of malondialdehyde, glutathione peroxidase and superoxide dismutase in patients with non-Hodgkin's lymphoma in Zahedan
Hosnie Hoseini Parichehreh Yaghmaei Gholamreza Bahari Saeed Aminzadeh -
Open Access Article
2 - A Study on the Antibacterial Activity of Lactic Acid Bacteria Isolated from Traditional Iranian Milk Samples
P. Taheri N. Samadi M. R. Khoshayand M. R. Fazeli H. Jamalifar M.R. Ehsani -
Open Access Article
3 - Experimental and theoretical studies on green and efficient deoximation using H202 catalyzed by Montmorillonite-K10 supported MnC12
M. Mahmoodi Hashemi A. Ezabadi Gh.R. Najafi F. Mollaamin M. Khaleghian R. Zhiani -
Open Access Article
4 - Experimental and theoretical studies on efficient regeneration of carbonyl compounds from oximes under green, mild and completely heterogeneous nanocatalysis
Gh.R. Najafi M. Saffari M. Masteri-Farahani F. Mollaamin -
Open Access Article
5 - The Effect of Foliar Application of Proline and Glycine-Betaine on Growth Indices, Proline Content and Enzymatic Activity of Aromatic Geranium (Pelargonium Graveolens) Under Low Water Stress
Majid Khodabakhsh Elham DanaeeBackground and Aim: Aromatic geranium is an ornamental perennial plant which its essential oil is used in perfumery, cosmetics, food and pharmaceutical industries. Water scarcity is one of the most important environmental stresses limiting plants’ growth and MoreBackground and Aim: Aromatic geranium is an ornamental perennial plant which its essential oil is used in perfumery, cosmetics, food and pharmaceutical industries. Water scarcity is one of the most important environmental stresses limiting plants’ growth and yield, which greatly impacts the morphophysiological, biochemical and enzymatic properties of plants and limits their development and survival. Using organic osmolytes such as proline and glycine-betaine increase the capacity and speed of photosynthesis, absorb ions such as magnesium and potassium, prevent the degradation of pigment-protein compounds, maintain plant pigments, strengthen the antioxidant system, and impact in inhibiting the adverse effects of water scarcity in plants. Thus, the aim of this study is to determine the best substance and effective concentration improving the quality and aromatic geraniums flowering under water scarcity stress.Methods: Aromatic geranium cuttings were kept in commercial greenhouses with a temperature of about 17 to 20 Cᵒ, 60 to 70% relative humidity and light intensity of about 50 to 60 µm/m2S in Karaj city. Feeding with Hoagland solution was done once a week and in order to measure the soil moisture, the potting system was used, and irrigation was carried out based on the soil weight change according to the determined field capacity (FC). Foliar spraying with proline and glycine betaine (0, 50 and, 100 mg/l) was applied for a month after two weeks of placing the cuttings in pots and 24 hours of being at different levels of low scarcity stress (25, 50, 75 and, 100% field capacity), sequently. After completing the treatments, sampling and evaluating of traits such as fresh and dry weight of shoots and roots, petals anthocyanin, total leaf chlorophyll, proline and activity of superoxide dismutase and peroxidase enzymes were performed. The experiment was performed in 2019 as a factorial experiment in a completely randomized statistical design with two factors of applying water scarcity stress and spraying with proline and glycine-betaine and their interaction. Then, data were analyzed using SPSS, the means were compared with Duncan's multiple domain and graphs were drawn in Excel.Results: Data means comparison showed that the highest fresh and dry weight of shoots with 67.25 and 8.53 g and the highest fresh and dry weight of roots with 15.62 and 3.45 g were observed in 100% FC (control) and the lowest shoot fresh and dry weight with 44.76 and 4.45 g and the lowest fresh and dry weight of roots with 10.57 and 1.17 g, were in 25% FC treatment (without foliar application). Also, the highest and lowest petals anthocyanin with 2.8925 and 1.5775 mg/g FW and total leaf chlorophyll with 15.3735 and 11.1632 mg/g FW were in 100% FC (control) and 25% FC treatment (without foliar application). The highest and lowest proline levels were 3.84 and 1.67 mg/g of FW in 25% FC treatment (without foliar application) and 75% FC treatment + 100 mg/l of proline. The highest activity of superoxide dismutase and peroxidase enzymes with 2.97 and 16.52 UE/g of FW in 75% FC + glycine-betaine 100 mg/l treatment and the lowest with 1.12 and 12.93 UE/g FW in 25% FC treatment (without foliar application), was obtained.Conclusion: The results demonstrate that the fresh and dry weight of shoots and roots decreased with increasing water scarcity stress, which this reduction was in 25% or more of field capacity and foliar application of plants with glycine betaine and proline inhibited the negative effects of stress at a concentration of 100 mg/l, both combinations were more visible. Petal anthocyanin and total leaf chlorophyll dropped with rising water scarcity stress and, treatment of plants with glycine betaine and proline had an effective role in ameliorating plant pigments. In addition, proline levels are increased by water scarcity stress. The highest enzyme activity was in 75% FC and with increasing water scarcity stress, enzyme activity reduced. Foliar application of plants with glycine betaine and 100 mg/l proline reduced the negative effect of water stress in 50 and 75% FC compared to 25% FC. Hence, according to the research results, the use of organic osmolytes such as glycine betaine and proline can be recommended to decrease the negative effects of water scarcity stress in ornamental plants. Manuscript profile -
Open Access Article
6 - Investigating the Effects of Salinity Stress Caused By Different Levels of Sodium Chloride (Nacl) and Potassium Chloride (Kcl) On the Growth and Quality of Sport Turfgrass
Elham Danaee Amir DoostmohamadiBackground and Aim: Grass is one of the most important cover plants in most green spaces, and considering that Iran is considered one of the dry and semi-arid regions of the world, water and soil salinity affects the growth of plants, as a result, using salt-resistant g MoreBackground and Aim: Grass is one of the most important cover plants in most green spaces, and considering that Iran is considered one of the dry and semi-arid regions of the world, water and soil salinity affects the growth of plants, as a result, using salt-resistant grass is one of the solutions to create green space in these areas. Therefore, the purpose of this research is to investigate the salinity tolerance of sport turfgrass caused by sodium chloride and potassium chloride salts. Method: In April, sports grass seeds were planted in an area located in Karaj province in the form of a r randomized complete block design with three replications in plots of 2 m2 (1 x 2 m2) and were cultivated with a density of 40 g m-2. The experimental treatments included sodium chloride and potassium chloride salts with concentrations of zero, 25, 50 and 75 mg l-1. After about 5 weeks of planting the seeds, salt stress was applied for one month through irrigation with salts twice a week and each time with 12 liter of salt solution and then sampling was done to evaluate fresh and dry weight of shoots and roots, total chlorophyll, proline, protein and activity of superoxide dismutase and peroxidase enzymes. Results: Data analysis showed that the treatments had a significant effect on the assessed traits. The highest fresh and dry weight of shoots (3.85-4.87 g) and roots (1.46-23.2 g) and total chlorophyll (16.96 mg g-1) were observed in the control, while the highest protein (3.73 μg mg-1) and activity of superoxide dismutase (4.62 enzyme units g-1) and peroxidase (4.12 enzyme units g-1) in 75 mg l-1 sodium chloride treatment and the highest proline content (12/ 8 mg g-1) was observed in 75 mg l-1 potassium chloride treatment, also the lowest fresh and dry weight of aerial parts (2.12-3.08 g) and total chlorophyll (12.53 mg g-1) in 75 mg l-1 potassium chloride treatment and the lowest fresh and dry weight of roots (0.59-0.96 g) were obtained in 75 mg l-1 sodium chloride treatment, and the lowest content of proline (4.48 mg g-1), protein (2.48 μg mg-1) and the activities of superoxide dismutase (3.00 enzyme units g-1) and peroxidase (2.93 enzyme units g-1) were in the control. Conclusion: According to the obtained results, sport turfgrass was able to tolerate stress conditions in 25 and 50 mg l-1 salt stress to some extent by increasing compounds such as proline, protein and antioxidant enzyme activity, but increasing the concentration of salts used, especially potassium chloride salt (75 mg l-1) had the greatest effect in reducing the vegetative traits and increasing the enzyme activity of sport turfgrass. Manuscript profile -
Open Access Article
7 - Evaluation of Antifungal and Antioxidant Properties of Fennel Extract
N. Noshirvani H. Fasihi E. NourmohammadiIntroduction: The recent literature search has introduced fennel and its extracts as potential products with excellent properties and characteristics. The aim of this investigation is to obtain fennel extracts and evaluate its antioxidant and antifungal activities. Mate MoreIntroduction: The recent literature search has introduced fennel and its extracts as potential products with excellent properties and characteristics. The aim of this investigation is to obtain fennel extracts and evaluate its antioxidant and antifungal activities. Materials and Methods: Water, acetone, methanol and ethanol were employed as solvents and extracts were obtained by soxhlet procedure. The amount of polyphenolic and flavonoid compounds of each extract was determined. In addition, the antioxidant activity of methanolic extract of fennel was evaluated based on DPPH radical scavenging activity. Afterwards, the effect of different concentrations of methanolic extract (0, 100, 250, 500 and 1000 ppm) and powder (500 and 1000 ppm) of fennel on the oxidantion activity of sunflower seed oil was investigated in terms of peroxide and thiobarbituric acid values during 15 days of incubation at 70 ⁰C. The antifungal activity of powder and extract of fennel against Aspergillus niger and Penicillium digitatum was also investigated by disc diffusion test. Results: According to the obtained results methanol represented the highest efficiency in extraction of phenolic compounds. The results indicated that fennel extract, especially at high concentrations, showed high DPPH radical-scavenging activity, however its effect was lower than that of TBHQ. The results of oxidation activity showed that the extract and powder of fennel, decreased the oxidation rate of sunflower seed oil as compared to the control. The results of microbiology test indicated good antifungal activity of fennel extract against the two tested microorganisms. Conclusion: Considering the results, fennel extract can be applied as a healthy plant source with good antioxidant and antifungal activities. Manuscript profile -
Open Access Article
8 - Simultaneous Effect of Temperature and Hydrogen Peroxid on Degradation of Anthocyanins in Three Berberis Species Juices
M. Farhadi Chitgar M. Varidi F. Shahidi A. TorabiIntroduction: Antocyanins provide a pleasant and attractive appearance in fruits and vegetables particularly fruit juices. Therefore it is necessary to study the effect of hydrogen peroxide (H2O2) as a widespread material in packaging sterilant in aseptic processing sys MoreIntroduction: Antocyanins provide a pleasant and attractive appearance in fruits and vegetables particularly fruit juices. Therefore it is necessary to study the effect of hydrogen peroxide (H2O2) as a widespread material in packaging sterilant in aseptic processing systems on anthocyanins from various juices. Materials and Methods: In this study, the simultaneous effect of hydrogen peroxide in five different concentrations and three different temperatures (10, 15, 20, 25 and 30 mmol/L) and (10, 20 and 30 ̊C) on degradation of anthocyanins in Berberis vulgaris, Berberis cratagina and Beberis integerrima juices have been investigated. Results: The degradation of anthocyanins in all treatments were fitted both zero and first order kinetics models. The results showed the degradation of anthocyanins in these three Berberis juices followed the first-order reaction kinetics. In most cases, the reaction rate constant (k) and half-life (t1/2) differed significantly among the three Berberis in various concentrations and temperatures (p<0.05). The difference between Berberis vulgaris juice and Berberis cratagina juice was lower. The reaction rate constant (k) increased and half-life (t1/2) decreased with increasing concentration of hydrogen peroxide in all juices. The temperature dependence of anthocyanins degradation was determined by calculating the activation energy (Ea) and temperature quotient (Q10). At the concentration of 10-20 mmol/L, Berberis vulgaris juice showed the lowest temperature dependence, whereas at higher concentrations (25 and 30 mmol/L) Beberis integerrima juice had the lowest temperature dependence. Conclusion: Anthocyanins from berberis species juices were found to be very susceptible to H2O2. Therefore, aseptic systems should be frequently controlled to ensure the effective removal of residual H2O2 from the food contact surfaces. Since the rate of anthocyanin degradation by H2O2 is highly dependent on temperature, cold storage of these juices is strongly recommended. Manuscript profile -
Open Access Article
9 - The Stabilizing Effect of Three Varieties of Crude Mango Seed Kernel Oil on Tallow
J Jafari M Gharachorloo M. H. Hemmaci -
Open Access Article
10 - The Effect of Different Concentrations of Activated Carbon with Bleaching Earth During Bleaching Process on Physicochemical Properties of Soybean Oil
N. Aliyar Zanjani Z. Piravi Vanak -
Open Access Article
11 - Optimization of Effective Parameters in Removal of Acid Blue 25 by Fenton Process with the Aim of Reducing Iron Consumption
Sobhan Hooshmand Bita AyatiBackground & Objective: Advanced oxidation processes (AOPs) are recognized as the most effective and capable available methods to remove and degrade different kind of dyes. Among various advanced oxidation processes, Fenton process is one of the most effective techn MoreBackground & Objective: Advanced oxidation processes (AOPs) are recognized as the most effective and capable available methods to remove and degrade different kind of dyes. Among various advanced oxidation processes, Fenton process is one of the most effective techniques that are used successfully to remove dyes without producing additional toxic by-products.Method: In the present study, all experiments were performed in a 500 mL rectangular plexiglass cubic reactor. The effect of different system parameters including pH, [Fe2+] to [H2O2] ratio, initial dye concentration, H2O2 concentration, stirrer velocity and temperature were examined and optimized using one- factor- at- a- time (OFAT) method.Findings: The results obtained from this study showed that Fenton process can remove 96 percent of Acid Blue 25 from aqueous solution. The optimal condition using Fenton process included initial concentration of 150 mg/l, pH= 3, molar ratio of [Fe2+]/[H2O2] = 0.3, stir. vel.= 100 rpm, temperature 43°C and 10 min contact time.Discussion and Conclusions: Fenton process is suggested as a suitable method for the removal of Acid Blue 25. Manuscript profile -
Open Access Article
12 - Decolorizing of Synthetic Textile Dyes by Phanerochaete Chrysosporium Immobilized on Mineral Igneous
Rasoul Ghasemzadeh Afzal Karimi Farzaneh VahabzadehThe white- rot fungus phanerochaete chrysosporium immobilized on the mineral igneous was grown in both stationary and agitated cultures using either carbon- or nitrogen-limited growth medium to study the ability of the fungus to degrade various types of textile dyes. Ki MoreThe white- rot fungus phanerochaete chrysosporium immobilized on the mineral igneous was grown in both stationary and agitated cultures using either carbon- or nitrogen-limited growth medium to study the ability of the fungus to degrade various types of textile dyes. Kiss iris immobilized white-rot fungi phanerochaete chrysosporium could completely decolorize 45 ppm of divers structural dye classes: azoic, reactive, acid, basic, disperse and metal-complex within 7-15 days. Except metal-complex the only decolorized by biological absorption, other classes of dyes degraded by lignins and manganese peroxides enzymes produced by the fungus. During the decolorizing process, activity of lignins and manganese peroxides enzymes was observed. Maximum activity of each of them, in the shaken and immobilized culture within 8 & 9 was 174 U/L and 500 U/L, respectively Manuscript profile -
Open Access Article
13 - The effect of "Hydrogen peroxide- Silver ion Complex" on fecal coliform content in aerated lagoon Effluent
Mohammad Khazaei Ramin Nabizadeh Kazem Naddafi Hasan Izanlou Zeinab Yavari Mehdi AsadiAn appropriate disinfectant in water and wastewater applications must have acceptable effect on different kind of micro-organisms especially in minor concentrations which should contain ignorable toxicity on human and animals and its environmental side effects must be v MoreAn appropriate disinfectant in water and wastewater applications must have acceptable effect on different kind of micro-organisms especially in minor concentrations which should contain ignorable toxicity on human and animals and its environmental side effects must be very low. Studies related to finding non-chlorinated disinfectants which have same effects of chlorine and not contain its adverse by-products are continued in recent years. Hydrogen peroxide solution with stabilized formulation and oligo-dynamic amounts of silver ion (trade mark: Nanosil) recently become a popular disinfectant for fruits، vegetables، surfaces، medical instruments and disinfections. This study was based on determining removal efficiency of fecal coliforms from samples taken from Qom wastewater treatment plant effluents after exposing with hydrogen peroxide-silver ion complex. Disinfectant efficiency was studied in six concentrations. Contact time for all concentrations was one hour. Sample size was 12 and sampling was daily. Results reveal that in concentration; 80mg/l، disinfectant achieves 2.1 logarithmic removals of fecal coliforms. In concentration; 480mg/l، fecal coliforms logarithmic removal was 5.3. In CT value 5800(mg/l). Min and more، the fecal coliforms effluent standard for surface water discharge and agriculture irrigation was achieved (Iranian environmental protection agency act). Use of hydrogen peroxide-silver ion complex as a water and wastewater disinfectant، has various benefits such as; don't remain hazardous by products، measurable residual amount an but because of its oxidation nature when contact the organic materials in wastewater and microbial harboring in suspended solids content of effluent، its application in wastewater effluent is more costly than other usual disinfectants. Manuscript profile -
