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Open Access Article
1 - Investigating antitumorigenic effects of Vaccinium arctostaphylos on colorectal cancer cells
Fatemeh Karami Mahsa Shahnazari Asa Ebrahimi Mahmood Khosrowchahli -
Open Access Article
2 - The correlations between chemical structure properties and antiviral activities of HIV-1 inhibitors: The study of anti-AIDS
Majid Monajjemi Anita Abedib -
Open Access Article
3 - The study of alpha-tocopherol cytotoxicity on canine mammary gland cancer cells (CF41.Mg) invitro
ریحانه سفیدابی، پژمان مرتضوی .Breast cancer is the most common cause of cancer in female dogs. Created mutations in genes associated with cancer and oxidative modification of proteins and reactive aldehydes are key events that can increase cancer risk. The role of antioxidants in prevention and trea MoreBreast cancer is the most common cause of cancer in female dogs. Created mutations in genes associated with cancer and oxidative modification of proteins and reactive aldehydes are key events that can increase cancer risk. The role of antioxidants in prevention and treatment of cancers have been reported by several studies. The aim of this study was to evaluate invitro, the antiproliferative effect of alpha-tocopherol against canine mammary gland carcinoma cell line (CF41.Mg). CF41.Mg cells were cultured in RPMI 1640 medium. The cells were treated with different concentration of alpha-tocopherol for 24, 48 and 72 h. Antiproliferative effect of alpha-tocopherol was investigated using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-tetrazolium bromide) assay. The results indicated that alpha-tocopherol inhibited proliferation of canine mammary gland carcinoma cells so that treatment with 100 microM alpha-tocopherol for 72 h resulted in a significant decrease in cell viability. The alpha-tocopherol showed less effect after 48 h. The results of this study confirm the inhibitory effect of alpha-tocopherol on canine breast cancer cells (CF41.Mg). Manuscript profile -
Open Access Article
4 - Synthesis, In vitro Antimicrobial and Cytotoxic Activities of Some Novel Bis- 1, 3, 4-oxadiazoles
Pavana Teja Pudota Raghunandan Shurpali Madhusudan Purohit G.V. Pujar -
Open Access Article
5 - Antibacterial and Cytotoxicity Effects of TiO2-Grafted Cellulose Nanocomposite
Zari Fallah Hossein Nasr Isfahani Mahmood Tajbakhsh Mojtaba Mohseni Hamed Tashakkorian Abdoliman Amouei Ebrahim Zabihi Zeinab Abedian -
Open Access Article
6 - Synthesis and In Vitro Cytotoxic Activity of Novel [1,3] Dioxolo[4,5-g]Chromen-8-ones as a Chalcone-Like Agent
Eskandar Alipour Zinatsadat Mousavi Tina Shojaie Susan Kabudanian Ardestani -
Open Access Article
7 - Phytoconstituents profiling of Cissus rotundifolia (Forssk.) Vahl. by HPLC-MS/MS, and evaluation of its free radical scavenging activity (DPPH) and cytotoxicity
Ataa Said Elsayed Ali Aboutabl Farouk Rasmy Melek Gehad Abdel Jaleel Raheem Abdel Jaleel Mona Raslan -
Open Access Article
8 - Ficusanol, a new cinnamic acid derivative and other constituents from the roots of Ficus exasperata Vahl. (Moraceae) with antioxidant and cytotoxic activities
Stevine Claudiale Popwo Tameye Jean Claude Ndom Edwige Laure Nguemfo Jean Duplex Wansi Juliette Catherine Vardamides Anatole Guy Blaise Azebaze -
Open Access Article
9 - A new phenylpropanoid glucoside from Psorospermum tenuifolium Kotschy (Hypericaceae)
Gervais Mouthe Happi Alexis Sylvain W. Mbobda Marcel Frese Simeon Fogue Kouam Jean Claude Tchouankeu Bruno Ndjakou Lenta Norbert Sewald -
Open Access Article
10 - Constituents of the ripe fruits of Nauclea latifolia Sm. (Rubiaceae) and their antileishmanial activities
Argan Kelly Nkwenti Wonkam Cyrille Armel Ngansop Njanpa Joël E.T. Ateba Yannick S.F. Fongang Angelbert Awantu J.J.K. Bankeu Jean Rodolph Chouna Fabrice F. Boyom Norbert Sewald Bruno Lenta -
Open Access Article
11 - Chemical composition and cytotoxicity of the essential oil of Tanacetum abrotanifolium (L.) Druce (Asteraceae) from Iran
Ali Sonboli Sepideh Ghilichpour Mohsen Farhadpour Mitra Arman -
Open Access Article
12 - Phytochemical study and anti-inflammatory activity of the roots of Mangifera indica L. in lipopolysaccharide (LPS)-stimulated peritoneal macrophages
Yolande Nangue Eulogio José Llorent-Martínez Maria Luisa Fernández-de Córdova Douglas Alphonse M. Ngangoum Télesphore Benoit Nguelefack Anatole Guy Blaise Azebaze Alain Bertrand Dongmo -
Open Access Article
13 - Chemical composition, herbicidal, antifeedant and cytotoxic activity of Hedychium spicatum Sm.: A Zingiberaceae herb
Avneesh Rawat Payal Thapa Om Prakash Ravendra Kumar A.K. Pant R.M. Srivastava D.S. Rawat -
Open Access Article
14 - Study of the antileukemic activity of Mimosa caesalpiniifolia Benth. ethanolic extract and fractions
Gabriele Taumaturgo Mororó José Roberto de Oliveira Ferreira Michel Mualén de Morais Alves Nayana Bruna Nery Monção Laís Campos Teixeira de Carvalho-Gonçalves Antônia Maria das Graças Lopes Citó Paulo Michel Pinheiro Ferreira Fernando Aécio de Amorim Carvalho Juan Carlos Ramos Gonçalves -
Open Access Article
15 - Evaluation Of Cytotoxic Effect Of Aqueous andHydroalcoholic Extracts Of Guava Fruit Psidium guajava L on Cell Line DU-145
Sholeh Zaeemi Baravati کهین شاهانی پور Ramesh MonajemiInroduction and Objective:Prostate cancer is the second most common cancer in men after skin cancer. Guava Fruit with scientific name Psidium guajava L. are considered as an important source of phenolic compounds and antioxidant effect.In this study, the cytotoxic effec MoreInroduction and Objective:Prostate cancer is the second most common cancer in men after skin cancer. Guava Fruit with scientific name Psidium guajava L. are considered as an important source of phenolic compounds and antioxidant effect.In this study, the cytotoxic effect of aqueous and hydroalcoholic extracts of Guava fruit and effect of the extracts on DU-145 cell line was and its effect on prostate cancer cells was studied. Materials and Methods:Guava fruit was collected from Sistan and Baluchestan region. The aqueous and hydroalcoholic extracts were prepared from this plant. Class of DU-145 cells was cultured in RPMI-1640 medium containing 10% bovine serum in a 5% CO2 incubator, and under different concentrations of aqueous extract of 24, 48 and 72 hours were incubated. MTT assay for cell survival in the presence and absence of extract was used to calculate the percentage and absorbance at 540 nm was measured by ELISA. Data were assessed using SPSS software(* = P-value Results:The results showed that the aqueous and alcoholic extract Guava fruit is a cytotoxic effect. Further studies showed that the cytotoxic effect of aqueous extracts of the ethanol extract is much stronger. Manuscript profile -
Open Access Article
16 - Study Of Cytotoxisity Effects Of Aqueous Extract Of Physalis. alkekengi Against U937 Cell Line
Parvin Torabzadeh Mahroz DezfulianIntroduction and Objective:Physalis-alkekengiof Solanaceace has been recommended as a traditional antipyretic, antineoplastic, anti inflammation, analgestic, anti bacterial, anti virus and anti tumor drug by herbalist in Iran. For this purpose we decided to study cytoto MoreIntroduction and Objective:Physalis-alkekengiof Solanaceace has been recommended as a traditional antipyretic, antineoplastic, anti inflammation, analgestic, anti bacterial, anti virus and anti tumor drug by herbalist in Iran. For this purpose we decided to study cytotoxic effects of the aqueous extract of it on the U937 Cell.Materials and Methods:At first, the aqueous extracts of the plant were obtained. Then three different doses of this extract (low, medium and high) were effected on U937, Hela and L929 cell cultures, and by MTT Assay viability of this cells were evaluated.Results:The results show that significant difference was observed in surviving of U937, Hela and L929 cells, when control group and treated group was compared.The LC50 for the U937 cells was28.125 mg/ml.ConclusionThe results discovered that aqueous extracts of the Physalis-alkekengi have good cytotoxic effects on U937 cells. Manuscript profile -
