Investigation of Compositions and Effects of Local Herbal Silybum marianum and Foeniculum vulgare extractions on Hospital Acquired Infections (HAI) and Cell Line of liver Cancer (HepG2) by MTT assays
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
گلثوم
صمدی اندزاگی
1
(گروه بیولوژی، واحد اردبیل، دانشگاه ازاد اسلامی، اردبیل، ایران)
هاشم
یعقوبی
2
(گروه بیولوژی، واحد اردبیل، دانشگاه ازاد اسلامی، اردبیل، ایران)
مسعود
فردین
3
(گروه بیولوژی، واحد اردبیل، دانشگاه ازاد اسلامی، اردبیل، ایران)
Keywords: Staphylococcus aureus, Silybum marianum, Pseudomonas aeruginosa, HepG2, Foeniculum vulgare,
Abstract :
Background & Aim: Increasing of drug resistances and more attention to the hazards of side effects of synthetic drugs has caused extensive researches on medicinal plant resources. The aim of this study was to determination of the chemical composition of native Silybum marianum and Foeniculum vulgare extractions in Ardabil, Iran; and investigation of their anticancer effect on liver cancer cell line HepG2 and antimicrobial effects on Staphylococcusaureus and Pseudomonas aeruginosa to propose a solution to control or treatment. Experimental: The chemical compositions of extractions performed through Soxhlet were analyzed by GC/MS system. The anticancer effect was assayed by MTT method and their antimicrobial activities were tested using disc-diffusion assay as well as MIC and MBC values estimated according to the microdilution method. Results: The analysis of S. marianum extract showed the presence of 29 compounds, mostly (2.63%) Nonane, (4.68%) Camphor, (2.84%) Borneol, (3.65%) Trans-anethole, (8.78%) Viridiflorol, (34.26%) α–Selinene, (2.34%) Longiborn-9-ene, (2.52%) amorphane-B, (5.00%) Erucylamide, (10.15%) o-Terphenyl and (46.8%) α-androst-8-ene;F. vulgare extract contained 14 compounds, mainly (5.53%) Fenchone, (5.53%) α- Thujone, (2.84%) Nonane, (79.10%) trans-Anethol and (2.51%) Hentriacontane. The effects of F. vulgare extract on Staphylococcus and S. marianum extract on Pseudomonas were remarkable. In the combined design, there was no clear effect. MIC and MBC values confirmed the results of the sensitivity test. The cytotoxic effects of extractions on liver cancer cells were the optimum in 25μg/ml after 48 hours. Recommended applications/industries: According to the results, these compounds are able to replace by chemical preservatives in food and drugs industries.
Ahmad A, Alkarkhi A. F. M, Hena S, Khim L. H. Extraction, Separation and Identification of Chemical Ingredients of Elephantopus Scaber L. 2009.Using Factorial Design of Experiment. International Journal of Chemistry, 1(1): 36- 49.
Ahmad Ghareeb B. A. 2013.Assessment of Cellular Responses to Plant Extracts in Jenin Area Ranges (Ornithoganum narbonense and Silybum marianum), Problems and Solutions. Advances in Life Science and its Applications (ALSA), 1(4): 84-90.
Alsayyad J, Hamadeh R. 2007. Cancer incidence among the Bahraini population: A five-year (1998-2002 experience. Ann Saudi Med, 27(4): 251-258.
Aprotosoaie AC, Şpac A, Hăncianu M, Miron A, Tănăsescu Vf, Dorneanu V, Stănescu U. 2010. The chemical profile of essential oils obtained from fennel fruits (Foeniculum vulgare mill.). FARMACIA, 58(1): 46-53.
Baver AW, Kirby WM, shreeis JC, turck M. 1996.Antibiotic Susceptibity testing by astandardized single disc method. Am. J. Chin. Pathol, 45: 493-496.
Bosch FX, Ribes J, Diaz M and Cleries R. 2004. Primary liver cancer: Worldwide incidence and trends. Gastroenterology, 127(5): 5-16.
Clara AA, Şpac1 A, Hăncianu1m, Miron1 A, Floria TV, Dorneanu V, Stănescu U. 2010. The chemical profile of essential oils obtained from fennel fruits (foeniculum vulgare mill.). FARMACIA, 58(1): 46-53.
Etemadi A, Sadjadi A, Semnani SH, et al. 2008. Cancer Registry in Iran: A brief overview. Arch Iran Med, (5)11: 577-580
Forouzandeh F, Salimi S, Naghsh N, Zamani N, Jahani S. 2014.Evaluation of anti-cancer effect of Peganum harmala L hydroalcholic extract on human cervical carcinoma epithelial cell line (HeLa). J Shahrekord Univ Med Sci, 16(4): 1-8.
