The study of alpha-tocopherol cytotoxicity on canine mammary gland cancer cells (CF41.Mg) invitro

Subject Areas : Journal of Comparative Pathobiology

ریحانه سفیدابی، پژمان مرتضوی . ¹ (.)

Received: 2016-02-25 Accepted : 2016-02-25 Published : 2016-02-20

Keywords: Cytotoxicity, Alpha-tocopherol, Mammary Gland Cancer, Canine,

Abstract :

Breast cancer is the most common cause of cancer in female dogs. Created mutations in genes associated with cancer and oxidative modification of proteins and reactive aldehydes are key events that can increase cancer risk. The role of antioxidants in prevention and treatment of cancers have been reported by several studies. The aim of this study was to evaluate invitro, the antiproliferative effect of alpha-tocopherol against canine mammary gland carcinoma cell line (CF41.Mg). CF41.Mg cells were cultured in RPMI 1640 medium. The cells were treated with different concentration of alpha-tocopherol for 24, 48 and 72 h. Antiproliferative effect of alpha-tocopherol was investigated using MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-tetrazolium bromide) assay. The results indicated that alpha-tocopherol inhibited proliferation of canine mammary gland carcinoma cells so that treatment with 100 microM alpha-tocopherol for 72 h resulted in a significant decrease in cell viability. The alpha-tocopherol showed less effect after 48 h. The results of this study confirm the inhibitory effect of alpha-tocopherol on canine breast cancer cells (CF41.Mg).