Open Access Article
17 - Evaluation Of the Effects Of Different Concentrations Of Aloe veraExtract on the Proliferation Of Uterus Cervical Cancer Cells(Hela)Of Humans
Elahe Ebrahimi Masoud Parsania Hamid Hosseini dostInroduction and Objective: Cervical cancer is the second most common cancer in womenHigh incidence ofcerviccancer and the side effects of the treatments with synthetic chemical compounds demonstrated that hazards are immense.Considering the side effects, treatment with MoreInroduction and Objective: Cervical cancer is the second most common cancer in womenHigh incidence ofcerviccancer and the side effects of the treatments with synthetic chemical compounds demonstrated that hazards are immense.Considering the side effects, treatment with extracts of plants such as Aloe vera have proved to be reducing the levels of side effects and can bemuch cheaper alternative. So, anti cancer properties of the extracts of Aloe vera against the Hela cells of humanwas evaluated in tissue culture, in this study.Material and Methods:After gatheringand confirmthe desiredplant species.Extractionwas performed bymacerationThe toxicity effects of different concentrations 16,17,18,19, 20 and 21 mg/ml of extract of Aloe veraon the Hela cells of human, were evaluated using Trypan blue and MTT methods,on 24, 48 and 72 hours after exposure to a mediumcontaining 1serum and extractResults: 20 mg/ml concentration ofAloe veraextract was toxical effects on Hela cells of human based on the results obtained, it was concluded that extracts of Aloe verainhibit the proliferation of Hela cells of human Manuscript profile -
Open Access Article
18 - Fe-Co-V/Zeolite nano composite catalyzed biginelli compounds synthesis and evaluation of their drug cytotoxic activity
shima Zanganeh Yousef Abadi Mohammad Kazem Mohammadi Haman Tavakkoli -
Open Access Article
19 - Chemical composition, anti-fungal properties, anti-oxidant activity and cytotoxicity of Thymus daenensis Celak. essential oil
abdelnasser mohammadi kholami Sima Shiravand Katrin EbrahimiThyme daenensis, scientifically known as Thymus daenensis Celak belongs to the Lamiaceae family. The aerial part of Thymus daenensis encompasses phenolic compounds and has powerful antifungal and antioxidant properties. Due to its potential to produce the secondary meta MoreThyme daenensis, scientifically known as Thymus daenensis Celak belongs to the Lamiaceae family. The aerial part of Thymus daenensis encompasses phenolic compounds and has powerful antifungal and antioxidant properties. Due to its potential to produce the secondary metabolites, it also has anti-mutation and anti-cancer properties. In this study, in order to investigate the phytochemicals, antioxidant, antifungal properties and also the cell cytotoxicity, the aerial parts of Thymus daenensis were collected from Zagheh area in Lorestan province Khorramabad, in June 2017. The chemical compounds of the essential oil were extracted using water distillation and analyzed using Chromatography (GC) and Gass Chromatography-Mass Spectrometry (GC/MS). Broth microdilution, MTT, and DPPH methods were utilized to determine the antifungal and antioxidant properties and cell cytotoxicity, respectively. In the essential oil, the most significant compounds were thymol (24.7%) and carvacrol (38.5%) and the Minimum inhibitory concentration against Aspergillus fumigatus and Aspergillus niger were respectively 0.5 and 1 μg/ml. The radical inhibitory effect increased as the density of the essential oil increased (IC50=63/23). The MTT assay indicated that the impact of the essential oil cytotoxicity on human lymphocyte cells was insignificant. According to the results obtained, it can be concluded that the essential oil of Thymus daenensis, as one of Iran’s indigenous plants, has considerable antioxidant properties that have potential to be used in pharmaceutical industrieTs and act as a good candidate in the fungal diseases’ treatment. Manuscript profile -
Open Access Article
20 - Evaluation of phytochemical, cytotoxic, antioxidant and antibacterial activity of of Melissa officinalis L. from Marivan region
Morteza Yazdani fereshteh jookar kashi Zeinab Toluei Akram Rahimi-MoghaddamConsidering the adverse effects of synthetic antioxidants, antibiotics, and anti-cancer drugs on human health and increasing the antibiotic resistance of pathogens, it is a necessity to find safe alternative sources for these compounds. For this purpose, Melissa officin MoreConsidering the adverse effects of synthetic antioxidants, antibiotics, and anti-cancer drugs on human health and increasing the antibiotic resistance of pathogens, it is a necessity to find safe alternative sources for these compounds. For this purpose, Melissa officinalis L. was collected from Marivan, Kurdestan province, and its chemical compositions and bioactivities were determined. Aerial parts of self-sowing M. officinalis were harvested during the flowering stage at 1320 meters above the sea level in 2016 summer. Using solvent-free microwave extraction methodessential oil of M. Officinalis was obtained and their component was identified by gas chromatography/mass spectrometry. The antioxidant and cytotoxicity activity of the essential oil were determined via β–carotene bleaching assay and brine shrimp lethality test, respectively. The antibacterial activity of the essential oil was evaluated by agar well diffusion method and minimum inhibitory concentrationdetermination against various types of standard and clinical microbial strains.Extraction yield of the essential oil was 1.18%, and the main componentswere geraniol (30.39%) and geranial (26.19%).Based on the results of β–carotene bleaching assay, inhibition of linoleic acid oxidation by the essential oil and BHT were 72.48% and 96.48%, respectively.Using the brine shrimp lethality test, LC50 of theessential oil and vincristine sulfate were obtained 62.76 μg/ml and 0.751 μg/ml, respectively.Thus, this study revealed that essential oil of M. officinalis had good antimicrobial activity against testedmicroorganisms, especially Gram-positive bacteria.In addition, it showed significant antioxidant, antibacterial and anticancer activities, so it seems that essential oil of M. officinalis may have potential use as an alternative to synthetic antioxidants, antibiotics and anticancer drugs. Manuscript profile -
Open Access Article
21 - Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in North Khorasan province
Ali Koohestanian Maryam Taghizadeh Maliheh Samadi Kazemi Ahmad Asgharzadeh Seyedeh Faezeh TaghizadehThe aim of this study was to Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in a complete randomized design with three replications. R. persica L. was collected from Shirvan city located in North Khorasan province in the late summer of MoreThe aim of this study was to Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in a complete randomized design with three replications. R. persica L. was collected from Shirvan city located in North Khorasan province in the late summer of 2019 . The methanol, petroleum ether, ethyl acetate and water extracts were prepared by maceration method and were analyzed by using the liquid chromatography-mass spectrometer system, it was determined that the methanolic extract had the highest amounts of rosmarinic acid compounds. Antimicrobial effects of different extracts were investigated by determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Antimicrobial assays against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhi, Serratia marcescens, Escherichia coli, Candida albicans, Aspergillus niger showed that in all treatments, gram-negative bacteria were more resistance in compare to gram positive bacteria. In all extracts, the most sensitive strain was S. aureus with MIC and MBC of 10.89±0.13 and 19.34±0.33 μg/ml in the methanolic extract, respectively. Methanolic extracts of aerial parts had significantly stronger antimicrobial effects, respectively. Based on the results of cytotoxicity of different extracts on MCF-7, DU-145, PC3, A2780, C26, U-87-MG, MDA-MB-231, and Hela, it was found that Methanolic extract had the most cytotoxic effects on all cell lines studied. The lowest effects of cytotoxicity were related to the aqueous extract of aerial parts. In all treatments and cell lines, the IC50 was less than 400 μg/ml. The biochemical and biological activities of R. persia may be related to its phenolic compounds, so further studies is recommended. Manuscript profile -
