The cytotoxicity of culture extracts of indigenous Actinobacteria of Iran using Artemia urmiana
Subject Areas :
Applied Microbiology
Sanaz Imanian
1
,
Ali Mehrvar
2
,
Javad Hamedi
3
,
Hossein Samadi Kafil
4
,
Naser Eivazian Kari
5
1 - Department of Plant Protection,, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
2 - Department of Plant Protection,, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz- Iran.
3 - Professor in Microbiology, Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran
4 - Department of Microbiology and Virology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5 - Department of Plant Protection, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran
Received: 2022-06-03
Accepted : 2022-11-21
Published : 2022-12-06
Keywords:
Cytotoxicity,
bioassay,
Brine shrimp,
Actinobacteria,
Abstract :
Background & Objectives: Actinobacteria are very important in the world in terms of the production of metabolites with various biological effects. This study was conducted with the aim of investigating the toxicity of the fermented extract obtained from Actinobacteria isolated from some biological resources of Iran on Artemia urmiana. These isolates were deposited in University of Tehran Microorganisms Collection (UTMC).Materials & Methods: Forty-eight -76°C actinobacterial isolates were revived using ISP2 agar culture medium. Two bacterial discs were inoculated in ISP2 broth medium as a pre-cultivation medium. After 48 hours, 10 ml of the liquid was inoculated into the fermentation culture medium and heated for 7 days in a shaker incubator with 180 rpm, temperature of 28°C and pH of 7.2 ± 0.2. The fermentation broth was extracted using ethyl acetate, the solvent was evaporated using a low-pressure rotary evaporator. The toxicity of fermentation broth extracts was assayed against 40-hour Artemia urmiana nauplii.Results: According to the toxicity classification, among the all 48 isolates, 64.58% were highly toxic, 22.91% were moderate toxic, and 12.5% were in the low toxicity compounds group.Conclusion: The results showed that more than half of the tested extracts had very high lethality on Artemia urmiana in a short time. Comparing the LC50 and LT50 values of the extracts with similar studies, it was found that these extracts have a significant biological activity and can be used as a rich source in the production of metabolites with proper biological effects.
References:
Asem A, Eimanifar A. Updating historical record on brine shrimp Artemia (Crustacea: Anostraca) from Urmia Lake (Iran) in the first half of the10th century AD. Int J Aquatic Sci. 2016; 7: 3-5.
Chan W, Shaughnessy AE, van den Berg CP, Garson MJ, Cheney KL. The validity of brine shrimp (Artemia sp.) toxicity assays to assess the ecological function of marine natural products. J Chem Ecol. 2021; 47(10): 834-46.
Hamrun N, Nabilah T, Hasyim R, Ruslin M, Dammar I, As MA. Toxicity test of bioactive red alga extract Eucheuma spinosum on shrimp Artemia salina leach. Sys Rev Pharm. 2020; 11(5): 672-6.
Karchesy YM, Kelsey RG, Constantine G, Karchesy JJ. Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay. Springerplus. 2016; 5(1): 1-9.
Vivekanandhan P, Swathy K, Kalaimurugan D, Ramachandran M, Yuvaraj A, Kumar AN, et al. Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms. Plos One. 2020; 15(5): e0232172.
Kasanah N, Triyanto T. Bioactivities of halometabolites from marine actinobacteria. Biomolecules. 2019; 9(6): 225-43.
Dhakal D, Pokhrel AR, Shrestha B, Sohng JK. Marine rare actinobacteria: isolation, characterization, and strategies for harnessing bioactive compounds. Front Microbiol. 2017; 8: 1106.
Law JW-F, Pusparajah P, Ab Mutalib N-S, Wong SH, Goh B-H, Lee L-H. A review on mangrove actinobacterial diversity: the roles of Streptomyces and novel species discovery. Prog Microb Mol Biol. 2019; 2(1).