Open Access Article
14 - Relationship between Laboratory Exposure to Crude Oil and Anti-oxidative Responses of Tetraclita Rufotincta Barnacles
Mojgan Emtyazjoo Lida Salimi Majid Zeinali Bahar SHahabi Maryam Ghasempour malekiIntroduction: Nowadays the destructive effects of oil pollutions that produced by different resourcesin waters and aquatic organisms have been defined with the intention of studying effects of crude oilin different concentration, on variations of anti-oxidant enzymes le MoreIntroduction: Nowadays the destructive effects of oil pollutions that produced by different resourcesin waters and aquatic organisms have been defined with the intention of studying effects of crude oilin different concentration, on variations of anti-oxidant enzymes level (SOD, CAT)in Tetraclitarufotincta and the possibility of introduce these enzymes as biomarkers.Metod: Barnacles Tetraclita rufotincta were sampled in Bahrekan region. Barnacles exposed to250ppb, 125ppb, 62.5ppb, 31.25ppb, 15.625ppb and 3ppb as a control, crude oil after 24,48,72 and 96h. 15 barnacles removed from aquarium in average. Animals were examined for levels of Antioxidantsenzymes (SOD, CAT) in their tissues.Results: this study detected that in aquarium 1, with 3ppb concentration, after 24,48,72 and 96 hSuperoxide dismutase(SOD) activities shown respectively 128/98, 30/04, 75/8, 62/05U/ml/mg protein.In aquarium 2 and 3, with 15.625ppb and 31.25ppb concentration, after the period mentioned,superoxide dismutase(SOD) activities shown respectively 104/45, 74/95, 13/57, 109U/ml/mg proteinand 69/96, 61/56, 60/5, 86/46 U/ml/mg protein. In aquarium 4 and 5, with 62.5ppb and 125ppbconcentration, after the mentioned period, Superoxide dismutase(SOD) activities shown respectively98/79, 193/9, 42/75, 124/77 U/ml/mg protein and 69/22, 40/08, 81/86, 59/95U/ml/mg protein and inaquarium 6, with 250ppb concentration, after the mentioned period, Superoxide dismutase(SOD)activities shown respectively 62/11, 87/1, 20/71, 93/47U/ml/mg protein.The results also detected that in aquarium 1, with 3ppb concentration, after 24,48,72 and 96 h Catalase(CAT) activities shown respectively 13/29, 15/31, 15/5, 16/25 U/ml/mg protein. In aquarium 2 and 3,with 15.625ppb and 31.25ppb concentration, after the mentioned period, Catalase (CAT) activitiesshown respectively 13/03, 16/74, 13/65, 13/61U/ml/mg protein and 11/46, 16/54, 15/7, 13/58U/ml/mgprotein. In aquarium 4 and 5, with 62.5ppb and 125ppb concentration, after the mentioned period,Catalase (CAT) activities shown respectively 18/74, 11/86, 11/91, 16/22U/ml/mg protein and 21/1,1- PhD, Asst. Prof., Biology Dept, Islamic Azad University, Iran2- PhD, Instructor, Environment Pollution, Islamic Azad University, Iran3- PhD, Researcher, Biochemistry Dept, Research Institute of Petroleum Industry, Iran4- Department of Iranian offshore Oil company5- M. Sc, Marin Biology Dept, Islamic Azad University, IranAbstracts of Articles in EnglishJ.Env. Sci. Tech., Vol 17, No.1, Spring 201520415/8, 15/04, 39/22U/ml/mg protein and in aquarium 6, with 250ppb concentration, after the mentionedperiod, Catalase (CAT) activities shown respectively 15/54, 18/8, 15/81, 15/97U/ml/mg protein.Conclusion: There was no correlation between Superoxide dismutase (SOD) activities and differentconcentrations of crude oil, during the animals exposed to crude oil. It also detected that CAT enzymeis sensitive parameter than SOD and that could be useful biomarker for the evaluation of contaminatedaquatic ecosystems in Tetraclita rufotincta barnacles. Manuscript profile -
Open Access Article
15 - Investigation of Fenton Reagent Efficiency in Removal of Cetyl Trimethyl Ammonium Bromide from Synthetic Solution
Mohammad Reza Samarghandi Mansor ZarrbiIndustrial and municipal wastewaters are the main sources which contaminate the surface water and groundwater. Application of detergent in industrial and domestic uses increases this substance in wastewater. Detergents are large organic molecules that are slightly MoreIndustrial and municipal wastewaters are the main sources which contaminate the surface water and groundwater. Application of detergent in industrial and domestic uses increases this substance in wastewater. Detergents are large organic molecules that are slightly soluble in water, highly toxic, responsible for foaming in wastewater treatment plants and making interference with wastewater treatment operations. A numorous methods yet have been applied for the removal of detergent. These methods include coagulation and flocculation, reverse osmosis, oxidation by ozone and advanced oxidation processes. This study investigates the removal of cetyl trimethyl ammonium bromide from synthetic solution by Fenton reagent as an easy and effective method. Experiments were conducted in several stages with 100 cc of synthetic sample and pH=4, 5, 10, 15 and 20 mg/L of ferrous ions, 20, 30, 40 and 50 ml/L of hydrogen perocide in a batch reactor with contact time of 20, 40 and 60 min. Results showe that, in a batch system with acidic pH (pH=4), the removal of detergent increases with the increases of contact time, ferrous ions concentration and hydrogen peroxide. Therefore, in pH= 4, contact times of 60 min, and 20 mg/L of ferrous ions, Lthe removal efficiency increased from 54/5% to 89/5% by increase of hydrogen peroxide conventration from 20 ml/L to 50 ml/L. Finally, considering the simplicity and effectiveness of this method, it is be suggested to be used for the removal of detergent from municipal and industrial wastewaters. Manuscript profile -
Open Access Article
16 - Epoxidation of Alkenes and Oxidation of Alcohols with Hydrogen Peroxide Catalyzed by a Fe (Br8TPPS) Supported on Amberlite IRA-400
Shahla Masoudian Parvaneh Pakravan -
Open Access Article
17 - Antioxidant properties of orange and lemon peels and their efficacy in preventing lipid peroxidation in stored oils
Abdol Hadi Tajer Aisha Siddiqi Divya Prakash J. Jamuna Prakash Prabhavathi S.N. -
Open Access Article
18 - Spectrophotometric quantification of hydrogen peroxide utilizing silver nanozyme
Saeed Reza Hormozi Jangi Zahra DehghaniIn this contribution, an analytical method was developed utilizing silver nanozymes as inexpensive and high throughput nanomaterials with high peroxidase-like activity for the selective quantification of hydrogen peroxide in food samples. 3,3’,5,5’-tetrameth MoreIn this contribution, an analytical method was developed utilizing silver nanozymes as inexpensive and high throughput nanomaterials with high peroxidase-like activity for the selective quantification of hydrogen peroxide in food samples. 3,3’,5,5’-tetramethylbenzidine (TMB) was utilized as peroxidase substrate to produce the blue-colored analytical probe. The method was constructed based on the spectrophotometric probing the oxidation product of TMB by hydrogen peroxide in the presence of silver nanozyme as catalyst and the absorbance of the blue-colored oxidation product at 658 nm was used as analytical signal. The effective parameters on the method sensitivity including nanozyme amount, incubation time, buffer type and concentration, pH, and substrate concentration were optimized. In optimal experimental conditions, a linear dynamic range of 1-80 μM and detection limit as low as 0.12 μM were obtained. Besides, the selectivity studies revealed that the absorbance at 658 nm was increased in the presence of hydrogen peroxide while other co-existing species cannot proceed the oxidation process. Finally, the designed method was applied to quantify hydrogen peroxide of milk samples, revealing highly accurate results. The results of this research can be potentially utilized for food safety verification in food industries. Manuscript profile -
Open Access Article
19 - Evaluation Effect of Copper Sulphate and Manganese on Yield and Chemical Markers of Maize under Water Deficit Condition
Mohamad Reza Dadnia Reza Mori Sharyani -
Open Access Article
20 - The effect of zinc-methionine supplementation on antioxidant status and expression of interleukin-4 and interleukin-6 genes in female rats under heat stress
Matin Jamei Ali Asghar Sadeghi Mohammad ChamaniIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc suppl MoreIntroduction:During heat stress, the animal body requires more antioxidant compounds and activity of antioxidant enzymes. Zinc plays a role in the structure and activity of antioxidant enzymes. One of the ways to supply the zinc requirement, is to use organic zinc supplements, which zinc combined with methionine, and have more intestinal absorption Aim: This study was done to evaluate the effects of different doses of zinc-methionine supplementation on the blood antioxidant status and the expression of interleukin 4 and 6 genes in rats exposed to heat stress.Materials and methods: In a completely random design, 20 female rats were divided into four treatment groups with five replicates. Rats were kept at a temperature of 31±2 °C for 20 hours and at a temperature of 38±2 °C for 4 hours per day (to create heat stress). The rats in the control group were fed standard pellets without additives, and the three experimental groups were fed standard pellets plus 15, 30 and 45 mg zinc-methionine supplement per kilogram of dry matter for 30 days. At the end of the experiment, a blood sample was collected from the inferior vena cava. Total antioxidant capacity, malondialdehyde concentration, antioxidant enzymes, liver enzymes in the serum and the expression of interleukin-4 and interleukin-6 genes were measured.Results: Serum zinc concentration increased linearly with increasing zinc dose in the diet (P<0.05). The serum activity of two enzymes, alanine aminotransferase and aspartate aminotransferase decreased linearly with increasing zinc-methionine dose in the diet (P<0.01). The highest activity of glutathione reductase and glutathione peroxidase was observed in the group receiving 30 mg/kg, and the lowest activity of these enzymes was observed in the control group. In general, the relative expression of the interleukin-4 gene was increased, and the relative expression of the interleukin-6 gene was decreased (P<0.05). The highest expression of interleukin-4 gene belonged to the group receiving 30 mg, and the highest relative expression of interleukin-6 gene belonged to the control group and the group receiving 15 mg/kg.Conclusion: The results of this study showed that the dose of up to 30 mg of zinc-methionine per kg of diet used in this study increases the activity of superoxide dismutase enzyme and glutathione peroxidase enzyme activity, decreases the activity of liver enzymes in the serum, increases the expression of the anti-inflammatory gene (Interleukin-4) and decreases the expression of inflammatory gene (Interleukin-6), which indicates the reduction of oxidative stress and the reduction of the effects of heat stress in the body of the rat. Manuscript profile -
Open Access Article
21 - Investigating the effect of calcium on growth, oxidative indices, ascorbate, glutathione and antioxidant enzymes activity in triticale (× Triticosecale Wittmack) under salt stress
Malihe Jahani Mohammad Reza Hadi Mojtaba Jafarinia Sedighe JahaniIntroduction: Salinity is a developing problem in agricultural soils. Calcium plays an important role in the resistance of plants to salt stress. Aim: In order to investigate the mutual effect of sodium-calcium on growth, oxidative indices and antioxidant defense system MoreIntroduction: Salinity is a developing problem in agricultural soils. Calcium plays an important role in the resistance of plants to salt stress. Aim: In order to investigate the mutual effect of sodium-calcium on growth, oxidative indices and antioxidant defense system in triticale plant, an experiment was conducted as a completely randomized design with 3 replications in greenhouse conditions. Materials and methods: One week after planting seeds in the soil, seedlings were treated with sodium chloride dosages (0, 50, 100 and 150 mmol L-1) and calcium chloride dosages (0, 6 and 10 mmol L-1). After 5 weeks of stress, some morpho-physiological and biochemical parameters including shoot and root length, chlorophyll-meter number (SPAD), oxidative indices (malondialdehyde, other aldehydes, hydrogen peroxide and lipoxygenase enzyme activity), reduced ascorbate, dehydroascorbate, reduced glutathione and antioxidant enzymes activity (guaiacol peroxidase, catalase, superoxide dismutase, ascorbate peroxidase and polyphenol oxidase) of leaves was measured. Results: The results showed that salinity stress significantly decreased the length of shoot and root, SPAD, reduced ascorbate and reduced glutathione in leaf but increased the amount of oxidative indices, dehydroascorbate and antioxidant enzymes activity in leaf. While addition of calcium to the saline medium increased the length of shoot and root, SPAD, reduced ascorbate and reduced glutathione in leaf, but decreased the amount of oxidative indices, dehydroascorbate and antioxidant enzymes activity in leaf. Conclusion: Adding calcium to the saline medium reduced the harmful impacts of salinity stress and the most beneficial impacts of calcium were observed at a concentration of 6 mmol/ L-1. Manuscript profile -
Open Access Article
22 - Wheat Response (Triticum aestivum L.) to Selenium under Normal Irrigation and Water Deficit Conditions
Mohammad Reza DadniaTo evaluate the effect of selenium (from sodium selenate) on increasing efficiency at water deficit condition a research was conducted in Karaj 1 wheat cultivar in 2014-2015 cropping year with split plot based on completely randomize block design with four replications MoreTo evaluate the effect of selenium (from sodium selenate) on increasing efficiency at water deficit condition a research was conducted in Karaj 1 wheat cultivar in 2014-2015 cropping year with split plot based on completely randomize block design with four replications in Karaj. Irrigation treatments with two levels, normal (I1) and interruption of irrigation at mid flowering (110 days after planting) (I2), which were assigned to main plots and selenium foliar application with six concentrations: 0 (S0), 5 (3.12 micro g.) (S1), 10 (6.24 micro g.) (S2), 15 (9.36 micro g.) (S3), 20 (12.5 micro g.) (S4) and 25 (15.62 micro g,) of sodium selenate per plant (S5) g.ha-1 to sub plots. The results showed that water deficit increased the rate of superoxide dismutase, catalase, glutathione peroxidase and malondialdehyde marker. Foliar application of selenium, increased contents of antioxidant (anti oxidation) enzymes under stress condition and was maximum in 20 g. selenium per ha-1. Content of malondialdehyde was decreased and caused significant increase in grain yield. Increasing antioxidants and reduction of malondialdehyde at normal condition was lower. On the other hand, negative effect of malondialdehyde on yield components, cellular membrane stability and antioxidant enzymes at humidity stress condition was higher than normal condition. Significant increase of cytoplasmic membrane stability, grain yield, grain weight and selenium content of seed was obtained when 15 and 20 g. selenium ha-1 were used as compared with those of higher and lower rates of selenium usage under water deficit and normal irrigation. Suppression of free oxygen radicals, and enzyme induction defense mechanisms against oxidative damage has been effective. Therefore, use of exogenous compounds such as selenium may increase the antioxidant capacity of plant against stress. Manuscript profile -
Open Access Article
23 - Morpho- Physiological Changes of Hempseed (Cannabis sativa L.) Traits as Affected by Seed Priming with Folic Acid and Hydrogen Peroxide
Shirin Karbalaye Golizadeh Touraj Mir Mahmoodi Nabi Khalili AqdamTo evaluate the effects of seed priming of hempseed with folic acid and hydrogen peroxide on some morphological and physiological traits a factorial greenhouse experiment based on randomized complete design with four replications was conducted at Islamic Azad University MoreTo evaluate the effects of seed priming of hempseed with folic acid and hydrogen peroxide on some morphological and physiological traits a factorial greenhouse experiment based on randomized complete design with four replications was conducted at Islamic Azad University, Mahabad Branch. Treatments consisted of hydrogen peroxide at five levels (0, 7.5, 15, 22.5, 30 mm/liter) as the first factor and the four leveld of folic acid (5, 10, 20, 27 mm/liter) as the second factor.Seeds, to be primed, were immersed into solution of folic acid for 24 hours and hydrogen peroxide for 6 hours. The characteristics like chlorophyll a, chlorophyll b, total chlorophyll content, relative water content, plant height, root length, allometric coefficient, plant fresh and dry weights, were measured. Result of analysis of variance showed that the effects of folic acid and hydrogen peroxide on all characters were significant, but the interaction between the two treatments were only significant on relative water content and allometric coefficient. In this study, seed priming with 15 mm/liter of hydrogen peroxide and 5mm folic acid resulted in highest chlorophyll a, chlorophyll b, total chlorophyll contents, plant height, root length, fresh and dry weights. Increasing hydrogen peroxide level above 15mm/liter affected traits negatively. Combinated treatments of 15 mm/liter of hydrogen peroxide and 5 and 10 mm of folic acid resulted in highest relative water content and allometric coefficient, respectively. Based on the results obtained it can be concluded that priming seeds with 15 mm/liter of hydrogen peroxide and 5mm folic acid is recommended to produce proper morphological and physiological traits. Manuscript profile -
Open Access Article
24 - Effect of Humic Acid on Activity of Antioxidant Enzymes and Yield of Castor Bean (Ricinus commonis) under Water Deficit Condition
Mohammad Reza DadniaTo evaluate the effect of humic acid on activity of antioxidant enzymes in castor bean a split plot experiment based on completely randomize block design with four replications was carried out at Islamic Azad University, Karaj Branch in 2014. Treatments were irrigation MoreTo evaluate the effect of humic acid on activity of antioxidant enzymes in castor bean a split plot experiment based on completely randomize block design with four replications was carried out at Islamic Azad University, Karaj Branch in 2014. Treatments were irrigation with three levels [irrigation at 80% of field capacity (S1) (normal), irrigation at 65% of field capacity (S2) (mid stress) and irrigation at 50% of field capacity (S3) (high stress)] assigned to main plots and humic acid with four levels (without foliar application, one time, twice and three times foliar application) to sub plots. In this study traits like seed yield, 100 seed weight and antioxidant enzymes were estimated. The results showed that the effect of humic acid was significant at 1% level on 100 seed weight and activity of antioxidant enzymes. Mean comparisons indicate that foliar application of humic acid could compensate relative effect of water deficit. It was also revealed that the rate of superoxide dismutase, glutathione peroxidase and catalase were increased with three times of humic acid foliar applications by 38.8, 34.75 and 37.88 percent under mid stress drought and 26.63, 41.15 and 43.14 percents under high stress drought as compared to control, respectively. Overall, it can be said that use of humic acid have different physiological effects. Humic acid not only increases seed yield of castor bean it also have a positive role in reducing water deficit effects and increasing activity of antioxidant enzymes. Manuscript profile -