Open Access Article
22 - Histopathological study on acute toxicity of nanochelating based silver nanoparticles in mouse model
Seyedeh Mahsan Hoseinialfatemi Abdollah Karimi Fatemeh Fallah Shahnaz Armin Maryam Hafizi Somaye Kalanaki -
Open Access Article
23 - Histopathological study on acute toxicity of nanochelating based silver nanoparticles in mouse model
Seyedeh Mahsan Hoseini-Alfatemi Fatemeh Fallah Abdollah Karimi Maryam Hafizi Shahnaz Armin Somaye Kalanaki -
Open Access Article
24 - فعالیت سیتوژنتیک عصاره متانولی اندامهای هوایی گیاه Plumbago europaea بر روی سلولهای مغز استخوان Balb/C
احمد خلیل حنان بشاره احمد ال-اوکلاهBackground & Aim: The World Health Organization (WHO) estimated that majority of the inhabitants of the world rely chiefly on folk medicine. It, therefore, approved the use of herbal products for national policies and drug regulatory measures in order to strengthen MoreBackground & Aim: The World Health Organization (WHO) estimated that majority of the inhabitants of the world rely chiefly on folk medicine. It, therefore, approved the use of herbal products for national policies and drug regulatory measures in order to strengthen research and evaluation of the safety and efficacy of herbal products. We have evaluated the cytotoxic, mutagenic and antimutagenic potential of the methanolic extract of the aerial parts of P. europaea, a common species in the Mediterranean and Central Asia. Experimental: Male Balb/C mice were intraperitoneally (i. v.) injected with varying doses of the extract dissolved in dimethyl sulfoxide (DMSO). The i.v. LD50 of the extract was determined to be 58.33mg/kg body weight. Bone marrow cells were processed and screened for chromosomal aberration and micronucleus formation. Mitomycin C (MMC, 2mg/kg b. w.) and DMSO (0.5%) served as positive and negative control, respectively. Antimutagenecity was followed by administration of 2mg MMC/kg in the beginning of the first or the last 24h of applying 14.6mg/kg of the extract daily for 7 consecutive days. Results: The selected doses of the extract elevated mitotic index and increased percentage of aberrant cells compared to the negative control. However, at a dose of 14.6mg/kg, the extract was enough to reduce significantly the toxic effects induced by MMC. This indicated that the P.europaea extract by itself is mutagenic, but antimutatgenic probably at small doses and can modulate the mutagenicity of MMC. Recommended applications/ industries: These data may help in understanding of therapeutic properties of P. europaea claimed by folk medicine. However, caution regarding indiscriminate use of this plant by the public is necessary. Multiple experiences are needed to rule out any possible side effects and to prove health safety of this natural product before introducing it into the market for therapeutic purposes. Manuscript profile -
Open Access Article
25 - تجزیه و تحلیل مقایسه ای GC-MS، فعالیت های آنتی اکسیدانی و سیتوتوکسیک دانه و عصاره n-هگزانی پوست ساقه Garcinia kola Heckel
سیده آکورو اوینلاد اوگوندار آدکونله اویدولاBackground & Aim: Garcinia kola is one of the well-known tropical medicinal plants known in the traditional management of several diseases especially connected with inflammatory and degenerative diseases. This study was designed to compare the phytochemical contents MoreBackground & Aim: Garcinia kola is one of the well-known tropical medicinal plants known in the traditional management of several diseases especially connected with inflammatory and degenerative diseases. This study was designed to compare the phytochemical contents and cytotoxic activities of crude G. kola n-hexane seed and stem-bark extracts by Gas Chromatography-Mass Spectrometry (GC-MS).Experimental: Fresh seeds and stem-bark of G. kola were collected, authenticated and extracted by macerating in n-hexane separately. The compounds in the crude extracts were evaluated using Gas Chromatography-Mass Spectrophotometer (GC-MS). The cytotoxic activity was determined using the brine shrimp lethality assay.Results: Extraction of the plant materials yielded the crude n-hexane extracts GKSE and GKBE from the G. kola seed and stem-bark, respectively. GC-MS analysis detected 54 and 34 compounds in GKSE and GKBE, respectively. The most abundant compounds in both extracts were Lanosta-8,24-dien-3-one and Oxacycloheptadec-8-en-2-one with percentage abundance of 20.72% and 22.71%, respectively. The stem-back extract (LC50=42.76±1.85 μg/mL) showed better cytotoxic activity than the seed extract (LC50=73.15±1.51 μg/mL). GC-MS results for both extracts indicated compounds known for their antioxidant and anticancer properties which may explain some of the various ethno-medical uses of G. kola seed and stem-bark.Recommended applications/industries: This plant could be a source of a new anticancer drug. Manuscript profile -
Open Access Article
26 - Vicia variabilis a Nutritive Feed for Domesticated Animals: A Survey about Cytotoxic and Antioxidant Activity
M. Mosaddegh M. Hamzeloo Moghadam F. Naghibi S. Mohebby A. Pirani B. Eslami -
Open Access Article
27 - Toxicity of pesticides to plants and non-target organism: A comprehensive review
Aisha Kamal Farhan Ahmad Mohd Shafeeque -
Open Access Article
28 - ارزیابی فعالیت های ضد میکروبی و سمیت سلولی عصاره های متانولی برگ گیاه Sansevieria zeylanica (L.) Willd. روی سرطان سینه در انسان، لاین های سلولی MDA-MB-231
S. Rajashekara S. Chudamani S. Rautela Sharvani Hegde S. Swaroopaگیاه Sansevieria zeylanica یک گونه علفی چند ساله مناطق خشک است که در قسمت­های گرمسیر و نیمه گرمسیر دنیا پراکندگی دارد. این گیاه، یک گیاه شگفت­انگیز دارویی است و مزایای زیادی دارد. هدف این مطالعه تعیین فعالیت­های سمیت سلولی در سرطان سینه در انسان روی لاین­ Moreگیاه Sansevieria zeylanica یک گونه علفی چند ساله مناطق خشک است که در قسمت­های گرمسیر و نیمه گرمسیر دنیا پراکندگی دارد. این گیاه، یک گیاه شگفت­انگیز دارویی است و مزایای زیادی دارد. هدف این مطالعه تعیین فعالیت­های سمیت سلولی در سرطان سینه در انسان روی لاین­های سلولی MDA-MB-231 و نیز ارزیابی خاصیت ضد میکروبی با استفاده از عصاره برگ متانولی گیاه S. zeylanica است. آزمایش اصلی تعیین حساسیت میکروب­ها در برابر عصاره­های متانولی برگ گیاه S. zeylanica است. درصد بازدارندگی رشد اندازه­گیری شد و غلظت نمونه برای ممانعت از رشد سلول تا 50 درصد (IC50) برای درصد زنده­مانی تعیین شد. عصاره­های برگی بدست آمده از گیاه S. zeylanica روی لاین­های سلولیMDA-MB-231 سرطان سینه در انسان اثر سمیت سلولی نشان دادند. شاخص IC50 (78/1167 میکروگرم در میلی­لیتر) پس از تیمار 24 ساعته روی این لاین­ها بدست آمد. افزایش مرگ سلولی روی لاین­های MDA-MB-231 با افزایش غلظت عصاره نشان می­دهد که عصاره متانولی برگ این گیاه فعال است. برای اولین بار، ما تلاش کردیم تا خصوصیات بیولوژیکی عصاره برگ متانولی گیاه S. zeylanica را ارزیابی کنیم. نتایج حاصل کاربرد درمانی را تایید کرد و می­تواند برای کاربرد وسیع به­عنوان ماده­ای ضد میکروب، مدرک معتبری باشد. در این مطالعات نتایج غربال­گری فعالیت­های فایتوشیمیایی، ضد میکروبی و سمیت گیاه S. zeylanica تایید کرد که این گیاه می­تواند به­عنوان منبع گیاهی طبیعی مورد استفاده قرار گیرد. این گیاه می­تواند برای مصارف دارویی و داروشناسی در مقیاس وسیع و بصورت صنعتی استفاده شود. Manuscript profile -
Open Access Article
