Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in North Khorasan province
Subject Areas :
Medicinal Plants
Ali Koohestanian
1
,
Maryam Taghizadeh
2
,
Maliheh Samadi Kazemi
3
,
Ahmad Asgharzadeh
4
,
Seyedeh Faezeh Taghizadeh
5
1 - PhD student, Department of Horticultural Sciences, Faculty of Agriculture, Shirvan branch, Islamic Azad University, Shiravan, Iran
2 - 2Assistant Professor, Department of Agriculture, Faculty of Agriculture, Shirvan branch, Islamic Azad University, Shiravan, Iran
3 - Assistant Professor, Department of Chemistry, Faculty of Science, Bojnord Branch, Islamic Azad University, Bojnord, Iran
4 - Assistant Professor, Department of Horticultural Sciences, Faculty of Agriculture, Shirvan branch, Islamic Azad University, Shiravan, Iran
5 - Postdoctoral, Pharmaceutical Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Iran
Received: 2022-02-13
Accepted : 2022-06-11
Published : 2022-11-27
Keywords:
MIC,
MBC,
Cytotoxicity,
Antibacterial,
Rosa persica L,
Abstract :
The aim of this study was to Phytochemical, antimicrobial and cytotoxicity effects of Rosa persica L. extracts in a complete randomized design with three replications. R. persica L. was collected from Shirvan city located in North Khorasan province in the late summer of 2019 . The methanol, petroleum ether, ethyl acetate and water extracts were prepared by maceration method and were analyzed by using the liquid chromatography-mass spectrometer system, it was determined that the methanolic extract had the highest amounts of rosmarinic acid compounds. Antimicrobial effects of different extracts were investigated by determining the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). Antimicrobial assays against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, Bacillus cereus, Pseudomonas aeruginosa, Salmonella typhi, Serratia marcescens, Escherichia coli, Candida albicans, Aspergillus niger showed that in all treatments, gram-negative bacteria were more resistance in compare to gram positive bacteria. In all extracts, the most sensitive strain was S. aureus with MIC and MBC of 10.89±0.13 and 19.34±0.33 μg/ml in the methanolic extract, respectively. Methanolic extracts of aerial parts had significantly stronger antimicrobial effects, respectively. Based on the results of cytotoxicity of different extracts on MCF-7, DU-145, PC3, A2780, C26, U-87-MG, MDA-MB-231, and Hela, it was found that Methanolic extract had the most cytotoxic effects on all cell lines studied. The lowest effects of cytotoxicity were related to the aqueous extract of aerial parts. In all treatments and cell lines, the IC50 was less than 400 μg/ml. The biochemical and biological activities of R. persia may be related to its phenolic compounds, so further studies is recommended.
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Yi, O., Jovel, E.M., Towers, G.N., Wahbe, T.R., Cho, D. 2007. Antioxidant and antimicrobial activities of native Rosa from British Columbia, Canada. International Journal of Food Sciences and Nutrition, 58: 178-189.
Zu, Y., Yu, H., Liang, L., Fu, Y., Efferth, T., Liu, X., Wu, N. 2010. Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules, 15: 3200-3210.
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Ayati, Z., Amiri, M.S., Ramezani, M., Delshad, E., Sahebkar, A., Emami, S.A. 2018. Phytochemistry, traditional uses and pharmacological profile of Rose hip: A review. Current pharmaceutical design, 24: 4101-4124.
Azadmanesh, R., Tatari, M., Asgharzade, A., Taghizadeh, S.F., Shakeri, A. 2021. GC/MS profiling and biological traits of Eucalyptus globulus essential oil exposed to solid lipid nanoparticle (SLN). Journal of essential oil bearing plants, 24: 863-878.
Chen, Y., Liu, Z-J., Liu, J., Liu, L-K., Zhang, E-S., Li, W-L. 2015. Inhibition of metastasis and invasion of ovarian cancer cells by crude polysaccharides from Rosa roxburghii tratt in vitro. Asian Pacific Journal of Cancer Prevention, 15(23):10351-4.
Cheng, B.C.Y., Fu, X.-Q., Guo, H., Li, T., Wu, Z.-Z., Chan, K., Yu, Z.-L. 2016. The genus Rosa and arthritis: Overview on pharmacological perspectives. Pharmacological research 114: 219-234.
Djenane, D., Aïder, M., Yangüela, J., Idir, L., Gómez, D., Roncalés, P. 2012. Antioxidant and antibacterial effects of Lavandula and Mentha essential oils in minced beef inoculated with coli O157: H7 and S. aureus during storage at abuse refrigeration temperature. Meat Science, 92: 667-674.
El Omari, K., Hamze, M., Alwan, S., Osman, M., Jama, C., Chihib, N.-E. 2019. In-vitro evaluation of the antibacterial activity of the essential oils of Micromeria barbata, Eucalyptus globulus and Juniperus excelsa against strains of Mycobacterium tuberculosis (including MDR), Mycobacterium kansasii and Mycobacterium gordonae. Journal of infection and public health, 12: 615-618.