Open Access Article
25 - The Effect of Biochar and Humic Acid Rates on some Phophysiological Characteristics and Grain Yield SC704 Corn (Zea mays L.) Hybrid Under Water Deficit stress
Armaghan Charkhab Many Mojaddam Shahram Lack Tayyeb Sakinejad Mohammad Reza DadniaTo evaluate the effect of different rates of biochar and humic acid applications on physiological characteristics and grain yield of single cross 704 hybrid corn under water deficit stress, a split split plot experiment based on a randomized complete block design with t MoreTo evaluate the effect of different rates of biochar and humic acid applications on physiological characteristics and grain yield of single cross 704 hybrid corn under water deficit stress, a split split plot experiment based on a randomized complete block design with three replications, was conducted during two years (2017-19) at Ahvaz. The main plot devoted to water deficit stress, with three levels irrigation (after depleting 30, 40, and 50% of field capacity as optimum irrigation, moderate stress, and severe stress, respectively) and sub-plot to biochar with 2 levels, non-application of biochar (control) and application of 4 tons per hectare of biochar, and the sub-sub plots to humic acid with 4 levels non-application of humic acid (control), and application of 2, 4, and 6 liters per hectare of humic acid. The results showed that the interaction effect of water shortage by biochar stress and water shortage by humic acid stress on grain yield, 1000-grain weight, number of grains per ear, superoxide dismutase, chlorophyll index and catalase were significant at 1% probability level. Under severe stress conditions grain yield (36.54%), number of grain per ear (36%) and leaf area index (30.36%) decreased and activity of superoxide dismutase (37.7%) increased. Acidic acid had a significant effect on all traits under study. The highest grain yield (8995.18 kg.ha-1) was related to irrigation treatment after 40% depletion of field capacity and application of 4 tons per hectare of biochar. Based the results obtained application of 4 liters per hectare of humic acid under moderate moisture stress conditions, could be recommended in arid and semi-arid conditions to save water consumption and reduce the effects of water deficit stress. Manuscript profile -
Open Access Article
26 - Application of ozone for reducing the microbial count of mechanically deboned chicken meat
M. H. Eskandari S. hasheminasab M. niakosari S. ShekarforoushOzone is a strong oxidant and potent disinfecting agent and it has gained a lot of applications to preserve food commodities. The aim of this study was to investigate the effect of ozonated water on the shelf-life of mechanically deboned chicken (MDC) during storage at MoreOzone is a strong oxidant and potent disinfecting agent and it has gained a lot of applications to preserve food commodities. The aim of this study was to investigate the effect of ozonated water on the shelf-life of mechanically deboned chicken (MDC) during storage at 4 °C. Five different MDC samples were treated with ozone (0, 0.032, 0.065, 0.098, 0.13 ppm/g) and individually wrapped and stored at 4 ± 2 °C. The samples were analyzed for chemical (peroxide value) and microbiological (aerobic mesophilic counts, psychrotrophic counts, Staphylococcus aureus counts, coliform counts and mold and yeast counts) at 0, 3, 6, 9, 12 and 15 days of refrigerated storage. The result showed that in comparison with the control sample, ozone treatment caused a significant (P Manuscript profile -
Open Access Article
27 - The Reaction of Some Qualitative Changes in the kernel of Different Walnut Genotypes in the Storage Period
Zahra Davarkhah Mehdi Hosseinifarahi محسن Radi Sedigheh GholipourThe walnut has a multipurpose use, so that it is cultivated in pomology for its fruit, in forestry for the use of wood, in pharmaceuticals as a medicinal plant, and in parks as an ornamental plant. In order to investigate some qualitative postharvest characteristics th MoreThe walnut has a multipurpose use, so that it is cultivated in pomology for its fruit, in forestry for the use of wood, in pharmaceuticals as a medicinal plant, and in parks as an ornamental plant. In order to investigate some qualitative postharvest characteristics the kernel of 14 superior walnut genotypes (Sisakht 1 and 2, Delirej 1 and 2, Shahniz 1 and 2, Kowkhdan 1 and 2, Setangan 1 and 2, Ganjegun 1 and 2 and Vezeg 1 and 2), an experiment was conducted in Kohgiluyeh and Boyar Ahmad provinces during 2019 to 2020. Qualitative characteristics such as oxidative stability of walnut kernels (peroxide value), percentage of moisture and weight loss of kernels after 6 months of storage and sensory evaluation after 14 months of storage at 25°C were evaluated. The results showed that the peroxides value in walnut kernels increased during storage. After 6 months of storage in the warehouse, the lowest peroxide value was observed in the genotypes of Vezg 1 and Ganjegun 2. The results of the sensory evaluation showed that Ganjegun 2 and Vezg 1 genotypes had the highest and Delirej 1 and 2 genotypes showed the lowest overall acceptance rate after 14 months of storage. Finally, Vezg 1 and Ganjegun 2 genotypes are recommended for consumption and also in breeding programs due to their better quality and durability after harvesting. Manuscript profile -
Open Access Article
28 - Antioxidant effect of pistachio peel extract and aerobic exercise on superoxide dismutase, catalase and malondialdehyde in the latissimus dorsi muscle of overweight Wistar rats
Mohsen Sahebi Mohamad ali Azarbayjani Maqsood PiriAim: The objective of this study was to detect the antioxidant effect of pistachio green hull extract and aerobic exercise on superoxide dismutase, catalase, and malondialdehyde in the external vastus muscle of overweight Wistar rats.Method: This study is in the categor MoreAim: The objective of this study was to detect the antioxidant effect of pistachio green hull extract and aerobic exercise on superoxide dismutase, catalase, and malondialdehyde in the external vastus muscle of overweight Wistar rats.Method: This study is in the category of interventional studies and is included in the category of experimental studies. In this study, 30 Wistar (female) rats with an age range of 12-13 weeks and a weight range of 180 to 200 grams were present. The samples are in 5 groups including: 1- control group (Con), obese control group (OB-Con), obese group and aerobic exercise (OB-AT), obese group and pistachio skin extract (OB-PGH), obese group and Pistachio green hull extract and aerobic exercise (OB-PGH-AT) were divided.Findings: Exercise had a significant effect on muscle dismutase superoxide concentration (F=10.61, P=0.004, ƞ=0.347). But receiving pistachio skin extract had no significant effect on muscle dismutase suboxide concentration (F=2.42, P=0.135, ƞ =0.108). The interaction between exercise and pistachio skin extract had no significant effect on muscle dismutase suboxide concentration (F=0.75, P=0.396, ƞ=0.036). Exercise had a significant effect on catalase concentration (F=160.98, P=0.001, ƞ=0.889). Taking pistachio skin extract also had a significant effect on muscle catalase concentration (F=5.04, P=0.036, ƞ=0.201). But the interaction between exercise and pistachio skin extract had no significant effect on muscle catalase concentration (F=1.84, P=0.190, ƞ=0.084). Exercise had a significant effect on muscle malondialdehyde concentration (F=57.66, P=0.001, ƞ=0.742). Taking pistachio skin extract also had a significant effect on muscle malondialdehyde concentration (F=80.29, P=0.001, ƞ=0.801). However, the interaction between exercise and pistachio skin extract had no significant effect on muscle malondialdehyde concentration (F=0.004, P=0.949, ƞ=0.001).Conclusion: In addition, adding PGH supplements to aerobic exercise can reduce oxidative damage and increase the antioxidant enzymes catalase and superoxide dismutase. Induction of obesity increases inflammatory markers and oxidative stress in muscle. Manuscript profile -
Open Access Article
29 - Investigating the Effect of Plant Growth Regulators (GA3 and 2, 4-D) on the Reduction of the Physiological Disease of Thomson-Novel Orange Fruit
shahruz habibi ali ebadi alireza ladan moghadam siavash raytpanahCitrus is a major product of the gardens of the north of the country, and since oranges have better storage, they also have a larger share of the gardens. Oranges have a very good market and have a good market and have the highest production, but because it has thin nav MoreCitrus is a major product of the gardens of the north of the country, and since oranges have better storage, they also have a larger share of the gardens. Oranges have a very good market and have a good market and have the highest production, but because it has thin navel and skin, it is sensitive to tension and has a physiological disorder Fruiting. In order to reduce this complication, a randomized complete block design with 2 and 4 dichlorophenoxy acetic acid (2, 4-D) at concentrations of 0-10 and 20 mg / L and gibberellic acid GA3 (with concentrations of 0- 50 and 100 mg / L at flowering time, petals fell immediately after the physiological fall of fruit for two consecutive years (2016-2017). In this experiment, the percentage of fruit grazing, fruit yield, fruit skin thickness, soluble solids, cellulase and superoxide dismutase enzyme activity were measured. Based on the results obtained from the comparison of the meanings, the maximum thickness of the fruit skin, soluble solids, performance and activity level of the enzyme superoxide dismutase, as well as the lowest activity of the enzyme cellulase Manuscript profile -
Open Access Article
30 - Changes of antioxidative systems in various color leaves of Japanese spindle (Euonymus japonicus Thunb.)
Nader Chaparzadeh Samane Safikhani Laila Zarandi-MiandoabSome physiological parameters and antioxidant responses were investigated in different color leaves of the evergreen shrub Japanese spindle (Euonymus japonica Thunb.). The shrub has leaves with three colors including dark green, light green, and yellow. Oxidative stress MoreSome physiological parameters and antioxidant responses were investigated in different color leaves of the evergreen shrub Japanese spindle (Euonymus japonica Thunb.). The shrub has leaves with three colors including dark green, light green, and yellow. Oxidative stress markers (hydrogen peroxide concentration, cell membrane stability, and lipids peroxidation), activity of peroxidase and some antioxidant molecules (phenolic compounds, proline, and free amino acids) were studied. Significant differences were found in hydrogen peroxide content, cell membrane stability, and membrane lipids peroxidation among leaves. In yellow leaves, the total content of proline and free amino acids were higher than those of the dark green and light green leaves. The highest and lowest free phenolic compounds contents were found in the dark green and yellow leaves, respectively. The POD activity increased significantly with changing leaf color from dark green to light green and to yellow. As a result, despite the activity of the antioxidant systems, the color change from dark green to yellow caused a gradual increase in the oxidative damage. Manuscript profile -
Open Access Article
31 - Effects of methanol spraying on some biochemical and physiological characteristics of soybean (Glycine max L.) under drought stress
Behzad Amraei Farzad Paknejad Mohammadali Ebrahimi Hamid SobhanianIn order to investigate the effect of foliar application of methanol and drought stress on some biochemical properties of soybean (Glaycine max L.), a pot experiment was done in farm environment. The experiments were done in factorial form based on a completely random d MoreIn order to investigate the effect of foliar application of methanol and drought stress on some biochemical properties of soybean (Glaycine max L.), a pot experiment was done in farm environment. The experiments were done in factorial form based on a completely random design with 3 repetitions. Treatments included three irrigation levels of normal (irrigation after 40% depletion of available soil moisture), average stress (irrigation after 60% depletion of available soil moisture), and severe stress (irrigation after 70% depletion of available soil moisture) as the main factor and levels of methanol in the form of foliar application including control solutions (foliar application without use of methanol) and solutions of 14.7% and 21% of methanol as secondary factor. Results obtained from the study showed that there were significant differences between various levels of methanol in content of chlorophyll and carotenoid compounds, relative water content, phenolic compounds, total protein content, proline, and leaf peroxide hydrogen in (P≤0.01). With the application of stress from mild to severe, application of 14% methanol showed more pronounced effects on total chlorophyll content, chlorophyll a and chlorophyll b. Moreover, under mild and severe stress conditions, with application of the highest value of methanol, production of peroxide hydrogen reached lowest level and the content of phenolic compounds increased with the increased application of methanol from 7% to 14%. According to the obtained results, with application of 14% methanol, more increase was observed in the efficiency of proline under severe stress conditions. Increasing the volume of methanol from 7 to 14%, the relative water content was preserved under stress conditions. For protein, with increased drought stress, the effect of application of 14% methanol was the same in comparison with 21% methanol. Therefore, according to the obtained results, it is concluded that methanol could improve plant resistance against drought stress. Manuscript profile -
Open Access Article
32 - The comparison of oil biochemical compositions of three olive cultivars (Olea europaea L.) in different regions of Golestan province
Esmaeil Seifi Arezoo Jalali Somayyeh Ebraheimnia Hossein FereidooniOlive has a suitable oil, which shows excellent nutritional values. Due to our country's huge need to edible oil and the high quality of olive oil, developing the area under cultivation of olive is an important aim of Iran's agricultural programs. In this study, three c MoreOlive has a suitable oil, which shows excellent nutritional values. Due to our country's huge need to edible oil and the high quality of olive oil, developing the area under cultivation of olive is an important aim of Iran's agricultural programs. In this study, three cultivars including Mission, Koroneiki and Zard from three regions in Golestan province (Varsan, Ganareh and Minoodasht) were studied in terms of quantity and quality of the oil. Results showed that there were some significant differences among various regions and cultivars. Mission in Varsan and Koroneiki in Minoodasht had the highest and lowest fruit weight, respectively. Cultivars Mission and Zard in Ganareh and Mission in Varsan had the highest percentage of dry matter in fruits. The highest percentages of oil in dry and fresh weights were observed in cultivars Koroneiki and Zard in Ganareh, respectively, though they were not significantly different from the other treatments. In term of oil physicochemical traits, cultivar Mission in Ganareh showed the lowest free fatty acids. Cultivar Koroneiki Minoodasht had the lowest K232 value; while, it had the highest amount of total chlorophyll and carotenoid. The oil produced in Varsan region had the lowest and highest amount of peroxide and K270 value, respectively. In general, the results showed that cultivar Koroneiki is suitable to produce in all three regions of Varsan, Ganareh and Minoodasht. Manuscript profile -
Open Access Article
33 - Effect of nano-TiO2 and salicylic acid foliar application on some physiological traits of Echinaceae purpurea L. under water deficit stress
Asiyeh Omid-Haghi Ebrahim Khalilvand Behrouzyar Farhad Farahvash Mehrdad Yarnia Abdollah HasanzadehIn order to investigate the effect of drought stress and nano-TiO2 and salicylic acid foliar application on some physiological traits of Echinaceae purpurea under water, an experiment was conducted in split plot factorial based on RCBD in three replications at the Resea MoreIn order to investigate the effect of drought stress and nano-TiO2 and salicylic acid foliar application on some physiological traits of Echinaceae purpurea under water, an experiment was conducted in split plot factorial based on RCBD in three replications at the Research Station of the Islamic Azad University, Tabriz Branch, during growing seasons of 2017-2018. The main factors were water deficit stress at three levels of 50, 75, and 100% filed capacity, thefactorial combination of nano-TiO2 (n-TiO2) foliar application at two levels (0.02 and 0.04%) and salicylic acid foliar application at two levels (0.02 and 0.04%), and also distilled water foliar application as control treatment. Findingsshowed a significant effect of water deficit stress on relative water content (RWC) and proline (p≤0.01). Also, interaction of the effects of water deficit stress and nano-TiO2 and salicylic acid foliar application on Chl. b, total Chl., and hydrogen peroxide contents was significant (p≤0.01). Application of water deficit stress treatment up to 50% FC increased proline content by 40% and reduced RWC by 10% compared to complete irrigation (100% FC). Furthermore, 0.04% n-TiO2 and 0.04% SA foliar application increased hydrogen peroxide content by 50% compared to complete irrigation group and the control. The maximum total Chl. Contents of leaves was obtained under 100% FC irrigation and and 0.04% n-TiO2 and 0.02% SA foliar application showing 79.52% increase compared to 100% FC irrigation and no foliar application. Manuscript profile -
Open Access Article
34 - Study of protein pattern, antioxidant enzymes and accumulation of cadmium in sunflower (Helianthus annuus L.) treated with cadmium and salicylic acid
