The effect of Nerolidol on the Superoxide Dismutase Enzyme Levels and mRNA Expression of CREB-1 Gene in Alzheimer's Model of Male Wistar Rats

Subject Areas : Journal of Animal Biology

Peyman Taheri ¹ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
Hajebrahimi Zahra ² (Aerospace Research Institute, Ministry of Science Research and Technology, Tehran, Iran)
Parichehreh Yaghmaei ³ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)
K. Parivar ⁴ (Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran)

Received: 2023-12-16 Accepted : 2025-01-30 Published : 2024-08-06

Keywords: Alzheimer's disease, Nerolidol, CREB-1 gene, Superoxide dismutase enzyme.,

Abstract :

Alzheimer's is the most important cause of neurological deterioration among elderly people, which is accompanied by the formation of amyloid plaques in the hippocampus region of the brain. One of the most important factors causing and progressing the disease is inflammation and oxidative stress. Nerolidol with antioxidant and anti-inflammatory properties is one of the secondary metabolites in some plants. CREB is one of the gene transcription factors that play a significant role in hippocampal neurons. The present study aimed to study the effect of nerolidol on the expression changes of the CREB-1 gene and superoxide dismutase enzyme in Alzheimer's model induced by beta-amyloid in male Wistar rats. The number of 48 male Wistar rats were divided into 8 groups including control, sham, Alzheimer's model, drug solvent, Alzheimer's with donepezil, Alzheimer's with nerolidol dose 50 and 100 mg/kg and protective group (treatment with nerolidol before induction of Alzheimer's). Biochemical analysis of the hippocampus, Real-Time PCR, and statistical analysis of data were performed using one-way analysis of variance and Tukey's test. Alzheimer's disease decreased CREB-1 gene expression and superoxide dismutase enzyme levels in the hippocampus. Donepezil and nerolidol, especially in the 100 dose and protective group, reduced Alzheimer's symptoms by increasing CREB-1 gene expression and superoxide dismutase enzyme levels. These findings indicate the antioxidant properties of nerolidol. Therefore, nerolidol may be effective in improving Alzheimer's disease and the damage caused by it, and it can probably be effective in preventing Alzheimer's in susceptible people with a family history of Alzheimer's.

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