Tiwari K, Upadhyay DJ, Mösker E, Süssmuth R, Gupta RK. Culturable bioactive actinomycetes from the great Indian Thar Desert. Annal Microbiol. 2015; 65(4): 1901-14.
Borah A, Thakur D. Phylogenetic and functional characterization of culturable endophytic Actinobacteria associated with Camellia spp. for growth promotion in commercial tea cultivars. Front Microbiol. 2020; 11: 318.
Bérdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot. 2012; 65(8): 385-95.
Chaudhary HS, Soni B, Shrivastava AR, Shrivastava S. Diversity and versatility of Actinomycetes and its role in antibiotic production. J Appl Pharm Sci. 2013; 3(8): 83-94.
Ratnakomala S, Perwitasari U, editors. The amylase production by Actinobacteria isolated from rumen fluid. IOP Conference Series: Earth Environ Sci; 2020; 439(1): 012019.
Xiong Y-W, Ju X-Y, Li X-W, Gong Y, Xu M-J, Zhang C-M, et al. Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180. Int J Biol Macromol. 2020; 153: 1176-85.
Sivalingam P, Hong K, Pote J, Prabakar K. Extreme environment Streptomyces: potential sources for new antibacterial and anticancer drug leads? Int J Microbiol. 2019; e5283948.
Fateh M, Habiba Z, Khaled B, Billel M, Youcef N, Abderrahmane B. Immunostimulatory activity of intracellular lectin extract from Actinomycete Micromonospora aurantiaca. Int. J. Toxicol. Pharmacol. Res. 2015; 7(6): 264-268.
Azimi S, Basei Salehi M, Bahador N. Isolation and identification of Streptomyces ramulosus from soil and determination of antimicrobial property of its pigment. Mod Med Lab J. 2017; 1(1): 36-41.
Laassami A, Yekkour A, Meklat A, Djemouai N, Zitouni A, Mokrane S, et al. Actinobacteria associated with vineyard soils of algeria: classification, antifungal potential against grapevine trunk pathogens and plant growth-promoting features. Curr Microbiol. 2020; 77(10): 2831-40.
Sarwar A, Latif Z, Zhang S, Zhu J, Zechel DL, Bechthold A. Biological control of potato common scab with rare isatropolone C compound produced by plant growth promoting Streptomyces A1RT. Front Microbiol. 2018; 9: 1126.
Safaeian S, Nouhi AA, Oryan S. Studies on cytotoxic activity of marine Actionomycetes from Persian Gulf on Artemia franciscana and Artemia urmiana. Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology. 2005; 141(3): 133-139.
Hamedi J, Kafshnouchi M, Ranjbaran M. A study on actinobacterial diversity of Hampoeil cave and screening of their biological activities. Saudi J Biol Sci. 2019; 26(7): 1587-95.
Mensah PK, Palmer CG, Muller WJ. Lethal and sublethal effects of pesticides on aquatic organisms: the case of a freshwater shrimp exposure to Roundup®. Pesticides: Toxic Aspects, In Tech Publica‐tions, Rijeka, Croatia. 2014: 163-85.
Mehrvar A. Studies on the nucleopolyhedrovirus of Helicoverpa armigera (Hübner): evaluation of its geographic isolates. LAP LAMBERT Academic Publishing; 2012.
Shapiro M, Argauer R. Relative effectiveness of selected stilbene optical brighteners as enhancers of the beet armyworm (Lepidoptera: Noctuidae) nuclear polyhedrosis virus. J Econ Entomol. 2001; 94(2): 339-43.
Hamidi M, Jovanova B, Panovska T. Toxicоlogical evaluation of the plant products using brine shrimp (Artemia salina L.) model. Maced Pharm Bull. 2014; 60(1): 9-18.
Sarrami S, Hamedi J, Mohammadipanah F, Rezayat Sorkhabadi SM. Study of cytotoxic effects of metabolites produced by Actinomycetes. Jundishapur Sci Med J. 2014; 13(3): 347-355. [In Persian].