Sakineh MoradkhaniIn order to investigate the interaction effects of cadmium as a heavy metal and salicylic acid as an antioxidant and anti-stress substance on sunflower plants, sunflower seeds were washed with distilled water after disinfection with 1% sodium hypochlorite solution and w MoreIn order to investigate the interaction effects of cadmium as a heavy metal and salicylic acid as an antioxidant and anti-stress substance on sunflower plants, sunflower seeds were washed with distilled water after disinfection with 1% sodium hypochlorite solution and were planted in pots containing sandy soil in a growth chamber with a temperature of 34 ℃ during the day and 25 ℃ at night, and 16/8 hours of light/dark photoperiod. This research was performed as a factorial completely randomized design with four replications under greenhouse conditions. Sunflower plants were exposed to cadmium chloride treatment (0, 50, 100, 150 and 200 µM) at two-leaf stage on a weekly basis. One week after cadmium treatment, plant leaves were sprayed with salicylic acid (0, 250 and 500 µM). After harvesting the plants, cadmium concentrations in roots and shoots were measured. Then, antioxidant enzymes were assayed. Total protein content of leaf samples was extracted and the pattern of leaf protein changes was investigated by SDS-PAGE electrophoresis. Cadmium concentration in roots and shoots increased with increasing cadmium and salicylic acid treatment. Catalase enzyme activity decreased with increasing cadmium concentration, and salicylic acid had a very small effect on its activity. The activity of superoxide dismutase, ascorbate peroxidase, and glutathione reductase enzymes increased with increasing cadmium concentration while increasing salicylic acid concentration reduced the activity of these enzymes. Leaf protein electrophoresis under cadmium and salicylic acid treatments showed differences in leaf protein intensity compared with control plants. The expression of some proteins was increased or decreased in the treated plants. Therefore, cadmium and salicylic acid treatments made changes in the amount of cadmium uptake, antioxidant enzymes activity, and the pattern of sunflower leaf proteins. Manuscript profile -
Open Access Article
35 - Effect of salicylic acid application on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress
Mohammad Moghaddam Leila Mehdizade Sara FarsariThe aim of this research was to study the effect of salicylic acid (SA) usage on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress. For this purpose, a pot factorial experiment was performed base MoreThe aim of this research was to study the effect of salicylic acid (SA) usage on some physiological characteristics and essential oil production of lemon verbena (Lippia citrodora L.) under salinity stress. For this purpose, a pot factorial experiment was performed based on a completely randomized design with 4 salinity levels (0, 50, 100 and 150 mM NaCl), 4 SA levels (0, 150, 300 and 450 mg/L) and three replications. The measured traits included total soluble protein, malondialdehyde (MDA), hydrogen peroxide, glycine betaine, some antioxidant enzyme activities, and essential oil production. The interaction effect of the treatments on all of the studied traits were significant at 1% P value. The results showed that the highest amount of total soluble protein, MDA, hydrogen peroxide, glycine betaine, ascorbate peroxidase and superoxide dismutase activities were observed at 150 mM salinity level and application of SA at 300 mg/L causes to decrease total soluble protein and glycine betaine, and 150 mg/L of SA causes to decrease MDA. But application of this plant growth regulator had no significant effect on hydrogen peroxide. Also, the results showed that catalase enzyme activity and essential oil content at the highest salinity level (150 mM) reached to the lowest amounts. In total, the results of this study indicated that the SA application especially at 300 mg/L causes to improve the studied traits in this experiment and its usage under stress conditions is recommended. Manuscript profile -
Open Access Article
36 - Yield of different wheat (Triticum aestivum L.) cultivars under application of different fertilizer sources in dryland farming
Rahim Naseri Amir Mirzaei Abas SoleymanifardIn order to investigate the effect of growth-promoting bacteria on physiologic traitsof new dryland wheat cultivars, a factorial field experiment was carried out in a randomized complete block design with three replications on the farms of Sarablah Agricultural Research MoreIn order to investigate the effect of growth-promoting bacteria on physiologic traitsof new dryland wheat cultivars, a factorial field experiment was carried out in a randomized complete block design with three replications on the farms of Sarablah Agricultural Research Center in the cropping season during 2019-2020. Experimental treatments included different wheat cultivars (Sardari, Karim, Koohdasht, and Rijaw) and different fertilizer sources including control (without fertilizer source), 50% N fertilizer, Azospirillum+50% N fertilizer, Azetobacter + 50% N fertilizer, Azospirillum + Azetobacter+50% N fertilizer, and 100% N fertilizer. The results of this experiment showed that the interaction effect of cultivar × fertilizer sources had a significant effect on the activity of some antioxidant enzymes and physiological properties. Rijaw cultivar × Azospirillum + Azetobacter+ 50% N fertilizer increased the activities of ascorbate peroxidase (80.9%), glutathione peroxidase (80%), catalase (71.7%), peroxidase (74.1%), superoxide dismutase (74%), carotenoids (72.1%), chlorophyll a (86.1%), chlorophyll b (88.2%), and relative water content (45.4%) while reduced the amount of Malondialdehyde (65.9%) and hydrogen peroxide (91.8%). Moreover, Sardari cultivar × control treatment (without fertilizer sources) had the lowest activities of ascorbate peroxidase, peroxidase, superoxide dismutase, and photosynthetic pigments. Therefore, according to the results of the study, wheat cultivar Rijaw × Azospirillum + Azetobacter+ 50% N fertilizer may be recommended for cultivation in dryland conditions considering the improved physiological characteristics and ultimately increased plant growth. Manuscript profile -
Open Access Article
37 - The study Effect of teratments salicylic acid and selenium on physiological and agronomic characteristics of safflower in drought stress conditions
saedeh rashidyIn order to investigate the effect of salicylic acid and selenium foliar application on physiological characteristics and yield and yield components of safflower under drought stress conditions, an experiment was conducted as split plots based on randomized complete blo MoreIn order to investigate the effect of salicylic acid and selenium foliar application on physiological characteristics and yield and yield components of safflower under drought stress conditions, an experiment was conducted as split plots based on randomized complete blocks design with three replications. The main factor included irrigation in three levels, complete irrigation during growing season (control), cutting irrigation at 50 percent flowering stage and cutting irrigation at 50 percent head forming stage. Sub factor was considered foliar application of safflower plants including nonspraying (control), foliar application by salicylic acid, selenium and salicylic acid with selenium. Drought stress reduced biologic yield, yield components and grain yield, but it increased protein percentage, the superoxide dismutase activity and proline concentration. Also, the highest negative effect of drought tension was observed in flowering stage, so that stress at flowering stage reduced 49 percent grain yield and 33 percent biomass of safflower. Foliar application improved yield, yield components and superoxide dismutase activity compared to non-spraying (control). However, between foliar application treatments in grain yield and superoxide dismutase activity, no significant difference was observed, but foliar application of salicylic acid with 7198 kg ha-1 showed the highest biomass. The foliar application in stress and non-stress conditions increased grain oil percentage, so that in complete irrigation and flowering stage stress , the highest grain oil percentage was belonged to salicylic acid foliar application with 24 percent and salicylic acid foliar application with selenium 28 percent. The foliar application treatments increased proline concentration in stress conditions compared to control and at flowering stress stage, salicylic acid and selenium foliar application with 11 micro Mol per gram fresh plant weigh showed the highest proline concentration. Keywords: Grain yield, Oil percentage, Proline and Safflower. . Manuscript profile -
Open Access Article
38 - Evaluation of some root growth traits and activity of enzyme and nonenzyme antioxidants of different cultivars of durum wheat (Triticum turgidum var. durum) effected by phosphorous fertilizer and mycorrhizal fungi in rainfed condition
Houshang Naseri Rad Rahim Naseri In order to evaluate the effect of mycorrhizal fungi and phosphorous fertilizer on some root growth traits and activity of enzyme and nonenzyme antioxidants of durum wheat in rainfed conditions, an experiment was carried out as factorial based on a randomized comp More In order to evaluate the effect of mycorrhizal fungi and phosphorous fertilizer on some root growth traits and activity of enzyme and nonenzyme antioxidants of durum wheat in rainfed conditions, an experiment was carried out as factorial based on a randomized complete block design with three replications at Sarableh Agricultural Research station during growing season 2018-2019. Four cultivars of durum wheat (Dehdasht, Zahab, Savarz and Saji) as the first factor and five levels of fertilizer source (control, 25 and 50 kg.ha-1 P, mycorrhizal fungi (GM), mycorrhizal fungi + 25 kg.ha-1 P) as the second factor were considered. The average comparison results of simple effects indicated that Zahab and Saji cultivars among cultivars and combination treatment of mycorrhizal + 25 kg / ha P and then 50 kg / ha P had the greatest effect on improving the studied traits. The interaction effect of cultivars and fertilizer sources revealed that the combined use of phosphorous fertilizer and mycorrhizal fungi had better results compared to their use alone. So that, the highest fresh and dry weight, length density, and root water content and activity of ascorbate peroxidase, peroxidase, catalase, superoxide dismutase and glutathione synthetase was obtained in combined treatment of mycorrhizal and phosphorus fertilizer at Zahab and Saji cultivars. However, the lowest specific root length, and malondialdehyde and hydrogen peroxide activity were obtained in the same treatment and cultivars. In general, the results showed that inoculation with mycorrhizal fungi in rainfed conditions, especially in Zahab and Saji cultivars, in addition to reducing the application of phosphorus fertilizer can improve root growth characteristics and activity of enzyme and nonenzyme antioxidants. As a result, it reduces the peroxidation of membrane lipids (the decline in production of malondialdehyde and hydrogen peroxide) and increases drought stress tolerance. Manuscript profile -
Open Access Article
39 - Effects of cycocel and micronutrients on yield, physiological traits, and antioxidant activity of maize (Zea mayz L.) in low irrigation conditions at the end of the season
Seyed Nouradin Ghaffari Alireza Shokuhfar Mani Mojaddam Shahram Lack Aziz AfarineshIn order to investigate the effect of cycocel and micronutrient consumption on yield, physiological traits, and antioxidant content of maize under low irrigation conditions at the end of the season, a split factorial experiment was carried out based on a randomized comp MoreIn order to investigate the effect of cycocel and micronutrient consumption on yield, physiological traits, and antioxidant content of maize under low irrigation conditions at the end of the season, a split factorial experiment was carried out based on a randomized complete block design with four replications in Safiabad Agriculture Research Center in Dezful during two cropping years 2017-18 and 2018-19. The main treatment included water stress with three levels control (without water stress), water stress in female flower formation stage and water stress in grain swelling stage. Also, foliar application of cycocel at three levels of control (no use of cycocel), 442.5 ppm, and 885 ppm and three levels of micronutrient fertilizers, namely control (no fertilizer application), foliar application of 1000 g. ha-1, and foliar application of 1500 g. ha-1 were considered as the sub-factors. Results of the combined ANOVA showed that the interaction of the effects of year, stress, micronutrient, and cycocel on relative water content, proline and grain yield and also the interaction of the effects of stress, micronutrient, and cycocel on hydrogen peroxide, glutathione peroxidase, and soluble proteins were statistically significant (p≤0.01). The results also showed that the highest activity of superoxide dismutase enzyme was obtained under stress conditions at the grain swelling stage, which showed an increase by about 37% compared to the irrigation treatment under optimal conditions. Foliar application of 1500 g. ha-1 micronutrient and 885 ppm cycocel in non-stress conditions increased the average grain yield (11500 kg. ha-1) in the second year by about 85% compared to stressed condition at grain swelling stage and no application of micronutrient and cycocel. In general, to increase grain yield under optimal irrigation conditions and reduce the drop in stress conditions, growth regulators such as cycocel with a concentration of 885 ppm and micronutrient foliar application at 1500 g. ha-1 are recommended. Manuscript profile -
Open Access Article
40 - Response of genotypes of Foeniculum vulgare Mill. to salicylic acid priming under salinity stress
Sanaz Adalatzadeh-Aghdam Mahmoud Toorchi Mahmoud ZareiThe current study agreenhouse research was designed to evaluate the reaction of fennel (Foeniculum vulgare Mill.) genotypes to salt stress (NaCl salinity) and Salicylic acid, to study of the proteome profile of leaves for identifying molecular pathway involved in salt s MoreThe current study agreenhouse research was designed to evaluate the reaction of fennel (Foeniculum vulgare Mill.) genotypes to salt stress (NaCl salinity) and Salicylic acid, to study of the proteome profile of leaves for identifying molecular pathway involved in salt stress tolerance. The study was designed as factorial experiment using a randomized complete block design in three replications. Treatments are included NaCl solutions at three levels (0, 100, 200 mM) as salinity stress, Salicylic acid at two levels (0, 0/5 mM) and five genotypes. Agronomic and physiochemical traits were included: fresh and dry weight of ariel plant and roots, plant height, root length, fresh and dry weight of total plant, chlorophyll a, b and carotenoids, total chlorophyll, Na+ and K+ content, K+/Na+ ratio, malondialdehyde (MDA), Hydrogen peroxide (H2O2) and Total soluble carbohydrate (TSC) of leaves. The analysis of variance revealed significant differences for main and interaction effects in examined traits. German and Moghan genotypes were expressed as the most tolerant and sensitive genotypes based on Arunachalam method, respectively. Manuscript profile -
Open Access Article
41 - Study of Differential Responses of Anti Oxidative Enzymes of Two Sensitive and Tolerant Cultivars of Wheat (Triticum aestivum L.) to Salt Stress
فائزه Ghanati, الهام Nayyeri Torshizi,In this article the effects of salt stress on the activity of antioxidative anzymes and lipid peroxidation were studied in shoots and roots of two cultivars of wheat. The cultivar Mahooti and Alamoot were selected as salt-tolerant and salt-sensitive, respectively. The p MoreIn this article the effects of salt stress on the activity of antioxidative anzymes and lipid peroxidation were studied in shoots and roots of two cultivars of wheat. The cultivar Mahooti and Alamoot were selected as salt-tolerant and salt-sensitive, respectively. The plants were treated for 24, 48, 96 hours. Mostly salt stress increased the activity of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in treated plants. Also, the increase in level of CAT activity in roots was more pronounced in the salt-tolerant than in the salt-stress that is indicated the role of CAT for tolerance in Mahooti. The increase in activity of these enzymes during 24 hours of treatment with NaCl in salt-tolerant plants were more than in salt-sensitive plants. Lipid peroxidation had a little increase in treated plants, compared to those of the control ones. Salt stress had no significant effect on accumulation of Na+ in two cultivars. The results indicated that the oxidative stress play an important role in the activity of different enzymes in salt-stress and salt-tolerant plants. Also, rate of enzyme activities showed some flactuations, which suggest the cooperation of antioxidant enzymes with each other corresponding to different times and different stages of the growth. Manuscript profile -
Open Access Article
42 - The effect of water stress on various of growth and proline, catalase, SOD in sesbania aculeata
مظفر Kamrani آرین Sateei عباسعلی NouriniaIn this study, effects of dry stress on growth parameters, proline content, glycine betaine, catalase, proxidase, and superoxide dismutase (SOD) were investigated in Sesbania aculeate L. This study was carried out fewer than 7 potting conditions on 7 treatments. Growth MoreIn this study, effects of dry stress on growth parameters, proline content, glycine betaine, catalase, proxidase, and superoxide dismutase (SOD) were investigated in Sesbania aculeate L. This study was carried out fewer than 7 potting conditions on 7 treatments. Growth parameters such as number of leaves, area leaf, length of shoots, and number of shoots were measured during 4 stage of growth. Results indicated that increase in drought stress caused many effects on morphology and enzymes activities also decrease on growth, number of leaves, leaf area and increase in growth of root rather than shoot. Drought stress did not caused significant difference in the number of shoots. Increase in drought stress also lead to increase in glycine betaine (GB), and proline content in root and leaf. Increase in proline and GB content in leaf was higher than root. Drought stress also caused increase in catalase activity in root and leaves, and more stress also lead to more activity. Drought caused increase in peroxidase activity in leaves and root, but increases in this activity by increasing stress were only observed for root. Superoxide dismutase activity in leaves was not affected significantly by drought stress, but increased in root, by increase in stress. Manuscript profile -
Open Access Article
43 - Prevalence of hydrogen peroxide contamination in sterilized milk samples distributed in Isfahan market