Hall AJ, Wild CP. 2003. Liver cancer in low and middle income countries. BMJ, 326 (7397): 994-995.
Hajlaoui H, Ben A. F, Snoussi M, et al. 2010. Effect of Mentha longifolia L. ssp longifolia essential oil on the morphology of four pathogenic bacteria visualized by atomic force microscopy. African Journal of Microbiology Research J, 4 (11): 1122-1127.
Hajlaoui H., Snoussi M., Ben Jannet H., et al. 2008. Comparison of chemical composition and antimicrobial activities of Mentha longifolia L. ssp. longifolia essential oil from two Tunisian localities (Gabes and Sidi Bouzid). Annals of Microbiology J. 58 (3): 513- 520.
Humphreys H, Syth ET. 2006. Prevalence surveys of healthcare-associated infections: what do they tell us, if anything? Clinical Microbiology and Infection, 12(1): p. 2-4.
Jafari A, Ghane M, Arastoo SH. 2011.Synergistic antibacterial effects of nano zinc oxide combined with silver nanocrystales. African Journal of Microbiology Research, 5(30): 5465-5473.
Juodeikiene G, Cizeikiene D, Ceskeviciute V, Vidmantiene D, Basinskiene1 L, Akuneca I, et al. 2013. Solid-State Fermentation of Silybum marianum L. Seeds Used as Additive to Increase the Nutritional Value of Wheat Bread. Food Technol. Biotechnol, 51 (4): 528–538.
Kermanshah H, Hashemi K.S, Arami S, Mirsalehian A, et al. 2011. Antibacterial effect of hydro alcoholic extract of Saliva Officinalis and Mentha longifoliaon three bacteria cause tooth decay in vitro. J Shaheed Beheshti University of Dental, 4: 232-237.
Khosravi AD, Parhizgari N, Abbasi Montazeri E, Mozaffari A, Abbasi F. 2013.The Prevalence of Bacteria Isolated From Endotracheal Tubes of Patients in Golestan Hospital, Ahvaz, Iran, and Determination of Their Antibiotic Susceptibility Patterns. Jundishapur J Microbiol, 6(1):67-71.
Kiran Kaur A, Wahi AK, kumar B, Bhandari A, Prasad N. 2011. Milk Thistle (Silybum marianum): A review. International Journal of Pharma Research and Development (ijprd), 3(2): 1-10.
Mahmoudi R., Ehsani A., Tajik H., et al. 2011. Antimicrobial effects of Mentha Longifolia L. Essential oil and Lactobacillus casei against staphylococcus aureus in Iranian White Cheese. Archive of SID3 J, 1: 147-161.
Malhotra S, Sharma S, Hans C. 2014. Prevalence of Hospital Acquired Infections in a tertiary care hospital in India. International Journal of Medicine and Medical Sciences, 1(7): 91-94.
Nordenstedt H, White DL, El-Serag HB. 2010. The changing pattern of epidemiology in hepatocellular carcinoma. Dig Liver Dis, 42(3): 206-214.
Parvin N, Validi M, Banitalebi M, Mobini GhR, Ashrafi K, Farrokhi E, Rafieian M, Akbari N, Safdari F, Rafei-Vardanjani L. 2010.Effect of medicinal smokes on some nosocomial infection factors. Shahrekord University of Medical Sciences, 12(2):76-84.
Raal A, Orav A, Arak E. 2012. Essential oil composition of Foeniculum vulgare Mill. Fruits from pharmacies in different countries. Nat Prod Res, 26(13):1173-1178.
Rahimi E, Alian F. 2013. Presence of enterotoxigenic Staphylococcus aureus in cow, camel, sheep, goat, and buffalo bulk tank milk. Veterinarski Arhiv, 83 (1): 23-30.
Rasaei S, Sadeghifard N, Zolfaghari MR, Ghafoorian S, Shakib P, Valizadeh A. 2013. Study of PAPI-1 Frequency in Clinical Strains of Pseudomonas Aeruginosa. Scientific Journal of Ilam University of Medical Sciences, 21(5):67-72
Roudbar Mohammadi Sh, Roudbari M, Vahidi M, Mohammad Hassan Z, Darabi N. 2012. Evaluation of Anti-tumor activity of Candida albicans Cell wall fraction on human Fibrosarcoma cell in vitro. J Army Univ Med Sci, 10 (2): 103-110.
Villarini M, Pagiotti R, Dominici L, Fatigoni C, Vannini S, Levorato S et al. 2014. Investigation of the Cytotoxic, Genotoxic, and Apoptosis-Inducing Effects of Estragole Isolated from Fennel (Foeniculum vulgare). J. Nat. Prod., 77 (4): 773– 778.