29 - Evaluation of cytotoxic effect of Ruta graveolens L. Hydro alcoholic extract in human cervical cancer cells (HeLa)
seyed milad mousavi jazayeri elahe ebrahimi Masoud parsaniaAbstract Introduction Cervical cancer is one of the most common cancers in women. In some cases, the lack of appropriate response to treatment with chemical drugs and the side effects of using synthetic drugs show necessary to achieve a natural drug that has minimal si MoreAbstract Introduction Cervical cancer is one of the most common cancers in women. In some cases, the lack of appropriate response to treatment with chemical drugs and the side effects of using synthetic drugs show necessary to achieve a natural drug that has minimal side effects. This study was evaluated effects of Hydroalcoholic extracts Ruta graveolens L. against of HeLa cell. Material and Methods HeLa cells were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum. Preparation of extract were done by maceration technique. This study were assessed the effects of different concentrations (250, 500, 750, 1000 and 1500 μg/ml) of Ruta graveolens L. on the proliferation of HeLa cells at 24, 48 and 72 hours after treatment of cells with extract and medium containing 1% serum by Trypan blue and MTT methods. Finding The cytotoxic concentration 50% (CC50) of the extract was determined on HeLa cell 1500 ug/ml. The results showed that concentrations of 1000 ug/ml of Ruta graveolens L. extract, after 72 hours showed a significant decrease in the proliferation of HeLa cells compared with control. Conclusion: The results of this study showed Ruta graveolens L. extract can inhibit the proliferation of HeLa cells. Keyword: Cytotoxic effect, Ruta graveolens L. extract, HeLa cells. Manuscript profile -
Open Access Article
30 - Comparison of Ginger(Zingiber officinale) Hydroalcoholic Extract on the Viability of Cancer Cells and Embryonic Fibroblast Cells
Elham Hoveizi Tayebeh MohammadiLung cancer is the most common cause of cancer related death. Ginger is an edible and medicinal plant having important health benefits including anticancer activity. The aim of this study was evaluation and comparison of the effect of hydroalcoholic extract of ginger on MoreLung cancer is the most common cause of cancer related death. Ginger is an edible and medicinal plant having important health benefits including anticancer activity. The aim of this study was evaluation and comparison of the effect of hydroalcoholic extract of ginger on lung cancer cells and normal mouse embryonic fibroblasts. Human lung cancer cell line A459 and normal mouse embryonic fibroblasts were cultured in vitro and treated with different concentrations of ginger extract for 24, 48 and 72 hours. The MTT assay was used to determine cell viability. Ginger extract in concentrations of 1800 and 2000 µg/ml had killed both of cells at 72 hours after treatment and caused morphological changes in cells which were more obviously in cancer cells. Ginger extract in concentrations of 1000, 1200, 1400, 1800 and 2000 µg/ml killed cancer cells more than embryonic cells(P<0.05). Cytotoxicity effect of ginger on lung cancer cells was more than its effect on normal mouse embryonic fibroblasts and can be regarded as a safe anticancer medicine. Manuscript profile -
Open Access Article
31 - Effect of aquatic garlic (Allium sativum L.) extract on the survival of breast cancer cells (MCF-7) and non-cancerous cells of mouse fibroblasts (L929)
Zahra Hassanzadeh Elmira Mikaeili Agah Asadollah Asadi Kobra Bagheri valmiGarlic has been known worldwide as a dietary constituent with many pharmacological effects in humans for centuries. The aim of this study was to compare the cytotoxicity effect of the aqueous extract of garlic on breast cancer cells (MCF-7) and non-cancerous normal cell MoreGarlic has been known worldwide as a dietary constituent with many pharmacological effects in humans for centuries. The aim of this study was to compare the cytotoxicity effect of the aqueous extract of garlic on breast cancer cells (MCF-7) and non-cancerous normal cells (L929). After culture and treatment of cells with different concentrations of extract for 24, 48 and 72 hours, the cell morphology was investigated by invert microscopy and the toxic effect of the extract was determined by MTT (3- 4, 5 dimethylthiazole 2-yl 5,2-diphenyl tetrazolium). Microscopic observations showed no change in both cell lines within 24 hours. These changes appeared after 48 and 72 hours in cancerous cells and increased after 72 hours, but no significant changes were observed in healthy cells. At the same time, the results of MTT showed that the mortality rate was significant at high doses at 24 and 48 hours (P <0.001), and in 72 hours it was very intense and survival rate reached 48% at the highest dose. However, at 24 and 48 hours, we did not see significant death in non-cancerous cells, but at higher doses of 4000 μg/ml in 72 hours, death increased significantly (P <0.001) but did not exceed 19%. Therefore, aqueous extract of garlic has a greater effect on breast cancer cells than normal cells, and can be used to prevent cancer or as a supplement to chemotherapy in the future. Manuscript profile -
Open Access Article
32 - Synthesis and characterization of silver (I) complex containing 4′-(4-quinoline)-2,2′:6′,2′′-terpyridine: Thermal behavior, luminescence study, and cytotoxic properties
Badri Zaman Momeni Sanaz Kazemzade Anari Zahra ShahsavariThe reaction of AgNO3 with 4′-(4-quinoline)-2,2′:6′,2′′-terpyridine (qtpy) has been resulted in the formation of new silver (I) complex [Ag(qtpy)(NO3)] (1). The product has been characterized by elemental analysis, infrared Fourier transmis MoreThe reaction of AgNO3 with 4′-(4-quinoline)-2,2′:6′,2′′-terpyridine (qtpy) has been resulted in the formation of new silver (I) complex [Ag(qtpy)(NO3)] (1). The product has been characterized by elemental analysis, infrared Fourier transmission spectroscopy (FTIR), and nuclear magnetic resonance spectroscopy (1H and 13C NMR). The emission spectrum of complex 1 reveals the presence of π→π* transition with a red shift relative to the free ligand due to the coordination of the ligand to silver center. The thermal properties of 1 indicate that the thermal decomposition process occurs in three steps whereas it is stable up to 320 °C. In addition, the cytotoxic effects of 1 against U87-MG human glioblastoma, MCF-7 human breast cancer, SCOV-3 human ovarian cancer cell line, HT-29 human colorectal cancer, and AGO1522 human normal skin fibroblast cell line were carried out using the MTT assay. The anti-cancer activity of 1 against U87-MG human glioblastoma cell line with IC50 of 6.93 μM is more than that of paclitaxel with IC50 of 27.38 μM. Manuscript profile -
Open Access Article
33 - Anticancer activity of vanadium complexes with bipyridine and phenanthroline
طاهره نشاطی افخم مرضیه زارعی دهنوی آنیتا عابدیVanadium is a nonessential mineral of the daily diet that has shown beneficial health effects. In the present research, we synthesized and characterized three vanadium complexes,]VOCl)bpy(2]Cl )1(, ]VOCl)phen(2]Cl )2(, and ]VO)SO4()phen(2] )3(, from the reaction of VCl3 MoreVanadium is a nonessential mineral of the daily diet that has shown beneficial health effects. In the present research, we synthesized and characterized three vanadium complexes,]VOCl)bpy(2]Cl )1(, ]VOCl)phen(2]Cl )2(, and ]VO)SO4()phen(2] )3(, from the reaction of VCl3 and VOSO4 salts with 2,2'-bipyridine)bpy( and 1,10-phenanthroline )phen( ligands. The mentioned complexes were characterized by FT-IR, Mass and UV-Vis spectroscopy. In absorption spectrum of the synthesized compounds, there is a broad weak peak, above 700 nm which is referred to d-d transition of vanadium )IV( center. Moreover, stretching vibration of V=O is appeared around 973-978 cm-1, in IR spectrum. These results confirm the structure of the compounds. Cytotoxic properties of the complexes were checked out on a normal cell NIH3T3 (Rat embryo fibroblast cell) and against three cancer cell lines including HT29 (colon carcinoma), CacoII )colorectal adenocarcinoma(, and T47D )breast cancer cell( by MTT assay and compared with cis-platin. The results indicate compound )1( with low toxicity on normal cells and high cytotoxicity on the cancerous cell lines, is capable of selectively killing properties Manuscript profile -