مریم میرلوحی محمد باقر ملجئی آذین پور خلیلیIntroduction: Hydrogen peroxide is commonly used as disinfectant for sterilization of food contact materials. In dairy industry, contamination of milk with H2O2 residue is likely due to its leakage from the disinfected surfaces resulting in development of oxidative spoi MoreIntroduction: Hydrogen peroxide is commonly used as disinfectant for sterilization of food contact materials. In dairy industry, contamination of milk with H2O2 residue is likely due to its leakage from the disinfected surfaces resulting in development of oxidative spoilage of milk. Since no report has been documented on the prevalence of such contamination in food stuff in Iran, this study aimed to measure the prevalence of H2O2 in sterilized milk samples, collected from Isfahan market in 2013. Methods: A total of 20 tetra-pack sterilized milk samples from 7 different brand were randomly collected from the market. All the samples were 250ml in volume. In order to measure the H2O2 concentration a colometric method was adapted based on catalase reaction and measurement of light absorption at 400nm. Results: H2O2 residue was detected in all tested samples ranging from 0.14-1.62 ppm. Fifteen samples (75%) contained H2O2 levels greater than its regulated level by international organization (0.5 ppm). Accordingly, the results of one-sample t-test showed that the average amount of detected H2O2 concentration in the tested sample is higher than the standard value. Conclusion: Regarding the H2O2 contamination of the tested milk samples revealed in this study and due to lack of any regulation limit for H2O2 in sterilized milk in Iran, presentation and documentation of standards introducing reliable method for detection and monitoring of H2O2 in the sterilized milk along with its limits is highly recommended. Manuscript profile -
Open Access Article
44 - Optimization of the Extraction Process of Aqueous and Alcoholic (Ethanol) Extracts of Allium Ampelloprasuml Plant by Response Surface Methodology and Its Antioxidant Effect on Soybean Oil Stability over Shelf Life
الهام Azadfar Maryam Sabetghadam زهره Bahrami بیتا BeyzaeiPlants contain phenolic compounds ranging from simple molecules such as phenolic acids to highly polymerized molecules such as tannins Phenols and polyphenols in plant tissues have gained increasing scientific interest because of their potential beneficial effects on hu MorePlants contain phenolic compounds ranging from simple molecules such as phenolic acids to highly polymerized molecules such as tannins Phenols and polyphenols in plant tissues have gained increasing scientific interest because of their potential beneficial effects on human health..In this study, Allium ampelloprasum L plant was prepared and homogenized from Sabzevar. The extraction was performed by the ultrasound extraction method using ethanol and water solvent. The amount of and antioxidant compounds of extracts was investigated by and DPPH methods, respectively. The results of the optimization process have shown that the optimum conditions of alcoholic extraction were determined as 17.81 minutes with the concentration of 800. Also, the optimum conditions for the aqueous extraction were determined as the concentration of 800 ppm and the time of 13.52 minutes. According to the optimization results, the extraction amount of antioxidant compounds in aqueous and alcoholic extractions were reported as 33.893-33.499, respectively. The results of the oxidative stability of oil have shown that the peroxide and thiobarbituric acid index in water and alcoholic extraction were 1.938-3.158 and 0.173-0.337, respectively. Manuscript profile -
Open Access Article
45 - Antioxidant Effect optimization of ethanol extract of Cirsium vulgare on oxidative stability of soybean oil and comparison with synthetic antioxidant (BHT)
الهام Azadfar Maryam Sabetghadam زهره Bahrami بیتا BeyzaeiVarious factors such as oxygen, light, heat, metal ions and enzymes can affect the oxidative stability of oils and lipids and finally, oxidative rancidity can occur. The application of synthetic antioxidants has been questioned to delay oxidative rancidity due to their MoreVarious factors such as oxygen, light, heat, metal ions and enzymes can affect the oxidative stability of oils and lipids and finally, oxidative rancidity can occur. The application of synthetic antioxidants has been questioned to delay oxidative rancidity due to their toxicity and carcinogenic probability. This study aims to optimize the oxidant performance of an ethanolic extract of Cirsium vulgare on oxidative stability of soybean oil and compare it with synthetic antioxidants (BHT). In this study, Cirsium vulgare extract was extracted under the influence of concentration (100-300 ppm), duration hours and temperatures . After performing different experiments on the extraction of the best extraction conditions, the best extraction conditions were determined using response surface methodology and extraction in optimum conditions. Data analysis was carried out. The results of the optimization process showed that the oxidative stability of oil showed that; time 24.0 min and concentration 100/01temperature 44/745 were determined. Results showed that the optimum sample of Cirsium vulgare in the stability of soybean oil during storage time was more effective than synthetic antioxidant (BHT), due to higher levels of phenolic compounds and antioxidant present in the optimized sample of Cirsium vulgare. Manuscript profile -
Open Access Article
46 - Investigating the effect of atmospheric pressure cold plasma on oxidation indices and microbial quality of red pepper
Samaneh Khodabandeh shahraki Mohammad Goli Sharifeh ShahiSpices are popular worldwide for increasing appetite and adding flavor and color to food, but they also carry a high microbial load. Cold plasma is a non-thermal technology and a suitable alternative to the conventional thermal methods used in the food industry, which, MoreSpices are popular worldwide for increasing appetite and adding flavor and color to food, but they also carry a high microbial load. Cold plasma is a non-thermal technology and a suitable alternative to the conventional thermal methods used in the food industry, which, with its antimicrobial properties, increases the shelf life of food without negatively affecting its quality. The purpose of this research was to investigate some qualitative and microbial properties of red pepper by a cold plasma method. The results showed that the plasma voltage had a greater effect on improving and maintaining the total phenolic content of red pepper than the duration of irradiation, so the use of cold plasma treatment with medium voltage (16.66 kV) increased the total phenolic content of red pepper compared to the voltages lower and higher were superior. The acid value, peroxide value, thiobarbituric acid, and anisidine index of the treatments were significantly higher than the control sample (p<0.05), and the control sample had the lowest oxidation indices. The total count of microorganisms, Staphylococcus aureus, and E. coli in the treated samples was significantly less than the control sample (p<0.05). According to the obtained results, the cold plasma method is suggested for food processing because it can maintain the quality of food at an acceptable level. Manuscript profile -
Open Access Article
47 - Comparison of Nanosil and hydrogen peroxide in reducing of Escherchia coli population on egg shell surface
Majid Gholami-Ahangaran Mohsen Jafarian-Dehkordi Mazyar Haj-salehiAbstract In this study the efficiency of two commercial anti-bacterial compounds comprising hydrogen peroxide and Nanosil D2 for reducing of bacterial population on egg shell surface was compared. Thus, 90 eggs were prepared and divided into 3 groups. In first group, Na MoreAbstract In this study the efficiency of two commercial anti-bacterial compounds comprising hydrogen peroxide and Nanosil D2 for reducing of bacterial population on egg shell surface was compared. Thus, 90 eggs were prepared and divided into 3 groups. In first group, Nanosil, in second group, hydrogen peroxide and in third group sterile water (as control) were sprayed on outer surface of egg shell. After 30 minutes, crushed egg shells were cultured. After preparation of serial dilution, Escherichia coli colonies were identified and counted according to usual bacteriological procedures. The results revealed there are no significant differences in amount of E. coli colonies between eggs exposed to Nanosil and hydrogen peroxide. The eggs exposed to Nanosil and hydrogen peroxide showed significant lower infectivity comparing to the control group. Thus, it seems that Nanosil can be used as a disinfectant of eggs. Manuscript profile -
Open Access Article
48 - بررسی اثر عصاره الکلی مرزه بختیاری بر زخم معده القا شده با الکل در موش صحرایی
فیروزه سقایی عبدالرسول نامجوBackground & Aim: Free radicals play an important role in the development of some diseases, including gastrointestinal ulcers. Current gastric ulcer drugs have side effects, so the tendency to use herbs and replace them with synthetic drugs has increased. In this re MoreBackground & Aim: Free radicals play an important role in the development of some diseases, including gastrointestinal ulcers. Current gastric ulcer drugs have side effects, so the tendency to use herbs and replace them with synthetic drugs has increased. In this regard, plants of the Satureja family have been studied in terms of healing and antioxidant properties. The aim of this study was to evaluate the protective effects of Satureja bachtiarica ethanolic extract on ethanol-induced gastric mucosal ulcers in rats.Experimental: Thirty rats were divided into 5 equal groups. Group 1 received 200 mg / kg of ethanolic extract of Satureja bachtiarica (EESB), group 2 received 400 mg / kg EESB, group 3 received 20 mg / kg omeprazole, group 4 received 1 mL of CMC and group 5 received 1 mL of normal saline. One hour after treatment, each animal received absolute ethanol (1ml / rat) orally by gavage. The other 30 mice were treated with oral ethanol after induction of gastric ulcer as described above, but all treatments were once a day for 14 days. At the end of both tests, ulcer index, histopathologic exams, Malonedialdehyde (MDA) and Superoxide dismutase (SOD) were measured.Results: Ethanolic extract of Satureja bachtiarica (400 mg/kg) and omeprazole had a significant effect on reducing ulcer index and increasing ulcer inhibition. It significantly preserved the gastric wall mucosa and reduced the formation of ethanol-induced gastric lesions. Also in this study a significant increase in superoxide dismutase (SOD) activity and a significant decrease in Malonedialdehyde (MDA) in gastric tissue were observed.Recommended applications/industries: The anti-ulcer effect of this plant seems to be due to the reduction of oxidative stress, which is probably due to the presence of thymol and carvacrol in the plant extract. This plant may be considered officially in the treatment and prevention of stomach ulcers in the future. Manuscript profile -
Open Access Article
49 - تاثیر گیاه Crinum glaucum A. Chev بر فعالیت آنزیمهای آنتی اکسیدانی اندوژن در استرس اکسیداتیو القا شده در یک مدل حیوانی
اولوفون میلایو اوگونرینولا اولواسی اوگونرینولا اولواتومیلولا اولاتونجی اولوسگون فاجانا موتیو کاظیم ژمیسولا سایبو حبیب بانکول آدمولا آدیوی باباجید الموBackground & Aim: The mechanism of action of Crinum glaucumbulb (Cgb) A. Chev (Amaryllidaceae) is enigmatic. Therefore, this study aimed at investigating the possible modulating properties of an aqueous extract of Cgb on endogenous antioxidant enzymes during lipopol MoreBackground & Aim: The mechanism of action of Crinum glaucumbulb (Cgb) A. Chev (Amaryllidaceae) is enigmatic. Therefore, this study aimed at investigating the possible modulating properties of an aqueous extract of Cgb on endogenous antioxidant enzymes during lipopolysaccharide (LPS)-induced oxidative stress in a rat model.Experimental: 25 male and 25 female rats were divided into five groups (n=5) each: control group; treatment group, rats were given an aqueous extract of Cgb for 7 days; induced-oxidative stress group, rats were injected with LPS for 4 hours; post-Cgb group, rats were injected with LPS for 4 hours and treated with an aqueous extract of Cgb; and pre-Cgb group, rats were given an aqueous extract of Cgb for 7 days, injected with LPS for 4 hours, and treated with an aqueous extract of Cgb for 7 days. The blood, brain, heart, lungs, liver, and kidneys were harvested for the biochemical analysis. The endogenous antioxidant enzymes (catalase, superoxide dismutase, and glutathione-S-transferase) were analysed spectrophotometrically.Results: The hallmark of LPS is its ability to decrease the activity of oxidative stress marker enzymes, as observed in this study. The pre- and post-administration of an aqueous extract of Cgb significantly (P≤0.05) reversed the damaging effect of LPS by increasing the activities of catalase and superoxide dismutase in the blood and organs of male and female rats, respectively, while plasma glutathione-S-transferase activity was inhibited.Recommended applications/industries: The aqueous extract of Cgb has modulating properties to reduce the action of LPS-induced oxidative stress on endogenous antioxidant enzymes in male and female rats. Manuscript profile -
Open Access Article
50 - اثرات آنتی بیوتیکی و آنتی اکسیدانی عصاره متانولی برگ گیاه Momordica charantia بر فشار خون ناشی از آلوکسان در موش صحرایی
ساندی افوگ الوفکه فلایی بلسین اگونپلو آدمولا اویابمی تمیدایو اوموبوال مومو یکوبو اولووافمی اوگونتیبجو آدلو آدداپوBackground & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momo MoreBackground & Aim:The plant Momordica charantia is highly valuable having a wide range of medicinal uses with tropical and subtropical regions distribution. This study was conducted to appraise the medicative properties of methanol leaf extract of Momordica charantia (MEMC) on alloxan-induced diabetic rats. Experimental: The antidiabetic influence of methanol leaf extract of Momordica charantia (MEMC) was investigated in alloxan-induced diabetes in rats. 50 rats allocated into five groups (A-E) (n =10) were utilized in this study: group A was normal control, groups B to E were induced with alloxan with diabetes established, while group B was not treated, group C received glibenclamide treatment while groups D and E were administered extracts at 200 and 400 mg/kg doses, respectively, in a study that continued for 28 days. Changes in blood glucose levels, body weight, haematology, serum chemistry, antioxidant system and histopathology changes were evaluated. Results: MEMC elicited significant drop in blood glucose level from diabetic to near normal level, restoring the body weight, haematological and serum biochemical parameters to the basal non diabetic level; likewise the MEMC-treated group elicited a decreased level of oxidant markers such as malondialdehyde (MDA) and hydrogen peroxide (H2O2) but increased concentration of protein thiols (PT), non-protein thiols (NPT), glutathione (GSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and superoxide dismutase (SOD) indicating its anti-oxidant potential. The pancreas section also revealed repair of distorted pancreatic architecture in MEMC-treated group compared to diabetic group. Recommended applications/industries: The plant exhibited anti-hyperglycaemic, anti-diabetic and anti-oxidant abilities hence could be explored for the treatment of diabetes mellitus. Manuscript profile -
Open Access Article
51 - Process Optimization and Kinetic study of Wet Peroxide Oxidation of Phenol in Wastewater over Mg-Al Nano Mixed Oxide
Masoud Samandari Afshin Taghva Manesh Seyed Ali Hosseini Sakineh Mansouri -
Open Access Article
52 - Alkanedisulfamic acid functionalized silica-coated magnetic nanoparticles as a reusable efficient nanocatalyst for synthesis of gem-dihydroperoxides and 1,2,4,5-tetraoxanes
Kaveh Khosravi Ali Reza Karimi Shirin Naserifar -
Open Access Article
53 - Oxidative desulfurization of light fuel oil by using hydrogen peroxide in the presence of acetic acid catalyst
Neda Ghaeli Mohammad Hassan Vakili -
Open Access Article
54 - Urea-hydrogen peroxide/silica phosphoric acid-catalyzed oxidation- condensation Tandem reaction: One-pot synthesis of 2-substituted benzimidazoles from alcohols
Ahmad Reza Momeni Heshmat Allah Samimi Reyhaneh Jahanian -
Open Access Article
55 - Thermodynamic study of an effective catalytic system, hydrogen peroxide and methyltrioxorhenium
Fatemeh Niroomand Hosseini -
Open Access Article
56 - Investigation of the Effects of Essential Amino Acids on Overexpression of the Vg and Sod Gene in Apis mellifera
S.M. Mortazavi م. چمنی M. Amin-Afshar A.A. Sadeghi G. Tahmasbi -
Open Access Article
57 - Nanoparticles induced antioxidative compounds in Matricaria chamomilla
Delaram Torabzadeh Halimeh Hassanpour Jinous Asgarpanah Maryam Rezayian -
Open Access Article
58 - Sodium nitroprusside and salicylic acid decrease antioxidant enzymes activity in soybean
Leila Aalam Mohammad Sedghi Omid Sofalian -
Open Access Article
59 - Effect of vermicompost and mycorrhiza fungi on yield and growth of milk thistle and antioxidant system activity.
Foroozan Nikkah Naeeni Ali Reza Ladan Moghadam Pezhman Moradi Mehdi Rezaei Vahid Abdoosi -
Open Access Article
60 - The Role of signaling of hydrogen peroxide and 24-epibrassinosteroid on physiological traits of cumin (Cuminum cyminum L.) under drought stress
Nasim Roudbari Hosein Abbaspour Khosrow Kalantari Ahmad Aien -
Open Access Article
61 - Physiological responses of peppermint (Mentha piperita L.) to plant growth regulators and salinity stress
Ammar Fathi Meysam Oveysi Mohammad Nasri Hamidreza Tohidi Pourang Kasraei -
Open Access Article
62 - Responses of tepary bean plant under change in growth condition
Tabasom Ghadimian Hamid Madani Nourali Sajedi Masoud Gomarian Saeed Chavoshi -
Open Access Article
63 - Antioxidant enzymes response to medicinal plant of Bakhtiari savory Satureja bachtiarica Bunge.affected by density and organic fertilizers in dryland farming conditions
Ahmad Mirjalili Mohammad Hossein Lebaschi M.Reza Ardakania Hossein Heidari Sharifabad Mehdi Mirza -
Open Access Article
64 - Hydrogen peroxide promotes metabolic changes and alleviates effects of static magnetic field on tobacco cells
Faezeh Ghanati Farzaneh Mohammadi Mohsen Sharifi Mohammad Pormehr Abazar Hajnorouzi Atefeh Payez -
Open Access Article
65 - Effect of foliar application of Cycocel and some micronutrients on activity of antioxidant enzymes of Triticum aestivum under drought stress
Saeed Akbarimehr Saeed Sayfzadeh Nasser Shahsavari Seyed Alireza Valadabadi Esmaeil Hadidi Masouleh -
Open Access Article
66 - Effects of Boron Priming On The Chemicals And Antioxidant Of Quinoa seedling
Ali Mansouri Heshmat Omidi Amir Bostani -
Open Access Article
67 - Peroxide isozymes and Malondialdehyde content and ascorbate peroxidase activity in Berberis integerrima and Cercis siliquastrum under Cd-induced stress .
Leila Hakimi Esmail Khosropour -
Open Access Article
68 - Effects of phosphorus on antioxidant system in pepper cultivars under saline conditions.