Rahayu S, Fitri L, Ismail YS. The endophytic Actinobacterial toxicity test of ginger (Zingiber officinale Roscoe) used the BSLT (Brine Shrimp Lethality Test) method. Elkawnie: J Islamic Sci Technol. 2021; 7(1): 19-29.
Xiong L, Li J, Kong F. Streptomyces sp. 173, an insecticidal micro‐organism from marine. Lett Appl Microbiol. 2004; 38(1): 32-7.
Ghavamizadeh M, Mohammadi J, Mirzaei A, Sadeghi H, Akbartabar M. Cytotoxicity of Dorema auchrei, Achillea millefolium and Artemisia aucheri by Artemia. Armaghane Danesh. 2013; 18(5): 389-99.
Mashjoor S, Yousefzadi M. Cytotoxic effects of three Persian Gulf species of Holothurians. Iranian J Vet Res. 2019; 20(1): 19-26.
_||_
Asem A, Eimanifar A. Updating historical record on brine shrimp Artemia (Crustacea: Anostraca) from Urmia Lake (Iran) in the first half of the10th century AD. Int J Aquatic Sci. 2016; 7: 3-5.
Chan W, Shaughnessy AE, van den Berg CP, Garson MJ, Cheney KL. The validity of brine shrimp (Artemia sp.) toxicity assays to assess the ecological function of marine natural products. J Chem Ecol. 2021; 47(10): 834-46.
Hamrun N, Nabilah T, Hasyim R, Ruslin M, Dammar I, As MA. Toxicity test of bioactive red alga extract Eucheuma spinosum on shrimp Artemia salina leach. Sys Rev Pharm. 2020; 11(5): 672-6.
Karchesy YM, Kelsey RG, Constantine G, Karchesy JJ. Biological screening of selected Pacific Northwest forest plants using the brine shrimp (Artemia salina) toxicity bioassay. Springerplus. 2016; 5(1): 1-9.
Vivekanandhan P, Swathy K, Kalaimurugan D, Ramachandran M, Yuvaraj A, Kumar AN, et al. Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and non-targeting organisms. Plos One. 2020; 15(5): e0232172.
Kasanah N, Triyanto T. Bioactivities of halometabolites from marine actinobacteria. Biomolecules. 2019; 9(6): 225-43.
Dhakal D, Pokhrel AR, Shrestha B, Sohng JK. Marine rare actinobacteria: isolation, characterization, and strategies for harnessing bioactive compounds. Front Microbiol. 2017; 8: 1106.
Law JW-F, Pusparajah P, Ab Mutalib N-S, Wong SH, Goh B-H, Lee L-H. A review on mangrove actinobacterial diversity: the roles of Streptomyces and novel species discovery. Prog Microb Mol Biol. 2019; 2(1).
Tiwari K, Upadhyay DJ, Mösker E, Süssmuth R, Gupta RK. Culturable bioactive actinomycetes from the great Indian Thar Desert. Annal Microbiol. 2015; 65(4): 1901-14.
Borah A, Thakur D. Phylogenetic and functional characterization of culturable endophytic Actinobacteria associated with Camellia spp. for growth promotion in commercial tea cultivars. Front Microbiol. 2020; 11: 318.
Bérdy J. Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot. 2012; 65(8): 385-95.
Chaudhary HS, Soni B, Shrivastava AR, Shrivastava S. Diversity and versatility of Actinomycetes and its role in antibiotic production. J Appl Pharm Sci. 2013; 3(8): 83-94.
Ratnakomala S, Perwitasari U, editors. The amylase production by Actinobacteria isolated from rumen fluid. IOP Conference Series: Earth Environ Sci; 2020; 439(1): 012019.
Xiong Y-W, Ju X-Y, Li X-W, Gong Y, Xu M-J, Zhang C-M, et al. Fermentation conditions optimization, purification, and antioxidant activity of exopolysaccharides obtained from the plant growth-promoting endophytic actinobacterium Glutamicibacter halophytocola KLBMP 5180. Int J Biol Macromol. 2020; 153: 1176-85.