Emami, A., Mousavi, Z., Ramezani, V., Shoeibi, S., Rastegar, H., Amirahmadi, M., Emami, I. 2017. Residue levels and risk assessment of pesticides in Pistachio nuts in Iran. Iranian Journal of Toxicology, 11: 1-6.
Graziano, M.C., Cuzzullin, G.C., Franco, H.O., Schwartz-Filho, E.D., de Andrade, F.C. 2015. Groppo statins and antimicrobial effects: simvastatin as a potential drug against Staphylococcus aureus biofilm, PLoS One, 10: 128-198.
Jiménez, S., Gascón, S., Luquin, A., Laguna, M, Ancin-Azpilicueta, C., Rodríguez-Yoldi, MJ. 2016. Rosa canina extracts have antiproliferative and antioxidant effects on caco-2 human colon cancer. PloS one. 11: 70-81.
Ma, Y., Wang, Y., Zhang, H., Sun, W., Li, Z., Zhang, F., Zhang, H., Chen, F., Zhang, H., An, J. 2020. Antimicrobial mechanism of strictinin isomers extracted from the root of Rosa roxburghii Tratt (Ci Li Gen). Journal of ethnopharmacology, 250: 112498.
Mármol I, Sánchez-de-Diego C, Jiménez-Moreno N, Ancín-Azpilicueta C, Rodríguez-Yoldi MJ. 2017. Therapeutic applications of rose hips from different Rosa International Journal of Molecular Sciences, 18(6):1137.
Nejatbakhsh F, Shirbeigi L, Rahimi R, Abolhassani H. 2016. Review of local herbal compounds found in the Iranian traditional medicine known to optimise male fertility. Andrologia, 48(8):850-9.
Nikolova, G., Karamalakova, Y., Kovacheva, N., Stanev, S., Zheleva, A., Gadjeva, V. 2016. Protective effect of two essential oils isolated from Rosa damascena and Lavandula angustifolia Mill, and two classic antioxidants against L-dopa oxidative toxicity induced in healthy mice. Regulatory Toxicology and Pharmacology, 81: 1-7.
Sadraei, H., Asghari, G., Emami, S. 2013. Inhibitory effect of Rosa damascena flower essential oil, geraniol and citronellol on rat ileum contraction. Research in pharmaceutical sciences, 8(1):17.
Sarabhai, S., Harjai, K., Sharma, P., Capalash, N. 2015. Ellagic acid derivatives from Terminalia chebula increase the susceptibility of Pseudomonas aeruginosa to stress by inhibiting polyphosphate kinase. Journal of applied microbiology, 118: 817-825.
Shakeri, A., Akhtari, J., Soheili, V., Taghizadeh, S.F., Sahebkar, A., Shaddel, R., Asili, J. 2017. Identification and biological activity of the volatile compounds of Glycyrrhiza triphylla & CA Mey. Microbial pathogenesis, 109: 39-44.
Shakeri, A., D’Urso, G., Taghizadeh, S.F., Piacente, S., Norouzi, S., Soheili, V., Asili, J., Salarbashi, D. 2019. LC-ESI/LTQOrbitrap/MS/MS and GC–MS profiling of Stachys parviflora and evaluation of its biological activities. Journal of pharmaceutical and biomedical analysis, 168: 209-216.
Snene, A., El Mokni, R., Jmii, H., Jlassi, I., Jaïdane, H., Falconieri, D., Piras, A., Dhaouadi, H., Porcedda, S., Hammami, S. 2017. In vitro antimicrobial, antioxidant and antiviral activities of the essential oil and various extracts of wild (Daucus virgatus (Poir.) Maire) from Tunisia. Industrial Crops and Products, 109: 109-115.
Taghizadeh, S.F., Rezaee, R., Mehmandoust, M., Badibostan, H., Karimi, G. 2020. Assessment of in vitro bioactivities of Pis v 1 (2S albumin) and Pis v 2.0101 (11S globulin) proteins derived from pistachio (Pistacia vera). Journal of Food Measurement and Characterization, 14: 1054-1063.
Taghizadeh, S.F., Rezaee, R., Mehmandoust, M., Madarshahi, F.S., Tsatsakis, A., Karimi, G. 2019. Coronatine elicitation alters chemical composition and biological properties of Cumin seed essential oil. Microbial pathogenesis, 130: 253-258.
Yi, O., Jovel, E.M., Towers, G.N., Wahbe, T.R., Cho, D. 2007. Antioxidant and antimicrobial activities of native Rosa from British Columbia, Canada. International Journal of Food Sciences and Nutrition, 58: 178-189.
Zu, Y., Yu, H., Liang, L., Fu, Y., Efferth, T., Liu, X., Wu, N. 2010. Activities of ten essential oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 cancer cells. Molecules, 15: 3200-3210.