Behrokh Daei-hassani Nader Chaparzadeh Leila Sartibi Masoumeh Abedini -
Open Access Article
69 - Evaluation of proline content and enzymatic defense mechanism in response to drought stress in rice
Borzo Kazerani Saied Navabpour Hossein Sabouri Seyedeh Sanaz Ramezanpour Khalil Zaynali Nezhad Ali Eskandari -
Open Access Article
70 - تاثیر نیترات سریم و اسید سالیسیلیک بر عمر گلجایی و سیستم آنتیاکسیدانی گلهای شاخه بریده لیسیانتوس (Eustoma grandiflorum cv. Pink Picotte)
فیروزه پورزرنگار داورد هاشم آبادیبهمنظور کاهش ضایعات پس از برداشت گلهای شاخه بریده لیسیانتوس رقم ’Pink Picotte‘ آزمایشی در قالب طرح کاملا تصادفی در سه تکرار اجرا شد. تیمارهای آزمایشی شامل اسید سالیسیلیک (SA) (50، 100 و 200 میلیگرم /لیتر) و نیترات سریم (20، 40، 80 و 200 میکرومولار) بودند Moreبهمنظور کاهش ضایعات پس از برداشت گلهای شاخه بریده لیسیانتوس رقم ’Pink Picotte‘ آزمایشی در قالب طرح کاملا تصادفی در سه تکرار اجرا شد. تیمارهای آزمایشی شامل اسید سالیسیلیک (SA) (50، 100 و 200 میلیگرم /لیتر) و نیترات سریم (20، 40، 80 و 200 میکرومولار) بودند که بهصورت پالس 24 ساعته بههمراه ساکارز 3 درصد استفاده شدند. نتایج نشان داد که اثر SA و نیترات سریم در همه صفات ارزیابی شده بهجز ماده خشک معنیدار است. تیمارهای 40 میکرومولار نیترات سریم (42/15 روز) و 100 میلیگرم / لیتر SA (20/15 روز) موفقترین تیمارها در افزایش عمر گلجایی بودند. این تیمارها علاوه بر عمر گلجایی در جلوگیری از کاهش وزن تر، کاهش جمعیت باکتریایی محلول گلجایی و افزایش کلروفیل برگ برترین تیمارها بودند. بیشترین فعالیت آنزیم کاتالاز متعلق به 40 میکرومولار نیترات سریم (8.57 IU g-1 FW min-1) بود که از نظر آماری با تیمارهای 50 و 100 میلیگرم در لیتر SA اختلاف معنیداری نداشت. همچنین این تیمارها در افزایش فعالیت آنزیم SOD موثر بودند. در این پژوهش کاربرد 200 میلیگرم / لیتر SA اثر منفی بر عمرگلجایی و صفات وابسته به آن داشت. در کل کاربرد غلظتهای بالای نیترات سریم (80 و 200 میکرومولار) و اسید سالیسیلیک (200 میلیگرم در لیتر) در محلول گلجایی گلهای شاخه بریده لیسیانتوس رقم ’Pink Picotte‘ توصیه نمیشود. Manuscript profile -
Open Access Article
71 - روشهای مختلف کاربرد پلی آمینها(پوتریسین، اسپرمین، اسپرمیدین) بر برخی خصوصیات مورفولوژیک، فیزیولوژیک، آنزیمی و عمر پس از برداشت گل رز رقم dolcevita
الهام دانایی وحید عبدوسیبه منظور مطالعه روشهای مختلف کاربرد پلی آمینها(پوتریسین، اسپرمین، اسپرمیدین) بر برخی خصوصیات مورفولوژیک، فیزیولوژیک، آنزیمی و عمر پس از برداشت گل رز رقم Dolce Vita، آزمایشی به صورت آزمایش فاکتوریل در قالب طرح آماری کاملاً تصادفی با 32 تیمار، 3 تکرار و هر تکرار حاوی 5 Moreبه منظور مطالعه روشهای مختلف کاربرد پلی آمینها(پوتریسین، اسپرمین، اسپرمیدین) بر برخی خصوصیات مورفولوژیک، فیزیولوژیک، آنزیمی و عمر پس از برداشت گل رز رقم Dolce Vita، آزمایشی به صورت آزمایش فاکتوریل در قالب طرح آماری کاملاً تصادفی با 32 تیمار، 3 تکرار و هر تکرار حاوی 5 گلدان، در مجموع 480 گلدان اجرا شد. عامل اول پوتریسین، اسپرمین، اسپرمیدین با سطوح صفر، 50 ، 100 میلیگرم در لیتر و عامل دوم روش کاربرد ترکیبات بود. نانو ذرات نقره(3 میلیگرم بر لیتر) به همراه ساکارز 3% و آب مقطر به عنوان شاهد بکاررفتند. در روش اول دو هفته پیش از برداشت، گلها با سطوح مختلفی از پلی آمینها محلولپاشی شدند و سپس گلها برداشت و در آزمایشگاه در نانو ذرات نقره به همراه ساکارز(محلول نگهدارنده) تا پایان عمر ماندگاری قرارگرفتند. در روش دوم گلها پس از برداشت، با همان غلظت از پلی آمینها محلولپاشی شدند و سپس به محلول نگهدارنده تا پایان عمر ماندگاری انتقال یافتند. در روش سوم تیمار کوتاه مدت(24 ساعت) گلها پس از برداشت، با همان غلظت از پلی آمینها انجام و سپس گلها به محلول نگهدارنده تا پایان عمر ماندگاری منتقل شدند. در روش چهارم تیمار بلند مدت گلها پس از برداشت، با همان غلظت از پلی آمینها تا پایان عمر ماندگاری انجام شد. صفاتی مانند وزن تر نسبی، شاخص ثبات غشاء سلول، آنتوسیانین گلبرگ، کلروفیل کل برگ، پروتئین، فعالیت آنزیمهای فنیلآلانین آمونیالیاز و سوپراکسید دیسموتاز و عمر پس از برداشت گلها ارزیابی گردید. تیمار Spm100ppm در روش محلولپاشی پیش از برداشت بیشترین میزان پروتئین، فعالیت آنزیمهای فنیلآلانین آمونیالیاز و سوپراکسید دیسموتاز را داشت. لذا با توجه به نتایج حاصل از این پژوهش میتوان روش محلولپاشی پیش از برداشت پلی آمینها را به عنوان بهترین روش جهت بهبود دوام عمر گل رز رقم Dolce Vita توصیه نمود. Manuscript profile -
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72 - تاثیر سایتوکینین و تنش شوری بر ویژگیهای گلدهی و بیوشیمیایی یاسمن زمستانه
معصومه فاضلی داوود نادری هدف از این مطالعه بررسی اثرات سطوح مختلف تنش شوری و محلول­پاشی 6-بنزیل آمینوپورین (بنزیل آدنین) بر ویژگیهای مورفولوژیک و بیوشیمیایی یاسمن زمستانه بود. بنابراین آزمایشی در سالهای 96-1395 در مرکز تحقیقات گلخانهای دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان) انج More هدف از این مطالعه بررسی اثرات سطوح مختلف تنش شوری و محلول­پاشی 6-بنزیل آمینوپورین (بنزیل آدنین) بر ویژگیهای مورفولوژیک و بیوشیمیایی یاسمن زمستانه بود. بنابراین آزمایشی در سالهای 96-1395 در مرکز تحقیقات گلخانهای دانشگاه آزاد اسلامی واحد اصفهان (خوراسگان) انجام شد. این آزمایش بصورت فاکتوریل بر اساس طرح کاملا تصادفی با چهار سطح تنش شوری (2، 4، 6 و 8 دسیزیمنس بر متر) بهعنوان عامل اول و سطوح مختلف بنزیلآدنین (کنترل (عدم کاربرد)، 25 و 50 میلیگرم در لیتر) به عنوان عامل دوم در 3 تکرار (مجموع 36 واحد آزمایش) انجام شد. صفاتی مانند وزن تر و خشک وزن گل، تعداد گل، درصد کلروفیل a، b، کل، کاروتنوئید و آنزیم سوپراکسید دیسموتاز گیاه یاسمن زمستانه مورد مطالعه قرار گرفت. نتایج نشان داد که سطوح شوری و بنزیلآدنین تاثیر معنیداری بر تمام صفات داشت. نتایج نشان داد که فعالیت آنزیم سوپر اکسید دیسموتاز در شوری هشت دسیزیمنس بر متر بهطور معنیداری از فعالیت این آنزیم در تیمار دو دسیزیمنس بر متر بیشتر بود و بالاترین فعالیت آن در تیمار هشت دسیزیمنس بر متر مشاهده شد. از سوی دیگر، در بررسی اثرات استفاده از بنزیلآدنین، مشاهده شد که این تیمار اثرات مثبتی بر تمام صفات مورد بررسی دارد و اثرات آن­ها وابسته به غلظت است. در پایان میتوان گفت، با وجودی که تنش شوری اثر منفی بر یاسمن زمستانه داشت، تیمار بنزیلآدنین اثرات منفی تنش شوری را کاهش داده است. Manuscript profile -
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73 - تاثیر محلولهای گلجایی حاوی کبالت، سریم و نانوذرات نقره بر ماندگاری و کیفیت پس از برداشت گل های شاخه بریده پرنده بهشتی (Strelitzia reginae)
جهانگیر آذرهوش داود هاشم آبادی لیلا اسدپور بهزاد کاویانیبه ­منظور افزایش کیفیت و ماندگاری پس از برداشت گل ­های شاخه بریده پرنده بهشتی آزمایشی در قالب طرح کاملا تصادفی با 10 تیمار در سه تکرار انجام شد. تیمارهای آزمایشی شامل کلرید کبالت (250 و 500 می لی­گرم در لیتر)، نیترات سریم (100، 300 و 600 میکرومولار)، ن Moreبه ­منظور افزایش کیفیت و ماندگاری پس از برداشت گل ­های شاخه بریده پرنده بهشتی آزمایشی در قالب طرح کاملا تصادفی با 10 تیمار در سه تکرار انجام شد. تیمارهای آزمایشی شامل کلرید کبالت (250 و 500 می لی­گرم در لیتر)، نیترات سریم (100، 300 و 600 میکرومولار)، نانوسیلور (20 و 40 میلی­ گرم در لیتر) و نانوسیل (2000 و 4000 میکرومولار) بودند که بصورت پالس 24 ساعته استفاده شدند. از آب مقطر به ­عنوان تیمار شاهد استفاده شد. نتایج نشان داد که بیشترین عمرگلجایی با کاربرد 300 میکرومولار نیترات سریم (11.68 روز) بدست می ­آید که از نظر آماری با تیمارهای 100 و 600 میکرومولار نیترات سریم، 500 میلی ­گرم در لیتر کلریدکبالت و 20 میلی­ گرم در لیتر نانوسیلور تفاوت معنی­ داری نداشت. دو تیمار 300 و 600 میلی ­مولار نیترات سریم در افزایش جذب آب و ماده خشک، حفظ وزن تر، کاهش باکتری انتهای ساقه و محلول گلجایی بهترین تیمار بودند. کمترین تجمع مالون­ دی ­آلدهید (0.09 نانومول در هر گرم وزن تر) و بیشترین فعالیت آنزیم ­های پراکسیداز (0.147 نانومول در هر گرم وزن تر) و کاتالاز (1.02 نانومول در هر گرم وزن تر) با کاربرد 300 میکرومولار نیترات سریم در محلول گلجایی بدست آمد. بیشترین فلاوونوئید کاسبرگ (0.493 درصد) متعلق به تیمار 2000 میکرومولار نانوسیل بود. بیشترین کاهش وزن تر و کمترین مقادیر سایر صفات ارزیابی شده متعلق به تیمار شاهد بود. با توجه به نتایج حاصل، محلول گلجایی حاوی 300 میکرومولار نیترات سریم و 3 درصد ساکارز جهت حفظ کیفیت و افزایش ماندگاری پس از برداشت گل های شاخه بریده پرنده بهشتی توصیه می ­شود. Manuscript profile -
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74 - تاثیر محلولپاشی سیلیکون بر واکنشهای فیزیولوژیکی داوودی (Dendranthema ×grandiflorum) در دو مرحله رشد مختلف
هادی حاجی زاده زهره جبارزاده این پژوهش برای ارزیابی واکنش های فیزیولوژیکی داوودی به محلولپاشی سیلیکون در شرایط بدون تنش انجام شد. این پژوهش به صورت دو آزمایش جداگانه (در مرحله 5-4 برگی و 12-8 برگی) به صورت فاکتوریل در قالب طرح کاملا تصادفی با دو فاکتور نوع سیلیکون (در دو نوع: سیلیکات سدیم و س More این پژوهش برای ارزیابی واکنش های فیزیولوژیکی داوودی به محلولپاشی سیلیکون در شرایط بدون تنش انجام شد. این پژوهش به صورت دو آزمایش جداگانه (در مرحله 5-4 برگی و 12-8 برگی) به صورت فاکتوریل در قالب طرح کاملا تصادفی با دو فاکتور نوع سیلیکون (در دو نوع: سیلیکات سدیم و سیلیکات کلسیم) و غلظت سیلیکون (در 5 غلظت صفر، 50، 100، 150 و 200 میلی گرم در لیتر) در محیط کشت کوکوپیت- پرلیت (به نسبت حجمی 1:1) با 4 تکرار و 2 مشاهده انجام شد. ویژگی های فیزیولوژیکی نظیر فعالیت آنزیم های کاتالاز، آسکوربات پراکسیداز، گایاکول پراکسیداز، میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن اندازه گیری شدند. نتایج این پژوهش نشان داد که محلولپاشی با سیلیکات سدیم و کلسیم، میزان مالون دی آلدئید و فعالیت آنزیم های کاتالاز، آسکوربات پراکسیداز و گایاکول پراکسیداز را افزایش داد ولی پرولین و پراکسید هیدوژن کاهش یافتند. در کل، در مرحله 12-8 برگی، غلظت های زیاد سیلیکات سدیم (150 و 200 میلی گرم در لیتر) در افزایش فعالیت آنزیم های آنتی اکسیدانی و کاهش میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن موثرتر بود. در مرحله 5-4 برگی، سیلیکات سدیم با غلظت 100 میلی گرم در لیتر، فعالیت آنزیمهای آنتی اکسیدانی را افزایش داد و سیلیکات کلسیم با غلظت 150 میلی گرم در لیتر، میزان پرولین، مالون دی آلدئید و پراکسید هیدروژن را کاهش داد. Manuscript profile -
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75 - اثرات 1- متیلسیکلوپروپن و اتیلن بر فعالیت آنزیمهای آنتیاکسیدانت و صفات فیزیولوژیکی – بیوشیمیایی گل شاخه بریده میخک رقم ‘Fortune’
اعظم رنجبر نورالله احمدی ملیحه افتخاریگل میخک (Dianthus caryophyllus L.) از مهمترین گلهای شاخهبریده در سراسر دنیا محسوب میشود. بیشتر ارقام میخک حساس به اتیلن بوده و پس از برداشت دچار تغییرات فیزیولوژیکی و بیوشیمیایی متعددی میشوند. امروزه استفاده از مواد بازدارنده بیوسنتز و عمل اتیلن جهت حفظ کیفیت و افزا Moreگل میخک (Dianthus caryophyllus L.) از مهمترین گلهای شاخهبریده در سراسر دنیا محسوب میشود. بیشتر ارقام میخک حساس به اتیلن بوده و پس از برداشت دچار تغییرات فیزیولوژیکی و بیوشیمیایی متعددی میشوند. امروزه استفاده از مواد بازدارنده بیوسنتز و عمل اتیلن جهت حفظ کیفیت و افزایش عمر پس از برداشت محصولات اهمیت خاصی دارند. در این تحقیق به منظور بررسی اثر تیمار 1- متیلسیکلوپروپن و اتیلن روی حفظ کیفیت و افزایش عمر گلجای گلهای شاخه بریده میخک رقم ʼفورچونʻ آزمایشی در قالب طرح کاملا تصادفی با سه تکرار انجام شد. ابتدا گلهای شاخهبریده با چهار سطح صفر، نیم، یک و یک و نیم میکرو لیتر بر لیتر 1- متیلسیکلوپروپن به مدت 24 ساعت تیمار شدند و سپس به مدت 16 ساعت در معرض غلظت یک میکرو لیتر بر لیتر اتیلن قرار گرفتند.تجزیه و تحلیل دادهها با استفاده از نرمافزار آماری MSTATC و مقایسه میانگینها بر اساس آزمون LSD در سطوح احتمال یک درصد انجام شد. نتایج این تحقیق نشان داد که تیمار 1- متیلسیکلوپروپن اثر معنیداری بر روی صفت عمر گلجایی و همچنین صفات بیوشیمیایی از جمله میزان کلروفیل برگ، میزان آنتوسیانین گلبرگ، ثبات غشا گلبرگ و میزان فعالیت آنزیمهای آنتیاکسیدانت از جمله کاتالاز، پراکسیداز و سوپراکسید دیسموتاز داشت. بالاترین عمر گلجایی و بیشترین ثبات غشا مربوط به تیمار یک و نیم میکرو لیتر بر لیتر 1- متیلسیکلوپروپن بود که بر اساس آزمون LSD تفاوت معنیداری در سطح یک درصد با تیمار شاهد و غلظت نیم میکرو لیتر بر لیتر 1- متیلسیکلوپروپن داشت، اگرچه با تیمار یک میکرولیتر بر لیتر 1- متیلسیکلوپروپن دارای تفاوت معنیداری از لحاظ آماری نبود. بیشترین مقدار کلروفیل و آنتوسیانین در تیمار 5/1 میکرو لیتر بر لیتر1 - متیلسیکلوپروپن بدست آمد که اختلاف معنیداری در سطح احتمال 1 درصد با سایر تیمارها نشان داد. همچنین بیشترین و کمترین فعالیت آنزیم کاتالاز و پراکسیداز به ترتیب مربوط به تیمار 1- متیلسیکلوپروپن با غلظت یک میکرو لیتر بر لیتر و تیمار شاهد بود، بیشترین و کمترین فعالیت آنزیم سوپراکسید دیسموتاز به ترتیب در تیمارهای 1- متیلسیکلوپروپن با غلظت یک و نیم میکرو لیتر بر لیتر و تیمار شاهد مشاهده شد. درنتیجه، کاربرد 1- متیلسیکلوپروپن شروع علایم پیری را به تاخیر انداخته و باعث افزایش عمر گلجایی گلهای شاخه بریده میخک ’فورچون‘ شد. Manuscript profile -
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76 - Effect of seed priming with hydrogen peroxide on reducing the effects of saline water on bread wheat lands (Triticum aestivum L.)