Open Access Article
34 - The cytotoxicity of culture extracts of indigenous Actinobacteria of Iran using Artemia urmiana
Sanaz Imanian Ali Mehrvar Javad Hamedi Hossein Samadi Kafil Naser Eivazian KariBackground & Objectives: Actinobacteria are very important in the world in terms of the production of metabolites with various biological effects. This study was conducted with the aim of investigating the toxicity of the fermented extract obtained from Actinobacter MoreBackground & Objectives: Actinobacteria are very important in the world in terms of the production of metabolites with various biological effects. This study was conducted with the aim of investigating the toxicity of the fermented extract obtained from Actinobacteria isolated from some biological resources of Iran on Artemia urmiana. These isolates were deposited in University of Tehran Microorganisms Collection (UTMC).Materials & Methods: Forty-eight -76°C actinobacterial isolates were revived using ISP2 agar culture medium. Two bacterial discs were inoculated in ISP2 broth medium as a pre-cultivation medium. After 48 hours, 10 ml of the liquid was inoculated into the fermentation culture medium and heated for 7 days in a shaker incubator with 180 rpm, temperature of 28°C and pH of 7.2 ± 0.2. The fermentation broth was extracted using ethyl acetate, the solvent was evaporated using a low-pressure rotary evaporator. The toxicity of fermentation broth extracts was assayed against 40-hour Artemia urmiana nauplii.Results: According to the toxicity classification, among the all 48 isolates, 64.58% were highly toxic, 22.91% were moderate toxic, and 12.5% were in the low toxicity compounds group.Conclusion: The results showed that more than half of the tested extracts had very high lethality on Artemia urmiana in a short time. Comparing the LC50 and LT50 values of the extracts with similar studies, it was found that these extracts have a significant biological activity and can be used as a rich source in the production of metabolites with proper biological effects. Manuscript profile -
Open Access Article
35 - Influence of Arsenic (III), Cadmium (II), Chromium (VI), Mercury (II), and Lead (II) Ions on Human Triple Negative Breast Cancer (HCC1806) Cell Cytotoxicity and Cell Viability
Tsdale F. Mehari Checo J. Rorie Sherette S. Godfrey Radiah C. Minor Sayo O. Fakayode -
Open Access Article
36 - Anti-proliferative and Cytotoxic Effects of Curcumin in MCF-7 Human Breast Cancer Cells
Hossein Abbaspour Akbar Safipour Afshar -
Open Access Article
37 - Isolation of Cerebroside from Gynura procumbens Leaves and Biological Activities of the Leaves Extracts
Md. Saddam Hossain Md. Maniruzzaman Md. Minzanur Chowdhuary Junaid Ahmed Md. Mizanur Badal Mohammad Yousuf -
Open Access Article
38 - Cellular Toxicity of Multi-walled Carbon Nanotubes on Human Lung Cells
Nafiseh Nasirzadeh Yahya Rasoulzadeh Mansour Rezazadeh Azari Yousef Mohammadian -
Open Access Article
39 - The Study of Cytotoxicity of an Imidazolium Based Ionic Liquid on MCF-7 Cell Line
Elham Sajadiyeh Masoumeh Tabatabaee SayedMorteza Seifati Zohreh Derikvend -
Open Access Article
40 - Acute Toxicity, Brine Shrimp Lethality and Phytochemical Screening of Lannea schimperi and Searsia longipes
Musa Chacha Nicolaus Mbugi -
Open Access Article
41 - Synthesis and Cytotoxic Evaluation of 2-Pyrazoline Derivative on Leukemia Cancer Cell Line K562
Masoud Shaabanzadeh Maryam Bikhof Torbati -
Open Access Article
42 - Cytotoxicity Effects of Citrus Skin Extract On Cancer Cell Line MCF-7
عباسعلی دهپور جویباری زهرا حسن پورNowadays, cancer is knownas the main cause of death throughout the word. In recent decades, medical and molecular advances have not only been able to find the reasons and mechanisms of cancer, but they were more successful in early diagnosis and treatment of it. Citrus MoreNowadays, cancer is knownas the main cause of death throughout the word. In recent decades, medical and molecular advances have not only been able to find the reasons and mechanisms of cancer, but they were more successful in early diagnosis and treatment of it. Citrus skin is the accumulation center of many antioxidant and enzymes. Flavonoids and limonene are among the important compounds in the citrus skin and their vital rolesin prevention and treatment of cancer is under study these days. In this research, after cultivation and reproduction of MCF-7cancer cells line and in order to determine, the effectof cellular cytotoxicity of citrus skin extract, these cells were put in exposure to different doses of etanolic extract and after 72 hours, MTT test was done. Results indicate that the ethanolic extract of Orange skin at concentrations 7.5,10 (p ≤ 0.05)and the ethanolic extract of Limon skin at concentrations 2.5,5 (p ≤ 0.01)decrease cell growth significantly in comparition with the control group. the effectof cellular cytotoxicity grows with an increase in the concentrationof extract and the highest percentage of growth inhibition in extract of Orange skin and Limon skin is related to the concentrations of 7.5 and 5 respectively. Thus citrus skin extract is suggested as an effective material in curing cancer in medical advances. Manuscript profile -
Open Access Article
43 - Assessment of the Cytotoxicity Activity of Elatostema umbellatum and Pileamicropylla using the Brine Shrimp Lethality Assay
امیر مدرسی چهاردهی لیلا موسوی داراه ابراهیم شیدا فریضا سلیمان عظیم هدایت پورElatostema umbellatum and Pilea microphyllaare two different genuses from the nettle family, Urticaceae which are found in the tropical climate of Southeast Asia, especially in Malaysia. The cytotoxic effects of these two species were investigated by the brine shrimp le MoreElatostema umbellatum and Pilea microphyllaare two different genuses from the nettle family, Urticaceae which are found in the tropical climate of Southeast Asia, especially in Malaysia. The cytotoxic effects of these two species were investigated by the brine shrimp lethality assay. The main purpose of this study was to evaluate the cytotoxic effects of these plants which naturally grow wild in Malaysia and has not been focused on cytotoxicity activity.Brine shrimp lethality assay was selected due to determine the cytotoxic activity against larvae of Artemiasalina.Two out of sixteen extracts namely CEPM II (chloroform extract of P. microphylla from extraction method II) and MEEU II (methanol extract of E. umbellatum from extraction method II) showed low LC50 values at 46.70µg/mL and 57.62 µg/mL, respectively. However, HEPM I (hexane extract of P. microphylla from extraction method I) showed the highest LC50 value (5742 µg/mL). Also it can be concluded that the extraction method which using separatory funnel performance was much better than the first method of extraction using only Soxhlet.Generally, crude extracts from E. umbellatum were showed higher cytotoxicity activity. Although both plants can be used as the potent cytotoxic plants for more specific tests such as anticancer activity. Manuscript profile -
Open Access Article
44 - Cytotoxic Effects of Myrtus communis Extract on Hela and MCF7 Cancer Cells