Sivalingam P, Hong K, Pote J, Prabakar K. Extreme environment Streptomyces: potential sources for new antibacterial and anticancer drug leads? Int J Microbiol. 2019; e5283948.
Fateh M, Habiba Z, Khaled B, Billel M, Youcef N, Abderrahmane B. Immunostimulatory activity of intracellular lectin extract from Actinomycete Micromonospora aurantiaca. Int. J. Toxicol. Pharmacol. Res. 2015; 7(6): 264-268.
Azimi S, Basei Salehi M, Bahador N. Isolation and identification of Streptomyces ramulosus from soil and determination of antimicrobial property of its pigment. Mod Med Lab J. 2017; 1(1): 36-41.
Laassami A, Yekkour A, Meklat A, Djemouai N, Zitouni A, Mokrane S, et al. Actinobacteria associated with vineyard soils of algeria: classification, antifungal potential against grapevine trunk pathogens and plant growth-promoting features. Curr Microbiol. 2020; 77(10): 2831-40.
Sarwar A, Latif Z, Zhang S, Zhu J, Zechel DL, Bechthold A. Biological control of potato common scab with rare isatropolone C compound produced by plant growth promoting Streptomyces A1RT. Front Microbiol. 2018; 9: 1126.
Safaeian S, Nouhi AA, Oryan S. Studies on cytotoxic activity of marine Actionomycetes from Persian Gulf on Artemia franciscana and Artemia urmiana. Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology. 2005; 141(3): 133-139.
Hamedi J, Kafshnouchi M, Ranjbaran M. A study on actinobacterial diversity of Hampoeil cave and screening of their biological activities. Saudi J Biol Sci. 2019; 26(7): 1587-95.
Mensah PK, Palmer CG, Muller WJ. Lethal and sublethal effects of pesticides on aquatic organisms: the case of a freshwater shrimp exposure to Roundup®. Pesticides: Toxic Aspects, In Tech Publica‐tions, Rijeka, Croatia. 2014: 163-85.
Mehrvar A. Studies on the nucleopolyhedrovirus of Helicoverpa armigera (Hübner): evaluation of its geographic isolates. LAP LAMBERT Academic Publishing; 2012.
Shapiro M, Argauer R. Relative effectiveness of selected stilbene optical brighteners as enhancers of the beet armyworm (Lepidoptera: Noctuidae) nuclear polyhedrosis virus. J Econ Entomol. 2001; 94(2): 339-43.
Hamidi M, Jovanova B, Panovska T. Toxicоlogical evaluation of the plant products using brine shrimp (Artemia salina L.) model. Maced Pharm Bull. 2014; 60(1): 9-18.
Sarrami S, Hamedi J, Mohammadipanah F, Rezayat Sorkhabadi SM. Study of cytotoxic effects of metabolites produced by Actinomycetes. Jundishapur Sci Med J. 2014; 13(3): 347-355. [In Persian].
Rahayu S, Fitri L, Ismail YS. The endophytic Actinobacterial toxicity test of ginger (Zingiber officinale Roscoe) used the BSLT (Brine Shrimp Lethality Test) method. Elkawnie: J Islamic Sci Technol. 2021; 7(1): 19-29.
Xiong L, Li J, Kong F. Streptomyces sp. 173, an insecticidal micro‐organism from marine. Lett Appl Microbiol. 2004; 38(1): 32-7.
Ghavamizadeh M, Mohammadi J, Mirzaei A, Sadeghi H, Akbartabar M. Cytotoxicity of Dorema auchrei, Achillea millefolium and Artemisia aucheri by Artemia. Armaghane Danesh. 2013; 18(5): 389-99.
Mashjoor S, Yousefzadi M. Cytotoxic effects of three Persian Gulf species of Holothurians. Iranian J Vet Res. 2019; 20(1): 19-26.