Zahra Albaji Seyed Keyvan MarashiThis research was performed as a factorial in a completely randomized design in a pot with four replications. The studied treatments include salinity of irrigation water as irrigation with ordinary water (0.4) (control) and with salinity of 4 and 8 dS/m and seed priming MoreThis research was performed as a factorial in a completely randomized design in a pot with four replications. The studied treatments include salinity of irrigation water as irrigation with ordinary water (0.4) (control) and with salinity of 4 and 8 dS/m and seed priming including no priming and seed priming in 25 and 50 mM of hydrogen peroxide solutions. In this experiment, the number of spikes per pot, number of spikelets per spike, number of seeds per spikelet, 1000-grain weight, root dry weight, grain yield and grain protein were investigated. In this study, the effect of salinity of irrigation and hydrogen peroxide on all studied traits were significant. The interaction effect of salinity of irrigation and hydrogen peroxide on the studied traits except grain number per spikelet and grain protein were significant. The maximum root dry matter (0.95 g/pot) and grain yield (7.49 g/pot) were observed under irrigation with normal water and application of 50 mM hydrogen peroxide and the minimum root dry matter (0.82 g/pot) and grain yield (3.17 g/pot) was obtained at salinity of 8 dS/m and non-application of hydrogen peroxide. The maximum grain protein (14.25%) was obtained in the application of 50 mM hydrogen peroxide and the minimum protein percentage (11.05%) was obtained under irrigation with normal water. In general, the application of hydrogen peroxide can be effective in increasing the quantitative and qualitative yield of wheat and reducing the effect of salinity of irrigation water and it can be considered and recommended by researchers and farmers. Manuscript profile -
Open Access Article
77 - Study of defense mechanism of microalgae Haematococcus pluvialis infected by Paraphysoderma sedebokerensis
Bahareh Nahidian Faezeh Ghanati Maryam Shahbazi neda Soltani Morteza GholamiIn the present study, the first early physiological responses of the green micro algae Haematococcus pluvialis during infection by chytrid Paraphysoderma sedebokerensis was studied. To this end, the palmeloid form of Haematococcus pluvialis was cultivated for two days i MoreIn the present study, the first early physiological responses of the green micro algae Haematococcus pluvialis during infection by chytrid Paraphysoderma sedebokerensis was studied. To this end, the palmeloid form of Haematococcus pluvialis was cultivated for two days in three media including the medium infected with chytrid (chyt) and the culture of chytrid infected (IBS) and uncontaminated (HBS) cells. Then, the activity of antioxidant enzymes, extracellular peroxide, and free intracellular enzymes were determined by UV/Vis spectrophotometry and HPLC techniques. The activity of superoxide dismutase, catalase, and peroxidase in the chytrid infected cells after 24, 24, and 48 h was 2.3, 6.7, and 2.6 times higher those of control. These values for the cells cultivated with the infected media after 12, 12, and 36 h was found to be 2.1, 2.5, and 2.6 times higher than control. The extracellular peroxide content in the algae cultivated with the contaminated media was nearly constant and about 4 fold of the other specimens; while those of chytrid infected cells was slightly increased from 1.3 to 1.8 μM during 48 h cultivation. Amino acids histidine, alanine, asparagine, aspatic acid, arginine, and methionine were of the highest and the phenylalanine and tryptophan were of the lowest content. These results can be attributed to the cells defense response to chytrid infection thorough enhancement of peroxide content and activation of antioxidative pathways. Manuscript profile -
Open Access Article
78 - Optimizing oxidative desulfurization of diesel of Tehran Refinery using acid catalysts in the presence of hydrogen peroxide
mahsa khorshidnia Amir Farshi Manocher BahmaeiIn this research, desulfurization of diesel from Tehran Refinery was investigated by oxidation method. To investigate this process, the performance of different advanced oxidation systems (acetic acid/hydrogen peroxide, formic acid/hydrogen peroxide) along with acetonit MoreIn this research, desulfurization of diesel from Tehran Refinery was investigated by oxidation method. To investigate this process, the performance of different advanced oxidation systems (acetic acid/hydrogen peroxide, formic acid/hydrogen peroxide) along with acetonitrile extraction were investigated for desulfurization of diesel fuel with 13370 ppm primary sulfur in a discontinuous reactor. The effect of different operating factors such as molar ratio of acid to sulfur (nacid/ns) (15, 20, and 25), molar ratio of hydrogen peroxide (oxidant) to sulfur (no/ns) (5, 10, and 15), temperature and also reaction time were investigated. Many tests showed that in the optimal conditions for removing sulfur from gas oil with the acetic acid/hydrogen peroxide system, the amount of sulfur reached to 9130 ppm with an efficiency of 31%, and with the formic acid/hydrogen peroxide system, the amount of sulfur reached to 2500 ppm with an efficiency of 81%. Also, the examination of the experiments confirmed this point that it is not possible to completely remove the sulfur from heavy oil fractions such as diesel with normal oxidative desulfurization methods. Manuscript profile -
Open Access Article
79 - Effect of hydrogen peroxide based on silver colloid on decapsulation of Urmia Lake Artemia cysts (Artemia urmiana) to achieve the best hatching rate
R. Mosavi Nodoushan S. FarahnakThis study aimed to determine the most appropriate dose of hydrogen peroxide based on silver colloid for the decapsulation of Urmia Lake Artemia cysts (Artemia urmiana), to achieve the best hatching rate. The cysts were exposed to doses of 0.5, 0.75, 1, 2 and 5% o MoreThis study aimed to determine the most appropriate dose of hydrogen peroxide based on silver colloid for the decapsulation of Urmia Lake Artemia cysts (Artemia urmiana), to achieve the best hatching rate. The cysts were exposed to doses of 0.5, 0.75, 1, 2 and 5% of hydrogen peroxide based on colloidal silver for 30 seconds, 45 seconds, 1, 2 and 5 minutes and 5 ml bleach and pure water (as control) were used to compare the process of decapsulation with the test solutions. After decapsulation, 1 gram of cysts per liter were hatchedat 30 ° C for 48 hours under conditions of 35 ppt salinity. After 48 hours of incubation, 99% hatching rate at a dose of 0.5 % solution of hydrogen based on colloid silver, 97% in bleach solution and 94 % in the control cysts was observed. According to the results of the statistical analysis, ANOVA, significant difference was found between the results of test treatments. The results of this study suggest that, best performance of hatching rate in Artemia urmiana cysts can be achieved, by putting the cysts in a solution with a dose of 0.5% of hydrogen peroxide based on silver colloid. Manuscript profile -
Open Access Article
80 - Effect of Al antioxidiant enzymes activity,nitratereductase activity, amount of osmolite and release of ammonium in Dunaliella salina teodoresco
A. Sateei M. Ghorbanli F. YadollahiAluminum effect with 0, 100, 200, 300, 400, 500 and 1000 µ molar concentrations was considered during ten days on Dunaliella salina teodoresco antioxidant enzymes, smolit contents,nitrate reductase activity and ammonium release on were measured. The results showed MoreAluminum effect with 0, 100, 200, 300, 400, 500 and 1000 µ molar concentrations was considered during ten days on Dunaliella salina teodoresco antioxidant enzymes, smolit contents,nitrate reductase activity and ammonium release on were measured. The results showed that ammonium release, peroxidase , nitrate reductase activity and glycinebetaine content had not any significant differences but catalase,superoxide dismutase(300 µmolar concentration) activities,and prolin content decreased and ascorbate peroxidase activity increased significantly.Based on the results of this study, the green alga Dunaliella salina teodoresco green alga can tolerate Al stress by ascorbate peroxidase activity increase. Manuscript profile -
Open Access Article
81 - Relation of water deficit stress and biofertilizer on some of antioxidant enzymes activity and role them on grain yield variation of barley (Hordeum vulgare)
Mohammadreza DadniaTo evaluate the qualitative traits of barley in response to biofertilizers (Azotobacter, Pseudomonas and Azospirillum) with affected by different regimes of irrigation the experiment was carried out in Karaj Azad University research field in 2013 with split plot based o MoreTo evaluate the qualitative traits of barley in response to biofertilizers (Azotobacter, Pseudomonas and Azospirillum) with affected by different regimes of irrigation the experiment was carried out in Karaj Azad University research field in 2013 with split plot based on Completely Randomize Block Design with four replications. In this experiment irrigation treatments were in main plots with three levels which interrupt of irrigation at 80, 65 and 50% of field capacity (normal irrigation), (35 and 50 percent of humidity discharge) and sub plots contained seed inoculation with bacteria such as |inoculation with Azospirillum lipoferum, Azotobacter chrococcum, Pseudomonas putida, Pseudomonas×Azotobacter, Pseudomonas×Azospirillum, Azotobacter×Azospirillum | and control (un inoculation) in seven levels. The results of analysis of variance showed that biofertilizer had significant effect on antioxidant enzymes at 1% probability. The activity of antioxidant enzymes affected with biofertilizers were increased at water deficit condition which Azospirillum lipoferum caused increasing about 16.7% and 21.4% and Pseudomonas×Azospirillum caused increasing about 18.2% and 25.9% for superoxide dismutase and glutathione peroxidase in irrigation at 50% of field capacity, respectively than control. Yield showed low decrease in irrigation at 50% of field capacity than normal irrigation such as yield decreasing in irrigation at 50% of field capacity was only about 13.2% with affected by Azospirillum than normal irrigation. The data showed that biofertilizer have efficient role on barley yield at water deficit condition. Manuscript profile -
Open Access Article
82 - Studying the effect of irrigation regimes and superabsorbent levels on the antioxidant enzymes on the relative permeability of cell membranes in sorghum (Sorghum bicolor L.)
mansour fazeli rostampour مسعود فاضلی رستم پورIn order to evaluate the effect of irrigation regimes and superabsorbent polymer (SAP) on amount of antioxidant enzymes, leaf relative water content and relationships between them with relative permeability of cell membranes in sorghum, the experiment was conducted in D MoreIn order to evaluate the effect of irrigation regimes and superabsorbent polymer (SAP) on amount of antioxidant enzymes, leaf relative water content and relationships between them with relative permeability of cell membranes in sorghum, the experiment was conducted in Dashtak region of Zahedan during 2013 and 2014 seasons. The experimental design was a split plot with two factors including four irrigation regimes )40, 60, 80 and 100% crop evapotranspiration( as main plots, four amounts of SAP )0, 75, 150 and 225 kg ha-1( as subplots in a completely randomized block design with three replications. The results indicated that the effect of irrigation regimes, SAP levels and interaction effects of two factors were significant. The regression models estimated for superoxide dismutase, ascorbate peroxidase, glutathione peroxidase, catalase and RPCM showed that showed that by increasing the amount of irrigation and SAP, the traits were decreased. In addition, by decreasing in leaf relative water content, the antioxidant enzymes and relative permeability of cell membranes increased. The experiment findings indicate that at the application 75 kg ha-1 SAP and supplying 80% of sorghum water requirement, the relative permeability of cell membranes was 59.83, that was similar to 100% irrigation along with the application 150 and 225 kg ha-1 SAP. Manuscript profile -
Open Access Article
83 - Ions content and antioxidant response of barley to different methods of salicylic acid application under salinity conditions
Elahe Hashemi Yahya Emam Hadi Pirasteh-AnoshehAlthough it has been well-known that salicylic acid (SA) can increase salinity tolerance in barley; there is no consensus on the best method and time of application. Thus, a factorial experiment based on completely randomized design under controlled conditions was condu MoreAlthough it has been well-known that salicylic acid (SA) can increase salinity tolerance in barley; there is no consensus on the best method and time of application. Thus, a factorial experiment based on completely randomized design under controlled conditions was conducted at College of Agriculture, Shiraz University in 2014. In this research, the effect of SA at five levels: control (No SA), seed priming, foliar application at complete establishment, double ridges and anthesis was examined on grain yield, sodium (Na+ ) and potassium (K+ ) concentration in shoot and root as well as antioxidant enzymes activities of barley cv. Reyhane under three salt stress levels: control, 6 and 12 dS m-1. The results showed that salt stress, depended to its severity reduced Na+ and increased K+ in shoot and root and enhanced antioxidant enzymes including peroxidase, catalase, ascorbic peroxidase and superoxide dismutase, so that the most severe salt stress caused 147.4% increase in Na+ and 44.1% reduction in K+ . Nevertheless, SA in priming and foliar application could compensate some of these changes and so increased grain yield. For example, seed priming and foliar application at complete establishment, double ridges and anthesis increased K+ by 72.7%, 77.3%, 22.7% and 18.2% and decreased Na+ by 37.8%, 40.5%, 27.0% and 13.5%, respectively. Overall, it can be concluded that the highest positive effect f SA was observed in seed priming and foliar application at establishment, which showed that SA might induce more salt stress tolerance if applied at early growth stages. Manuscript profile -
Open Access Article
84 - Effect of spermidine and application on internal polyamine content, free radical enzyme activity and lipid oxidative destruction in cut ‘Black Magic’ rose flowers.
REza Namatolah Sani Seyed Hosein Nemati Mahmoud Shour Mohammad Farjadi-ShakibRose is one of the most important ornamental plants which nowadays holds the first top position in production and export of cut flowers due to its beautiful and colorful flowers. The quantity and quality of rose cultivars depend on environmental and nutrition. Pre and p MoreRose is one of the most important ornamental plants which nowadays holds the first top position in production and export of cut flowers due to its beautiful and colorful flowers. The quantity and quality of rose cultivars depend on environmental and nutrition. Pre and post-harvest application of plant growth regulators is one of the common methods for quality improvement of this flower. Therefore, in the present study, cut ‘Black Magic’ rose flowers were treated at pre harvest with spermidine (0, 50 and 100 mgl-1) and with salicylic acid (0, 50 and 100 mgl-1) during postharvest. Consequently, postharvest quality from various point of view such as internal polyamine content, activity of free radical enzymes (such as superoxide dismutase, catalase and polyphenol oxidase) and destructive lipid oxidation (malondialdehyde content) were studied. Results indicate that foliar application with spermidine and with salicylic acid increases petal soluble protein content, improves the activity of superoxide dismutase, catalase and poly phenol oxidase in the petals of cut ‘Black Magic’ rose flowers. Activity increment of enzymes that are involved in oxidative stress, decreased lipid peroxidation and consequently, they decreased malondialdehyde content as destructive biomarker. Internal polyamines such as spermine, spermidine and putrescine were also significantly affected by spermidine and salicylic acid application. Manuscript profile -
Open Access Article
85 - The Sanitizing Effect of Peracetic Acid on Microbial Contamination of Pistachio (Pistacia vera L.)
