Background & Aim: Increasing of drug resistances and more attention to the hazards of side effects of synthetic drugs has caused extensive researches on medicinal plant resources. The aim of this study was to determination of the chemical composition of native Silybum marianum and Foeniculum vulgare extractions in Ardabil, Iran; and investigation of their anticancer effect on liver cancer cell line HepG2 and antimicrobial effects on Staphylococcusaureus and Pseudomonas aeruginosa to propose a solution to control or treatment. Experimental: The chemical compositions of extractions performed through Soxhlet were analyzed by GC/MS system. The anticancer effect was assayed by MTT method and their antimicrobial activities were tested using disc-diffusion assay as well as MIC and MBC values estimated according to the microdilution method. Results: The analysis of S. marianum extract showed the presence of 29 compounds, mostly (2.63%) Nonane, (4.68%) Camphor, (2.84%) Borneol, (3.65%) Trans-anethole, (8.78%) Viridiflorol, (34.26%) α–Selinene, (2.34%) Longiborn-9-ene, (2.52%) amorphane-B, (5.00%) Erucylamide, (10.15%) o-Terphenyl and (46.8%) α-androst-8-ene;F. vulgare extract contained 14 compounds, mainly (5.53%) Fenchone, (5.53%) α- Thujone, (2.84%) Nonane, (79.10%) trans-Anethol and (2.51%) Hentriacontane. The effects of F. vulgare extract on Staphylococcus and S. marianum extract on Pseudomonas were remarkable. In the combined design, there was no clear effect. MIC and MBC values confirmed the results of the sensitivity test. The cytotoxic effects of extractions on liver cancer cells were the optimum in 25μg/ml after 48 hours. Recommended applications/industries: According to the results, these compounds are able to replace by chemical preservatives in food and drugs industries.
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