سحر حبیب زاده خامنه فرخنده نعمتی عبدالحسین شیرویThis study aimed to investigate the effects of Myrtus communis extract in 10, 7.5, 5, 2.5, 1.25, 0.625, 0.312, 0.156 mg/ml concentration on Hela and MCF7 cell lines after 72 h using the MTT assay. Hela and MCF7 cells were cultured in RPMI1640 medium containing fetal bov MoreThis study aimed to investigate the effects of Myrtus communis extract in 10, 7.5, 5, 2.5, 1.25, 0.625, 0.312, 0.156 mg/ml concentration on Hela and MCF7 cell lines after 72 h using the MTT assay. Hela and MCF7 cells were cultured in RPMI1640 medium containing fetal bovine serum, penicillin and streptomycin and incubated at 37 anddeg; C atmosphere containing 5% CO2 and 95% humidity in sterile flasks. Results show that ethanolic extract of M. communis in 1.25 mg/ml (p andle; 0.05), and 0.312, 0.156 (p andle; 0.02) concentration, was significantly reduced Hela cell growth compared to control, after 72 h. The highest percentage of growth inhibition at 0.625 mg/ml concentration was % 82.33. For MCF7 cells, ethanolic extract of M. communis in 2.5, 1.25, 0/312 mg/ml (pandle; 0/02) and 0.625 (pandle; 0/001) concentration was significantly reduced MCF7 cell growth compared to control, after 72 h. The highest percentage of growth inhibition at 1.25 mg/ml concentration was 70.64 %. The results suggest that ethanolic extract of M. communis has inhibitory effects on cell growth both on Hela and MCF7 cell line. Manuscript profile -
Open Access Article
45 - Cytotoxicity Effects of the Ferula gummosa Extract on the Cancer Cell Line MCF7
بهمن اسلامی جدیدی عباسعلی دهپوری فرخنده نعمتی بشری رضاییBreast cancer is the second most pervasive cancer among women. Breast cancer is a worldwide epidemiological problem which the current remedies cannot heal it effectively and has unwanted side effects. Therefore, attempt to produce more effective medicine with less toxic MoreBreast cancer is the second most pervasive cancer among women. Breast cancer is a worldwide epidemiological problem which the current remedies cannot heal it effectively and has unwanted side effects. Therefore, attempt to produce more effective medicine with less toxicity is essential. Ferula gummosa is anti-cancer, anti-spasm, anti-paroxysm, antiseptic and laxative and it is tonic for stomach, reconstructs surface scars, and causes more milk producing in breasts. Since, its anti-tumorous effects have not been studied on the breast cells; the cellular toxicity effect of its ethanolic extract was investigated in this study. To investigate the preventing effects of F. gummosa ethanolic extract on cellular growth, we have used the MCF7 (Human Caucasian breast adenocarcinoma) cell line which has been cultivated in RPMI environment containing cowand#39;s embryo serum and antibiotic. Then we treated these cells in different concentrations of F. gummosa ethanolic extract (0.165, 0.312, 0.625, 1.25, 2.5, 5, 7.5, and 10 mg/ml) for 72 hours. The amount of cell viability was determined by MTT method. Ethanol extract of F. gummosa has a significant cellular toxicity effect on the MCF7 cell line in 0.625, 1.25, 2.5, 5, 7.5, and 10 mg/ml densities after 72 hours compare with control group, and the calculated IC50 for F. gummosa extract was 1.765 mg/ml. The results suggest that ethanol extract of F. gummosa has preventing effect on the cellular growth of MCF7 cellular class. Manuscript profile -
Open Access Article
46 - Cytotoxicity Effects of Bishops Flower (Ammi majus) Extract on the Cancer Cell Lines Hela and MCF-7
فرخنده نعمتی بهمن اسلامی جدیدی مهدیه طالبی دارابیTodaymany of the treatment methods are used to treat cancer. Unfortunately, in most cases very poor response is often associated with undesirable side effects. AlphaLack ofresponse to treatment and the rapid growth of the disease, has been made researchers to achieve mo MoreTodaymany of the treatment methods are used to treat cancer. Unfortunately, in most cases very poor response is often associated with undesirable side effects. AlphaLack ofresponse to treatment and the rapid growth of the disease, has been made researchers to achieve more effective drugs with fewer side effects. In this study the effect of ethanolic extraction of theBishopand#39;s Flower, Ammi majus on the cancer cell lines Hela and MCF-7 were examined. Cells were cultured in cell culture flasks then transferred to 96 well plates with 10,000 cells. Cells were exposed to different concentrations of Ammi majus. The cytotoxicity of Ammi majus was assessed using the MTT assay after 72 hours. The results of this study showed that ethanolic extract of Ammi majus on Hela cells was significantly reduced the cells grow compared with controls after 72 h. The calculated IC50, was 1/922 mg / ml. The cytotoxicity effect this extract on MCF7 showed that Ammi majus was significantly reduced the MCF7 cells grow compared with controls after 72 h. The calculated IC50, was 10 mg / ml. The effect of this extracts on normal cells (lymphocytes and monocytes in blood) is granted, the results show that the concentration of 10 mg / ml extracts had no effect on normal cells. The results suggest that ethanolic extract of the plant Ammi majus inhibits growth of cancer cells MCF7 and Hela. Manuscript profile -
Open Access Article
47 - The Influence of Drinking Nanosilver on Fetus Death in Rat
وجیهه قربان زاده عبدالغفار عبادیNanosilver, as one of the wondrous scientific outcomes of nanotechnology, has numerous usages in both medicine and pharmacy. In its larger sizes, silver is a metal with low reaction sensitivity but when transformed to very small particles, as small as nanometer, its spe MoreNanosilver, as one of the wondrous scientific outcomes of nanotechnology, has numerous usages in both medicine and pharmacy. In its larger sizes, silver is a metal with low reaction sensitivity but when transformed to very small particles, as small as nanometer, its special properties are intensified. The purpose of this study is the effect of drinking nanosilver on fetus death in rat. In this investigation, 12 pregnant rats were used in experimental environment. The rats were randomly assigned to 3groups of 4. Group1 or the control group, group2, rats which received high dosages (10ppm) of nanosilver through drinking, group3, rats which received low dosage (1ppm) through the same procedure. From the 16th day of pregnancy on, the pregnant rats were treated with drinking nanosilver with the concentration of 10 and 1ppm and the treatment went on till the 22nd day of pregnancy. Later on, the death rate of the babies was monitored in both the treatment and control groups and result were investiged with SPSS software and one way ANOVA statistics analysis. The results of the study depicted the fact that usage of nanosilver through drinking during pregnancy has cytotoxic effects on fetus and would lead to the death of the baby. Manuscript profile -
Open Access Article
48 - Cytotoxicity Effects of Ammi visnaga Extract on Hela and MCF7 Cancer Cell Line vvررررررررررررررر
هدیه پاک فطرت فرخنده نعمتی عبدالحسین شیرویThe discovery of new materials such as anti-microbial and anti-viral and anti-cancer known among plants or those who have recently been discovered can help to treat diseases. In this study, the cytotoxic effect of plant extracts Ammi visnaga extract on Hela and MCF7 cel MoreThe discovery of new materials such as anti-microbial and anti-viral and anti-cancer known among plants or those who have recently been discovered can help to treat diseases. In this study, the cytotoxic effect of plant extracts Ammi visnaga extract on Hela and MCF7 cell lines were analyzed. Different concentrations of ethanolic extract of the Ammi visnaga on cultured blood cells and cancer cell line for 72 hours were examined. The cytotoxicity was evaluated by MTT test. Results are reported as IC50. This study showed that different concentrations of Ammi visnaga extract have cytotoxic effect on Hela and MCF7 cell lines. The percentage of growth inhibition of Hela and MCF7 cells increasing whit whit increasing concentration of test compounds. IC50 value for Hela was 0.57 mg /ml, and for MCF7 was 2mg/ml. The results suggest that ethanolic extract of Ammi visnaga has inhibitory effect on cell growth of MCF7 and Hela cell lines. vv Manuscript profile -