Najmeh Pakdaman Mohammad Moradi Ghahdarijani Amanollah Javanshah Ahmad Shakerardekani Najmeh Saberi -
Open Access Article
86 - Determination of the Best Drying Temperature of Hazelnut (Corylus avellana) after Six Months of Storage
Milad Mousadoost Alireza Eslami Zahra Yousefi -
Open Access Article
87 - Determination of Nutritional Value and Oxidative Stability of Fresh Walnut
Najmeh Chatrabnous Navid Yazdani Kourosh Vahdati -
Open Access Article
88 - Plant Growth Regulators Improve Yield and Yield Components of Wheat under Deficit Irrigation Treatments With Considering the Productive Capacity
MOHAMMAD HASHEM AZIZI ALI SOLEYMANI Ali Soleymani HAMID REZA JAVANMARD -
Open Access Article
89 - The Types of Plant Growth Regulators on Grain Yield and Yield Components of Barley (Hordeum vulgare L.) in Water Stress Condition
MOHAMMAD REZA ASKARNEJAD ALI SOLEYMANI HAMID REZA JAVANMARD -
Open Access Article
90 - The effect of lead and copper on some physiological traits in plant species of grass pea (Lathyrus sativus (
Mahtab Beladi Ali Kashani Davoud Habibi Farzad Pak nezhad Mahya GolshanSome plant species are tolerant to environmental adverse conditions so that with their functional mechanisms prevent more production of free radicals of oxygen or counter with produced free radicals of oxygen. This study was done to investigate the effect of lead and co MoreSome plant species are tolerant to environmental adverse conditions so that with their functional mechanisms prevent more production of free radicals of oxygen or counter with produced free radicals of oxygen. This study was done to investigate the effect of lead and copper on Lathyrus sativus in contaminated soils with these elements. To evaluate and recognize the study species ability against heavy metals lead and copper an experiment was carried out on Lathyrus sativus cultivar Zanjan in 2009. The experiment was carried out factorially as randomized complete design with four levels of lead Pb (NO3)2 (0, 200, 400 and 800 mg/kg) and four levels of copper Cu (So4)2 (0, 150, 300, 450 mg/kg) were used.Although the results suggested the decrease in total chlorophyll content and lipid membrane under the toxicity of lead and copper, the more activity of the enzyme Superoxide dismutase (SOD) along with the increase in the levels of lead and copper in thid species prevented the more production of free radicals of oxygen in the study species, so that a negative coloration was observed between chlorophyll a, b and total chlorophyll and the enzyme superoxide dismutase (SOD). Also the coloration between Malondialdehyde (MDA) which is a means for measuring the lipid per oxidation process, and the enzyme Superoxide dismutase (SOD) was positive that means the increase in this biomarker content which is a sign of more production of oxygen for lipid membrane destruction, was accomplished with the more activity of enzyme Superoxide dismutase (SOD) for more digestion and elimination of destructive oxygen. Also the water condition in leaf tissues (RWC) was not effective by these elements. Manuscript profile -
Open Access Article
91 - The Effects of IBA and H2O2 on Rooting of 2 Olive Cultivars
E. Asl moshtaghi A.R. Shahsavar -
Open Access Article
92 - Effect of Hydrogen Peroxide Concentration in Remediation of Oil-contaminated Soils with Use of Fenton Reaction
A. S. Yousefi A. Bostani -
Open Access Article
93 - Effect of Tannery Effluents on the Antioxidant Enzymes of a Fresh Water Fish Channa striatus
Sivachandran Ramachandran Navaneetha Lakshmi Krishnan Priya Ponmudi -
Open Access Article
94 - Mechanisms of Antioxidant Actions and their Role in many Human Diseases: A Review
Zahraa Kamil Kadhim Lawi Feryal Ameen Merza Shiama Rabeea Banoon Mohammed Abd Ali Jabber Al-Saady Aswan Al-Abboodi -
Open Access Article
95 - Comparative Study on Cholesterol Content and Physicochemical Properties of Some Branded and Unbranded Commercial Edible Oils in Khulna, Bangladesh
Md. Hasan Md. Hossain Palash Dhar Md. Haque -
Open Access Article
96 - The Antioxidant Effect of N-Acetylcysteine on the Expression of SOD and HOG Genes in Human Dermal Fibroblast in High Glucose State
Bahareh Safavi Zeinab Piravar Mina RamezaniWound healing in diabetic patients is delayed, because of oxidative stress. This study aims at investigating the molecular changes in Human Dermal Fibroblasts (HDFs) in a high-glucose state and improving the effect of N-Acetyl-L-Cysteine (NAC) and gene expression of oxi MoreWound healing in diabetic patients is delayed, because of oxidative stress. This study aims at investigating the molecular changes in Human Dermal Fibroblasts (HDFs) in a high-glucose state and improving the effect of N-Acetyl-L-Cysteine (NAC) and gene expression of oxidative genes Super-Oxide Dismutase (SOD) and Heme Oxygenase1 (HO1). HDFs were cultured in 5.5, 25, 50, and 75 mM glucose concentrations for 72 hours. Cell proliferation was examined via 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assays. Oxidative stress markers of SOD and HO1 were quantified with real-time Polymerase Chain Reaction (PCR). The antioxidant effect of NAC on 1 mM was examined to evaluate oxidative markers in the glucose effects on the HDFs. The MTT assay revealed a decline in cell viability in 50 and 75 mM glucose concentrations. mRNA level of SOD and HO1 was upregulated. The antioxidant addition of NAC reduced the inhibitory effect of the high-glucose state on the proliferation of the HDFs. A high-glucose state impairs the in vitro proliferation and migration of HDFs and may, therefore, induce increased oxidative stress and cellular dysfunction. The antioxidant effect of NAC ameliorates the damaging impact of a high-glucose state. Manuscript profile -
Open Access Article
97 - The Effect of Scrophularia striata Hydroalcoholic Extract in the Prevention of Gastric Ulcers Induced by Indomethacin in Rats
Sh. Ferdowsi Z. Hooshmandi E. ShahmoradiA peptic ulcer is a common disease. One of the goals of therapy is using drugs with fewer side effects. This study aimed to investigate the effect of Scrophularia strata (Scrophularia) extract in the prevention of gastric ulcers induced by indomethacin in rats. Overall, MoreA peptic ulcer is a common disease. One of the goals of therapy is using drugs with fewer side effects. This study aimed to investigate the effect of Scrophularia strata (Scrophularia) extract in the prevention of gastric ulcers induced by indomethacin in rats. Overall, 64 male Wistar rats were divided into 8 groups. Experimental groups including 1- control group that received normal saline 0.9% and at the last day received 48 mg indomethacin. 2- Ascorbic acid 50 mg/kg -indomethacin treatment; 3- Omeprazole 10 mg/kg-indomethacin treatment; 4- Ranitidine 50 mg/kg-indomethacin treatment; 5- S. strata 10 mg/kg –indomethacin treatment; 6- S. strata 50 mg/kg -indomethacin treatment; 7- S. strata 100 mg/kg-indomethacin treatment; 8- S. strata 150 mg/kg-indomethacin treatment. After macroscopic examination, stomach tissue was studied to examine the enzymes catalase, glutathione peroxidase, and superoxide dismutase. Catalase and glutathione peroxidase activity levels in S. strata 50, 100 and 150 mg/kg treatment was significantly increased compared with the control group (P<0.001). The superoxide dismutase activity in S. strata 100 and 150 mg/kg treatment was significantly increased compared with the control group (P<0.001). Stomach tissue damage significantly decreased in the group receiving 100 and 150 mg/kg S. strata and group receiving ranitidine and indomethacin compared to control (P<0.001). S. strata extract increased catalase, glutathione peroxidase, and superoxide dismutase and dose-dependently prevented the stomach tissue damage (by increasing the level of endogenic antioxidant). Manuscript profile -
Open Access Article
98 - The effect of superabsorbent polymer role on quantitative and qualitative traits on wheat in soils contaminated with nickel
hadi Zarei Hamid Reza Tohidi Moghadam farshad GhooshchiIn order to study effect of super absorbent polymer application to mitigate adverse effects of nickel (Ni), a greenhouse experiment was conductedin Varamin, Iran during the growing season of 2016 in wheat. The experiment was installed in a completely randomized design, MoreIn order to study effect of super absorbent polymer application to mitigate adverse effects of nickel (Ni), a greenhouse experiment was conductedin Varamin, Iran during the growing season of 2016 in wheat. The experiment was installed in a completely randomized design, with treatments arranged in a factorial scheme with three levels of nickel (0, 60 and 120 mg per kg of soil) by three levels super absorbent polymer application (with out super absorbent polymer application (Control), 2 and 4 g per kg of soil), with three replicates. The results showed that nickel treatment significantly for all experimental traits. The nickel contamination caused a significant decreased plant height, total dry weight per plant and root dry weight per plant at by 8.51%, 31.52 and 56.06% respectively to control, Whereas, increased proline content, superoxide dismutase enzyme activity and the accumulation of nickel in leaves. Also the results showed that super absorbent polymer application increased plant height, total dry weight per plant and root dry weight per plant whereas, decreased proline content, superoxide dismutase enzyme activityand the accumulation of nickel in leaves. Generally the results of the present study indicate that super absorbent polymer application reduces the harmful effects of nickel stress and super absorbent polymer application treatment decreased oxidative stress in wheat plant Manuscript profile -
Open Access Article
99 - Effect of drought conditions and mycorrhiza on yield components and enzime activity of safflower (Carthamus tinctorius .L)
Amir Momeni Hossein Ali Sheybani Mohammad Reza MomayeziIn order to study the Effect of mycorrhiza on yield and yield components of Safflower under drought stress an experiment in split plot based on complete block design with three replications in 1392 in Varamin region was done. treatments were first factor drought in four MoreIn order to study the Effect of mycorrhiza on yield and yield components of Safflower under drought stress an experiment in split plot based on complete block design with three replications in 1392 in Varamin region was done. treatments were first factor drought in four irrigation regimes including 50 (Control), 90, and 130 mm evaporation from evaporation pan (Class A) and the second factor consisted of four levels of mycorrhiza (Control, 100, 200 and 300 mg) respectively. Results showed a significant effect of mycorrhiza on oil percentage and catalase, glutathione enzymes. Effect of drought stress on catalase, glutathione, superoxide dismutase enzymes and proline and protein and oil percentage was significant. Interactions between drought stress and mycorrhiza was significant and The Best Results in normal irrigation (control) and 200 mg treatment was observed. The highest oil content in the seed inoculation with mycorrhizal fungi 200 mg (B3) and control (B1), respectively, with an average of 28.11 and 23.44 per cent respectively. The highest and lowest catalase activity in the treatment of 130 mm evaporation Class A3 (severe stress) and 50 mm evaporation Class A1 (conventional irrigation), respectively, 16.6 and 9.7 mg protein per minute Was obtained. Most of the glutathione peroxidase activity in the treatment of severe drought stress (4.8 mg protein per minute) and the lowest in the treatment of stress (9.4 mg protein per min). Manuscript profile -
Open Access Article
100 - Effect of foliar application of selenium onquality traits and enzyme activities of safflower (Carthamus tinctorius .L) under different soil moisture regimes stress in varamin region
Behnam Khademi Hossein Ali Shaibani Arash BorzouIn order to study the effect of selenium foliar application on yield and yield components of safflower (Carthamus tinctorius.L) under different soil moisture regimes stress an experiment in split plot based on Randomized complete block design with three replications in MoreIn order to study the effect of selenium foliar application on yield and yield components of safflower (Carthamus tinctorius.L) under different soil moisture regimes stress an experiment in split plot based on Randomized complete block design with three replications in 1393 in The Islamic University research farm Varamin unit. Treatments were main plot drought in four irrigation regimes including 50 (Control), 80, and 110 mm evaporation from evaporation pan (Class A) and sub plotconsisted offour levelsof selenium (Control, 100, 200 and 300 mg/lit) respectively. Results showed a significant effect of drought stress and selenium on Catalase, Glutathione, Superoxide dismutase, Oil and protein percentage. Effect of selenium on selenium content of grain was significant but the effect of drought stress on this trait is not significant. Interactions between drought stress and selenium on selenium content of grain and oil percentage was significant and The Best Results in normal irrigation (control) and 300 mg treatment was obtained. Manuscript profile -
Open Access Article
101 - Effect of foliar application of ascorbic acid (vitamin c) and irrigation withholding in different growth stages on quantitative and qualitative characteristics and some biochemical's changes in Sunflower (Helianthus annuus L.)
Malahat Davoodi Mackinejad Hamid Reza Tohidi Moghadam Nabi-Alah NematiIn order to study the effects of water deficit stress and foliar application of ascorbic acid, an experiment was conducted in research field of Islamic Azad University, Varamin Branch in Iran during 2012 growing season. The experimental design was laid out in a randomiz MoreIn order to study the effects of water deficit stress and foliar application of ascorbic acid, an experiment was conducted in research field of Islamic Azad University, Varamin Branch in Iran during 2012 growing season. The experimental design was laid out in a randomized complete block design with a split plots arrangement in three replications. Main plots included four different levels of irrigation (complete irrigation, irrigation withholding at 8-leaf stage, irrigation withholding at grain filling stage and irrigation withholding at both 8-leaf and grain filling stages) and three different concentration of Ascorbic Acid foliar application (0, 100 and 200 ppm) was allocated to subplots. The results showed that irrigation withholding conditions in different growth stages significantly decreased seed yield, biological yield, harvest index, oil and protein percentage, oil and protein yield and total chlorophyll content but by contrast increased proline content and superoxide dismutase activity in leaves. Foliar application of ascorbic acid as antioxidant enzyme could increased quantitative and qualitative yield and chlorophyll content and decreased proline and superoxide dismutase activity in leaves by reduction of water deficit stress. Foliar application of Ascorbic Acid with 200 ppm under irrigation withholding at both 8-leaf and grain filling stages increased 38/46% yield seed compared with treatment that foliar application without Ascorbic Acid. In addition, Ascorbic Acid foliar application reduced the harmful effects of water deficit stress as antioxidant and improved growth conditions for water deficit stress. Manuscript profile -
Open Access Article
102 - The effect of super absorbent polymer application on some physiological and biochemical characteristics of wheat (Triticum aestivum. l.) under cut irrigation The effect of super absorbent polymer application on some physiological and biochemical characteristics of wheat (Triticum aestivum. l.) under cut irrigation The effect of super absorbent polymer application on some physiological and biochemical characteristics of wheat (Triticum
Ashkan Askiyani Farshad Ghooshchi Hmid Reza Tohidi MoghadamIn order to study the effects of amount of super absorbent polymer on some physiological and biochemical characteristics of wheat under normal and cut irrigation an experiment was conducted in research field of Islamic Azad University, Varamin Branch during 2012 growing MoreIn order to study the effects of amount of super absorbent polymer on some physiological and biochemical characteristics of wheat under normal and cut irrigation an experiment was conducted in research field of Islamic Azad University, Varamin Branch during 2012 growing season. The experimental design was laid out in a randomized complete block with a split plots arrangement of treatments in three replications. Main plots included four different levels of irrigation (complete irrigation or no irrigation withholding,cut irrigation at stem elongation, cut irrigation at flowering, and cut irrigation at both flowering and seed setting stages ) and sub factors were included super absorbent (0, 20 and 40 kg per hectare). The results showed that cut irrigation conditions in different growth stages significantly decreased seed yield, carbohydrate grain content, total chlorophyll content and gibberellin but increased protein percentage, superoxide dismutase activity and malondialdehyde content. Super absorbent polymer application in cut irrigation in different growth stages had positive effect on seed yield. In general, we concluded that super absorbent can improve growth conditions for wheat plants grown under water deficit stress. The reviews although show super absorbent is able to reduce the destroying effect of low water by absorbing and preserving water and approve varying agricultural characters. With attention to increased yield and its components and decreasing plant water need, using this material is economically acceptable Manuscript profile -
Open Access Article
103 - Effect of Nano-selenium particles and sodium selenite on performance, carcass characteristics and antioxidant enzymes of quails under heat stress
Ebrahim Talebi Reyhane Ghazanfarpoor This study was designed to investigate the effects of Nano-Selenium and Sodium Selenite on performance, carcass characteristics, Glutathione Peroxidase and Superoxide dismutase of Japanese quail under heat stress condition. In this investigation, 300 Japanese qua More This study was designed to investigate the effects of Nano-Selenium and Sodium Selenite on performance, carcass characteristics, Glutathione Peroxidase and Superoxide dismutase of Japanese quail under heat stress condition. In this investigation, 300 Japanese quail chicks were used and the chickens were raised to 21 days in the same condition. The experiment was performed in a randomized complete block design (RCBD) with 3 treatments and 5 replicates of 20 chicks per replicate. The chickens daily were included under heat stress condition for 8 h at 34± 0.5oC. The results showed that weight gain and feed intake of chicks fed diets with Selenium Nano particles compared to the other two groups was significant (p < 0.05) as well as the FCR between different groups was no significant difference (p>0.05). The all three different treatments showed no significant effect on carcass composition (p>0.05) and increase in use of Nano-Selenium was more tender than sodium Selenite group, but not significant. The results revealed that glutathione peroxidase and superoxide dismutase activity increased significantly in second experimental group using selenium (p < 0.05). In the present study, superoxide dismutase activity was significantly increased after adding 0.2 mg sodium selenite and nano-selenium (p < 0.05). The group of hemoglobin using selenium sources was significantly increased, but not significant increase in hematocrit. Results in this investigation revealed that Nano-Selenium is a better alternative compare to sodium Selenite. Manuscript profile -
Open Access Article
104 - Protective Effect of Coenzyme Q10 and Vitamin C on Cysteamine�Induced Lipid Peroxidation
سروش Almasi بابک Rezvanjoo S.H Shirazibeheshtiha علی Namvaran AbbasAbad مهدی khosraviBenefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and MoreBenefts of antioxidant supplementation in various disorders through reducing free radicals and improving organsperformance have been reported. The aim of this study was to investigate the effects of coenzyme Q10 and vitaminC on Cysteamine-induced lipid peroxidation and oxidative stress.This experimental interventional study was conducted on male Wistar rats. Animals were divided into four groups(six rats) randomly. Groups were treated as; group 1 (Normal saline), Group 2 (Cysteamine), Group 3 (vitaminC plus Cysteamine), Group 4 (coenzyme Q10 plus Cysteamine). 24 hours after the last injection, rats wereanesthetized and sampled for investigations. Welch’s and Kruskal-Wallis tests were used for analyzing data and P< 0.05 was set the signifcance level.The results of this study indicate that injection of cysteamine signifcantly (P < 0.05) decreased glutathioneperoxidase activity compared with control group. Pretreatment with vitamin C signifcantly (P < 0.05) increasedglutathione peroxidase activity compared with cysteamine group. Pretreatment with coenzyme Q10 increasedglutathione peroxidase activity (P <0.001) and superoxide dismutase (P <0.05) signifcantly compared withcysteamine group.Based on the results of this study, coenzyme Q10 and vitamin C can be used in reducing oxidative stress inducedcysteamine. Manuscript profile -
Open Access Article
105 - Effect of cold stress on chlorophyll content, chlorophyll fluorescence and activity of oxidative enzymes of barley cultivars (Hordeum vulgare L.)
Ali Bashirzadeh Khanlar Dayyan Abdullayev Zaman MahmudovCold is one of the most important abiotic stresses that limits crop growth and yield worldwide. In order to investigate how 20-day-old barley seedlings adapt to short-term cold stress, the present study was carried out in a factorial experiment with four replications in MoreCold is one of the most important abiotic stresses that limits crop growth and yield worldwide. In order to investigate how 20-day-old barley seedlings adapt to short-term cold stress, the present study was carried out in a factorial experiment with four replications in the seed laboratory of Karaj Seedling and Seed Breeding Research Institute. The experimental factors included barley cultivars (cold-sensitive Karon, semi-cold-tolerant Fasih, and cold-tolerant Makoi) and cold stress with three levels of 4, 8, and 22 oC (control). Temperature treatments were applied to barley seedlings for 48 hours. The results of analysis of variance showed that the interaction of cold and cultivar was significant on most of the measured traits in this study. The mean comparison also showed that the highest value of F0 was observed in Karun and Fasih cultivars at 8 oC and in Makoi cultivar at 4 oC, while in all three cultivars, Fv value and chlorophyll a content decreased with decreasing temperature. The ratio of Fm/Fv in Makoui and Fasih cultivars decreased with decreasing temperature, however no significant change was observed in Karon cultivar. In addition, with decreasing temperature, malondialdehyd (MDA) and H2O2 concentration and peroxidase (POX) activity increased in all three cultivars, especially Karon cultivar, but catalase (CAT) and super oxid dismutase (SOD) activity were not affected by the cultivar, and the highest CAT and SOD activity were obtained at 8 and 4 oC, respectively. The obtained results showed that the cultivars more tolerant than the sensitive cultivar (Karon) at low temperatures by limiting the production of compounds such as H2O2 and MDA, had higher chlorophyll content and photosynthetic efficiency, which probably reflects greater adaptation to cold. Manuscript profile
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