Open Access Article
49 - Cytotoxic Effect of Enteromorpha clatherata Extract on Hela and MCF7 Cancer Cell Lines
ریحانه مهری دیوکلایی فرخنده نعمتی پروین خدارحمی عباسعلی دهپورCancer is the second cause of death in the world. Current therapies for treating cancer are often restricted by short-term efficacy, even lead to the drug resistance. There has been increased interest in the use of natural compounds with chemotherapeutic effects for tre MoreCancer is the second cause of death in the world. Current therapies for treating cancer are often restricted by short-term efficacy, even lead to the drug resistance. There has been increased interest in the use of natural compounds with chemotherapeutic effects for treatment of cancer. In this study, the effects of different concentrations of ethanolic extract of Enteromorpha clatherata, on human breast cancer cell (MCF-7) and cervix cancer cells (Hela) were investigated. Cell were produced in Pasteur Institute and were cultured under controlled conditions with RPMI containing 10% FBS and penicillin streptomycin in cell culture flasks, the cells were transferred to 96 well plates with 10.000 cells. Cells were exposed to different concentrations of extracts (0.156, 0.312, 0.625, 1.25, 2.5, 5, 7.5 and 10 mg/ml) that had been dissolved in RPMI with FBS. Cells cultured with all conditions without the presence of the drug as a control for 72 hours and cell toxicity was assessed with the MTT assay after 72 hours. The result of this study showed that ethanolic extract of Enteromorpha clatherata on both Hela cell and MCF-7 in 0.625 mg/ml concentration consistency reduces the growth of cells significantly in comparison to control group. This extract possesses cytotoxic effects in 0.156, 0.312, 1.25 concentrations and inhibit the growth of cells to 48 %, 66 % and 66 % in Hela and to 56 %, 65% and 70 % in MCF-7 MCF-7 in 0.625 mg/ml. The IC50 is 0.17 mg/ml in Hela and 0.19 mg/ml MCF-7 respectively. The results show that ethanolic extract of Enteromorpha clatherata caused the growth inhibition of cancer cells in Hela and MCF-7. Manuscript profile -
Open Access Article
50 - Biosynthesis of Zinc Oxide Nanoparticles Using Curcumin and Evaluation of its Antioxidant Activity and Cytotoxicity
Elaheh Dianati Vida Hojati Jina Khayatzadeh Saeideh ZafarbalanejadBiocompatibility and stability of zinc oxide nanoparticles (ZnO NPs) synthesized using plants due to their wide applications in biomedical, industrial, cell imaging and biosensors is an interesting research field in the arena of nanotechnology. The present study involve MoreBiocompatibility and stability of zinc oxide nanoparticles (ZnO NPs) synthesized using plants due to their wide applications in biomedical, industrial, cell imaging and biosensors is an interesting research field in the arena of nanotechnology. The present study involved the synthesis of green stable zinc oxide nanoparticles using zinc acetate and aqueous extract of turmeric as a reducing agent. In this characterization study, the antioxidant and cytotoxic activities of the synthesized nanoparticles were investigated by different methods. First, nanoparticles were synthesized by green method and the properties of synthesized nanoparticles were investigated using various spectroscopic and microscopic techniques. Assessment of antioxidant activity was performed using DPPH free radical scavenging and cytotoxicity activity in fibroblasts by MTT method. The involvement of the bioactive compounds of curcumin in the stabilization of zinc oxide nanoparticles was confirmed by FTIR. FESEM electron microscopy showed irregular spherical, rod, and plate-like morphology with uneven surface. Antioxidant activities performed using the free radical scavenging method DPPH showed IC50 values higher than 500 μg/ ml. In addition, biosynthesized zinc oxide nanoparticles showed significant toxic effects on fibroblasts in a concentration and time dependent manner. In general, zinc oxide nanoparticles were synthesized through a sustainable, simple, and environmentally friendly green pathway using curcumin. Biosynthesized zinc oxide nanoparticles showed antioxidant activity and cytotoxicity. Manuscript profile -
Open Access Article
51 - Apoptosis induction of Harmine on Human Colon Cancer Cell Line HT29 and Alteration in Apoptotic Genes Expression P53, Bax and Bcl-2
Reyhaneh Chitbandi Javad Baharara Maryam TehranipourAlkaloids have anti-proliferative and apoptotic stimulatory effects on cancer cells. Harmine is also one of the important alkaloids that have been reported to have anticancer effects. The aim of this study was to investigate the effect of Harmine on apoptosis induction MoreAlkaloids have anti-proliferative and apoptotic stimulatory effects on cancer cells. Harmine is also one of the important alkaloids that have been reported to have anticancer effects. The aim of this study was to investigate the effect of Harmine on apoptosis induction in human HT29 colon cancer cell line and expression alteration of apoptotic genes P53, Bax and Bcl-2. For this purpose, HT29 colon cancer cells were treated with different concentrations of harmine (0, 35, 45, 55, 65 μg/ml) for 24 hours. Then the cell viability was assessed using the MTT assay. Nuclear changes and chromatin condensation were investigated by DAPI staining. Also, apoptosis induction was determined by Annexin V-FITC testing and changes in P53, Bax and Bcl-2 genes expression was assessed by using Real Time PCR. The results showed that the concentration of Harmin reduced the viability of HT29 colon cancer cells. Also, the treatment of HT29 colon cancer cells with Harmin caused morphological changes in the cell nucleus, including chromatin condensation of the nucleus, the formation of apoptotic bodies, and the wrinkling of the cell membrane. All of these morphological features indicate apoptosis. The results of the Annexin test also confirmed this finding; with about 57% of the cells undergo apoptosis. On the other hand, the treatment of HT29 cancer cells with Harmin reduced the expression of Bcl-2 and at the same time increased the expression of P53 and Bax and, in general, reduced the ratio of Bcl-2 to Bax. As a result, Harmin can be used to treat colon cancer cells because of its cytotoxicity, which is effective in inhibiting cell prolifration and inducing apoptosis. Manuscript profile -
Open Access Article
52 - Cytotoxic Effect of Seed Essential Oil of Zizyphus jujuba on K562 Leukemia Cell Line
Elham Ghodousi Dehnavi H. Maghsoudi R. Haji HosseiniEssential oils are complex mixtures of several components endowed with a wide range of biological activities, including anticancer properties. Due to the side effects and the resistance of synthetic drugs, plant products are considered for the treatment of cancer. This MoreEssential oils are complex mixtures of several components endowed with a wide range of biological activities, including anticancer properties. Due to the side effects and the resistance of synthetic drugs, plant products are considered for the treatment of cancer. This study is performed the effect of cytotoxicity of essential oil from the seeds of Zizyphus jujuba (EOSZ) on K562 cell line as a model of chronic myeloid leukemia. In this study, the seeds of Z. jujube were collected from the Isfahan city, and essential oils were extracted by Clevenger apparatus and then the combination by gas chromatography and spectrophotometry were identified. K562 cells were cultured and treated with concentrations of essential oils of 1 to 900 micrograms per milliliter (in 24h, 48h, and 72h). Cytotoxicity of essential oil from the seeds of Zizyphus jujuba (EOSZ) against K562 leukemia cells was estimated by the MTT assay. The absorbance was measured using ELISA plate reader (540 nm) were measured. Then the data were analyzed with the computer software SPSS version 15.0 (SPSS Inc., Chicago, USA), t.test and ANOVA. 24 compounds were identified in the essential oil, the main component of which was Eugenol. Essential oil from the seeds of Zizyphus jujuba (EOSZ), the highest cytotoxic effect in LC50 = 200 micrograms per milliliter and 72 hours after treatment. In other words, the seed essential oil from Zizyphus jujuba (EOSZ) expresses the effects of cellular toxicity on k562 cells, depending on the dose and time. Considering the toxicity of seed essential oil from Zizyphus jujuba (EOSZ), seed essential oil from Zizyphus jujubacan be considered a Potential candidate for further CML studies, and other cancer cells. Manuscript profile -
Open Access Article
53 - Phytochemical Composition, Antioxidant Activity, and Anticancer Effects of Root and Sprout Extracts of Quinoa (Chenopodium quinoa willd.) on AGS Gastric Cancer Cells
Fatemeh Shirali سیّد ابراهیم سیفتیThis study aimed to investigate the phytochemical composition, antioxidant activity, and anticancer effects of root and sprout samples from Multi-hued Bulk, Black, and Titicaca Quinoa varieties (Chenopodium quinoa willd.) on Human Gastric Adenocarcinoma Cell Line (AGS). MoreThis study aimed to investigate the phytochemical composition, antioxidant activity, and anticancer effects of root and sprout samples from Multi-hued Bulk, Black, and Titicaca Quinoa varieties (Chenopodium quinoa willd.) on Human Gastric Adenocarcinoma Cell Line (AGS). These varieties were cultivated using a complete randomized block design with four replications in arid and semi-arid regions of Iran (research field of Yazd University). Root samples were harvested before seed filling from a soil depth of 30 cm. Once the seed germination and filling stages were complete, 100 g of seeds from each variety were randomly selected without hulling to produce sprouts. The highest saponin contents (mg/g dry weight) were observed in the Black root (24.4), followed by the Titicaca root (7.1). The lowest saponin content (0.6) was found in the Black sprout. The phytochemical composition, and antioxidant activity of the hydroethanolic (80:20) extract (obtained using the Soxhlet method), at 100, 250, 500, and 1000 mg/mL were evaluated for root and sprout samples of all three varieties. Based on the results, the Black and Titicaca sprout extract had significantly higher total phenolic (172.68 mgGAE/gDW) and flavonoid (128.17 mgQ/gDW) contents, as well as antioxidant capacity at 1000 mg/mL compared to the corresponding samples from the Multi-hued Bulk variety and the root samples of all three varieties. The lowest and highest DPPH IC50 value was observed in the Black sprout and Multi-hued Bulk root extracts (1.11 and 3.15 mg/mL, respictively). The average cell viability of AGS was observed in the Black sprout extract (33.46) and Titicaca (36.11) varieties at 1000 mg/mL, 72 hours after the treatment, respectively. Findings suggested that the phytochemical and antioxidant properties of Quinoa sprouts, compared to hulled and unhulled seeds of the investigated varieties, could contribute to the development and accessibility of this medicinal plant for patients with cancer. Manuscript profile -
Open Access Article
54 - Antioxidant properties and anticancer activity of Olea europaea L. olive and Ficus carica fruit extracts against pancreatic cancer cell lines
Nadia Dekdouk Souad Ameddah Chawki Bensouici Meghboun Ibtissem Menad Ahmed Fatima MartelOlea europaea (OFE) and Ficus carica (FFE) extracts have shown anticancer activity. In the currents report, we aim to evaluate the phytochemical composition, antioxidant properties and anticancer activity of OFE and FFE against PANC-1 and AsPC-1 pancreatic cancer cell l MoreOlea europaea (OFE) and Ficus carica (FFE) extracts have shown anticancer activity. In the currents report, we aim to evaluate the phytochemical composition, antioxidant properties and anticancer activity of OFE and FFE against PANC-1 and AsPC-1 pancreatic cancer cell lines. OFE possessed higher phenolic, flavonoid content and antioxidant ability than FFE. OFE and FFE induced a cytotoxic effect and their combination resulted in an increased cytotoxicity. OFE showed an antiproliferative effect while FFE had no effect. Combination of the two extracts resulted in an antiproliferative effect similar to that of OFE. OFE showed an antimigratory effect, while FFE exhibited a promigratory effect. Interestingly, combination of the two extracts resulted in an anti-migratory effect similar to that caused by OFE. In view of the findings of this study, these extracts could be considered potential sources of natural compounds with antioxidant and anti-pancreatic cancer effects. The combination of these extracts should be further investigated in the context of pancreatic cancer. Manuscript profile -
Open Access Article
55 - Phytochemical characterization and biological properties of Ocimum sanctum L. and its active phytocomponents: Eugenol and β-caryophyllene
Krupali Trivedi Nilam Parmar Khairah Ansari Vaibhavi Srivastava Nishi Modi Devendrasinh JhalaThis study explored the pharmacognostical and biological properties of Ocimum sanctum L., focusing on its hydromethanolic (HME) and aqueous (AQE) extracts, as well as the bioactive compounds eugenol (EUG) and β-caryophyllene (BCP). Phytochemical analysis revealed a dive MoreThis study explored the pharmacognostical and biological properties of Ocimum sanctum L., focusing on its hydromethanolic (HME) and aqueous (AQE) extracts, as well as the bioactive compounds eugenol (EUG) and β-caryophyllene (BCP). Phytochemical analysis revealed a diverse range of compounds, with HME showing higher levels of phenolics, flavonoids, and tannins compared to AQE. Both extracts exhibited antioxidant activity, while EUG and BCP displayed significant cytotoxicity against MCF-7 cells. Molecular docking studies indicated that EUG has potential binding to catalase. The findings of this study underscore O. sanctum as a valuable source of bioactive compounds with potential antioxidant and anticancer properties. Notably, the extraction method played a crucial role in determining the phytochemical profile. However, further investigations are necessary to fully understand the mechanisms and therapeutic applications of EUG and BCP, particularly as potential catalase inhibitors. Manuscript profile -
Open Access Article
56 - Exploring the biological potential of Cruciata taurica (Pall. ex Willd.) Ehrend.: Evaluating antibacterial, antioxidant, antidiabetic, and cytotoxic properties
Shiva Khalil-Moghaddam Roya Moghimi Thomas Efferth Sajedeh MousavianThis study focused on the biological properties of Cruciata taurica, a plant found in the Irano-Turanian region, which has not been extensively studied in terms of its phytochemical and biological activities. The methanolic extract of the plant was obtained and its cyto MoreThis study focused on the biological properties of Cruciata taurica, a plant found in the Irano-Turanian region, which has not been extensively studied in terms of its phytochemical and biological activities. The methanolic extract of the plant was obtained and its cytotoxic, antibacterial, antidiabetic, and antioxidant properties were investigated. The antibacterial activity of the fractions was evaluated using broth microdilution method, while the antioxidant properties were assessed through the DPPH assay and the cytotoxic properties were determined using the RRA assay. This study revealed that the residual aqueous fraction exhibited the highest extraction yield and the most potent antioxidant activity. The EtOAc fraction showed strong antibacterial activity against certain bacteria, while the n-hexane fraction exhibited greater antidiabetic effects. Additionally, the EtOAc fraction was found to be the most cytotoxic. Overall, the study suggested that C. taurica has potential therapeutic applications due to its various biological properties. Manuscript profile